CN103808774A - Average red blood cell glucose concentration - Google Patents
Average red blood cell glucose concentration Download PDFInfo
- Publication number
- CN103808774A CN103808774A CN201410066276.XA CN201410066276A CN103808774A CN 103808774 A CN103808774 A CN 103808774A CN 201410066276 A CN201410066276 A CN 201410066276A CN 103808774 A CN103808774 A CN 103808774A
- Authority
- CN
- China
- Prior art keywords
- concentration
- glucose concentration
- glucose
- hematocrit value
- red blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000008103 glucose Substances 0.000 title claims abstract description 67
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 66
- 210000003743 erythrocyte Anatomy 0.000 title abstract description 16
- 210000004369 blood Anatomy 0.000 claims abstract description 35
- 239000008280 blood Substances 0.000 claims abstract description 35
- 238000005534 hematocrit Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000005259 measurement Methods 0.000 claims description 2
- 206010022489 Insulin Resistance Diseases 0.000 abstract description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 239000003146 anticoagulant agent Substances 0.000 description 4
- 229940127219 anticoagulant drug Drugs 0.000 description 4
- 239000006188 syrup Substances 0.000 description 4
- 235000020357 syrup Nutrition 0.000 description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 3
- 229960002897 heparin Drugs 0.000 description 3
- 229920000669 heparin Polymers 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 208000007536 Thrombosis Diseases 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000013389 whole blood assay Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses average red blood cell glucose concentration. A method for measuring the glucose concentration inside red blood cells is not available in the field of medical detection. Three data of whole blood glucose concentration, plasma glucose concentration and hematocrit value of the same one blood sample can be measured by using the method. The average red blood cell glucose concentration is calculated by substituting the following formula of the invention: average red blood cell glucose concentration=[whole blood glucose concentration-plasma glucose concentration*(1-hematocrit value)/hematocrit value. The average red blood cell glucose concentration has important significance on calibration of a portable glucometer and study on insulin resistance.
Description
Technical field
in medical testblood-glucose is measured, and can have plasma glucose mensuration, serum glucose mensuration, full blood glucose to measure according to the difference of sample used.But there is no the simply assay method for clinical mean corpuscular concentration of glucose.
Background technology
Along with the incidence of disease of China's diabetes is more and more higher, the mensuration of glucose is more and more important for diagnosis, treatment, the research of diabetes.The mensuration of mean corpuscular concentration of glucose is for the correction of blood glucose meter, and the research of insulin resistance is all very important.This method can calculate mean corpuscular concentration of glucose by plasma glucose concentration, full blood glucose concentration, hematocrit value.
Summary of the invention
first talk aboutwhat is plasma glucose, full blood glucose, hematocrit value.
plasma glucose: gather anticoagulant heparin whole blood, the concentration of glucose of measuring through centrifugal separation plasma.
Full blood glucose: the red blood cell in whole blood is all smashed, and whole blood becomes pure solution, and then the concentration of glucose of measuring.
Hematocrit value: refer to that red blood cell accounts for the number percent of whole blood volume.The ratio of its reflection red blood cell and blood plasma.By the blood that adds a small amount of anti-coagulants and mix, pack specific volume pipe special, tool scale into, the centrifugal half an hour of speed turning with per minute 3000, the each composition of visible blood is pressed gravity difference and layering, and upper strata weak yellow liquid is blood plasma, and the opaque kermesinus thrombus of lower floor is red blood cell, skim tunica albuginea between red blood cell and blood plasma is leucocyte and blood platelet, account for 1%, now, red blood cell accounts for whole blood volume percent and is hematocrit value.The now compressed zero-clearance of red blood cell of cause, claims again packed cell volume.The hematocrit value of health adult of China, man 40～50%, female 37～48%.
Instrument
1. German EKF diagnostic companies malaga sugar/lactic acid analyser (this instrument is Enzyme Electrode, can use blood plasma, serum, whole blood to measure concentration of glucose for sample)
2. hypervelocity hydro-extractor (for centrifugal separation plasma and mensuration hematocrit value).
This method is after utilizing whole blood centrifugal, and whole blood can be divided into two-layer substantially, and upper strata weak yellow liquid is blood plasma, and the opaque kermesinus thrombus of lower floor is red blood cell.Skim tunica albuginea between red blood cell and blood plasma is leucocyte and blood platelet, accounts for 1% negligible.Concentration of glucose and volume, full blood glucose concentration and volume that can practical measurement upper plasma layer, ask the concentration of lower floor's red blood cell layer glucose.Like the syrup of two glasss of variable concentrations, the concentration of the syrup of known one glass of high concentration and volume, also know two glasss of concentration and volumes after mixing, also know the volume of low concentration syrup, ask the concentration of low concentration syrup.
This method plasma glucose concentration used, full blood glucose concentration are all used same instrument, German EKF diagnostic companies malaga sugar/lactic acid analysis-e/or determining.
If 1ml anticoagulant heparin whole blood, we use German EKF diagnostic companies malaga sugar/lactic acid analyser can actually record its plasma glucose concentration, full blood glucose concentration, then determine hematocrit value (HCT) with hydro-extractor
So:
Plasma glucose concentration × (1ml-1ml × hematocrit value)+mean corpuscular concentration of glucose × (1ml × hematocrit value)=full blood glucose concentration × 1ml
Mean corpuscular concentration of glucose=[full blood glucose concentration × 1ml-plasma glucose concentration × (1ml-1ml × hematocrit value)] ÷ (1ml × hematocrit value)
Can be reduced to:
Mean corpuscular concentration of glucose=[full blood glucose concentration-plasma glucose concentration × (1-hematocrit value)] ÷ hematocrit value
The unit of mean corpuscular concentration of glucose is consistent with full blood glucose concentration, plasma glucose concentration unit for calculating.
For example: certain human blood sample anticoagulant heparin, we first mix whole blood to gather whole blood assay full blood glucose concentration and record 5.00mmol/L, a residual blood part is measured hematocrit value and records 45%, and it is 5.70mmol/L that all the other centrifugal blood separated plasmas are measured plasma glucose concentration.In order to reduce the impact of haemocyte metabolism on concentration of glucose, above three numerical value will complete in the shortest timing.Above numerical value substitution formula: this person's mean corpuscular concentration of glucose=[5.00mmol/L-5.70mmol/L × (1-0.45)] ÷ 0.45 ≈ 4.14
This person's mean corpuscular concentration of glucose is 4.14 mmol/L.
Claims (1)
1. the present invention is for the indirect determination of mean corpuscular concentration of glucose, present stage does not also have a kind of method of measuring mean corpuscular concentration of glucose to be applied to clinical, my method of invention is to utilize same sample at its plasma glucose concentration of the shortest timing, full blood glucose concentration, calculate mean corpuscular concentration of glucose with hematocrit value by these three measurement results, its formula is: mean corpuscular concentration of glucose=[full blood glucose concentration × 1ml-plasma glucose concentration × (1ml-1ml × hematocrit value)] ÷ (1ml × hematocrit value)
Can be reduced to:
Mean corpuscular concentration of glucose=[full blood glucose concentration-plasma glucose concentration × (1-hematocrit value)] ÷ hematocrit value
The unit of mean corpuscular concentration of glucose is consistent with full blood glucose concentration, plasma glucose concentration unit for calculating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410066276.XA CN103808774B (en) | 2014-02-26 | 2014-02-26 | Mean corpuscular concentration of glucose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410066276.XA CN103808774B (en) | 2014-02-26 | 2014-02-26 | Mean corpuscular concentration of glucose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103808774A true CN103808774A (en) | 2014-05-21 |
| CN103808774B CN103808774B (en) | 2016-01-13 |
Family
ID=50705857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410066276.XA Expired - Fee Related CN103808774B (en) | 2014-02-26 | 2014-02-26 | Mean corpuscular concentration of glucose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103808774B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107209174A (en) * | 2014-11-25 | 2017-09-26 | 聚合物技术系统公司 | System and method by alternating current impedance phase-angle detection to determine electrochemistry hematocrit value |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036079A (en) * | 1988-03-21 | 1989-10-04 | 罗伯特·A·利费恩 | Measure the method for haemoglobin |
| WO1999012021A1 (en) * | 1997-08-29 | 1999-03-11 | Han Chi Neng Arthur | Analyte detection systems |
| CN1605869A (en) * | 2003-10-09 | 2005-04-13 | 堀口升 | Blood examination method |
| JP2005160782A (en) * | 2003-12-03 | 2005-06-23 | Hitachi Ltd | Blood sugar level measuring instrument |
| CN102419367A (en) * | 2010-09-24 | 2012-04-18 | 株式会社堀场制作所 | Whole blood immunity measuring device and whole blood immunity measuring method |
-
2014
- 2014-02-26 CN CN201410066276.XA patent/CN103808774B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036079A (en) * | 1988-03-21 | 1989-10-04 | 罗伯特·A·利费恩 | Measure the method for haemoglobin |
| WO1999012021A1 (en) * | 1997-08-29 | 1999-03-11 | Han Chi Neng Arthur | Analyte detection systems |
| CN1605869A (en) * | 2003-10-09 | 2005-04-13 | 堀口升 | Blood examination method |
| JP2005160782A (en) * | 2003-12-03 | 2005-06-23 | Hitachi Ltd | Blood sugar level measuring instrument |
| CN102419367A (en) * | 2010-09-24 | 2012-04-18 | 株式会社堀场制作所 | Whole blood immunity measuring device and whole blood immunity measuring method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107209174A (en) * | 2014-11-25 | 2017-09-26 | 聚合物技术系统公司 | System and method by alternating current impedance phase-angle detection to determine electrochemistry hematocrit value |
| CN107209174B (en) * | 2014-11-25 | 2020-01-21 | 聚合物技术系统公司 | System and method for determining electrochemical hematocrit by alternating current impedance phase angle detection |
| US11680925B2 (en) | 2014-11-25 | 2023-06-20 | Polymer Technology Systems, Inc. | Systems and methods for electrochemical hematocrit determination by alternate current impedance phase angle determinations |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103808774B (en) | 2016-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Marlar et al. | Activated partial thromboplastin time monitoring of unfractionated heparin therapy: issues and recommendations | |
| Lippi et al. | Haemolysis: an overview of the leading cause of unsuitable specimens in clinical laboratories | |
| Meng et al. | Pseudohyperkalemia: a new twist on an old phenomenon | |
| Luppa et al. | Point-of-care testing: Principles and Clinical Applications | |
| CN102980993A (en) | Platelet aggregation function detection kit and detection method | |
| CN106645665B (en) | A kind of thrombin time detection reagent | |
| CN102692511B (en) | Kit (Developing substrate method) for testing protein C (PC) | |
| Spiezia et al. | More severe hypercoagulable state in acute COVID-19 pneumonia as compared with other pneumonia | |
| Meenakshi | Effect of dialysis on certain biochemical parameters in chronic renal failure patients | |
| Ladenson et al. | Relationship of physical symptoms, ECG, free calcium, and other blood chemistries in reinfusion with citrated blood | |
| CN103808774B (en) | Mean corpuscular concentration of glucose | |
| Lancé et al. | The effects of pneumatic tube system transport on ROTEM analysis and contact activation assessed by thrombin generation test | |
| Ergun et al. | Effects of cigarette smoking on hemorheologic parameters, plasma osmolality and lung function | |
| Salazar Vázquez et al. | Blood pressure directly correlates with blood viscosity in diabetes type 1 children but not in normals | |
| CN103926169B (en) | A kind of indirect determination method for mean corpuscular lactic acid concn | |
| Sulaiman et al. | Mitigation and detection of spurious potassium and sodium results | |
| CN105954239A (en) | Method for detecting platelet aggregation function | |
| Awodu et al. | Haemorheological variables in Nigeria pulmonary tuberculosis patients undergoing therapy | |
| CN102182116A (en) | Preparation and application method of filter paper collecting biological sample | |
| Katayama et al. | Determinants of blood rheology in healthy adults and children using the microchannel array flow analyzer | |
| CN108132284B (en) | A kind of test method of electrochemical sensor | |
| Meex et al. | Analytical validation of the new plasma calibrated Accu-Chek® test strips (Roche Diagnostics) | |
| CN103728453B (en) | A kind of vitro detection kit and detection method thereof of TAFI content | |
| Shek et al. | Errors due to heparin in the estimation of plasma sodium and potassium concentrations | |
| Monte et al. | In vitro evidence of gender-related heparin resistance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160113 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |