CN103808717A - Method for detecting mercury ions by adopting colorimetric method - Google Patents
Method for detecting mercury ions by adopting colorimetric method Download PDFInfo
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- CN103808717A CN103808717A CN201410051728.7A CN201410051728A CN103808717A CN 103808717 A CN103808717 A CN 103808717A CN 201410051728 A CN201410051728 A CN 201410051728A CN 103808717 A CN103808717 A CN 103808717A
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- folic acid
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004737 colorimetric analysis Methods 0.000 title claims abstract 8
- 229910052753 mercury Inorganic materials 0.000 title claims description 9
- -1 mercury ions Chemical class 0.000 title claims 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 239000000523 sample Substances 0.000 claims abstract description 23
- BQPIGGFYSBELGY-UHFFFAOYSA-N mercury(2+) Chemical compound [Hg+2] BQPIGGFYSBELGY-UHFFFAOYSA-N 0.000 claims abstract description 21
- 235000019152 folic acid Nutrition 0.000 claims abstract description 16
- 239000011724 folic acid Substances 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims description 26
- 238000010521 absorption reaction Methods 0.000 claims description 18
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 15
- 229960000304 folic acid Drugs 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 101710134784 Agnoprotein Proteins 0.000 claims description 3
- 229960005070 ascorbic acid Drugs 0.000 claims description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims description 3
- 239000011668 ascorbic acid Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 2
- 235000020679 tap water Nutrition 0.000 claims description 2
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000003760 magnetic stirring Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 abstract description 13
- 229910052709 silver Inorganic materials 0.000 abstract description 13
- 239000004332 silver Substances 0.000 abstract description 13
- 230000035945 sensitivity Effects 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 10
- 238000001228 spectrum Methods 0.000 abstract description 2
- 229940014144 folate Drugs 0.000 abstract 1
- 238000007306 functionalization reaction Methods 0.000 description 10
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 10
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 6
- 241000736199 Paeonia Species 0.000 description 4
- 235000006484 Paeonia officinalis Nutrition 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 238000000862 absorption spectrum Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 229940113082 thymine Drugs 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- JJWSNOOGIUMOEE-UHFFFAOYSA-N Monomethylmercury Chemical compound [Hg]C JJWSNOOGIUMOEE-UHFFFAOYSA-N 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
本发明的目的是提供一种采用比色法检测汞离子的方法，该方法采用比色法检测汞离子，叶酸银溶液在与汞离子混合1-3小时后，产生荧光发射，具有良好的灵敏度和选择性，探针灵敏度高、选择性好，检测限低。不需要大型仪器，通过裸眼观察或测试其光谱，即可识别检测结果。
The purpose of this invention is to provide a kind of method that adopts colorimetric method to detect mercury ion, and this method adopts colorimetric method to detect mercury ion, and silver folate solution produces fluorescent emission after being mixed with mercury ion for 1-3 hours, has good sensitivity and selectivity, the probe has high sensitivity, good selectivity and low detection limit. The detection result can be identified by observing or testing its spectrum with the naked eye without the need for a large instrument.
Description
Technical field
The invention belongs to nanometer detection technical field, be specifically related to the method for a kind of silver nano-grain as colorimetric and fluorescence probe detection mercury ion.
Background technology
Along with economic fast development, a large amount of undressed waste water, waste residue are discharged in rivers and lake, cause water body to be polluted.Mainly heavy metal ion through checking its pollutant, for heavy metal ion (mercury Hg
2+; Plumbous Pb
2+; Cadmium Cd
2+; Copper Cu
2+), particularly mercury, cadmium, lead, copper etc. have significant bio-toxicity.They can not be degraded by microorganisms in water body, various chemical forms can only occur and mutually transform and move.After entered environment or the ecosystem, will retain, accumulate and move, work the mischief.Wherein, mercury is ubiquitous high toxicity in environment, human health is had to an objectionable impurities of very big impact.From physiology, the steam of mercury metal and organomercurial derivant (as methyl mercury) all can produce injurious effects to a lot of aspects of the mankind's the brain physical function relevant with other.Inorganic mercury can be changed into methyl mercury by the bacterium in environment, and the mercury pollution of this form can pass to or be gathered in more higher leveled organic-biological body by food chain.And the organs such as the heart of inorganic mercury energy loss victimization class, kidney, stomach, intestines.Water miscible dimercurion (Hg
2+) be the most general and the most stable one in various forms of mercury pollution materials, it is the major way of water environment and soil pollution.Therefore, the water miscible Hg in environment
2+detection and monitoring be necessary.Traditional method for example Inductively coupled plasma method for detection of mercury ion, normally high flow rate, can not be portable, need complicated instrumental analysis.Therefore, realize real-time detection and the original position fast detecting of the mercury ion in environmental area or biosome, and reach higher sensitivity and be of great significance and considerable application prospect.
In recent years, researchist is to develop simple and effective Hg
2+detection means is target, and a collection of device with highly sensitive high selectivity is respectively based on gold, silver nano particle, fluorophor, DNA or DNA enzyme, polymeric material and protein etc.In these devices, the detection elements of many devices is thymine mispairing structures of existing in the nucleotide chain being based upon based on comprising thymine (T), utilize mispairing thymine can with Hg
2+form stable structure and detect Hg
2+[LiuCW, HsiehYT, HuangCC, LinZH, ChangHT, Chem.Comm.2008,2242 – 4; WangH, WangYX, JinJY, YangRH, Anal.Chem.2008,80,9021 – 9028; YeBC, YinBC, Angew.Chem.Int.Ed.2008,47,8386 – 9; YuCJ, ChengTL, TsengWL, BiosensorsandBioelectronics, 2009,25,204-210.].But utilizing nucleic acid or enzyme is very expensive as detection elements, and in the preparation process of device, be also complicated and consuming time, utilizing this technology is to be difficult to realize generally application.And allowing containing the high-load of inorganic mercury ion in the potable water that USEPA (EPA) issues is 2ppb (10nM).This standard is lower than the lowest detectable limit of many detection means, and many detection means do not reach.Therefore, need to design i.e. sensitive, the handled easily device of economy again to the monitoring of environment.
Silver nano-grain solution has vivid color, due to the character of the surface plasma body resonant vibration of silver nano-grain, the state variation of the size of silver nano-grain, intergranular distance and dispersion can detect by ultraviolet-visible spectrum, macro manifestations is in the change color of solution, can bore hole observe, therefore, be desirable detection means.In the present invention, we have studied a kind of method of utilizing the silver nano-grain of folic acid functionalization to detect heavy metal ion as colorimetric and fluorescence probe.And the method is by simple disposable green synthetic route, possess the low synthetic feature that is easy to of cost, and can Quantitative detection mercury ion, there is high sensitivity and selectivity.
Summary of the invention
The object of this invention is to provide a kind of method that adopts colorimetric determination mercury ion, it is characterized in that comprising the following steps:
Step 1, the nano-Ag particles (FA-AgNPs) of folic acid functionalization synthetic:
By the AgNO of the 2M of 30 μ L
3solution and 50mL tap water are mixed and heated to boiling, under the condition of magnetic agitation, add the 0.6% folic acid aqueous solution of 2mL and the aqueous ascorbic acid of 1mL0.1M to make mixed solution, the color of described mixed solution is from the colourless peony that becomes, after boiling 10min, cool to room temperature, centrifugal 30min under 10000 conditions that turn, abandoning supernatant, the centrifugal material obtaining is disperseed in 2mL water again, the nano-Ag particles (FA-AgNPs) that obtains folic acid functionalization, prepared FA-AgNPs concentration is 40～60nM;
In step 1, prepared FA-AgNPs has respectively an absorption peak at 414nm and 531nm place.
In step 2, the concentration of mercury ion solution is 1nM-50 μ M.
Described detector probe detects Hg
2+can reach 1nM.
Tool of the present invention has the following advantages:
1, colorimetric provided by the invention, fluorescent detection probe is highly sensitive, selectivity good, detectability is low.
2, do not need large-scale instrument, observe or test its spectrum by bore hole, can recognition detection result.
3, the present invention easily prepares and preserves; Under 4 ℃ of conditions, can preserve and not change for 8～15 months.
4, agents useful for same of the present invention and operating process all have no side effect.
5, the inventive method is simple, quick, easy to operate, can carry out on-the-spot original position fast detecting.
Accompanying drawing explanation
Fig. 1 is FA-AgNPs and adds 50 μ MHg
2+the uv-visible absorption spectra of the FA-AgNPs of solution;
Fig. 2 is the transmission electron microscope photo of FA-AgNPs;
Fig. 3 is for adding 50 μ MHg
2+the transmission electron microscope photo of the FA-AgNPs of solution;
Fig. 4 is the absorption ratio A that FA – AgNPs is colorimetric detection probes
531/414with Hg
2+the linear relationship of concentration (0.001-1 μ M);
Embodiment
The silver nano-grain of folic acid functionalization, by the method for colorimetric and fluoroscopic examination, can detect the micro-heavy metal ion Hg containing in aqueous sample
2+.
One, the nano-Ag particles of folic acid functionalization (FA-AgNPs) is synthetic:
By the AgNO of the 2M of 30 μ L
3solution and 50mL water are mixed and heated to boiling, under the condition of magnetic agitation, add the 0.6% folic acid aqueous solution (pH is between 11-12) of 2mL and the 0.1M aqueous ascorbic acid of 1mL, the color of mixed solution is from the colourless peony that becomes, after boiling 10min, and cool to room temperature, centrifugal 30min under 10000 conditions that turn, abandoning supernatant, disperses the centrifugal material obtaining in 2mL water again, obtains the nano-Ag particles (FA-AgNPs) of folic acid functionalization.
Prepared FA-AgNPs is carried out to ultraviolet-visible spectrum test, from accompanying drawing 1, can be observed FA-AgNPs and have respectively an absorption peak at 414nm and 531nm, be the characteristic absorption of Ag nano particle at the absorption peak at 414nm place, and be to form because FA-AgNPs assembles the surface plasma body resonant vibration absorption peak that chain causes at 531nm place.Can see by its transmission electron microscope TEM photo (accompanying drawing 2) FA-AgNPs that is chain.
Two, colorimetric determination mercury ion (Hg
2+)
When adding 50 μ MHg of equivalent in FA-AgNPs
2+after solution, test its ultraviolet-visible spectrum, as can be seen from the figure, there is blue-shifted phenomenon in the absorption peak of FA-AgNPs, and the intensity of absorption peak obviously weakens, and the variation of its absorption peak is owing to having added Hg
2+cause, this is because amino, carboxylic group and the Hg on the silver nano-grain surface of folic acid functionalization
2+between formed stable coordination structure, the existence of this structure causes the distance between silver nano-grain that variation has occurred, there is obvious state of aggregation in silver nano-grain.TEM photo Fig. 3 of sample has further proved the gathering of FA-AgNPs, has formed the stacking states of FA-AgNPs.Show that thus FA-AgNPs is to Hg
2+have certain response, these responses can be passed through ultraviolet-visible spectrum, bore hole is observed, and prove that the method is to Hg
2+detection.
1, detection sensitivity
In order to improve the sensitivity of FA-AgNPs detector probe, add NaCl solution to change the ionic strength of solution.We are to adding in FA-AgNPs the NaCl solution of 0.1-0.5M of 50-100 μ L as detector probe.The mercury ion solution of preparation variable concentrations (1nM-50 μ M) mixes with FA-AgNPs detector probe respectively.Observe its change color and carry out ultraviolet-visible spectrum test.Along with Hg
2+the increase of concentration, the color of mixed solution gradually becomes light yellow by peony, as the Hg of 50 μ M
2+after solution adds, the color of mixed solution is almost colourless transparent, and this is owing to introducing Hg
2+afterwards, make FA-AgNPs particle aggregation and the color that causes changes.It is carried out to ultraviolet-visible spectrum test, and from spectrogram, we observe, along with Hg
2+the increase of concentration, the absorption peak of FA-AgNPs engenders blue-shifted phenomenon, the intensity of absorption peak also weakens gradually.Observe with ultraviolet and detect and can show that FA-AgNPs is to Hg by bore hole
2+there is higher sensitivity.From accompanying drawing 4, can show that the silver nano-grain of folic acid functionalization is as the absorption ratio A of colorimetric detection probes
531/414with Hg
2+concentration has good linear relationship between 1nM – 1 μ M, therefore, the silver nano-grain of folic acid functionalization as colorimetric detection probes to Hg
2+detectability can reach 1nM.
2, selectivity
Under the same terms, prepare respectively the Pb of 50 μ M
2+, Cu
2+, Mg
2+, Zn
2+, Ni
2+, Co
2+, Ca
2+, Mn
2+, Fe
2+, Mg
2+, Cr
3+, Cr
6+, Cd
2+and Ba
2+as other solion, mix with FA-AgNPs detector probe respectively.Observe its change color and carry out ultraviolet-visible spectrum test.It is red that the color of the mixed solution of other ion and FA-AgNPs still keeps, and add Hg
2+the color of mixed solution become colorless by redness.Can find out that by the variation of mixed solution color FA-AgNPs is to Hg
2+selectivity.Test the ultraviolet-visible spectrum of each reaction solution, from spectrogram, we can see Hg
2+there is obvious blue-shifted phenomenon in the absorption spectrum of other Ar ion mixing solution of absorption spectrum comparison of mixed solution, and the intensity of absorption peak obviously reduces.This can further prove out that FA-AgNPs is to Hg
2+there is good selectivity.
Process sensitivity and optionally experimental study, prove that the nano-Ag particles of folic acid functionalization can be with being colorimetric detection probes detection Hg
2+, detectability can reach 1nM.
Embodiment 1:
In order to improve the sensitivity of FA-AgNPs detector probe, add NaCl solution to change the ionic strength of solution.We add the 0.1MNaCl solution of 75 μ L as detector probe in the FA-AgNPs of 5mL.The concentration of preparation mercury ion is 1nM, 10nM, 50nM, 100nM, 500nM, 1 μ M, 2 μ M, 3 μ M, 4 μ M, 5 μ M, 10 μ M, 15 μ M, 20 μ M, 50 μ M solution, mixes respectively with the FA-AgNPs detector probe of equivalent.Observe its change color and carry out ultraviolet test.From photo, can see, along with Hg
2+the increase of concentration, the color of mixed solution gradually becomes light yellow by peony, as the Hg of 50 μ M
2+after solution adds, the color of mixed solution is almost colourless transparent, and this is owing to introducing Hg
2+afterwards, FA-AgNPs is assembled and the color that causes changes.It is carried out to ultraviolet-visible spectrum test, and from spectrogram, we observe, along with Hg
2+the increase of concentration, the absorption peak of FA-AgNPs engenders blue-shifted phenomenon, the intensity of absorption peak also weakens gradually.Observe with ultraviolet and detect and can show that FA-AgNPs is to Hg by bore hole
2+there is higher sensitivity.
Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (5)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233896A (en) * | 2017-06-11 | 2017-10-10 | 哈尔滨师范大学 | A kind of silver and copper bimetal nano particle and its application |
| CN109030473A (en) * | 2018-06-13 | 2018-12-18 | 盐城工学院 | Utilize the method for Nano silver grain detection mercury ion |
| CN113960021A (en) * | 2020-07-03 | 2022-01-21 | 吉林化工学院 | Test paper for rapidly detecting mercury ions by nano silver particles based on green synthesis |
| CN115414274A (en) * | 2022-09-29 | 2022-12-02 | 宁波博雅格致健康科技有限公司 | Application of She Suanyin ionic material in preparation of scalp care composition, scalp care composition and preparation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101710076A (en) * | 2009-12-29 | 2010-05-19 | 东北师范大学 | Lead ion colorimetric detection probes and application method thereof |
| CN101713737A (en) * | 2009-12-29 | 2010-05-26 | 东北师范大学 | Fluorescent detection probe for mercury ions and application method thereof |
| CN102699343A (en) * | 2012-05-07 | 2012-10-03 | 东北师范大学 | Method for preparing gold nanoparticles |
| EP2518474A1 (en) * | 2011-04-27 | 2012-10-31 | Well Dynamics Applications S.r.l. WDA | Method for the determination of analytes in the hair |
| CN103115905A (en) * | 2013-01-27 | 2013-05-22 | 哈尔滨师范大学 | Fluorescence detection probe for lead ions |
| CN103226103A (en) * | 2013-04-04 | 2013-07-31 | 哈尔滨师范大学 | A kind of mercury ion colorimetric detection probe and application method thereof |
-
2014
- 2014-02-14 CN CN201410051728.7A patent/CN103808717B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101710076A (en) * | 2009-12-29 | 2010-05-19 | 东北师范大学 | Lead ion colorimetric detection probes and application method thereof |
| CN101713737A (en) * | 2009-12-29 | 2010-05-26 | 东北师范大学 | Fluorescent detection probe for mercury ions and application method thereof |
| EP2518474A1 (en) * | 2011-04-27 | 2012-10-31 | Well Dynamics Applications S.r.l. WDA | Method for the determination of analytes in the hair |
| CN102699343A (en) * | 2012-05-07 | 2012-10-03 | 东北师范大学 | Method for preparing gold nanoparticles |
| CN103115905A (en) * | 2013-01-27 | 2013-05-22 | 哈尔滨师范大学 | Fluorescence detection probe for lead ions |
| CN103226103A (en) * | 2013-04-04 | 2013-07-31 | 哈尔滨师范大学 | A kind of mercury ion colorimetric detection probe and application method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233896A (en) * | 2017-06-11 | 2017-10-10 | 哈尔滨师范大学 | A kind of silver and copper bimetal nano particle and its application |
| CN109030473A (en) * | 2018-06-13 | 2018-12-18 | 盐城工学院 | Utilize the method for Nano silver grain detection mercury ion |
| CN113960021A (en) * | 2020-07-03 | 2022-01-21 | 吉林化工学院 | Test paper for rapidly detecting mercury ions by nano silver particles based on green synthesis |
| CN115414274A (en) * | 2022-09-29 | 2022-12-02 | 宁波博雅格致健康科技有限公司 | Application of She Suanyin ionic material in preparation of scalp care composition, scalp care composition and preparation method |
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