CN103800318A - Application of brazilin in preparing medicaments for preventing and treating liver and kidney injury - Google Patents
Application of brazilin in preparing medicaments for preventing and treating liver and kidney injury Download PDFInfo
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- CN103800318A CN103800318A CN201410044716.1A CN201410044716A CN103800318A CN 103800318 A CN103800318 A CN 103800318A CN 201410044716 A CN201410044716 A CN 201410044716A CN 103800318 A CN103800318 A CN 103800318A
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Abstract
The invention discloses application of brazilin in preparing medicaments for preventing and treating liver and kidney injury. Brazilin can be used as a traditional Chinese medicinal component for preventing liver and kidney injury. Due to remarkable drug effect, Brazilin has potential in being developed into drugs for preventing and treating liver and kidney injury, and can be the direction in the research of new drugs for preventing and treating liver and kidney injury.
Description
Technical field
The present invention relates to the application of brazilin in the medicine for the preparation of control lesions of liver and kidney.
Background technology
Liver and kidney are that body weight for humans is wanted internal organs, its relevant disease serious threat human health.Take injury of kidney disease as example, developed country's kidney disease sickness rate is 6.5%-10%, and selected areas of China sickness rate is up to 8%-12%.From 2005, the uremic patient of China's row kidney replacement therapy approximately had 60,000 examples, and to be greater than every year 11% rate increase, became clinical common critical illness, and wherein acute injury of kidney case fatality rate is up to 30%.Therefore, to prevent and/or treat medicine significant for active development relevant disease safe and effective, with low cost.
Lignum Sappan is the dry duramen of leguminous plant Lignum Sappan (Caesalpinia sappan L.).Be distributed widely in the Guangxi of China, Guangdong, Taiwan, Guizhou, the ground such as Yunnan and Sichuan.The effect with " removing blood stasis evacuation of pus pain relieving ", brazilin (Brasilin) is its main component, structure is as follows:
Modern pharmacology research shows, brazilin all has good protection and therapeutical effect to apoplexy and myocardial ischemia, and brazilin can protect cranial nerve, increase cerebral blood flow suppresses Na-K atpase activity simultaneously, reaches cerebral protection.At present, brazilin there is no report to the curative effect of kidney and hepar damnification.
There is no at present the medicine of clear and definite control lesions of liver and kidney.Take injury of kidney as example, its therapeutic scheme comprises: the Drug therapy that 1) maintains cylinder electrolyte balance; 2) dialysis treatment; 3) kidney is replaced treatment, and due to the kidney injury treatment medicine without clear and definite, the implantation rate after injury of kidney is high.Meanwhile, due to the prevention injury of kidney medicine without clear and definite, the sickness rate of uncontrollable injury of kidney, has further increased the weight of the harm of injury of kidney to human health.
Summary of the invention
The object of the present invention is to provide the application of brazilin in the medicine for the preparation of control lesions of liver and kidney.
For achieving the above object, the present invention has adopted following technical scheme:
The application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney.
The control of injury of kidney is presented as to the improvement of serum creatinine.
The control of injury of kidney is presented as to the improvement of blood urea nitrogen.
The control of hepatic injury is presented as to the improvement of alanine aminotransferase.
The control of hepatic injury is presented as to the improvement of aspartate aminotransferase.
The dosage form of described medicine comprises the multiple interior external preparation such as powder, pill, capsule, tablet, microcapsule, soft microcapsule, membrane, suppository, injection, unguentum, tincture, powder, electuary or aerosol.
Beneficial effect of the present invention is embodied in:
1) brazilin of the present invention belongs to Chinese medicine ingredients, and toxic and side effects is low;
2) the present invention can effectively improve hepar damnification, maintains and repair liver function;
3) the present invention can effectively improve kidney injury, maintains and repair renal function;
4) brazilin of the present invention has exploitation becomes the potential quality of Liver and kidney protection medicine, or as basis/prodrug, for development of new Liver and kidney protection medicine, is new thinking and the research structure of Liver and kidney protection drug provision.
Accompanying drawing explanation
Fig. 1 is the variation of liver organization pathological section after rat different disposal, A: sham operated rats pathological section figure; B: model group pathological section figure; C: brazilin administration group pathological section figure;
Fig. 2 is Scr(serum creatinine after rat different disposal) variation (
), * represents P ﹤ 0.05 compared with sham operated rats, # represents P ﹤ 0.05 compared with model group;
Fig. 3 is BUN(blood urea nitrogen after rat different disposal) variation (
), * represents P ﹤ 0.05 compared with sham operated rats, # represents P ﹤ 0.05 compared with model group;
Fig. 4 is the variation of kidney tissue pathological slice after rat different disposal, sham: sham operated rats pathological section figure; I/R: model group pathological section figure; I/R+Bra: brazilin administration group pathological section figure;
Fig. 5 be TNF-α (tumor necrosis factor-alpha) after rat different disposal variation (
), * represents P ﹤ 0.05 compared with sham operated rats, # represents P ﹤ 0.05 compared with model group;
Fig. 6 is caspase3(glutathion desmoenzyme-3 after rat different disposal) variation (
), * represents P ﹤ 0.05 compared with sham operated rats, # represents P ﹤ 0.05 compared with model group.
The specific embodiment
Below in conjunction with drawings and Examples, the present invention is elaborated.
In embodiment, brazilin is purchased from Shanghai Kemin Biological Technology Co., Ltd..
Embodiment 1: the protective effect of brazilin to rats'liver ischemia/reperfusion (LI/R) damage
30 of SD rats, body weight 220-280g, purchased from The Fourth Military Medical University's zoopery center.Rat sub-cage rearing (ambient temperature is 24 ℃, and humidity is 60-70%).30 rats are divided into following several groups (n=6) at random: sham operated rats, model group and brazilin administration group (containing high, medium and low 3 dosage groups), preoperative 6d fasting, freely drink water.Each group rat is through 10% chloral hydrate intraperitoneal injection of anesthesia, then brazilin administration group is carried out the (clarification of rat tail vein injection brazilin aqueous solution, after filtration), brazilin is respectively by high, normal, basic 3 dosage levels (120,60,30mg/kg) administration, sham operated rats and model group injecting normal saline.
30min after administration, respectively organizes rat eye socket blood sampling 1.0mL; Then by prostrate rat fixing, sham operated rats is sewed up after opening abdomen; Brazilin administration group and model group are opened abdomen, and ligation Hepatic artery, portal vein and bile duct in the lump, unclamps after ligation 30min, then carry out two-layered suture.Postoperative 4h, 10h respectively organize rat and carry out eye socket blood sampling (about 1.0mL); Postoperative 24h respectively organizes rat and gets liver organization, and blood sample collection 1.5mL, then gets hematometry alanine aminotransferase (ALT) and aspartate aminotransferase (AST) while pouring into 4,10,24h.
The results are shown in Table 1 and Fig. 1, in brazilin administration group, high, normal, basic 3 dosage waters on average show liver protective effect significantly, and have concentration dependent; Pathological section shows simultaneously: the tissue necrosis area after brazilin administration (30mg/Kg) is significantly less than model group, and inflammation damnification is also significantly lower than model group simultaneously.
The variation of table 1 each group rat blood serum ALT and AST (
)
Note: sham: sham operated rats; LI/R: model group; High dose, middle dosage, low dosage: brazilin dosage is respectively 120mg/kg, 60mg/kg, 30mg/kg.* represent to there is significant difference compared with sham, P ﹤ 0.05; # represents to have significant difference compared with LI/R, P ﹤ 0.05.
Embodiment 2: the protective effect of brazilin to kidney of rats ischemia/reperfusion (RI/R) damage
30 of SD rats, body weight 220-280g, purchased from The Fourth Military Medical University's zoopery center.(ambient temperature is 24 ℃ to rat sub-cage rearing, humidity is 60-70%), 30 rats are divided into following several groups (n=6) at random: sham operated rats, model group and brazilin administration group (containing high, medium and low 3 dosage groups), preoperative 6d fasting, freely drink water.Each group rat is sleeping fixing through 10% chloral hydrate intraperitoneal injection of anesthesia, the back of the body, then brazilin administration group is carried out the (clarification of rat tail vein injection brazilin aqueous solution, after filtration), brazilin is respectively by high, normal, basic 3 dosage levels (120,60,30mg/kg) administration, sham operated rats and model group injecting normal saline.
30min after administration, respectively organizes rat eye socket blood sampling 1.0mL, then carries out Resection of right kidney art: the right kidney of rat back place otch, find out after right kidney separates and excise; After excision, carry out two-layered suture; Brazilin administration group separates the left renal aorta operation of ligation with model group: the left kidney otch from back, find out left kidney, and separate left renal aorta, after the left renal aorta 30min of ligation, unclamp, then carry out two-layered suture.Postoperative 4h, 10h respectively organize rat and carry out eye socket blood sampling (about 1.0mL); Postoperative 24h respectively organizes rat and wins left kidney, and blood sample collection 1.5mL.Left kidney is equally divided into two halves and preserves, and half is made tissue slice, and pathological section is done in half censorship.
The results are shown in Figure 2-Fig. 4, in brazilin administration group, high, normal, basic 3 dosage waters on average show kidney protective effect significantly, have concentration dependent.The BUN that pours into again 24h under high dose level obviously declines, the Scr sham operated rats of being on close level.Pathological section shows simultaneously: the tissue necrosis area after brazilin administration (30mg/Kg) is significantly less than model group, and inflammation damnification is also significantly lower than model group simultaneously.From Fig. 5 and Fig. 6, brazilin administration (30mg/Kg) can significantly reduce TNF-α and caspase3, infers thus, and brazilin control ischemia/reperfusion injury of kidney may, by reducing inflammatory damage, reduce apoptosis and realize.
Brazilin is the Chinese medicine ingredients that can be used for preventing liver kidney injury, and its significant drug effect makes it have the potential that is developed to lesions of liver and kidney control medicine, also can become the research direction of lesions of liver and kidney control new drug.
Claims (6)
1. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney.
2. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney according to claim 1, is characterized in that: the control of injury of kidney is presented as to the improvement of serum creatinine.
3. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney according to claim 1, is characterized in that: the control of injury of kidney is presented as to the improvement of blood urea nitrogen.
4. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney according to claim 1, is characterized in that: the control of hepatic injury is presented as to the improvement of alanine aminotransferase.
5. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney according to claim 1, is characterized in that: the control of hepatic injury is presented as to the improvement of aspartate aminotransferase.
6. the application of brazilin in the medicine for the preparation of control hepatic injury or injury of kidney according to claim 1, is characterized in that: the dosage form of described medicine comprises powder, pill, capsule, tablet, microcapsule, soft microcapsule, membrane, suppository, injection, unguentum, tincture, powder, electuary or aerosol.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112353834A (en) * | 2020-12-03 | 2021-02-12 | 中国人民解放军空军军医大学 | Application of two-component Shensu drink in preparation of medicine for preventing and treating chronic kidney injury |
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2014
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Non-Patent Citations (3)
| Title |
|---|
| MOON C.K. 等: "Brazilin protects cultured rat hepatocytes from bromochloroform-induced toxicity", 《DRUG CHEM TOXICAL》, vol. 15, no. 1, 31 December 1992 (1992-12-31), pages 81 - 91 * |
| 张红等: "苏木水煎液对糖尿病佘冰大鼠血糖、肾功能和肾脏病理影响", 《时珍国医国药》, vol. 22, no. 5, 31 December 2011 (2011-12-31), pages 1255 - 1256 * |
| 陈雪敏等: "高效液相色谱法同时测定苏木中的巴西苏木素和原苏木素B", 《分析试验室》, vol. 31, no. 4, 30 April 2012 (2012-04-30), pages 98 - 101 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112353834A (en) * | 2020-12-03 | 2021-02-12 | 中国人民解放军空军军医大学 | Application of two-component Shensu drink in preparation of medicine for preventing and treating chronic kidney injury |
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