CN103787480A - Waste liquid treatment method of gram stain two-step method - Google Patents
Waste liquid treatment method of gram stain two-step method Download PDFInfo
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- CN103787480A CN103787480A CN201210418360.4A CN201210418360A CN103787480A CN 103787480 A CN103787480 A CN 103787480A CN 201210418360 A CN201210418360 A CN 201210418360A CN 103787480 A CN103787480 A CN 103787480A
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- waste liquid
- stain
- trichloroisocyanuric acid
- aqueous solution
- counterstain
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Abstract
The present invention discloses a waste liquid treatment method of a gram stain two-step method. The waste liquid treatment method comprises: adding a trichloroisocyanuric acid aqueous solution or a flaky trichloroisocyanuric acid solid to a collected primary stain and counter staining waste liquid to produce precipitate, and filtering, wherein the primary stain agent is a crystal violet-containing aqueous solution, the counter staining agent is an ethanol solution containing basic fuchsin, potassium iodide and iodine, a volume ratio of the waste liquid to the trichloroisocyanuric acid aqueous solution is 10:1-30:1, and the feeding amount of the flaky trichloroisocyanuric acid solid in per 100 mL of the waste liquid is 0.03-0.1 g. With the method, the waste liquid is effectively treated, such that the treated waste liquid achieves the waste water emission standard, and the environment pollution is reduced.
Description
Technical field
The present invention relates to a kind of method for treating waste liquid, particularly relate to a kind of method for treating waste liquid of gram stain two-step method.
Background technology
Gram staining is widely used a kind of differential staining in bacteriology, within 1884, is founded by Denmark doctor Gram.Being differential staining very important in bacteriology, because dye by this method, can be gram positive organism (G+) and the large class of gram-negative bacteria (G-) two by discrimination of bacteria.Traditional gram staining method generally comprises just dyes, mordant dyeing, four steps such as decolours, redyes, wherein just stain is the ammonium oxalate crystal violet aqueous solution, mordant is the aqueous solution of iodine, counterstain is luxuriant red colouring liquid, traditional gram staining method is concentrated discharge after conventionally the waste liquid that contains dyestuff producing after dyeing being placed in to waste liquid cylinder, described waste liquid is not taked to effective treatment process, therefore environment causes larger pollution.
In order to simplify staining procedure, reduce the consumption of dyestuff, reduce environmental pollution, at present traditional gram's staining four step rule is also done to many improvement, wherein the most simple and effective is exactly to wash the gram stain two-step method of twice, the method mainly comprises just dyes and redyes two steps, and wherein said just stain is the aqueous solution that contains Viola crystallina, and described counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine.Although the method is by simplifying step compared with four step rule, reduce dye dosage, reduce to a certain extent the pollution of environment, the waste liquid that contains first stain and counterstain that the method is not mentioned producing carries out effective treatment process, therefore the waste liquid producing does not still reach standard that can safety dumping, and environment is equally easily caused to larger pollution.
Summary of the invention
The object of the invention is for there is no effective treatment process for the waste liquid producing in gram stain two-step method at present, easily cause the problem of environmental pollution, a kind of method for treating waste liquid of gram stain two-step method is provided, the method has carried out effectively processing for the rear waste liquid producing of dyeing, waste liquid after treatment is reached can emission standard, has reduced the pollution to environment.
Object of the present invention can be achieved through the following technical solutions:
A method for treating waste liquid for gram stain two-step method, comprises the following steps: in the waste liquid of the first stain of collecting and counterstain, add the trichloroisocyanuric acid aqueous solution or sheet trichloroisocyanuric acid solid, after generation precipitate, filter;
Wherein said just stain is the aqueous solution that contains Viola crystallina, described counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, the volume ratio of described waste liquid and the trichloroisocyanuric acid aqueous solution is 10:1～30:1, described in every 100mL, in waste liquid, the charging capacity of sheet trichloroisocyanuric acid solid is 0.03g～0.1g.
Present method is by adding the trichloroisocyanuric acid aqueous solution or sheet trichloroisocyanuric acid solid in the waste liquid to containing the first stain that inclined and counterstain, after leaving standstill, filtering generation precipitate realizes, present method can make first stain and counterstain mixing stain and the trichloroisocyanuric acid generation complex compound in waste liquid and separate out, and makes sewage after treatment hospital sewage discharge up to standard national standard.
The present invention preferably, the trichloroisocyanuric acid that the described trichloroisocyanuric acid aqueous solution contains mass percent 1.0%～3.0%.
Preferably, the time of separating out of described precipitate is 5～24 hours in the present invention.
Preferably, in the described waste liquid that contains the first stain that inclined and counterstain, the volume ratio of first stain and counterstain is 1:2～3:0.8, more preferably 2:1 in the present invention.
The method for treating waste liquid of gram stain two-step method of the present invention has the following advantages:
In the waste liquid of present method by the first stain to containing collection and counterstain, add the trichloroisocyanuric acid aqueous solution or sheet trichloroisocyanuric acid solid, generation complex compound is separated out the step of rear filtration waste liquid has been carried out effectively processing, waste liquid after treatment has been reached can emission standard, has reduced the pollution to environment.
Embodiment
To the technical scheme in the embodiment of the present invention be clearly and completely described below, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
In the present invention, use just stain, counterstain and trichloroisocyanuric acid to be conventional material, the filter method using in the present invention is the method for conventional use gauze and cotton filtration.
Embodiment 1
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 2:1; In described waste liquid, add the trichloroisocyanuric acid aqueous solution 10ml that contains mass percent 1.0%, leave standstill after 5 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
Embodiment 2
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 1:2; In described waste liquid, add the trichloroisocyanuric acid aqueous solution 3.3ml that contains mass percent 3.0%, leave standstill after 24 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
Embodiment 3
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 3:0.8; In described waste liquid, add the trichloroisocyanuric acid aqueous solution 5ml that contains mass percent 2.0%, leave standstill after 24 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
Embodiment 4
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 1:2; In described waste liquid, add 0.1g sheet trichloroisocyanuric acid solid, leave standstill after 5 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
Embodiment 5
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 1:1; In described waste liquid, add 0.06g sheet trichloroisocyanuric acid solid, leave standstill after 24 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
Embodiment 6
Collection contains in gram stain two-step method the first stain that inclined and the waste liquid 100ml of counterstain, wherein just stain is the aqueous solution that contains Viola crystallina, counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, and the volume ratio of first stain and counterstain is 3:1; In described waste liquid, add 0.03g sheet trichloroisocyanuric acid solid, leave standstill after 24 hours, generate complex compound and separate out, filter, water sample after treatment reaches the emission standard of quality examination unit.
The above; only for the better embodiment of the present invention, but protection scope of the present invention do not limit to therewith, is anyly familiar with in technical scope that those skilled in the art disclose in the present invention; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.
Claims (4)
1. a method for treating waste liquid for gram stain two-step method, comprises the following steps:
In the first stain inclining to quilt and the waste liquid of counterstain, add the trichloroisocyanuric acid aqueous solution or sheet trichloroisocyanuric acid solid, after generation precipitate, filter;
Wherein said just stain is the aqueous solution that contains Viola crystallina, described counterstain is the ethanolic soln that contains magenta, potassiumiodide, iodine, the volume ratio of described waste liquid and the trichloroisocyanuric acid aqueous solution is 10:1～30:1, described in every 100mL, in waste liquid, the charging capacity of sheet trichloroisocyanuric acid solid is 0.03g～0.1g.
2. the method for treating waste liquid of gram stain two-step method as claimed in claim 1, is characterized in that, the trichloroisocyanuric acid that the described trichloroisocyanuric acid aqueous solution contains mass percent 1.0%～3.0%.
3. the method for treating waste liquid of gram stain two-step method as claimed in claim 1, is characterized in that, the time of separating out of described precipitate is 5～24 hours.
4. the method for treating waste liquid of gram stain two-step method as claimed in claim 1, is characterized in that, in the described waste liquid that contains the first stain that inclined and counterstain, the volume ratio of first stain and counterstain is 1:2～3:0.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210418360.4A CN103787480A (en) | 2012-10-26 | 2012-10-26 | Waste liquid treatment method of gram stain two-step method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210418360.4A CN103787480A (en) | 2012-10-26 | 2012-10-26 | Waste liquid treatment method of gram stain two-step method |
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| CN103787480A true CN103787480A (en) | 2014-05-14 |
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| CN201210418360.4A Pending CN103787480A (en) | 2012-10-26 | 2012-10-26 | Waste liquid treatment method of gram stain two-step method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56124490A (en) * | 1980-03-06 | 1981-09-30 | Nissan Chem Ind Ltd | Purification of waste water in dyeing process |
| CN87102687A (en) * | 1982-10-22 | 1988-10-26 | 克莱斯塔尔克莱公司Sa | The method of water treatment and product |
| CN1623923A (en) * | 2003-12-02 | 2005-06-08 | 中国科学院生态环境研究中心 | Compound water treatment oxidation decolour agent |
| CN101293689A (en) * | 2007-08-15 | 2008-10-29 | 王旗 | Water treatment medicament |
-
2012
- 2012-10-26 CN CN201210418360.4A patent/CN103787480A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56124490A (en) * | 1980-03-06 | 1981-09-30 | Nissan Chem Ind Ltd | Purification of waste water in dyeing process |
| CN87102687A (en) * | 1982-10-22 | 1988-10-26 | 克莱斯塔尔克莱公司Sa | The method of water treatment and product |
| CN1623923A (en) * | 2003-12-02 | 2005-06-08 | 中国科学院生态环境研究中心 | Compound water treatment oxidation decolour agent |
| CN101293689A (en) * | 2007-08-15 | 2008-10-29 | 王旗 | Water treatment medicament |
Non-Patent Citations (1)
| Title |
|---|
| 许静: ""氰尿酸及其氯代衍生物的合成与研究"", 《中国优秀硕士学位论文全文数据库》, 7 July 2003 (2003-07-07) * |
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Effective date of abandoning: 20161019 |
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