CN103781897B - Filling device and fill method - Google Patents
Filling device and fill method Download PDFInfo
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- CN103781897B CN103781897B CN201280043809.1A CN201280043809A CN103781897B CN 103781897 B CN103781897 B CN 103781897B CN 201280043809 A CN201280043809 A CN 201280043809A CN 103781897 B CN103781897 B CN 103781897B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B3/00—Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans, or jars
- B65B3/26—Methods or devices for controlling the quantity of the material fed or filled
- B65B3/30—Methods or devices for controlling the quantity of the material fed or filled by volumetric measurement
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The present invention relates to a kind of fill method, it has following filling work procedure, valve (2) by the front end of the movable sprinklers that can move up and down (3) of filling device (10) is configured in container (30), the liquid of at least one party the thalline and the handled thing thereof that contain microorganism of carrying from storagetank (20) from described valve (2) discharge, in described filling work procedure, make at least one party in the flow at the initial stage of filling and the flow in filling later stage be less than maximum stream flow; Or relate to following fill method, at the filling initial stage of described filling work procedure, the valve (2) of the front end of movable sprinklers (3) is configured near the bottom surface of container (30), and then, the filling situation according to liquid in container (30) rises movable sprinklers (3). According to the present invention, can provide can by the efficient liquid of at least one party in the thalline that contains microorganism and handled thing thereof be filled into filling device and the fill method in container.
Description
Technical field
The present invention relates to stockpile the thalline that contains microorganism in storagetank and/or the liquid of its handled thingBe filled into filling device and fill method in container.
The application is based on advocating for No. 2011-196036 in the Japanese Patent Application of Japanese publication on September 8th, 2011Priority, and its content is applied to this.
Background technology
In recent years, there is energy-conservation, saving resource from effectively utilizing the production of utility etc., low public hazardsThe viewpoint consideration of the biochemical reaction of the features such as technique, wish utilizes the trend of organism catalyst prevailing gradually. DoFor organism catalyst, use zooblast, plant cell, the cell of the enzyme that contains catalysis goal response littleThe thalline of organ, microorganism, their handled thing etc. As an example of such enzyme, can enumerate and haveThe nitrile hydratase of the hydratability of itrile group, have itrile group hydrolysis ability nitrilase or there is acid amidesThe amidase of the hydrolysis ability of base etc. The utilization of these enzymes can realize acid amides important on chemical industry orThe low price manufacture of person's carboxylic acid etc.
In recent years, due to the popularization that the organism catalyst in industrial technology field utilizes, not only anti-for enzymeThe reactive mode of answering, and propose for the peripheral techniques such as store method, fill method of organism catalystImprovement or scheme. For example, in patent documentation 1, as contain for pasteurization microorganism before treatment,For example contain the thalline of microorganism of nitrile hydratase or the store method of the liquid of bacterial disposing thing, proposedThe pH of described liquid is adjusted into 6～8 methods of ventilating and stirring on one side on one side.
Prior art document
Patent documentation
Patent documentation 1: No. 4205332 communique of Japan Patent
Summary of the invention
The problem that invention will solve
The thalline that contains microorganism and/or the liquid of its handled thing easily foam. Therefore, there are the following problems:At time foaming from the storage tank of preserving described liquid is filled into filling containers, the filling rate of container per unit volumeReduce.
The present invention In view of the foregoing completes, its object be to provide can be by the thalline that contains microorganism and/ or the efficient liquid of its handled thing be filled into filling device and the fill method in container.
Solve the method for problem
The present invention who solves above-mentioned problem has following scheme.
[1] filling device, it is by the thalline that contains microorganism and/or its place that stockpile in storagetankThe liquid filling of reason thing is to the filling device in container,
Described filling device possesses nozzle, and this nozzle enters the inside of described container, will be defeated from described storagetankThe described liquid sending is discharged in described container, makes the liquid discharge portion of described nozzle from the filling of described liquidStart to be arranged in filling liquid midway.
[2] filling device, it is by the thalline that contains microorganism and/or its place that stockpile in storagetankThe liquid filling of reason thing is to the filling device in container,
Described filling device possesses: nozzle, by the described liquid of carrying from described storagetank in described containerDischarge; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit,Filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge.
[3] filling device, it is by the thalline that contains microorganism and/or its place that stockpile in storagetankThe liquid filling of reason thing is to the filling device in container,
Described filling device possesses: the movable sprinklers that can move up and down, and by what carry from described storagetankDescribed liquid is discharged in described container; Detecting unit, detects the filling shape of described liquid in described containerCondition; And nozzle location control module, the filling situation according to described liquid in described container, controls instituteState the position of the front end of movable sprinklers.
[4] according to the filling device described in any one in claim 1～3, be configured to: described storagetank positionIn the top of this filling device, utilize gravity by described liquid filling in described container.
[5] according to the filling device described in any one in claim 1～4, the thalline of described microorganism and/Or its handled thing has nitrile hydratase activity.
[6] fill method, it is by the thalline that contains microorganism and/or its place that stockpile in storagetankThe liquid filling of reason thing is to the fill method in container,
Described fill method has the front-end configuration of the nozzle of filling device in described container, from describedEnd is discharged the filling work procedure of described liquid, and described filling device possesses: nozzle, will carry from described storagetankDescribed liquid in described container, discharge; Detecting unit, detects the filling of described liquid in described containerSituation; And flow controlling unit, the filling situation according to described liquid in described container, controls from instituteState the flow of the liquid of nozzle discharge,
Described filling work procedure comprises to be made the flow at the initial stage of filling and/or fills the flow in later stage to be less than max-flowAmount.
[7] fill method according to claim 6, the flow at the filling initial stage of described filling work procedure isBelow 1/2 of maximum stream flow.
[8] according to the fill method described in claim 6 or 7, the stream in the filling later stage of described filling work procedureAmount is below 1/2 of maximum stream flow.
[9] fill method, it is by the thalline that contains microorganism and/or its place that stockpile in storagetankThe liquid filling of reason thing is to the fill method in container,
Described fill method has the front-end configuration of the movable sprinklers of filling device in described container, fromDescribed front end is discharged the filling work procedure of described liquid, and described filling device possesses: what can move up and down movesMoving nozzle is discharged the described liquid of carrying from described storagetank in described container; Detecting unit, detectsThe filling situation of described liquid in described container; And nozzle location control module, exist according to described liquidFilling situation in described container, controls the position of the front end of described movable sprinklers,
At the filling initial stage of described filling work procedure, by the front-end configuration of described movable sprinklers at described containerNear bottom surface, then, the filling situation according to described liquid in described container makes on described movable sprinklersRise.
[10] according to the fill method described in any one in claim 6～9, the thalline of described microorganism and/Or its handled thing has nitrile hydratase activity.
, the present invention relates to following content.
(1) filling device, it is to stockpile the thalline that contains microorganism in storagetank and to locateAt least one party of reason in thing liquid filling is to the filling device in container, and described filling device possesses nozzle,This nozzle enters the inside of described container, and the described liquid of carrying from described storagetank is arranged in described containerGo out, make the liquid discharge portion of described nozzle start to be arranged in filling liquid midway from the filling of described liquid;
(2) filling device, it is to stockpile the thalline that contains microorganism in storagetank and to locateAt least one party of reason in thing liquid filling is to the filling device in container, and described filling device possesses: nozzle,The described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects described liquidFilling situation in described container; And flow controlling unit, according to described liquid in described containerFilling situation, controls from the flow of the liquid of described nozzle discharge;
(3) filling device, it is to stockpile the thalline that contains microorganism in storagetank and to locateAt least one party of reason in thing liquid filling is to the filling device in container, and described filling device possesses: canThe movable sprinklers moving up and down is discharged the described liquid of carrying from described storagetank in described container;Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control module,Filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers;
(4) according to the filling device described in any one in (1)～(3), be configured to: described storagetankBe positioned at the top of this filling device, utilize gravity by described liquid filling in described container;
(5) according to the filling device described in any one in (1)～(4), the thalline of described microorganism andAt least one party in its handled thing has nitrile hydratase activity;
(6) fill method, it is to stockpile the thalline that contains microorganism in storagetank and to locateAt least one party of reason in thing liquid filling is to the fill method in container, and described fill method has fillingThe front-end configuration of the nozzle of device, in described container, is discharged the filling work procedure of described liquid from described front end,Described filling device possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container;Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, according toThe filling situation of described liquid in described container, controls from the flow of the liquid of described nozzle discharge, described inFilling work procedure comprises that at least one party in the flow that makes the flow at the initial stage of filling and fill the later stage is less than max-flowAmount;
(7), according to the fill method (6) described, the flow at the filling initial stage of described filling work procedure is maximumBelow 1/2 of flow;
(8) according to claim (6) or (7) described fill method, the filling of described filling work procedureThe flow in later stage is below 1/2 of maximum stream flow;
(9) fill method, it is to stockpile the thalline that contains microorganism in storagetank and to locateAt least one party of reason in thing liquid filling is to the fill method in container, and described fill method has fillingThe front-end configuration of the movable sprinklers of device, in described container, is discharged the filling of described liquid from described front endOperation, described filling device possesses: the movable sprinklers that can move up and down, will carry from described storagetankDescribed liquid in described container, discharge; Detecting unit, detects the filling of described liquid in described containerSituation; And nozzle location control module, the filling situation according to described liquid in described container, controlsThe position of the front end of described movable sprinklers, at the filling initial stage of described filling work procedure, by described removable sprayThe front-end configuration of mouth near the bottom surface of described container, then, the filling out in described container according to described liquidThe situation of filling rises described movable sprinklers; And
(10) according to the fill method described in any one in (6)～(9), the thalline of described microorganism andAt least one party in its handled thing has nitrile hydratase activity.
Invention effect
According to the present invention, can provide can be by least one party in the thalline that contains microorganism and handled thing thereofEfficient liquid be filled into filling device and the fill method in container.
Brief description of the drawings
Fig. 1 is the skeleton diagram that represents the formation of the filling device 10 using in embodiment 1.
Detailed description of the invention
Filling device of the present invention and fill method are by the bacterium that contains microorganism stockpiling in storagetankThe liquid filling of at least one party in body and handled thing thereof is to filling device and fill method in container.
At this, the thalline of the microorganism using in the present invention can be that viable bacteria body can be also dead thalline.
As the handled thing of thalline, can enumerate the crushed material of thalline (viable bacteria body or dead thalline), from bacteriumThe extract of body, thalline or extract are fixed on to fixed compound of carrier etc. As described extract,Preferred enzyme (rough enzyme or refining enzyme).
As the method for fixing thalline or extract, can enumerate investment, cross-linking method, or carrier combinationMethod etc. So-called investment is that thalline or enzyme are wrapped in the small lattice of high-molecular gel or utilizedThe coated method of macromolecule epithelium of pellicle. So-called cross-linking method, be with have 2 or 2 withOn the reagent (multi-functional crosslinking agent) of functional group by method crosslinked enzyme. So-called carrier combined techniques,To make enzyme be incorporated into the method for non aqueous carrier.
As the carrier using in immobilization, can enumerate for example bead, silica dioxide gel, poly-ammoniaEster, polyacrylamide, polyvinyl alcohol, carragheen, alginic acid, agar or gelatin etc.
As at least one party in thalline and the handled thing thereof of microorganism, preferably have and can urge as organismThe enzymatic activity that agent is utilized. As described enzymatic activity, can enumerate for example nitrile hydratase activity, nitrilaseActivity or lactamase activity etc. In the middle of these, preferably nitrile hydratase activity.
So-called nitrile hydratase activity, refers to that the itrile group that acts on nitrile compounds makes it be converted to corresponding acylThe activity of amines. As the assay method of nitrile hydratase activity, can enumerate and for example be determined at reaction temperatureWhile reacting 10 minutes at spending 10 DEG C, every 1mg is dried the method for the reaction speed of thalline etc.
Of the present inventionly at least one in the thalline that contains microorganism in storagetank and handled thing thereof will be stockpiledThe liquid filling of side, to the fill method in container, preferably uses the nitrile hydratase with 50～500U/mgAt least one party in thalline and the handled thing thereof of active microorganism.
As at least one party who has in thalline and the handled thing thereof of microorganism of nitrile hydratase activity, Ke YijuThe nitrile hydratase that goes out to contain the thalline of the microorganism of nitrile hydratase, extract from described thalline, by described thalline orThe immobilized fixed compound of person's nitrile hydratase etc.
As the microorganism that contains nitrile hydratase, as long as thering is the catalyst activity that nitrile is converted to amide-typeProperty (nitrile hydratase activity) microorganism just all can use. Can enumerate and for example belong to bacillus(Bacillus) genus, sporeless bacterium (Bacterinium) belong to, micrococcus luteus (MicrOcOccus) belongs to,Brevibacterium (Brevibacterium) belongs to, corynebacteria (Corynebacterium) belongs to, promise card bacterium (Nocardia)Genus, pseudomonad (Pseudomonas) belong to, microbacterium (Microbacterium) belongs to, red coccus(Rhodococcus) genus, Gordon Salmonella (Gordona) belong to, vibrios (Vibrio) belongs to, Nitrosomas(Nitrosomonas) genus, streptococcus (Streptococcus) belong to, lactobacillus (Lactobacillus) belongs to,Nitrogen-fixing bacteria (Attotobacter) belong to, saccharomycete (Saccharomyces) belongs to, endomyces (Endomyces)Genus, aspergillus (Aspergillus) belong to, mould (Penicillium) belongs to, Mucor (Mucor) belongs to, head mold(Rhizopus) genus, achromobacter (Achromobacter) genus, Selective medium (Pscudonocardia)The microorganism of genus, sickle-like bacteria (Fusarium) genus or agrobacterium (Agrobacterium) genus etc.,Preferred red coccus (Rhodococcus) belongs to.
In addition, also can use the form quality conversion body obtaining as follows: obtain the nitrile hydration from described microorganismThe target gene of enzyme, nitrilase or amidase etc., utilizes conventional method, by described gene directly,Or improve artificially, described gene is imported to any host (with reference to MolecularCloning2ndEdition, ColdSpringHaborLaboratoryPress, 1989). As such form quality conversion body,Can enumerate the nitrile hydratase of for example using achromobacter (Achromobacter) to belong to bacterium and carry out form quality conversionEscherichia coli MT10770(FERMP-14756) (with reference to Japanese kokai publication hei 8-266277 communique),The Escherichia coli that carry out form quality conversion with the nitrile hydratase of Selective medium (Pseudonocardia) genus bacteriumMT10822(FERMBP-5785) (with reference to Japanese kokai publication hei 9-275978 communique) or with purplish redThe nitrile hydratase that red coccus (Rhodococcusrhodochrous) is planted is (with reference to Japanese kokai publication hei 4-211379Number communique) carry out microorganism of form quality conversion etc.
Described microorganism can be used separately any a kind, also two or more can be used in combination.
As the liquid of at least one party in the thalline that contains microorganism and handled thing thereof, can enumerate micro-lifeThe nutrient solution that thing is cultivated with fluid nutrient medium; Separate from nutrient solution, the resting thalli cleaning as required suspendsLiquid; Or at low temperatures, resting thalli suspension is pulverized with ultrasonic wave or metal microballon etc.Thalline pulverize liquid etc.
Condition of culture (composition, pH or the cultivation temperature etc. of fluid nutrient medium) can adopt for instituteThe known condition of culture of the microorganism of cultivating. For example there is the situation of microorganism of nitrile hydratase activityUnder, added carbon source (carbohydrate such as glucose or fructose), nitrogenous source (ammonium sulfate, ammonium chloride orPerson's ammonium nitrate etc. are inorganic nitrogen-sourced; The organic nitrogen sources such as yeast extract, peptone or meat extract) andIn the fluid nutrient medium of inorganic salts, slaine or vitamin etc. as required, with 20～40 DEG C,Cultivate pH5～9. Cultivation can suitably adopt shaken cultivation or stir culture.
The liquid of at least one party in the thalline and the handled thing thereof that contain microorganism obtaining is as described above logicalOften be stored in storagetank, then, be filled into the container for carrying or preserve described liquid.
Storagetank preserving described liquid, industrial being applicable to from manufacturing during till in being filled into containerUse metal, can be by the temperature control of the liquid stockpiling be suitable for preserve temperature (for example 5 DEG C withUnder) storagetank.
Container, as long as the container that is suitable for filling liquid is just not particularly limited, can utilize at present in order to removeThe liquid of at least one party in thalline and handled thing thereof that fortune or storage contain microorganism and the container that uses. OneAs, can use metal system or resinous cylinder or tank etc. As cylinder, conventionally can useInternal volume is the cylinder of 200L left and right. As tank, conventionally can use internal volume is 1～10m3Left and rightTank.
When filling device of the present invention and fill method can be filled from this storagetank to described containerUse.
Filling device of the present invention possesses nozzle, and this nozzle enters the inside of container, by what carry from storagetankLiquid is discharged in container, makes the liquid discharge portion of described nozzle start to be positioned at from the filling of described liquid midwayIn filling liquid.
Described filling device preferably possesses according to making the liquid discharge portion of described nozzle from the filling of described liquidStart to be arranged in the control device that the mode of filling liquid is controlled midway.
In filling device of the present invention, the liquid of carrying from storagetank is entered to appearance to the nozzle of discharging in containerDevice inside, refers to the inner side that the liquid discharge portion of nozzle is inserted into upper cover from the upper cover of container.
In addition, make the liquid discharge portion of nozzle start to be arranged in filling liquid midway from filling, refer at the beginning of fillingPhase, owing to there is no filling liquid, is therefore impossible, but when becoming filling liquid and stockpile the state in containerStart, form the mode that is arranged in liquid with the liquid discharge portion of nozzle nozzle is inserted into the shape of liquidState. , refer to that the liquid discharge portion of nozzle is arranged in the filling liquid of described container.
By carry out the liquid filling to container under such state, can be suppressed at from nozzle and discharge liquidTime air be involved in. Can suppress thus foaming, the charging efficiency of container per unit volume improves.
Filling device of the present invention preferably also possesses to liquid the filling in container except described nozzleThe detecting unit that situation detects and according to liquid the filling situation in container control from nozzle and arrangeThe flow controlling unit of the flow of the liquid going out.
As the filling situation in container, can enumerate loading (liquid weight of filling), filling rate (is filled outThe volume of the liquid filling is with respect to the ratio of the internal volume of container) or the filling stage etc.
As the detecting unit of the filling situation of tracer liquid, can enumerate following unit: adopt by heavyThe filling rate that quantitative determination and liquid level sensor convert, detects filling situation, sends to and controls dress as signalPut. Specifically, can enumerate Weight measurement device, liquid level sensor or flow sensor etc.
Described control device be to from detecting unit send signal process and by treated signal toThe device that flow controlling unit sends. Be sent to flow-control list by the signal of described control device processingUnit, makes flow controlling unit work as required, controls the flow of liquid. Described control device be preferably byComputer-controlled device.
Flow-control refers to, can be according to the flow control valve that is arranged at the described liquid flow path such as pipe arrangement or nozzleAperture, the filling situation according to liquid in container, controls the flow of liquid. Flow control valve can be establishedPut by storagetank and fill the discharge portion of the pipe arrangement, nozzle interior or the nozzle that are connected with nozzle.
As flow controlling unit, can enumerate the flow-control of flow control valve, valve or pump etc.
In filling device of the present invention, preferred described nozzle is the movable sprinklers that can move up and down. At thisIn situation, preferably filling device further possesses nozzle location control module, its according to liquid in containerFilling situation, the position of the front end of control movable sprinklers.
The so-called movable sprinklers that can move up and down, be can with the rectangular direction of container bottoms onThe nozzle of lower movement.
The signal sending from described detecting unit to control device is as described above by control device processing. InstituteState nozzle and be in the situation of the movable sprinklers that can move up and down, by the signal quilt of described control device processingSend to nozzle location control module, make as required the work of nozzle location control module, before Control NozzleThe position of end. Described control device is preferably by computer-controlled device.
As long as nozzle location control module can be according to liquid the filling situation in container carry out Control NozzleThe position (from bottom surface to the height of spray nozzle front end) of front end can be just any mode.
As nozzle location control module, can enumerate nozzle elevating device or nozzle retractor device etc.
In filling device of the present invention, be preferably configured to storagetank and be positioned at the top of described filling device, utilizeGravity by liquid filling in container.
Storagetank is positioned at the top of this filling device, utilize gravity by liquid filling in container, refer to and stockpileThe liquid level of the liquid of at least one party in the thalline and the handled thing thereof that contain microorganism in storagetank alwaysOn the top of this filling device, i.e. the position higher than the upper cover that is positioned over the container in filling device, do not makeUtilize the aperture of gravity and flow control valve with pump, container is carried and be filled into the liquid in storagetankIn.
The filling device of one aspect of the present invention preferably possesses:
Storagetank, stockpiles the liquid of at least one party in thalline and the handled thing thereof that contains microorganism;
Container, for carrying or preserve described liquid;
Nozzle is discharged the described liquid of carrying from described storagetank in described container;
Detecting unit, detects the filling situation of described liquid in described container; And
Flow controlling unit, the filling situation according to described liquid in described container, controls from described nozzleThe flow of the liquid of discharging.
The filling device of another aspect of the present invention preferably possesses:
Storagetank, stockpiles the liquid of at least one party in thalline and the handled thing thereof that contains microorganism;
Container, for carrying or preserve described liquid;
The movable sprinklers that can move up and down, by the described liquid of carrying from described storagetank to described containerInterior discharge;
Detecting unit, detects the filling situation of described liquid in described container; And
Nozzle location control module, the filling situation according to described liquid in described container, can described in controllingThe position of the front end of moving nozzle.
Fill method of the present invention is the method that uses described filling device, is according to the liquid that makes described nozzleThe mode that discharge portion starts to be arranged in filling liquid midway from the filling of described liquid is carried out described liquid to containerThe method of filling. Specifically, fill method of the present invention has the front-end configuration of nozzle in container,Discharge the filling work procedure of liquid from described front end.
Fill method of the present invention can use aptly possesses described nozzle, detecting unit and flow-controlThe filling device of unit carries out.
In described filling work procedure, preferably make at least one party in the flow at the initial stage of filling and the flow in filling later stageBe less than maximum stream flow.
The filling initial stage represents the stage that is less than 12.5% to the filling rate of container, and the filling later stage represents that filling rate isMore than 97.5% stage. Stage between filling initial stage and filling later stage is called and is filled mid-term, in fillingThe flow of phase is maximum stream flow.
Described flow is from the volume of the liquid time per unit of nozzle discharge, utilizes flowmeter or weightDeterminators etc. are measured.
If at the filling initial stage, almost there is no the flow of the liquid under the state of liquid in container be max-flowAmount, owing to discharging, linear velocity is fast, the contacting or being involved in etc. of air and easy of liquid and container inner wallProduce and bubble. The flow of the liquid by making the initial stage of filling is less than maximum stream flow, can suppress the foaming of liquid,Can improve the charging efficiency of container per unit volume.
In addition, by making the amount of filling the later stage be less than maximum stream flow, thus the adjustment of loading when end-of-fillBecome easily, can improve the charging efficiency of container per unit volume.
The setting value of the filling rate in initial stage, mid-term or later stage is not limited to above-mentioned setting value, preferably basisViscosity, the easy degree of bubbling of the bacterium liquid of the floor space of filling containers and height and filling are carried out optimization.
The maximum stream flow of liquid (filling the flow in mid-term) is preferably with the liquid in the valve of the front end of movable sprinklersThe discharge linear velocity of body reaches the mode of 0.2～3m/ second to be set, more preferably to reach 0.2～2m/ secondMode set.
Described discharge linear velocity is the value that delivery flow is obtained divided by nozzle outlet sectional area, utilizes flowMeter or Weight measurement device etc. are measured.
As long as the flow at filling initial stage is less than maximum stream flow, but from the raising effect excellence of charging efficiencyAspect is considered, is preferably below 1/2 of maximum stream flow. Particularly below 1/5 of maximum stream flow more preferably.
Lower limit is not particularly limited, if but consider to fill required time, be preferably 1/100 of maximum stream flowAbove.
, the range of flow of filling the initial stage is preferably more than 1/100 and below 1/2 of maximum stream flow, more excellentElect as more than 1/100 and below 1/5.
Be less than maximum stream flow as long as fill the flow in later stage, but examine from the aspect of foaming inhibition excellenceConsider, be preferably below 1/2 of maximum stream flow. Particularly below 1/5 of maximum stream flow more preferably. Lower limitBe not particularly limited, if but consider to fill required time, be preferably the more than 1/100 of maximum stream flow.
, the range of flow of filling the later stage is preferably more than 1/100 and below 1/2 of maximum stream flow, more excellentElect as more than 1/100 and below 1/5.
In fill method of the present invention, preferably use and possess the movable sprinklers that can move up and down as sprayMouth, further possesses according to described liquid movable sprinklers described in the filling situation control in described containerThe filling device of the nozzle location control module of front position, at filling initial stage of filling work procedure by removable sprayThe front-end configuration of mouth is near the bottom surface of container, and then the filling situation in container according to liquid, makes to moveMoving nozzle rises.
Near the bottom surface of container, represent 20% the height from bottom surface of container height.
Make movable sprinklers increase according to the filling situation of liquid, refer to from becoming filling liquid and stockpile in containerState time, form be arranged in filling liquid with the liquid discharge portion of movable sprinklers mode by removableNozzle is inserted into the state in liquid. Be present in filling liquid by the liquid discharge portion that makes movable sprinklers,Air can suppress to discharge liquid from movable sprinklers time is involved in. Can suppress thus foaming, the every list of containerThe charging efficiency of position volume improves.
Insert described movable sprinklers in described filling liquid time, from the liquid level of filling liquid to described discharge portionTill length be preferably 1～50cm.
In addition, rise by the liquid discharge portion that makes movable sprinklers according to the rising of liquid level, can be by dippingThe volume of the nozzle in filling liquid is suppressed to Min., thereby improves charging efficiency.
The volume that is immersed in the nozzle in described filling liquid is preferably 0.00002～0.005m3。
As described above, according to filling device of the present invention and fill method, by fill time according to liquidThe filling situation of body in container, at least one party in the position of the front end of control flow and nozzle, therebyFoaming can suppress to fill time, the charging efficiency of raising container per unit volume.
Embodiment
Below, specifically describe the present invention by embodiment and comparative example, but in the scope of its main idea,The present invention is not limited to these.
[embodiment 1]
(1) about embodiment
Fig. 1 is the skeleton diagram that represents the formation of the filling device 10 using in the present embodiment.
Filling device 10 possesses:
Pipe arrangement 1, its upstream side end is connected in and stockpiles in the thalline that contains microorganism and handled thing thereof at leastOne side's liquid L(thallus suspension liquid) storagetank 20;
The movable sprinklers 3 that can move up and down, is located at the downstream terminal part of pipe arrangement 1;
Valve 2, is located at movable sprinklers front end;
Nozzle elevating device 4, moves up and down movable sprinklers 3;
Weight measurement device 5, the weight of mensuration container 30; And
Control device 6, is electrically connected with valve 2, nozzle elevating device 4, Weight measurement device 5.
Above Weight measurement device 5, be placed with container 30, be provided with above it storagetank 20.
Specifically, as valve 2, use following valve: there is lid at spray nozzle front end, by by littlely lid opening,Standard-sized sheet carries out Flow-rate adjustment. Utilize this valve 2 and gravity, the liquid L in storagetank 20 can be filledIn container 30.
As movable sprinklers 3, use the long nozzle type of tracing type.
As nozzle elevating device 4, use oil-pressure lifting device.
As Weight measurement device 5, use load cell formula platform balance.
(2) modulator approach of thallus suspension liquid
Utilization contains glucose 2 quality %, urea 1 quality %, peptone 0.5 quality %, yeast extractionThe culture medium (pH7.0 in the time of 20 DEG C) of thing 0.3 quality %, cobalt chloride 0.05 quality %, to having nitrile waterPrunosus red coccus (Rhodococcusrhodochrous) the J-1 strain (FERMBP-1478) of synthase activityCarry out aerobic cultivation. After cultivation finishes, reclaim and cultivate thalline by centrifugation, used 50mM phosphorusAcid buffer (pH7.0 in the time of 20 DEG C) cleans. In thalline after cleaning, add above-mentioned buffer solution, obtainThallus suspension liquid (dry thalline convert 10 quality %).
(3) filling of thallus suspension liquid from storagetank to container
At 9m3In the storagetank 20 of the stainless steel of capacity, add above-mentioned thallus suspension liquid (dry bacteriumThe body 10 quality % that convert) 3000L, start to fill to container 30. When filling, be 12.5% at filling rateFollowing filling early stage and filling rate are more than 97.5% later stage, make that valve 2 is little to be left, with 0.5L/ minuteFill, filling mid-term, make valve 2 standard-sized sheets, filled with 20L/ minute.
In the time filling, according to the gravimetry value in Weight measurement device 5, utilize control device 6, according toThe mode control that the valve 2 of movable sprinklers 3 front ends is always immersed in thallus suspension liquid with certain depth can moveThe position of moving nozzle 3.
Its result can be filled into thallus suspension liquid in container 30 in suppressing foaming.
Like this, the foaming can suppress to fill thallus suspension liquid time, and can will be immersed in the thalline of fillingThe volume of the movable sprinklers 3 in suspension suppresses for Min., and therefore charging efficiency improves.
Industrial applicibility
According to the present invention, can provide can be by least one party in the thalline that contains microorganism and handled thing thereofEfficient liquid be filled into filling device and the fill method in container. Therefore, the present invention is in industrial workUseful in the utilization of the organism catalyst in skill field etc. etc.
Symbol description
1: pipe arrangement
2: valve
3: movable sprinklers
4: nozzle elevating device
5: Weight measurement device
6: control device
10: filling device
20: storagetank
30: container
L: liquid
Claims (3)
1. a fill method, it is by the thalline that contains microorganism and the processing thereof that stockpile in storagetankThe liquid filling of at least one party in thing is to the fill method in container, the pulverizing that described handled thing is thallineThing, from the extract of thalline or thalline or extract are fixed on to the fixed compound of carrier,
Described fill method has the front-end configuration of the nozzle of filling device in described container, from describedEnd is discharged the filling work procedure of described liquid, and described filling device possesses: nozzle, will carry from described storagetankDescribed liquid in described container, discharge; Detecting unit, detects the filling of described liquid in described containerSituation; And flow controlling unit, the filling situation according to described liquid in described container, controls from instituteState the flow of the liquid of nozzle discharge,
Described nozzle is the movable sprinklers that can move up and down, and makes the liquid discharge portion of described nozzle from describedThe filling of liquid starts to be arranged in filling liquid midway, makes the liquid discharge portion of described nozzle according to the rising of liquid levelRise,
Described filling work procedure comprises to be made the flow at the initial stage of filling and fills the flow in later stage to be less than maximum stream flow, instituteStating the filling initial stage represents the stage that is less than 12.5% to the filling rate of container, and the described filling later stage represents filling rateBe more than 97.5% stage, the stage between described filling initial stage and described filling later stage is called in fillingPhase, the flow in described filling mid-term is maximum stream flow,
Described maximum stream flow with the discharge linear velocity of the liquid in the valve of the front end of described nozzle reach 0.2～The mode of 3m/ second is set,
That the flow at the filling initial stage of described filling work procedure is maximum stream flow is more than 1/100 below 1/2,
That the flow in the filling later stage of described filling work procedure is maximum stream flow is more than 1/100 below 1/2.
2. fill method according to claim 1, it is by the micro-life that contains stockpiling in storagetankThe liquid filling of at least one party in thalline and the handled thing thereof of thing is to the fill method in container,
Described fill method has the front-end configuration of the movable sprinklers of filling device in described container, fromDescribed front end is discharged the filling work procedure of described liquid, and described filling device possesses: what can move up and down movesMoving nozzle is discharged the described liquid of carrying from described storagetank in described container; Detecting unit, detectsThe filling situation of described liquid in described container; And nozzle location control module, exist according to described liquidFilling situation in described container, controls the position of the front end of described movable sprinklers,
At the filling initial stage of described filling work procedure, by the front-end configuration of described movable sprinklers at described containerNear bottom surface, then, the filling situation according to described liquid in described container makes on described movable sprinklersRise, the volume that makes to be immersed in the described movable sprinklers in filling liquid is 0.00002～0.005m3。
3. fill method according to claim 1 and 2, the thalline of described microorganism and handled thing thereofIn at least one party there is nitrile hydratase activity.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011-196036 | 2011-09-08 | ||
| JP2011196036 | 2011-09-08 | ||
| PCT/JP2012/072934 WO2013035851A1 (en) | 2011-09-08 | 2012-09-07 | Filling device and filling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103781897A CN103781897A (en) | 2014-05-07 |
| CN103781897B true CN103781897B (en) | 2016-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280043809.1A Expired - Fee Related CN103781897B (en) | 2011-09-08 | 2012-09-07 | Filling device and fill method |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20140209204A1 (en) |
| JP (1) | JP6052178B2 (en) |
| CN (1) | CN103781897B (en) |
| WO (1) | WO2013035851A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2987622B1 (en) * | 2014-08-20 | 2018-02-21 | Krones AG | Mould filling machine and method of de-moulding and filling containers |
| CN107585337A (en) * | 2017-09-22 | 2018-01-16 | 贵州爽口达食品开发有限公司 | A kind of automatic bottle filling device of apple in syrup can |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011041563A (en) * | 2009-07-24 | 2011-03-03 | Daiyanitorikkusu Kk | Method for preserving microbial cell and suspension of microbial cell |
| WO2011078184A1 (en) * | 2009-12-25 | 2011-06-30 | ダイヤニトリックス株式会社 | Method for producing acrylamide using microbial catalyst |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3918475A (en) * | 1971-10-04 | 1975-11-11 | Pneumatic Scale Corp | Control for filling machine |
| JPH11156177A (en) * | 1997-11-27 | 1999-06-15 | Mitsubishi Paper Mills Ltd | Liquid feeder and its cleaning device |
| WO2001053253A1 (en) * | 2000-01-17 | 2001-07-26 | Mitsui Chemicals, Inc. | Method of purifying amide compound |
| JP5713275B2 (en) * | 2010-06-23 | 2015-05-07 | 三菱レイヨン株式会社 | Container filling method for bacterial cell suspension and / or treated bacterial cell |
| JP5880447B2 (en) * | 2011-01-14 | 2016-03-09 | 三菱レイヨン株式会社 | Method for transporting microbial cells |
-
2012
- 2012-09-07 CN CN201280043809.1A patent/CN103781897B/en not_active Expired - Fee Related
- 2012-09-07 WO PCT/JP2012/072934 patent/WO2013035851A1/en active Application Filing
- 2012-09-07 US US14/343,389 patent/US20140209204A1/en not_active Abandoned
- 2012-09-07 JP JP2013532678A patent/JP6052178B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011041563A (en) * | 2009-07-24 | 2011-03-03 | Daiyanitorikkusu Kk | Method for preserving microbial cell and suspension of microbial cell |
| WO2011078184A1 (en) * | 2009-12-25 | 2011-06-30 | ダイヤニトリックス株式会社 | Method for producing acrylamide using microbial catalyst |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6052178B2 (en) | 2016-12-27 |
| US20140209204A1 (en) | 2014-07-31 |
| WO2013035851A1 (en) | 2013-03-14 |
| CN103781897A (en) | 2014-05-07 |
| JPWO2013035851A1 (en) | 2015-03-23 |
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