CN103781897A - Filling device and filling method - Google Patents
Filling device and filling method Download PDFInfo
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- CN103781897A CN103781897A CN201280043809.1A CN201280043809A CN103781897A CN 103781897 A CN103781897 A CN 103781897A CN 201280043809 A CN201280043809 A CN 201280043809A CN 103781897 A CN103781897 A CN 103781897A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B3/00—Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans, or jars
- B65B3/26—Methods or devices for controlling the quantity of the material fed or filled
- B65B3/30—Methods or devices for controlling the quantity of the material fed or filled by volumetric measurement
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The present invention relates to a filling method that has a filling step in which a valve (2) at the tip of a vertically movable nozzle (3) of a filling device (10) is disposed within a container (30) and a liquid containing at least either or both of cells of a microorganism and a substance treated thereby transferred from a storage tank (20) is sprayed from the valve (2). In the filling step, either or both of an initial flow rate for filling and a later stage flow rate for filling are less than the maximum flow rate. In addition, the present invention relates to a filling method in which, at the initial filling stage in this filling step, the valve (2) at the tip of the movable nozzle (3) is disposed in the proximity of the bottom surface of the container (30), and thereafter, the movable nozzle (3) is made to rise according to the filling conditions for the liquid in the container (30). According to the present invention, a filling device and filling method that can efficiently fill a container with liquid containing at least either or both of cells of a microorganism or a substance treated thereby can be provided.
Description
Technical field
The present invention relates to the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to tamping unit and fill method in container.
The application is based on advocating right of priority No. 2011-196036 in the Japanese Patent Application of Japanese publication on September 8th, 2011, and its content is applied to this.
Background technology
In recent years, have energy-conservation, saving resource the production of useful matter etc., the viewpoint of the biochemical reaction of the feature such as low public hazards technique considers from effectively utilizing, wish utilizes the trend of organism catalyzer prevailing gradually.As organism catalyzer, use the thalline of zooblast, vegetable cell, cell [Dan, the microorganism of the enzyme that contains catalysis goal response, their handled thing etc.As an example of such enzyme, can enumerate the hydratability with itrile group Nitrile hydratase, have itrile group hydrolysis ability nitrilase or there is Ntn hydrolase of the hydrolysis ability of amide group etc.The utilization of these enzymes can realize the low price manufacture of acid amides or carboxylic acid etc. important on chemical industry.
In recent years, the popularization utilizing due to organism catalyzer in industrial technology field, not only for the reactive mode of enzyme reaction, and has proposed improvement or scheme for the peripheral techniques such as store method, fill method of organism catalyzer.For example, in patent documentation 1, for example, as containing the thalline of microorganism that for heat-sterilization microorganism before treatment, contains Nitrile hydratase or the store method of the liquid of bacterial disposing thing, 6～8 methods of ventilating and stirring are proposed the pH of described liquid to be adjusted on one side on one side.
Prior art document
Patent documentation
Patent documentation 1: No. 4205332 communique of Japanese Patent
Summary of the invention
The problem that invention will solve
The thalline that contains microorganism and/or the liquid of its handled thing easily foam.Therefore, there are the following problems: in time foaming from the storage tank of preserving described liquid is filled into filling container, the filling ratio of container per unit volume reduces.
The present invention In view of the foregoing completes, its object be to provide can by the efficient liquid of the thalline that contains microorganism and/or its handled thing be filled into tamping unit and the fill method in container.
Solve the method for problem
The present invention who solves above-mentioned problem has following scheme.
[1] tamping unit, its be by the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to the tamping unit in container,
Described tamping unit possesses nozzle, and this nozzle enters the inside of described container, and the described liquid of carrying from described storagetank is discharged in described container, makes the liquid discharge portion of described nozzle start to be arranged in filling liquid midway from the filling of described liquid.
[2] tamping unit, its be by the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to the tamping unit in container,
Described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge.
[3] tamping unit, its be by the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to the tamping unit in container,
Described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers.
[4] according to the tamping unit described in any one in claim 1～3, be configured to: described storagetank is positioned at the top of this tamping unit, utilize gravity by described liquid filling in described container.
[5], according to the tamping unit described in any one in claim 1～4, the thalline of described microorganism and/or its handled thing have nitrile hydratase activity.
[6] fill method, its be by the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to the fill method in container,
Described fill method has the front-end configuration of the nozzle of tamping unit in described container, discharges the filling work procedure of described liquid from described front end, and described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge,
Described filling work procedure comprises to be made the flow at the initial stage of filling and/or fills the flow in later stage to be less than peak flow.
[7] fill method according to claim 6, the flow at the filling initial stage of described filling work procedure is peak flow below 1/2.
[8], according to the fill method described in claim 6 or 7, the flow in the filling later stage of described filling work procedure is peak flow below 1/2.
[9] fill method, its be by the liquid filling that stockpiles the thalline that contains microorganism in storagetank and/or its handled thing to the fill method in container,
Described fill method has the front-end configuration of the movable sprinklers of tamping unit in described container, discharge the filling work procedure of described liquid from described front end, described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers,
At the filling initial stage of described filling work procedure, by the front-end configuration of described movable sprinklers, near the bottom surface of described container, then, the filling situation according to described liquid in described container rises described movable sprinklers.
[10], according to the fill method described in any one in claim 6～9, the thalline of described microorganism and/or its handled thing have nitrile hydratase activity.
, the present invention relates to following content.
(1) a kind of tamping unit, it is to the tamping unit in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank, described tamping unit possesses nozzle, this nozzle enters the inside of described container, the described liquid of carrying from described storagetank is discharged in described container, make the liquid discharge portion of described nozzle start to be arranged in filling liquid midway from the filling of described liquid;
(2) a kind of tamping unit, it is to the tamping unit in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank, described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge;
(3) a kind of tamping unit, it is to the tamping unit in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank, described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers;
(4) according to the tamping unit described in any one in (1)～(3), be configured to: described storagetank is positioned at the top of this tamping unit, utilize gravity by described liquid filling in described container;
(5) according to the tamping unit described in any one in (1)～(4), at least one party in thalline and the handled thing thereof of described microorganism has nitrile hydratase activity;
(6) a kind of fill method, it is to the fill method in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank, described fill method has the front-end configuration of the nozzle of tamping unit in described container, discharge the filling work procedure of described liquid from described front end, described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge, and described filling work procedure comprises that at least one party in the flow that makes the flow at the initial stage of filling and fill the later stage is less than peak flow;
(7) fill method according to (6), the flow at the filling initial stage of described filling work procedure is peak flow below 1/2;
(8), according to fill method claim (6) or (7) described, the flow in the filling later stage of described filling work procedure is peak flow below 1/2;
(9) a kind of fill method, it is to the fill method in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank, described fill method has the front-end configuration of the movable sprinklers of tamping unit in described container, discharge the filling work procedure of described liquid from described front end, described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, filling situation according to described liquid in described container, control the position of the front end of described movable sprinklers, at the filling initial stage of described filling work procedure, by the front-end configuration of described movable sprinklers near the bottom surface of described container, then, according to described liquid, the filling situation in described container rises described movable sprinklers; And
(10) according to the fill method described in any one in (6)～(9), at least one party in thalline and the handled thing thereof of described microorganism has nitrile hydratase activity.
Invention effect
According to the present invention, can provide can by the efficient liquid of at least one party in the thalline that contains microorganism and handled thing thereof be filled into tamping unit and the fill method in container.
Accompanying drawing explanation
Fig. 1 is the sketch chart that represents the formation of the tamping unit 10 using in embodiment 1.
Embodiment
Tamping unit of the present invention and fill method are to tamping unit and fill method in container by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank.
At this, the thalline of the microorganism using in the present invention can be that viable bacteria body can be also dead thalline.
As the handled thing of thalline, can enumerate the crushed material of thalline (viable bacteria body or dead thalline), from the extract of thalline, thalline or extract are fixed on to the fixed compound of carrier etc.As described extract, preferred enzyme (rough enzyme or refining enzyme).
As the method for fixing thalline or extract, can enumerate entrapping method, crosslinking, or carrier combined techniques etc.So-called entrapping method is the coated method of polymer epithelium that thalline or enzyme is wrapped in the small lattice of high-molecular gel or utilize semi-permeable membranes.So-called crosslinking is by method crosslinked enzyme with the reagent (multi-functional linking agent) with 2 or 2 above functional groups.So-called carrier combined techniques is to make enzyme be incorporated into the method for non aqueous carrier.
As the carrier using in immobilization, can enumerate such as granulated glass sphere, silica dioxide gel, urethane, polyacrylamide, polyvinyl alcohol, carrageenin, Lalgine, agar or gelatin etc.
As at least one party in thalline and the handled thing thereof of microorganism, preferably have and can serve as the enzymic activity that organism catalyzer utilizes.As described enzymic activity, can enumerate such as nitrile hydratase activity, nitrilase activity or lactamase activity etc.In the middle of these, preferably nitrile hydratase activity.
So-called nitrile hydratase activity, refers to that the itrile group that acts on nitrile compounds makes it be converted to the activity of corresponding amide compound.As the measuring method of nitrile hydratase activity, can enumerate the method etc. that is for example determined at the speed of response of the dry thalline of every 1mg while reacting 10 minutes at 10 ℃ of temperature of reaction.
Of the present invention by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the fill method in container, preferably use at least one party in thalline and the handled thing thereof of microorganism of the nitrile hydratase activity with 50～500U/mg.
As at least one party who has in thalline and the handled thing thereof of microorganism of nitrile hydratase activity, the Nitrile hydratase that can enumerate the thalline of the microorganism that contains Nitrile hydratase, extract from described thalline, by described thalline or the immobilized fixed compound of Nitrile hydratase etc.
As the microorganism that contains Nitrile hydratase, just all can use as long as thering is the microorganism that nitrile is converted to the catalyst activity (nitrile hydratase activity) of amides.Can enumerate and for example belong to genus bacillus (Bacillus) genus, sporeless bacterium (Bacterinium) belongs to, micrococci (MicrOcOccus) belongs to, tyrothricin (Brevibacterium) belongs to, coryneform bacteria (Corynebacterium) belongs to, Nocardia (Nocardia) belongs to, pseudomonas (Pseudomonas) belongs to, microbacterium (Microbacterium) belongs to, rhodococcus (Rhodococcus) belongs to, Gordon Salmonella (Gordona) belongs to, vibrios (Vibrio) belongs to, Nitrosomas (Nitrosomonas) belongs to, suis (Streptococcus) belongs to, Bacterium lacticum (Lactobacillus) belongs to, vinelandii (Attotobacter) belong to, yeast (Saccharomyces) belongs to, endomyces (Endomyces) belongs to, aspergillus (Aspergillus) belongs to, mould (Penicillium) belongs to, Mucor (Mucor) belongs to, head mold (Rhizopus) belongs to, achromobacter (Achromobacter) belongs to, Selective medium (Pscudonocardia) belongs to, sickle-like bacteria (Fusarium) belongs to, or the microorganism of edaphic bacillus (Agrobacterium) genus etc., preferably rhodococcus (Rhodococcus) belongs to.
In addition, also can use the form quality conversion body obtaining as follows: obtain the target gene from Nitrile hydratase, nitrilase or the Ntn hydrolase etc. of described microorganism, utilize ordinary method, by described gene directly or improve artificially, described gene is imported to any host (with reference to Molecular Cloning2nd Edition, Cold Spring Habor Laboratory Press, 1989).As such form quality conversion body, can enumerate the intestinal bacteria MT10770(FERM P-14756 that for example uses the Nitrile hydratase of achromobacter (Achromobacter) genus bacterium to carry out form quality conversion) (with reference to Japanese kokai publication hei 8-266277 communique), the intestinal bacteria MT10822(FERM BP-5785 that carries out form quality conversion with the Nitrile hydratase of Selective medium (Pseudonocardia) genus bacterium) (with reference to Japanese kokai publication hei 9-275978 communique), or carry out the microorganism of form quality conversion etc. with the Nitrile hydratase (with reference to Japanese kokai publication hei 4-211379 communique) that prunosus red coccus (Rhodococcus rhodochrous) is planted.
Described microorganism can be used separately any a kind, also two or more can be used in combination.
As the liquid of at least one party in the thalline that contains microorganism and handled thing thereof, can enumerate the nutrient solution that microorganism liquid nutrient medium is cultivated; Separate the resting thalli suspension cleaning as required from nutrient solution; Or the thalline of at low temperatures, resting thalli suspension being pulverized with ultrasonic wave or metal microballon etc. is pulverized liquid etc.
Culture condition (composition, pH or the culture temperature etc. of liquid nutrient medium) can adopt the known culture condition for cultivated microorganism.For example, in the case of the microorganism with nitrile hydratase activity, having added carbon source (carbohydrate such as glucose or fructose), (ammonium sulfate, ammonium chloride or ammonium nitrate etc. are inorganic nitrogen-sourced for nitrogenous source; The organic nitrogen sources such as yeast extract, peptone or meat extract) and the liquid nutrient medium of inorganic salts, metal-salt or VITAMIN etc. as required in, cultivate with 20～40 ℃, pH5～9.Cultivation can suitably adopt shaking culture or stir culture.
The liquid of at least one party in the thalline and the handled thing thereof that contain microorganism obtaining is as described above stored in storagetank conventionally, then, is filled into the container for carrying or preserve described liquid.
Storagetank is preserving described liquid from manufacturing during till in being filled into container, and industrial applicable use is metal, can be the storagetank of the temperature that is suitable for preserving (for example 5 ℃ following) by the temperature control of the liquid stockpiling.
Container is as long as the container that is suitable for filling liquid is just not particularly limited, and can utilize at present the liquid in order to carry or preserve at least one party in thalline and the handled thing thereof that contains microorganism and the container that uses.In general, can use metal system or resinous cylinder or tank etc.As cylinder, conventionally can use the cylinder of internal volume for 200L left and right.As tank, conventionally can use internal volume is 1～10m
3the tank of left and right.
When can filling from this storagetank to described container, tamping unit of the present invention and fill method use.
Tamping unit of the present invention possesses nozzle, and this nozzle enters the inside of container, and the liquid of carrying from storagetank is discharged in container, makes the liquid discharge portion of described nozzle start to be arranged in filling liquid midway from the filling of described liquid.
Described tamping unit preferably possesses according to the liquid discharge portion that makes described nozzle and starts to be arranged in from the filling of described liquid the control device that the mode of filling liquid is controlled midway.
In tamping unit of the present invention, the liquid of carrying from storagetank is entered to internal tank to the nozzle of discharging in container, refer to the inner side that the liquid discharge portion of nozzle is inserted into upper cover from the upper cover of container.
In addition, make the liquid discharge portion of nozzle start to be arranged in filling liquid midway from filling, refer at the filling initial stage owing to there is no filling liquid, therefore be impossible, but while stockpiling the state in container from becoming filling liquid, form the mode that is arranged in liquid with the liquid discharge portion of nozzle nozzle be inserted into the state of liquid., refer to that the liquid discharge portion of nozzle is arranged in the filling liquid of described container.
By carry out the liquid filling to container under such state, the air can be suppressed at from nozzle expel liquid time is involved in.Can suppress thus foaming, the charging efficiency of container per unit volume improves.
The flow controlling unit that tamping unit of the present invention preferably also possesses the filling situation in container detects to liquid detecting unit except described nozzle and the filling situation in container is controlled the flow of the liquid of discharging from nozzle according to liquid.
As the filling situation in container, can enumerate loading level (liquid weight of filling), filling ratio (volume of the liquid of filling is with respect to the ratio of the internal volume of container) or filling stage etc.
As the detecting unit of the filling situation of tracer liquid, can enumerate following unit: adopt the filling ratio converting by weight determination and level sensor, detect filling situation, send to control device as signal.Specifically, can enumerate Weight measurement device, level sensor or flow sensor etc.
Described control device is the device that the signal to sending from detecting unit is processed and treated signal is sent to flow controlling unit.Signal by described control device processing is sent to flow controlling unit, makes as required flow controlling unit work, controls the flow of liquid.Described control device is preferably by computer-controlled device.
Flow control refers to, can be according to the aperture of flowrate control valve that is arranged at the described liquid flow path such as pipe arrangement or nozzle, and the filling situation according to liquid in container, controls the flow of liquid.Flowrate control valve can be arranged on storagetank and the discharge portion of filling the pipe arrangement, nozzle interior or the nozzle that are connected with nozzle.
As flow controlling unit, can enumerate the flow control of flowrate control valve, valve or pump etc.
In tamping unit of the present invention, preferred described nozzle is the movable sprinklers that can move up and down.In this case, preferably tamping unit further possesses nozzle location control unit, and it is the filling situation in container according to liquid, controls the position of the front end of movable sprinklers.
The so-called movable sprinklers that can move up and down is can be at the nozzle moving up and down with the rectangular direction of container bottoms.
The signal sending from described detecting unit to control device is as described above by control device processing.Be the movable sprinklers that can move up and down at described nozzle, be sent to nozzle location control unit by the signal of described control device processing, make as required the work of nozzle location control unit, the position of the front end of Control Nozzle.Described control device is preferably by computer-controlled device.
As long as nozzle location control unit can be according to liquid the position (from bottom surface to the height of spray nozzle front end) of carrying out the front end of Control Nozzle of the filling situation in container can be just any mode.
As nozzle location control unit, can enumerate nozzle elevating device or nozzle retractor device etc.
In tamping unit of the present invention, be preferably configured to storagetank and be positioned at the top of described tamping unit, utilize gravity by liquid filling in container.
Storagetank is positioned at the top of this tamping unit, utilize gravity by liquid filling in container, the liquid level that refers to the liquid that stockpiles at least one party in the thalline and the handled thing thereof that contain microorganism in storagetank always on the top of this tamping unit, i.e. the position higher than the upper cover that is positioned over the container in tamping unit, do not use pump and utilize the aperture of gravity and flow control valve, the liquid in storagetank is carried and is filled in container.
The tamping unit of one aspect of the present invention preferably possesses:
Storagetank, stockpiles the liquid of at least one party in thalline and the handled thing thereof that contains microorganism;
Container, for carrying or preserve described liquid;
Nozzle is discharged the described liquid of carrying from described storagetank in described container;
Detecting unit, detects the filling situation of described liquid in described container; And
Flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge.
The tamping unit of another aspect of the present invention preferably possesses:
Storagetank, stockpiles the liquid of at least one party in thalline and the handled thing thereof that contains microorganism;
Container, for carrying or preserve described liquid;
The movable sprinklers that can move up and down is discharged the described liquid of carrying from described storagetank in described container;
Detecting unit, detects the filling situation of described liquid in described container; And
Nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers.
Fill method of the present invention is to use the method for described tamping unit, is that the mode that starts to be arranged in filling liquid midway from the filling of described liquid according to the liquid discharge portion that makes described nozzle is carried out the method for described liquid to the filling of container.Specifically, fill method of the present invention has the front-end configuration of nozzle in container, from the filling work procedure of described front end expel liquid.
Fill method of the present invention can carry out with the tamping unit that possesses described nozzle, detecting unit and flow controlling unit aptly.
In described filling work procedure, preferably make at least one party in the flow at the initial stage of filling and the flow in filling later stage be less than peak flow.
The filling initial stage represents the stage that is less than 12.5% to the filling ratio of container, and the filling later stage represents that filling ratio is more than 97.5% stage.Stage between filling initial stage and filling later stage is called and is filled mid-term, and the flow of filling mid-term is peak flow.
Described flow is from the volume of the liquid time per unit of nozzle discharge, utilizes under meter or Weight measurement device etc. to measure.
If at the filling initial stage, almost there is no the flow of the liquid under the state of liquid in container be peak flow, owing to discharging, linear velocity is fast, the contacting or being involved in etc. of air and easily produce foaming of liquid and container inner wall.The flow of the liquid by making the initial stage of filling is less than peak flow, can suppress the foaming of liquid, can improve the charging efficiency of container per unit volume.
In addition, by making the amount of filling the later stage be less than peak flow, thereby the adjustment of loading level becomes easily when end-of-fill, can improve the charging efficiency of container per unit volume.
The set(ting)value of the filling ratio in initial stage, mid-term or later stage is not limited to above-mentioned set(ting)value, preferably carries out optimizing according to viscosity, the easy degree of bubbling of the bacterium liquid of the floorage of filling container and height and filling.
The peak flow of liquid (filling the flow in mid-term) preferably reaches the mode of 0.2～3m/ second with the discharge linear velocity of the liquid in the valve of the front end of movable sprinklers to be set, and more preferably sets in the mode that reaches 0.2～2m/ second.
Described discharge linear velocity is the value that delivery flow is obtained divided by nozzle outlet sectional area, utilizes under meter or Weight measurement device etc. to measure.
As long as the flow at filling initial stage is less than peak flow, but from the viewpoint of the raising effect excellence of charging efficiency, be preferably below 1/2 of peak flow.Particularly below 1/5 of peak flow more preferably.
Lower limit is not particularly limited, if but consider to fill required time, be preferably the more than 1/100 of peak flow.
, the flow range of filling the initial stage is preferably more than 1/100 and below 1/2 of peak flow, more preferably more than 1/100 and below 1/5.
Be less than peak flow as long as fill the flow in later stage, but from the viewpoint of foaming inhibition excellence, be preferably below 1/2 of peak flow.Particularly below 1/5 of peak flow more preferably.Lower limit is not particularly limited, if but consider to fill required time, be preferably the more than 1/100 of peak flow.
, the flow range of filling the later stage is preferably more than 1/100 and below 1/2 of peak flow, more preferably more than 1/100 and below 1/5.
In fill method of the present invention, preferably use and possess the movable sprinklers that can move up and down as nozzle, further possess according to described liquid the tamping unit of the nozzle location control unit of the front position of movable sprinklers described in the filling situation control in described container, at filling initial stage of filling work procedure by the front-end configuration of movable sprinklers near the bottom surface at container, then the filling situation in container according to liquid, rises movable sprinklers.
Near the bottom surface of container, represent 20% the height from bottom surface of container height.
Make movable sprinklers increase according to the filling situation of liquid, refer to when becoming filling liquid and stockpile the state in container, form the mode that is arranged in filling liquid with the liquid discharge portion of movable sprinklers movable sprinklers is inserted into the state of liquid.Be present in filling liquid by the liquid discharge portion that makes movable sprinklers, the air can suppress from movable sprinklers expel liquid time is involved in.Can suppress thus foaming, the charging efficiency of container per unit volume improves.
Length insert described movable sprinklers in described filling liquid time, till from the liquid level of filling liquid to described discharge portion is preferably 1～50cm.
In addition, rise by the liquid discharge portion that makes movable sprinklers according to the rising of liquid level, the volume that is immersed in the nozzle in filling liquid can be suppressed to inferior limit, thereby improve charging efficiency.
The volume that is immersed in the nozzle in described filling liquid is preferably 0.00002～0.005m
3.
As described above, according to tamping unit of the present invention and fill method, by the time filling according to liquid the filling situation in container, control at least one party in the position of front end of flow and nozzle, thereby the foaming can suppress to fill time, the charging efficiency of raising container per unit volume.
Embodiment
Below, specifically describe the present invention by embodiment and comparative example, but in the scope of its main idea, the present invention is not limited to these.
[embodiment 1]
(1) about embodiment
Fig. 1 is the sketch chart that represents the formation of the tamping unit 10 using in the present embodiment.
Tamping unit 10 possesses:
Pipe arrangement 1, its upstream side end is connected at least one party's who stockpiles in thalline and the handled thing thereof that contains microorganism liquid L(thallus suspension liquid) storagetank 20;
The movable sprinklers 3 that can move up and down, is located at the downstream side terminal part of pipe arrangement 1;
Nozzle elevating device 4, moves up and down movable sprinklers 3;
Control device 6, is electrically connected with valve 2, nozzle elevating device 4, Weight measurement device 5.
Above Weight measurement device 5, be placed with container 30, be provided with above it storagetank 20.
Specifically, as valve 2, use following valve: there is lid at spray nozzle front end, by by littlely lid opening, standard-sized sheet carries out Flow-rate adjustment.Utilize this valve 2 and gravity, the liquid L in storagetank 20 can be filled in container 30.
As movable sprinklers 3, use the long nozzle type of tracing type.
As nozzle elevating device 4, use oil-pressure lifting device.
As Weight measurement device 5, use load cell formula platform balance.
(2) modulator approach of thallus suspension liquid
The substratum (pH7.0 in the time of 20 ℃) that utilization contains glucose 2 quality %, urea 1 quality %, peptone 0.5 quality %, yeast extract 0.3 quality %, cobalt chloride 0.05 quality %, carries out aerobic cultivation to prunosus red coccus (Rhodococcus rhodochrous) the J-1 strain (FERM BP-1478) with nitrile hydratase activity.After cultivation finishes, reclaim and cultivate thalline by centrifugation, used 50mM phosphoric acid buffer (pH7.0 in the time of 20 ℃) to clean.In thalline after cleaning, add above-mentioned damping fluid, obtain thallus suspension liquid (dry thalline convert 10 quality %).
(3) filling of thallus suspension liquid from storagetank to container
At 9m
3in the storagetank 20 of the stainless steel of capacity, add above-mentioned thallus suspension liquid (dry thalline convert 10 quality %) 3000L, start to fill to container 30.When filling, be that filling below 12.5% early stage and filling ratio are more than 97.5% later stage at filling ratio, make that valve 2 is little to be left, fill with 0.5L/ minute, filling mid-term, make valve 2 standard-sized sheets, filled with 20L/ minute.
In the time filling, according to the weight determination value in Weight measurement device 5, utilize control device 6, be always immersed in the position of the mode control movable sprinklers 3 in thallus suspension liquid with certain depth according to the valve 2 of movable sprinklers 3 front ends.
Its result can be filled into thallus suspension liquid in container 30 in suppressing foaming.
Like this, the foaming can suppress to fill thallus suspension liquid time, and the volume that is immersed in the movable sprinklers 3 in the thallus suspension liquid of filling can be suppressed for inferior limit, therefore charging efficiency improves.
Industrial applicibility
According to the present invention, can provide can by the efficient liquid of at least one party in the thalline that contains microorganism and handled thing thereof be filled into tamping unit and the fill method in container.Therefore, the present invention is useful in utilization of the organism catalyzer in industrial technology field etc. etc.
Nomenclature
1: pipe arrangement
2: valve
3: movable sprinklers
4: nozzle elevating device
5: Weight measurement device
6: control device
10: tamping unit
20: storagetank
30: container
L: liquid
Claims (10)
1. a tamping unit, its be by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the tamping unit in container,
Described tamping unit possesses nozzle, and this nozzle enters the inside of described container, and the described liquid of carrying from described storagetank is discharged in described container, makes the liquid discharge portion of described nozzle start to be arranged in filling liquid midway from the filling of described liquid.
2. a tamping unit, its be by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the tamping unit in container,
Described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge.
3. a tamping unit, its be by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the tamping unit in container,
Described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers.
4. according to the tamping unit described in any one in claim 1～3, be configured to: described storagetank is positioned at the top of this tamping unit, utilize gravity by described liquid filling in described container.
5. according to the tamping unit described in any one in claim 1～4, at least one party in thalline and the handled thing thereof of described microorganism has nitrile hydratase activity.
6. a fill method, its be by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the fill method in container,
Described fill method has the front-end configuration of the nozzle of tamping unit in described container, discharges the filling work procedure of described liquid from described front end, and described tamping unit possesses: nozzle, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And flow controlling unit, the filling situation according to described liquid in described container, controls from the flow of the liquid of described nozzle discharge,
Described filling work procedure comprises that at least one party in the flow that makes the flow at the initial stage of filling and fill the later stage is less than peak flow.
7. fill method according to claim 6, the flow at the filling initial stage of described filling work procedure is peak flow below 1/2.
8. according to the fill method described in claim 6 or 7, the flow in the filling later stage of described filling work procedure is peak flow below 1/2.
9. a fill method, its be by the liquid filling of at least one party in the thalline and the handled thing thereof that contain microorganism that stockpile in storagetank to the fill method in container,
Described fill method has the front-end configuration of the movable sprinklers of tamping unit in described container, discharge the filling work procedure of described liquid from described front end, described tamping unit possesses: the movable sprinklers that can move up and down, the described liquid of carrying from described storagetank is discharged in described container; Detecting unit, detects the filling situation of described liquid in described container; And nozzle location control unit, the filling situation according to described liquid in described container, controls the position of the front end of described movable sprinklers,
At the filling initial stage of described filling work procedure, by the front-end configuration of described movable sprinklers, near the bottom surface of described container, then, the filling situation according to described liquid in described container rises described movable sprinklers.
10. according to the fill method described in any one in claim 6～9, at least one party in thalline and the handled thing thereof of described microorganism has nitrile hydratase activity.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011196036 | 2011-09-08 | ||
| JP2011-196036 | 2011-09-08 | ||
| PCT/JP2012/072934 WO2013035851A1 (en) | 2011-09-08 | 2012-09-07 | Filling device and filling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103781897A true CN103781897A (en) | 2014-05-07 |
| CN103781897B CN103781897B (en) | 2016-05-18 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280043809.1A Expired - Fee Related CN103781897B (en) | 2011-09-08 | 2012-09-07 | Filling device and fill method |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20140209204A1 (en) |
| JP (1) | JP6052178B2 (en) |
| CN (1) | CN103781897B (en) |
| WO (1) | WO2013035851A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2987622B1 (en) * | 2014-08-20 | 2018-02-21 | Krones AG | Mould filling machine and method of de-moulding and filling containers |
| CN107585337A (en) * | 2017-09-22 | 2018-01-16 | 贵州爽口达食品开发有限公司 | A kind of automatic bottle filling device of apple in syrup can |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11156177A (en) * | 1997-11-27 | 1999-06-15 | Mitsubishi Paper Mills Ltd | Liquid feeder and its cleaning device |
| JP2011041563A (en) * | 2009-07-24 | 2011-03-03 | Daiyanitorikkusu Kk | Method for preserving microbial cell and suspension of microbial cell |
| WO2011078184A1 (en) * | 2009-12-25 | 2011-06-30 | ダイヤニトリックス株式会社 | Method for producing acrylamide using microbial catalyst |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3918475A (en) * | 1971-10-04 | 1975-11-11 | Pneumatic Scale Corp | Control for filling machine |
| WO2001053253A1 (en) * | 2000-01-17 | 2001-07-26 | Mitsui Chemicals, Inc. | Method of purifying amide compound |
| JP5713275B2 (en) * | 2010-06-23 | 2015-05-07 | 三菱レイヨン株式会社 | Container filling method for bacterial cell suspension and / or treated bacterial cell |
| WO2012096361A1 (en) * | 2011-01-14 | 2012-07-19 | ダイヤニトリックス株式会社 | Method for transporting microbial cells |
-
2012
- 2012-09-07 CN CN201280043809.1A patent/CN103781897B/en not_active Expired - Fee Related
- 2012-09-07 US US14/343,389 patent/US20140209204A1/en not_active Abandoned
- 2012-09-07 JP JP2013532678A patent/JP6052178B2/en active Active
- 2012-09-07 WO PCT/JP2012/072934 patent/WO2013035851A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11156177A (en) * | 1997-11-27 | 1999-06-15 | Mitsubishi Paper Mills Ltd | Liquid feeder and its cleaning device |
| JP2011041563A (en) * | 2009-07-24 | 2011-03-03 | Daiyanitorikkusu Kk | Method for preserving microbial cell and suspension of microbial cell |
| WO2011078184A1 (en) * | 2009-12-25 | 2011-06-30 | ダイヤニトリックス株式会社 | Method for producing acrylamide using microbial catalyst |
Also Published As
| Publication number | Publication date |
|---|---|
| US20140209204A1 (en) | 2014-07-31 |
| JP6052178B2 (en) | 2016-12-27 |
| WO2013035851A1 (en) | 2013-03-14 |
| CN103781897B (en) | 2016-05-18 |
| JPWO2013035851A1 (en) | 2015-03-23 |
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