CN103773817B - A kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production - Google Patents
A kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production Download PDFInfo
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- CN103773817B CN103773817B CN201410012111.4A CN201410012111A CN103773817B CN 103773817 B CN103773817 B CN 103773817B CN 201410012111 A CN201410012111 A CN 201410012111A CN 103773817 B CN103773817 B CN 103773817B
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- excess sludge
- fermentation
- tea saponin
- anaerobic fermentation
- acid production
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Abstract
The present invention relates to one and utilize tea saponin (TS, Tea? Saponin) method promoting excess sludge anaerobic fermentation and acid production. It is notable not that excess sludge is detested the raising producing acid effect by current chemical surfactant, and chemical surfactant biodegradability is poor, easily cause secondary pollution, and the biosurfactant synthesized by microbial metabolism is relatively costly. The present invention adopts tea saponin to be a Plant Extracts, and the facilitation effect of excess sludge anaerobic fermentation and acid production is notable, produces acid amount height; to environment non-secondary pollution; raw material sources are extensive, with low cost, are suitable for the scale application in excess sludge anaerobic fermentation and acid production process.
Description
Technical field
The present invention relates to the method that one utilizes tea saponin (TS, TeaSaponin) to promote excess sludge anaerobic fermentation and acid production.
Background technology
Current sewage treatment plants many employings activated sludge process carries out sewage disposal, but activated sludge process can produce substantial amounts of excess sludge, and it is higher that it processes cost of disposal, and mishandling meeting brings to environment and has a strong impact on. The anaerobic digestion of mud is the method for the relatively broad sludge stabilizing of current application and resource, and owing to mud containing substantial amounts of Organic substance such as protein and polysaccharide etc., also having researcher to utilize these biomass resources in mud to produce volatile fatty acid (VFAs) by the mode of anaerobic fermentation in recent years, this product can as producing bioabsorbable polymer material (PHA) or the high-quality carbon source of sewage treatment plant's biological decarbonization, dephosphorization.
The anaerobic fermentation process of excess sludge can be divided mainly into four steps, i.e. the dissolving of particulate organic matter, hydrolysis, acidifying and methanation, and wherein the dissolving of particulate organic matter and hydrolysis are the main rate-limiting steps of sludge anaerobic process. In order to improve sludge anaerobic fermentation rate, either physically or chemically excess sludge is carried out pretreatment frequently with some, such as ultrasonic, microwave, heating, enzymolysis, acid adding, add alkali etc. In excess sludge, the existence of chemical surfactant can improve the hydrolysis rate of excess sludge and improve the yield of short-chain fatty acid to have researcher to find in recent years, but the surfactant biodegradability of majority chemosynthesis is poor, environment can be brought adverse influence, and only use chemical surfactant (such as sodium lauryl sulphate or dodecylbenzene sodium sulfonate) that the raising of excess sludge anaerobic acid-production effect is notable not. Additionally, also have the rhamnolipid that researcher utilizes microbial metabolism to synthesize to promote excess sludge anaerobic fermentation and acid production, but fermentable is relatively costly, does not have economy compared with chemical surfactant.
Summary of the invention
Defect that the purpose of the present invention is contemplated to overcome above-mentioned prior art to exist and a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production is provided.
The purpose of the present invention can be achieved through the following technical solutions:
(1) by excess sludge precipitation concentration;
(2) it is added to after tea saponin being dissolved in the excess sludge after step (1) concentrates;
(3) excess sludge is carried out anaerobic fermentation;
(4) fermentation liquid of step (3) is carried out solid-liquid separation and obtain the clear liquid containing volatile fatty acid.
Excess sludge described in step (1) derives from the sewage treatment plant adopting Biochemical method sewage, and the excess sludge sedimentation time is 12～24h, and after precipitation concentration, mud dry weight is 10～20g/L.
The dosage that adds of the tea saponin described in step (2) is 0.02～0.10g (tea saponin)/g (dewatered sludge).
Described in step (3), excess sludge is carried out anaerobic fermentation, start that forward direction anaerobic reactor passes into nitrogen in fermentation to exist to no oxygen in reactor, the fermentation temperature that excess sludge carries out anaerobic fermentation is 28～30 DEG C, it is stirred during fermentation, the speed of mix and blend is 100～120rpm, and fermentation time is 5～12 days.
The mode that solid-liquid separation adopts that fermentation liquid is carried out described in step (4) is centrifugal, and the centrifugal force of centrifugation is 5000～10000g, and centrifugation time is 20min.
The tea saponin that the present invention adopts derives from the extract of seed of Camelliasinensis(L.)Ktzel.
Compared with prior art, the invention have the advantages that
1. the tea saponin adopted is plant extract, has low toxicity, easily biological-degradable and all can keep the feature of excellent surface activity in the scopes such as bigger temperature, pH, to environment non-secondary pollution.
2. the facilitation effect of pair excess sludge anaerobic fermentation and acid production is notable, produces the highest matched group that can less add surfactant of acid amount and improves nearly 4 times.
3. the industrial extraction method of tea saponin is simple, and raw material sources are extensive, with low cost compared with the general biosurfactant produced by biofermentation, is suitable for the scale application in excess sludge anaerobic fermentation and acid production process.
Accompanying drawing explanation
Fig. 1 is in embodiment 1～3 and comparative example, the graph of a relation that in fermentation liquid clear liquid, the concentration of volatile fatty acid changes with fermentation time; WhereinRepresent in comparative example volatile fatty acid with the change of fermentation time,Represent in embodiment 1 volatile fatty acid with the change of fermentation time,Represent in embodiment 2 volatile fatty acid with the change of fermentation time,Represent in embodiment 3 volatile fatty acid with the change of fermentation time;
Fig. 2 is the concentration in embodiment 1～3 and comparative example, when Volatile fatty acid contents reaches the highest in fermentation liquid clear liquid;
Fig. 3 is in embodiment 1～3 and comparative example, the graph of a relation that cumulative methane yield changes with fermentation time; WhereinRepresent in comparative example cumulative methane yield with the change of fermentation time,Represent in embodiment 1 cumulative methane yield with the change of fermentation time,Represent in embodiment 2 cumulative methane yield with the change of fermentation time,Represent in embodiment 3 cumulative methane yield with the change of fermentation time.
Detailed description of the invention
The tea saponin that the present invention adopts derives from the extract of seed of Camelliasinensis(L.)Ktzel.
The present invention is applicable to the anaerobic acid-production promoting to adopt the sewage treatment plant residual mud of bioanalysis. Mud in embodiment adopts Shanghai City secondary sedimentation tank of sewage treatment work excess sludge, this sewage treatment plant inflow is mainly sanitary sewage, adopt A/A/O (Anaerobic-Anoxic-Oxic, anaerobic-anoxic-oxic method) technique, wherein anaerobism section time of staying 1.5h, anoxia section 1.5h, aerobic section 4.7h, mud mud 8～10d in age, sludge loading 0.2kg (BOD5)/(kgMLSS d).In embodiment, MLVSS (VSS concentration, the MixedLiquorVolatileSuspendedSolids)/MLSS (total suspended solid concentration, MixedLiquidSuspendedSolids) of mud is 0.67～0.7.
Embodiment 1
Excess sludge is staticly settled at 4 DEG C the 12～24h concentration to lower floor's thickened sludge between 10～20g/L, remove supernatant, take 300mL thickened sludge to the anaerobic fermentation tank of 1L volume;
Tea saponin dissolves with supernatant obtained in the previous step and is configured to the concentration of 40g/L;
Actual concentrations according to thickened sludge adds tea saponin quick mix homogeneously according to the dosage of 0.02g (tea saponin)/g (dewatered sludge) in fermentation tank;
In fermentation tank, pass into the nitrogen of 10 times of tank volume to remove the oxygen in tank, then begin to excess sludge is carried out anaerobic fermentation, and in anaerobic fermentation process, remain the airtight of fermentation tank;
Maintaining the temperature in fermentation tank in sweat between 28～30 DEG C, the speed of mix and blend is between 100～120rpm, and fermentation reaction carries out 12 days altogether;
In sweat, every day samples 10mL, when 5000～10000g, (selection of centrifugal force is depending on the complexity of solid-liquid separation, the fermentation initial stage is 5000g, along with the carrying out of fermentation gradually rises to 10000g) centrifugal 20min obtains fermentation clear liquid, the VFAs concentration in clear liquid is measured;
In sweat, the methane production in fermentation tank is measured by every day.
Embodiment 2
Excess sludge is staticly settled at 4 DEG C the 12～24h concentration to lower floor's thickened sludge between 10～20g/L, remove supernatant, take 300mL thickened sludge to the anaerobic fermentation tank of 1L volume;
Tea saponin dissolves with supernatant obtained in the previous step and is configured to the concentration of 40g/L;
Actual concentrations according to thickened sludge adds tea saponin quick mix homogeneously according to the dosage of 0.05g (tea saponin)/g (dewatered sludge) in fermentation tank;
In fermentation tank, pass into the nitrogen of 10 times of tank volume to remove the oxygen in tank, then begin to excess sludge is carried out anaerobic fermentation, and in anaerobic fermentation process, remain the airtight of fermentation tank;
Maintaining the temperature in fermentation tank in sweat between 28～30 DEG C, the speed of mix and blend is between 100～120rpm, and fermentation reaction carries out 12 days altogether;
In sweat, every day samples 10mL, when 5000～10000g, (selection of centrifugal force is depending on the complexity of solid-liquid separation, the fermentation initial stage is 5000g, along with the carrying out of fermentation gradually rises to 10000g) centrifugal 20min obtains fermentation clear liquid, the VFAs concentration in clear liquid is measured;
In sweat, the methane production in fermentation tank is measured by every day.
Embodiment 3
Excess sludge is staticly settled at 4 DEG C the 12～24h concentration to lower floor's thickened sludge between 10～20g/L, remove supernatant, take 300mL thickened sludge to the anaerobic fermentation tank of 1L volume;
Tea saponin dissolves with supernatant obtained in the previous step and is configured to the concentration of 40g/L;
Actual concentrations according to thickened sludge adds tea saponin quick mix homogeneously according to the dosage of 0.10g (tea saponin)/g (dewatered sludge) in fermentation tank;
In fermentation tank, pass into the nitrogen of 10 times of tank volume to remove the oxygen in tank, then begin to excess sludge is carried out anaerobic fermentation, and in anaerobic fermentation process, remain the airtight of fermentation tank;
Maintaining the temperature in fermentation tank in sweat between 28～30 DEG C, the speed of mix and blend is between 100～120rpm, and fermentation reaction carries out 12 days altogether;
In sweat, every day samples 10mL, when 5000～10000g, (selection of centrifugal force is depending on the complexity of solid-liquid separation, the fermentation initial stage is 5000g, along with the carrying out of fermentation gradually rises to 10000g) centrifugal 20min obtains fermentation clear liquid, the VFAs concentration in clear liquid is measured;
In sweat, the methane production in fermentation tank is measured by every day.
Comparative example
Excess sludge is staticly settled at 4 DEG C the 12～24h concentration to lower floor's thickened sludge between 10～20g/L, remove supernatant, take 300mL thickened sludge to the anaerobic fermentation tank of 1L volume;
In fermentation tank, pass into the nitrogen of 10 times of tank volume to remove the oxygen in tank, then begin to excess sludge is carried out anaerobic fermentation, and in anaerobic fermentation process, remain the airtight of fermentation tank;
Maintaining the temperature in fermentation tank in sweat between 28～30 DEG C, the speed of mix and blend is between 100～120rpm, and fermentation reaction carries out 12 days altogether;
In sweat, every day samples 10mL, when 5000～10000g, (selection of centrifugal force is depending on the complexity of solid-liquid separation, the fermentation initial stage is 5000g, along with the carrying out of fermentation gradually rises to 10000g) centrifugal 20min obtains fermentation clear liquid, the VFAs concentration in clear liquid is measured;
In sweat, the methane production in fermentation tank is measured by every day.
Day monitoring result such as Fig. 1, the maximum concentration of VFAs such as Fig. 2 in each embodiment to VFAs in embodiment 1～3 and comparative example. Adding tea saponin to significantly improve sludge anaerobic and produce the effect of acid, wherein do not add the highest VFAs concentration of the matched group of tea saponin and occur in the 5th day of fermentation, producing acid amount is 54mg/gDS (DrySludge, dewatered sludge); The each VFAs concentration organizing embodiment the highest having added 0.02～0.10 (g tea saponin)/(gDS) is equally present in the 5th day, and produce acid amount and be respectively increased to 74～207mg/gDS, the highest compared to matched group improve nearly 4 times, and along with adding the raising of dosage, the facilitation effect of sludge anaerobic fermentation and acid production is strengthened gradually.
Day monitoring result such as Fig. 3 to methane in embodiment 1～3 and comparative example. This result shows that adding the tea saponin methane production to excess sludge anaerobic fermentation process has no significant effect, and the methane production of each group embodiment is all close with matched group in whole sweat. Illustrate that add tea saponin does not suppress the generation of methane while remarkably promoting excess sludge anaerobic fermentation and acid production, therefore can realize methane being reclaimed and utilizing simultaneously.
Claims (6)
1. one kind utilizes the method that tea saponin promotes excess sludge anaerobic fermentation and acid production, it is characterised in that the method comprises the following steps:
(1) by excess sludge precipitation concentration;
(2) it is added in the excess sludge of concentration after tea saponin being dissolved;
(3) excess sludge is carried out anaerobic fermentation;
(4) fermentation liquid is carried out solid-liquid separation and obtain the clear liquid of oil-containing volatile fatty acid;
The dosage that adds of the tea saponin described in step (2) is 0.02～0.10g tea saponin/g dewatered sludge.
2. a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production according to claim 1, it is characterised in that the excess sludge described in step (1) derives from the sewage treatment plant adopting Biochemical method sewage.
3. a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production according to claim 1, it is characterised in that the excess sludge sedimentation time described in step (1) is 12～24h, and mud dry weight is 10～20g/L after precipitation concentration.
4. a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production according to claim 1, it is characterized in that, described in step (3), excess sludge is carried out anaerobic fermentation, start that forward direction anaerobic reactor passes into nitrogen in fermentation and exist to no oxygen in reactor.
5. a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production according to claim 1, it is characterized in that, the fermentation temperature that excess sludge carries out anaerobic fermentation described in step (3) is 28～30 DEG C, it is stirred during fermentation, the speed of mix and blend is 100～120rpm, and fermentation time is 5～12 days.
6. a kind of method utilizing tea saponin to promote excess sludge anaerobic fermentation and acid production according to claim 1, it is characterized in that, the mode that solid-liquid separation adopts that fermentation liquid is carried out described in step (4) is centrifugal, the centrifugal force of centrifugation is 5000～10000g, and centrifugation time is 20min.
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| CN105506003A (en) * | 2016-01-05 | 2016-04-20 | 井冈山大学 | Method for promoting ferment acid production of excess sludge through soapnut saponin production leftovers |
| CN105524952B (en) * | 2016-02-04 | 2019-10-01 | 同济大学 | A method of utilizing excess sludge fermentation and acid and synthesized micro-organism grease |
| CN105861572A (en) * | 2016-06-24 | 2016-08-17 | 太原理工大学 | Method for promoting excess sludge carbon source conversion and in-situ synthesis by rhamnolipid biosurfactant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102796764A (en) * | 2012-08-06 | 2012-11-28 | 哈尔滨工业大学 | Method for producing acid through promoting anaerobic fermentation of residual sludge by virtue of rhamnolipid biosurfactant |
| CN103276023A (en) * | 2013-05-27 | 2013-09-04 | 南京理工大学 | Method for promoting anaerobic fermentation of surplus sludge to produce acid by using biosurfactant |
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| CN102796764A (en) * | 2012-08-06 | 2012-11-28 | 哈尔滨工业大学 | Method for producing acid through promoting anaerobic fermentation of residual sludge by virtue of rhamnolipid biosurfactant |
| CN103276023A (en) * | 2013-05-27 | 2013-09-04 | 南京理工大学 | Method for promoting anaerobic fermentation of surplus sludge to produce acid by using biosurfactant |
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| 茶皂素生产工艺及其应用的研究进展;浦炳寅等;《天然产物研究与开发》;19990531;第11卷(第5期);全文 * |
| 茶皂素的开发与应用进展;汪多仁;《江西饲料》;20110228(第2期);第29页左栏倒数第2段至右栏第3段 * |
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