CN103760192A - Dynamic ion flow detection system - Google Patents
Dynamic ion flow detection system Download PDFInfo
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- CN103760192A CN103760192A CN201410013209.1A CN201410013209A CN103760192A CN 103760192 A CN103760192 A CN 103760192A CN 201410013209 A CN201410013209 A CN 201410013209A CN 103760192 A CN103760192 A CN 103760192A
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Abstract
The invention provides a dynamic ion flow detection system. The dynamic ion flow detection system comprises a 3D microscope, a micro electrode, a signal amplifier, a signal modulator, an image collecting/motion controlling/data processing device. The dynamic ion flow detection system provided by the invention can be used for solving the problem of inaccurate micro electrode signal collection caused by plane imaging, realizing the signal 3D detection, and providing a reliable tool for electrophysiology researches.
Description
Technical field
The present invention relates to micrometering technology, relate in particular to a kind of dynamically ion current detection system.
Background technology
Existing non-damage dimension survey technology, under computer control, utilize microelectrode not contact the non-damage mode of sample, to test the local microcell information of sample, as turnover sample ions molecular conecentration, flow rate and flow direction information, sample surfaces local current etc.Measure the microelectrode that different information adopts different qualities: as measured turnover sample ions molecule flowing information, adopt selectivity/specific molecular microelectrode, this microelectrode type has glass microelectrode, metal microelectrode, carbon fiber microelectrodes with micro pipette tips etc., and the ion grace subcategory that can measure comprises H+, Ca
2+, H
+, K
+, Mg
2+, Cl
-, NO
3 -deng, measure sample surfaces local current information and adopt vibration microelectrode etc.
Non-invasive micro-test technology adopts microelectrode to approach the mode collection signal of sample, microelectrode does not contact or invades sample, measuring process to sample without any damaging, injury of lungs is that the distinctive non-damage metering system of survey technology can be applied with biological living to the vast scope sample that flies emperor's body it, biological living sample can be organelle of biological integral, organ, tissue, cellular layer, unicellular and even enrichment etc., and inorganic matter can be metal material, particle object, membrane material etc.
But along with the extensive application of existing non-invasive micro-test technology, its latent defect also comes out.Existing non-invasive micro-test technology is not complete automatic operation, microscope focusing, sample and electrode are placed in the inferior experimenter of needs of microscope the same visual field to carry out by hand, manual operations causes the measuring process cannot standardization, and actual measurement quality is closely related with the level and the experience that realize personnel.A manual operations inevitably otherness also causes the consistance of measurement result poor with repeatability.
Along with the development of photoelectric technology, the system of automatic acquisition object exact image and positional information, tracking object of which movement, as full-automatic image tracing system etc. has formed ripe commercially produced product.The physical image that it obtains and positional information, by software processing, can be converted to the information such as standardization is the most beautiful, resolution, become and implement Based Intelligent Control basis.Control software can send based on standardized information the tracked object of steering order control motion according to default requirement, and adjust steering order according to the real-time measurement result of subject image and positional information, realize Based Intelligent Control.
But ion current detection system of the prior art imaging in the plane, can cause ion current testing result inaccurate, can not realize the large depth of field and detect; Can not realize multi-faceted observation; There is no imaging stereoscopic sensation.Be unfavorable for realizing the relative position of accurate location microelectrode and sample.
Summary of the invention
The invention provides a kind of dynamically ion current detection system, can accurately locate the relative position of microelectrode and sample, thereby realize the accuracy of ion current input.
A kind of dynamic ion current detection system is provided in the present invention, has comprised:
3D microscope, microelectrode, signal amplifier, signal conditioner, image acquisition/motion control/data processor;
Described 3D microscope is used for observing microelectrode, and observed image is sent to image acquisition/motion control/data processor;
In described microelectrode, be filled with liquid ion, for supplying ion to detected vegetable material;
Described signal amplifier amplifies for the signal that microelectrode is collected, and is sent to signal conditioner;
Described signal conditioner carries out A/D conditioning for the signal that signal amplifier is sent, and the signal after conditioning is sent to described image acquisition/motion control/data processor;
Described image acquisition/motion control/data processor is for determining the physiological situation of detected vegetable material according to the signal of the observed image of described 3D microscope transmission and the transmission of described signal conditioner.
Preferably, described microelectrode inside is through silanization processing.
Preferred described image acquisition/device motion control/data processor is also for controlling the microscopical orientation of described 3D.
Ion current data in the signal acquisition microelectrode that preferred described image acquisition/device motion control/data processor sends specifically for the observed image that sends according to described 3D microscope and described signal conditioner, and according to the physiological situation that gets ion current data and determine detected vegetable material.
Dynamic ion current detection system provided by the invention, comprising: 3D microscope, microelectrode, signal amplifier, signal conditioner, image acquisition/motion control/data processor; Described 3D microscope is used for observing microelectrode, and observed image is sent to image acquisition/motion control/data processor; In described microelectrode, be filled with liquid ion, for supplying ion to detected vegetable material; Described signal amplifier amplifies for the signal that microelectrode is collected, and is sent to signal conditioner; Described signal conditioner carries out A/D conditioning for the signal that signal amplifier is sent, and the signal after conditioning is sent to described image acquisition/motion control/data processor; Described image acquisition/motion control/data processor is for determining the physiological situation of detected vegetable material according to the signal of the observed image of described 3D microscope transmission and the transmission of described signal conditioner.Adopt dynamic ion current detection system provided by the invention, can solve the inaccurate problem of microelectrode signals collecting that planar imaging causes, realize the three-dimensional detection of signal, for electrophysiologic studies provides reliable instrument.
Accompanying drawing explanation
The structural representation of the dynamic ion current detection system that Fig. 1 provides for the embodiment of the present invention one;
The structural representation of the dynamic ion current detection system that Fig. 2 provides for the embodiment of the present invention two.
Embodiment
Below in conjunction with drawings and Examples, the specific embodiment of the present invention is further described.Following examples are only for technical scheme of the present invention is more clearly described, and can not limit the scope of the invention with this.
The dynamic ion current detection system that the embodiment of the present invention one provides, as shown in Figure 1, this system comprises:
3D microscope 1, microelectrode 2, signal amplifier 3, signal conditioner 4, image acquisition/motion control/data processor 5;
3D microscope 1 is for observing microelectrode 2, and observed image is sent to image acquisition/motion control/data processor 5;
In microelectrode 2, be filled with liquid ion, for supplying ion to the detected vegetable material 7 of (double dish 6);
Signal amplifier 3 amplifies for the signal that microelectrode 2 is collected, and is sent to signal conditioner 4;
The signal that image acquisition/motion control/data processor 5 sends for the observed image that sends according to 3D microscope 1 and signal conditioner 4 is determined the physiological situation of detected vegetable material 7.
Preferably, microelectrode 2 is the inner microelectrode through silanization processing.
Can make so the most advanced and sophisticated inwall of microelectrode have more adhesion, be conducive to that liquid ion exchanger adheres to and non-leakage to outside.
Preferably, image acquisition/device motion control/data processor 5 is also for controlling the orientation of 3D microscope 1.
In this way, can guarantee the microscopical visual field of 3D on the one hand, make 3D microscope better monitor the relative position of microelectrode and vegetable material, on the other hand, can avoid the microscopical position of manual adjustments, more accurate to microscopical adjusting.
Preferably, ion current data in the signal acquisition microelectrode that image acquisition/device motion control/data processor 5 sends specifically for the observed image that sends according to 3D microscope 1 and signal conditioner 4, and according to the physiological situation that gets ion current data and determine detected vegetable material.
In the embodiment of the present invention, based on Fick law and the Nernst equation ion/molecular characteristics of motion, utilize 3D microscope 1 to observe microelectrode 2 detect vegetable material 7, image is passed on image acquisition device/motion control/data processor 5 and processes and obtain 3-D view; The signal that signal amplifier 3 collects microelectrode 2 is realized preliminary amplification, through signal conditioner, realize A/D signal condition again, ion current data are passed to image acquisition/device motion control/data processor 5 and are processed the most at last, by image acquisition/device motion control/data processor 5, realize three-dimensional image acquisition and to real time 3-D image control and realize ion current data acquisition, by the ion current data that obtain, judge the information such as ion motion speed, direction of motion, thereby judge the physiological situation of vegetable material.
Below in conjunction with concrete application scenarios, to utilizing the process that dynamic ion current detection system that the embodiment of the present invention provides realizes dynamic ion current to describe, using Corn Root Tip Cells as experiment material, measure the absorbing state of its tip of a root to K+: use K
+liquid ion exchanger Potassium ionophore I-cocktail A(sigma company produces), measuring process can be as shown in Figure 2:
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. a dynamic ion current detection system, is characterized in that, comprising:
3D microscope, microelectrode, signal amplifier, signal conditioner, image acquisition/motion control/data processor;
Described 3D microscope is used for observing microelectrode, and observed image is sent to image acquisition/motion control/data processor;
In described microelectrode, be filled with liquid ion, for supplying ion to detected vegetable material;
Described signal amplifier amplifies for the signal that microelectrode is collected, and is sent to signal conditioner;
Described signal conditioner carries out A/D conditioning for the signal that signal amplifier is sent, and the signal after conditioning is sent to described image acquisition/motion control/data processor;
Described image acquisition/motion control/data processor is for determining the physiological situation of detected vegetable material according to the signal of the observed image of described 3D microscope transmission and the transmission of described signal conditioner.
2. the system as claimed in claim 1, is characterized in that, described microelectrode inside is through silanization processing.
3. the system as claimed in claim 1, is characterized in that, described image acquisition/device motion control/data processor is also for controlling the microscopical orientation of described 3D.
4. the system as claimed in claim 1, ion current data in the signal acquisition microelectrode that described image acquisition/device motion control/data processor sends specifically for the observed image that sends according to described 3D microscope and described signal conditioner, and according to the physiological situation that gets ion current data and determine detected vegetable material.
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|---|---|---|---|
| CN201410013209.1A CN103760192A (en) | 2014-01-10 | 2014-01-10 | Dynamic ion flow detection system |
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|---|---|---|---|
| CN201410013209.1A CN103760192A (en) | 2014-01-10 | 2014-01-10 | Dynamic ion flow detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677968A (en) * | 2015-01-22 | 2015-06-03 | 北京农业信息技术研究中心 | Cellular dynamic ion flow detector |
| CN111912993A (en) * | 2019-05-07 | 2020-11-10 | 旭月(北京)科技有限公司 | High-throughput automatic rapid non-damage micrometering system and method |
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| US6038041A (en) * | 1997-09-17 | 2000-03-14 | Virginia Tech Intellectual Properties, Inc. | Three-dimensional holographic fluorescence microscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677968A (en) * | 2015-01-22 | 2015-06-03 | 北京农业信息技术研究中心 | Cellular dynamic ion flow detector |
| CN111912993A (en) * | 2019-05-07 | 2020-11-10 | 旭月(北京)科技有限公司 | High-throughput automatic rapid non-damage micrometering system and method |
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Application publication date: 20140430 |