CN103759995B - Plant chlorophyll measures process simplification device - Google Patents
Plant chlorophyll measures process simplification device Download PDFInfo
- Publication number
- CN103759995B CN103759995B CN201410013817.2A CN201410013817A CN103759995B CN 103759995 B CN103759995 B CN 103759995B CN 201410013817 A CN201410013817 A CN 201410013817A CN 103759995 B CN103759995 B CN 103759995B
- Authority
- CN
- China
- Prior art keywords
- main cylinder
- extracting solution
- buchner funnel
- plant
- mounting bracket
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229930002875 chlorophyll Natural products 0.000 title claims abstract description 26
- 235000019804 chlorophyll Nutrition 0.000 title claims abstract description 26
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 239000007787 solid Substances 0.000 claims abstract description 14
- 238000001802 infusion Methods 0.000 claims abstract description 12
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 241000196324 Embryophyta Species 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 25
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 abstract description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000006004 Quartz sand Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Crushing And Grinding (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of plant chlorophyll and measure process simplification device, including mounting bracket, mounting bracket is provided with transparent reaction cylinder, buchner funnel and volumetric flask；Described buchner funnel is positioned at the bottom of reaction tube, and the liquid outlet of volumetric flask correspondence buchner funnel is arranged；Described reaction tube includes a vertical main cylinder, and the top of main cylinder connects extracting solution infusion appliance and solid matter inlet, and in main cylinder, bottom is provided with liquid valve and woven wire filter；Being additionally provided with crushing cutter in described main cylinder, crushing cutter hangs in mounting bracket；The quantitative filter paper surely plant extraction liquid can being filtered it is provided with in described buchner funnel, grinding, extraction, defecator are combined into an entirety by this device, realize the extraction in the green mensuration process of leaf and filtering processization operation, improve conventional efficient and increase experimental precision, realize batch samples is processed, there is good practical value。
Description
Technical field
The present invention relates to field of experiment equipment, be specifically related to a kind of plant chlorophyll and measure process simplification device。
Background technology
Chlorophyll in plant, measuring chlorophyll content of plant constantly frequently with extracting solution, is extracted by laboratory, then again with the absorbance being in charge of under luminosity measurement wavelength 663mm and 645mm, finally calculates chlorophyllous content。For chlorophyllous extraction, conventional method is constant volume after filtering with mortar grinder, and current lapping device is based on manual operations, and automaticity is not high, it is necessary to spends the substantial amounts of time, and there is also situation about spilling leakage when filtering, causes the waste of experimental resources。
Summary of the invention
In order to solve the problems referred to above, the present invention proposes a kind of plant chlorophyll and measures process simplification device, grinding, extraction, defecator are combined into an entirety by this device, realize the extraction in the green mensuration process of leaf and filtering processization operation, improve conventional efficient and increase experimental precision, realize batch samples is processed, there is good practical value。
The technical solution adopted for the present invention to solve the technical problems is: a kind of plant chlorophyll measures process simplification device, including mounting bracket, mounting bracket is provided with and obtains the transparent reaction cylinder of plant chlorophyll extracting solution, the buchner funnel that plant chlorophyll extracting solution is filtered and collect the volumetric flask of the chlorophyll extracting solution filtered；Described buchner funnel is positioned at the bottom of reaction tube, and the liquid outlet of volumetric flask correspondence buchner funnel is arranged；Described reaction tube includes a vertical main cylinder, and the top of main cylinder connects extracting solution infusion appliance and solid matter inlet, and in main cylinder, bottom is provided with liquid valve and woven wire filter；Being additionally provided with the automatic rotary crushing cutter that can be shredded by plant stem-leaf in described main cylinder, crushing cutter hangs in mounting bracket；The quantitative filter paper that plant extraction liquid is filtered it is provided with in described buchner funnel。
Described mounting bracket includes base, and base is provided with the electronic scale that the liquid in volumetric flask is weighed；The side of base is fixed with vertical supporting bar, support bar connects at least three cross-brace arms, the support arm of top and support bar sliding connection, and its end is provided with the link for hanging crushing cutter, the crushing cutter pressure to reaction tube can be alleviated, middle and bottom support arm end is equipped with adjustment set, it is possible to coincide and is enclosed within reaction tube, the outside of buchner funnel and they is fixed。
The top of described support bar is provided with chute, and one end of the support arm of top is provided with the slide block being connected with slide, and on support bar, the position of corresponding slide block is provided with clamp device, it is possible to fixing support arm。
Described crushing cutter includes being distributed in, by a cutter shaft and circumference, the cutter head that the blade of this cutter shaft bottom forms, and also includes controlling the motor that this cutter head rotates, and the output shaft of described motor is connected with cutter shaft。
Described blade is at least 20。
The angle degree of being not more than of described extracting solution infusion appliance and solid matter inlet and main cylinder。
Described extracting solution infusion appliance takes funnel shaped, and its cervical region is provided with scale, is provided with to control the rotary switch of extracting solution injection speed at top opening and neck junction。
Described solid matter inlet is straight barrel type, is used for adding solid reflection agent。
Described liquid valve includes a baffle plate being connected with main cylinder inwall radial rotating, and the diameter of described baffle plate matches with the internal diameter of main cylinder, and the rotating shaft of baffle plate extends to main cylinder outside and is provided with tightening knob, for controlling the rotation of baffle plate and fixing。
The invention has the beneficial effects as follows: a kind of plant chlorophyll measures process simplification device, grinding, extraction, defecator are combined into an entirety by this device, realize the extraction in the green mensuration process of leaf and filtering processization operation, improve conventional efficient and increase experimental precision, realize batch samples is processed, there is good practical value。
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described。
Accompanying drawing 1 is the structural representation that a kind of plant chlorophyll measures process simplification device。
In figure, 1. mounting bracket, 1-1. base, 1-2. support bar, 1-3. support arm, 2. volumetric flask, 3. buchner funnel, 4. reaction tube, 4-1. main cylinder, 4-2. solid matter inlet, 4-3. extracting solution infusion appliance, 5. crushing cutter, 6. liquid valve, 7. woven wire filter, 8. quantitative filter paper, 9. electronic scale。
Detailed description of the invention
In the accompanying drawings, a kind of plant chlorophyll measures process simplification device, including mounting bracket 1, mounting bracket 1 is provided with and obtains the transparent reaction cylinder 4 of plant chlorophyll extracting solution, the buchner funnel 3 that plant chlorophyll extracting solution is filtered and collect the volumetric flask 2 of the chlorophyll extracting solution filtered；Described buchner funnel 3 is positioned at the bottom of reaction tube 4, and the liquid outlet of the corresponding buchner funnel 3 of volumetric flask 2 is arranged；Described reaction tube 4 includes the top of vertical main cylinder 4-1, a main cylinder 4-1 and connects extracting solution infusion appliance 4-3 and solid matter inlet 4-2, and in main cylinder 4-1, bottom is provided with liquid valve 6 and woven wire filter 7；Being additionally provided with the automatic rotary crushing cutter 5 that can be shredded by plant stem-leaf in described main cylinder 4-1, crushing cutter 5 hangs in mounting bracket 1；The quantitative filter paper 8 that plant extraction liquid is filtered it is provided with in described buchner funnel 3。
Described mounting bracket includes base 1-1, and base 1-1 is provided with the electronic scale 9 that the liquid in volumetric flask 2 is weighed；The side of base 1-1 is fixed with vertical supporting bar 1-2, support bar 1-2 connects at least three cross-brace arm 1-3, the support arm 1-3 of top and support bar 1-2 sliding connection, and its end is provided with the link for hanging crushing cutter 5, the crushing cutter 5 pressure to reaction tube 4 can be alleviated, middle and bottom support arm 1-3 end is equipped with adjustment set, it is possible to coincide and is enclosed within reaction tube 4, the outside of buchner funnel 3 and they is fixed。
The top of described support bar 1-2 is provided with chute, and one end of the support arm 1-3 of top is provided with the slide block being connected with slide, and on support bar 1-2, the position of corresponding slide block is provided with clamp device, it is possible to fixing support arm 1-3。
Described crushing cutter 5 includes being distributed in, by a cutter shaft and circumference, the cutter head that the blade of this cutter shaft bottom forms, and also includes controlling the motor that this cutter head rotates, and the output shaft of described motor is connected with cutter shaft。
Described blade is at least 20。
The angle of described extracting solution infusion appliance 4-3 and solid matter inlet 4-2 and main cylinder 4-1 is not more than 45 degree。
Described extracting solution infusion appliance 4-3 takes funnel shaped, and its cervical region is provided with scale, is provided with to control the rotary switch of extracting solution injection speed at top opening and neck junction。
Described solid matter inlet 4-2 is straight barrel type, is used for adding solid reflection agent。
Described liquid valve 6 includes a baffle plate being connected with main cylinder 4-1 inwall radial rotating, and the diameter of described baffle plate matches with the internal diameter of main cylinder 4-1, and the rotating shaft of baffle plate extends to main cylinder 4-1 outside and is provided with tightening knob, for controlling the rotation of baffle plate and fixing。
Before using, first reaction tube 4, buchner funnel 3 are individually fixed in mounting bracket 1, main cylinder 4-1 and buchner funnel 3 is made to coincide and be connected, the liquid outlet that volumetric flask 2 is directed at buchner funnel 3 is placed, and ensure to placed in buchner funnel 3 quantitative filter paper 8, filter paper acetone-wet, and liquid valve 6 is closed, and opens electronic scale 9 and volumetric flask 2 is carried out empty bottle weighing；During use, the support arm 1-3 of top is moved up and fixes, crushing cutter 5 is made to depart from from main cylinder 4-1, the a certain amount of fresh foliage samples shredded is added in main cylinder 4-1, then again support arm 1-3 is moved down, crushing cutter 5 is made to be placed in main cylinder 4-1, and by solid matter inlet 4-2 to addition quartz sand and Paris white in main cylinder 4-1, 2～3ml80% acetone is added by extracting solution infusion appliance, open motor switch, crushing cutter 5 is made to rotate, leaves is ground into homogenate, add acetone 10ml again, continue to be ground to tissue turned pale, stand 3～5min；Opening liquid valve 6 makes liquid after woven wire filter 7 filters, and enters volumetric flask 2 then through buchner funnel 3 after filtering；Finally it is settled to 25ml with acetone to shake up, namely can be used for chlorophyllous determination experiment。
Claims (6)
1. a plant chlorophyll measures process simplification device, including mounting bracket (1), it is characterized in that: mounting bracket (1) is provided with and obtains the transparent reaction cylinder (4) of plant chlorophyll extracting solution, the buchner funnel (3) that plant chlorophyll extracting solution is filtered and collect the volumetric flask (2) of the chlorophyll extracting solution filtered；Described buchner funnel (3) is positioned at the bottom of reaction tube (4), and the liquid outlet of the corresponding buchner funnel (3) of volumetric flask (2) is arranged；Described reaction tube (4) includes a vertical main cylinder (4-1), the top of main cylinder (4-1) connects extracting solution infusion appliance (4-3) and solid matter inlet (4-2), and main cylinder (4-1) interior bottom is provided with liquid valve (6) and woven wire filter (7)；Being additionally provided with the automatic rotary crushing cutter (5) that can be shredded by plant stem-leaf in described main cylinder (4-1), crushing cutter (5) hangs in mounting bracket (1)；The quantitative filter paper (8) that plant extraction liquid is filtered it is provided with in described buchner funnel (3)；Described mounting bracket (1) includes base (1-1), and base (1-1) is provided with the electronic scale (9) that the liquid in volumetric flask (2) is weighed；The side of base (1-1) is fixed with vertical supporting bar (1-2), support bar (1-2) connects at least three cross-brace arms (1-3), the support arm (1-3) of top and support bar (1-2) sliding connection, and its end is provided with the link for hanging crushing cutter (5), middle and bottom support arm (1-3) end is equipped with adjustment set；The top of described support bar (1-2) is provided with chute, and one end of the support arm (1-3) of top is provided with the slide block being connected with slide, and the position of the upper corresponding slide block of support bar (1-2) is provided with clamp device, it is possible to fixing support arm (1-3)；Described crushing cutter (5) includes being distributed in, by a cutter shaft and circumference, the cutter head that the blade of this cutter shaft bottom forms, and also includes controlling the motor that this cutter head rotates, and the output shaft of described motor is connected with cutter shaft。
2. a kind of plant chlorophyll according to claim 1 measures process simplification device, it is characterized in that: described blade is at least 20。
3. a kind of plant chlorophyll according to claim 1 measures process simplification device, it is characterized in that: the angle of described extracting solution infusion appliance (4-3) and solid matter inlet (4-2) and main cylinder (4-1) is not more than 45 degree。
4. a kind of plant chlorophyll according to claim 1 or 3 measures process simplification device, it is characterized in that: described extracting solution infusion appliance (4-3) takes funnel shaped, its cervical region is provided with scale, is provided with to control the rotary switch of extracting solution injection speed at top opening and neck junction。
5. a kind of plant chlorophyll according to claim 1 or 3 measures process simplification device, it is characterized in that: described solid matter inlet (4-2) is straight barrel type。
6. a kind of plant chlorophyll according to claim 1 measures process simplification device, it is characterized in that: described liquid valve (6) includes a baffle plate being connected with main cylinder (4-1) inwall radial rotating, the diameter of described baffle plate matches with the internal diameter of main cylinder (4-1), and the rotating shaft of baffle plate extends to main cylinder (4-1) outside and is provided with tightening knob。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410013817.2A CN103759995B (en) | 2014-01-13 | 2014-01-13 | Plant chlorophyll measures process simplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410013817.2A CN103759995B (en) | 2014-01-13 | 2014-01-13 | Plant chlorophyll measures process simplification device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103759995A CN103759995A (en) | 2014-04-30 |
| CN103759995B true CN103759995B (en) | 2016-06-22 |
Family
ID=50527270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410013817.2A Expired - Fee Related CN103759995B (en) | 2014-01-13 | 2014-01-13 | Plant chlorophyll measures process simplification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103759995B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITUB20152481A1 (en) * | 2015-07-24 | 2017-01-24 | C I H E A M St Agronomico Mediterraneo | Method for extracting sap from plant material and equipment for implementing the method. |
| CN109115568B (en) * | 2018-07-23 | 2021-03-09 | 上海市农业科学院 | Experimental sample preparation device |
| CN112362440B (en) * | 2020-11-13 | 2021-07-06 | 江西省科学院 | Automatic detection device for chlorophyll a in water body |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19631812A1 (en) * | 1996-08-07 | 1998-02-12 | Basf Lacke & Farben | Sampling unit for working plant e.g. paint, fermentation |
| CN102004049A (en) * | 2010-09-02 | 2011-04-06 | 北京利达科信环境安全技术有限公司 | Preparation method of adjusting standard solution for online detector of chlorophyll A |
| CN103153422A (en) * | 2010-09-15 | 2013-06-12 | 巴斯夫植物科学有限公司 | Extraction device and method for extracting ingredients from a biological sample |
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
-
2014
- 2014-01-13 CN CN201410013817.2A patent/CN103759995B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19631812A1 (en) * | 1996-08-07 | 1998-02-12 | Basf Lacke & Farben | Sampling unit for working plant e.g. paint, fermentation |
| CN102004049A (en) * | 2010-09-02 | 2011-04-06 | 北京利达科信环境安全技术有限公司 | Preparation method of adjusting standard solution for online detector of chlorophyll A |
| CN103153422A (en) * | 2010-09-15 | 2013-06-12 | 巴斯夫植物科学有限公司 | Extraction device and method for extracting ingredients from a biological sample |
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
Non-Patent Citations (1)
| Title |
|---|
| 植物叶片中叶绿素提取方法的比较研究;李志丹等;《广东第二师范学院学报》;20110630;第80-83页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103759995A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103759995B (en) | Plant chlorophyll measures process simplification device | |
| CN105749580B (en) | Two level extraction equipment applied to the extraction of azalea active component | |
| CN206311190U (en) | A kind of Chinese medicine weighing dispensation apparatus | |
| CN203658130U (en) | Device for simplifying process for determining plant chlorophyll | |
| CN102645361A (en) | Automatic treatment device for animal feed AFB1 content detection sample | |
| CN117269091B (en) | System and method for rapidly measuring iodine value of activated carbon | |
| CN208586065U (en) | Water treatment medicine tank with soda acid detection | |
| CN106269092A (en) | Wet type ball mill discharging detecting system | |
| CN109253905B (en) | Automatic sample preparation instrument for pretreatment of solid adsorbent tube | |
| CN114104352B (en) | Tea processing matched tea hair recycling system | |
| CN209342450U (en) | Extraction element is used in a kind of detection of cosmetics | |
| CN108844848B (en) | Air dust detection method in work place | |
| CN202393665U (en) | Full-automatic caking index tester | |
| CN205554665U (en) | Seed partial shipment constant volume gathering device | |
| CN214131512U (en) | Safe treatment device for drugs for anesthesia | |
| CN210825589U (en) | Advanced oxidation sewage treatment equipment | |
| CN211453581U (en) | Agricultural product quality safety inspection device | |
| CN208449176U (en) | A kind of substance qualitative analysis solution preparation device | |
| CN211576689U (en) | Gaseous sample random sampling device | |
| CN213517091U (en) | Novel western medicine moisture detection device | |
| CN211328221U (en) | Economical extraction device for extracting tea polyphenol by adopting ion precipitation method | |
| CN209525169U (en) | A kind of marine monitoring sediment sample pretreatment auxiliary device | |
| CN209342472U (en) | Solid automatic ration beveller | |
| CN109142630B (en) | Device and method for setting feed liquid solid proportion and boiling strength | |
| CN205613550U (en) | Dispensing equipment is selected separately to traditional chinese medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160622 Termination date: 20170113 |