CN103759995A - Plant chlorophyll determination process simplification device - Google Patents
Plant chlorophyll determination process simplification device Download PDFInfo
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- CN103759995A CN103759995A CN201410013817.2A CN201410013817A CN103759995A CN 103759995 A CN103759995 A CN 103759995A CN 201410013817 A CN201410013817 A CN 201410013817A CN 103759995 A CN103759995 A CN 103759995A
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Abstract
The invention relates to a plant chlorophyll determination process simplification device. The plant chlorophyll determination process simplification device comprises a mounting support, and a transparent reaction cylinder, a Buchner funnel and a volumetric flask are arranged on the mounting support, wherein the Buchner funnel is positioned at the lower part of the reaction cylinder, and the volumetric flask is arranged corresponding to a liquid outlet of the Buchner funnel; the reaction cylinder comprises a vertical main cylinder body, the upper part of the main cylinder body is connected with an extract solution injector and a solid substance inlet, and a liquid outlet valve and an iron wire mesh filter are arranged at the lower part in the main cylinder body; a crushing cutter is also arranged in the main cylinder body, and is hung on the mounting support; and quantification filter paper which can filter a plant extract solution is arranged in the Buchner funnel. The device combines grinding, extraction and filtration devices into a whole and can realize the streamlined operation of extraction and filtration in the chlorophyll determination process, improve the experimental efficiency, increase the experimental precision, treat a large batch of samples, and has good practical value.
Description
Technical field
The present invention relates to experimental facilities field, be specifically related to a kind of plant chlorophyll and measure process simplification device.
Background technology
Laboratory often adopts extract when measuring chlorophyll content of plant, the chlorophyll in plant is extracted, and then survey the absorbance under wavelength 663mm and 645mm with point tube photometer, finally calculates chlorophyllous content.For chlorophyllous extraction, conventional method be with mortar grind filter after constant volume, current lapping device is take manual operations as main, automaticity is not high, require a great deal of time, and filter time also there is the situation of spilling leakage, cause the waste of experimental resources.
Summary of the invention
In order to address the above problem, the present invention proposes a kind of plant chlorophyll and measure process simplification device, this device is combined into an entirety by grinding, extraction, filtration unit, realize extraction and filtering processization operation in the green mensuration process of leaf, improve conventional efficient and increase experimental precision, realization is processed batch samples, has good practical value.
The technical solution adopted for the present invention to solve the technical problems is: a kind of plant chlorophyll is measured process simplification device, comprise mounting bracket, mounting bracket is provided with the Buchner funnel that obtains the transparent reaction cylinder of plant chlorophyll extract, plant chlorophyll extract is filtered and the volumetric flask of collecting the chlorophyll extract having filtered; Described Buchner funnel is positioned at the bottom of reaction tube, the liquid outlet setting of the corresponding Buchner funnel of volumetric flask; Described reaction tube comprises a vertical main cylinder, and the top of main cylinder connects extract injector and solid matter entrance, and in main cylinder, bottom is provided with liquid valve and entanglement filtrator; In described main cylinder, be also provided with the automatic rotary crushing cutter that plant stem-leaf can be shredded, crushing cutter hangs on mounting bracket; In described Buchner funnel, be provided with the quantitative filter paper surely plant extraction liquid being filtered.
Described support comprises base, and base is provided with the electronic scales that can weigh to the liquid in volumetric flask; One side of base is fixed with vertical supporting bar, support bar connects at least three cross-brace arms, the sway brace of top and support bar sliding connection, and its end is provided with the link for hanging crushing cutter, can alleviate the pressure of crushing cutter to reaction tube, sway brace end middle and bottom is equipped with regulating sleeve, can coincide and is enclosed within the outside of reaction tube, Buchner funnel and they are fixed.
The top of described support bar is provided with chute, and one end of the sway brace of top is provided with the slide block that can be slidably connected with chute, and on support bar, the position of corresponding slide block is provided with fastener, can fixed support arm.
Described crushing cutter comprises by a cutter shaft and is circumferentially distributed in the cutter head that the blade of this cutter shaft bottom forms, and also comprise and control the motor that this cutter head rotates, the output shaft of described motor is connected with cutter shaft.
Described blade is at least 20.
The angle degree of being not more than of described extract injector and solid matter entrance and main cylinder.
Described extract injector is taked funnel shaped, and its neck is provided with scale, is provided with to control the rotary switch of extract injection speed in top mouth and neck junction.
Described solid matter entrance is straight barrel type, for adding solid reflection agent.
Described liquid valve comprises a baffle plate being connected with main cylinder inwall radial rotating, and the diameter of described baffle plate and the internal diameter of main cylinder match, and the rotating shaft of baffle plate extends to main microbody outside and is provided with tightening knob, for controlling the rotation of baffle plate and fixing.
The invention has the beneficial effects as follows: a kind of plant chlorophyll is measured process simplification device, this device is combined into an entirety by grinding, extraction, filtration unit, realize extraction and filtering processization operation in the green mensuration process of leaf, improve conventional efficient and increase experimental precision, realization is processed batch samples, has good practical value.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
In figure, 1. mounting bracket, 1-1. base, 1-2. support bar, 1-3. sway brace, 2. volumetric flask, 3. Buchner funnel, 4. reaction tube, 4-1. main cylinder, 4-2. solid matter entrance, 4-3. extract injector, 5. crushing cutter, 6. liquid valve, 7. entanglement filtrator, 8. quantitative filter paper, 9. electronic scales.
Embodiment
In the accompanying drawings, a kind of plant chlorophyll is measured process simplification device, comprise mounting bracket 1, mounting bracket 1 is provided with the Buchner funnel 3 that obtains the transparent reaction cylinder 4 of plant chlorophyll extract, plant chlorophyll extract is filtered and the volumetric flask 2 of collecting the chlorophyll extract having filtered; Described Buchner funnel 3 is positioned at the bottom of reaction tube 4, the liquid outlet setting of the corresponding Buchner funnel 3 of volumetric flask 2; Described reaction tube 4 comprises a vertical main cylinder 4-1, and the top of main cylinder 4-1 connects extract injector 4-3 and solid matter entrance 4-2, and in main cylinder 4-1, bottom is provided with liquid valve 6 and entanglement filtrator 7; In described main cylinder 4-1, be also provided with the automatic rotary crushing cutter 5 that plant stem-leaf can be shredded, crushing cutter 5 hangs on mounting bracket 1; In described Buchner funnel 3, be provided with the quantitative filter paper 8 surely plant extraction liquid being filtered.
Described support comprises base 1-1, and base 1-1 is provided with the electronic scales 9 that can weigh to the liquid in volumetric flask 2; A side of base 1-1 is fixed with vertical supporting bar 1-2, support bar 1-2 connects at least three cross-brace arm 1-3, the sway brace 1-3 of top and support bar 1-2 sliding connection, and its end is provided with the link for hanging crushing cutter 5, can alleviate the pressure of crushing cutter 5 to reaction tube 4, sway brace 1-3 end middle and bottom is equipped with regulating sleeve, can coincide and is enclosed within the outside of reaction tube 4, Buchner funnel 3 and they are fixed.
The top of described support bar 1-2 is provided with chute, and one end of the sway brace 1-3 of top is provided with the slide block that can be slidably connected with chute, and the position of the upper corresponding slide block of support bar 1-2 is provided with fastener, can fixed support arm 1-3.
Described crushing cutter 5 comprises by a cutter shaft and is circumferentially distributed in the cutter head that the blade of this cutter shaft bottom forms, and also comprise and control the motor that this cutter head rotates, the output shaft of described motor is connected with cutter shaft.
Described blade is at least 20.
The angle of described extract injector 4-3 and solid matter entrance 4-2 and main cylinder 4-1 is not more than 45 degree.
Described extract injector 4-3 takes funnel shaped, and its neck is provided with scale, is provided with to control the rotary switch of extract injection speed in top mouth and neck junction.
Described solid matter entrance 4-2 is straight barrel type, for adding solid reflection agent.
Described liquid valve 6 comprises a baffle plate being connected with main cylinder 4-1 inwall radial rotating, the internal diameter of the diameter of described baffle plate and main cylinder 4-1 matches, the rotating shaft of baffle plate extends to main microbody 4-1 outside and is provided with tightening knob, for controlling the rotation of baffle plate and fixing.
Before use, first reaction tube 4, Buchner funnel 3 are individually fixed on mounting bracket 1, main cylinder 4-1 and Buchner funnel 3 are coincide to be connected, volumetric flask 2 being aimed to the liquid outlet of Buchner funnel 3 places, and guarantee the interior quantitative filter paper 8 of having placed of Buchner funnel 3, filter paper is moistening with acetone, and liquid valve 6 is in closed condition, opens electronic scales 9 volumetric flask 2 is carried out to empty bottle weighing, during use, the sway brace 1-3 of top is moved up and fixed, crushing cutter 5 is departed from from main cylinder 4-1, in main cylinder 4-1, add a certain amount of fresh leaf sample shredding, and then sway brace 1-3 is moved down, crushing cutter 5 is placed in main cylinder 4-1, and by solid matter entrance 4-2 to addition silica sand and Paris white in main cylinder 4-1, by extract injector, add 2～3ml80% acetone, open motor switch, crushing cutter 5 is rotated, leaf is ground into homogenate, add again acetone 10ml, continuing to be ground to tissue bleaches, standing 3～5min, open liquid valve 6 and make liquid after entanglement filtrator 7 filters, then enter volumetric flask 2 after Buchner funnel 3 filters, finally with acetone, be settled to 25ml and shake up, can be used for chlorophyllous determination experiment.
Claims (9)
1. a plant chlorophyll is measured process simplification device, comprise mounting bracket (1), it is characterized in that: mounting bracket (1) is provided with the volumetric flask (2) that obtains the transparent reaction cylinder (4) of plant chlorophyll extract, the Buchner funnel (3) that plant chlorophyll extract is filtered and collect the chlorophyll extract having filtered; Described Buchner funnel (3) is positioned at the bottom of reaction tube (4), the liquid outlet setting of the corresponding Buchner funnel of volumetric flask (2) (3); Described reaction tube (4) comprises a vertical main cylinder (4-1), the top of main cylinder (4-1) connects extract injector (4-3) and solid matter entrance (4-2), and the interior bottom of main cylinder (4-1) is provided with liquid valve (6) and entanglement filtrator (7); In described main cylinder (4-1), be also provided with the automatic rotary crushing cutter (5) that plant stem-leaf can be shredded, crushing cutter (5) hangs on mounting bracket (1); In described Buchner funnel (3), be provided with the quantitative filter paper (8) surely plant extraction liquid being filtered.
2. a kind of plant chlorophyll according to claim 1 is measured process simplification device, it is characterized in that: described support comprises base (1-1), and base (1-1) is provided with the electronic scales (9) that can weigh to the liquid in volumetric flask (2); One side of base (1-1) is fixed with vertical supporting bar (1-2), support bar (1-2) connects at least three cross-brace arms (1-3), the sway brace (1-3) of top and support bar (1-2) sliding connection, and its end is provided with the link for hanging crushing cutter (5), sway brace (1-3) end middle and bottom is equipped with regulating sleeve.
3. a kind of plant chlorophyll according to claim 1 is measured process simplification device, it is characterized in that: the top of described support bar (1-2) is provided with chute, one end of the sway brace (1-3) of top is provided with the slide block that can be slidably connected with chute, the position of the upper corresponding slide block of support bar (1-2) is provided with fastener, can fixed support arm (1-3).
4. a kind of plant chlorophyll according to claim 1 is measured process simplification device, it is characterized in that: described crushing cutter (5) comprises by a cutter shaft and is circumferentially distributed in the cutter head that the blade of this cutter shaft bottom forms, also comprise and control the motor that this cutter head rotates, the output shaft of described motor is connected with cutter shaft.
5. a kind of plant chlorophyll according to claim 4 is measured process simplification device, it is characterized in that: described blade is at least 20.
6. a kind of plant chlorophyll according to claim 1 is measured process simplification device, it is characterized in that: described extract injector (4-3) and solid matter entrance (4-2) are not more than 45 degree with the angle of main cylinder (4-1).
7. according to a kind of plant chlorophyll described in claim 1 or 6, measure process simplification device, it is characterized in that: described extract injector (4-3) is taked funnel shaped, its neck is provided with scale, is provided with to control the rotary switch of extract injection speed in top mouth and neck junction.
8. according to a kind of plant chlorophyll described in claim 1 or 6, measure process simplification device, it is characterized in that: described solid matter entrance (4-2) is straight barrel type.
9. a kind of plant chlorophyll according to claim 1 is measured process simplification device, it is characterized in that: described liquid valve (6) comprises a baffle plate being connected with main cylinder (4-1) inwall radial rotating, the internal diameter of the diameter of described baffle plate and main cylinder (4-1) matches, and the rotating shaft of baffle plate extends to main microbody (4-1) outside and is provided with tightening knob.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410013817.2A CN103759995B (en) | 2014-01-13 | 2014-01-13 | Plant chlorophyll measures process simplification device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410013817.2A CN103759995B (en) | 2014-01-13 | 2014-01-13 | Plant chlorophyll measures process simplification device |
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| CN103759995A true CN103759995A (en) | 2014-04-30 |
| CN103759995B CN103759995B (en) | 2016-06-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITUB20152481A1 (en) * | 2015-07-24 | 2017-01-24 | C I H E A M St Agronomico Mediterraneo | Method for extracting sap from plant material and equipment for implementing the method. |
| CN109115568A (en) * | 2018-07-23 | 2019-01-01 | 上海市农业科学院 | Laboratory sample preparation facilities |
| CN112362440A (en) * | 2020-11-13 | 2021-02-12 | 江西省科学院 | Automatic detection device for chlorophyll a in water body |
Citations (4)
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| DE19631812A1 (en) * | 1996-08-07 | 1998-02-12 | Basf Lacke & Farben | Sampling unit for working plant e.g. paint, fermentation |
| CN102004049A (en) * | 2010-09-02 | 2011-04-06 | 北京利达科信环境安全技术有限公司 | Preparation method of adjusting standard solution for online detector of chlorophyll A |
| CN103153422A (en) * | 2010-09-15 | 2013-06-12 | 巴斯夫植物科学有限公司 | Extraction device and method for extracting ingredients from a biological sample |
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
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2014
- 2014-01-13 CN CN201410013817.2A patent/CN103759995B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19631812A1 (en) * | 1996-08-07 | 1998-02-12 | Basf Lacke & Farben | Sampling unit for working plant e.g. paint, fermentation |
| CN102004049A (en) * | 2010-09-02 | 2011-04-06 | 北京利达科信环境安全技术有限公司 | Preparation method of adjusting standard solution for online detector of chlorophyll A |
| CN103153422A (en) * | 2010-09-15 | 2013-06-12 | 巴斯夫植物科学有限公司 | Extraction device and method for extracting ingredients from a biological sample |
| CN203658130U (en) * | 2014-01-13 | 2014-06-18 | 中国矿业大学 | Device for simplifying process for determining plant chlorophyll |
Non-Patent Citations (1)
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| 李志丹等: "植物叶片中叶绿素提取方法的比较研究", 《广东第二师范学院学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITUB20152481A1 (en) * | 2015-07-24 | 2017-01-24 | C I H E A M St Agronomico Mediterraneo | Method for extracting sap from plant material and equipment for implementing the method. |
| WO2017017555A1 (en) * | 2015-07-24 | 2017-02-02 | C.I.H.E.A.M Istituto Agronomico Mediterraneo | Method for the extraction of sap from plant material and apparatus for carrying out the method |
| CN109115568A (en) * | 2018-07-23 | 2019-01-01 | 上海市农业科学院 | Laboratory sample preparation facilities |
| CN112362440A (en) * | 2020-11-13 | 2021-02-12 | 江西省科学院 | Automatic detection device for chlorophyll a in water body |
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| CN103759995B (en) | 2016-06-22 |
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