CN103757003A - Litter size related SNP (Single Nucleotide Polymorphism) locus of swine 13# chromosome and primers thereof - Google Patents
Litter size related SNP (Single Nucleotide Polymorphism) locus of swine 13# chromosome and primers thereof Download PDFInfo
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Abstract
The invention relates to SNP (Single Nucleotide Polymorphism) loci related to swine litter size trait, and particularly relates to a litter size related SNP locus of a swine 13# chromosome and primers thereof, belonging to the field of biotechnologies. The locus of the SNP locus is Chr13: 11714625 of a genome version NCBI Build Sscrofa 10. 2; alleles of the locus are T and C and consist of three genotypes, namely C/C, T/C and T/T, and the locus and a flanking sequence thereof are shown in SEQ ID NO: 1. The primers and extension primers for amplifying the locus of the SNP locus are as follows: Chr13-2-PCRU: 5'-ATA ACC CTG TTA CAT TAC CCT ATA TTC C-3'; Chr13-2-PCRL: 5'-AAA AGT AAA ATA TGA CTG TTT GGA AGG-3'; Chr13-2-SNPU: 5'-TGA CTG ACT GAC TGA CTG ACT GAC TGC ATA GTA GAC CAG CTC CCC TAT TT-3'. The litter size of swine with the genotype of T/T is greater than that of the swine with the genotypes of T/C and C/C. The litter size related SNP locus and the primers thereof have the beneficial effects that trait related SNP loci or candidate genes can be screened rapidly in a batch manner by using a whole-genome re-sequencing method, and the locus Chr13: 11714625 can be applied to the genetic breeding of the swine as a genetic marker, so as to increase the litter size of sows.
Description
Technical field:
The present invention relates to the SNP site relevant to pig number born character, be specifically related to SNP site and a primer thereof relevant to litter size on No. 13 karyomit(e)s of pig, belong to biological technical field.
Background technology:
The characters of number born of pig is the chief component of pig reproductive trait, improves litter size of pig significant to the overall economic benefit of raising pig industry.But litter size belongs to the quantitative character that heritability is very low, be difficult to carry out genetic improvement with traditional breeding method.The Taihu Lake pig of China is well-known with high yield, nest litter size is than many 4-5 of nest litter size head of other Chinese native pig breed of great majority and external pig kind (< < Chinese pig breeds will > > Zhang Zhongge chief editor, Science and Technology of Shanghai press, 1986).Erhualian is a kind of of Taihu Lake pig, is the pig kind that reproductivity is the highest in the world.The polymorphic site relevant to high litter size in the pig of Taihu Lake, can be as genetic marker for improving the litter size of sow.
Researchist adopts the method for full genome scanning and candidate gene characters of number born to be carried out to the research of QTL location and candidate gene both at home and abroad.The people such as Li (Li, K., J.Ren, et al.Anim Genet.2009.40 (6): 963-966) utilize and covered 19 chromosomal 183 microsatellite markers pair total litter sizes relevant to litter size of pig of pig, produce the young number of living, produce dead young number and carry out QTL(Quantitative Trait Locus, quantitative trait locus) location.They have studied the F of 299 durocs and the hybridization of Chinese Erhualian
2generation, 6,7, on 8 and No. 15 karyomit(e)s, located and total litter size, produce live young number and the relevant QTL of the dead young number of product.
(the Rothischild such as Rothschild, M., Jacobson, D.et al.Proc Natl Acad Sci U S is (1) A.1996.93: 201-205) by candidate gene method, utilization comprises two extreme mixing breeds of Prunus mume in China mountain system Taihu Lake pig, finds that a gene (ESR) at estrogen receptor seat on No. 1 karyomit(e) and high litter size major gene are chain.RFLPs by female hormone receptor gene seat analyzes, think the homozygous sow (BB type) with 3.7kb band than there is 4.3kb band homozygous sow (AA type) primiparity time want many 2.3 (P<0.01), average voluminous 1.5 (P<0.01) of every tire.At present reported that the gene relevant to litter size also has OPN (US6410227B1), PRLR (Tomas, A., J.Casellas, et al.J Anim Sci.2006.84 (8): 1991-1998), FSH β (Zhao, Y.Li N, et al.Sci China C Life Sci.1998.41 (6): 664-668) and RBP4 (Spotter, A., S.Muller, et al.Reprod Domest Anim.2009.44 (1): 100-105) etc.
Application QTL localization method can be located and the closely linked region of proterties, but need to study a lot of individualities of several generations family, and the cycle is long, wastes time and energy, and cannot complete, and some QTL location is very wide in range in general laboratory.Utilize candidate gene method to study proterties, comparatively speaking simple directly, but what utilize that candidate gene method mainly studies is the sudden change of exon region, and the sudden change of much bringing into play critical function is probably at intron or other functional area; Some complex character is subject to polygene regulation and control; Can not detect all QTL, and some gene with the research of candidate gene method does not find the location of QTL.
Along with development and the maturation of technology, genome and gene annotation are more and more perfect.Due to the fast development of sequencing technologies, the reduction of order-checking cost, can utilize genome sequencing or transcribe group order-checking trait related gene or mutational site are carried out to batch screening.The present invention selects Erhualian (Taihu Lake pig a kind of), hides pig, the fragrant pig of bar horse, and Laiwu Pigs, 5 Chinese native pig breeds such as the southern regions of the Yunnan Province microtia pig and wild boar have carried out the full genome order of resurveying.Wish therefrom to filter out the SNPs(single nucleotide polymorphisms relevant to high litter size, single nucleotide polymorphism) site as genetic marker the genetic breeding for pig, to improve the litter size of sow.
Summary of the invention:
The object of this invention is to provide SNP site and a primer thereof relevant to litter size on No. 13 karyomit(e)s of pig.
The present invention has selected Erhualian (Taihu Lake pig a kind of), hides pig, the fragrant pig of bar horse, and Laiwu Pigs, and the southern regions of the Yunnan Province microtia pig 5 Chinese native pig breeds and wild boar have carried out the full genome order of resurveying.The order number of individuals of resurveying of 6 pig kinds is respectively 11, and 11,9,10,10 and 10.The order sequenced data of resurveying is followed with reference to genome (NCBI Build Sscrofa10.2) and is compared, and utilizes SAMTools software to carry out the extraction in SNPs site.Contriver has compared Erhualian and other 5 pig kinds, finds that a SNP site (Chr13:11714625 of genome version NCBI Build Sscrofa10.2) is relevant to the litter size of pig on No. 13 karyomit(e)s.The allelotrope in this site is T and C, has tri-kinds of genotype of C/C, T/C and T/T.This site and flanking sequence thereof (SEQ ID NO:1) are:
Screen behind this site, contriver be take the DNA of pig as template amplification comprises this site at interior nucleotide sequence fragment and checks order, and this site is verified and correlation analysis.The individuality that wherein contriver selects is the F of white Du Luoke * Erhualian (Taihu Lake pig a kind of)
2offspring.Found that this site and litter size of pig have significant correlation.
Beneficial effect of the present invention is: use the heavy sequence measurement of full genome, can screen fast, in bulk SNP site or the candidate gene relevant to proterties, the litter size of sow can be improved as genetic marker in this site of Chr13:11714625 of genome version NCBI Build Sscrofa10.2 for the genetic breeding of pig.
Accompanying drawing explanation:
Fig. 1 is white Du Luoke * Erhualian F
2the genotypic litter size comparison in 3 kinds, Chr13:11714625 site in godmother pig resource population (when in figure, * represents that two kinds of genotype litter sizes are relatively, 0.01≤P≤0.05;
*represent P < 0.01).
Specific embodiments:
(1) screening in SNP site
The present invention has selected Erhualian (Taihu Lake pig a kind of), hides pig, the fragrant pig of bar horse, and Laiwu Pigs, and the southern regions of the Yunnan Province microtia pig 5 Chinese native pig breeds and wild boar have carried out the full genome order of resurveying.The order number of individuals of resurveying of 6 pig kinds is respectively 11, and 11,9,10,10 and 10.The order sequenced data of resurveying is followed with reference to genome (NCBI Build Sscrofa10.2) and is compared, and utilizes SAMTools software to carry out the extraction in SNPs site.Contriver has compared Erhualian and other 5 pig kinds, finds that a SNP site (Chr13:11714625 of genome version NCBI Build Sscrofa10.2) is relevant to the litter size of pig on No. 13 karyomit(e)s.
(2) evaluation in SNP site
At design of primers website design SNAPSHOT primer, comprise one couple of PCR amplimer (Chr13-2-PCRU:5 '-ATA ACC CTG TTA CAT TAC CCT ATA TTC C-3 '; Chr13-2-PCRL:5 '-AAA AGT AAA ATA TGA CTG TTT GGA AGG-3 ') and extend primer (Chr13-2-SNPU:5 '-TGA CTG ACT GAC TGA CTG ACT GAC TGC ATA GTA GAC CAG CTC CCC TAT TT-3 ').Totally 24 (12 pairs) primers in this site and other 11 sites are respectively got 1 μ l and are mixed into new multiple PCR primer pond.First carry out 12 heavy PCR reactions, reaction system agents useful for same is: multiple PCR primer pond (6.25 μ M each) 8 μ l, dNTP (10mM each) 8 μ l, 10 * PCR Buffer II116 μ l, MgCl
2(25mM Stock) 234 μ l, Fast Start Taq (5U/ μ l) 24 μ l and ddH
2o460 μ l, is mixed into 10 μ l systems by above reagent mixed liquor 8.5 μ l and 1.5 μ l DNA (50ng/ μ l) and carries out 12 heavy pcr amplification reactions.Amplification reaction condition is: 94 ℃ of 4min; 94 ℃ of 30s, 55 ℃ of 30s, 72 ℃ of 1min, totally 40 circulations.Next step carries out PCR reaction purification, and required reagent is: Exo I (10U/ μ l) 60 μ l, SAP (1U/ μ l) 130 μ l, 10 * SAP Buffer35 μ l and ddH
2o125 μ l, reacts by following program after adding the above-mentioned purifying mixed solution of 3.5 μ l centrifugal: 37 ℃ of 40min, 96 ℃ of 10min in the PCR of each sample product.Then carry out single base extension, required reagent is: SNAPSHOT Multiplex ready reaction Mix100 μ l, extension primer pond (6.25 μ M each, 12 are extended primers and respectively get 1 μ l mixing) 8 μ l, 5 * Sequencing Buffer200 μ l and ddH
2o300 μ l, is mixed into 10 μ l systems by the purified product of above reagent mixed liquor 6 μ l and 4 μ l and carries out extension.Extension condition is: 96 ℃ of 10s, 50 ℃ of 5s, 60 ℃ of 30s, totally 25 circulations.Then carry out the purifying of extension product, required reagent is: SAP (1U/ μ l) 120 μ l, 10 * SAP Buffer70 μ l and ddH
2o140 μ l, by following program reacts after adding above-mentioned 3.3 μ l purifying mixed solutions centrifugal in the extension product of each sample: 37 ℃ of 40min, 75 ℃ of 20min, 4 ℃ save backup.High-purity methane amide (Hi-Di Formamide) that each sample is got SNAPSHOT reaction product after the above-mentioned purifying of 5-4 μ l and 5-6 μ l is mixed into 10 μ l systems and is splined on ABI3730XL sequenator after centrifugal and carries out gene type, uses GeneMarker software analysis somatotype result.
(note: the reaction reagent mixed solution consumption of above 4 steps is 100 required amount of reagent of sample, from single base extension, needs lucifuge to carry out.)
(3) white Du Luoke * painted face in Beijing opera reproductive performance hybridization resource population builds
Follow-up confirmatory experiment resource population individual sample used and proterties data thereof are provided by Animal Biotechnology National Key Laboratory of Agricultural University Of Jiangxi cultivation base.
In December, 1998, in Jiang Cong Jiangsu Province, Agricultural University Of Jiangxi's scientific research pig farm, the Erhualian sow of 3 Erhualian conservation field purchases carries out the intersection breeding of blood relationship between field.January calendar year 2001 is according to parent's Farrowing Traits, selected maternal for generations as resource colony of 17 Erhualian sows from the pure breeding offspring of 3 boars and 11 sows.2 high-quality white Duroc boars that Sygen PIC company is so kind as to give are as the male parent for generations of resource family.The F1 generation individuality that these 2 white Du Luoke and the hybridization of 17 Erhualians are produced is bred, and avoids full, and every boar conventionally with same insemination of sows, to obtain the F of large sample
2family half sibs.In January, 2003, March and June, project team is respectively by the 1st crowd and the 2nd crowd of F
2sow is sent to Jiangxi Province's Yichun City herding seed multiplication farm (59), state-run Red Star kind pig farm, Jiangxi Province (52) and quaternionic breeding pig farm, Shangyou County, Jiangxi (118) for the mensuration of sow reproductive trait.
(4) SNP site is at hybridization resource population F
2the gene type of godmother swinery body
According to method above-mentioned steps (2) Suo Shu, with 192 white Du Luoke * Erhualian hybridization resource population F
2the individual DNA of godmother pig is the genotype in this SNP site of template detection.192 F
2for there being 181 successful somatotypes of individuality in resource population individuality, 3 kinds of genotype C/C, the number of individuals of T/C and T/T is respectively 35,88 and 58.
(5) regulating effect of SNP site to characters of number born
At F
2in resource population individuality, with the average litter size of C/C genotype individuality, be 10.605, standard deviation is 0.9531; Average litter size with T/C genotype individuality is 10.713, and standard deviation is 0.8587; Average litter size with T/T genotype individuality is 11.9251, and standard deviation is 0.8829.
The present invention adopts general linear model (GLM) to analyze the impact of SNP site on the total litter size of pig, product young number alive.All statistical study adopts SAS software to carry out.Model is as follows:
Y
ijklm=u+A
i+G
j+B
k+P
l+e
ijklm
Y wherein
ijklmfor the total litter size of pig or product young number alive, u is colony's average, A
ibe i individual additive effect, G
jbe the fixed effect of j SNP loci gene type, B
kfor a batch effect (k=1,2,3,4), P
lfor stochastic effect (l=1,2,3).
Analytical results finds that there is significant correlation (as shown in Figure 1) in this SNP site of Chr13:11714625 with litter size.With the genotypic F of T/T
2the litter size of godmother pig is than with the average many 1.3201(P=0.0325 of the genotypic sow of C/C), with the genotypic F of T/T
2the litter size of godmother pig is than with the average many 1.2121(P=0.017 of the genotypic sow of T/C), as can be seen from Figure 1, minimum with the genotypic number born of sow of C/C.T is for improving the useful allelotrope of litter size of pig.
From SNP site and litter size correlation analysis, find out, this SNP site of Chr13:11714625 can be applied to molecular marker assisted selection (MAS) as molecule marker and improve the litter size of sow.
SEQUENCE LISTING
<110> Kunming Institute of Zoology, Chinese Academy of Sciences
Agricultural University Of Jiangxi
SNP site and the primer thereof relevant to litter size on No. 13 karyomit(e)s of <120> pig
<130> 7
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 601
<212> DNA
<213> Sus scrofa
<400> 1
ggtgtgataa tttctgctat acaacaaagt gattccattt ccatttctct cttggtcagc 60
tgttcagaca atatcccaaa aaaagcttcc acactgttga tcatataaaa gccgcttggt 120
gaacttagga ataagtcggt gccatgactt gtaaaggatt cattccatga cggtcaagtt 180
taagcaggaa tactgtgtca taaccctgtt acattaccct atattcccta ttctggaatc 240
ctctttcacc ctggaactcc cctatgaaaa ccacagcata gtagaccagc tcccctattt 300
tatcattgaa cacccagccc cccatccttc caaacagtca tattttactt ttcagggaag 360
ctacttggta cagtgtcttg aatgttgggc ccctttgtta catgtttgcc acagatcttt 420
gaagaatact caatagggtc ttaatttaaa acattaagat ttaaattatt caaaaactaa 480
ataaatctga caactttcag gtcatagtaa aacattattt atttattttg tctttcttgc 540
cttcaataat ctcacagaga tgacaagtca tgttgcagaa aagaacagta ataatatggg 600
a 601
SEQUENCE LISTING
<110> Kunming Institute of Zoology, Chinese Academy of Sciences
Agricultural University Of Jiangxi
SNP site and the primer thereof relevant to litter size on No. 13 karyomit(e)s of <120> pig
<130> 7
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 28
<212> DNA
<213> Sus scrofa
<400> 1
ataaccctgt tacattaccc tatattcc 28
<210> 2
<211> 27
<212> DNA
<213> Sus scrofa
<400> 2
aaaagtaaaa tatgactgtt tggaagg 27
<210> 3
<211> 50
<212> DNA
<213> Sus scrofa
<400> 3
tgactgactg actgactgac tgactgcata gtagaccagc tcccctattt 50
Claims (8)
1. a SNP site relevant to litter size on No. 13 karyomit(e)s of pig, it is characterized in that: this SNP site is the Chr13:11714625 of genome version NCBI Build Sscrofa10.2, the allelotrope in this site is T and C, has tri-kinds of genotype of C/C, T/C and T/T.
2. the SNP site of claim 1, wherein said three kinds of yielding characteristicses are: with the genotypic litter size of pig of T/T higher than with T/C and the genotypic pig of C/C.
3. the SNP site of claim 1, wherein said allelotrope T is for improving the useful allelotrope of litter size of pig.
4. the SNP site of claim 1, is characterized in that: this SNP site and flanking sequence thereof are as shown in SEQ ID NO:1.
5. the SNP site of claim 1-4, wherein said pig is the offspring of Taihu Lake pig.
6. take the DNA of pig as SNP site described in template amplification comprises claim 1-5 at interior nucleotide sequence fragment.
7. the DNA of pig of take checks order at interior nucleotide sequence fragment to comprising SNP site described in claim 1-5 as template.
8. described in amplification claim 1, the primer in SNP site and extension primer are:
Chr13-2-PCRU：5’-ATA ACC CTG TTA CAT TAC CCT ATA TTC C-3’；
Chr13-2-PCRL：5’-AAA AGT AAA ATA TGA CTG TTT GGA AGG-3’；
Chr13-2-SNPU：5’-TGA CTG ACT GAC TGA CTG ACT GAC TGC ATA
GTA GAC CAG CTC CCC TAT TT-3’。
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| CN105603065A (en) * | 2016-01-04 | 2016-05-25 | 中国科学院昆明动物研究所 | SNP locus related to litter size on sow chromosome 3 and primers thereof |
| CN105821037A (en) * | 2015-01-04 | 2016-08-03 | 深圳华大基因研究院 | Snp marker and application thereof |
| CN111518916A (en) * | 2020-02-28 | 2020-08-11 | 南京农业大学 | SNP (Single nucleotide polymorphism) marker significantly related to pig No. 13 chromosome and Living piglet number of Erhualian pig, and detection method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768642A (en) * | 2010-03-03 | 2010-07-07 | 安徽省农业科学院畜牧兽医研究所 | Molecular marking method for pig litter size |
| CN101818195A (en) * | 2009-11-02 | 2010-09-01 | 华中农业大学 | Genetic marker by taking pig miR-27a precursor flanking sequence SNP as trait of litter size of pig and application |
| CN102080081A (en) * | 2010-11-19 | 2011-06-01 | 华南农业大学 | SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof |
-
2013
- 2013-12-26 CN CN201310727898.8A patent/CN103757003B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101818195A (en) * | 2009-11-02 | 2010-09-01 | 华中农业大学 | Genetic marker by taking pig miR-27a precursor flanking sequence SNP as trait of litter size of pig and application |
| CN101768642A (en) * | 2010-03-03 | 2010-07-07 | 安徽省农业科学院畜牧兽医研究所 | Molecular marking method for pig litter size |
| CN102080081A (en) * | 2010-11-19 | 2011-06-01 | 华南农业大学 | SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| NCBI: "登录号rs326873336", 《DBSNP数据库》, 15 March 2013 (2013-03-15), pages 1 * |
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| CN105483259B (en) * | 2016-01-04 | 2019-05-31 | 中国科学院昆明动物研究所 | SNP site relevant to nest litter size on No. 12 chromosomes of pig |
| CN105603065B (en) * | 2016-01-04 | 2019-05-31 | 中国科学院昆明动物研究所 | SNP site relevant to nest litter size and its primer on No. 3 chromosomes of pig |
| CN105543357B (en) * | 2016-01-04 | 2019-05-31 | 中国科学院昆明动物研究所 | SNP site relevant to nest litter size on No. 4 chromosomes of pig |
| CN111518916A (en) * | 2020-02-28 | 2020-08-11 | 南京农业大学 | SNP (Single nucleotide polymorphism) marker significantly related to pig No. 13 chromosome and Living piglet number of Erhualian pig, and detection method and application thereof |
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