CN103755820A - Cuttlebone polysaccharide for treating empyrosis and preparing process thereof - Google Patents
Cuttlebone polysaccharide for treating empyrosis and preparing process thereof Download PDFInfo
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- CN103755820A CN103755820A CN201310506890.9A CN201310506890A CN103755820A CN 103755820 A CN103755820 A CN 103755820A CN 201310506890 A CN201310506890 A CN 201310506890A CN 103755820 A CN103755820 A CN 103755820A
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- cuttlebone
- polysaccharide
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- water
- centrifugal 30min
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Abstract
The invention discloses a cuttlebone polysaccharide and a preparing process thereof, and is characterized in that the cuttlebone polysaccharide is a polysaccharide prepared from cuttlebone meal. The preparing process comprises the steps: crushing 200 g of the cuttlebone meal, sieving through a 50-mesh sieve, adding 800-1000 ml of water, carrying out 80-90 DEG C water bath for 4 h, centrifuging for 30 min at the speed of 2000-3000 r/min, and taking the supernatant; adjusting the pH value of the supernatant to a protein isoelectric point at the pH of 5.5-6.0 by HCl, centrifuging for 30 min at the speed of 2000-3000 r/min, and discarding the precipitate to remove proteins; concentrating the supernatant to be 1 g/ml, and adding a 95% ethyl alcohol with the volume being 2-5 times of the concentrated supernatant volume for alcohol precipitation; centrifuging for 30 min at the speed of 2000-3000 r/min, collecting the precipitate, redissolving in distilled water, and dialyzing for 24 h with running water; and carrying out freeze drying of the dialysate to obtain the product. The cuttlebone polysaccharide has the advantages that the cuttlebone polysaccharide has the functions of treating empyrosis and promoting wound healing, and is simple in drug administration method and good in compliance.
Description
Technical field: the present invention relates to a kind of scald for the treatment of, promote the polysaccharide preparation field of wound healing.
Technical background:
Endoconcha Sepiae is the inner casing of Sepiidae, and property is salty, puckery, tepor, returns liver, kidney channel, has the effect for the treatment of spontaneous emission and leukorrhagia, astringing to arrest bleeding, relieving gastric hyperacidity to alleviate stomachache, hygroscopic sore.In recent years, many Chinese and overseas scholars use modernism to be studied the pharmacological action of Endoconcha Sepiae and clinical application.Confirm that Endoconcha Sepiae has the effects such as anti-gastric-ulcer, skeletonization, blood coagulation.
Cuttlebone polysaccharide is the polysaccharide extracting from Endoconcha Sepiae.Existing research finds that Cuttlebone polysaccharide has the effect of protection stomach mucous membrane, can be used for treating stomach ulcer and ulcerative enteritis.But at present, have no the effect that Cuttlebone polysaccharide treatment is scalded.
Summary of the invention:
The object of this invention is to provide a kind of Cuttlebone polysaccharide, there is treatment and scald, promote the effect of wound healing.
The polysaccharide that the present invention adopts cuttlefish bone meal to make, preparation technology is: by cuttlefish bone meal 200g, pulverizes, crosses 50 orders, add water 800-1000ml, 80-90 ℃ of water-bath 4h, the centrifugal 30min of 2000-3000r/min, gets supernatant.Use HCl adjust pH to isoelectric points of proteins Ph5.5-6.0 supernatant liquor, the centrifugal 30min of 2000-3000r/min, abandons precipitation except deproteinize.Supernatant concentration, to 1g/ml, is added to 95% ethanol alcohol precipitation of 2-5 times of volume.The centrifugal 30min of 2000-3000r/min, collecting precipitation, redissolves in distilled water, with the flowing water 24h that dialyses.Dialyzate lyophilize, obtains.
Advantage of the present invention is:
Cuttlebone polysaccharide significance reduces scalding area in Rat Scald Model, have the effect that promotes wound healing, and medication is simple, and compliance is good.
Embodiment:
Embodiment 1: by cuttlefish bone meal 200g, pulverizes, crosses 50 orders, add water 1000ml, and 90 ℃ of water-bath 4h, the centrifugal 30min of 3000r/min, gets supernatant.Use HCl adjust pH to isoelectric points of proteins Ph5.5-6.0 supernatant liquor, the centrifugal 30min of 3000r/min, abandons precipitation except deproteinize.Supernatant concentration, to 1g/ml, is added to 95% ethanol alcohol precipitation of 2 times of volumes.The centrifugal 30min of 3000r/min, collecting precipitation, redissolves in distilled water, with the flowing water 24h that dialyses.Dialyzate lyophilize, obtains.
Embodiment 2: by cuttlefish bone meal 200g, pulverizes, crosses 50 orders, add water 800ml, and 90 ℃ of water-bath 4h, the centrifugal 30min of 2000r/min, gets supernatant.Use HCl adjust pH to isoelectric points of proteins Ph5.5-6.0 supernatant liquor, the centrifugal 30min of 2000r/min, abandons precipitation except deproteinize.Supernatant concentration, to 1g/ml, is added to 95% ethanol alcohol precipitation of 5 times of volumes.The centrifugal 30min of 2000r/min, collecting precipitation, redissolves in distilled water, with the flowing water 24h that dialyses.Dialyzate lyophilize, obtains.
Embodiment 3: by cuttlefish bone meal 200g, pulverizes, crosses 50 orders, add water 1000ml, and 80 ℃ of water-bath 4h, the centrifugal 30min of 3000r/min, gets supernatant.Use HCl adjust pH to isoelectric points of proteins Ph5.5-6.0 supernatant liquor, the centrifugal 30min of 3000r/min, abandons precipitation except deproteinize.Supernatant concentration, to 1g/ml, is added to 95% ethanol alcohol precipitation of 4 times of volumes.The centrifugal 30min of 3000r/min, collecting precipitation, redissolves in distilled water, with the flowing water 24h that dialyses.Dialyzate lyophilize, obtains.
Claims (2)
1. Cuttlebone polysaccharide, is characterized in that: the polysaccharide that it is made by cuttlefish bone meal by following raw material.
2. Cuttlebone polysaccharide preparation method, is characterized in that: by cuttlefish bone meal 200g, pulverizing, crosses 50 orders, adds water 800-1000ml, 80-90 ℃ of water-bath 4h, and the centrifugal 30min of 2000-3000r/min, gets supernatant; Use HCl adjust pH to isoelectric points of proteins Ph5.5-6.0 supernatant liquor, the centrifugal 30min of 2000-3000r/min, abandons precipitation except deproteinize; Supernatant concentration, to 1g/ml, is added to 95% ethanol alcohol precipitation of 2-5 times of volume; The centrifugal 30min of 2000-3000r/min, collecting precipitation, redissolves in distilled water, with the flowing water 24h that dialyses; Dialyzate lyophilize, obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310506890.9A CN103755820A (en) | 2013-10-25 | 2013-10-25 | Cuttlebone polysaccharide for treating empyrosis and preparing process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310506890.9A CN103755820A (en) | 2013-10-25 | 2013-10-25 | Cuttlebone polysaccharide for treating empyrosis and preparing process thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103755820A true CN103755820A (en) | 2014-04-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310506890.9A Pending CN103755820A (en) | 2013-10-25 | 2013-10-25 | Cuttlebone polysaccharide for treating empyrosis and preparing process thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN103755820A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837243A (en) * | 2005-11-07 | 2006-09-27 | 中国人民解放军军第二军医大学 | Cuttlebone polysaccharide CPS-1 and its preparation and use |
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2013
- 2013-10-25 CN CN201310506890.9A patent/CN103755820A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837243A (en) * | 2005-11-07 | 2006-09-27 | 中国人民解放军军第二军医大学 | Cuttlebone polysaccharide CPS-1 and its preparation and use |
Non-Patent Citations (4)
| Title |
|---|
| A. SHANMUGAM ET AL.: "Antimicrobial Activity of Polysacchrides Isolated from the Cuttlebone of Sepia aculeata and Sepia brevimana", 《JOURNAL OF FISHERIES AND AQUATIC SCIENCE》, vol. 3, no. 5, 31 May 2008 (2008-05-31) * |
| 唐丽娟等: "不同方法提取海螵蛸多糖及结构表征", 《中成药》, vol. 33, no. 9, 30 September 2011 (2011-09-30) * |
| 张爱华: "《生化分离技术》", 31 October 2012, article "等电点沉淀", pages: 61 * |
| 魏江洲等: "海螵蛸多糖的提取分离及活性组分CPS-1的纯化", 《第二军医大学学报》, vol. 27, 31 January 2006 (2006-01-31) * |
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Application publication date: 20140430 |