CN103749804A - Monascus fagopyrum-tataricum tea and preparation method thereof - Google Patents
Monascus fagopyrum-tataricum tea and preparation method thereof Download PDFInfo
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Abstract
The invention provides monascus fagopyrum-tataricum tea prepared by taking monascus and fagopyrum tataricum as main raw materials. The characteristics of the monascus fagopyrum-tataricum tea comprise that the monascus fagopyrum-tataricum tea is ruddy in color, keeps delicate fragrance of fagopyrum tataricum, is good in mouthfeel, also is relatively high in both lovastatin content and total flavonoid content, and has the substantial effects of reducing blood pressure, reducing blood fat, reducing cholesterol, improving immunity and the like under the synergic effect of monascus and fagopyrum tataricum.
Description
Technical field
The invention belongs to the processing technique field of fermented food, be specifically related to bitter buckwheat tea of a kind of red colouring agent for food, also used as a Chinese medicine and preparation method thereof.
Background technology
Bitter buckwheat is described as " kings of five cereals ", is called " natural gold partner " by international NNFA.On the books in the famous < < Compendium of Materia Medica > > of China: duck wheat nature and flavor hardship, flat, cold, the effect of useful strength, continuous spirit, sharp knowledge, the wide intestines stomach invigorating of sending down abnormally ascending.Traditional medicine and modern medicine all confirm that duck wheat has the effects such as hypoglycemic, reducing blood lipid, urine glucose lowering, anti-constipation, is called " three fall food " and " the 21 century mankind's healthy food " by authoritative expert.In duck wheat, contain abundant nutritional labeling, comprise mineral matter and the trace element of bioflavonoid, multivitamin, 18 seed amino acids and needed by human, wherein the rutin in bioflavonoid has the capillary fragility of reduction, improves microcirculatory effect, and diabetes, hypertension are had to good curative effect.Therefore the general flavone amount in duck wheat can be used as the important indicator of the quality of weighing duck wheat and products thereof.
Tunning-the red colouring agent for food, also used as a Chinese medicine of monascus on rice belongs to natural his spit of fland, its energy comprehensive adjustment blood fat, realize " three fall one liter ", reduce cholesterol, triglyceride, low-density lipoprotein, increasing high density lipoprotein, effectively the effects such as immunity, antifatigue, defaecation ease constipation are slept, are improved in Green Tea Extract, improvement.The Monacolin K(Lovastatin of particularly separating from the metabolite of monascus) be a kind of inhibitors of cholesterol synthesis, and there is the effect of strongly reducing blood fat.
The food variety of producing take duck wheat as raw material is in the market various, has duck wheat oil, the mixed culture fermentation tea-drinking of duck wheat and tealeaves.The patent No. is the preparation method that 201010130837.X discloses tartary buckwheat monascus fermented tea, the preparation method of this invention is: clean duck wheat, pulverize, after adding water boiling, inoculate test tube aspergillus oryzae, by adding, inoculate monascus ruber after molasses sugar and expand and cultivate the liquid seed obtaining and be inoculated in the duck wheat after pulverization process, cultivate after 72 hours for 28-30 ℃, improve deep closet temperature to 50 ℃ and keep 2-5 days, finally add xylitol, splitting.First, the method obtains through aspergillus oryzae fermentation process making liquid seed culture medium used, halfway to its sterilizing time, inoculate monascus ruber and easily cause that different type monascus rubers infects mutually, make the product of generate subsequent output not reach desirable requirement.Secondly, utilizing tartary buckwheat monascus tea that the method obtains because duck wheat is through pulverization process, can make its mouthfeel not good, is only also 1300mg/kg at the last Lovastatin content with effect for reducing fat, general flavone content 2.2%.
Summary of the invention
The invention provides the bitter buckwheat tea of a kind of red colouring agent for food, also used as a Chinese medicine and preparation technology thereof.
The preparation technology of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine of the present invention, is achieved through the following technical solutions, and what it was concrete comprises the steps:
(1) cultivate bacterial classification test tube: be inoculated in the fresh inclined-plane after sterilizing, 28 ℃-30 ℃ cultivation 5-7 days by oese picking slant strains.
(2) preparation of liquid seed liquor: the bacterial classification obtaining in picking step (1) is inoculated in 30 ℃, 200r/min shaking table cultivation 2-3 days in the liquid culture medium after sterilizing, obtains liquid seed liquor.
(3) bitter buckwheat solid fermentation: get duck wheat, the 4-6 that amount of water is duck wheat doubly, soaks and drains after 3-8 hour, put into the steamer of 95-120 ℃, boiling 20 minutes, spreading for cooling, to mixed in the duck wheat after boiling, spread inorganic salt solution, mix after sterilizing the liquid seed liquor that inoculation step (2) obtains, cultivate after 4-5 days for 28-30 ℃, reduce deep closet temperature to 25 ℃, continue to cultivate 8-10 days, discharging, 121 ℃ of oven dry, obtain the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine: get Tartary buckwheat germ, cure 10-30 minute under 80-110 ℃ of condition, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine then obtaining with step (3) mixes according to the ratio of 1:0.5-4, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
In step (1), that described bacterial classification is selected is purple red colouring agent for food, also used as a Chinese medicine MLX2.
In step (1), described sterilizing is sterilizing 20 minutes at 121 ℃.
In step (1), described fresh inclined-plane is brewer's wort solid medium or starch protein culture medium, preferably brewer's wort solid medium, and wherein brewer's wort pol is 8-10Be.
In step (2), described liquid culture medium composition is 3% buckwheat or rice meal or soluble starch according to weight ratio, glucose 3%, peptone 1%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.1%.Wherein, preferred carbon source is buckwheat.
In step (2), described sterilising conditions is sterilizing 20 minutes at 121 ℃.
In step (3), preferably 5 times of described amount of water.
In step (3), described soak time is 3-6 hour, preferably 3-5 hour.
In step (3), described sterilizing is 121 ℃, sterilizing 30 minutes.
In step (3), described inoculum concentration is the 5%-10% of duck wheat weight, preferably 7%-10%.
In step (3), described 28-30 ℃ incubation time preferably 5 days.
In step (3), the incubation time at described 25 ℃, preferably 10 days.
In step (4), described 80-110 ℃, preferably 100 ℃.
In step (4), described 10-30 minute, preferably 20 minutes.
The specific embodiment of form by the following examples, is described in further detail foregoing of the present invention.But this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following embodiment.The technology that all contents of carrying based on the claims in the present invention secretary realize all belongs to scope of the present invention.
The specific embodiment
In experimental example 1 the present invention, in the liquid parameter of planting in subprocess of preparation, select
1, Preparatory work of experiment
Medicine:
Fructus hordei germinatus, rice meal, buckwheat, yeast extract, soluble starch, glucose, peptone, sodium nitrate, potassium dihydrogen phosphate, magnesium sulfate, rare iodine liquid
Instrument and equipment:
Incubator; Drying box; Controllable temperature shaking table; Ultraviolet-uisible spectrophotometer; Electronic balance; Centrifuge; Superclean bench; 500mL and 1000mL triangular flask are some; Test tube is some; Steam sterilization pan
2, experimental technique
(1) preparation of composition brewer's wort in brewer's wort solid medium:
1) fructus hordei germinatus is pulverized, be fine particle shape.In malt flour: the ratio preparation fructus hordei germinatus powder suspension of water (1:4), constant temperature hydrolysis 3 or after 4 hours under 60 ℃, 65 ℃, 70 ℃ conditions respectively, gets a little hydrolyzate in color board, adds after rare iodine liquid, if hydrolyzate presents brown and shows that hydrolysis is complete, carry out next step.
2) boil hydrolyzate 10 minutes, make the enzyme deactivation in brewer's wort.
3) with 3-4 layer gauze, solid residue in Fructus Hordei Germinatus hydrolyzate is filtered out, with saccharometer, measure the pol of filtering stillness of night brewer's wort.
4) brewer's wort is divided and is filled to triangular flask, 121 ℃, sterilizing 20 minutes, saves backup.Filtering precipitate when used next time.
Experimental result is as table 1:
The pol scope of the brewer's wort described in the present invention, at 8-10Be, considers, and finally preferably fructus hordei germinatus constant temperature at 65 ℃ is hydrolyzed 3 hours.
(2) cultivation of bacterial classification test tube:
With brewer's wort solid medium and the freshest inclined-plane of starch protein culture medium, cultivate bacterial classification test tube respectively, observe situation and the holding time of bacterium colony under two kinds of conditions, on request preparation two kinds of culture mediums below:
Brewer's wort solid medium: wherein have 0.5% yeast extract by brewer's wort weight ratio, 2% agar;
Starch protein culture medium: composition is soluble starch 3% by weight, glucose sugar 3%, peptone 1.5%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.1%, agar 2%.
Concrete cultural method is as follows:
Be inoculated in fresh inclined-plane by oese picking slant strains, cultivate 5-7 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
Experimental result is as table 2:
| ? | Brewer's wort solid medium | Starch protein culture medium |
| Bacterial classification | Bacterium colony is large, and product look ability is strong | Bacterium colony is less, a little less than producing look ability |
| Holding time | 5-6 month | 3-4 month |
Result from table 3 shows, bacterial classification is carried out to inclined-plane, and to preserve selected optimal medium be brewer's wort solid medium.
(3) liquid seed culture:
Take weight ratio as 3% buckwheat or rice meal or soluble starch, glucose 3%, peptone 1%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.1% is mixed with three kinds of fluid nutrient mediums containing different carbon sources, get respectively 100ml in the triangular flask of 500ml, with 2 layers of flannelette, on face, adding one deck brown paper covers at bottleneck, with the good bottleneck of line bolt, 121 ℃ of sterilizings are cooled to 30 ℃ after 20 minutes, the test tube strains inoculation obtaining by transfer needle or inoculation shovel picking one fritter above-mentioned steps (2), by postvaccinal triangular flask in 30 ℃, 200r/min shaking table is cultivated 2-3 days, obtain liquid seed.
The situation of observing three kinds of medium culture seed liquor is out as table 3:
By the result in table 3, found out, the liquid seed quality that the medium culture take malt flour as carbon source obtains is good.
Embodiment 2(1) cultivation of test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 5 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 100ml in the triangular flask of 500ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking one fritter step (1), postvaccinal triangular flask is cultivated 2 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 20kg of Liangshan, Sichuan, adds the water of 4 times of duck wheat weight, soaks and drains after 3 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 33 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 5%.At 28-30 ℃, constant temperature culture is after 4 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 8 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 80 ℃ of conditions, cure 30 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine obtaining with step (3) mixes according to the ratio of 1:4, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat tea of the red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3480mg/kg; Adopt colorimetric determination, general flavone content 3591mg/kg.
Embodiment 3
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 6 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 200ml in the triangular flask of 1000ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking two fritter steps (1), postvaccinal triangular flask is cultivated 3 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 40kg of Liangshan, Sichuan, adds the water of 5 times of duck wheat weight, soaks and drains after 4 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 34 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 7%.At 28-30 ℃, constant temperature culture is after 5 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 9 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 90 ℃ of conditions, cure 20 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine then obtaining with step (3) mixes according to the ratio of 1:0.5, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3631mg/kg; Adopt colorimetric determination, general flavone content 3756mg/kg.
Embodiment 4
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 7 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 200ml in the triangular flask of 1000ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking one fritter step (1), postvaccinal triangular flask is cultivated 2 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 60kg of Liangshan, Sichuan, adds the water of 6 times of duck wheat weight, soaks and drains after 5 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 35 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 10%.At 28-30 ℃, constant temperature culture is after 5 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 10 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 100 ℃ of conditions, cure 30 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine then obtaining with step (3) mixes according to the ratio of 1:2, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3886mg/kg; Adopt colorimetric determination, general flavone content 4007mg/kg.
Embodiment 5
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 6 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 100ml in the triangular flask of 500ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking one fritter step (1), postvaccinal triangular flask is cultivated 2 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 30kg of Liangshan, Sichuan, adds the water of 5 times of duck wheat weight, soaks and drains after 6 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 35 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 9%.At 28-30 ℃, constant temperature culture is after 4 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 9 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 110 ℃ of conditions, cure 10 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine that Tartary buckwheat germ obtains with step (3) mixes according to the ratio of 1:3, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat tea of the red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3642mg/kg; Adopt colorimetric determination, general flavone content 3912mg/kg.
Embodiment 6
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 7 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 200ml in the triangular flask of 1000ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking two fritter steps (1), postvaccinal triangular flask is cultivated 3 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 50kg of Liangshan, Sichuan, adds the water of 4 times of duck wheat weight, soaks and drains after 8 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 33 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 5%.At 28-30 ℃, constant temperature culture is after 5 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 10 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 90 ℃ of conditions, cure 20 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine then obtaining with step (3) mixes according to the ratio of 1:1, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3499mg/kg; Adopt colorimetric determination, general flavone content 3655mg/kg.
Embodiment 7
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 5 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 100ml in the triangular flask of 500ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking one fritter step (1), postvaccinal triangular flask is cultivated 3 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 20kg of Liangshan, Sichuan, adds the water of 5 times of duck wheat weight, soaks and drains after 4 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 35 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 10%.At 28-30 ℃, constant temperature culture is after 5 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 10 days, dries at 121 ℃, obtains the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine.
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine:
Get Tartary buckwheat germ, under 100 ℃ of conditions, cure 20 minutes, the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine then obtaining with step (3) mixes according to the ratio of 1:1, and packing obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 4098mg/kg; Adopt colorimetric determination, general flavone content 4211mg/kg.
Reference embodiment 1
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 5 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 200ml in the triangular flask of 1000ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking two fritter steps (1), postvaccinal triangular flask is cultivated 3 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 20kg of Liangshan, Sichuan, adds the water of 5 times of duck wheat weight, soaks and drains after 4 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 35 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 10%.At 28-30 ℃, constant temperature culture is after 5 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 10 days, dries at 121 ℃, and packing, obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat tea of the red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3975mg/kg; Adopt colorimetric determination, general flavone content 3189mg/kg.
Reference embodiment 2
(1) cultivation of the test tube strains of purple red colouring agent for food, also used as a Chinese medicine MLX2:
By oese picking slant strains, be inoculated in fresh inclined-plane (slant medium is brewer's wort solid medium) upper, cultivate 7 days, obtain cultured bacterial classification test tube for 28 ℃-30 ℃.
(2) preparation of liquid seed:
The culture medium prescription that is carbon source by buckwheat is mixed with liquid culture medium, get 200ml in the triangular flask of 1000ml, at 121 ℃, sterilizing is cooled to 30 ℃ after 20 minutes, with the bacterial classification test tube obtaining in transfer needle or inoculation shovel picking two fritter steps (1), postvaccinal triangular flask is cultivated 2 days in 30 ℃, 200r/min shaking table, obtained liquid seed.
(3) bitter buckwheat solid fermentation:
Taking the place of production is the duck wheat 30kg of Liangshan, Sichuan, adds the water of 4 times of duck wheat weight, soaks and drains after 6 hours, put into temperature at 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, the mixed sodium nitrate that spreads the duck wheat dry weight 0.2% after dissolving by suitable quantity of water in the duck wheat after boiling, 0.15% potassium dihydrogen phosphate, 0.1% magnesium sulfate, after being mixed, sterilizing 30 minutes under 121 ℃ of conditions, is cooled to after 35 ℃, the liquid seed obtaining in inoculation step (2), inoculum concentration is 8%.At 28-30 ℃, constant temperature culture is after 4 days, deep closet temperature dropped to 25 ℃ and continue to cultivate dischargings after 9 days, dries at 121 ℃, and packing, obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
According to the bitter buckwheat tea of the red colouring agent for food, also used as a Chinese medicine making under above-mentioned condition, adopt high performance liquid chromatography to detect, wherein the content of Lovastatin is 3656mg/kg; Adopt colorimetric determination, general flavone content 2980mg/kg.
In sum, with embodiment 1-embodiment 6, the Lovastatin in the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine that the condition of reference embodiment 1-reference embodiment 2 is made and general flavone content are as table 4:
| ? | Lovastatin content (mg/kg) | General flavone content (mg/kg) |
| Embodiment 2 | 3480 | 3591 |
| Embodiment 3 | 3631 | 3756 |
| Embodiment 4 | 3886 | 4007 |
| Embodiment 5 | 3642 | 3912 |
| Embodiment 6 | 3499 | 3655 |
| Embodiment 7 | 4098 | 4211 |
| Reference embodiment 1 | 3975 | 3189 |
| Reference embodiment 2 | 3656 | 2980 |
In table 4, result can be found out, the general flavone content in the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine making with the condition of reference embodiment is obviously low than the general flavone content in the bitter buckwheat of the red colouring agent for food, also used as a Chinese medicine making with the condition in embodiment.So the bitter buckwheat tea of the red colouring agent for food, also used as a Chinese medicine of producing with the preparation technology in the present invention has not only retained the delicate fragrance of duck wheat, mouthfeel is good, and Lovastatin and the general flavone content in tea is high simultaneously, can effectively play three and fall, and improves the effects such as immunity.
Claims (10)
1. a preparation method for the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine, is characterized in that comprising the following steps:
(1) cultivate bacterial classification test tube: be inoculated in fresh inclined-plane, 28 ℃-30 ℃ cultivation 5-7 days by oese picking slant strains;
(2) preparation of liquid seed liquor: the bacterial classification obtaining in picking step (1) is inoculated in 30 ℃, 200r/min shaking table cultivation 2-3 days in the liquid culture medium after sterilizing, obtains liquid seed liquor;
(3) bitter buckwheat solid fermentation: get duck wheat, the 4-6 that amount of water is duck wheat doubly, soaks after 3-8 hour, drain, put into 95-120 ℃ of steam steamer, boiling 20 minutes, spreading for cooling, spreads inorganic salt solution to mixed in the duck wheat after boiling, mixes after sterilizing, the liquid seed liquor that inoculation step (2) obtains, cultivates after 4-5 days for 28-30 ℃, reduces deep closet temperature to 25 ℃, continue to cultivate 9-10 days, discharging, 121 ℃ of oven dry, obtain the bitter buckwheat of red colouring agent for food, also used as a Chinese medicine;
(4) preparation of the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine: get Tartary buckwheat germ, after high temperature cures, mix according to the ratio of 1:0.5-4 with the bitter buckwheat of the red colouring agent for food, also used as a Chinese medicine obtaining in step (3), packing, obtains the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine.
2. preparation method according to claim 1, that the bacterial classification described in it is characterized in that is selected is purple red colouring agent for food, also used as a Chinese medicine MXL2.
3. preparation method according to claim 1, is characterized in that it is brewer's wort solid medium or starch protein culture medium that the middle test tube strains of described step (1) is cultivated culture medium used.
4. according to the preparation method described in claim 1,3, it is characterized in that malt extract medium composition used in step (1) also contains 0.5% yeast extract (by weight percentage) except brewer's wort, 2% agar (by weight percentage); Starch protein culture medium composition is soluble starch 3% by weight, glucose sugar 3%, peptone 1.5%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.1%, agar 2%.
5. according to claim 3,4, described preparation method, it is characterized in that the preparation method of brewer's wort is for to pulverize fructus hordei germinatus, in the ratio of malt flour: water=1:4, make fructus hordei germinatus powder suspension, at 60-70 ℃ of constant temperature hydrolysis 3-4 hour, hydrolyzate is boiled 10 minutes, filter, obtain brewer's wort, preferably the pol of brewer's wort is 8-10Be.
6. preparation method according to claim 1, the constituent that it is characterized in that the liquid culture medium described in step (2) is 3% buckwheat or rice meal or soluble starch by weight, glucose 3%, peptone 1%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.1%.
7. preparation method according to claim 1, it is characterized in that the duck wheat weight described in step (3) is 20-100 part, described inorganic salt solution is 0.2% the sodium nitrate by dry duck wheat weight, 0.15% potassium dihydrogen phosphate, and 0.1% magnesium sulfate is dissolved in water and obtains.
8. preparation method according to claim 1, it is characterized in that described in step (3) inoculum concentration be the 5%-10% of duck wheat weight, preferred inoculum concentration is 7%-10%.
9. preparation method according to claim 1, is characterized in that the stoving temperature described in step (4) is 80-110 ℃, and the time of curing is 10-30 minute, wherein preferably 100 ℃, 20 minutes.
10. the bitter buckwheat tea of red colouring agent for food, also used as a Chinese medicine making according to the preparation method described in claim 1-10.
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| CN105166232A (en) * | 2015-09-21 | 2015-12-23 | 神农架华峰生物科技有限公司 | Manufacturing method of red kojic rice tea |
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