CN103743869A - Determination method of substances for improving blood sugar level reduction effect of small red beans - Google Patents
Determination method of substances for improving blood sugar level reduction effect of small red beans Download PDFInfo
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Abstract
The invention discloses a determination method of substances for improving the blood sugar level reduction effect of small red beans, and relates to a determination method of substances. The method comprises the following steps: (1) purifying protein and polysaccharide of small red beans which are not extruded: A. crushing, and screening; B. purifying protein; C. purifying crude polysaccharide; (2) purifying protein and a polysaccharide liquid of small red beans which are not extruded: A. crushing and screening; B. adding water and stirring; C. extruding; D. crushing and screening; E. purifying protein; F. purifying crude polysaccharide; (3) comparing inhibitory activity of protein and polysaccharide to alpha glycosidase before and after extrusion by utilizing a method for determining glycosidase consistence in vitro. By comparing the substance compositional variation before and after extrusion, the problem that the content of protein and polysaccharide which are extruded increase obviously can be found easily. The glycosidase inhibition ratio of extruded and un-extruded small red bean powder can be determined easily by utilizing the method for determining glycosidase consistence in vitro.
Description
Technical field
The present invention relates to definite method of material, particularly, the present invention relates to a kind of definite method of the material that improves red bean blood sugar decreasing effect.
Background technology
In the past, red bean, due to its high fiber and protein content, is proposed as hypoglycemic food.Vegetalization scholar analyzes chemical composition in red bean, finds that it is rich in phenolic compound, catechin, chlorogenic acid etc.Red bean has alpha-glucosidase and suppresses active, and the blood glucose in diabetic rats of Streptozotocin (STZ) induction is improved to effect.Red bean juice can reduce triglyceride and suppress pancreatic lipase activity, is conducive to prevent and treat hypertriglyceridemia.In health functional food field, relatively less to the further investigation exploitation of red bean, especially red bean powder is played the middle crucial bioactivator shortage research of blood sugar reducing function.
Summary of the invention
The object of this invention is to provide a kind of definite method of the material that improves red bean blood sugar decreasing effect.The pharmacological results shows, red bean powder polysaccharide and the protein content by extrusion, prepared are higher, and effect of lowering blood sugar is better.
The present invention is that the technical scheme that solves the problems of the technologies described above employing is: a kind of definite method of the material that improves red bean blood sugar decreasing effect, comprises the following steps:
(1) do not pass through the albumen of red bean and the purifying of polysaccharide of extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, sieve, measure the composition of the material obtaining;
B, protein purification: adopt conventional soda acid sedimentation to carry out protein purification;
C, the conventional purifying of thick polysaccharide:
A, thick polysaccharide liquid preparation;
B, thick polysaccharide purification;
(2) albumen and the polysaccharide liquid purifying of the red bean of process extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, sieve;
B, add water and stir: in red bean powder, add the water of 8-10%, stir with stirring machine;
C, extrusion: get the red bean powder after stirring, put into extruder and carry out extrusion;
D, pulverizing, screening: the red bean powder after extrusion is pulverized, sieved, measure the composition of the material obtaining, relatively the material composition before and after extrusion changes;
E, protein purification: adopt conventional soda acid sedimentation to carry out protein purification;
F, the conventional purifying of thick polysaccharide:
A, thick polysaccharide liquid preparation;
B, thick polysaccharide purification;
(3) utilize the consistent method of external test glycosidase relatively to pass through albumen and the inhibition activity of polysaccharide to α glycosidase of extrusion expansion front and back.
Described sieving as crossing 55-65 mesh sieve.
The specific practice of described soda acid sedimentation of carrying out protein purification is: take material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 5-15min of 3500r, collecting supernatant 1M NaOH adjusts pH to 8.0-10.0, magnetic agitation 25-35min, the centrifugal 15-25min of 5000r, collects supernatant.With 1N hydrochloric acid adjust pH to 6.0-8.0, the centrifugal 15-25min of 5000r, abandoning supernatant, precipitate with deionized water rinse 2-3 time, freeze-drying is standby.
Specific practice prepared by described thick polysaccharide liquid is: take raw meal, soak 20-40min, add 15-25 times of water gaging ultrasonic dissolution bean powder, solution adds absolute ethyl alcohol to be made to reach 70-90% containing alcohol amount, and 3-5 ℃ of refrigeration is spent the night, the centrifugal 15-25min of 3500r, precipitation adds the water-soluble solution of 5-15mL, obtains thick polysaccharide liquid.
The specific practice of described thick polysaccharide purification is: thick polysaccharide liquid adds 10% trichloroacetic acid solution 0.5-1.5mL, 3-5 ℃ of refrigeration is spent the night, and the centrifugal 15-25min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 70-90% containing alcohol amount again, 3-5 ℃ of refrigeration is spent the night, the centrifugal 5-10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 5-15mL and adds 0.5% activated charcoal again, boiling water boiling 25-35min, filters, and adding distil water constant volume is in 25mL volumetric flask.
In described extrusion pressing puffing process, extruder I district temperature range is that 140-180 ℃, II district temperature range are 120-160 ℃.
The invention has the beneficial effects as follows:
1, by the relatively material composition variation of extrusion front and back, be very easy to find through albumen and the polyoses content of extrusion expansion and significantly increase.The albumen of the red bean of process extrusion expansion has the consistent activity of obviously higher α glycosidase with polysaccharide.
2, utilize the consistent method of external test glycosidase, can easily measure red bean powder and the glycosidase inhibiting rate that does not pass through extrusion red bean powder through extrusion, result shows can improve glycosidase inhibiting rate more than 4 times.
Accompanying drawing explanation
Fig. 1 is for extrusion expansion red bean powder is to normal mice hypoglycemic activity experimental result picture
Fig. 2 is that extrusion expansion red bean powder is to diabetic mice hypoglycemic activity experimental result picture
Fig. 3 is that extrusion expansion red bean albumen is to diabetic mice hypoglycemic activity experimental result picture
Fig. 4 is that extrusion expansion red bean albumen affects result figure to diabetic mice oral glucose tolerance
Embodiment
Embodiment 1:
A definite method that improves the material of red bean blood sugar decreasing effect, comprises the following steps:
(1) do not pass through the albumen of red bean and the purifying of polysaccharide of extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 55 mesh sieves, measure the composition of the material obtaining;
B, protein purification: adopt soda acid sedimentation to carry out protein purification: take material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 5min of 3500r, collects supernatant 1M NaOH and adjusts pH to 8.0, magnetic agitation 25min, the centrifugal 15min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 6.0, the centrifugal 15min of 5000r, abandoning supernatant, precipitate with deionized water rinse 2 times, freeze-drying is standby;
C, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 20min, add 15 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 70%, 3 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 15min of 3500r, precipitation adds the water-soluble solution of 5-15mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification: thick polysaccharide liquid adds 10% trichloroacetic acid solution 0.5mL, 3 ℃ of refrigerations are spent the night, and the centrifugal 15min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 70% containing alcohol amount again, 3 ℃ of refrigerations are spent the night, the centrifugal 5min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 5mL and adds 0.5% activated charcoal again, boiling water boiling 25min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(2) albumen and the polysaccharide liquid purifying of the red bean of process extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 55 mesh sieves;
B, add water and stir: in red bean powder, add 8% water, stir with stirring machine;
C, extrusion: get the red bean powder after stirring, put into extruder and carry out extrusion; I district temperature is that 140 ℃, II district temperature are 120 ℃;
D, pulverizing, screening: 55 mesh sieves are pulverized, crossed to the red bean powder after extrusion, measure the composition of the material obtaining, relatively the material composition before and after extrusion changes;
E, protein purification: adopt soda acid sedimentation to carry out protein purification: take a certain amount of material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 5min of 3500r, collects supernatant 1M NaOH and adjusts pH to 8.0, magnetic agitation 25min, the centrifugal 15min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 6.0, the centrifugal 15min of 5000r, abandoning supernatant, precipitate with deionized water rinse 2 times, freeze-drying is standby;
F, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 20min, add 15 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 70%, 3 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 15min of 3500r, precipitation adds the water-soluble solution of 5mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification; Thick polysaccharide liquid adds 10% trichloroacetic acid solution 0.5mL, 3 ℃ of refrigerations are spent the night, and the centrifugal 15min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 70% containing alcohol amount again, 3 ℃ of refrigerations are spent the night, the centrifugal 5min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 5mL and adds 0.5% activated charcoal again, boiling water boiling 25min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(3) utilize the consistent method of external test glycosidase relatively to pass through albumen and the inhibition activity of polysaccharide to α glycosidase of extrusion expansion front and back.
Embodiment 2:
A definite method that improves the material of red bean blood sugar decreasing effect, comprises the following steps:
(1) do not pass through the albumen of red bean and the purifying of polysaccharide of extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 60 mesh sieves, measure the composition of the material obtaining;
B, protein purification: adopt soda acid sedimentation to carry out protein purification: take material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 10min of 3500r, collects supernatant 1M NaOH and adjusts pH to 9, magnetic agitation 30min, the centrifugal 20min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 7.0, the centrifugal 20min of 5000r, abandoning supernatant, precipitate with deionized water rinse 2 times, freeze-drying is standby;
C, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 30min, add 20 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 80%, 4 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 20min of 3500r, precipitation adds the water-soluble solution of 10mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification: thick polysaccharide liquid adds 10% trichloroacetic acid solution 1.0mL, 4 ℃ of refrigerations are spent the night, and the centrifugal 20min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 80% containing alcohol amount again, 4 ℃ of refrigerations are spent the night, the centrifugal 10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 10mL and adds 0.5% activated charcoal again, boiling water boiling 30min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(2) albumen and the polysaccharide liquid purifying of the red bean of process extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 60 mesh sieves;
B, add water and stir: in red bean powder, add 9% water, stir with stirring machine;
C, extrusion: get the red bean powder after stirring, put into extruder and carry out extrusion; I district temperature is that 160 ℃, II district temperature are 140 ℃;
D, pulverizing, screening: 60 mesh sieves are pulverized, crossed to the red bean powder after extrusion, measure the composition of the material obtaining, relatively the material composition before and after extrusion changes;
E, protein purification: adopt soda acid sedimentation to carry out protein purification: take a certain amount of material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 10min of 3500r, collects supernatant 1M NaOH and adjusts pH to 9.0, magnetic agitation 30min, the centrifugal 20min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 7.0, the centrifugal 20min of 5000r, abandoning supernatant, precipitate with deionized water rinse 3 times, freeze-drying is standby;
F, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 30min, add 20 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 80%, 4 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 20min of 3500r, precipitation adds the water-soluble solution of 10mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification; Thick polysaccharide liquid adds 10% trichloroacetic acid solution 1.0mL, 4 ℃ of refrigerations are spent the night, and the centrifugal 20min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 80% containing alcohol amount again, 4 ℃ of refrigerations are spent the night, the centrifugal 10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 10mL and adds 0.5% activated charcoal again, boiling water boiling 30min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(3) utilize the consistent method of external test glycosidase relatively to pass through albumen and the inhibition activity of polysaccharide to α glycosidase of extrusion expansion front and back.
Embodiment 3:
A definite method that improves the material of red bean blood sugar decreasing effect, comprises the following steps:
(1) do not pass through the albumen of red bean and the purifying of polysaccharide of extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 65 mesh sieves, measure the composition of the material obtaining;
B, protein purification: adopt soda acid sedimentation to carry out protein purification: take material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 15min of 3500r, collects supernatant 1M NaOH and adjusts pH to 10.0, magnetic agitation 35min, the centrifugal 25min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 8.0, the centrifugal 25min of 5000r, abandoning supernatant, precipitate with deionized water rinse 3 times, freeze-drying is standby;
C, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 40min, add 25 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 90%, 5 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 25min of 3500r, precipitation adds the water-soluble solution of 15mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification: thick polysaccharide liquid adds 10% trichloroacetic acid solution 1.5mL, 5 ℃ of refrigerations are spent the night, and the centrifugal 25min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 90% containing alcohol amount again, 5 ℃ of refrigerations are spent the night, the centrifugal 10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 15mL and adds 0.5% activated charcoal again, boiling water boiling 35min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(2) albumen and the polysaccharide liquid purifying of the red bean of process extrusion:
A, pulverizing, screening: get up-to-standard red bean, pulverize, cross 65 mesh sieves;
B, add water and stir: in red bean powder, add 10% water, stir with stirring machine;
C, extrusion: get the red bean powder after stirring, put into extruder and carry out extrusion; I district temperature is that 180 ℃, II district temperature are 160 ℃;
D, pulverizing, screening: 65 mesh sieves are pulverized, crossed to the red bean powder after extrusion, measure the composition of the material obtaining, relatively the material composition before and after extrusion changes;
E, protein purification: adopt soda acid sedimentation to carry out protein purification: take a certain amount of material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 15min of 3500r, collects supernatant 1M NaOH and adjusts pH to 10.0, magnetic agitation 35min, the centrifugal 25min of 5000r, collects supernatant.With 1N hydrochloric acid tune pH to 8.0, the centrifugal 25min of 5000r, abandoning supernatant, precipitate with deionized water rinse 3 times, freeze-drying is standby;
F, thick polysaccharide purification:
A, the preparation of thick polysaccharide liquid: take raw meal, soak 40min, add 25 times of water gaging ultrasonic dissolution bean powderes, solution adds absolute ethyl alcohol to be made to reach 90%, 5 ℃ of refrigeration containing alcohol amount and spend the night, the centrifugal 25min of 3500r, precipitation adds the water-soluble solution of 15mL, obtains thick polysaccharide liquid;
B, thick polysaccharide purification; Thick polysaccharide liquid adds 10% trichloroacetic acid solution 1.5mL, 5 ℃ of refrigerations are spent the night, and the centrifugal 25min of 3500r, gets supernatant, adding absolute ethyl alcohol makes to reach 90% containing alcohol amount again, 5 ℃ of refrigerations are spent the night, the centrifugal 10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 15mL and adds 0.5% activated charcoal again, boiling water boiling 35min, filters, and adding distil water constant volume is in 25mL volumetric flask;
(3) utilize the consistent method of external test glycosidase relatively to pass through albumen and the inhibition activity of polysaccharide to α glycosidase of extrusion expansion front and back.
Below by experimental technique, illustrate beneficial effect of the present invention:
Experiment 1: glycosidase is measured experiment in vitro, utilizes chemistry in detecting, selects glucose assays kit, measures the glycosidase inhibiting rate of the red bean powder of different cultivars, wherein with the positive contrast of hypoglycemic Acarbose, and experimental result replicate determination 2 times.
Result is as follows: different cultivars red bean glycosidase inhibiting rate:
| Numbering | Variety name | Inhibiting |
| 1 | Lin County red bean | 43.63 |
| 2 | Red rich No. 8 | 42.52 |
| 3 | Guanling | 41.79 |
| 4 | Ji red 352 | 46.67 |
| 5 | In red 2 | 45.27 |
| 6 | In red 6 | 41.66 |
| 7 | In red 5 | 37.66 |
| 8 | In red 8 | 42.09 |
| 9 | In red 9 | 40.86 |
This results show: red 352 glucoside inhibiting activities in Ji that concentration is 100mg/ml are the highest, are chosen as hypoglycemic kind.
Experiment 2: the process optimization of extrusion
| Numbering | Temperature variation | Glycosidase inhibiting |
| 1 | 150-160 | 60.94 |
| 2 | 150-165 | 64.76 |
| 3 | 150-170 | 60.47 |
| 4 | 155-160 | 62.84 |
| 5 | 155-165 | 60.65 |
| 6 | 155-170 | 65.09 |
| 7 | 160-160 | 61.92 |
| 8 | 160-165 | 62.89 |
| 9 | 160-170 | 58.30 |
This experimental result shows: under temperature variation 155-175 degree, the glucoside inhibiting activity of the red bean of process extrusion is the highest.
Experiment 3: the component content of the expanded front and back of red bean powder active substance changes, antioxidation activity and the experiment of α glucoside inhibiting activity change detection.Half inhibiting rate/IC50 concentration of the water extraction liquid of the expanded front and back of little bean powder of experimental calculation 20mg/ml:
This results show: after extrusion red bean than extrusion expansion before glycosidase inhibiting rate significantly improve.
Experiment 4: the impact of its glycosidase inhibiting rate before and after extrusion red bean powder adds:
| ? | α glycosidase inhibiting rate (%) |
| Do not push red bean powder polysaccharide | 6.33±0.12c |
| Extruding red bean powder polysaccharide | 35.47±2.74b |
| Do not push red bean amyloid proteins | 1.66±0.05c |
| Extruding red bean amyloid proteins | 60.44±5.68a |
This results show: polysaccharide and the albumen of the red bean powder of process extrusion expansion are respectively 35.47% and 60.44% to the inhibiting rate of alpha-glucosidase, illustrates that through red bean powder polysaccharide and the albumen of extrusion expansion be hypoglycemic principal ingredient.
Experiment 5: extrusion expansion red bean powder hypoglycemic activity research: zoopery, before formal experiment, all animal fasting be can't help to water for 14 hours, hyperglycaemia mouse and the normal SD mouse of STZ induction are divided into respectively to 4 groups: gavage water does not process---control group; Gavage 200mg/kg red bean extracts---ABE group; Gavage 200mg/kg extruding red bean extracts---EABE group; Gavage 50mg/kg acarbose group, the 30min sucrose solution of gavage 2g/kg again after gavage, measure its after gavage sucrose 0,30,60, the blood glucose value of 120min, blood sugar detection adopts tail venous blood sampling, experimental result respectively as depicted in figs. 1 and 2.
Experimental result shows: through the red bean of extrusion expansion, can suppress the rising of normal mice and diabetic mice postprandial blood sugar, improve Glucose Tolerance.
Experiment 6: extrusion expansion red bean albumen hypoglycemic activity research.Animal used as test: 30 genotype diabetes KK-Ay mouse, the high glucose and high fat feed of feeding, and raise and within the 3rd day, the 7th day, survey respectively blood sugar in adaptability, getting KK-Ay mouse blood sugar value is greater than 11.1mol/L and includes experiment in, and be divided at random diabetic model group, high protein dosage group by blood glucose value---2% and low albumen dosage group that albumen addition is feed---albumen addition is feed 1%, feed continuously 6 weeks.
Through the red bean albumen of extrusion expansion on diabetic mice blood sugar, affect result as shown in Figure 3.
Experimental result shows: feed behind the red bean protein 42 sky of extrusion expansion, through the each dosage group of red bean albumen of extrusion expansion, all can obviously reduce diabetic mice blood sugar level.
Experiment 7: diabetic mice oral glucose tolerance is affected to result as shown in Figure 4 through extrusion expansion red bean albumen.
The results show: the red bean albumen of feeding through extrusion expansion is after six weeks, and diabetic mice Glucose Tolerance obviously improves, model group reaches peak value giving blood sugar after syrup 30min, and still keeps higher level after 30min.Push the each dosage group of little legumin after 30 minutes blood glucose value all lower than model group.
Claims (6)
1. a definite method that improves the material of red bean blood sugar decreasing effect, comprises the following steps:
(1) do not pass through the albumen of red bean and the purifying of polysaccharide of extrusion:
A, pulverizing, screening: get red bean, pulverize, sieve, measure the composition of the material obtaining;
B, protein purification: adopt conventional soda acid sedimentation to carry out protein purification;
C, the conventional purifying of thick polysaccharide:
A, thick polysaccharide liquid preparation;
B, thick polysaccharide purification;
(2) albumen and the polysaccharide liquid purifying of the red bean of process extrusion:
A, pulverizing, screening: separately get red bean, pulverize, sieve, measure the composition of the material obtaining;
B, add water stir: in red bean powder, add the water of 8-10%, stir;
C, extrusion: get the red bean powder after stirring, carry out extrusion;
D, pulverizing, screening: the red bean powder after extrusion is pulverized, sieved;
E, protein purification: adopt conventional soda acid sedimentation to carry out protein purification;
F, the conventional purifying of thick polysaccharide:
A, thick polysaccharide liquid preparation;
B, thick polysaccharide purification;
(3) utilize the consistent method of external test glycosidase relatively to pass through albumen and the inhibition activity of polysaccharide to α glycosidase of extrusion expansion front and back.
2. definite method of the material of raising red bean blood sugar decreasing effect according to claim 1, described in sieve as crossing 55-65 mesh sieve.
3. definite method of the material of raising red bean blood sugar decreasing effect according to claim 1, the specific practice of described soda acid sedimentation of carrying out protein purification is: take material powder distilled water according to 1:8 ratio soaked overnight, the centrifugal 5-15min of 3500r, collecting supernatant 1M NaOH adjusts pH to 8.0-10.0, magnetic agitation 25-35min, the centrifugal 15-25min of 5000r, collects supernatant.With 1N hydrochloric acid adjust pH to 6.0-8.0, the centrifugal 15-25min of 5000r, abandoning supernatant, precipitate with deionized water rinse 2-3 time, freeze-drying is standby.
4. definite method of the material of raising red bean blood sugar decreasing effect according to claim 1, specific practice prepared by described thick polysaccharide liquid is: take raw meal, soak 20-40min, add 15-25 times of water gaging ultrasonic dissolution bean powder, solution adds absolute ethyl alcohol to be made to reach 70-90% containing alcohol amount, and 3-5 ℃ of refrigeration is spent the night, the centrifugal 15-25min of 3500r, precipitation adds the water-soluble solution of 5-15mL, obtains thick polysaccharide liquid.
5. definite method of the material of raising red bean blood sugar decreasing effect according to claim 1, the specific practice of described thick polysaccharide purification is: thick polysaccharide liquid adds 10% trichloroacetic acid solution 0.5-1.5mL, 3-5 ℃ of refrigeration is spent the night, the centrifugal 15-25min of 3500r, get supernatant, adding absolute ethyl alcohol makes to reach 70-90% containing alcohol amount again, 3-5 ℃ of refrigeration is spent the night, the centrifugal 5-10min of 4500r, abandoning supernatant, precipitation adds the water-soluble solution of 5-15mL and adds 0.5% activated charcoal, boiling water boiling 25-35min again, filter, adding distil water constant volume is in 25mL volumetric flask.
6. definite method of the material of raising red bean blood sugar decreasing effect according to claim 1, the extruder I district temperature range in described extrusion pressing puffing process is that 140-180 ℃, II district temperature range are 120-160 ℃.
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