CN103735961B - The application in preparation suppression lymphoma cell YAC-1 cell proliferation of the Herba Boenninghauseniae cough with asthma sheet - Google Patents
The application in preparation suppression lymphoma cell YAC-1 cell proliferation of the Herba Boenninghauseniae cough with asthma sheet Download PDFInfo
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- 208000006673 asthma Diseases 0.000 title claims abstract description 45
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- 230000001629 suppression Effects 0.000 title claims abstract description 16
- 206010025323 Lymphomas Diseases 0.000 title claims abstract description 13
- 230000004663 cell proliferation Effects 0.000 title claims abstract description 12
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- 229940079593 drug Drugs 0.000 claims abstract description 16
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims abstract description 15
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- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 abstract description 12
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- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 abstract description 12
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- 229940126678 chinese medicines Drugs 0.000 abstract description 2
- 229960004756 ethanol Drugs 0.000 description 25
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- 238000012360 testing method Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
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- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
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- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention belongs to technical field of Chinese medicines, be specifically related to the application in preparation suppression lymphoma cell YAC 1 cell proliferation of a kind of Herba Boenninghauseniae cough with asthma sheet and the preparation method of Herba Boenninghauseniae cough with asthma sheet.The application in preparation suppression lymphoma cell YAC 1 cell proliferation of the Herba Boenninghauseniae cough with asthma sheet of the present invention, described Herba Boenninghauseniae cough with asthma sheet is made up as crude drug of Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae 750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g, supercritical extraction and microwave extracting is used to be prepared from so that content of hesperidin improves a lot.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of Herba Boenninghauseniae cough with asthma sheet thin at preparation suppression lymphoma cell YAC-1
Application in born of the same parents' hyperproliferation agent and the preparation method of Herba Boenninghauseniae cough with asthma sheet.
Background technology
Herba Boenninghauseniae cough with asthma particulate level WS-10449(ZD-0449)-2002, it is recorded in country's standard for traditional Chinese medicines compilation internal medicine
Taste fascicle.By Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, lead to
Pass rattan 750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g make as crude drug, have clear
Transconversion into heat expectorant, effect of relieving cough and asthma.The cough expectorant caused for lung-heat is thick, xerostomia itching throat, and acute/chronic bronchitis causes
Phlegm-damp cough with asthma.
In prior art, not yet there is Herba Boenninghauseniae cough with asthma granule in preparation suppression lymphoma cell YAC-1 cell proliferation
The report of application, also there are no Herba Boenninghauseniae cough with asthma granule and extracts preparation aspect employing supercritical and the report of microwave technology, and volatilize
Oil extracts, the method for soak by water, and technique is coarse, backward, and impurity is many, causes patient's consumption excessive, it has not been convenient to take, seriously
Have impact on this product to apply clinically.
Summary of the invention
Goal of the invention: it is an object of the invention to provide a kind of Herba Boenninghauseniae cough with asthma sheet at preparation suppression lymphoma cell YAC-1 cell
Application in hyperproliferation agent.
Further object is that the preparation method that a kind of Herba Boenninghauseniae cough with asthma sheet is provided.
It is an object of the invention to by following scheme realization:
The application in preparation suppression lymphoma cell YAC-1 cell proliferation of the Herba Boenninghauseniae cough with asthma sheet, described Herba Boenninghauseniae cough with asthma sheet
By Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae 750g,
Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g make as crude drug, described Herba Boenninghauseniae cough with asthma sheet
Preparation method be made up of the following step: take Rhizoma Acori Graminei, Herba laggera, Herba Boenninghauseniae, Herba Houttuyniae, join CO2Supercritical extraction
In device, ethanol is as entrainer, and it is 4-6% that entrainer accounts for the percent by volume of total extractant, extracting pressure 15-30MPa,
Temperature 30-50 DEG C, CO2Flow l-3ml/g crude drug min, extraction time 180-220min, obtain supercritical extract, standby
With；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put into and microwave extracting apparatus carries out microwave extracting, extract power
600-800W, extracts 2 times, each 5-10 minute, combining extraction liquid, concentrates, is added to Diaion HP-10 macroporous absorption tree
On fat post, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, be concentrated and dried, obtain microwave and carry
Take thing, standby；Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule, be dried,
Tabletting, makes 250, every tablet weight 0.5g.
Preferably, the application in preparation suppression lymphoma cell YAC-1 cell proliferation of the above-mentioned Herba Boenninghauseniae cough with asthma sheet, institute
State Herba Boenninghauseniae cough with asthma sheet by Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, the Radix Stemonae
96g, Caulis Marsdeniae Tenacissimae 750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g are as crude drug system
Becoming, the preparation method of described Herba Boenninghauseniae cough with asthma sheet is made up of the following step: take Rhizoma Acori Graminei, Herba laggera, Herba Boenninghauseniae, Herba Houttuyniae,
Join CO2In supercritical extraction device, ethanol is as entrainer, and it is 5% that entrainer accounts for the percent by volume of total extractant, extraction
Pressure power 15-30MPa, temperature 30-50 DEG C, CO2Flow l-3ml/g crude drug min, extraction time 200min, obtain supercritical
Extract, standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put in microwave extracting apparatus and carry out microwave extraction
Take, extract power 700W, extract 2 times, each 5-10 minute, combining extraction liquid, concentration, it is added to Diaion HP-10 big
On macroporous adsorbent resin post, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, be concentrated and dried,
Obtain microwave extraction thing, standby；Above-mentioned supercritical extract and microwave extraction thing are mixed, adds starch, 70% ethanol system
Grain, is dried, tabletting, makes 250, every tablet weight 0.5g.
In prior art, each 8-16g of Herba Boenninghauseniae cough with asthma granule, 3-4 time on the one.Herba Boenninghauseniae cough with asthma granule dosage is big, and
Granule preparation needs to add substantial amounts of sugar, and diabetics is not suitable for, and if not sugaring, taste is the best, and patient is not easy under clothes,
Compliance is poor.Herba Boenninghauseniae every tablet weight 0.5g of cough with asthma sheet using the inventive method to be prepared as, the most only needs 2-4 sheet, clothes on the one
With 3-4 time.Dose is greatly reduced under conditions of there is more active component.This conclusion can be by following test card
Bright.And it being prepared as tablet, patient swallows with water, and dosage is little and without bitterness, and patient is prone to accept.
The comparison of content of hesperidin in Herba Boenninghauseniae cough with asthma sheet prepared by test one, distinct methods
L, instrument and reagent Herba Boenninghauseniae of the present invention cough with asthma sheet: prepare by embodiment 1 method, uses 3007g crude drug, through carrying
Take and make 250, every tablet weight 0.5g.Former Herba Boenninghauseniae cough with asthma granule, according to WS-10449(ZD-0449)-2002 standard sides
Prepared by method.Agilent1200 high performance liquid chromatograph；METTLER AE240 electronic analytical balance；Hesperidin reference substance (in
State's medicine biological products assay institute).
2, method
Measure according to high performance liquid chromatography (Chinese Pharmacopoeia one annex VI D of version in 2010).
Chromatographic condition and system suitability are with octadecylsilane chemically bonded silica as filler；Acetonitrile-0.1% phosphoric acid solution
(24: 76) are flowing phase, and detection wavelength is 283nm.Number of theoretical plate is calculated by Hesperidin peak should be not less than 4000.
It is appropriate that the preparation of reference substance solution takes Hesperidin reference substance, accurately weighed, adds methanol and makes molten containing 60 μ g of every 1ml
Liquid, to obtain final product.
The preparation of need testing solution takes the Herba Boenninghauseniae cough with asthma sheet of the present invention, finely ground, takes 0.25g, accurately weighed, puts conical flask
In, accurate addition methanol 25ml, weighed weight, it is heated to reflux 30 minutes, lets cool, more weighed weight, supply with methanol and subtract
Weight loss, filters, takes subsequent filtrate, to obtain final product.
The preparation of reference product need testing solution takes the Herba Boenninghauseniae cough with asthma granule of this comparison, finely ground, takes 4g, accurately weighed,
Put in conical flask, accurate addition methanol 25ml, weighed weight, it is heated to reflux 30 minutes, lets cool, more weighed weight, use first
Less loss weight supplied by alcohol, filters, takes subsequent filtrate, to obtain final product.
The preparation of need testing solution takes the content under this product content uniformity item, finely ground, mixing, and algoscopy precision respectively is inhaled
Take reference substance solution and each 5 μ l of need testing solution, inject chromatograph of liquid, measure, to obtain final product
3, result
Result shows, in Herba Boenninghauseniae cough with asthma sheet of the present invention, the content of Hesperidin is 15-30mg/ sheet；And former Herba Boenninghauseniae cough with asthma granule
The content of middle Hesperidin is 3.2mg/ bag, each serving 5-10 times that content of hesperidin is former granule content of consumption 2,
In the case of dose reduces, content of hesperidin improves a lot.
The studies above shows, uses Herba Boenninghauseniae cough with asthma sheet prepared by preparation method of the present invention, and active constituent content is significantly larger than WS-
10449(ZD-0449) Herba Boenninghauseniae cough with asthma granule prepared by the method that-2002 standards are recorded.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, in order to those skilled in the art knows more about this
Bright, but this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to Examples below, all based on foregoing institute of the present invention
The technology realized belongs to the scope of the present invention.
Embodiment 1
Take Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae
750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g, by Rhizoma Acori Graminei, Herba laggera, stone green pepper
Grass, Herba Houttuyniae join CO2In supercritical extraction device, ethanol is as entrainer, and entrainer accounts for the volume basis of total extractant
Ratio is 5%, extracting pressure 25MPa, temperature 40 DEG C, CO2Flow 2ml/g crude drug min, extraction time 200min, must surpass and face
Boundary's extract, standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put in microwave extracting apparatus and carry out microwave extraction
Take, extract power 700W, extract 2 times, each 8 minutes, combining extraction liquid, concentration, it is added to Diaion HP-10 macropore
On adsorption resin column, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, be concentrated and dried,
Microwave extraction thing, standby；Above-mentioned supercritical extract and microwave extraction thing are mixed, addition starch, 70% ethanol granule,
It is dried, tabletting, makes 250, every tablet weight 0.5g.
After testing, in finished product, the content of Hesperidin is 59.6mg/ sheet.
Embodiment 2
Take Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae
750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g, by Rhizoma Acori Graminei, Herba laggera, stone green pepper
Grass, Herba Houttuyniae join CO2In supercritical extraction device, ethanol is as entrainer, and entrainer accounts for the volume basis of total extractant
Ratio is 4%, extracting pressure 30MPa, temperature 50 C, CO2Flow 3ml/g crude drug min, extraction time 180min, must surpass and face
Boundary's extract, standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put in microwave extracting apparatus and carry out microwave extraction
Take, extract power 600W, extract 2 times, each 5 minutes, combining extraction liquid, concentration, it is added to Diaion HP-10 macropore
On adsorption resin column, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, be concentrated and dried,
Microwave extraction thing, standby；Above-mentioned supercritical extract and microwave extraction thing are mixed, addition starch, 70% ethanol granule,
It is dried, tabletting, makes 250, every tablet weight 0.5g.
After testing, in finished product, the content of Hesperidin is 32.9mg/ sheet.
Embodiment 3
Take Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae
750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g, by Rhizoma Acori Graminei, Herba laggera, stone green pepper
Grass, Herba Houttuyniae join CO2In supercritical extraction device, ethanol is as entrainer, and entrainer accounts for the volume basis of total extractant
Ratio is 6%, extracting pressure 15MPa, temperature 30 DEG C, CO2Flow 1ml/g crude drug min, extraction time 220min, must surpass and face
Boundary's extract, standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put in microwave extracting apparatus and carry out microwave extraction
Take, extract power 800W, extract 2 times, each 10 minutes, combining extraction liquid, concentration, it is added to Diaion HP-10 macropore
On adsorption resin column, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, be concentrated and dried,
Microwave extraction thing, standby；Above-mentioned supercritical extract and microwave extraction thing are mixed, addition starch, 70% ethanol granule,
It is dried, tabletting, makes 250, every tablet weight 0.5g.
After testing, in finished product, the content of Hesperidin is 44.7mg/ sheet.
Embodiment 4: the experimentation data of Herba Boenninghauseniae cough with asthma sheet suppression mouse lymphoma cell YAC-1 cell proliferation
1. experiment material
1.1 experiment cell strains
Mouse lymphoma cell YAC-1 cell, Shandong University's laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: Herba Boenninghauseniae cough with asthma sheet of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: weigh 100mg Herba Boenninghauseniae cough with asthma sheet, be dissolved in 5ml dehydrated alcohol, 0.2 μm filter filters,
500 μ ldoff pipe subpackages ,-20 DEG C of storages, 0.2 μm filter filters dehydrated alcohol in case matched group is used simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137)；Hyclone (Hangzhoupro, sky, Zhejiang biotechnology
Company limited Lot.No.100419)；NaHC03(the long hundred million chemical reagent company limited Cat.No.11810-033Lot.No. in Shanghai
1088387)；Trypsin(AMRESCO company)；EDTA(AMRESCO company)；Penicillin G Sodium Salt
(AMRESCO company 1)；Streptomycin Sulfate(AMRESCO)；Dehydrated alcohol (the limited public affairs of Zibo Ya Dulan economy and trade
Department)；MTT (Biosharp lot number: 0793): PBS(laboratory autogamy)；
1.4 experiment equipment
Lycra inverted microscope (Germany Leica model: DMIL)；Visible-ultraviolet light microwell plate detector (MD company type of the U.S.
Number: SPECTRAMAX190)；C02Incubator (FORMA model: 3111)；Super-clean bench (safe and sound company of Su Jing group manufacture type
Number: SW-CJ-ZFD)；Pure water instrument (Sprlng company of U.S. model: S/N020579)；(France's gill is gloomy for accurate pipettor
Company's model: P2)；Electronic balance (Sai Duolisi company limited of Germany model: BT323S)；Full-automatic high-pressure autoclave (day
This SANYO company model: MLS-3020)；Table electrothermal air dry oven (Shanghai precision experimental facilities company model:
DHG9123A)；Refrigerator (Siemens Company's model: KG18V21TI)；Liquid nitrogen container (CBS model: 2001)；Low speed centrifuge
(Anting Scientific Instrument Factory, Shanghai's model: KA-1000)；0.2 μm filter (MILLIPORE model: SLGP033RB)；1cm trains
Support ware (NEST company), 96 well culture plates (NEST company)；Cell counting count board；Centrifuge tube, pipet, Tips are some.
2. experimental technique
1) YAC-1 cell DMEM+10%FBS in 37 DEG C, 5%C02 carry out cellar culture (10cm culture dish), when cell grow
During to logarithmic (log) phase, collecting cell, discard culture fluid, PBS rinses 3 times, and addition 3ml0.25% trypsin-
0.04%EDTA, after 37 DEG C of digestion 2min, is added thereto in 5ml complete medium and reacts, being proceeded to after piping and druming cell
In centrifuge tube, 1000rpm is centrifuged 5min, adjusts concentration of cell suspension 3 × 104Individual/ml.
2) entering in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate is put in cell culture incubator
(37 DEG C, 5%C02) cellar culture.
3) according to cell growth status, general long to 50%-70%, add Herba Boenninghauseniae cough with asthma sheet solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole such as enters 200 μ l dimethyl sulfoxide, puts on shaking table
Low-speed oscillation 10min, makes crystal fully dissolve.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm.
6) arranging background (being not added with cell, only add culture fluid), (cell, the medicine dissolution of same concentrations are situated between control wells simultaneously
Matter, culture fluid, MTT, dimethyl sulfoxide), often group sets 6 multiple holes.
7) suppression ratio of cell is represented by result with medicine: cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD
Value), control wells OD value × 100%.Experiment is repeated 3 times.
3. statistical disposition
Using the correlation analysis in Microsoft Excel2007 software and Student t inspection, data are with mean ± SD table
Show.
4. experimental result
Statistical result showed after mtt assay experiment, compares with matched group, when dosage reaches 5mg/ml, increases YAC-1 cell
Growing suppression variant (P < 0.05), dosage this difference when 10mg/ml has significance (P < 0.01), when dosage reaches 15-
Pole significant difference (P < 0.001) is had during 20mg/ml.
Table 1 Herba Boenninghauseniae cough with asthma sheet is to YAC-1 cell inhibitory effect influence research (X ± SD)
| Group | Drug level (mg/ml) | Suppression ratio (%) |
| Matched group | 0 | 0 |
| 1 | 5 | 10.31±7.66 |
| 2 | 10 | 18.69±9.71* |
| 3 | 15 | 28.34±12.96** |
| 4 | 20 | 39.64±14.03** |
Note: compare with matched group, * P is < O.01；**P<0.001.
5. experiment conclusion
The Herba Boenninghauseniae cough with asthma sheet of the present invention can suppress YAC-1 cell proliferation, reduces the cell growing number of YAC-1 cell, should
Effect is in dose dependent.
Claims (2)
1. the application in preparation suppression lymphoma cell YAC-1 cell proliferation of the Herba Boenninghauseniae cough with asthma sheet, described stone
Green pepper grass cough with asthma sheet by Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi 188g, Herba Boenninghauseniae 375g, hundred
Portion 96g, Caulis Marsdeniae Tenacissimae 750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, Herba Houttuyniae 375g, Cortex Mori 188g make
Make for crude drug, it is characterised in that the preparation method of described Herba Boenninghauseniae cough with asthma sheet is made up of the following step: take
Rhizoma Acori Graminei, Herba laggera, Herba Boenninghauseniae, Herba Houttuyniae, join CO2In supercritical extraction device, ethanol is as carrying secretly
Agent, it is 4-6% that entrainer accounts for the percent by volume of total extractant, extracting pressure 15-30MPa, temperature
30-50 DEG C, CO2Flow l-3ml/g crude drug min, extraction time 180-220min, obtain supercritical extract,
Standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put in microwave extracting apparatus and carry out microwave extraction
Take, extract power 600-800W, extract 2 times, each 5-10 minute, combining extraction liquid, concentration, it is added to
On Diaion HP-10 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, subtract
Push back receipts ethanol, be concentrated and dried, obtain microwave extraction thing, standby；Above-mentioned supercritical extract and microwave are carried
Take thing mixing, add starch, 70% ethanol granule, be dried, tabletting, make 250, every tablet weight 0.5g.
2. according to the Herba Boenninghauseniae cough with asthma sheet described in claim l at preparation suppression lymphoma cell YAC-1 cell proliferation
Application in medicine, described Herba Boenninghauseniae cough with asthma sheet is by Pericarpium Citri Reticulatae 156g, Rhizoma Acori Graminei 96g, Rhizoma Polygoni Cuspidati 312g, Radix Asparagi
188g, Herba Boenninghauseniae 375g, Radix Stemonae 96g, Caulis Marsdeniae Tenacissimae 750g, Herba laggera 375g, Semen Armeniacae Amarum 96g, fish raw meat
Grass 375g, Cortex Mori 188g make as crude drug, it is characterised in that the preparation of described Herba Boenninghauseniae cough with asthma sheet
Method is made up of the following step: takes Rhizoma Acori Graminei, Herba laggera, Herba Boenninghauseniae, Herba Houttuyniae, joins CO2Supercritical
In extractor, ethanol is as entrainer, and it is 5% that entrainer accounts for the percent by volume of total extractant, extraction pressure
Power 15-30MPa, temperature 30-50 DEG C, CO2Flow l-3ml/g crude drug min, extraction time 200min,
Supercritical extract, standby；Take remaining Chinese medicine, pulverize, add 50% ethanol of 5L, put into microwave extracting dress
Carry out microwave extracting in putting, extract power 700W, extract 2 times, each 5-10 minute, combining extraction liquid,
Concentrate, be added on Diaion HP-10 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volumes
Eluent, decompression recycling ethanol, it is concentrated and dried, obtains microwave extraction thing, standby；By above-mentioned supercritical extraction
Thing and the mixing of microwave extraction thing, add starch, 70% ethanol granule, be dried, tabletting, make 250,
Every tablet weight 0.5g.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628770A (en) * | 2004-08-27 | 2005-06-22 | 唐秋海 | Cough stopping and asthma relieving medicine and its preparation |
| CN101015645A (en) * | 2007-03-09 | 2007-08-15 | 阮玉江 | Medicine for treating cancer and preparing method therefor |
| CN102133363A (en) * | 2011-03-22 | 2011-07-27 | 唐秋海 | Quality detection method for chinarue cough particle |
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2013
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628770A (en) * | 2004-08-27 | 2005-06-22 | 唐秋海 | Cough stopping and asthma relieving medicine and its preparation |
| CN101015645A (en) * | 2007-03-09 | 2007-08-15 | 阮玉江 | Medicine for treating cancer and preparing method therefor |
| CN102133363A (en) * | 2011-03-22 | 2011-07-27 | 唐秋海 | Quality detection method for chinarue cough particle |
Non-Patent Citations (1)
| Title |
|---|
| 基于二苯乙烯类为主要活性成分的虎杖药理作用研究进展;贾玉梅等;《中国实验方剂学杂志》;20111231(第09期);263-269 * |
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