CN103734018A - Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling - Google Patents
Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling Download PDFInfo
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- CN103734018A CN103734018A CN201410031841.9A CN201410031841A CN103734018A CN 103734018 A CN103734018 A CN 103734018A CN 201410031841 A CN201410031841 A CN 201410031841A CN 103734018 A CN103734018 A CN 103734018A
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Abstract
The invention discloses a subculture medium for a saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling. The subculture medium comprises the following components: CaCl2.2H2O, NH4NO3, KNO3, NaCl, MgSO4, NaH2PO4.2H2O, K2SO4, Na2SO4, CaCl2, CoCl2.6H2O, CuSO4.5H2O, H3BO3, MnSO4.H2O, Na2MoO4.2H2O, ZnSO4.7H2O, KI, FeSO4.7H2O, Na2EDTA.2H2O, inositol, glycine, nicotinic acid, V6, V1, biotin, folic acid, riboflavin and vitamin. Compared with a traditional culture medium, the subculture medium disclosed by the invention improves the average reproduction rate of the ulmus pumila L. tissue culture seedling by more than 5 times, and improves the average reproduction coefficient by more than 2.3 times.
Description
Technical field
The present invention relates to the minimal medium of a kind of group of training seedling, particularly the minimal medium of a series of Salt And Alkali Tolerance fast-growing American elm group training seedling subcultures.
Background technology
American elm (Ulmus pumila L.), for Ulmaceae (Ulmaceae) Elm (Ulmus) plant, mutation is more, and elm is always one of large Resource of Indigenous Landscape Species in northern China city five [willow (Populus), willow (Salix), elm (Ulmus), Chinese scholartree (Sophora), tree-of-heaven (Ailanthus)], one of the Ye Shi world four large Shade Trees, have application comparatively widely.
American elm is the long indigenous tree species of China's cultivation history, because it has good, barren-resistant, the anti-feature such as saline and alkaline of drought-resistant, high temperature resistant, cold-resistant, fast-growing, material, is used for saline land and afforests with drought and barren area.American elm wood is tough and tensile, and texture is sensible clear, and hardness and moderate strength can be for furniture, finishings etc.American elm bark, blade, the equal edible of fruit are good medicinal materials.
Due to the sharply increase of population, industrialized high speed development, the application of unreasonable irrigation in addition, tillage method, make large area good farmland deteriorate to gradually no longer cultivated secondary salinization soil, caused the serious deterioration of the ecological environment, greatly limited the development of Saline-alkali Soil greening.American elm is as one of main native soil, gardens ornamental tree species of China, to toxic gas resistances such as flue dust, sulphur dioxide and hydrogen fluoride, and there is high environmental suitability, antipollution, Resistant, should breed and stronger Salt And Alkali Tolerance characteristic, make the American elm utmost point promise to be the key seeds of varieties in saline-alkali areas afforestation.
Successful experience with the case relative herbaceous plant of woody plant in Plant Tissue Breeding is produced is comparatively rare, and the scarcity of the basal medium of suitable different woody plant growths causes woody plant to be difficult to large-scale production.In addition, American elm vegetative propagation is mainly by grafting, and reproduction speed is slow, seriously restricts the development of American elm.Therefore,, for the very big exploitation value of American elm, urgently develop a kind of for American elm growth substrate basal culture medium.
At present American elm can with minimal medium have MS(Sun Zhen, Wang Jinghua, Hou Jiansheng, the minimal medium that the people such as Liu Guilin use the research institute of American elm group training seedling proliferation subculture), the woody pine genus plant production of DKW(minimal medium), WPM(M.P.Corchete, J.J.Diez, the minimal medium that the people such as and T.Valle. use the research institute of American elm group training seedling proliferation subculture).The appreciation rate of above-mentioned medium is low, the expensive and wasting of resources that causes American elm batch production to produce.
Summary of the invention
The object of the invention is to provide for woody plant American elm group training seedling the condition of culture of a suitable growth, on the basis of original all kinds of minimal mediums, invent the medium of new special suitable American elm growth.Reduction is selected the improper wasting of resources causing that American elm batch production is produced because of minimal medium, saves cost, enhances productivity.
Technical scheme of the present invention is: a kind of Salt And Alkali Tolerance fast-growing American elm group is trained seedling subculture medium (being called for short EM medium), comprises the various components of following content: CaCl
22H
2o441mg/L, NH
4nO
31600mg/L, KNO
32022mg/L, NaCl907mg/L, MgSO
43361mg/L, NaH
2pO
42H
2o312mg/L, K
2sO
4350mg/L, Na
2sO
4300mg/L, CaCl
2230mg/L, CoCl
26H
2o0.24mg/L, CuSO
45H
2o0.375mg/L, H
3bO
39.3mg/L, MnSO
4h
2o16.9mg/L, Na
2moO
42H
2o0.25mg/L, ZnSO
47H
2o11.5mg/L, KI0.83mg/L, FeSO
47H
2o27.8mg/L, Na
2eDTA2H
2o37.25mg/L, inositol 108mg/L, glycine 3.75mg/L, nicotinic acid 5mg/L, V6(puridoxine hydrochloride) 1.2mg/L, V1(Tyiamine Hd element) 13.5mg/L, vitamin h 0.2mg/L, folic acid 0.88mg/L, vitamin C 1.76mg/L, vitamin b3 1.9mg/L.
Further, above-mentioned EM medium also comprises following component: 6-BA(6-benzyl aminoadenine) 0.2mg/L.
Further, above-mentioned M medium also comprises that auxiliary material is: agar 7g/L, white sugar 30g/L.
The invention has the beneficial effects as follows: compare with existing medium, adopt EM medium of the present invention, the American elm group training seedling mean height rate of increase improves more than 5 times, and average growth coefficient improves more than 2.3 times.Be proven, adopt EM medium of the present invention to solve the needs of the seedling industrialized production of American elm group training, and growing way be vigorous, characters of progenies is stable, reduces production costs, and increases economic efficiency.
Accompanying drawing explanation
Fig. 1 is the sorite differentiation rate comparison diagrams of 4 white elm clones in 5 kinds of medium; As can be seen from Figure 1,4 white elm clones are at A2(EM) sorite differentiation rate in medium is apparently higher than the sorite differentiation rate in A1, A3, A4, A5 medium;
Fig. 2 is the individual plant clear height growth rate comparison diagrams of 4 white elm clones in 5 kinds of medium; As can be seen from Figure 2,4 white elm clones individual plant clear height growth rate in A2 medium is apparently higher than the high growth rate in A1, A3, A4, A5 medium;
Fig. 3 is No. 286 American elm amount of growth contrasts in five kinds of medium of A1-A5;
Fig. 4 is that No. 65225 American elms contrast in amount of growth in five kinds of medium of A1-A5.
Embodiment
For features such as the novelty of abundant performance this invention, practicality, high efficiency, further elaborate the present invention below.Embodiment 1
American elm group training seedling pretreatment: select growth cycle, condition of culture, the consistent American elm group training seedling of health degree, and be that big or small identical simple bud, Multiple Buds are a plurality of standby by the cutting of American elm group training seedling.
The required growing environment of American elm group training seedling is: 25 ℃ of temperature (± 2 ℃), under the gnotobasis of 12 hours light application time every days, shoot proliferation is cultivated 21d.
The present invention be take minimal medium contrast screening as main, and in addition low concentration propagation hormone 6-BA to be to guarantee the normal growth of American elm group training seedling, and agar 7g/L, and white sugar 30g/L is auxiliary material.
In order to contrast existing American elm productive culture base and difference of the present invention, the present invention chooses ms+BA0.2+S3%(A1), EM+6-BA0.2+S3%(A2), dkw+6-BA0.2+S3%(A3), wpm+6-BA0.5+S3%(A4), wpm+6-BA0.2+S3%(A5), 5 kinds of minimal mediums also add hormon and compare analysis, finally determine optimal medium.
Medium of the present invention---EM medium, according to American elm knowhow for many years, take MS, WPM, DKW medium is basis, reintegrates the EM medium that creates the growth of suitable American elm group training seedling, its formula table refers to table 1.
Each nutrient media components of table 1 and content
American elm group training seedling minimal medium comparative trial, each is processed and refers to table 2:
Each processes table 2 American elm group training seedling minimal medium
Remarks: S represents white sugar.
Experiment result:
1. the comparing result of different culture media sorite differentiation rate
Comparative trial by 4 white elm clones on 5 dissimilar expansion breeding culture mediums, show that white elm clones sorite expands the mean value of numerous increment test data, in Table 3.
The sorite differentiation rate of 4 white elm clones of table 3 in 5 kinds of medium
Amount of growth situation statistical value according to 4 white elm clones in table 3 on 5 dissimilar expansion breeding culture mediums, is used the mapping of sigmaplot software, draws 4 white elm clones and carries out same clone sorite differentiation rate block diagram and compare and analyze, and refers to accompanying drawing 1.From chart 3 and Fig. 1, can find out, 4 white elm clones are at A2(EM) sorite differentiation rate in medium is apparently higher than the sorite differentiation rate in A1, A3, A4, A5 medium.
2. the contrast of different culture media individual plant clear height growth rate
Comparative trial by 4 white elm clones on 5 dissimilar expansion breeding culture mediums, draws the mean value of white elm clones individual plant clear height growth rate test data, in Table 4.
The individual plant clear height growth rate of 4 white elm clones of table 4 in 5 kinds of medium
Amount of growth situation statistical value according to 4 white elm clones in table 4 on 5 dissimilar expansion breeding culture mediums, use the mapping of sigmaplot software, draw 4 white elm clones and carry out same clone individual plant clear height growth rate block diagram and compare and analyze, refer to accompanying drawing 2.
From table 4 and Fig. 2, can find out, 4 white elm clones individual plant clear height growth rate in A2 medium is apparently higher than the high growth rate in A1, A3, A4, A5 medium.
The present invention tests growing state comparing result and refers to accompanying drawing 3-Fig. 4.From Fig. 3-Fig. 4, can find out: No. 286 and No. 65225 American elms growing fine A2 medium.
In above-mentioned American elm minimal medium; use the American elm group training seedling amount of growth after EM medium subculture to be all greater than other contrast groups; and grow fine; through too much culture value-added coefficient is comparatively stable; this formula has not only kept the good characteristic of salt tolerant American elm; and reduced production cost, in large-scale production, brought higher economic benefit.
By analysis and the processing of above-mentioned two result of the tests, can obviously find out, American elm minimal medium improves more than 5 times at the group training seedling mean height rate of increase compared with other types medium, and average growth coefficient improves more than 2.3 times.Therefore, take EM medium as basic medium optimum Salt And Alkali Tolerance fast-growing American elm group training seedling growth.
Along with the increase of hormone, reproduction coefficient can be significantly improved within the specific limits.This experiment main purpose, with the basis that is established as of best Salt And Alkali Tolerance fast-growing American elm minimal medium, contrasts the impact of different minimal mediums on Salt And Alkali Tolerance fast-growing American elm growth and reproduction coefficient.In actual production process, prove that the EM medium under higher concentration somatotropin can make the average reproduction coefficient of American elm reach more than 6 times, and the growth of group training seedling is vigorous.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (3)
1. a Salt And Alkali Tolerance fast-growing American elm group training seedling subculture medium, is characterized in that, comprises the various components of following content: CaCl
22H
2o441mg/L, NH
4nO
31600mg/L, KNO
32022mg/L, NaCl907mg/L, MgSO
43361mg/L, NaH
2pO
42H
2o312mg/L, K
2sO
4350mg/L, Na
2sO
4300mg/L, CaCl
2230mg/L, CoCl
26H
2o0.24mg/L, CuSO
45H
2o0.375mg/L, H
3bO
39.3mg/L, MnSO
4h
2o16.9mg/L, Na
2moO
42H
2o0.25mg/L, ZnSO
47H
2o11.5mg/L, KI0.83mg/L, FeSO
47H
2o27.8mg/L, Na
2eDTA2H
2o37.25mg/L, inositol 108mg/L, glycine 3.75mg/L, nicotinic acid 5mg/L, V61.2mg/L, V113.5mg/L, vitamin h 0.2mg/L, folic acid 0.88mg/L, vitamin C 1.76mg/L, vitamin b3 1.9mg/L.
2. a kind of Salt And Alkali Tolerance fast-growing American elm group training seedling subculture medium as claimed in claim 1, is characterized in that, described medium also comprises following component: 6-BA0.2mg/L.
3. a kind of Salt And Alkali Tolerance fast-growing American elm group is trained seedling subculture medium as claimed in claim 1 or 2, it is characterized in that, described medium also comprises that auxiliary material is: agar 7g/L, white sugar 30g/L.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494108A (en) * | 2016-02-29 | 2016-04-20 | 山东省林木种质资源中心 | Tissue culture rapid propagation method of fast-growing Ulmus pumila |
| CN104429958B (en) * | 2014-11-26 | 2016-08-24 | 上海杉一植物科技有限公司 | The method of rejuvenation in American elm tissue cultured seedling bottle |
| CN107079814A (en) * | 2017-04-21 | 2017-08-22 | 山东省林业科学研究院 | A kind of Salt And Alkali Tolerance fast-growing white elm clones Callus of Leaf induction and differentiation method |
| CN108308023A (en) * | 2018-01-15 | 2018-07-24 | 山东省林业科学研究院 | A kind of saline-alkali tolerant fast-growing white elm clones adventitious bud tissue culture rooting method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USPP21608P2 (en) * | 2009-08-03 | 2011-01-04 | Kazimier Kenneth K | Lacebark elm tree named ‘MON1’ |
| CN102726294A (en) * | 2012-06-27 | 2012-10-17 | 上海杉一植物科技有限公司 | In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling |
| CN102934612A (en) * | 2012-11-23 | 2013-02-20 | 上海杉一植物科技有限公司 | Subculture method of salt-tolerance ulmus pumila tissue culture seedlings |
| USPP23756P2 (en) * | 2011-12-14 | 2013-07-23 | Hebei Academy of Forestry Science | Elm tree named ‘Meirenyu’ |
-
2014
- 2014-01-23 CN CN201410031841.9A patent/CN103734018B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USPP21608P2 (en) * | 2009-08-03 | 2011-01-04 | Kazimier Kenneth K | Lacebark elm tree named ‘MON1’ |
| USPP23756P2 (en) * | 2011-12-14 | 2013-07-23 | Hebei Academy of Forestry Science | Elm tree named ‘Meirenyu’ |
| CN102726294A (en) * | 2012-06-27 | 2012-10-17 | 上海杉一植物科技有限公司 | In-bottle rooting method for salt tolerance ulmus pumila tissue culture seedling |
| CN102934612A (en) * | 2012-11-23 | 2013-02-20 | 上海杉一植物科技有限公司 | Subculture method of salt-tolerance ulmus pumila tissue culture seedlings |
Non-Patent Citations (3)
| Title |
|---|
| MUKUND R.SHUKLA: "In vitro conservation of American elm (Ulmus americana):potential role of auxin metabolismin sustained plant proliferation", 《CAN.J.FOR.RES》, vol. 42, 31 December 2012 (2012-12-31), pages 686 - 697 * |
| 王静华: "两种不同种源地白榆的组织培养与叶片再生研究", 《中国农学通报》, vol. 25, no. 5, 31 December 2009 (2009-12-31), pages 110 - 115 * |
| 王静华: "白榆的组织培养与叶片再生研究", 《西北林学院学报》, vol. 24, no. 5, 31 December 2009 (2009-12-31), pages 74 - 77 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104429958B (en) * | 2014-11-26 | 2016-08-24 | 上海杉一植物科技有限公司 | The method of rejuvenation in American elm tissue cultured seedling bottle |
| CN105494108A (en) * | 2016-02-29 | 2016-04-20 | 山东省林木种质资源中心 | Tissue culture rapid propagation method of fast-growing Ulmus pumila |
| CN107079814A (en) * | 2017-04-21 | 2017-08-22 | 山东省林业科学研究院 | A kind of Salt And Alkali Tolerance fast-growing white elm clones Callus of Leaf induction and differentiation method |
| CN108308023A (en) * | 2018-01-15 | 2018-07-24 | 山东省林业科学研究院 | A kind of saline-alkali tolerant fast-growing white elm clones adventitious bud tissue culture rooting method |
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