CN103725746A - Coli group and colibacillus testing medium - Google Patents
Coli group and colibacillus testing medium Download PDFInfo
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- CN103725746A CN103725746A CN201210380855.2A CN201210380855A CN103725746A CN 103725746 A CN103725746 A CN 103725746A CN 201210380855 A CN201210380855 A CN 201210380855A CN 103725746 A CN103725746 A CN 103725746A
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Abstract
The invention discloses a coli group and colibacillus testing medium which is prepared by dissolving 20 grams of peptone, 5 grams of lactose, 2 grams of bile salt, 1.5 grams of monopotassium phosphate, 4 grams of monopotassium phosphate, 5 grams of sodium chloride, 0.1 gram of 5-bromo-4-chloro-3-indole galactoside, 0.1gram of 4- methylumbelliferone glucuronide, 1 gram of aspartic acid, 0.5 gram of casamino acid, 1 gram of yeast extracts and 20 grams of agar in 1000ml of deionized water, and the coli group and colibacillus testing medium is not only used as a plate medium, can also be used as an analoids form, and is low in cost and convenient to popularize and use.
Description
Technical field
The present invention relates to a kind of substratum, what be specifically related to is a kind of coliform and E. coli detection substratum.
Background technology
Coliform is distributed more widely, at warm-blooded animal ight soil and nature, extensively exists.Investigation shows, coliform bacterium is present in warm-blooded animal ight soil, the mankind often movable place and the place that has fecal pollution more, and the people, animal ight soil to external world pollution of environment is the major cause that coliform exists at nature.In ight soil, mainly with typical intestinal bacteria, be main, more with other types of coliform in external environment.Coliform is one of important indicator of evaluating food hygiene quality, be widely used in food sanitation both at home and abroad at present, and intestinal bacteria is evaluated the important indicator of food sanitation especially.Detect at present coliform and colibacillary reagent simultaneously and mainly use the chloro-3-of the bromo-4-of 5-to draw diindyl galactoside (X-Gal) and the chloro-3-of the bromo-4-of 5-draws two kinds of enzyme substratess of diindyl-β-D-Glucose thuja acid (X-Gluc), high cost, is unfavorable for universal use.
Summary of the invention
For addressing the above problem, the present invention proposes a kind of coliform and E. coli detection substratum, the present invention, not only as plate substratum, also can be used as analoids form, and cost is low, is beneficial to universal use.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the present invention is achieved through the following technical solutions:
Coliform and E. coli detection substratum, be dissolved in 1000ml deionized water and formed by peptone 20g, lactose 5g, bile salt 2g, potassium primary phosphate 1.5g, potassium primary phosphate 4g, the chloro-3-indyl of the bromo-4-of sodium-chlor 5g, 5-galactoside 0.1g, 4-methyl fan type ketone glucosiduronate 0.1g, aspartic acid 1g, casamino acids 0.5g, yeast extract 1g and agar 20g.
Further, the chloro-3-indyl of the bromo-4-of described 5-galactoside is beta-galactosidase enzymes substrate.
Further, described 4-methyl fan type ketone glucosiduronate is beta-D-galactosidase substrate.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention, not only as plate culture medium, also can be used as analoids form, and cost is low, is beneficial to universal use.
Embodiment
Coliform and E. coli detection substratum, be dissolved in 1000ml deionized water and formed by peptone 20g, lactose 5g, bile salt 2g, potassium primary phosphate 1.5g, potassium primary phosphate 4g, the chloro-3-indyl of the bromo-4-of sodium-chlor 5g, 5-galactoside 0.1g, 4-methyl fan type ketone glucosiduronate 0.1g, aspartic acid 1g, casamino acids 0.5g, yeast extract 1g and agar 20g.
Further, the chloro-3-indyl of the bromo-4-of described 5-galactoside is beta-galactosidase enzymes substrate.
Further, described 4-methyl fan type ketone glucosiduronate is beta-D-galactosidase substrate.
Principle of the present invention is:
1, by required basic nutrition and coliform selective substances of coliform such as peptone, lactose, bile salts, formed, and add the bromo-4-of beta-galactosidase enzymes substrate 5-chloro-3-indyl galactoside (X-GAL) and make coliform colour developing, add beta-D-galactosidase substrate 4-methyl fan type ketone glucosiduronate (MUG) simultaneously and make intestinal bacteria bacterium colony under uviolizing, show the selective coloration culture medium of blue-fluorescence.
2, specific implementation method: peptone 20g, lactose 5g, bile salt 2g, potassium primary phosphate 1.5g, potassium primary phosphate 4g, the chloro-3-indyl of the bromo-4-of sodium-chlor 5g, 5-galactoside 0.1g, 4-methyl fan type ketone glucosiduronate 0.1g, aspartic acid 1g, casamino acids 0.5g, yeast extract 1g, agar 20g are dissolved in 1000ml deionization, make coliform and intestinal bacteria selective coloration culture medium after autoclave sterilization.
3, get clinical separating Escherichia coli bacterial strain 7 strains, intestinal bacteria reference culture ATCC25922, clinical separated klebsiella 3 strains, enterobacter cloacae 2 strains, streptococcus aureus 2 strains, Streptococcus viridans 3 strains, staphylococcus epidermidis 1 strain, the 2 strain inoculations of scalp staphylococcus, cultivate 24 hours for 37 ℃.Comprise that the colibacillary coliform of 8 strain shows nattierblue bacterium colony, irradiate counterfeit bank note distinction and all show blue-fluorescence with UV-lamp 8 strain intestinal bacteria, Gram-positive bacteria growing is not good, the transparent not colour developing of colony colourless.
4, this substratum, not only as plate substratum, also can be used as analoids form.
Claims (3)
1. coliform and E. coli detection substratum, is characterized in that: by peptone 20g, lactose 5g, bile salt 2g, potassium primary phosphate 1.5g, potassium primary phosphate 4g, the chloro-3-indyl of the bromo-4-of sodium-chlor 5g, 5-galactoside 0.1g, 4-methyl fan type ketone glucosiduronate 0.1g, aspartic acid 1g, casamino acids 0.5g, yeast extract 1g and agar 20g, be dissolved in 1000ml deionized water and formed.
2. coliform according to claim 1 and E. coli detection substratum, is characterized in that: the chloro-3-indyl of the bromo-4-of described 5-galactoside is beta-galactosidase enzymes substrate.
3. coliform according to claim 1 and E. coli detection substratum, is characterized in that: described 4-methyl fan type ketone glucosiduronate is beta-D-galactosidase substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210380855.2A CN103725746A (en) | 2012-10-10 | 2012-10-10 | Coli group and colibacillus testing medium |
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| CN201210380855.2A CN103725746A (en) | 2012-10-10 | 2012-10-10 | Coli group and colibacillus testing medium |
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| CN103725746A true CN103725746A (en) | 2014-04-16 |
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| CN201210380855.2A Pending CN103725746A (en) | 2012-10-10 | 2012-10-10 | Coli group and colibacillus testing medium |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388525A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Chromogenic medium used for detecting escherichia coli O157:H7 |
| CN104388521A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Chromogenic medium used for detecting escherichia coli O157:H7 |
| CN104498391A (en) * | 2014-11-27 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Escherichia coli and culture method of culture medium thereof |
| CN106086159A (en) * | 2016-06-20 | 2016-11-09 | 中国疾病预防控制中心环境与健康相关产品安全所 | A kind of zymolyte culture medium that can simultaneously detect two kinds of fecal pollution indicator bacterias and application thereof |
Citations (2)
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| CN101067600A (en) * | 2007-06-14 | 2007-11-07 | 中国农业科学院饲料研究所 | Aquatic pathogenic bacterium colony induction signaling molecule detecting method and special reagent kit thereof |
| CN102660777A (en) * | 2012-04-28 | 2012-09-12 | 云南省农业科学院生物技术与种质资源研究所 | Method for constructing suppression subtractive hybridization (SSH) library of oryza rufipogon threatened by bacterial blight germs |
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2012
- 2012-10-10 CN CN201210380855.2A patent/CN103725746A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101067600A (en) * | 2007-06-14 | 2007-11-07 | 中国农业科学院饲料研究所 | Aquatic pathogenic bacterium colony induction signaling molecule detecting method and special reagent kit thereof |
| CN102660777A (en) * | 2012-04-28 | 2012-09-12 | 云南省农业科学院生物技术与种质资源研究所 | Method for constructing suppression subtractive hybridization (SSH) library of oryza rufipogon threatened by bacterial blight germs |
Non-Patent Citations (1)
| Title |
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| 甄宏太等: "应用4-甲基伞形酮β-D-葡萄糖苷酸快速检测大肠杆菌", 《现代商检科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388525A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Chromogenic medium used for detecting escherichia coli O157:H7 |
| CN104388521A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Chromogenic medium used for detecting escherichia coli O157:H7 |
| CN104498391A (en) * | 2014-11-27 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Escherichia coli and culture method of culture medium thereof |
| CN106086159A (en) * | 2016-06-20 | 2016-11-09 | 中国疾病预防控制中心环境与健康相关产品安全所 | A kind of zymolyte culture medium that can simultaneously detect two kinds of fecal pollution indicator bacterias and application thereof |
| CN106086159B (en) * | 2016-06-20 | 2019-10-29 | 中国疾病预防控制中心环境与健康相关产品安全所 | A kind of zymolyte culture medium that can detect two kinds of fecal pollution indicator bacterias simultaneously and its application |
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Application publication date: 20140416 |