CN103725745A - Chromogenic counting medium for mycete and microzyme - Google Patents
Chromogenic counting medium for mycete and microzyme Download PDFInfo
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- CN103725745A CN103725745A CN201210380507.5A CN201210380507A CN103725745A CN 103725745 A CN103725745 A CN 103725745A CN 201210380507 A CN201210380507 A CN 201210380507A CN 103725745 A CN103725745 A CN 103725745A
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- chromogenic
- counting
- substratum
- medium
- colour developing
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Abstract
The invention discloses a chromogenic counting medium for mycete and microzyme, and the chromogenic counting medium is prepared as follows: dissolving 20g of peptone, 5g of glucose, 10g of sodium chloride, 0.01g of colistin sulphate, 0.3g of chloramphenicol, 0.01g of tetrazolium violet and 20g of agar in 1000ml deionized water for high temperature and high pressure sterilization to prepare a chromogenic plate medium. The chromogenic counting medium is short in culture time, small in bacterial colony, and beneficial for bacterial colony counting.
Description
Technical field
The present invention relates to a kind of substratum, what be specifically related to is a kind of mould yeast colour developing counting substratum.
Background technology
Yeast and mold is extensively present in air, water and soil, and the overwhelming majority is harmless, but some mould is harmful, and mould also can cause that food goes mouldy, and can stimulate human body alimentary canal, stomach etc., serious also can liver injury, cause food poisoning.Mould yeast is used potato white sugar agar (PDA), sabouraud's agar (Sabouraud's agar) etc., and these culture medium culturing overlong time, bacterium colony are excessive, be unfavorable for bacterium counting number.
Summary of the invention
For addressing the above problem, the present invention proposes a kind of mould yeast colour developing counting substratum, incubation time of the present invention is short, bacterium colony is little, be beneficial to bacterium counting number.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the present invention is achieved through the following technical solutions:
Mould yeast color developing culture medium, by peptone 20g, glucose 5g, sodium-chlor 10g, colistine sulfate 0.01g, paraxin 0.3g, the purple 0.01g of tetrazole and agar 20g, be dissolved in 1000ml deionized water and form, after autoclave sterilization, make colour developing dull and stereotyped.
Further, described bacterium colony colour developing counting substratum is plate substratum or test paper type or detection lug type.
Compared with prior art, the invention has the beneficial effects as follows:
Incubation time of the present invention is short, bacterium colony is little, be beneficial to bacterium counting number, and can be made into the variforms such as test paper type, detection lug type.
Embodiment
Mould yeast color developing culture medium, by peptone 20g, glucose 5g, sodium-chlor 10g, colistine sulfate 0.01g, paraxin 0.3g, the purple 0.01g of tetrazole and agar 20g, be dissolved in 1000ml deionized water and form, after autoclave sterilization, make colour developing plate substratum.
Further, described bacterium colony colour developing counting substratum is plate substratum or test paper type or detection lug type.
Principle of the present invention is:
1, purple containing peptone 10 ~ 20g, glucose 1 ~ 20g, sodium-chlor 5 ~ 10g, colistine sulfate 0.001 ~ 0.1g, paraxin 0.01 ~ 0.5g, TTV(tetrazole in every 1000ml substratum) 0.005 ~ 0.5, agar 15 ~ 20g.
2, specifically implement: get peptone 20g, glucose 5g, sodium-chlor 10g, colistine sulfate 0.01g, paraxin 0.3g, tetrazole purple (TTV) 0.01g, agar 20g, use 1000ml deionized water dissolving, after high-temperature sterilization, be cooled to 50 ~ 60 ℃, water disposable plate, make mould yeast color developing culture medium.
3, get mould 3 strains, yeast 2 strains separated in environment, and the bacterium such as the intestinal bacteria of clinical separation, klebsiella, enterobacter cloacae, streptococcus aureus, scalp staphylococcus, Streptococcus viridans mix after inoculation, at 25 ℃, cultivate 24 ~ 36 hours, mould yeast shows red-purple, and bacterium has no growth.
4, the invention is not restricted to agar plate substratum, can be made into the variforms such as test paper type, detection lug type.
Claims (2)
1. substratum is counted in the colour developing of mould yeast, it is characterized in that: by peptone 20g, glucose 5g, sodium-chlor 10g, colistine sulfate 0.01g, paraxin 0.3g, the purple 0.01g of tetrazole and agar 20g, be dissolved in 1000ml deionized water and form, after autoclave sterilization, make colour developing plate substratum.
2. mould yeast colour developing counting substratum according to claim 1, is characterized in that: described bacterium colony color developing culture medium is plate substratum or test paper type or detection lug type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210380507.5A CN103725745A (en) | 2012-10-10 | 2012-10-10 | Chromogenic counting medium for mycete and microzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210380507.5A CN103725745A (en) | 2012-10-10 | 2012-10-10 | Chromogenic counting medium for mycete and microzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103725745A true CN103725745A (en) | 2014-04-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210380507.5A Pending CN103725745A (en) | 2012-10-10 | 2012-10-10 | Chromogenic counting medium for mycete and microzyme |
Country Status (1)
| Country | Link |
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| CN (1) | CN103725745A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110117791A (en) * | 2019-05-23 | 2019-08-13 | 电子科技大学 | A kind of binding force promotor for holes on high density interconnected printed circuit board brownification liquid |
| CN110129807A (en) * | 2019-05-23 | 2019-08-16 | 电子科技大学 | It is a kind of for copper/iron-based material restrainer and pickling solution |
| CN112481350A (en) * | 2020-11-18 | 2021-03-12 | 中科健康产业集团股份有限公司 | Composite culture medium for rapidly detecting total number of moulds and yeasts and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900293A (en) * | 2006-07-11 | 2007-01-24 | 中国医学科学院皮肤病研究所 | Modified skin tinea fungus test culture medium |
| CN101177668A (en) * | 2007-12-05 | 2008-05-14 | 四川省医学科学院（四川省人民医院） | Novel neisseria gonorrhoeae culture medium and method for making same |
-
2012
- 2012-10-10 CN CN201210380507.5A patent/CN103725745A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1900293A (en) * | 2006-07-11 | 2007-01-24 | 中国医学科学院皮肤病研究所 | Modified skin tinea fungus test culture medium |
| CN101177668A (en) * | 2007-12-05 | 2008-05-14 | 四川省医学科学院（四川省人民医院） | Novel neisseria gonorrhoeae culture medium and method for making same |
Non-Patent Citations (4)
| Title |
|---|
| YURIKO NAGANO等: "Development of selective media for the isolation of yeasts and filamentous fungi from the sputum of adult patients with cystic fibrosis (CF)", 《JOURNAL OF CYSTIC FIBROSIS》 * |
| 卫生部合理用药专家委员会: "《临床微生物与感染》", 31 July 2010, 中国医药科技出版社 * |
| 徐薇等: "足癣致病菌的菌种分析", 《中国医刊》 * |
| 朱红梅等: "医学真菌常用培养基的制备和应用", 《中国真菌学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110117791A (en) * | 2019-05-23 | 2019-08-13 | 电子科技大学 | A kind of binding force promotor for holes on high density interconnected printed circuit board brownification liquid |
| CN110129807A (en) * | 2019-05-23 | 2019-08-16 | 电子科技大学 | It is a kind of for copper/iron-based material restrainer and pickling solution |
| CN110117791B (en) * | 2019-05-23 | 2023-11-10 | 电子科技大学 | Binding force promoter for brown oxide liquid of high-density interconnection printed circuit board |
| CN112481350A (en) * | 2020-11-18 | 2021-03-12 | 中科健康产业集团股份有限公司 | Composite culture medium for rapidly detecting total number of moulds and yeasts and preparation method thereof |
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Application publication date: 20140416 |