CN103706336B - A kind of preparation method of the adsorbent for absorption degradation water pollutant - Google Patents
A kind of preparation method of the adsorbent for absorption degradation water pollutant Download PDFInfo
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Abstract
The present invention relates to a kind of preparation method of the adsorbent for absorption degradation seawater pollution thing, comprise the following steps: (1) activation biology cellulose fermenting and producing bacterial strain, preparation fermenting and producing seed liquor; (2) seed liquor adds in fermentation medium by the inoculum concentration of 8-12% by volume, also has access to oil degradation bacteria in described fermentation medium; (3) standing for fermentation 8-20 days at 25-35 DEG C; (4) collect the biological cellulose membrane that media surface produces, alkali cleaning, is dried to water content lower than 50%.According to the adsorbent that the method prepares, by fixing for oil degradation bacteria by the 3-D solid structure of biology cellulose wherein formed membrane structure, directly can be layed in water surface, increase the contact area of adsorbent and water surface; Simultaneously biology cellulose stable in properties, oil degradation bacteria wherein can slowly efficiently discharge, and absorbs the oil pollution in degraded water body, has immobilization, the feature such as efficient, permanently effective.
Description
Technical field
The present invention relates to water pollutant biodegrading process, especially relate to a kind of preparation method of the adsorbent for absorption degradation water pollutant.
Background technology
In recent years, along with the demand that countries in the world day by day increase oil and goods thereof, exploit, load and unload, transport and utilize oil spill accident in oil process to happen occasionally at sea, a large amount of crude oil is not only caused to run off, also severe contamination is caused to environment, threaten the ecological environment of water body and land-based area with being on the rise, have long-term harm to water body environment, natural resources and culture resources etc., therefore, water body oil spill become people must faced by great environmental problem.
The method of current process water body oil spill mainly contains Physical, chemical method and bioanalysis.Chemical method is the technology path of comparative maturity in point-source pollution is administered, and has obtained propagation and employment widely.But adopt chemical method in water body oil spill is administered, need to add chemical agent in water body, the chemical agent wherein added may produce secondary pollution on the one hand, can produce short-term or long-term impact on the other hand on water ecological setting.The features such as bioremediation technology has efficiently, economy, safety, non-secondary pollution, become the on-the-spot important selection approach removing water body oil spill, particularly to the oil sheet that mechanical device cannot be removed, when simultaneously restriction uses chemical agent again, use and biological prostheticly show larger superiority.Physical absorption is that a kind for the treatment of effeciency is high, cost is low, without sediment pollution, simple to operate and can not cause the method for secondary pollution and destruction to water ecological setting, can solve the problem existing for chemical method preferably.But the cycle of bioremediation technology is longer, be subject to the restriction that whether there is highly effective petroleum surfactant hydrocarbon degradation bacteria, physisorphtion faces the saturated rear problem needing to remarket adsorbent of absorption simultaneously.
Biology cellulose (Biocellulose, BC) refers at different conditions, by acetic acid Pseudomonas (
acetobacter), Agrobacterium (
agrobacterium), rhizobium (
rhizobium) and Sarcina (
sarcina) etc. in the cellulosic general designation of certain Microbe synthesis.Compared with native cellulose, it has the advantageous properties such as high-purity, high-crystallinity, high polymerization degree, high retentiveness.Be widely used in every field.
Summary of the invention
Technical problem solved by the invention is to provide a kind of preparation method of the adsorbent for absorption degradation seawater pollution thing, according to the adsorbent that the method prepares, by the 3-D solid structure of biology cellulose, oil degradation bacteria is fixed wherein, form membrane structure, directly can be layed in water surface, increase the contact area of adsorbent and water surface; Simultaneously biology cellulose stable in properties, oil degradation bacteria wherein can slowly efficiently discharge, and absorbs the oil pollution in degraded water body, has immobilization, the feature such as efficient, permanently effective.
The technical solution used in the present invention is:
For a preparation method for the adsorbent of absorption degradation seawater pollution thing, comprise the following steps:
(1) biology cellulose fermenting and producing bacterial strain is activated, preparation fermenting and producing seed liquor;
(2) seed liquor adds in fermentation medium by the inoculum concentration of 8-12% by volume, also has access to oil degradation bacteria in described fermentation medium;
(3) standing for fermentation 8-20 days at 25-35 DEG C;
(4) collect the biological cellulose membrane that media surface produces, alkali cleaning, is dried to water content lower than 50%.
In said method, the oil degradation bacteria in described step (2) is the Mixed Microbes of bacterium and fungi, and wherein bacterium comprises pseudomonad, corynebacteria, methane pole hair bacillus, bites oil arthrobacterium and Nocard's bacillus; Fungi comprises burr sliding rust umbrella (Hebelomamesophaeusm), kakgeusia breast mushroom (Lactariusinsulsns), pinecone bolete (Strobilomycesfloccopus), cortina bacterium (Cortinariusrussus), thorn spore little gram of Mildy Way bacterium, Phanerochaete chrysosporium.The ratio of the inoculum concentration of described bacterium and fungi is (1-8): (1-6), and the effective bacterium number in inoculation post-fermentation and culture base is 10
6-10
10cfu/mL.Described bacterium is non-carbohydrate zymophyte, mainly hydro carbons zymophyte, and it can decompose utilization
hydrocarbonclass, as methane, ethane, aliphatic hydrocarbon, aromatic hydrocarbon etc., effectively can decompose the oil pollution in water body; And combine the root system of plant fungi of taking from soil, effectively can improve the decomposition utilizing status to this compound of oil.Namely these mushrooms are added in the fermentation medium of biology cellulose at earlier fermentation by the present invention, because it does not utilize carbohydrate, can not have an impact to the fermentation of biology cellulose fermentation bacterial strain.Then it is in the fermentation production process of biology cellulose; effectively be wrapped in three space networks of biology cellulose equably; not only serve protective effect, effectively can also reduce after it is directly released to water body in large and cannot fix with current are arbitrarily floating the defect effectively worked.
In said method, the biology cellulose fermenting and producing bacterial strain in described step (1) is acetobacter xylinum or gluconate pyracetobacillus.
In said method, the preparation method of seed liquor is in described step (1): be seeded to by activated strains in seed culture medium, 25-35 DEG C, 150-220rpm cultivates 20-48h; Containing the enough Carbon and nitrogen sources for strain growth in described seed culture medium.Carbon source is preferably sweet mellow wine, sorbierite and/or sucrose.
In said method, the carbon source of described fermentation medium is glucose, fructose, sucrose and/or coconut water.
In said method, in described step (2), in fermentation medium, be also preferably added with emulsifying agent, preferred nonionic surface active agent, as APES, high-carbon fatty alcohol polyoxyethylene ether or polyether nonionic surfactant etc.Its effect is when the biological cellulose membrane finally obtained being used in water surface, biological cellulose membrane can slow imbibition, wherein fixing emulsifying agent can oil in emulsification water body, is absorbed by biological cellulose membrane, thus decompose by the oil degradation bacteria wherein fixed utilization.
The adsorbent that preparation method of the present invention prepares, has the proterties of desciccator diaphragm, has the feature of easy transport, storage; It can also have different size dimension and thickness simultaneously, can determine its size and thickness by the degree of water pollution.The time of film is produced in the fermentation of suitable adjustment biology cellulose, obtains the biological cellulose membrane of different size size and thickness.When it is applied to water body oil spill, arbitrarily can not flow with ocean current, have certain fixation, simultaneously it floats over water surface, effectively contacts when can realize being formed with the oil pollution of water surface maximum area long.Wherein fixing oil degradation bacteria can effectively decompose the oil utilized in water body, reduces water pollution.Biological cellulose membrane after using by adding the form of appropriate cellulase by its biological decomposition in water body, can also not remain.The adsorbent that preparation method of the present invention prepares has immobilization, the feature such as efficient, permanently effective.
Detailed description of the invention
Below in conjunction with detailed description of the invention and contrast experiment, the present invention is further illustrated, but specific implementation method is not hereinafter appreciated that limitation of the present invention.The various change that those of ordinary skill in the art can make apparently on basis of the present invention and change, should all within the scope of the present invention.
Embodiment 1:
For a preparation method for the adsorbent of absorption degradation seawater pollution thing, comprise the following steps:
(1) biology cellulose fermenting and producing bacterial strain gluconate pyracetobacillus is activated, preparation fermenting and producing seed liquor: seed fluid nutrient medium is containing sweet mellow wine 25g/l, yeast extract 5g/l, peptone 3g/l, fill 500ml fluid nutrient medium in every 1 liter of shaking flask, 180 turns, the 30 degree lower 24h that cultivate obtain seed liquor;
(2) seed liquor adds in fermentation medium by the inoculum concentration of 10% by volume, and described fermentation medium contains: acetic acid: 0.2%, sucrose: 10%, and all the other are coconut water; In fermentation medium, accessing oil degradation bacteria again, is pseudomonad 2, the Mixed Microbes of biting oil arthrobacterium 2 and Phanerochaete chrysosporium 3, and the effective bacterium number in inoculation post-fermentation and culture base is 10
8cfu/mL;
(3) 30 DEG C, quiescent culture 14 days in 250ml culture plate;
(4) mycoderm that media surface is formed is collected, at room temperature rinse twice with 0.5NNaOH, extremely neutral by rinsed with deionized water again, it is 30% or following that recompression is dried to water content, forms adsorbent of the present invention: the biology cellulose film containing oil degradation bacteria.
Embodiment 2:
For a preparation method for the adsorbent of absorption degradation seawater pollution thing, comprise the following steps:
(1) biology cellulose fermenting and producing bacterial strain gluconate pyracetobacillus is activated, preparation fermenting and producing seed liquor: seed fluid nutrient medium is containing sweet mellow wine 25g/l, yeast extract 5g/l, peptone 3g/l, fill 500ml fluid nutrient medium in every 1 liter of shaking flask, 180 turns, the 30 degree lower 24h that cultivate obtain seed liquor;
(2) seed liquor adds in fermentation medium by the inoculum concentration of 8% by volume, and described fermentation medium is containing acetic acid: 0.2%, glucose: 10%, and all the other are coconut water; In fermentation medium, access oil degradation bacteria again, be the Mixed Microbes of corynebacteria 1, methane pole hair bacillus 3, burr sliding rust umbrella 1, pinecone bolete 1, cortina bacterium 1, the effective bacterium number in inoculation post-fermentation and culture base is 10
6cfu/mL; The OPEO of 0.01% is added again in fermentation medium;
(3) 30 DEG C, quiescent culture 14 days in 250ml culture plate;
(4) mycoderm that media surface is formed is collected, at room temperature rinse twice with 0.5NNaOH, extremely neutral by rinsed with deionized water again, it is 50% or following that recompression is dried to water content, forms adsorbent of the present invention: the biology cellulose film containing oil degradation bacteria.
Embodiment 3:
For a preparation method for the adsorbent of absorption degradation seawater pollution thing, comprise the following steps:
(1) biology cellulose fermenting and producing bacterial strain gluconate pyracetobacillus is activated, preparation fermenting and producing seed liquor: seed fluid nutrient medium is containing sweet mellow wine 25g/l, yeast extract 5g/l, peptone 3g/l, fill 500ml fluid nutrient medium in every 1 liter of shaking flask, 180 turns, the 30 degree lower 24h that cultivate obtain seed liquor;
(2) seed liquor adds in fermentation medium by the inoculum concentration of 12% by volume, and described fermentation medium is containing acetic acid: 0.2%, sucrose: 5%, fructose: 5%, and all the other are coconut water; Oil degradation bacteria is accessed again in fermentation medium, for pseudomonad 1, corynebacteria 1, methane pole hair bacillus 1, bite the Mixed Microbes that oil arthrobacterium 1, Nocard's bacillus 1 and burr slide rust umbrella 1, kakgeusia breast mushroom 1, pinecone bolete 1, cortina bacterium 1, thorn spore little gram of Mildy Way bacterium 1, Phanerochaete chrysosporium 1, the effective bacterium number in inoculation post-fermentation and culture base is 10
10cfu/mL; The polyether nonionic surfactant (ethylene oxide adduct of polypropylene glycol) of 0.01% is added again in fermentation medium;
(3) 30 DEG C, quiescent culture 14 days in 250ml culture plate;
(4) mycoderm that media surface is formed is collected, at room temperature rinse twice with 0.5NNaOH, extremely neutral by rinsed with deionized water again, it is 10% or following that recompression is dried to water content, forms adsorbent of the present invention: the biology cellulose film containing oil degradation bacteria.
Experimental example:
In laboratory conditions, first the water body of preparation simulation water body oil spill: get river or seawater, add 0.2%(v/v) oil, then the biology cellulose film prepared by embodiment 1-3 is directly layed in water surface, process 15-30 days, the oil substance detected afterwards in water body remains.Result shows, oil substance degradation rate reaches more than 70%, and such as Cr, Cu, Mn and Zn content of some heavy metal element in water body also reduces 68%, 72%, 56% and 46%.Illustrate that adsorbent of the present invention effectively acts on when having long to water body oil spill.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that the common engineers and technicians in this area make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.
Claims (9)
1. for a preparation method for the adsorbent of absorption degradation water pollutant, it is characterized in that: comprise the following steps:
(1) biology cellulose fermenting and producing bacterial strain is activated, preparation fermenting and producing seed liquor;
(2) seed liquor adds in fermentation medium by the inoculum concentration of 8-12% by volume, also has access to oil degradation bacteria, and be added with emulsifying agent in described fermentation medium;
(3) standing for fermentation 8-20 days at 25-35 DEG C;
(4) collect the biological cellulose membrane that media surface produces, alkali cleaning, is dried to water content lower than 50%.
2. preparation method according to claim 1, it is characterized in that: the oil degradation bacteria in described step (2) is the Mixed Microbes of bacterium and fungi, wherein bacterium comprises pseudomonad, corynebacteria, methane pole hair bacillus, bites oil arthrobacterium and Nocard's bacillus; Fungi comprises burr sliding rust umbrella, kakgeusia breast mushroom, pinecone bolete, cortina bacterium, thorn spore little gram of Mildy Way bacterium, Phanerochaete chrysosporium.
3. preparation method according to claim 2, is characterized in that: the ratio of the inoculum concentration of wherein said bacterium and fungi is (1-8): (1-6), and the effective bacterium number in inoculation post-fermentation and culture base is 10
6-10
10cfu/mL.
4. preparation method according to claim 1, is characterized in that: the biology cellulose fermenting and producing bacterial strain in described step (1) is acetobacter xylinum or gluconate pyracetobacillus.
5. preparation method according to claim 1, is characterized in that: the preparation method of seed liquor is in described step (1): be seeded to by activated strains in seed culture medium, 25-35 DEG C, 150-220rpm cultivates 20-48h; Containing the enough Carbon and nitrogen sources for strain growth in described seed culture medium.
6. preparation method according to claim 5, is characterized in that: described carbon source is sweet mellow wine, sorbierite and/or sucrose.
7. preparation method according to claim 1, is characterized in that: the carbon source of described fermentation medium is glucose, fructose, sucrose and/or coconut water.
8. preparation method according to claim 1, is characterized in that: described emulsifying agent is APES, high-carbon fatty alcohol polyoxyethylene ether or polyether nonionic surfactant.
9. the adsorbent that the method described in any one of claim 1-8 prepares.
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| CN101134955A (en) * | 2007-08-02 | 2008-03-05 | 中国石油化工股份有限公司 | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same |
| CN101973640A (en) * | 2010-09-21 | 2011-02-16 | 东北电力大学 | Method for treating malachite green dye waste water |
| CN102604924A (en) * | 2012-03-02 | 2012-07-25 | 中国人民解放军海军医学研究所 | Offshore oil degrading microbial inoculum and preparation method thereof |
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| US6573087B2 (en) * | 1999-07-06 | 2003-06-03 | William F. Lehr | Packaged timed release microorganisms and absorbent matrix for degradation of hydrocarbons |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101134955A (en) * | 2007-08-02 | 2008-03-05 | 中国石油化工股份有限公司 | Solid composite micro-organism micro-balloon for organic contaminant biodegradation and method for preparing the same |
| CN101973640A (en) * | 2010-09-21 | 2011-02-16 | 东北电力大学 | Method for treating malachite green dye waste water |
| CN102604924A (en) * | 2012-03-02 | 2012-07-25 | 中国人民解放军海军医学研究所 | Offshore oil degrading microbial inoculum and preparation method thereof |
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