CN101973640A - Method for treating malachite green dye waste water - Google Patents
Method for treating malachite green dye waste water Download PDFInfo
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- CN101973640A CN101973640A CN 201010290607 CN201010290607A CN101973640A CN 101973640 A CN101973640 A CN 101973640A CN 201010290607 CN201010290607 CN 201010290607 CN 201010290607 A CN201010290607 A CN 201010290607A CN 101973640 A CN101973640 A CN 101973640A
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Abstract
The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.
Description
Technical field
The invention belongs to using microbe and field of environment engineering technology, relate to the method for utilizing a kind of novel fixation support treatment based on immobilized microorganisms waste water from dyestuff.Relate in particular to the modified bacteria cellulose film that utilizes immobilized white rot fungus and handle the malachite green waste water from dyestuff.
Background technology
Malachite green is a kind of deleterious triphenylmethane chemicals, and by phenyl aldehyde and N, accelerine behind the recessive alkali body of condensation generation tetramethyl-for the diamino tritane, is made by the secret oxidation of plumbic oxide in acid medium in hydrochloric acid or sulfuric acid.At present, photocatalytic oxidation, microwave method, iron oxygen cell method and diatomite porous china-clay method etc. are mainly adopted in the processing of malachite green waste water from dyestuff, but these methods exist that cost height, cycle are long, operational stability is poor, cause shortcoming such as secondary pollution easily.
Occurring in nature whiterot fungi (Phanerochaete chrysosporium) is a kind of important Biological resources, the bigger organic pollutant of polynuclear aromatics class and the toxicity of can degrading.Whiterot fungi can produce peroxidase such as lignin peroxidase LiP and manganese peroxidase MnP etc. outside the born of the same parents in handling the dyestuff process, these enzymes have properties such as substrate is non-specific, and can cause a series of free radical reaction, thereby to dyestuff decolour, degraded and mineralising.
Though whiterot fungi has plurality of advantages, it also exists processing efficiency low in suspension culture degraded system, enzyme system poor stability, problem such as resistance, toxin immunity, anti-high load capacity are poor, solid-liquid separation difficulty.
The method that immobilized white rot fungus is commonly used has entrapping method, absorption method etc., and fixation support commonly used has wood chip, polyurethane foam, gac, polyvinyl alcohol, alginate calcium.But there are a lot of problems in above-mentioned carrier, as preparation process is complicated, immobilization efficiency is low, operational stability is poor, easily environment is caused secondary pollution after discarded.
Summary of the invention
Purpose of the present invention: carry out modification by the bacteria cellulose film that acetobacter xylinum is produced and handle, make it become the excellent carrier of immobilized microorganism, and then being used for fixing whiterot fungi, set up a kind of simple, effective means of handling the malachite green waste water from dyestuff, the immobilized whiterot fungi of the bacteria cellulose film that is modified activity in to malachite green dye wastewater treatment process is more stable, immunity from interference is stronger, and carrier is lasting, durable, can reuse repeatedly.
A kind of treatment process of malachite green waste water from dyestuff, form by following process and step:
(1) preparation of bacteria cellulose film
With being kept at strain number on the inclined-plane is that the acetobacter xylinum Gluconacetobacterxylinum of CGMCC NO:1.1812 is linked in the 50mL seed culture medium, and the per-cent that each amounts of components accounts for seed culture medium quality consumption in the described seed culture medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; Cultivated 2 days with 160rpm on the gas bath constant temperature shaking table, the acetobacter xylinum nutrient solution that obtains activating, get this acetobacter xylinum nutrient solution 10mL and be linked in the 125mL fermention medium, the per-cent that each amounts of components accounts for fermention medium quality consumption in the described fermention medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O 0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; 30 ℃ leave standstill cultivation 7 days, generate the pale yellow glue plasma membrane at liquid level, and film is taken out, and wash repeatedly with distilled water, remove striping surface medium and impurity; Film is soaked in the NaOH solution of 0.1mol/L, 80 ℃ are boiled 60min again, remove the thalline and the residual substratum of mycoderm inside, wash repeatedly to neutrality with distilled water then, obtain bacteria cellulose film;
Above-mentioned acetobacter xylinum is available from Chinese common micro-organisms culture presevation administrative center, and strain number is CGMCC NO:1.1812;
(2) modification of bacteria cellulose film is handled
The bacteria cellulose film that 40～60g step (1) is obtained joins in the 1mol/L sodium hydroxide solution of 100～180mL, adds 20～40mL epoxy chloropropane again, behind 60 ℃ of water bath with thermostatic control reaction 30～60min, takes out the film washing with acetone; After the washing, add the quadrol of 5.5～9mL and the distilled water of 49.5～81mL again; Behind the shake well, in 80 ℃ of waters bath with thermostatic control, heat 30～60min, take out film then and be washed till neutrality with distilled water, after using the salt acid elution 3 times of 1% weight percentage again, distilled water is washed till neutrality, again after the acetone rinse, vacuum-drying is to constant weight, obtains the bacteria cellulose film after the modification;
(3) the modified bacteria cellulose film is the immobilization of the whiterot fungi of CGMCC NO:5.776 to strain number
PH=7.0, concentration that the modified bacteria cellulose film that 6～9g step (2) is obtained joins 80～105mL are in the phosphoric acid buffer of 0.2mol/L, add the glutaraldehyde water solution of 6～7mL, 25% weight percentage again; In 40 ℃ of following gentle agitation reaction 30～60min, take out film and use distilled water and 0.2mol/L successively, the phosphoric acid buffer washing of pH=7.0, vacuum-drying then; Dried bacteria cellulose film joins in the restricted substratum of whiterot fungi liquid of 110～130mL, and each amounts of components is respectively in the restricted substratum of described whiterot fungi liquid: KH
2PO
42g/L; MgSO
47H
2O 0.25g/L; MnSO
40.5mg/L; Anhydrous FeSO
40.1g/L; NaCl 1.0mg/L; NH
4NO
32g/L; CaCl
20.1g/L; Glucose 15g/L; VB
15.0mg/L; PH=6.0～7.0; Add 12～15mL whiterot fungi spore liquid after the sterilization again; In 28 ℃, cultivated 48 hours on the 120rpm shaking table, to wash repeatedly three times with sterilized water behind the taking-up film, this moment, the film surface formed the very thin white mycoderm of one deck, the modified bacteria cellulose film of being fixed whiterot fungi;
Above-mentioned whiterot fungi is available from Chinese common micro-organisms culture presevation administrative center, and strain number is CGMCC NO:5.776;
(4) the immobilization bacterial strain is numbered the modified bacteria cellulose film of whiterot fungi of CGMCC NO:5.776 to the processing of malachite green waste water from dyestuff
The modified bacteria cellulose film of the immobilized white rot fungus that 1～2g step (3) is obtained, join in the 50mL malachite green waste water from dyestuff, the preparation process of above-mentioned malachite green waste water from dyestuff is as follows: take by weighing 20～40mg malachite green, be dissolved in the restricted substratum of the described whiterot fungi liquid of 1L above-mentioned steps (3), obtain the malachite green waste water from dyestuff; Under 28 ℃, on gas bath constant temperature shaking table, handled 5 days, measured the colourity of waste water from dyestuff every 24 hours, after 5 days with 120rpm, discard the waste water from dyestuff after the processing, keep immobilized bacterium, add the waste water from dyestuff of the same concentrations of the new preparation of 50mL again, treatment condition are the same, measured the colourity of waste water from dyestuff every 24 hours, with 5 days be a circulation, so repeat 5 times, the clearance of waste water from dyestuff colourity maintains more than 86% all the time.
The present invention compared with prior art exists obvious improvement and positive effect:
1. the present invention uses new thinking and the approach that provide for bacteria cellulose film.
2. the bacteria cellulose film toughness after the modification improves, and corrosion resistance strengthens, and has reduced the interference of carrier to whiterot fungi processing malachite green waste water from dyestuff effect itself.
3. the method for the modified bacteria cellulose film of immobilized white rot fungus processing malachite green waste water from dyestuff has operability, and simple to operate, and treatment effect obviously reaches good stability, and is affected by environment little.
4. the modified bacteria cellulose film can be degraded by environmental microorganism after discarded, and the fertilizer that becomes plant-growth can not cause secondary pollution.
Embodiment
Embodiment 1
The preparation of whiterot fungi spore liquid:
From preserving a small amount of thalline of picking on the inclined-plane of whiterot fungi that strain number is CGMCC NO:5.776, method of scoring is inoculated on the solid medium, and the per-cent that each amounts of components accounts for solid medium quality consumption in the described solid medium is respectively: potato dextrose agar 3.8%; KH
2PO
40.3%; MgSO
47H
2O cultivates after 5 days for 0.15%, 30 ℃, and whiterot fungi spore mycelia is increased in a large number, in plate, inject a small amount of sterilized water, scrape spore powder with the push rod method, through 4 layers of lens wiping paper elimination mycelium, the oyster white spore suspension that obtains is the whiterot fungi spore liquid, and 4 ℃ of refrigerators are preserved standby;
Whiterot fungi is available from Chinese common micro-organisms culture presevation administrative center in the above-mentioned steps, and strain number is CGMCCNO:5.776.
Embodiment 2
The preparation of bacteria cellulose film:
With being kept at strain number on the inclined-plane is that the acetobacter xylinum Gluconacetobacterxylinum of CGMCC NO:1.1812 is linked in the 50mL seed culture medium, and the per-cent that each amounts of components accounts for seed culture medium quality consumption in the described seed culture medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; Cultivated 2 days with 160rpm on the gas bath constant temperature shaking table, the acetobacter xylinum nutrient solution that obtains activating, get this acetobacter xylinum nutrient solution 10mL and be linked in the 125mL fermention medium, the per-cent that each amounts of components accounts for fermention medium quality consumption in the described fermention medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; 30 ℃ leave standstill cultivation 7 days, generate the pale yellow glue plasma membrane at liquid level, and film is taken out, and wash repeatedly with distilled water, remove striping surface medium and impurity; Film is soaked in the NaOH solution of 0.1mol/L, 80 ℃ are boiled 60min again, remove the thalline and the residual substratum of mycoderm inside, wash repeatedly to neutrality with distilled water then, obtain bacteria cellulose film.
Acetobacter xylinum is available from Chinese common micro-organisms culture presevation administrative center in the above-mentioned steps, and strain number is CGMCCNO:1.1812;
Embodiment 3:
The modification of bacteria cellulose film is handled:
Get the 1mol/L sodium hydroxide solution that bacteria cellulose film 40g puts into 100mL, add the epoxy chloropropane of 20mL again, behind 60 ℃ of water bath with thermostatic control reaction 30min, take out the film washing with acetone, to remove small organic molecule residual on the film; After the washing, add quadrol and the 49.5mL distilled water of 5.5mL again, behind the shake well, in 80 ℃ of waters bath with thermostatic control, heat 30min, take out film then and be washed till neutrality with distilled water, use the salt acid elution 3 times of 1% weight percentage again after, distilled water is washed till neutrality, again after the acetone rinse, vacuum-drying is to constant weight, this moment film the snappiness grow, it is faint yellow that the surface is, smooth, glossy, this is the bacteria cellulose film after the modification;
The modified bacteria cellulose film is the immobilization of the whiterot fungi of CGMCC NO:5.776 to strain number:
Get the modified bacteria cellulose film that the 6g above-mentioned steps obtains and be put into phosphoric acid buffer (the Phosphate Buffer that contains 80mL, 0.2mol/L, pH=7.0) in the Erlenmeyer flask, the glutaraldehyde water solution that adds 6mL 25% weight percentage again, 40 ℃ of gentle agitation reaction 30min take out film and use distilled water and phosphoric acid buffer (Phosphate Buffer, 0.2mol/L successively, pH=7.0) washing, vacuum-drying then; Bacteria cellulose film after the above-mentioned glutaraldehyde processing is put in the Erlenmeyer flask that contains the restricted substratum of 110mL whiterot fungi liquid, and each amounts of components is respectively in the restricted substratum of described whiterot fungi liquid: KH
2PO
42g/L; MgSO
47H
2O 0.25g/L; MnSO
40.5mg/L; Anhydrous FeSO
40.1g/L; NaCl 1.0mg/L; NH
4NO
32g/L; CaCl
20.1g/L; Glucose 15g/L; VB
15.0mg/L; PH=6.0～7.0 (the restricted substratum of whiterot fungi liquid is formed identical in following examples); Add the whiterot fungi spore liquid that 12mL embodiment 1 obtains after the sterilization again, in 28 ℃, cultivated 48 hours on the 120rpm shaking table, wash repeatedly three times with sterilized water after taking out film, this moment, the bacteria cellulose film surface formed the very thin white mycoderm of one deck, was the modified bacteria cellulose film of immobilized white rot fungus.
Embodiment 4:
The modification of bacteria cellulose film is handled:
Get the 1mol/L sodium hydroxide solution that bacteria cellulose film 50g puts into 150mL, add the epoxy chloropropane of 30mL again, behind 60 ℃ of water bath with thermostatic control reaction 45min, take out the film washing with acetone, to remove small organic molecule residual on the film; After the washing, add quadrol and the 76.5mL distilled water of 8.5mL again, behind the shake well, in 80 ℃ of waters bath with thermostatic control, heat 45min, take out film then and be washed till neutrality with distilled water, use 1% weight percentage salt acid elution 3 times again after, distilled water is washed till neutrality, again after the acetone rinse, vacuum-drying is to constant weight, this moment film the snappiness grow, it is faint yellow that the surface is, smooth, glossy, this is the bacteria cellulose film after the modification;
The modified bacteria cellulose film is the immobilization of the whiterot fungi of CGMCC NO:5.776 to strain number:
Get the modified bacteria cellulose film that the 7g above-mentioned steps obtains and be put into phosphoric acid buffer (the Phosphate Buffer that contains 90mL, 0.2mol/L, pH=7.0) in the Erlenmeyer flask, the glutaraldehyde water solution that adds 6.5mL 25% weight percentage again, 40 ℃ of gentle agitation reaction 45min take out film and use distilled water and phosphate buffer solution (Phosphate Buffer, 0.2mol/L successively, pH=7.0) washing, vacuum-drying then; Bacteria cellulose film after the above-mentioned glutaraldehyde processing is put in the Erlenmeyer flask that contains the restricted substratum of 120mL whiterot fungi liquid; Add the whiterot fungi spore liquid that 13mL embodiment 1 obtains after the sterilization again, in 28 ℃, cultivated 48 hours on the 120rpm shaking table, wash repeatedly three times with sterilized water after taking out film, this moment, the bacteria cellulose film surface formed the very thin white mycoderm of one deck, was the modified bacteria cellulose film of immobilized white rot fungus.
Embodiment 5:
The modification of bacteria cellulose film is handled:
Get the 1mol/L sodium hydroxide solution that bacteria cellulose film 60g puts into 180mL, add the epoxy chloropropane of 40mL again, behind 60 ℃ of water bath with thermostatic control reaction 60min, take out the film washing with acetone, to remove small organic molecule residual on the film; After the washing, add quadrol and the 81mL distilled water of 9mL again, behind the shake well, in 80 ℃ of waters bath with thermostatic control, heat 60min, take out film then and be washed till neutrality with distilled water, use the salt acid elution 3 times of 1% weight percentage again after, distilled water is washed till neutrality, again after the acetone rinse, vacuum-drying is to constant weight, this moment film the snappiness grow, it is faint yellow that the surface is, smooth, glossy, this is the bacteria cellulose film after the modification;
The modified bacteria cellulose film is the immobilization of the whiterot fungi of CGMCC NO:5.776 to strain number:
Get the modified bacteria cellulose film that the 9g above-mentioned steps obtains and be put into phosphoric acid buffer (the Phosphate Buffer that contains 105mL, 0.2mol/L, pH=7.0) in the Erlenmeyer flask, the glutaraldehyde water solution that adds 7mL 25% weight percentage again, 40 ℃ of gentle agitation reaction 60min take out film and use distilled water and phosphoric acid buffer (Phosphate Buffer, 0.2mol/L successively, pH=7.0) washing, vacuum-drying then; Bacteria cellulose film after the above-mentioned glutaraldehyde processing is put in the Erlenmeyer flask that contains the restricted substratum of 130mL whiterot fungi liquid; Add the whiterot fungi spore liquid that 15mL embodiment 1 obtains after the sterilization again, in 28 ℃, cultivated 48 hours on the 120rpm shaking table, wash repeatedly three times with sterilized water after taking out film, this moment, the bacteria cellulose film surface formed the very thin white mycoderm of one deck, was the modified bacteria cellulose film of immobilized white rot fungus.
Embodiment 6:
The immobilization bacterial strain is numbered the modified bacteria cellulose film of whiterot fungi of CGMCC NO:5.776 to the processing of malachite green waste water from dyestuff:
Get the modified bacteria cellulose film that 1g immobilization bacterial strain is numbered the whiterot fungi of CGMCC NO:5.776, and the same polyvinyl alcohol sodium alginate of changing whiterot fungi surely of equal in quality, join respectively in the Erlenmeyer flask that contains 50mL malachite green waste water from dyestuff, the preparation process of above-mentioned malachite green waste water from dyestuff is as follows: take by weighing the 20mg malachite green, be dissolved in the restricted substratum of 1L whiterot fungi liquid, promptly obtain the malachite green waste water from dyestuff; Under 28 ℃, on gas bath constant temperature shaking table, handled 5 days with the 120rpm shaking table, measured the colourity of waste water from dyestuff every 24 hours, after 5 days, discard the waste water from dyestuff after the processing, keep immobilized bacterium, the 20mg/L malachite green waste water from dyestuff that in this Erlenmeyer flask, adds the new preparation of 50mL again, treatment condition are the same, measured the colourity of waste water from dyestuff every 24 hours, with 5 days be a circulation, so repeat 5 times, promptly continue with the modified bacteria cellulose symphysis with 1 batch of immobilized white rot fungus and handle 6 batches of waste water from dyestuff, chromaticity removing effect is as shown in table 1:
The different carriers of table 1 immobilized white rot fungus is to the treatment effect of 20mg/L malachite green waste water from dyestuff
As can be seen from Table 1, the modified bacteria cellulose film of 1g immobilized white rot fungus obviously is better than the polyvinyl alcohol-sodium alginate of the immobilized white rot fungus of equal in quality to the treatment effect of 20mg/L malachite green waste water from dyestuff, and the interference that the modified bacteria cellulose film is handled malachite green waste water from dyestuff effect to whiterot fungi is significantly less than polyvinyl alcohol-sodium alginate.
Embodiment 7:
The immobilization bacterial strain is numbered the modified bacteria cellulose film of whiterot fungi of CGMCC NO:5.776 to the processing of malachite green waste water from dyestuff:
Get the modified bacteria cellulose film that 2g immobilization bacterial strain is numbered the whiterot fungi of CGMCC NO:5.776, and the polyvinyl alcohol-sodium alginate of the immobilized white rot fungus of equal in quality, join respectively in the Erlenmeyer flask that contains 50mL malachite green waste water from dyestuff, the preparation process of above-mentioned malachite green waste water from dyestuff is as follows: take by weighing the 40mg malachite green, be dissolved in the restricted substratum of 1L whiterot fungi liquid, promptly obtain the malachite green waste water from dyestuff; Under 28 ℃, on gas bath constant temperature shaking table, handled 5 days with the 120rpm shaking table, measured the colourity of waste water from dyestuff every 24 hours, after 5 days, discard the waste water from dyestuff after the processing, keep immobilized bacterium, the 40mg/L malachite green waste water from dyestuff that in this Erlenmeyer flask, adds the new preparation of 50mL again, treatment condition are the same, measured the colourity of waste water from dyestuff every 24 hours, with 5 days be a circulation, so repeat 5 times, promptly continue with the modified bacteria cellulose symphysis with 1 batch of immobilized white rot fungus and handle 6 batches of waste water from dyestuff, chromaticity removing effect is as shown in table 1:
The different carriers of table 2 immobilized white rot fungus is to the treatment effect of 40mg/L malachite green waste water from dyestuff
As can be seen from Table 2, the modified bacteria cellulose film of 2g immobilized white rot fungus obviously is better than the polyvinyl alcohol-sodium alginate of the immobilized white rot fungus of equal in quality to the treatment effect of 40mg/L malachite green waste water from dyestuff, and the interference that the modified bacteria cellulose film is handled malachite green waste water from dyestuff effect to whiterot fungi is significantly less than polyvinyl alcohol-sodium alginate.
Embodiment 8:
The immobilization bacterial strain is numbered the modified bacteria cellulose film of whiterot fungi of CGMCC NO:5.776 to the processing of malachite green waste water from dyestuff:
Get the modified bacteria cellulose film that 1.5g immobilization bacterial strain is numbered the whiterot fungi of CGMCC NO:5.776, and the polyvinyl alcohol-sodium alginate of the immobilized white rot fungus of equal in quality, join respectively in the Erlenmeyer flask that contains 50mL malachite green waste water from dyestuff, the preparation process of above-mentioned malachite green waste water from dyestuff is as follows: take by weighing the 30mg malachite green, be dissolved in the restricted substratum of 1L whiterot fungi liquid, promptly obtain the malachite green waste water from dyestuff; Under 28 ℃, on gas bath constant temperature shaking table, handled 5 days with the 120rpm shaking table, measured the colourity of waste water from dyestuff every 24 hours, after 5 days, discard the waste water from dyestuff after the processing, keep immobilized bacterium, the 30mg/L malachite green waste water from dyestuff that in this Erlenmeyer flask, adds the new preparation of 50mL again, treatment condition are the same, measured the colourity of waste water from dyestuff every 24 hours, with 5 days be a circulation, so repeat 5 times, promptly continue with the modified bacteria cellulose symphysis with 1 batch of immobilized white rot fungus and handle 6 batches of waste water from dyestuff, chromaticity removing effect is as shown in table 1:
The different carriers of table 3 immobilized white rot fungus is to the treatment effect of 30mg/L malachite green waste water from dyestuff
As can be seen from Table 3,1.5g the modified bacteria cellulose film of immobilized white rot fungus obviously is better than the polyvinyl alcohol-sodium alginate of the immobilized white rot fungus of equal in quality to the treatment effect of 30mg/L malachite green waste water from dyestuff, and the interference that the modified bacteria cellulose film is handled malachite green waste water from dyestuff effect to whiterot fungi is significantly less than polyvinyl alcohol-sodium alginate.
Annotate:
Polyvinyl alcohol-sodium alginate to the immobilization step of whiterot fungi is among the foregoing description 6～embodiment 8:
1. 10g polyvinyl alcohol and 0.5g sodium alginate are joined in the 100mL distilled water, make it dissolving, be cooled to room temperature, and then 0.1g gac input is wherein made it to mix with the boiling water bath heating; In the aseptic technique platform, in above-mentioned gained mixed solution, add the whiterot fungi spore liquid that 10mL embodiment 1 obtains, mix fully;
2. dispose saturated boric acid solution 100ml, regulate pH value to 6.7 with sodium carbonate solution then, in this mixed solution, add 2g calcium chloride, thorough mixing again;
3. will be 1. the gained mixture squeeze into syringe, splash in the mixture 2., splash into that control glue drips size about 2～3mm in the process.Then glue is dripped to be placed in 4 ℃ of refrigerators and preserve 24h, make its gelation, extremely neutral with distilled water flushing at last, promptly polyvinyl alcohol-the sodium alginate of being fixed whiterot fungi places 4 ℃ of refrigerators stand-by, time spent exhaustion moisture.
Claims (1)
1. the treatment process of a malachite green waste water from dyestuff, form by following process and step:
(1) preparation of bacteria cellulose film
With being kept at strain number on the inclined-plane is that the acetobacter xylinum Gluconacetobactcrxylinum of CGMCC NO:1.1812 is linked in the 50mL seed culture medium, and the per-cent that each amounts of components accounts for seed culture medium quality consumption in the described seed culture medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; Cultivated 2 days with 160rpm on the gas bath constant temperature shaking table, the acetobacter xylinum nutrient solution that obtains activating, get this acetobacter xylinum nutrient solution 10mL and be linked in the 125mL fermention medium, the per-cent that each amounts of components accounts for fermention medium quality consumption in the described fermention medium is respectively: glucose 2%; Peptone 0.5%; Yeast extract paste 0.3%; Na
2HPO
412H
2O 0.2%; KH
2PO
40.1%; MgSO
47H
2O 0.025%; Citric acid 0.2%; PH5.8; 30 ℃ leave standstill cultivation 7 days, generate the pale yellow glue plasma membrane at liquid level, and film is taken out, and wash repeatedly with distilled water, remove striping surface medium and impurity; Film is soaked in the NaOH solution of 0.1mol/L, 80 ℃ are boiled 60min again, remove the thalline and the residual substratum of mycoderm inside, wash repeatedly to neutrality with distilled water then, obtain bacteria cellulose film;
(2) modification of bacteria cellulose film is handled
The bacteria cellulose film that 40～60g step (1) is obtained joins in the 1mol/L sodium hydroxide solution of 100～180mL, adds 20～40mL epoxy chloropropane again, behind 60 ℃ of water bath with thermostatic control reaction 30～60min, takes out the film washing with acetone; After the washing, add the quadrol of 5.5～9mL and the distilled water of 49.5～81mL again; Behind the shake well, in 80 ℃ of waters bath with thermostatic control, heat 30～60min, take out film then and be washed till neutrality with distilled water, after using the salt acid elution 3 times of 1% weight percentage again, distilled water is washed till neutrality, again after the acetone rinse, vacuum-drying is to constant weight, obtains the bacteria cellulose film after the modification;
(3) the modified bacteria cellulose film is the immobilization of the whiterot fungi of CGMCC NO:5.776 to strain number
PH=7.0, concentration that the modified bacteria cellulose film that 6～9g step (2) is obtained joins 80～105mL are in the phosphoric acid buffer of 0.2mol/L, add the glutaraldehyde water solution of 6～7mL, 25% weight percentage again; In 40 ℃ of following gentle agitation reaction 30～60min, take out film and use distilled water and 0.2mol/L successively, the phosphoric acid buffer washing of pH=7.0, vacuum-drying then; Dried bacteria cellulose film joins in the restricted substratum of whiterot fungi liquid of 110～130mL, and each amounts of components is respectively in the restricted substratum of described whiterot fungi liquid: KH
2PO
42g/L; MgSO
47H
2O 0.25g/L; MnSO
40.5mg/L; Anhydrous FeSO
40.1g/L; NaCl1.0mg/L; NH
4NO
32g/L; CaCl
20.1g/L; Glucose 15g/L; VB
15.0mg/L; PH=6.0～7.0; Add 12～15mL whiterot fungi spore liquid after the sterilization again; In 28 ℃, cultivated 48 hours on the 120rpm shaking table, to wash repeatedly three times with sterilized water behind the taking-up film, this moment, the film surface formed the very thin white mycoderm of one deck, the modified bacteria cellulose film of being fixed whiterot fungi;
(4) the immobilization bacterial strain is numbered the modified bacteria cellulose film of whiterot fungi of CGMCC NO:5.776 to the processing of malachite green waste water from dyestuff
The modified bacteria cellulose film of the immobilized white rot fungus that 1～2g step (3) is obtained, join in the 50mL malachite green waste water from dyestuff, the preparation process of above-mentioned malachite green waste water from dyestuff is as follows: take by weighing 20～40mg malachite green, be dissolved in the restricted substratum of the described whiterot fungi liquid of 1L above-mentioned steps (3), obtain the malachite green waste water from dyestuff; Under 28 ℃, on gas bath constant temperature shaking table, handled 5 days, measured the colourity of waste water from dyestuff every 24 hours, after 5 days with 120rpm, discard the waste water from dyestuff after the processing, keep immobilized bacterium, add the waste water from dyestuff of the same concentrations of the new preparation of 50mL again, treatment condition are the same, measured the colourity of waste water from dyestuff every 24 hours, with 5 days be a circulation, so repeat 5 times, the clearance of waste water from dyestuff colourity maintains more than 86% all the time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010290607XA CN101973640B (en) | 2010-09-21 | 2010-09-21 | Method for treating malachite green dye waste water |
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| CN102659283A (en) * | 2012-04-28 | 2012-09-12 | 浙江博世华环保科技有限公司 | Process for treating and recovering dye industrial wastewater |
| CN103112955A (en) * | 2013-03-11 | 2013-05-22 | 浙江省农业科学院 | Method for degrading and decolorizing malachite green wastewater by utilizing deinococcus radiodurans R1 |
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| CN103466806A (en) * | 2013-09-09 | 2013-12-25 | 江苏大学 | Process technology for degrading gallic acid in waste water by employing aspergillus oryzae |
| CN103496775A (en) * | 2013-10-18 | 2014-01-08 | 长安大学 | Method for removing malachite green |
| CN103663606A (en) * | 2013-12-09 | 2014-03-26 | 北京工业大学 | Method used for removing malachite green from waste water with sunflower seed shell biomass carbon adsorbent |
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| CN102659283B (en) * | 2012-04-28 | 2013-06-19 | 浙江博世华环保科技有限公司 | Process for treating and recovering dye industrial wastewater |
| CN102659283A (en) * | 2012-04-28 | 2012-09-12 | 浙江博世华环保科技有限公司 | Process for treating and recovering dye industrial wastewater |
| CN103903863A (en) * | 2012-12-27 | 2014-07-02 | 中国科学院上海硅酸盐研究所 | Method for preparing dye-sensitized solar cell by rapidly adsorbing dyes |
| CN103112955A (en) * | 2013-03-11 | 2013-05-22 | 浙江省农业科学院 | Method for degrading and decolorizing malachite green wastewater by utilizing deinococcus radiodurans R1 |
| CN103304041A (en) * | 2013-05-23 | 2013-09-18 | 东北电力大学 | Treatment method of Congo red dye wastewater |
| CN103466806A (en) * | 2013-09-09 | 2013-12-25 | 江苏大学 | Process technology for degrading gallic acid in waste water by employing aspergillus oryzae |
| CN103466806B (en) * | 2013-09-09 | 2016-04-06 | 江苏大学 | Gallic acid Technology in a kind of aspergillus oryzae degrading waste water |
| CN103496775B (en) * | 2013-10-18 | 2015-04-22 | 长安大学 | Method for removing malachite green |
| CN103496775A (en) * | 2013-10-18 | 2014-01-08 | 长安大学 | Method for removing malachite green |
| CN103663606B (en) * | 2013-12-09 | 2015-08-26 | 北京工业大学 | A kind of method utilizing sunflower seed shell biomass charcoal sorbent material to remove waste water Malachite Green |
| CN103663606A (en) * | 2013-12-09 | 2014-03-26 | 北京工业大学 | Method used for removing malachite green from waste water with sunflower seed shell biomass carbon adsorbent |
| CN103706336A (en) * | 2013-12-18 | 2014-04-09 | 海南椰国热带水果食品加工有限公司 | Preparation method of absorbent for absorbing and degrading water pollutants |
| CN103706336B (en) * | 2013-12-18 | 2016-03-30 | 海南椰国热带水果食品加工有限公司 | A kind of preparation method of the adsorbent for absorption degradation water pollutant |
| CN104558212B (en) * | 2015-01-29 | 2017-02-22 | 海南光宇生物科技有限公司 | Application of aminated bio-cellulose to preparation of moist dressing |
| CN104558212A (en) * | 2015-01-29 | 2015-04-29 | 海南光宇生物科技有限公司 | Preparation method of aminated bio-cellulose |
| CN105417726A (en) * | 2015-12-15 | 2016-03-23 | 安徽皖维高新材料股份有限公司 | Method for treating polyvinyl alcohol production wastewater through white rot fungi |
| CN105417726B (en) * | 2015-12-15 | 2018-03-30 | 安徽皖维高新材料股份有限公司 | A kind of method using white-rot fungi processing polyvinyl alcohol production waste water |
| CN106591276A (en) * | 2016-12-23 | 2017-04-26 | 南京理工大学 | Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium |
| CN107096506A (en) * | 2017-04-06 | 2017-08-29 | 浙江理工大学 | A kind of nanometer Fe3O4The preparation method of/etherificate bacteria cellulose Compound Heavy Metals sorbing material |
| CN108408920A (en) * | 2018-03-15 | 2018-08-17 | 湖南大学 | A method of utilizing Penicillium notatum composite degradation malachite green wastewater |
| CN108408920B (en) * | 2018-03-15 | 2021-06-29 | 湖南大学 | Method for degrading malachite green wastewater by using penicillium composite material |
| CN110152621A (en) * | 2019-06-06 | 2019-08-23 | 安徽科技学院 | A kind of Enteromorpha that improves is to the new method of waste water dyestuff malachite green adsorption efficiency |
| CN115385458A (en) * | 2022-09-20 | 2022-11-25 | 常州大学 | Method for degrading organic matters in wastewater by ultrasonic electrochemical coupling white rot fungi |
| CN115385458B (en) * | 2022-09-20 | 2024-04-05 | 常州大学 | Method for degrading organic matters in wastewater by coupling ultrasonic electrochemistry with white rot fungi |
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