CN103705543A - Manure-borne compound probiotics soluble powder for animals and preparation method thereof - Google Patents
Manure-borne compound probiotics soluble powder for animals and preparation method thereof Download PDFInfo
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- CN103705543A CN103705543A CN201310733372.0A CN201310733372A CN103705543A CN 103705543 A CN103705543 A CN 103705543A CN 201310733372 A CN201310733372 A CN 201310733372A CN 103705543 A CN103705543 A CN 103705543A
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Abstract
The invention provides manure-borne compound probiotics soluble powder for animals and a preparation method thereof. The preparation comprises the following components in percentage by weight: 10-45 percent of bacterial strain, 3-10 percent of allicin, 3-7 percent of whey protein, 0.5-3 percent of available energy, 0.2-3 percent of bovine coloctrum, 0.3-1 percent of vitamins, 1-5 percent of cryoprotectant and 26-82 percent of excipient. The preparation method has the characteristics of simplicity, feasibility and suitableness for large-scale industrial production. The multi-bacterial strain soluble powder is simple and feasible in preparation method and convenient to use, has an effect of regulating multiple colonies in animal intestines, can be transported, preserved and used under normal temperature conditions and has wide application and popularization prospects.
Description
Technical field
The present invention relates to a kind of veterinary drug preparation and preparation method thereof, especially a kind of excrement source compound recipe probiotic bacteria soluble powder for animal and its preparation method and application.
Background technology
Animal intestinal health care has become the common recognition of current cultivation industry, only have intestinal is regulated to health care, can improve the alimentation of animal intestinal, improve the resistance of animal to epidemic disease, therefore antibacterials in feedstuff, have substantially all been added, for guaranteeing intestinal health.But, under present food security pressure, antibiotics in the occupation rate of animal cultivation industry by more and more atrophy, as Korea S, European Union, the U.S., Japan etc. add antibiotic by completely forbidding gradually in feedstuff, or in food animal feedstuff, add treatment antibiotics, product requirement to China's export cultivation group or company will be more and more stricter, this present situation is seriously restricting the sustainable development of moving guarantor's industry and cultivation industry, the control of animal intestinal disease is also become to current important topic simultaneously, caused extensive concern.
Microbial ecological agent is one of selection of substitute antibiotics class medicine, and a large amount of evidence probiotic bacterias have obvious health-care effect to the adjusting of animal intestinal normal flora, is keeping being subject to approval aspect animal intestinal health, raising animal disease resistant power.The main bacterial strain of microbial ecological agent is lactic acid bacteria class at present, its resistance is slightly poor, can only under cryogenic conditions, keep active, therefore in production, preservation, transportation and use procedure, there will be degradation phenomenon under Microflora minimizing, bacterial strain vigor, cause it cannot normally bring into play effect.In addition, because thalline proportion is larger, in water, after a period of time, can there is sedimentation, cause animal when drinking-water, cannot guarantee to take in the thalline of sufficient amount.
Microbial population of animal intestinal tract is comparatively complicated, comprises Bifidobacterium, Lactobacillus, Streptococcus, bacillus, enterococcus and other Pseudomonas, and single lactic acid bacteria class product regulates health care comparatively single to intestinal, is unfavorable for regulating other Pseudomonas.At present, on market, microbial ecological agent product mainly contains bacterium liquid, the microcapsule class of cryopreservation, and is the preparation of single bacterial strain, not yet has the compound microbial ecological agent of many bacterial strains, also not yet has soluble powder preparation.
Summary of the invention
To be solved by this invention for a kind of animal excrement source compound recipe probiotic bacteria soluble powder, according to weight percent meter, described preparation comprises following component:
Preferably, according to weight percent meter, described preparation comprises following component:
Preferably, described bacterial strain is selected from enterococcus faecalis, enterococcus faecalis, lactoenterococcus, Lactobacillus plantarum, Lactobacillus bulgaricus, bacillus acidophilus, lactobacillus casei cheese subspecies, Lactobacillus fermenti, Lactobacillus salivarius, lactobacillus lactis, Lactobacillus buchneri, lactobacillus cellobiosas, lactobacillus thermophilus, lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, animal bifidobacteria, Bacillus licheniformis, bacillus subtilis, pediococcus acidilactici, Rhodopseudomonas palustris, produce at least five kinds in propionibacterium acide-propionici, wherein, all bacterial strains are all in separated reversal Eriocheir sinensis monkey feces,
The described quick-acting energy adopts at least one in glyceryl monostearate, triglycerin fat acid esters, cithrol and sucrose fatty acid ester;
Described vitamin is selected from least two kinds in vitamin A, vitamin B1, vitamin B2, pellagra preventive factor, adenine phosphate, vitamin B5, vitamin B6, biotin, FA, vitamin B12, inositol, vitamin C, vitamin D, vitamin E;
Described cryoprotective agent is selected from least one in glycerol, defatted milk powder, trehalose, polyvinylpyrrolidone, mannitol, gelatin, carbomer, polymine, dibastic sodium phosphate, potassium dihydrogen phosphate, sorbitol, glycine, arginine, glutamic acid, thiourea, sodium thiosulfate, the tert-butyl alcohol;
Described excipient is selected from least one in anhydrous glucose, sucrose, soluble starch, oligomeric galactose, oligomeric xylose, oligofructose, starch.
The present invention also provides the method for a kind of animal with excrement source compound recipe probiotic bacteria soluble powder, comprises following step:
Steps A: described bacterial strain, in fluid medium, is cultivated and obtained bacterium liquid, and reconcentration filters, and obtains bacteria suspension;
Step B: by described excrement source probiotic bacteria soluble powder key component, add cryoprotective agent, vitamin, lactalbumin and cattle colostrums in bacteria suspension, obtain bacterium liquid mixture;
Step C: mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
Step D: by intermediate products and described garlicin, the quick-acting energy, excipient, mix subpackage and get final product in three-dimensional motion mixer.
Preferably, in described steps A: the incubation step of bacterium liquid is:
(1) described bacterial strain is inoculated into respectively in corresponding fluid medium and is cultivated, obtain seed liquor;
(2) seed liquor obtaining is fermented in one grade fermemtation culture medium, cultivate and obtain primary seed solution;
(3) primary seed solution obtaining is fermented in second order fermentation culture medium, cultivate and obtain bacterium liquid.
Preferably, the temperature of step (1), (2), (3) is 36 ℃ ± 2 ℃, and rotating speed is 100-140r/min.
Preferably, the incubation time of step (2), (3) is 12-14 hour.
Preferably, the incubation time of step (1) is 20-28 hour.
Preferably, in described steps A, the temperature of thickening filtration is-25 ℃-4 ℃.
Preferably, before deviating from treatment step, described step C must keep ambient temperature at 10-15 ℃.
Preferably, in step B and step D, mixing time is 10-30 minute.
The present invention has following beneficial effect:
1, excrement source probiotic bacteria soluble powder water soluble for animal of the present invention, for the administration of drinking water, has feature easy to use;
2, the present invention uses cryoprotective agent and cattle colostrums, lactalbumin, and thalline is protected; Add vitamin, be conducive to thalline antioxidation;
3, all bacterial strains of the present invention, all in separated reversal Eriocheir sinensis monkey feces, can be preserved, transport and use under normal temperature condition, effectively guarantee thalline vigor and quantity;
4, animal of the present invention is wider by excrement source probiotic bacteria soluble powder bacterium spectrum, and the various floras of animal intestinal are all had to regulating action;
5, preparation method of the present invention also has feature simple, that be suitable for large-scale industrial production.Many bacterial strains soluble powder preparation method is simple, easy to use, and the multiple flora of animal intestinal is all had to regulating action, can at normal temperature condition, transport, preserve and use, and has wide application prospect.
The specific embodiment
Below preferably embodiment of the present invention is described in further detail:
Embodiment 1
Excrement source compound recipe probiotic bacteria soluble powder for a kind of animal, by weight percentage, comprise: enterococcus faecalis 7%, enterococcus faecalis 3%, Lactobacillus bulgaricus 1%, lactobacillus cellobiosas 1%, animal bifidobacteria 3%, garlicin 5%, lactalbumin 4%, the quick-acting energy 1%, cattle colostrums 0.5%, vitamin A 0.3%, vitamin C 0.2%, glycerol 2%, sucrose 72%.
(1) described bacterial strain (enterococcus faecalis, enterococcus faecalis, Lactobacillus bulgaricus, lactobacillus cellobiosas, animal bifidobacteria) is inoculated into respectively in corresponding fluid medium, under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 20 hours, obtains seed liquor;
(2) seed liquor of inoculation 15% quality is fermented in one grade fermemtation culture medium, and under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 12 hours, obtains primary seed solution;
(3) primary seed solution of inoculation 10% quality is fermented in second order fermentation culture medium, and under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 12 hours, obtains bacterium liquid;
(4) bacterium liquid soft thickening filtration under-20 ℃ of cryogenic conditions, obtains bacteria suspension;
(5) by described many bacterial strains soluble powder key component, add glycerol, vitamin A, vitamin C, lactalbumin and cattle colostrums in bacteria suspension, continuous stirring 10 minutes, obtains bacterium liquid mixture;
(6) mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
(7) by intermediate products and described garlicin, triglycerin fat acid esters, anhydrous glucose, in three-dimensional motion mixer, mix 10 minutes continuously.Subpackage and get final product.
Embodiment 2
Excrement source compound recipe probiotic bacteria soluble powder for a kind of animal, by weight percentage, comprise: animal bifidobacteria 5%, enterococcus faecalis 10%, pediococcus acidilactici 1%, bacillus subtilis 1%, Lactobacillus salivarius 3%, garlicin 6%, lactalbumin 5%, the quick-acting energy 2%, cattle colostrums 0.8%, vitamin A 0.1%, vitamin C 0.6%, glycerol 3%, sucrose 62.5%.
(1) described bacterial strain (animal bifidobacteria, enterococcus faecalis, pediococcus acidilactici, bacillus subtilis, Lactobacillus salivarius) is inoculated into respectively in corresponding fluid medium, under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 28 hours, obtains seed liquor;
(2) seed liquor of inoculation 15% quality is fermented in one grade fermemtation culture medium, and under 36 ℃ ± 2 ℃, 140r/min condition, continuous culture is 14 hours, obtains primary seed solution;
(3) primary seed solution of inoculation 10% quality is fermented in second order fermentation culture medium, and under 36 ℃ ± 2 ℃, 140r/min condition, continuous culture is 14 hours, obtains bacterium liquid;
(4) bacterium liquid soft thickening filtration under-10 ℃ of cryogenic conditions, obtains bacteria suspension;
(5) by described many bacterial strains soluble powder key component, add glycerol, vitamin A, vitamin C, lactalbumin and cattle colostrums in bacteria suspension, continuous stirring 20 minutes, obtains bacterium liquid mixture;
(6) mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
(7), by intermediate products and described garlicin, glyceryl monostearate, sucrose, in three-dimensional motion mixer, mix 30 minutes continuously.Subpackage and get final product.
Embodiment 3
Excrement source compound recipe probiotic bacteria soluble powder for a kind of animal, by weight percentage, comprise: enterococcus faecalis 15%, lactobacillus thermophilus 3%, bifidobacterium bifidum 3%, lactobacillus rhamnosus 5%, product propionibacterium acide-propionici 1%, garlicin 6%, lactalbumin 5%, the quick-acting energy 3%, cattle colostrums 2%, vitaminB10 .1%, biotin 0.9%, thiourea 1%, the tert-butyl alcohol 4%, soluble starch 51%.
(1) described bacterial strain (enterococcus faecalis, lactobacillus thermophilus, bifidobacterium bifidum, lactobacillus rhamnosus, product propionibacterium acide-propionici) is inoculated into respectively in corresponding fluid medium, under 36 ℃ ± 2 ℃, 130r/min condition, continuous culture is 24 hours, obtains seed liquor;
(2) seed liquor of inoculation 15% quality is fermented in one grade fermemtation culture medium, and under 36 ℃ ± 2 ℃, 130r/min condition, continuous culture is 13 hours, obtains primary seed solution;
(3) primary seed solution of inoculation 10% quality is fermented in second order fermentation culture medium, and under 36 ℃ ± 2 ℃, 130r/min condition, continuous culture is 13 hours, obtains bacterium liquid;
(4) bacterium liquid soft thickening filtration under-5 ℃ of cryogenic conditions, obtains bacteria suspension;
(5) by described many bacterial strains soluble powder key component, add glycerol, vitamin A, vitamin C, lactalbumin and cattle colostrums in bacteria suspension, continuous stirring 20 minutes, obtains bacterium liquid mixture;
(6) mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
(7), by intermediate products and described garlicin, cithrol, soluble starch, in three-dimensional motion mixer, mix 20 minutes continuously.Subpackage and get final product.
Embodiment 4
Excrement source compound recipe probiotic bacteria soluble powder for a kind of animal, by weight percentage, comprise: enterococcus faecalis 5%, enterococcus faecalis 2%, Lactobacillus bulgaricus 2%, lactobacillus thermophilus 3%, bifidobacterium bifidum 3%, lactobacillus rhamnosus 5%, product propionibacterium acide-propionici 1%, garlicin 5%, lactalbumin 7%, the quick-acting energy 2%, cattle colostrums 1%, vitamin PP 0.2%, inositol 0.9%, glutamic acid 0.5%, the tert-butyl alcohol 4%, oligomeric galactose 58.4%.
(1) described bacterial strain (enterococcus faecalis, enterococcus faecalis, Lactobacillus bulgaricus, lactobacillus thermophilus, bifidobacterium bifidum, lactobacillus rhamnosus, product propionibacterium acide-propionici) is inoculated into respectively in corresponding fluid medium, under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 24 hours, obtains seed liquor;
(2) seed liquor of inoculation 15% quality is fermented in one grade fermemtation culture medium, and under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 13 hours, obtains primary seed solution;
(3) primary seed solution of inoculation 10% quality is fermented in second order fermentation culture medium, and under 36 ℃ ± 2 ℃, 120r/min condition, continuous culture is 13 hours, obtains bacterium liquid;
(4) bacterium liquid soft thickening filtration under 0 ℃ of cryogenic conditions, obtains bacteria suspension;
(5) by described many bacterial strains soluble powder key component, add glycerol, vitamin A, vitamin C, lactalbumin and cattle colostrums in bacteria suspension, continuous stirring 20 minutes, obtains bacterium liquid mixture;
(6) mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
(7), by intermediate products and described garlicin, sucrose fatty acid ester, oligomeric galactose, in three-dimensional motion mixer, mix 20 minutes continuously.Subpackage and get final product.
Embodiment 5
Excrement source compound recipe probiotic bacteria soluble powder for a kind of animal, by weight percentage, comprise: bacillus acidophilus 1%, enterococcus faecalis 3%, Lactobacillus buchneri 1%, bifidobacterium longum 0.5%, bifidobacterium adolescentis 3%, Bacillus licheniformis 5%, Rhodopseudomonas palustris 0.1%, garlicin 8%, lactalbumin 7%, the quick-acting energy 2%, cattle colostrums 1%, vitamin B6 0.2%, vitamin B12 0.9%, arginine 0.5%, glycine 4%, oligofructose 62.8%.
(1) described bacterial strain (bacillus acidophilus, enterococcus faecalis, Lactobacillus buchneri, bifidobacterium longum, bifidobacterium adolescentis, Bacillus licheniformis, Rhodopseudomonas palustris) is inoculated into respectively in corresponding fluid medium, under 36 ℃ ± 2 ℃, 110r/min condition, continuous culture is 24 hours, obtains seed liquor;
(2) seed liquor of inoculation 15% quality is fermented in one grade fermemtation culture medium, and under 36 ℃ ± 2 ℃, 110r/min condition, continuous culture is 13 hours, obtains primary seed solution;
(3) primary seed solution of inoculation 10% quality is fermented in second order fermentation culture medium, and under 36 ℃ ± 2 ℃, 110r/min condition, continuous culture is 13 hours, obtains bacterium liquid;
(4) bacterium liquid soft thickening filtration under 4 ℃ of cryogenic conditions, obtains bacteria suspension;
(5) by described many bacterial strains soluble powder key component, add glycerol, vitamin A, vitamin C, lactalbumin and cattle colostrums in bacteria suspension, continuous stirring 20 minutes, obtains bacterium liquid mixture;
(6) mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
(7), by intermediate products and described garlicin, sucrose fatty acid ester, oligofructose, in three-dimensional motion mixer, mix 20 minutes continuously.Subpackage and get final product.
Embodiment 6: excrement source compound recipe probiotic bacteria soluble powder in-vitro evaluation result for animal
According to version < < Chinese veterinary pharmacopoeia > > dissolution method the 3rd subtraction unit in 2010, (embodiment 1 to take appropriate embodiment sample, embodiment 3, embodiment 5), take simulated gastric fluid and simulated intestinal fluid as dissolution medium, rotating speed is 100r/min, operation in accordance with the law, respectively 0, after 2 hours, get dissolution fluid 5ml, filtering with microporous membrane with diameter 0.22 μ m, get subsequent filtrate, standby, according to certain extension rate, on plating medium, cultivate, calculate probiotic bacteria bacterium colony sum, and with market on the probiotic products of buying compare (control drug), result is as shown in table 1: average total plate count after compound recipe probiotic bacteria soluble powder acts in simulated gastric fluid and simulated intestinal fluid.
Table 1
(unit: CFU/g)
| Sample | Simulated gastric fluid 30 minutes | Simulated gastric fluid 2h | Simulated intestinal fluid 30 minutes | Simulated intestinal fluid 2h |
| Embodiment 1 | 7.51×10 8 | 7.35×10 8 | 1.25×10 9 | 5.85×10 8 |
| Embodiment 3 | 7.44×10 8 | 7.16×10 8 | 1.12×10 9 | 6.55×10 8 |
| Embodiment 5 | 7.06×10 8 | 7.59×10 8 | 1.36×10 9 | 5.98×10 8 |
| Control drug | ﹤10 4 | ﹤10 3 | 8.06×10 8 | 6.33×10 7 |
Result shows, compound recipe probiotic bacteria soluble powder of the present invention continuous action 2 hours in simulated gastric fluid and simulated intestinal fluid, and its average total plate count is all significantly better than matched group, shows good toleration.
Embodiment 7 animals are the effect to suckling piglets intestinal pH value by excrement source compound recipe probiotic bacteria solubility
The probiotic bacteria solution (hereinafter to be referred as matched group) that the compound recipe probiotic bacteria soluble powder that embodiment 1, embodiment 2, embodiment 4 are made (being correspondingly called for short respectively below test group 1,2,3) and market are buied is applied to nascent little swinery (1 age in days), be used in conjunction 10 days, relatively it is to the regulating effect of piglets intestinal pH and the miscellaneous bacteria quantity in feces.
For examination pig: nascent piglets 5 nests, institute's feed material is autogamy not containing any medicine, drinks clean tap water.
Operational approach: nascent first day, at same measure of time pig feces pH value every day.
Supply reagent thing: compound recipe probiotic bacteria soluble powder, probiotic bacteria solution (cryopreservation)
Test grouping and processing: shown in specific as follows.
The 1st group: negative matched group, does not add any medicine.
The 2nd group: embodiment 1 sample, mixed drink, 1g is watered 1L, is used in conjunction 10 days.
The 3rd group: embodiment 2 samples, mixed drink, 1g is watered 1L, is used in conjunction 10 days.
The 4th group: embodiment 4 samples, mixed drink, 1g is watered 1L, is used in conjunction 10 days.
The 5th group: medicine matched group, mixed drink, 3ml is watered 1L, is used in conjunction 10 days.
Evaluation index:
(1) death condition
(2) Gain weight
(3) feces pH value
Result of the test sees the following form shown in 2:
Table 2
As can be seen from Table 2, compare with medicine matched group, nascent piglets is fed after compound recipe probiotic bacteria soluble powder continuously, obvious to the regulating effect of intestinal pH value, obviously improves weight gain of piglets, avoids weightless.
The tolerance situation of embodiment 8 excrement source compound recipe probiotic bacteria solubilities to temperature
Inoculation compound recipe probiotic bacteria soluble powder sample (embodiment 1, embodiment 3, embodiment 4), to culture medium, is placed 12 hours respectively under 4 ℃, 25 ℃, 40 ℃ conditions, calculates total plate count.Result is as shown in table 3.
Table 3
| Sample | 4℃ | 25℃ | 40℃ |
| Embodiment 1 | 8.01×10 8 | 7.65×10 8 | 7.23×10 8 |
| Embodiment 3 | 8.21×10 8 | 7.33×10 8 | 7.30×10 8 |
| Embodiment 4 | 7.95×10 8 | 8.02×10 8 | 7.61×10 8 |
| Control drug | 6.24×10 7 | ﹤10 2 | 0 |
Result is visible, and probiotic bacteria soluble powder of the present invention is strong to the toleration of temperature, can preserve at normal temperatures, transports and use.
Above content is further description made for the present invention in conjunction with specific embodiments, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. an excrement source compound recipe probiotic bacteria soluble powder for animal, is characterized in that, according to weight percent meter, described preparation comprises following component:
Bacterial strain 10%-45%;
Garlicin 3%-10%;
Lactalbumin 3%-7%;
Quick-acting energy 0.5%-3%;
Cattle colostrums 0.2%-3%;
Vitamin 0.3%-1%;
Cryoprotective agent 1%-5%;
Excipient 26%-82%.
2. excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1, is characterized in that, according to weight percent meter, described preparation comprises following component:
Bacterial strain 15%-40%;
Garlicin 5%-8%;
Lactalbumin 4%-7%;
Quick-acting energy 1%-3%;
Cattle colostrums 0.5%-3%;
Vitamin 0.5%-1%;
Cryoprotective agent 2%-5%;
Excipient 33%-72%.
3. excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, is characterized in that,
Described bacterial strain is selected from enterococcus faecalis, enterococcus faecalis, lactoenterococcus, Lactobacillus plantarum, Lactobacillus bulgaricus, bacillus acidophilus, lactobacillus casei cheese subspecies, Lactobacillus fermenti, Lactobacillus salivarius, lactobacillus lactis, Lactobacillus buchneri, lactobacillus cellobiosas, lactobacillus thermophilus, lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, animal bifidobacteria, Bacillus licheniformis, bacillus subtilis, pediococcus acidilactici, Rhodopseudomonas palustris, produce at least five kinds in propionibacterium acide-propionici,
The described quick-acting energy adopts at least one in glyceryl monostearate, triglycerin fat acid esters, cithrol and sucrose fatty acid ester;
Described vitamin is selected from least two kinds in vitamin A, vitamin B1, vitamin B2, pellagra preventive factor, adenine phosphate, vitamin B5, vitamin B6, biotin, FA, vitamin B12, inositol, vitamin C, vitamin D, vitamin E;
Described cryoprotective agent is selected from least one in glycerol, defatted milk powder, trehalose, polyvinylpyrrolidone, mannitol, gelatin, carbomer, polymine, dibastic sodium phosphate, potassium dihydrogen phosphate, sorbitol, glycine, arginine, glutamic acid, thiourea, sodium thiosulfate, the tert-butyl alcohol;
Described excipient is selected from least one in anhydrous glucose, sucrose, soluble starch, oligomeric galactose, oligomeric xylose, oligofructose, starch.
4. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, it is characterized in that, comprise following step:
Steps A: described bacterial strain, in fluid medium, is cultivated and obtained bacterium liquid, and reconcentration filters, and obtains bacteria suspension;
Step B: by described excrement source probiotic bacteria soluble powder key component, add cryoprotective agent, vitamin, lactalbumin and cattle colostrums in bacteria suspension, obtain bacterium liquid mixture;
Step C: mixture is sprayed in the vapor tight tank that is full of carbon dioxide, carry out processed, the intermediate products after being dewatered;
Step D: by intermediate products and described garlicin, the quick-acting energy, excipient, mix 20 minutes continuously subpackage and get final product in three-dimensional motion mixer.
5. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, it is characterized in that, in described steps A: the incubation step of bacterium liquid is:
(1) described bacterial strain is inoculated into respectively in corresponding fluid medium and is cultivated, obtain seed liquor;
(2) by the seed liquor obtaining in level liquid culture medium, cultivate and to obtain primary seed solution;
(3) by the primary seed solution obtaining in secondary fluid medium, cultivate and to obtain bacterium liquid.
6. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, it is characterized in that, the temperature of step (1), (2), (3) is 36 ℃ ± 2 ℃, and rotating speed is 100-140r/min.
7. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, it is characterized in that, the incubation time of described step (2), (3) is 12-14 hour, and the incubation time of described step (1) is 20-28 hour.
8. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 1 or 2, it is characterized in that, in described steps A, the temperature of thickening filtration is-25 ℃-4 ℃.
9. preparation method as claimed in claim 4, is characterized in that, described, must keep ambient temperature at 10-15 ℃ before deviating from treatment step.
10. prepare a method for excrement source compound recipe probiotic bacteria soluble powder for animal as claimed in claim 4, it is characterized in that, in step B and step D, mixing time is 10-30 minute.
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