CN103698253A - Method for separating absorption coefficient of phytoplankton in particulate matters - Google Patents
Method for separating absorption coefficient of phytoplankton in particulate matters Download PDFInfo
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Abstract
The invention discloses a method for separating an absorption coefficient of phytoplankton in particulate matters. The method comprises the following steps: filtering by using a WhatmanGFF filter membrane to obtain particulate matters in a water environmental sample, and then, measuring the absorption coefficient of the total particulate matters; next, adsorbing, bleaching and extracting the phytoplankton on the particulate matters by using sodium hypochlorite; adsorbing 5-10 times by using ultrapure water to remove the sodium hypochlorite on the filter membrane, and remeasuring to obtain an absorption coefficient of de-pigmented particulate matters, wherein the difference value of the absorption coefficients of the total particulate matters and de-pigmented particulate matters is the absorption coefficient of the phytoplankton. Compared with the traditional method, the method can be used for well eliminating the interference to the measurement of the absorption coefficient of low-concentration phytoplankton by high-concentration de-pigmented particulate matters and improving the experimental separating precision of the absorption coefficient of the phytoplankton, causes no loss of the de-pigmented particulate matters in a treatment process, is simple and convenient to operate, high in precision and very remarkable in advantage.
Description
Technical field
The present invention relates to phytoplankton absorption coefficients in particle to carry out separated method, relate in particular to the method with sodium hypochlorite absorption bleaching phytoplankton pigment and then definite particle absorption coefficient and separated phytoplankton absorption coefficients.
Background technology
Phytoplankton and non-coloured particles thing absorb and have determined to a great extent water body changes in optical properties, affecting the transmission of optical radiation in water body distributes, and then level and vertical distribution and the migration of decision phytoplankton and submerged plant, phytoplankton and non-pigmented particle arrive phytoplankton and submerged plant photosynthesis, primary productivity and lake ecosystem type conversion to the competitive influence of spectral absorption.Phytoplankton and the absorption of non-coloured particles thing is simultaneously water body water colour parameter remote inverting basis, if the structure of current chlorophyll a bio-optical model is all to go out phytoplankton absorption coefficients based on remote sensing reflectivity or dried up radiance retrieval, then calculates and obtain in the situation that supposition specific absorptivity is constant.Therefore, how effectively and accurately the absorption coefficient of phytoplankton in separating granular, is related to quantitative identification, the quantitative remote sensing of the optics components such as phytoplankton, primary productivity and the foundation of dynamics of ecosystem model of underwater light field structure.
The Measurement of Absorption of suspended particulate substance adopts the method for filter membrane enrichment, by filter membrane being put into spectrophotometer detecting device, accepting window and proofreaies and correct the active path causing due to Multiple Scattering and increase.And developed at present some experiments method separated with numerical value for particle phytoplankton and non-pigmented particle absorption coefficient separated, wherein experimental technique mainly contains two classes: (1) is after total particulate Measurement of Absorption, with methyl alcohol, soak filter membrane and extract pigment, thereby phytoplankton is absorbed from total particulate absorbs and separated; (2) on filter membrane, directly drip liquor natrii hypochloritis or in former water sample, add liquor natrii hypochloritis phytoplankton pigment is bleached.
Above-mentioned two kinds of methods have certain application, and wherein methyl alcohol infusion method is many in ocean water body application, and sodium hypochlorite bleaching method is in muddy inland, two class water bodys are sporadicly used, but two kinds all exist obvious defect or limitation.Before a kind of method, when filter membrane is immersed in and carries out pigment extraction in methanol solution, inevitably cause the loss of part non-pigmented particle, although can again be enriched on filter membrane by cleaning, particle distributes significant change has occurred on filter membrane.In addition, when the plant concentration that swims on filter membrane is very high, pigment extracts can be not exclusively, and pigment water miscible to some or that can not be dissolved in methyl alcohol cannot be extracted, as phycobilin (phycobilins) and Eukaryotic Algae pigment etc.Therefore often cause separated phytoplankton absorption coefficients less than normal near long wave 675nm, and shortwave below 440nm is bigger than normal.Non-pigmented particle in particle is accounted for to definitely leading sample separation phytoplankton absorption coefficients out and tend to exist several-fold error.A kind of rear method, to dripping sodium hypochlorite on filter membrane, can destroy Particle distribution on filter membrane, cause its skewness, and directly add sodium hypochlorite toward former water sample, in order to guarantee Chloride Ion In Water concentration, often need a large amount of liquor natrii hypochloritises, and after filtering, be difficult to sodium hypochlorite on filter membrane to wash, causing shortwave part non-pigmented particle to absorb is often negative value, thereby causes the phytoplankton absorption coefficients of separating inaccurate.
Yet, for muddy Inland Water, because non-pigmented particle concentration is very high, in addition phytoplankton pigment form widely different, the therefore accurate phytoplankton absorption coefficients on separating granular of existing method.
Summary of the invention
The present invention seeks to set up a kind of separation method of phytoplankton absorption coefficients accurately and rapidly, be applicable to the environment water of different phytoplankton concentration and ratio, method of operating is easy simultaneously, is applicable to promotion and application.
Above-mentioned purpose of the present invention is achieved through the following technical solutions: the method for sodium hypochlorite absorption bleaching phytoplankton pigment and then separating granular absorption coefficient, particularly, after carrying out total particulate Measurement of Absorption, 1% sodium hypochlorite 3-5ml on dripping on the qualitative filter paper of GFF filter membrane periphery, allow liquor natrii hypochloritis from qualitative filter paper slowly maceration to GFF filter membrane, be placed in the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane.Utilize ultrapure water to carry out 5-10 absorption and clean sodium hypochlorite on removal filter membrane, redeterminate and calculate non-pigmented particle absorption coefficient.
According to embodiments of the invention, the present invention proposes a kind of phytoplankton absorption coefficients method in separating granular, it comprises: (1) is laid in GFF filter membrane on moistening qualitative filter paper, on the qualitative filter paper of filter membrane periphery, drip 1% liquor natrii hypochloritis 3-5ml, make liquor natrii hypochloritis from qualitative filter paper slowly maceration to described GFF filter membrane, be placed in the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane; (2) utilize ultrapure water to carry out 5-10 absorption to the GFF filter membrane obtaining in step (1) and clean, to remove residual sodium hypochlorite on filter membrane, and measure non-pigmented particle absorption coefficient; (3) based on described non-pigmented particle absorption coefficient and by the total particulate absorption coefficient of described GFF filter membrane, determine described phytoplankton absorption coefficients, wherein, the total particulate absorption coefficient of described GFF filter membrane is determined through the following steps: utilize GFF membrane filtration to obtain particle in environmental water sample, and measure the total particulate absorption coefficient of described GFF filter membrane.
In one embodiment of the invention, described 1% liquor natrii hypochloritis is diluted and is obtained by the liquor natrii hypochloritis to 8%.
In one embodiment of the invention, particularly, comprise that (a) first uses GFF membrane filtration water sample, and on spectrophotometer, measure absorbance, obtain total particulate absorption coefficient, wherein, the volume of described filtration water sample is that the turbidity based on described water sample is determined, wherein, utilize cuvette to measure the beam attenuation coefficient of described water sample, get 550nm place absorption coefficient according to determining the volume of described filtration water sample: V=500 * C (550) after following formula calculating round numbers
-0.876, wherein, V represents the volume of described filtration water sample; (b) the GFF filter membrane of determining total particulate absorption coefficient in step (a) is laid on moistening qualitative filter paper, 1% liquor natrii hypochloritis 3-5ml on dripping on the qualitative filter paper of filter membrane periphery, make described liquor natrii hypochloritis from qualitative filter paper maceration to GFF filter membrane; (c) the GFF filter membrane that has adsorbed sodium hypochlorite is placed in to the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane; (d) the GFF filter membrane extracting after bleaching is placed on dry qualitative filter paper, make qualitative filter paper adsorb to fall the sodium hypochlorite on GFF filter membrane, then 5ml ultrapure water on dripping on the qualitative filter paper of GFF filter membrane periphery, allow ultrapure water from qualitative filter paper slowly maceration to GFF filter membrane, afterwards filter membrane is placed on another dry qualitative filter paper, press aforesaid operations 5-10 time repeatedly, to adsorb completely, eliminate sodium hypochlorite on GFF filter membrane; (e) GFF filter membrane step (d) being obtained is for non-pigmented particle Measurement of Absorption.
In one embodiment of the invention, the non-pigmented particle absorption coefficient of measuring in step (e) is greater than 676-680nm place mean absorption coefficient at 671-675nm place mean absorption coefficient, but is less than 666-670nm place mean absorption coefficient.
In one embodiment of the invention, if cannot meeting at 671-675nm place mean absorption coefficient, the non-pigmented particle absorption coefficient of measuring in step (e) is greater than 676-680nm place mean absorption coefficient, but be less than 666-670nm place mean absorption coefficient, repeat to weigh step (a)-(d), until meet at 671-675nm place mean absorption coefficient, be greater than 676-680nm place mean absorption coefficient, but be less than 666-670nm place mean absorption coefficient.
In one embodiment of the invention, based on described non-pigmented particle absorption coefficient and by the total particulate absorption coefficient of described GFF filter membrane, determine that described phytoplankton absorption coefficients is definite by the total particulate absorption coefficient of described GFF filter membrane is deducted to described non-pigmented particle absorption coefficient.
Thus, according to embodiments of the invention, by sodium hypochlorite, adsorb the method for non-pigmented particle and phytoplankton absorption coefficients in bleaching phytoplankton pigment and then definite particle, specifically can operate according to the following steps:
(1) first with the appropriate water sample of GFF membrane filtration, on spectrophotometer, measure absorbance, the moistening filter membrane of GFF with ultrapure water cleaning and dipping, obtain total particulate absorption coefficient, filtering volume of water sample determines according to turbidity, specifically with cuvette, measure water sample beam attenuation coefficient, get after 550nm place absorption coefficient calculates round numbers according to following formula and be defined as filtering volume: V=500 * C (550)
-0.876.
(2) the GFF filter membrane after measuring is laid on moistening qualitative filter paper, according to the plant concentration that swims on filter membrane, on the qualitative filter paper of filter membrane periphery, drips 1% sodium hypochlorite 3-5ml, allow liquor natrii hypochloritis from qualitative filter paper slowly maceration to GFF filter membrane.
(3) the GFF filter membrane that has adsorbed sodium hypochlorite extraction agent is placed in to the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane, avoided the impact of intense light irradiation condition on phytoplankton on filter membrane.
(4) the GFF filter membrane extracting after bleaching is placed on dry qualitative filter paper, make qualitative filter paper adsorb to fall the sodium hypochlorite on GFF filter membrane, then 5ml ultrapure water on dripping on the qualitative filter paper of GFF filter membrane periphery, allow ultrapure water from qualitative filter paper slowly maceration to GFF filter membrane, afterwards filter membrane is placed on another dry qualitative filter paper, press aforesaid operations 5-10 time repeatedly, to adsorb completely, eliminate sodium hypochlorite on GFF filter membrane.
(5) the GFF filter membrane that step (4) obtains is for non-pigmented particle Measurement of Absorption.
(6) whether the non-pigmented particle absorption coefficient that determining step (5) is measured is greater than 676-680nm place mean absorption coefficient at 671-675nm place mean absorption coefficient, and is less than 666-670nm place mean absorption coefficient.
(7) if measure acquisition non-pigmented particle absorption coefficient, can not meet a
d(676-680) < a
d(671-675) < a
d(670-666) condition, repeats claim 3 step (1)-(6), until meet the demands.
Advantage of the present invention and beneficial effect:
(1) can not destroy the distribution of particle on filter membrane, guarantee that particle can be uniformly distributed on GFF filter membrane, all pigments in energy full bleaching particle, avoid the defect that in methyl alcohol infusion method, water colo(u)r cannot extract simultaneously.
(2) in implementation process, do not cause the loss of non-pigmented particle, thereby avoid artificially amplifying phytoplankton absorption coefficients, the interference of non-pigmented particle to phytoplankton absorption coefficients can be eliminated completely, even if non-pigmented particle concentration is very high in particle, also phytoplankton absorption coefficients can be accurately obtained.
(3) operation is very easy, and required cost is low: the method only needs two steps of sodium hypochlorite on 1% liquor natrii hypochloritis's dropping and ultrapure water cleaning filter membranes, and the time of processing simple sample is no more than 20 minutes, simultaneously can batch processing.Liquor natrii hypochloritis's price is very cheap, and disposal cost is low.
Accompanying drawing explanation
Fig. 1 has shown according to one embodiment of present invention, the own absorption coefficient contrast of phytoplankton, non-pigmented particle absorption coefficient and the pure algae that 90% Microcystis aeruginosa and 10% non-pigmented particle proportioning carapax et plastruw testudinis alcohol immersing extraction obtain and non-pigmented particle;
Fig. 2 has shown according to one embodiment of present invention, phytoplankton, non-pigmented particle absorption coefficient and the pure algae that under 90% Microcystis aeruginosa and 10% non-pigmented particle proportioning, sodium hypochlorite absorption bleaching extraction obtains and the absorption coefficient contrast of non-pigmented particle own;
Fig. 3 has shown according to one embodiment of present invention, the own absorption coefficient contrast of phytoplankton, non-pigmented particle absorption coefficient and the pure algae that 10% Microcystis aeruginosa and 90% non-pigmented particle proportioning carapax et plastruw testudinis alcohol immersing extraction obtain and non-pigmented particle;
Fig. 4 has shown according to one embodiment of present invention, phytoplankton, non-pigmented particle absorption coefficient and the pure algae that under 10% Microcystis aeruginosa and 90% non-pigmented particle proportioning, sodium hypochlorite absorption bleaching extraction obtains and the absorption coefficient contrast of non-pigmented particle own;
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described content of embodiment is only for the present invention is described, and should also can not limit the present invention described in claims.After the pure algae of typical algae kind Microcystis aeruginosa of take below in the eutrophication water of inland mixes with non-pigmented particle different proportion proportioning, the separated phytoplankton absorption coefficients of sodium hypochlorite bleaching is example, and this method is described further.
The required reagent of the inventive method and consumptive material:
1) organic extraction agent: 100% methanol solution, this solution is the most frequently used extraction agent of phytoplankton absorption coefficients separation in ocean water body particle.
2) contain the liquor natrii hypochloritis of 8% active chlorine:
3) ultrapure water (Millq Water): Millipore company ultrapure water machine is prepared from;
4) GFF of the Whatman company filter membrane of 25mm
Equipment needed thereby: spectrophotometer, ultrapure water machine, forcing pump, filtrator.
Operation steps:
(1) first with the appropriate water sample of GFF membrane filtration, on spectrophotometer, measure absorbance, the moistening filter membrane of GFF with ultrapure water cleaning and dipping, obtain total particulate absorption coefficient, filtering volume of water sample determines according to turbidity, specifically with cuvette, measure water sample beam attenuation coefficient, get after 550nm place absorption coefficient calculates round numbers according to following formula and be defined as filtering volume: V=500 * C (550)
-0.876.
(2) the GFF filter membrane after measuring is laid on moistening qualitative filter paper, according to the plant concentration that swims on filter membrane, on the qualitative filter paper of filter membrane periphery, drips 1% sodium hypochlorite 3-5ml, allow liquor natrii hypochloritis from qualitative filter paper slowly maceration to GFF filter membrane.
(3) the GFF filter membrane that has adsorbed sodium hypochlorite extraction agent is placed in to the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane, avoided the impact of intense light irradiation condition on phytoplankton on filter membrane.
(4) the GFF filter membrane extracting after bleaching is placed on dry qualitative filter paper, make qualitative filter paper adsorb to fall the sodium hypochlorite on GFF filter membrane, then 5ml ultrapure water on dripping on the qualitative filter paper of GFF filter membrane periphery, allow ultrapure water from qualitative filter paper slowly maceration to GFF filter membrane, afterwards filter membrane is placed on another dry qualitative filter paper, press aforesaid operations 5-10 time repeatedly, to adsorb completely, eliminate sodium hypochlorite on GFF filter membrane.
(5) the GFF filter membrane that step (4) obtains is for non-pigmented particle Measurement of Absorption.
(6) whether the non-pigmented particle absorption coefficient that determining step (5) is measured is greater than 676-680nm place mean absorption coefficient at 671-675nm place mean absorption coefficient, and is less than 666-670nm place mean absorption coefficient.
(7) if measure acquisition non-pigmented particle absorption coefficient, can not meet a
d(676-680) < a
d(671-675) < a
d(670-666) condition, repeats claim 3 step (1)-(6), until meet the demands.
Microcystis aeruginosa is typical algae kind in the eutrophication water of inland, except the chlorophyll a and chlorophyll b that contain organic solvents such as being dissolved in methyl alcohol, ethanol, is also rich in abundant algocyan.
Fig. 1 has shown phytoplankton, non-pigmented particle absorption coefficient and pure algae and the absorption coefficient contrast of non-pigmented particle own that 90% Microcystis aeruginosa and 10% non-pigmented particle proportioning carapax et plastruw testudinis alcohol immersing extraction obtain.By this figure, can determine, when in particle, phytoplankton pigment concentration is higher, methyl alcohol soaks phytoplankton on extraction particle and absorbs not exclusively, show that non-pigmented particle absorption coefficient exists weak absorption peak near 675nm, the phytoplankton absorption coefficients of extraction at this wave band lower than the absorption coefficient of pure algae own.Because methyl alcohol can not extract algocyan water colo(u)r, make non-pigmented particle near 620nm, have weak absorption peak simultaneously.In methyl alcohol immersion process, because pigment concentration is higher, pigment extracts not exclusively, and the phytoplankton absorption coefficients of extraction is starkly lower than the absorption coefficient of pure algae own, introduces obvious errors.
Fig. 2 has shown that under 90% Microcystis aeruginosa and 10% non-pigmented particle proportioning, phytoplankton, non-pigmented particle absorption coefficient and pure algae and the absorption coefficient contrast of non-pigmented particle own obtaining extracted in sodium hypochlorite absorption bleaching.By this figure, can determine, when with sodium hypochlorite absorption bleaching, the phytoplankton of separating and non-pigmented particle spectral absorptance and pure algae and the absorption coefficient of non-pigmented particle own are very approaching, even if illustrate when in particle, phytoplankton pigment concentration is higher, sodium hypochlorite can compare full bleaching and fall phytoplankton on particle, can not cause phytoplankton absorption coefficients on the low side, more satisfactory for separating of high concentration phytoplankton absorption coefficients on particle.
Fig. 3 has shown phytoplankton, non-pigmented particle absorption coefficient and pure algae and the absorption coefficient contrast of non-pigmented particle own that 10% Microcystis aeruginosa and 90% non-pigmented particle proportioning carapax et plastruw testudinis alcohol immersing extraction obtain.By this figure, can determine, when in particle, phytoplankton pigment concentration is very low, methyl alcohol soaks phytoplankton absorption on extraction particle can cause non-pigmented particle loss, cause and separate non-pigmented particle absorption coefficient and be starkly lower than non-pigmented particle actual absorption coefficient, thereby cause separated phytoplankton absorption coefficients apparently higher than the actual absorption coefficient of pure algae, introduce obvious errors.
Fig. 4 has shown that under 10% Microcystis aeruginosa and 90% non-pigmented particle proportioning, phytoplankton, non-pigmented particle absorption coefficient and pure algae and the absorption coefficient contrast of non-pigmented particle own obtaining extracted in sodium hypochlorite absorption bleaching.By this figure, can determine, when with sodium hypochlorite absorption bleaching, the phytoplankton of separating and non-pigmented particle spectral absorptance and pure algae and the absorption coefficient of non-pigmented particle own are very approaching, even if illustrate when in particle, phytoplankton pigment concentration is very low, sodium hypochlorite bleaching can not cause non-pigmented particle loss and phytoplankton absorption coefficients on the low side, more satisfactory for separating of low concentration phytoplankton absorption coefficients on particle.
Claims (6)
1. a method for phytoplankton absorption coefficients in separating granular, is characterized in that comprising:
(1) GFF filter membrane is laid on moistening qualitative filter paper, on the qualitative filter paper of filter membrane periphery, drip 1% liquor natrii hypochloritis 3-5ml, make liquor natrii hypochloritis from qualitative filter paper slowly maceration to described GFF filter membrane, be placed in the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane;
(2) utilize ultrapure water to carry out 5-10 absorption to the GFF filter membrane obtaining in step (1) and clean, to remove residual sodium hypochlorite on filter membrane, and measure non-pigmented particle absorption coefficient;
(3) based on described non-pigmented particle absorption coefficient and by the total particulate absorption coefficient of described GFF filter membrane, determine described phytoplankton absorption coefficients,
Wherein, the total particulate absorption coefficient of described GFF filter membrane is determined through the following steps:
Utilize GFF membrane filtration to obtain particle in environmental water sample, and measure the total particulate absorption coefficient of described GFF filter membrane.
2. method according to claim 1, is characterized in that, described 1% liquor natrii hypochloritis is diluted and obtained by the liquor natrii hypochloritis to 8%.
3. method according to claim 2, is characterized in that, comprises
(a) first use GFF membrane filtration water sample, and on spectrophotometer, measure absorbance, obtain total particulate absorption coefficient, wherein, the volume of described filtration water sample is that the turbidity based on described water sample is determined, wherein, utilize cuvette to measure the beam attenuation coefficient of described water sample, get 550nm place absorption coefficient according to determining the volume of described filtration water sample: V=500 * C (550) after following formula calculating round numbers
-0.876, wherein, V represents the volume of described filtration water sample;
(b) the GFF filter membrane of determining total particulate absorption coefficient in step (a) is laid on moistening qualitative filter paper, 1% liquor natrii hypochloritis 3-5ml on dripping on the qualitative filter paper of filter membrane periphery, make described liquor natrii hypochloritis from qualitative filter paper maceration to GFF filter membrane;
(c) the GFF filter membrane that has adsorbed sodium hypochlorite is placed in to the dark surrounds plant pigment 10-15 minute that carries out swimming on sodium hypochlorite absorption bleaching filter membrane;
(d) the GFF filter membrane extracting after bleaching is placed on dry qualitative filter paper, make qualitative filter paper adsorb to fall the sodium hypochlorite on GFF filter membrane, then 5ml ultrapure water on dripping on the qualitative filter paper of GFF filter membrane periphery, allow ultrapure water from qualitative filter paper slowly maceration to GFF filter membrane, afterwards filter membrane is placed on another dry qualitative filter paper, press aforesaid operations 5-10 time repeatedly, to adsorb completely, eliminate sodium hypochlorite on GFF filter membrane;
(e) GFF filter membrane step (d) being obtained is for non-pigmented particle Measurement of Absorption.
4. method according to claim 3, is characterized in that, the non-pigmented particle absorption coefficient of measuring in step (e) is greater than 676-680nm place mean absorption coefficient at 671-675nm place mean absorption coefficient, but is less than 666-670nm place mean absorption coefficient.
5. method according to claim 3, it is characterized in that, if the non-pigmented particle absorption coefficient of measuring in step (e) cannot meet at 671-675nm place mean absorption coefficient, be greater than 676-680nm place mean absorption coefficient, but be less than 666-670nm place mean absorption coefficient
Repeat to weigh step (a)-(d), be greater than 676-680nm place mean absorption coefficient until meet at 671-675nm place mean absorption coefficient, but be less than 666-670nm place mean absorption coefficient.
6. according to the method for claim 5, it is characterized in that, based on described non-pigmented particle absorption coefficient and by the total particulate absorption coefficient of described GFF filter membrane, determine that described phytoplankton absorption coefficients is definite by the total particulate absorption coefficient of described GFF filter membrane is deducted to described non-pigmented particle absorption coefficient.
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| CN114624152A (en) * | 2022-05-16 | 2022-06-14 | 生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心 | Method for testing organic carbon source of water body particles and related equipment |
| CN116952906A (en) * | 2023-09-20 | 2023-10-27 | 南京航天宏图信息技术有限公司 | Water body health state assessment method and device, electronic equipment and storage medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104132901A (en) * | 2014-08-18 | 2014-11-05 | 中国科学院南京地理与湖泊研究所 | Method for improving absorption and determination precision of suspended particulate matters in water body |
| CN104132901B (en) * | 2014-08-18 | 2016-08-24 | 中国科学院南京地理与湖泊研究所 | A kind of method improving Suspended Particles in Water absorption measurement precision |
| CN114624152A (en) * | 2022-05-16 | 2022-06-14 | 生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心 | Method for testing organic carbon source of water body particles and related equipment |
| CN116952906A (en) * | 2023-09-20 | 2023-10-27 | 南京航天宏图信息技术有限公司 | Water body health state assessment method and device, electronic equipment and storage medium |
| CN116952906B (en) * | 2023-09-20 | 2024-01-12 | 南京航天宏图信息技术有限公司 | Water body health state assessment method and device, electronic equipment and storage medium |
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| CN103698253B (en) | 2016-01-20 |
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