CN103695351A - Acinetobacter baumannii and application thereof - Google Patents
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Abstract
The invention discloses acinetobacter baumannii and application thereof. The acinetobacter baumannii is named as Acinetobacter baumannii DD1 which has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013560 and is preserved in the CCTCC in Wuhan University of Wuhan in China on November 8th, 2013. The acinetobacter baumannii is inoculated into waste water containing dioxane or introduced into an inorganic salt culture medium of dioxane waste gas to be cultured. As a result, the dioxane can be completely degraded, and contaminants such as toluene, phenol and tetrahydrofuran can be degraded. The acinetobacter baumannii is a non-fermentation-type aerobiotic gram-negative dyeing bacterium which can grow by taking the dioxane as a sole carbon source and energy source and is capable of effectively degrading the dioxane substrate at the same time. The acinetobacter baumannii can be used for degrading various contaminants such as toluene, phenol and tetrahydrofuran. Thus, a foundation for engineering application of purifying wastewater or waste gas containing the dioxane via a biological method is laid.
Description
Technical field
The present invention relates to a kind of degradation method of diox, be specifically related to a kind of Acinetobacter baumannii (Acinetobacter baumannii) and application thereof.
Background technology
1,4-dioxane (1,4-dioxane), another name diox, having well water-solublely, is a kind of good organic solvent, can be compatible with ethanol, ether, acetone, phenol etc., be widely used in the industries such as paint, dyestuff, pharmacy, be also applied in the consuming product such as food, makeup and washing composition.Diox is irritant to skin, eye and respiratory system, can in human body, accumulate, liver, kidney and neural system are had to serious infringement, chronic progressive poisoning can cause the diseases such as uremia, renal failure, acute poisoning may cause death, by Environmental Protection Agency (U.S.EPA) and the international tumor research center of the World Health Organization (IARC), is classified as B2 level (possible) mankind carcinogens.
The extensive application of diox, causes the pollution of surface water and groundwater day by day serious.At present, the pollution of diox in the underground water of the U.S., Canada and a plurality of developed countries such as Japanese and landfill yard, all detected; In near the underground water of the refuse landfill Canadian Ottawa, Lake Ontario and Canada, detect high density diox; China the Yellow River also detects a large amount of dioxs, and detects the also accumulation of Chu diox in the indigenous fish internal organ of Lanzhou section.More there are some researches show, even if the existence of diox also detected in hard to get to arctic frozen soil and underground water.Therefore, the removal of surface water and groundwater Zhong diox is very urgent, needs a kind of effective method badly and is removed.
For the method for removing diox, be mainly chemical oxidization method both at home and abroad at present, comprise ozone, UV illumination, hydrogen peroxide and Fenton oxidation etc., these methods all have advantages of separately, but this class methods processing cost is too high, are not suitable for the environmental pollution improvement of diox.Due to diox, there is the physico-chemical properties such as cyclic ether structure, C-O energy-rich bond, low Henry's constant and low octanol-water partition coefficient, be once classified as " being difficult for degradable chemical ".
1991, German scholar Bemhardt etc. isolated first the bacterial strain Rhodococcus ruber219 with diox degradation capability Cong the mud in diox chemical plant, have realized for the first time biological degradation diox.Thereby increasing investigator starts to pay close attention to the research that biological process is processed diox.1993, Burback etc. had and isolate the bacterial strain Mycobacterium vaccae that a strain has diox degradation capability, but this bacterial strain diox degradation capability is limited and can not continue growth.The mycobacterium PH-06 of the report such as Young-Mo Kim can degrade 90% by 1000mg/L diox in 15 days, but degradation cycle is long.The isolated fungi Cordyceps such as Nakamiya sinensis also can be using diox as sole carbon source and the energy continue growth.Report diox degradation bacteria also has Pseudonocardia dioxanivorans CB1190, Pseudonocardia B5, Bacillus pumilus D4 and Xanthobacter D7 etc. at present.
Summary of the invention
The invention provides a kind of Acinetobacter baumannii (Acinetobacter baumannii) and application thereof, (degradation rate of Acinetobacter baumannii) Dui diox reaches more than 99.9% this Acinetobacter baumannii, the pollutents such as toluene, phenol, tetrahydrofuran (THF) of can also degrading.
An Acinetobacter baumannii, called after Acinetobacter baumannii DD1(Acinetobacter baumanniiDD1), preserving number is CCTCC NO:M2013560.
Acinetobacter baumannii DD1(Acinetobacter baumannii DD1 of the present invention) on November 8th, 2013, be preserved in the Chinese Typical Representative culture collection center that is positioned at Wuhan, China Wuhan University.
Acinetobacter baumannii DD1 of the present invention is collected in the second pond active sludge of sewage work, is shaped as rod-short, and size is 1.0～1.5 * 1.5～2.5 μ m, and atrichia, without gemma; 30 ℃ of solid plate substratum, cultivate after 48h, single bacterium colony is spherical shape, smooth moistening, and lawn is along line growth; Gramstaining is negative, and oxydase, catalase are tested negative; Gelatin, Citrate trianion, nitrate reduction are tested positive; Kantlex, rifomycin are had to resistance, to tsiklomitsin non-resistant; Salinity is greater than 4% above poor growth.
The present invention also provide a kind of as described in Acinetobacter baumannii application in diox in degraded.
This application is specially: described Acinetobacter baumannii is seeded in waste water, the minimal medium that contains diox that contains diox or the minimal medium that passes into diox waste gas and is cultivated, degraded diox.
Described Acinetobacter baumannii DD1(Acinetobacter baumannii DD1) can utilize diox as unique carbon source and energy substance growth and breeding, diox is mineralized into CO
2and H
2o.Under pure culture condition, this bacterium can be degradable by the 100mg/L diox in minimal medium in 42h.
Further preferably, described cultivation is 5.0～8.0 in pH value, temperature is to carry out in the scope of 25 ℃～40 ℃.More preferably in pH value, be 7.0, temperature is to carry out in the scope of 32 ℃.
Further preferably, the starting point concentration of described Fei water Zhong diox is 100mg/L～500mg/L; The starting point concentration of the described minimal medium Zhong diox that contains diox is 100mg/L～500mg/L; The described starting point concentration that passes into the minimal medium Zhong diox of diox waste gas is 100mg/L～500mg/L.More preferably be 200mg/L.
The described starting point concentration that passes into the minimal medium Zhong diox of diox waste gas is that 100mg/L～500mg/L refers to: in minimal medium, diox starting point concentration refers to pass into and in diox waste gas, is dissolved in diox starting point concentration in inorganic salt culture medium, therefore the intake of ， diox waste gas is measured with the required starting point concentration of minimal medium Zhong diox.
Further preferably, incubation time is 20～50h, more preferably 42h.
Further preferably, described minimal medium (MSM) comprises following material: 3.5g Na
2hPO
42H
2o, 1.0g KH
2pO
4, 0.5g (NH
4)
2sO
4, 0.1g MgCl
26H
2o, 0.05g Ca (NO
3)
2, be dissolved in 1000mL water, add 1ml trace element solution, regulate pH to 7.0～7.2.The minimal medium that contains diox or to pass into the medium component part of minimal medium of You diox waste gas identical.
Described trace element solution consists of FeSO
47H
2o1.0g, CuSO
45H
2o0.02g, H
3bO
30.014g, MnSO
44H
2o0.10g, ZnSO
47H
2o0.10g, Na
2moO
42H
2o0.02g, CoCl
26H
2o0.02g, is dissolved in 1000mL water.
Acinetobacter baumannii of the present invention can also be for degradation of phenol, toluene, tetrahydrofuran (THF) or o-Xylol.
Described Acinetobacter baumannii is seeded in the minimal medium that contains phenol, toluene or tetrahydrofuran (THF) and is cultivated, degradation of phenol, toluene or tetrahydrofuran (THF).Other degradation conditions are the same with degraded diox, and degradation time is different, and the degradation rate of bacterial strain Degradation of Phenol 20h of the present invention reaches 99.9%, and the degradation rate of degradation of toluene 24h is reached to 50%, and the degradation rate of tetrahydrofuran (THF) degraded 48h is reached to 99.9%.
Acinetobacter baumannii of the present invention is also for the propyl carbinol of degrading, normal hexane, ethanol or benzene, and degradation rate all reaches 99.9%.The method of degradation treatment is the same with degraded diox, and degradation time is different, and the best degradation time of propyl carbinol is 24h, and the best degradation time of normal hexane is 108h, and the best degradation time of ethanol is 24h, and the best degradation time of benzene is 24h.
Beneficial effect of the present invention is:
The invention provides the Acinetobacter baumannii of a strain energy efficient degradation diox, this bacterial strain is aerobic non-fermented type Gram-negative bacterium, can take diox as sole carbon source and energy growth while this substrate of efficient degradation, and degradation rate reaches more than 99%; This bacterial strain multiple pollutants such as toluene, phenol, tetrahydrofuran (THF) of degrading; The engineering that the present invention contains diox waste water or waste gas for employing biological method purification is applied and is laid a good foundation.
Accompanying drawing explanation
Fig. 1 is Acinetobacter baumannii DD1 degraded diox and thalli growth situation;
Fig. 2 be different pH for cell concentration with the impact of diox degradation rate;
Fig. 3 is diox concentration changes with time situation Xia differing temps;
Fig. 4 is different diox starting point concentration Xia diox concentration changes with time situations;
Fig. 5 is Acinetobacter baumannii DD1 degradation of phenol and growth situation;
Fig. 6 is Acinetobacter baumannii DD1 tetrahydrofuran-degrading and growth situation.
Embodiment
Below in conjunction with specific embodiment, invention is described further, but protection scope of the present invention is not limited in this:
Minimal medium (MSM) comprises following material: 3.5g Na
2hPO
42H
2o, 1.0g KH
2pO
4, 0.5g (NH
4)
2sO
4, 0.1g MgCl
26H
2o, 0.05g Ca (NO
3)
2, be dissolved in 1000mL water, add 1ml trace element solution, regulate pH to 7.0～7.2.
Trace element solution consists of FeSO
47H
2o1.0g, CuSO
45H
2o0.02g, H
3bO
30.014g, MnSO
44H
2o0.10g, ZnSO
47H
2o0.10g, Na
2moO
42H
2o0.02g, CoCl
26H
2o0.02g, is dissolved in 1000mL water.
Separation and the evaluation of embodiment 1:Acinetobacter baumannii DD1
(1) sample collecting and domestication
The active sludge of collection in worksite Hangzhou Qi Ge sewage work second pond, take diox as sole carbon source and the energy, tames, enrichment.After several months, the active sludge after enrichment, is inoculated in the 250mL triangular pyramidal bottle that contains 100mL MSM substratum, usings diox as sole carbon source and energy substance, continues cultivation, enrichment.Experiment needs constant temperature (30 ± 1 ℃), and remains under aerobic condition and carry out.
(2) isolation and identification for strains
Mixed bacteria liquid through the enrichment of repeatedly going down to posterity is carried out to gradient dilution, by the form of coating method, be inoculated into and only using on the inorganic salt nutrient agar of diox as sole carbon source, be placed in constant incubator (30 ℃) and cultivate.Picking list bacterium colony.Single bacterium colony is repeatedly rule after separation, then takes back the MSM substratum of Han diox 100mg/L) in cultivate, test its whether have degraded diox ability.Choose the bacterial classification with degradation capability and carry out further separation and purification, until isolate the bacterial strain DD1 with diox degradation capability.
Bacterial strain is shaped as rod-short, and size is 1.0～1.5 * 1.5～2.5 μ m, and atrichia, without gemma; 30 ℃ of solid plate substratum, cultivate after 48h, single bacterium colony is spherical shape, faint yellow, smooth moistening, and lawn is along line growth; Gramstaining is negative; Gelatin, Citrate trianion, nitrate reduction are tested positive; Oxydase, catalase are tested negative; Kantlex, rifomycin are had to resistance, to tsiklomitsin non-resistant; Salinity is greater than 4% above poor growth.
The Physiological-biochemical Characters of the acinetobacter that above-mentioned feature and the outstanding Bacteria Identification handbook > > of < < uncle edit and record matches.This bacterial strain is through Biolog microorganism identification and 16S rDNA homology analysis, mycology feature in conjunction with above Physiology and biochemistry, be accredited as Acinetobacter baumannii, the complete genome sequence of 16S rDNA is as shown in SEQ ID NO:1, and GenBank accession number is KF713537.
By this Acinetobacter baumannii called after Acinetobacter baumannii DD1(Acinetobacter baumannii DD1), on November 8th, 2013, be deposited in the Chinese Typical Representative culture collection center that is positioned at Wuhan, China Wuhan University, preserving number is CCTCC NO:M2013560.
The characteristic of embodiment 2:Acinetobacter baumannii DD1 degraded diox
(1) diox, as the sole carbon source of Acinetobacter baumannii DD1, is inoculated Acinetobacter baumannii DD1 thalline to 100mL minimal medium, initial thalline OD
600be 0.01; Adding diox to make initial diox concentration is 100mg/L.Being placed in temperature is 30 ℃, in the shaking table that revolution is 130r/min, cultivate, and regularly sampling, while cultivating 42h, the OD value of bacterial strain reaches maximum value 0.183, the results are shown in Figure 1.
As shown in Figure 1, along with the prolongation of time, the concentration of bacterial classification also constantly increases.The present embodiment explanation degradation bacteria Acinetobacter baumanniiDD1 can utilize diox to carry out biological self reproducing as sole carbon source and the energy, and has the ability of efficient degradation diox.
(2) with NaOH or HCl solution, regulate minimal medium to different pH values (5.0,6.0,6.5,7.0,7.5,8.0), under the condition that is 100mg/L in initial diox concentration, access bacterial strain makes initial thalline OD
600be 0.01.Sample is placed in to shaking culture in 30 ℃, 130r/min constant-temperature table, samples after cultivating 35h, the results are shown in Figure 2.
As seen from Figure 2, in the scope of pH5.0～8.0, the microorganism diox of all can degrading preferably, and follow the growth of cell concn; Along with the continuous increase of pH, after taking the lead in raising, bacterial strain concentration and diox degraded decline, and the optimal pH of degraded is 7.0.
(3), in the minimal medium that is 100mg/L in initial diox concentration, access bacterial strain makes initial thalline OD
600be 0.01.The temperature of each sample is set to respectively to 25 ℃, 30 ℃, 32 ℃, 37 ℃, 40 ℃, is placed in shaking culture in 130r/min constant-temperature table, timing sampling, the residual concentration of mensuration diox, the results are shown in Figure 3.As shown in Figure 3, in the scope of 25 ℃～40 ℃, bacterial strain all can be grown, and degradation rate is along with the rising of temperature has a process that first rises and decline afterwards, and in the time of 32 ℃, degradation rate reaches maximum.
(4) diox starting point concentrations are respectively in the minimal medium of 100mg/L, 200mg/L, 300mg/L, 400mg/L, 500mg/L, and access bacterial strain makes initial thalline OD
600be 0.01, sample is placed in to shaking culture in 30 ℃, 130r/min constant-temperature table, regularly sampling, the results are shown in Figure 4.As shown in Figure 4, except 500mg/L, can be degradable, degradation rate is along with the rising of concentration has a process that first rises and decline afterwards, and wherein, the in the situation that of 200mg/L, degradation rate is the fastest.
Embodiment 3:Acinetobacter baumannii DD1 degradation of phenol and tetrahydrofuran (THF)
Using phenol and tetrahydrofuran (THF) respectively as sole carbon source and the energy of Acinetobacter baumannii DD1, is all in the minimal medium of 100mg/L at starting point concentration, and access bacterial strain makes initial thalline OD
600be 0.01, sample is placed in to shaking culture in 30 ℃, 130r/min constant-temperature table, regularly sampling, the results are shown in Figure 5, Fig. 6.
From the result of Fig. 5, along with the prolongation of incubation time, the concentration of bacterial classification increases, and the concentration of phenol reduces, and when incubation time surpasses 20h, phenol is degraded completely substantially;
From the result of Fig. 6, along with the prolongation of incubation time, the concentration of bacterial classification increases, and the concentration of tetrahydrofuran (THF) reduces, and when incubation time reaches 70h, tetrahydrofuran (THF) is degraded completely substantially;
Embodiment 4:Acinetobacter baumannii DD1 substrate broad spectrum degraded situation
In minimal medium, add respectively various substrates (substrate of take respectively in table 1 is single carbon source), dosage sees the following form 1, and access bacterial strain makes initial thalline OD
600be 0.01, sample is placed in to shaking culture in 30 ℃, 130r/min constant-temperature table, period sampling measuring, result shows, bacterial strain cannot directly utilize the pollutents such as trichloromethane, trieline, trichoroacetic acid(TCA), octane-iso, and the substrate situation that can directly utilize sees the following form 1.
Table 1Acinetobacter baumannii DD1 substrate broad spectrum degraded situation
| Material | Boiling point | Add concentration (mg/L) | Degradation time | Degradation rate |
| Phenol | 181.9℃ | 94.11 | 20h | 99.9% |
| Toluene | 110.6℃ | 92.14 | 24h | 50% |
| Propyl carbinol | 117.25℃ | 74.12 | 24h | 99.9% |
| Diox | 101.3℃ | 88.11 | 40h | 99.9% |
| Tetrahydrofuran (THF) | 66℃ | 72.11 | 70h | 99.9% |
| Normal hexane | 68.74℃ | 86.17 | 108h | 99.9% |
| Ethanol | 78.5℃ | 46.07 | 24h | 99.9% |
| Benzene | 80.1℃ | 78 | 24h | 99.9% |
| O-Xylol | 140℃ | 68.8 | 60h | 40% |
As shown in Table 1, Acinetobacter baumannii DD1 phenol of the present invention, propyl carbinol, diox, tetrahydrofuran (THF), normal hexane, ethanol and benzene all have good degradation effect, and under its best incubation time separately, degradation rate all can reach 99.9%.
Claims (8)
1. an Acinetobacter baumannii, is characterized in that, called after Acinetobacter baumannii DD1(Acinetobacter baumannii DD1), preserving number is CCTCC NO:M2013560.
2. the application of Acinetobacter baumannii in degraded diox as claimed in claim 1.
3. the application of Acinetobacter baumannii in degraded diox as claimed in claim 2, it is characterized in that, described Acinetobacter baumannii is seeded in waste water, the minimal medium that contains diox that contains diox or the minimal medium that passes into diox waste gas and is cultivated, degraded diox.
4. Acinetobacter baumannii application in diox in degraded as claimed in claim 3, is characterized in that, described cultivation is 5.0～8.0 in pH value, temperature is to carry out in the scope of 25 ℃～40 ℃.
5. the application of Acinetobacter baumannii in degraded diox as claimed in claim 3, is characterized in that, the starting point concentration of described Fei water Zhong diox is 100mg/L～500mg/L; The starting point concentration of the described minimal medium Zhong diox that contains diox is 100mg/L～500mg/L; The described starting point concentration that passes into the minimal medium Zhong diox of diox waste gas is 100mg/L～500mg/L.
6. the application of Acinetobacter baumannii in degraded diox as claimed in claim 3, is characterized in that, described minimal medium comprises following material: 3.5g Na
2hPO
42H
2o, 1.0g KH
2pO
4, 0.5g (NH
4)
2sO
4, 0.1g MgCl
26H
2o, 0.05g Ca (NO
3)
2, be dissolved in 1000mL water, add 1ml trace element solution, regulate pH to 7.0～7.2.
7. the application of Acinetobacter baumannii in degraded diox as claimed in claim 6, is characterized in that, described trace element solution consists of: FeSO
47H
2o1.0g, CuSO
45H
2o0.02g, H
3bO
30.014g, MnSO
44H
2o0.10g, ZnSO
47H
2o0.10g, Na
2moO
42H
2o0.02g, CoCl
26H
2o0.02g, is dissolved in 1000mL water.
8. the application of Acinetobacter baumannii in degradation of phenol, toluene, propyl carbinol, normal hexane, ethanol, benzene, tetrahydrofuran (THF) or o-Xylol as claimed in claim 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104528949A (en) * | 2014-12-28 | 2015-04-22 | 国家电网公司 | Process for treating desulfurization wastewater of power plant |
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| CN109628337A (en) * | 2018-11-20 | 2019-04-16 | 江苏宜裕环保科技有限公司 | One plant of phenol degradation by bacterial strain with high efficiency and its application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102168038A (en) * | 2010-12-01 | 2011-08-31 | 浙江工业大学 | Xanthobacter sp. D7 capable of degrading dioxane and application thereof |
| CN102433272A (en) * | 2011-11-10 | 2012-05-02 | 浙江工业大学 | Xanthobacter flavus DT8 and the use thereof for degrading cyclic ethers |
-
2013
- 2013-12-18 CN CN201310700768.5A patent/CN103695351B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102168038A (en) * | 2010-12-01 | 2011-08-31 | 浙江工业大学 | Xanthobacter sp. D7 capable of degrading dioxane and application thereof |
| CN102433272A (en) * | 2011-11-10 | 2012-05-02 | 浙江工业大学 | Xanthobacter flavus DT8 and the use thereof for degrading cyclic ethers |
Non-Patent Citations (2)
| Title |
|---|
| SIMON VAINBERG, ET AL.: "Biodegradation of Ether Pollutants by Pseudonocardia sp. Strain ENV478", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
| 周玉央等: "食油假单胞菌DT4 菌株对四氢呋喃(THF)的降解特性", 《环境科学》 * |
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| CN104528949A (en) * | 2014-12-28 | 2015-04-22 | 国家电网公司 | Process for treating desulfurization wastewater of power plant |
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