CN103695324A - Single-cell protein production method from waste watermelon peel - Google Patents
Single-cell protein production method from waste watermelon peel Download PDFInfo
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- CN103695324A CN103695324A CN201310477391.1A CN201310477391A CN103695324A CN 103695324 A CN103695324 A CN 103695324A CN 201310477391 A CN201310477391 A CN 201310477391A CN 103695324 A CN103695324 A CN 103695324A
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- Prior art keywords
- cell protein
- single cell
- watermelon rind
- waste
- production
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- Pending
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- 241000219109 Citrullus Species 0.000 title claims abstract description 27
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 title claims abstract description 27
- 108010027322 single cell proteins Proteins 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 230000014616 translation Effects 0.000 title claims abstract description 6
- 239000002699 waste material Substances 0.000 title abstract description 6
- 241000283690 Bos taurus Species 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 17
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000004202 carbamide Substances 0.000 claims abstract description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000002054 inoculum Substances 0.000 claims abstract description 4
- 239000000835 fiber Substances 0.000 claims abstract description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000000725 suspension Substances 0.000 abstract description 3
- 235000019750 Crude protein Nutrition 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000011177 media preparation Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
废弃西瓜皮制造单细胞蛋白发明涉及废弃西瓜皮制造单细胞蛋白的方法和单细胞蛋白生产的工艺领域；特别涉及废弃西瓜皮制造单细胞蛋白和单细胞蛋白生产的工艺领域中发酵的最佳工艺条件。本发明提供废弃西瓜皮制造单细胞蛋白的方法和工艺：菌种选取-培养基的配制-酵母菌悬液的制备-尿素加入量、酵母菌液接种量、发酵时间、初始pH；废弃西瓜皮制造单细胞蛋白的生产工艺条件：尿素2.0g，菌种接种量7.5ml，培养时间48h，初始pH5.0。发酵后的产品粗蛋白由10.5％提高到25.6％，粗纤维由1.7％降低到0.5％，粗灰分由2.5％提高到7.1％。The invention relates to the method of producing single-cell protein from waste watermelon rind and the technical field of single-cell protein production; in particular, it relates to the best fermentation process in the field of production of single-cell protein from waste watermelon rind and the production of single-cell protein condition. The invention provides a method and process for producing single-cell protein from waste watermelon rind: selection of strains - preparation of culture medium - preparation of yeast suspension - urea addition, yeast liquid inoculation amount, fermentation time, initial pH; waste watermelon rind The production process conditions for the production of single-cell protein: urea 2.0g, strain inoculum 7.5ml, culture time 48h, initial pH 5.0. The crude protein of the fermented product increases from 10.5% to 25.6%, the crude fiber decreases from 1.7% to 0.5%, and the crude ash increases from 2.5% to 7.1%.
Description
Technical field
Patent of the present invention relates to Watermelon rind and utilizes field, particularly relates to discarded Watermelon rind and manufactures the optimum process condition fermenting in the technology field of single cell protein and single cell protein production.
Background technology
Single cell protein also claims microbial proteinous, refers to that bacterium, fungi and some lower algae are biological, in its process of growth, utilizes various matrix under optimum conditions, cultivates the individuality of unicellular or filamentous microorganism and the tropina that obtains.The contained nutritive substance of single cell protein is very abundant, and with dry weight basis, protein content can reach 42%～75%, and its amino acid composition is complete, and the essential amino acids content such as Methionin are higher, and also rich vitamin, has become one of feed protein supply approach.
Watermelon is annual climing property herbaceous plant, and all there is cultivating watermelon in Chinese north and south, and wherein Guangxi is the domestic and export base of the larger aspermous watermelon in China South China.The not fatty and cholesterol of watermelon, contains the materials such as a large amount of glucose, Argine Monohydrochloride, oxysuccinic acid and vitamins C.Yet most people, after eating up melon pulp, all can be thrown away when rubbish with handgrip Watermelon rind, not only makes potential resource waste, and also causes serious environmental pollution.Therefore, utilize discarded Watermelon rind to manufacture single cell protein, improve the comprehensive utilization value of discarded Watermelon rind, improve its added value, open up new feedstuff protein source, reduce the wasting of resources, eliminate environmental pollution, be of great practical significance.
Present method is that the discarded Watermelon rind of utilization is main raw material, select Angel fodder yeast bacterium to carry out produce unicellular albumen by fermentation in solid state, the content of crude protein before and after fermentation of take is leading indicator, obtains best zymotechnique, for Watermelon rind manufacture order cell protein suitability for industrialized production provides reference.
Summary of the invention
Patent of the present invention relates to discarded Watermelon rind and manufactures the method for single cell protein and the technology field that single cell protein is produced; Be particularly related to discarded Watermelon rind and manufacture the optimum process condition fermenting in the technology field of single cell protein and single cell protein production.Patent of the present invention provides discarded Watermelon rind to manufacture the Method and process of single cell protein: preparation-urea add-on of preparation-yeast suspension of choose-substratum of bacterial classification, yeast liquid inoculum size, fermentation time, initial pH; Discarded Watermelon rind is manufactured the manufacturing condition of single cell protein: urea 2.0g, bacterial classification inoculum size 7.5ml, incubation time 48h, initial pH5.0.Product coarse albumen after fermentation brings up to 25.6% by 10.5%, and robust fibre is reduced to 0.5% by 1.7%, and coarse ash brings up to 7.1% by 2.5%.
Embodiment
One, preparation substratum
(1) PDA solid medium: 200g peeling potato, 15g agar, distilled water 1000ml, PH nature.
(2) PDA liquid nutrient medium: 200g peeling potato, distilled water 1000ml, pH nature.
(3) Watermelon rind solid medium: Watermelon rind squeezing slurry 50g, Wheat bran 6g, magnesium sulfate 0.36g, potassium primary phosphate 0.45g.121 ℃ of sterilizing 20min.
Two, the preparation of yeast suspension
By pure yeast, a little is spread in the triangular flask of the PDA liquid nutrient medium that 50ml is housed, and at 140r/min, under the condition of 40 ℃, shaking table is cultivated 30min.Then line in PDA solid medium and cultivate 2d, in picking list bacterium colony access PDA liquid nutrient medium, carry out enlarged culturing, by plate count, cell count is about 108/ml.
Three, fermentation
In Watermelon rind solid medium, add respectively 7.5ml Angel fodder yeast bacterium liquid, 2g.0 gram of urea, arranges initial pH5.0, at 28 ℃ of ferment at constant temperature.In fermenting process, stir twice every day, and churning time 3min cultivates 48h.
Four, finished product extracts
After 48h, get nutrient solution with whizzer with the centrifugal 15min of 3800r/min, under 105 ℃ of conditions, dry to constant weight and obtain single cell protein product.
Claims (3)
1. a single cell protein product, it is characterized in that utilizing discarded Watermelon rind to do raw material, add urea, initial pH is set, utilizes at a certain temperature Angel fodder yeast, ferment at constant temperature processing and fabricating forms, this single cell protein product coarse albumen brings up to 25.6% by 10.5%, robust fibre is reduced to 0.5% by 1.7%, and coarse ash brings up to 7.1% by 2.5%, can do feedstuff protein source.
2. single cell protein fabrication processing according to claim 1, it is characterized in that discarded Watermelon rind single cell protein production has four important technical process, each technique has intrinsic method and condition, according to method steps, operates and can obtain single cell protein product.
3. according to claim 1 and discarded Watermelon rind single cell protein production claimed in claim 2 technical process, fermented liquid fermentation has best processing condition, and they are urea add-on, yeast liquid inoculum size, fermentation time, initial pH.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310477391.1A CN103695324A (en) | 2013-10-01 | 2013-10-01 | Single-cell protein production method from waste watermelon peel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310477391.1A CN103695324A (en) | 2013-10-01 | 2013-10-01 | Single-cell protein production method from waste watermelon peel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103695324A true CN103695324A (en) | 2014-04-02 |
Family
ID=50356988
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310477391.1A Pending CN103695324A (en) | 2013-10-01 | 2013-10-01 | Single-cell protein production method from waste watermelon peel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103695324A (en) |
-
2013
- 2013-10-01 CN CN201310477391.1A patent/CN103695324A/en active Pending
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| C06 | Publication | ||
| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140402 |