CN103690273A - In vivo bioreactor - Google Patents
In vivo bioreactor Download PDFInfo
- Publication number
- CN103690273A CN103690273A CN201310586372.2A CN201310586372A CN103690273A CN 103690273 A CN103690273 A CN 103690273A CN 201310586372 A CN201310586372 A CN 201310586372A CN 103690273 A CN103690273 A CN 103690273A
- Authority
- CN
- China
- Prior art keywords
- bone
- port
- transfusion port
- biological reactor
- catheter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to an in vivo bioreactor which comprises a catheter. The outlet end of the catheter is arranged in a hollow cavity of an artificial bone material; the tail end of the catheter is fixed on the artificial bone material by a kirschner wire; the periphery of a bone defect position is provided with a fixing net for fixing; the inlet end of the catheter is connected with an outlet of a port-cath; an inlet of the port-cath is connected with a medical constant-flow-rate airbag power pump; plasma in the medical constant-flow-rate airbag power pump is supplied to a body by the catheter through the port-cath; the in vivo bioreactor solves the difficult problems of nutrition supply and blood vessel ingrowth in a material cell compound object, provides an excellent device for treatment of the segmental bone defect and has the characteristics of simple structure and good effect.
Description
Technical field
The invention belongs to field of medical technology, be specifically related to a kind of in-vivo biological reactor, be applicable to the damaged reconstruction of long segment bone that a variety of causes causes, make the biological healing of large segmental bone defect become possibility.
Background technology
Wound, infection and bone tumour resection often cause serious osseous tissue damaged, and particularly large section (more than 20 millimeters) bone is damaged, greatly increase treatment difficulty, cause and occur the series of problems such as disability rate is high, disability time is long, anaphase is difficult.The reparation of large segmental bone defect and reconstruction are a difficult problem and the study hotspots of orthopaedics always.Allograph bone and the band blood vessel autologous bone transplantings of adopting are treated large segmental bone defect more clinically at present, but allograph bone treatment large segmental bone defect also exists immunological rejection and pathophorous danger.Large section of band blood vessel autologous bone transplanting reparation is damaged, is being subject to, for Qu Junyao, is carrying out complicated operation, makes patient bear heavy operation burden, moreover, autologous limited after all for bone amount.Bone tissue engineer technology develop rapidly in recent years, tissue-engineered bone provides comparatively ideal repair mode for bone is damaged.Tissue engineered bone is not limited with its source, have more autologous bone photo like or the more excellent features such as biological function, in the damaged reparation of bone, shown unprecedented superiority.
Meanwhile, bioreactor is being brought into play irreplaceable effect in field of tissue engineering technology.Current numerous scholar, from bionics angle, with the interior environment of external biological reactor simulation in-vivo tissue or organ, builds the research work of tissue engineered bone.The methods such as the rotation by culturing room, center tube perfusion evenly scatter to three-dimensional stent material inside by nutrient substance, the nutrient substance that sustainable supply cell is required.Solved to a certain extent the nutrition supply of material internal.But current external biological reactor and actual internal milieu still exist bigger difference, can not provide the interior microenvironment of body completely, the vascularization problem of unresolved tissue engineered bone.Therefore it can only maintain the survival of material internal cell in vitro, when material is implanted into the damaged place of body internal skeleton, because blood vessel is grown into and is needed time several weeks even a couple of days, cell tolerance anoxia is only a few hours, the nutrition supply of early stage cell only depends on the supply of body tissue liquid, and the extension of oxygen is confined in 200 μ m.The cell of final material internal still can not be survived.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of in-vivo biological reactor, solved the difficult problem that nutrition supply in material cell complexes body and blood vessel are grown into, for the treatment of large segmental bone defect provides good device, there is simple in structure, effective feature.
To achieve these goals, the technical solution used in the present invention is: a kind of in-vivo biological reactor, include conduit, the port of export of conduit is located in the cavity of artificial bone, catheter tip is fixed on artificial bone with Kirschner wire, outside the damaged place of bone, be arranged with for fixing fixed network, duct entry end is connected with the outlet of transfusion port, and transfusion entrance of harbour is connected with medical constant flow rate air bag kinetic pump.
Described transfusion port is connected with transfusion port seat, and transfusion port is by culturing in vivo and two system partitionings of external counterpulsation.Adopt transfusion port to separate, greatly reduced the probability of wound contamination.
Described fixed network adopts titanium net.
Described fixed network is comprised of two semicircular nets, and two semicircular nets are screwed.
Described artificial bone adopts the porous bio-ceramic material of cecum hollow.
The invention has the beneficial effects as follows:
In medical constant flow rate air bag kinetic pump, pack artificial plasm into, blood plasma contains the required various nutrient substance of cells survival and relevant cytokine, is conducive to vascularization and the skeletonization of cell material complex.
Being connected by a tiny Kirschner wire of conduit and artificial bone fixes.Cell material complex is transplanted to damaged place to the marrow, fixing or the outer fixing two ends at the damaged place of bone are fixed in utilizing, artificial bone is enclosed one deck thin layers of titanium net around, prevents that material displacement from dropping, utilize each two screws up and down titanium net to be fixed on to the two ends at the damaged place of bone
The invention provides artificial bone completely internal milieu, guarantee that material implant internal skeleton still can continue to provide nutrition and can impel material vascularization and skeletonization for material internal cell after damaged.Overcoming on the basis of prior art defect, for the treatment of large segmental bone defect clinically provides new technological means.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention.
Fig. 2 is the artificial bone material structure chart of the present invention.
Fig. 3 is the structural representation of fixed network of the present invention.
The specific embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Referring to Fig. 1,2,3, a kind of in-vivo biological reactor, include conduit 1, the port of export of conduit 1 is located in the cavity 5 of artificial bone 4, catheter tip is fixed on artificial bone 4 with a gram assorted pin 3, outside the damaged place of bone, be arranged with for fixing fixed network 6, conduit 1 arrival end is connected with the outlet of transfusion port 2, and the entrance of transfusion port 2 is connected with medical constant flow rate air bag kinetic pump 8.
Described transfusion port is connected with transfusion port seat, and transfusion port is by culturing in vivo and two system partitionings of external counterpulsation.Adopt transfusion port to separate, greatly reduced the probability of wound contamination.
Described fixed network 6 is comprised of two semicircular nets, and two semicircular nets are fixing with screw 7.
Described fixed network 6 adopts titanium net.
Described artificial bone adopts the porous bio-ceramic material of cecum hollow.
The through medullary cavity of a circular aperture is bored at proximal part distance damaged 2cm place at the damaged place of bone.The catheter tip that connects transfusion port is penetrated to this aperture, and extend to the damaged place of bone by medullary cavity, subsequently catheter tip is inserted in the hollow structure of artificial bone, being connected by a tiny Kirschner wire of the duct wall of conduit and artificial bone fixes.Cell material complex is transplanted to damaged place to the marrow, in utilizing, fix or fix the damaged two ends of bone are fixed outward, material is enclosed one deck thin layers of titanium net (3D printing technique around, mesh size is the square of 0.5cmX0.5cm), prevent that material displacement from dropping, utilize each two screws up and down titanium net to be fixed on to the two ends at the damaged place of bone, now cell material complex is wrapped up by soft tissues such as muscle around.The other end of transfusion port conduit is connected with transfusion port seat, is fixed under skin.After cleaning a wound, successively close wound, skin suture, only tangible to a circular protrusion at body surface, by a not damaged pin percutaneous vertical puncturing in the reservoir of transfusion port injection seat.This not damaged pin is connected with a medical constant flow rate air bag kinetic pump.This whole set of structure constituted in-vivo biological reactor.This type bioreactor not only provides large section of tissue-engineered bone nutrition supply in vivo, and the in-house blood vessel of infantile myasthenia around is constantly inwardly grown into.Make material from inside and outside two aspects simultaneously skeletonization with become blood vessel, accelerated the healing that cell material complex and two ends bone are damaged.
Operation principle of the present invention:
Blood plasma process blood transfusion port in medical constant flow rate air bag kinetic pump is by supplying with in conduit donor.
1) in medical constant flow rate air bag kinetic pump, pack artificial plasm into, blood plasma contains the required various nutrient substance of cells survival and relevant cytokine, is conducive to vascularization and the skeletonization of cell material complex.
2) strict flow and flow velocity while controlling perfusion is 2.0ml/h, due to the artificial plasm of perfusion flows through can not be completely by Cell uptake after the artificial bone of porous, therefore unnecessary blood plasma will be absorbed by infantile myasthenia tissue around.Research finds that surrounding soft tissue's average crystalline liquid infiltration rate is 0.125ml/ (hcm
2).
3) Continuous Perfusion is two weeks, and research finds to be wrapped in simple artificial bone blood vessel in two weeks in soft tissue material deep of having grown into.Therefore, pour into after two weeks a large amount of blood vessels material internal of having grown into, being enough to provides nutrient substance for remaining cell.
4) after two weeks, stop perfusion, and transfusion port, Kirschner wire and thin layers of titanium net are removed completely.
In-vivo biological reactor of the present invention can overcome the defect of prior art completely, guarantee artificial bone inner and outside two aspects vascularization simultaneously and skeletonization, not only the treatment for large segmental bone defect provides new technological means, also for clinical treatment has been created new mode of thinking.
Claims (5)
1. an in-vivo biological reactor, include conduit (1), it is characterized in that, the port of export of conduit (1) is located in the cavity (5) of artificial bone (4), Kirschner wire for catheter tip (3) is fixed on artificial bone (4), outside the damaged place of bone, be arranged with for fixing fixed network (6), conduit (1) arrival end is connected with the outlet of transfusion port (2), and the entrance of transfusion port (2) is connected with medical constant flow rate air bag kinetic pump (8).
2. a kind of in-vivo biological reactor according to claim 1, is characterized in that, described transfusion port is connected with transfusion port seat, and transfusion port is by culturing in vivo and two system partitionings of external counterpulsation.
3. a kind of in-vivo biological reactor according to claim 1, is characterized in that, described fixed network (6) is comprised of two semicircular nets, two semicircular for net screw (7) fixing.
4. a kind of in-vivo biological reactor according to claim 1, is characterized in that, described fixed network (6) adopts titanium net.
5. a kind of in-vivo biological reactor according to claim 1, is characterized in that, described artificial bone adopts the porous bio-ceramic material of cecum hollow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310586372.2A CN103690273A (en) | 2013-11-21 | 2013-11-21 | In vivo bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310586372.2A CN103690273A (en) | 2013-11-21 | 2013-11-21 | In vivo bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103690273A true CN103690273A (en) | 2014-04-02 |
Family
ID=50352053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310586372.2A Pending CN103690273A (en) | 2013-11-21 | 2013-11-21 | In vivo bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103690273A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104188738A (en) * | 2014-08-14 | 2014-12-10 | 卢建熙 | Multi-functional internal bone generator |
| CN105597188A (en) * | 2016-01-29 | 2016-05-25 | 江苏义倍医疗科技股份有限公司 | Medical infusion device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201648A (en) * | 1997-06-09 | 1998-12-16 | 山东省文登整骨医院 | Genesis body of bone and joint |
| CN1346403A (en) * | 1999-02-04 | 2002-04-24 | 技术研究及发展基金有限公司 | Method and apparatus or maintenance and expansion of hemopoietic stem cells and/or progenitor cells |
| CN1688267A (en) * | 2002-08-20 | 2005-10-26 | 精密技术公司 | Composition for the carrying and delivery of bone growth inducing material and methods for producing and applying the composition |
| CN1905921A (en) * | 2003-11-26 | 2007-01-31 | 德普伊斯派尔公司 | Local intraosseous administration of bone forming agents and anti-resorptive agents and devices therefor |
| CN1974750A (en) * | 2006-11-16 | 2007-06-06 | 华东理工大学 | Two-phase bioreactor system for extracorporeal construction of histoengineering cartilage |
| CN101091804A (en) * | 2007-07-18 | 2007-12-26 | 戴江华 | Method for constructing tissue-engineering bone by pouring stem cell of inducing bone marrow ground substance into vivo |
| CN202136446U (en) * | 2011-07-06 | 2012-02-08 | 中国人民解放军第四军医大学 | Assorted circulatory perfusing inoculation loop for external building of activated artificial bone |
| CN203564380U (en) * | 2013-11-21 | 2014-04-30 | 中国人民解放军第四军医大学 | Internal bioreactor |
-
2013
- 2013-11-21 CN CN201310586372.2A patent/CN103690273A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201648A (en) * | 1997-06-09 | 1998-12-16 | 山东省文登整骨医院 | Genesis body of bone and joint |
| CN1346403A (en) * | 1999-02-04 | 2002-04-24 | 技术研究及发展基金有限公司 | Method and apparatus or maintenance and expansion of hemopoietic stem cells and/or progenitor cells |
| CN1688267A (en) * | 2002-08-20 | 2005-10-26 | 精密技术公司 | Composition for the carrying and delivery of bone growth inducing material and methods for producing and applying the composition |
| CN1905921A (en) * | 2003-11-26 | 2007-01-31 | 德普伊斯派尔公司 | Local intraosseous administration of bone forming agents and anti-resorptive agents and devices therefor |
| CN1974750A (en) * | 2006-11-16 | 2007-06-06 | 华东理工大学 | Two-phase bioreactor system for extracorporeal construction of histoengineering cartilage |
| CN101091804A (en) * | 2007-07-18 | 2007-12-26 | 戴江华 | Method for constructing tissue-engineering bone by pouring stem cell of inducing bone marrow ground substance into vivo |
| CN202136446U (en) * | 2011-07-06 | 2012-02-08 | 中国人民解放军第四军医大学 | Assorted circulatory perfusing inoculation loop for external building of activated artificial bone |
| CN203564380U (en) * | 2013-11-21 | 2014-04-30 | 中国人民解放军第四军医大学 | Internal bioreactor |
Non-Patent Citations (2)
| Title |
|---|
| 李祥 等: "旋转灌注式生物反应器系统构建及在骨组织工程中的应用", 《生物医学工程学杂志》, vol. 24, no. 1, 31 December 2007 (2007-12-31), pages 66 - 70 * |
| 谢幼专 等: "柱状多孔磷酸三钙载体内细胞增殖与流场变化的研究", 《医用生物力学》, vol. 23, no. 1, 29 February 2008 (2008-02-29), pages 52 - 56 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104188738A (en) * | 2014-08-14 | 2014-12-10 | 卢建熙 | Multi-functional internal bone generator |
| CN104188738B (en) * | 2014-08-14 | 2018-04-27 | 卢建熙 | Multifunctional body in-seam generator |
| CN105597188A (en) * | 2016-01-29 | 2016-05-25 | 江苏义倍医疗科技股份有限公司 | Medical infusion device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105586249B (en) | Circulating perfusion bioreactor device capable of realizing circulating perfusion of three-dimensional support | |
| US8968401B2 (en) | Synthetic scaffolds and organ and tissue transplantation | |
| ES2546734T3 (en) | Rapid preparation and use of tissues and structures obtained by tissue engineering as individual implants | |
| ES2620957T3 (en) | Liporrellene with stem cells derived from adipose tissue expanded ex vivo for cosmetic breast filling or for facial filling and / or rejuvenation | |
| US11773362B2 (en) | Modular bioreactor, compliance chamber for a bioreactor, and cell seeding apparatus | |
| CN101492655A (en) | Vascularized fat depot based on partition and construction method thereof | |
| CN102114275B (en) | Hepatic lobule-like bioreactor | |
| CN203564380U (en) | Internal bioreactor | |
| CN101259292A (en) | Construction method of tissue engineering blood vessel | |
| WO2017152343A1 (en) | Recirculating perfusion bioreactor device that can realize three-dimensional scaffold recirculating perfusion | |
| CN103690273A (en) | In vivo bioreactor | |
| CN101993853A (en) | Injection type vascularized adipose tissue and construction method thereof | |
| CN108624581A (en) | A kind of microballoon and brainpower insufflation system of mescenchymal stem cell materials for binding biological | |
| WO2011150726A1 (en) | Stem cell filtering screening enrichment and quickly compounding device | |
| Visconti et al. | Cardiovascular tissue engineering I. Perfusion bioreactors: a review | |
| CN108042567A (en) | For the life assemblage preparation of repair of cartilage and its application | |
| CN210932936U (en) | Tissue engineering bone | |
| Sterodimas | Tissue engineering with adipose derived stem cells (ADSCs) in plastic & reconstructive surgery: current and future applications | |
| CN103160433A (en) | Rotary barrel type tissue culture device | |
| CN108379663B (en) | Device and method for trapping and enriching cells in porous regular body scaffold material | |
| CN104707181B (en) | A kind of intervention microcirculqtory system and its operational approach | |
| CN204798437U (en) | Intervene little circulation system | |
| CN114480127B (en) | Regeneration reaction chamber for in-situ repair of large-section load bone defects and application method thereof | |
| WO2018132071A1 (en) | Method and apparatus for in-vitro tissue cultivation | |
| RU2513593C2 (en) | Bioartificial system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140402 |