CN103690262A - Electrode adapter for avian chronic brain function researches and fixing method - Google Patents
Electrode adapter for avian chronic brain function researches and fixing method Download PDFInfo
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- CN103690262A CN103690262A CN201310620549.6A CN201310620549A CN103690262A CN 103690262 A CN103690262 A CN 103690262A CN 201310620549 A CN201310620549 A CN 201310620549A CN 103690262 A CN103690262 A CN 103690262A
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Abstract
The invention relates to an electrode adapter for avian chronic brain function researches and a fixing method, which belong to the field of neurobiology. According to the features of an avian skull, screw-fixing holes, electrode implantation holes and pits are drilled at specific points on the avian skull; electrodes are sequentially implanted into the electrode implantation holes and then fixed, so that a primary substrate is formed; the electrode adapter is placed upon the primary substrate and fixed; electrode leads are correspondingly connected with electrode-adapting holes and fixed. The electrode adapter comprises a substrate and a bus bar, the substrate is provided with the electrode-adapting holes, the electrode-adapting holes are connected with the bus bar, and the bus bar is provided with bus bar interfaces, the number of which corresponds to the number of the electrode-adapting holes. The invention realizes the dual fixation of the electrode adapter deep in the avian skull and on the surface layer of the avian skull, the electrode adapter is applicable to the overall research of the discretely distributed areas in the avian brain, the stationary time of the electrode adapter is remarkably prolonged, and therefore the electrode adapter is favorable for the implementation of long-term electroencephalographic research and the chronic experimental researches of foreign body-implanted brain function change and the like.
Description
Technical field
The present invention relates to electrode switching device and fixing means for a kind of birds chronic cerebral functional study, belonged to neurobiology field.
Background technology
From 1791, Italian biologist Galvani found bio-electric phenomenon existing more than 200 year history so far.Potential change in nerve conduction process in 1848 is measured first.Hess has realized the research to sobering animal Different brain region function by the method for implanted electrode in brain.In this hindbrain, the method for implanted electrode is widely used in the brain zone function research under animal waking state, such as: after the midbrain of the little brain function research of waking state bull frog, electricity irritation cat, hypothalamus, bring out the research of act of defence and green iguana Different brain region function etc.
For the research modes that adopt wired stimulation of free waking state animal brain zone function, limited to a certain extent the research of animal movement behavior in early days more.Along with developing rapidly of the technology such as microelectronics, retrofit, communication, the wireless stimulating apparatus that meets different demands is come out one after another, and this provides technical support for the brain zone function of more in depth zoologizeing under free state.Current electrode switching device is mainly used in the bio electricity collection of mammal etc., and electrode to implant some position relatively fixing, cannot realize the chronic experiment research of birds Different brain region.In addition, birds have increased electrode switching device and the skull difficulty fixedly time for the skeleton specialization (loose porous) that adapts to flying activity and occur during evolution, be unfavorable for the long-term fixing of electrode switching device, having been reported is only about 2 months, therefore cannot carry out long-term experiment research to birds Different brain region chronic functional.
Summary of the invention
The invention reside in electrode switching device and fixing means for a kind of birds chronic cerebral functional study is provided, to solve the technical problem that in existing research, the electrode switching device set time is short, electrode implantation site distribution is little.
In order to solve the problems of the technologies described above, the fixing means of electrode switching device for a kind of birds chronic cerebral functional study provided by the invention, is characterized in that comprising the following steps:
1), birds are anaesthetized after, the skull of surgical exposure birds brain district top;
2), above the midbrain of the birds left and right sides, before skull and fontanel point, distinguish drilling screw fixing hole; According to definite electrode, implant some position and on the skull above birds object brain district, bore electrode implantation hole, and at described electrode, implant hole skull surface around and bore pit;
3),, hold-down screw is screwed into respectively to step 2) before described birds left and right sides midbrain top skull and fontanel point in screw fixed hole, then tinsel is wound on the hold-down screw above the midbrain of the birds left and right sides and uses with reference to the utmost point as stimulation;
4), successively in step 2) electrode of described top, birds object brain district implants hole and is implanted into electrode fixing;
5), in step 2) skull surface of described top, birds object brain district smears medical adhesive gel material to step 4) institute's implant electrode carries out integral body and fixes, after solidifying, form elementary base plate, described elementary base plate is simultaneously to step 2) the described electrode pit of implanting surrounding cranial bone surface, hole fills, and forms contact cone;
6), electrode switching device is placed in to step 2) described top, birds object brain district, making step 3) described hold-down screw is positioned at the limited impression of described electrode switching device, with medical adhesive gel material, the gap between described electrode switching device and hold-down screw is filled, realize fixing between described electrode switching device and hold-down screw;
7), with medical adhesive gel material in step 6) the base plate side of described electrode switching device is coated, and in base plate and step 5) fill between described elementary base plate, realize fixing with described elementary base plate;
8), electrode leads to client correspondence is connected to step 6) in the electrode switching hole of described electrode switching device and fix.
In the present invention, described step 1) the birds anaesthesia process in is: after with 2% pentobarbital sodium solution 1.5ml/kg chest muscle intramuscular injection, birds being anaesthetized sb. generally, then containing the lidocaine hydrochloride of epinephrine inj 200,000/concentration, carry out local anesthesia at birds field of operation subcutaneous injection 0.5ml.
In the present invention, described step 2) screw fixed hole in and electrode are implanted hole and are adopted the flexible cranial drill being installed on the three-dimensional transfer arm of brain solid positioner, with rose drill, drill through, and the degree of depth that described electrode is implanted hole just penetrates skull; Described pit adopts cranial drill to implant hole at electrode and around with 1mm spacing, in skull surface, drills through.
In the present invention, described step 4) electrode implantation process is:
1), open bipolar programmable stimulato, regulate stimulus parameter, connect stimulation circuit, electrode is installed in brain position finder electrode adapter, then move to birds muscle of head contact with it, send to stimulate and determine the loop conducting situation that stimulates;
2), determine and stimulate after loop conducting, through electrode, implant hole feeder electrode, near limit stimulation limit feeding in the time of arrival object brain district, determines and arrives feed-disabling behind object brain district, with medical EC glue, fill the gap between electrode and electrode implantation hole, then use medical adhesive gel material fixed electrode;
3), successively at other different Nao district implant electrodes fixing.
In the present invention, it is the nichrome wire of 100 μ m, Teflon insulation that described electrode adopts diameter.
In the present invention, described step 6) electrode switching device manufacture method is:
1), determine the size of electrode switching device;
2), according to definite number of electrodes, draw switching device layout and also make base plate;
3) row that, the exit of processing and base plate matches is female;
4), mother row after processing is welded in to exit on base plate the female pin of worn row at the ledge of base plate bottom surface;
5), base plate is polished into the terrace with edge structure that upper surface is slightly less than lower surface.
The present invention also provides a kind of birds chronic cerebral functional study electrode switching device, it is characterized in that: described electrode switching device comprises that base plate is female with the row who is connected with base plate exit, described base plate is provided with some electrode switchings hole, electrode switching hole connecting bottom board exit, and be communicated with row is female by base plate exit, on row mother, be provided with the row maternal interface corresponding with electrode switching hole.
In the present invention, described base plate is the terrace with edge structure that upper surface is less than lower surface.
In the present invention, a relative side of arranging maternal interface on described base plate is provided with limited impression.
In the present invention, described limited impression is 2.
Beneficial effect of the present invention is:
(1), fixing means provided by the invention taken into full account birds skeleton characteristic, realized the dual fixing of birds skull deep layer and skull top layer and electrode switching device, extended electrode switching device service life;
(2), the present invention is by the switching to implant electrode exit, realized the holistic approach to Discrete Distribution brain district in birds brain;
(3), the present invention uses electrode switching device volume little, lightweight, is applicable to the fixing of all kinds of birds heads, on birds normal activity, can not distinguish impact;
(4), easy to use, simple to operate, the good stability of the present invention.
Accompanying drawing explanation
In Fig. 1 the present invention, electrode switching device connects the schematic top plan view after implant electrode exit whole fixing.
Electrode switching device structural representation in Fig. 2 the present invention.
In Fig. 3 the present invention, electrode switching device connects the coronalplane schematic diagram after implant electrode exit whole fixing.
In figure, 1-skull, 2-hold-down screw, the elementary base plate of 3-, 4-arrange female with reference to the utmost point, 5-base plate, 6-electrode leads to client, 7-dentistry cement, 8-row maternal interface, 9-limited impression, 10-electrode switching hole, 11-contact cone, 12-electrode, 13-cerebral tissue, 14-.
The specific embodiment
To the present invention-birds chronic cerebral, functional study describes in detail with the fixing means of electrode switching device as example below in conjunction with accompanying drawing, to take pigeon, and this method comprises the following steps:
1), electrode switching device manufacturing process: as shown in Figure 1, 2, determine pigeon skull shape characteristic, according to the required number of electrodes of brain function research, draw electrode switching device layout, make base plate 5; Mother row 14 that the exit of processing and base plate 5 matches; Mother row 14 after processing is welded in to electrode switching device exit, and the row who removes on the pricking with needle electrode insertion switching device of abutting end needle body is female.With the female pin of fine sandpaper polishing row at the ledge of wiring board bottom surface to reduce electrode switching device height, reduce the volume of electrode switching device; , polished in the side of base plate 5 meanwhile, make base plate form the terrace with edge spline structure that a upper surface is slightly less than lower surface, so that fixing better.In the present embodiment, base plate 5 adopts pcb board, and row female 14 comprises 9 row's maternal interfaces, overall dimensions: long 12.8mm, wide 9.5mm, high 5.5mm, weight is 0.42g.
2) pigeon anesthesia and head are fixed: with medical alcohol, the nearly xiphoid-process side of pigeon chest muscle is carried out to skin degerming, expose breast flesh, use 1ml syringe from pigeon chest muscle middle part, xiphoid-process side is opened 1.5-2.0cm place parallel xiphoid-process direction inserting needle and is injected 2% pentobarbital sodium solution by 1.5ml/kg, carry out pigeon general anesthesia, until pigeon health, lie prone after sleeping nose drop, with the moistening pigeon calvarium of cotton ball soaked in alcohol feather, medical iodophor disinfection, then at field of operation subcutaneous injection 0.5ml, containing the lidocaine hydrochloride of epinephrine inj 200,000/concentration, carry out local anesthesia, to improve analgesia effect.After anesthesia, pigeon head is fixed on the three-dimensional locating and clamping apparatus of bird brain.
3) calvarium skin incision: fully soak pigeon calvarium feather with normal saline, Shearing shears is wiped out field of operation top feather, medical povidone iodine opponent visual area skin carries out disinfection, after determining field of operation scope, mosquito forceps clamps skin 5 min left and right, folder closes little blood vessel wherein, and the tissue shear disinfecting in alcohol is wiped out side skin on operating field, and carries out in time hemostasis and process, sterilized cotton ball is wiped skull surface connective tissue, exposes skull 1.
4) sphenotresia and roughening are processed: flexible cranial drill is installed on the three-dimensional transfer arm of brain solid positioner, with φ 0.5mm rose drill drilling screw fixing hole before skull (after fontanel point 5.0mm, sagittal suture each side 4.0mm) and fontanel point above the midbrain of the pigeon left and right sides, according to definite electrode, implant some position and on the skull above object brain district, drill through electrode implantation hole, the degree of depth just penetrates skull; With cranial drill, at electrode, implant hole and with 1mm spacing, in skull surface, bore pit around, carry out roughening processing.
5) be screwed into hold-down screw, be wound around with reference to the utmost point: the hold-down screw 2 that tip is carried out to Passivation Treatment is screwed in pigeon left and right sides midbrain top skull and the front screw fixed hole of fontanel point, be screwed into degree of depth 3mm left and right, then the hold-down screw 2 that filamentary silver is wound in to pigeon left and right sides midbrain top is as with reference to the utmost point 4, as shown in Figure 1.In the present embodiment, hold-down screw 2 is M0.8*5.0mm, fixing hole φ 0.6mm.Above-mentioned filamentary silver also can adopt other tinsels to replace.
6) electrode is implanted: as shown in Figure 3, open bipolar programmable stimulato, regulate stimulus parameter, connect stimulation circuit, electrode 12 is installed on to position finder electrode adapter, move to pigeon muscle of head contact with it, send and stimulate observation pigeon muscle to have atremia, determine the loop conducting situation that stimulates.Stimulate after loop conducting, from electrode, implant hole feeder electrode 12, near limit stimulation limit feeding in the time of arrival object brain district, determine feed-disabling behind object brain district, with medical EC glue, fill the gap between electrode 12 and electrode implantation hole 10, then dentistry cement fixed electrode, implants and fixed electrode at other object cerebral tissue 13 successively.In the present embodiment, electrode is the nichrome wire of diameter 100 μ m, Teflon insulation.
7) the elementary base plate of the fixing formation of electrode: as shown in Figure 3, the thin layer of coating a thickness 1mm left and right with the dentistry cement of thickness on skull 1 surface carries out integral body to electrode 12 to be fixed, after solidifying, it forms elementary base plate 3, elementary base plate 3 is processed to skull 1 roughening the pit forming simultaneously and is filled, and forms contact cone 11 after solidifying.
8) electrode switching device is fixed and electrode leads to client connection: as shown in Figure 1,3, electrode switching device is placed in to skull 1 top, make hold-down screw 2 be positioned at the limited impression 9 of electrode switching device, with dentistry cement, the gap of 2 of electrode switching device and hold-down screws is filled, realize the fixing of 2 of described electrode switching device and hold-down screws; Meanwhile, base plate 5 sides with dentistry cement at electrode switching device are coated, and fill between base plate and above-mentioned elementary base plate 3, electrode switching device and elementary base plate 3 are fully contacted fixing; Then electrode leads to client 6 correspondences are connected in the electrode switching hole 10 on electrode switching device, with scolding tin, weld, with the whole base plate 5 that covers of dentistry cement 7, realize the integral body of electrode switching device and electrode 12 and fix.
9) post surgery treatment: etc. dentistry cement 7 surgical wound is carried out to iodophor disinfection after solidifying, and smear gentamycin sulfate and protect from infection.Pigeon is taken off from brain solid positioner, feed a small amount of light salt brine, carry out postoperative insulation and raise and recover separately.
Above-mentioned dentistry cement 7 also can adopt other medical adhesive gel materials.
As shown in Figure 2, electrode switching device in the present embodiment comprises base plate 5 and mother row 14 who welds mutually with base plate 5 exits, on base plate 5, be provided with 9 electrode switching holes 10, electrode switching hole 10 connecting bottom board 5 exits, and be connected with row female 14 by base plate 5 exits, the row maternal interface 8 corresponding with electrode switching hole 10 is set on row female 14, and row's maternal interface 8 is communicated with electrode switching hole 10 through base plate 5 exits.Base plate 5 adopts pcb boards to make, and a relative side of arranging maternal interface 8 on base plate 5 is provided with 2 limited impressions 9, for and hold-down screw 2 between fixing; Base plate 5 is less than the terrace with edge structure of lower surface for upper surface, so that base plate 5 and above-mentioned elementary base plate 3 is fixing.
The quantity in the switching hole 10 in above-mentioned electrode switching device can increase and decrease according to actual implant electrode quantity in research process, and row's maternal interface 8 keeps corresponding with switching hole 10; Limited impression 9 quantity also can need to increase and decrease according to reality is fixing.
The chronic brain stimulation research that electrode switching device of the present invention and fixing means also can be used for other birds, can realize the research of pigeon and other birds Different brain region neuron electric discharge equally by changing implant electrode.
The above embodiment has only expressed one embodiment of the present invention, and its description can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some improvement, these all should fall into protection scope of the present invention.
Claims (10)
1. a fixing means for electrode switching device for the functional study of birds chronic cerebral, is characterized in that comprising the following steps:
1), birds are anaesthetized after, the skull (1) of surgical exposure birds brain district top;
2), above the midbrain of the birds left and right sides, before skull and fontanel point, distinguish drilling screw fixing hole; According to definite electrode, implant some position and on the skull above birds object brain district, bore electrode implantation hole, and at described electrode, implant hole skull surface around and bore pit;
3),, hold-down screw (2) is screwed into respectively to step 2) before described birds left and right sides midbrain top skull and fontanel point in screw fixed hole, the hold-down screw (2) that then tinsel is wound in to midbrain top, the birds left and right sides is above used with reference to the utmost point (4) as stimulation;
4), successively in step 2) electrode of described top, birds object brain district implants hole and is implanted into electrode (12) fixing;
5), in step 2) skull surface of described top, birds object brain district smears medical adhesive gel material to step 4) institute's implant electrode (12) carries out integral body and fixes, after solidifying, form elementary base plate (3), described elementary base plate (3) is simultaneously to step 2) the described electrode pit of implanting surrounding cranial bone surface, hole fills, and forms contact cone (11);
6), electrode switching device is placed in to step 2) described top, birds object brain district, making step 3) described hold-down screw (2) is positioned at the limited impression (9) of described electrode switching device, with medical adhesive gel material, the gap between described electrode switching device and hold-down screw (2) is filled, realize fixing between described electrode switching device and hold-down screw;
7), with medical adhesive gel material in step 6) base plate (5) side of described electrode switching device is coated, and in base plate (5) and step 5) fill between described elementary base plate (3), realize fixing with described elementary base plate (3);
8), electrode leads to client (6) correspondence is connected to step 6) in the electrode switching hole (10) of described electrode switching device and fixing.
2. the fixing means of electrode switching device for birds chronic cerebral functional study according to claim 1, it is characterized in that described step 1) in birds anaesthesia process be: after with 2% pentobarbital sodium solution 1.5ml/kg chest muscle intramuscular injection, birds being anaesthetized sb. generally, then containing the lidocaine hydrochloride of epinephrine inj 200,000/concentration, carry out local anesthesia at birds field of operation subcutaneous injection 0.5ml.
3. the fixing means of electrode switching device for birds chronic cerebral functional study according to claim 1, it is characterized in that described step 2) in screw fixed hole and electrode implant hole and adopt the flexible cranial drill being installed on the three-dimensional transfer arm of brain solid positioner, with rose drill, drill through, the degree of depth that described electrode is implanted hole just penetrates skull; Described pit adopts cranial drill to implant hole at electrode and around with 1mm spacing, in skull surface, drills through.
4. the fixing means of electrode switching device for birds chronic cerebral functional study according to claim 1, is characterized in that described step 4) electrode implantation process be:
1), open bipolar programmable stimulato, regulate stimulus parameter, connect stimulation circuit, electrode (12) is installed in brain position finder electrode adapter, then move to birds muscle of head contact with it, send to stimulate and determine the loop conducting situation that stimulates;
2), determine and stimulate after loop conducting, through electrode, implant hole feeder electrode (12), near limit stimulation limit feeding in the time of arrival object brain district, determine and arrive feed-disabling behind object brain district, with medical EC glue, fill the gap between electrode and electrode implantation hole, then use medical adhesive gel material fixed electrode (12);
3), successively at other different Nao district (13) implant electrodes (12) fixing.
5. the fixing means of electrode switching device for birds chronic cerebral functional study according to claim 1, is characterized in that: it is the nichrome wire of 100 μ m, Teflon insulation that described electrode (12) adopts diameter.
6. the fixing means of electrode switching device for birds chronic cerebral functional study according to claim 1, is characterized in that described step 6) electrode switching device manufacture method be:
1), determine the size of electrode switching device;
2), according to definite number of electrodes, draw switching device layout and also make base plate (5);
3) row female (14) that, the exit of processing and base plate (5) matches;
4), the row after processing female (14) is welded in to exit on base plate (5) female (14) pin of worn row at the ledge of base plate (5) bottom surface;
5), base plate (5) is polished into the terrace with edge structure that upper surface is slightly less than lower surface.
7. according to the birds chronic cerebral functional study electrode switching device described in claim 1-6 any one, it is characterized in that: described electrode switching device comprises base plate (5) and the row who is connected with base plate (5) exit female (14), described base plate (5) is provided with some electrode switchings hole (10), electrode switching hole (10) connecting bottom board (5) exit, and be communicated with row female (14) by base plate (5) exit, on row female (14), be provided with the row maternal interface (8) corresponding with electrode switching hole (10).
8. birds chronic cerebral according to claim 7 functional study electrode switching device, is characterized in that: described base plate (5) is less than the terrace with edge structure of lower surface for upper surface.
9. birds chronic cerebral according to claim 8 functional study electrode switching device, is characterized in that: a relative side of described base plate (5) upper row maternal interface (8) is provided with limited impression (9).
10. birds chronic cerebral according to claim 9 functional study electrode switching device, is characterized in that: described limited impression (9) is 2.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104199446A (en) * | 2014-09-18 | 2014-12-10 | 山东科技大学 | Robot bird flying controllability evaluation system and evaluation method |
| CN108742942A (en) * | 2018-06-19 | 2018-11-06 | 中国科学院电子学研究所 | General anesthesia neuromechanism studies acute experiment platform and chronic experiment platform |
| CN110876616A (en) * | 2019-12-11 | 2020-03-13 | 中国科学院深圳先进技术研究院 | Self-extending recording electrode and preparation method and implantation method thereof |
| CN113080981A (en) * | 2021-03-26 | 2021-07-09 | 燕山大学 | Animal robot brain electrode switching device |
| CN114190948A (en) * | 2021-12-14 | 2022-03-18 | 深圳先进技术研究院 | Manufacturing method and using method of multi-brain-area recording electrode array capable of being implanted for long time |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050159670A1 (en) * | 2003-12-18 | 2005-07-21 | Sneddon & Associates Inc. | Method for imaging information, change in information, and computation in the brain |
| CN201142341Y (en) * | 2007-12-21 | 2008-10-29 | 赵建和 | Electrode switching plate of series battery group |
| CN101999897A (en) * | 2010-11-09 | 2011-04-06 | 南京航空航天大学 | Microelectrode propelling device and method |
| CN102784010A (en) * | 2012-08-16 | 2012-11-21 | 杭州电子科技大学 | Rat primary auditory cortex microelectrode array implantation fixation operation method |
| CN103190894A (en) * | 2012-01-04 | 2013-07-10 | 旺三丰兴业股份有限公司 | Improved device for collecting physiological signals |
-
2013
- 2013-11-29 CN CN201310620549.6A patent/CN103690262B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050159670A1 (en) * | 2003-12-18 | 2005-07-21 | Sneddon & Associates Inc. | Method for imaging information, change in information, and computation in the brain |
| CN201142341Y (en) * | 2007-12-21 | 2008-10-29 | 赵建和 | Electrode switching plate of series battery group |
| CN101999897A (en) * | 2010-11-09 | 2011-04-06 | 南京航空航天大学 | Microelectrode propelling device and method |
| CN103190894A (en) * | 2012-01-04 | 2013-07-10 | 旺三丰兴业股份有限公司 | Improved device for collecting physiological signals |
| CN102784010A (en) * | 2012-08-16 | 2012-11-21 | 杭州电子科技大学 | Rat primary auditory cortex microelectrode array implantation fixation operation method |
Non-Patent Citations (3)
| Title |
|---|
| LONGNIAN LIN等: "《large-scale neural ensemble recording in the brains of freely behaving mice》", 《JOURNAL OF NEUROSCIENCE METHODS》 * |
| SHAOHUA XU等: "《a multi-channel telemetry system for brain microstimulation in freely roaming animals》", 《JOURNAL OF NEUROSEINCE METHODS》 * |
| 刘振东: "《微电极推进器的研制及其在动物运动调控中的运用》", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104199446A (en) * | 2014-09-18 | 2014-12-10 | 山东科技大学 | Robot bird flying controllability evaluation system and evaluation method |
| CN104199446B (en) * | 2014-09-18 | 2017-01-25 | 山东科技大学 | Robot bird flying controllability evaluation system and evaluation method |
| CN108742942A (en) * | 2018-06-19 | 2018-11-06 | 中国科学院电子学研究所 | General anesthesia neuromechanism studies acute experiment platform and chronic experiment platform |
| CN110876616A (en) * | 2019-12-11 | 2020-03-13 | 中国科学院深圳先进技术研究院 | Self-extending recording electrode and preparation method and implantation method thereof |
| CN113080981A (en) * | 2021-03-26 | 2021-07-09 | 燕山大学 | Animal robot brain electrode switching device |
| CN114190948A (en) * | 2021-12-14 | 2022-03-18 | 深圳先进技术研究院 | Manufacturing method and using method of multi-brain-area recording electrode array capable of being implanted for long time |
| WO2023109624A1 (en) * | 2021-12-14 | 2023-06-22 | 深圳先进技术研究院 | Manufacturing method and usage method for recording electrode array capable of being implanted into multiple brain areas for long time |
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