CN103674953B - A kind of method obtaining leather sample cross section sequence of pictures - Google Patents
A kind of method obtaining leather sample cross section sequence of pictures Download PDFInfo
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- CN103674953B CN103674953B CN201310670321.8A CN201310670321A CN103674953B CN 103674953 B CN103674953 B CN 103674953B CN 201310670321 A CN201310670321 A CN 201310670321A CN 103674953 B CN103674953 B CN 103674953B
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Abstract
The present invention relates to a kind of method obtaining leather cross section sequence of pictures, it is characterized in that using epoxy resin and curing agent mixture dipping leather sample, leather fiber is fixed, then it is successively polished, the operation such as polishing, obtain sequence cross section picture under the microscope.Be that sequence of pictures registration is ready by pre-buried mark, by measuring the spacing of the thickness determination sequence of pictures of matrix, in subsequent treatment accurately reflection leather fiber braiding structure lay a solid foundation.
Description
Technical field
The present invention relates to a kind of method obtaining sequence of pictures, be specifically related to a kind of method obtaining leather sample cross section sequence of pictures.
Background technology
Many results of study show: the performance position difference of rawhide and resultant Leather is very large, but the change of the intensity position of fibrous bundle difference is little, and this just illustrates that the existence of position difference is mainly caused by the woven network difference of fibrous bundle.Because before deformation occurs fibrous bundle itself, can first there is comparatively large deformation in network of fibers.As can be seen here, leather fiber Network Weaving mode is very large on Leather Properties impact, but existing network of fibers research method is mainly undertaken by the various sections observing leather tissue, namely the true woven network of leather fiber is inferred by a certain cross section of analytical sample, there is a lot of uncertainties in this deduction, lacks scientific basis.So need to set up leather 3 D weaving network structure by Three Dimensional Reconfiguration, then 3 D weaving network structure is studied.Set up three-dimensionalreconstruction model, first just need to obtain leather cross section sequence of pictures.Existing method is mainly used serial section method traditional in histology and is carried out, but due to leather fiber intensive, the fiber in section is yielding, disappearance serious, and section is difficult to continuously.We are through attempting, and Successful tests goes out a kind of leather sample preparation method (ZL201110179680.4) being applicable to metallography microscope sem observation, but the method is only suitable for observing single cross-sectional image, if want to obtain sequence of pictures, also need other more multi-step.
Summary of the invention
The object of the invention is to overcome above-mentioned prior art not enough, the distortion of leather cross-section fibers is little, continuity strong, sequence of pictures acquisition methods simple to operate to provide one to make.
summary of the invention
Obtain a method for leather sample cross section sequence of pictures, it is characterized in that using epoxy resin and curing agent mixture dipping leather sample, leather fiber is fixed, then it is successively polished, the operation such as polishing, obtain sequence cross section picture under the microscope.Be that sequence of pictures registration is ready by pre-buried mark, by measuring the spacing of the thickness determination sequence of pictures of matrix.
detailed Description Of The Invention
Obtain a method for leather sample cross section sequence of pictures, its step is as follows:
(1) epoxy hardener of 20-30 mass parts is added in 100 parts of epoxy resin stir, be put into and shaking table vibrates 5 minutes discharge the air be mixed into, obtain resin adhesive liquid;
(2) leather sample is immersed in the resin adhesive liquid that step (1) prepares, making leather sample fully suck resin by stirring collision, then taking out sample, put into 60 DEG C of thermostatic drying chambers solidification 2-3 hour, obtain solidifying sample block;
(3) by smooth with sand papering for each of the sample block be cured, and entirely inwardly polished in two less for vertical range opposite A faces and B face (as shown in Figure 1), until expose the leather surface position of needs observation;
(4) the sample block that step (3) has been polished is put in the middle of two exceptionally straight filiforms, filiform position is regulated to make A and the B face central authorities of sample block be close to filiform, then on sample block and filiform, evenly drip the resin adhesive liquid that step (1) prepares, filiform is covered by resin completely, and after waiting its spontaneous curing, filiform is fixed on sample block;
(5) cut off filiform, take off the sample block in step (4), by smooth for the two ends sand papering of sample block, then be vertically fixed in mould, the resin adhesive liquid that implantation step (1) is obtained in mould, at normal temperatures solidification, the demoulding, obtain the solidification rectangle sample block that middle part is fixed with leather sample;
(6) solidification rectangle sample block step (5) obtained puts into 60 DEG C of thermostatic drying chamber Post RDBMS 2-3 hour;
(7) each face of rectangle sample block good for Post RDBMS is polished flat, and relative two sides is parallel, and the one side flint glass paper observing end with leather is polished to the leather cross section of exposing needs and observing;
(8) the sample block cross section waterproof abrasive paper exposing leather observation end in step (7) is carried out gradient polishing, and after polishing, choose from the some x of two close to leather sample, y, measure rectangle sample block x respectively with micron milscale, the thickness at y point place, to microscope, then observe the picture obtaining this leather cross section, first of i.e. this sample sequence picture, notes choosing the position having pre-buried label;
(9) the rectangle sample block cross section of having clapped picture is polished off required certain thickness with 3000 order waterproof abrasive paper vertical cross-sections, polishing, rectangle sample block x is measured respectively with micron milscale, after the thickness (guaranteeing that with x, y point in step (8) be same position) at y point place, to microscope, obtain the second pictures;
(10) repeat step (9), obtain the 3rd pictures, the 4th pictures successively ..., thus obtain sequence of pictures;
(11) spacing distance of adjacent two sections (picture) made by meter of the thickness difference of the twice x point in front and back and the thickness difference of y point being averaged, thus obtains the spacing of sequence of pictures.
The epoxy resin of described step (1) is the epoxy resin that viscosity is low, the transparency is good.
The epoxy hardener of described step (1) is the agent of amine transparent cured.
The leather sample of described step (2) is the leather bar of long 10 ~ 20mm, wide 1 ~ 2mm.
The filiform of described step (4) is tinsel or macromolecular fibre, its even thickness, and cross section is circular, and diameter is less than 30 microns, has certain intensity.
The mould of described step (5) is long 50mm, wide 40mm, high 20mm oblong barrel shape mould.
Gradient polishing in described step (8) is for use 200 orders, 320 orders, 600 orders, 1000 orders, 1500 orders, 2000 orders, 3000 order liquid honings successively.
100 ~ 300 revs/min of buffing machine polishings 1 ~ 2 minute is finished in described step (8) and (9).
Beneficial effect
Method of the present invention, fixing leather fiber is embedded owing to having used epoxy resin gradation, keep the original attitude of leather fiber in woven network completely, be that sequence of pictures registration is ready by pre-buried two rhizoid shape marks, by measuring the interlamellar spacing of the thickness determination sequence of pictures of matrix, thus for accurately reflecting in subsequent treatment that leather fiber braiding structure is laid a solid foundation.In addition, final embedded samples be rectangle can ensure better each measurement point x, y and the position be placed on microscope example bench identical, because 3000 order sand paper are very thin, the thickness polished off can be controlled more accurately, be not clamminess in selected epoxy resin cure rear surface, the transparency is good, and set time is adjustable, this all facilitates operation, improves accuracy.
Accompanying drawing explanation
Fig. 1 is the structural representation of sample described in embodiment;
Fig. 2 is the structural representation solidifying rectangle sample block described in embodiment.
Embodiment
Below in conjunction with embodiment, the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Obtain a method for leather sample cross section sequence of pictures, its step is as follows:
(1) 25 grams of 593# amine transparent epoxy hardening agent are added in 100 grams of E51 epoxy resin stir, be put into and shaking table vibrates 5 minutes discharge the air be mixed into, obtain resin adhesive liquid;
(2) in resin adhesive liquid batten immersion step (1) of dried ox-hide leather cutting growth 20mm, wide 2mm prepared, leather sample is made fully to suck resin by stirring collision, then take out sample, put into 60 DEG C of thermostatic drying chamber solidifications 2 hours, obtain solidification sample block;
(3) by smooth with sand papering for each of the sample block be cured, and entirely inwardly polished in two less for vertical range opposite A faces and B face (as shown in Figure 1), until expose the leather surface position of needs observation;
(4) the sample block that step (3) has been polished is put into two exceptionally straight filamentary silver (even thickness, cross section is circular, diameter is 25 microns) in the middle of, filiform position is regulated to make the A face of sample block and B face central authorities be close to filamentary silver, then on sample block and filamentary silver, evenly drip the resin adhesive liquid that step (1) prepares, filiform is covered by resin completely, and after waiting its spontaneous curing, filamentary silver is fixed on sample block;
(5) filamentary silver is cut off, take off the sample block in step (4), sample block two ends are smooth with 180 order sand paperings, and will end (a end) sand papering be observed to the leather position of exposing needs observation, with 502 glue, a end is fixed to long 50mm, wide 40mm, the long 50mm of oblong barrel shape of high 20mm, wide 40mm, the bottom centre position of high 20mm rectangle barreled mould, makes batten vertically stand in mould central authorities, the resin adhesive liquid that implantation step (1) is obtained in mould, solidification, the demoulding at normal temperatures, obtains the rectangle sample block that middle part is fixed with leather sample;
(6) the rectangle sample block that step (5) obtains is put into 60 DEG C of thermostatic drying chamber Post RDBMS 2.5 hours;
(7) each face of rectangle sample block good for Post RDBMS is polished flat, and relative two sides is parallel, and the one side flint glass paper observing end with leather is polished to the leather cross section of exposing needs and observing;
(8) gradient polishing is carried out with 200 orders, 320 orders, 600 orders, 1000 orders, 1500 orders, 2000 orders, 3000 order waterproof abrasive papers successively in the sample block cross section of exposing leather observation end in step (7), and polishing 1.5 minutes under the rotating speed of 200 revs/min, after polishing, choose from the some x of two close to leather sample, y, rectangle sample block x is measured respectively with micron milscale, the thickness at y point place, then to microscope, observe the picture obtaining this leather cross section, namely obtain first of this sample sequence picture, note choosing the position having pre-buried label;
(9) the rectangle sample block cross section having obtained picture is polished off 10 microns thickness with 3000 order waterproof abrasive paper vertical cross-sections, polishing, measure the thickness (guaranteeing that with x, y point in step (8) be same position) at rectangle sample block x, y point place respectively with micron milscale after, to microscope, obtain the second pictures;
(10) repeat step (9), obtain the 3rd pictures, the 4th pictures successively ..., thus obtain sequence of pictures;
(11) spacing distance of adjacent two sections (picture) made by meter of the thickness difference of the twice x point in front and back and the thickness difference of y point being averaged, thus obtains the spacing of sequence of pictures.
Above example is only for illustration of the use of the present invention, but not limitation of the present invention, person skilled in the relevant technique, without departing from the spirit and scope of the present invention, various conversion or modification can also be made, therefore all equivalent technical schemes also should belong to category of the present invention, should be limited by each claim.
Claims (7)
1. obtain a method for leather cross section sequence of pictures, its step is as follows:
(1) epoxy hardener of 20-30 mass parts is added in 100 parts of epoxy resin stir, be put into and shaking table vibrates 5 minutes discharge the air be mixed into, obtain resin adhesive liquid;
(2) leather sample is immersed in the resin adhesive liquid that step (1) prepares, making leather sample fully suck resin by stirring collision, then taking out sample, put into 60 DEG C of thermostatic drying chambers solidification 2-3 hour, obtain solidifying sample block;
(3) by smooth with sand papering for each of the sample block be cured, and entirely inwardly polished in two less for vertical range opposite A faces and B face (as shown in Figure 1), until expose the leather surface position of needs observation;
(4) the sample block that step (3) has been polished is put in the middle of two exceptionally straight filiforms, filiform position is regulated to make A and the B face central authorities of sample block be close to filiform, then on sample block and filiform, evenly drip the resin adhesive liquid that step (1) prepares, filiform is covered by resin completely, be fixed on sample block Deng filiform after its spontaneous curing, described filiform is tinsel or macromolecular fibre, its even thickness, cross section is circular, diameter is less than 30 microns, has certain intensity;
(5) filiform is cut off, take off the sample block in step (4), by smooth for the two ends sand papering of sample block, then be vertically fixed in mould, the resin adhesive liquid that implantation step (1) is obtained in mould, solidification, the demoulding at normal temperatures, obtains the solidification rectangle sample block that middle part is fixed with leather sample;
(6) solidification rectangle sample block step (5) obtained puts into 60 DEG C of thermostatic drying chamber Post RDBMS 2-3 hour;
(7) each face of rectangle sample block good for Post RDBMS is polished flat, and relative two sides is parallel, and the one side flint glass paper observing end with leather is polished to the leather cross section of exposing needs and observing;
(8) the sample block cross section waterproof abrasive paper exposing leather observation end in step (7) is carried out gradient polishing, and after polishing, choose from the some x of two close to leather sample, y, measure rectangle sample block x respectively with micron milscale, the thickness at y point place, to microscope, then observe the picture obtaining this leather cross section, first of i.e. this sample sequence picture, notes choosing the position having pre-buried label;
(9) the rectangle sample block cross section of having clapped picture is polished off required certain thickness with 3000 order waterproof abrasive paper vertical cross-sections, polishing, measure the thickness (guaranteeing that with x, y point in step (8) be same position) at rectangle sample block x, y point place respectively with micron milscale after, to microscope, obtain the second pictures;
(10) repeat step (9), obtain the 3rd pictures, the 4th pictures successively ..., thus obtain sequence of pictures;
(11) spacing distance of adjacent two sections (picture) made by meter of the thickness difference of the twice x point in front and back and the thickness difference of y point being averaged, thus obtains the spacing of sequence of pictures.
2. the method obtaining leather cross section sequence of pictures as claimed in claim 1, is characterized in that, the epoxy resin of described step (1) is the epoxy resin that viscosity is low, the transparency is good.
3. the method obtaining leather cross section sequence of pictures as claimed in claim 1, it is characterized in that, the epoxy hardener of described step (1) is the agent of amine transparent cured.
4. the method obtaining leather cross section sequence of pictures as claimed in claim 1, is characterized in that, the leather sample of described step (2) is the leather bar of long 10 ~ 20mm, wide 1 ~ 2mm.
5. the method obtaining leather cross section sequence of pictures as claimed in claim 1, it is characterized in that, the mould of described step (5) is long 50mm, wide 40mm, high 20mm oblong barrel shape mould.
6. the method obtaining leather cross section sequence of pictures as claimed in claim 1, it is characterized in that, the gradient polishing in described step (8) is for use 200 orders, 320 orders, 600 orders, 1000 orders, 1500 orders, 2000 orders, 3000 order liquid honings successively.
7. the as claimed in claim 1 method obtaining leather cross section sequence of pictures, is characterized in that, is finished to 100 ~ 300 revs/min of buffing machine polishings 1 ~ 2 minute in described step (8) and (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310670321.8A CN103674953B (en) | 2013-12-11 | 2013-12-11 | A kind of method obtaining leather sample cross section sequence of pictures |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310670321.8A CN103674953B (en) | 2013-12-11 | 2013-12-11 | A kind of method obtaining leather sample cross section sequence of pictures |
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| CN103674953A CN103674953A (en) | 2014-03-26 |
| CN103674953B true CN103674953B (en) | 2016-03-16 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102305736A (en) * | 2011-06-29 | 2012-01-04 | 山东轻工业学院 | Method for preparing leather sample observed by metallurgical microscope |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102305736A (en) * | 2011-06-29 | 2012-01-04 | 山东轻工业学院 | Method for preparing leather sample observed by metallurgical microscope |
Non-Patent Citations (3)
| Title |
|---|
| 层切图像法反求工程包埋材料的研制;王江良等;《新技术新工艺 材料与表面处理》;20040531(第5期);全文 * |
| 树脂包埋技术在纤维截面切片中的应用;宗亚宁等;《纺织学报》;20070228;第28卷(第2期);第9页右栏第1段-第3段 * |
| 环氧树脂包埋法和图像分析技术在纸页Z向结构研究中的应用;洪义梅等;《天津造纸》;20111231(第4期);全文 * |
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