CN103665125A - HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof - Google Patents
HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof Download PDFInfo
- Publication number
- CN103665125A CN103665125A CN201310657131.2A CN201310657131A CN103665125A CN 103665125 A CN103665125 A CN 103665125A CN 201310657131 A CN201310657131 A CN 201310657131A CN 103665125 A CN103665125 A CN 103665125A
- Authority
- CN
- China
- Prior art keywords
- sequence
- protein
- dna
- microorganism
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof. The provided protein is the following (a) or (b) or (c): (a) protein comprising the amino acid sequence shown in the sequence 1 of a sequence table; (b) protein derived from the protein of the sequence 1 by substituting and/or deleting and/or adding one or multiple amino acid residues related to the anti-oxidation capability of the plate from the amino acid sequence of the sequence 1; and (c) protein derived from the protein of the sequence 1 by substituting and/or deleting and/or adding one or multiple amino acid residues related to the anti-oxidation capability of microbes from the amino acid sequence of the sequence 1. The provided protein participates in the oxidative stress resistant response of plants or microbes. The provided protein and the coding gene thereof play an important role for improving the oxidative stress resistant capability of plants or microbes and improving hevea brasiliensis yield or reducing hevea brasiliensis dead bark.
Description
Technical field
The invention belongs to biological technical field, be specifically related to HbsHsp1 albumen and encoding gene and the application in a kind of rubber tree source.
Background technology
The same iron and steel of natural rubber, oil, coal are also called four large industrial raw material, are basic industry and the grand strategy goods and materials that involve the interests of the state and the people.Rubber yielding plant has kind more than 2000 in the world, Para rubber tree (Hevea brasiliensis Mull.Arg.) has that gum yield is high, quality better, economical life are long, adopt the features such as the convenient and production cost of glue is low, thereby become unique a kind of tame rubber yielding plant, by the natural rubber of its production, account for the more than 90% of Natural Rubber in The World ultimate production.
Para rubber tree originates in South America Amazon River basin, belongs to the high megaphanerophyte in the typical torrid zone.China belongs to the non-traditional glue region of planting, and rubber tree is often subject to the abiotic stress condition impacts such as rubber tapping, low temperature, arid, windburn, causes rubber tree yield reducation.China plants that glue region is limited and per unit area yield is relatively low, but growing to the demand of natural rubber, this causes China's natural rubber imbalance between supply and demand to become increasingly conspicuous.Since 2009, China's natural rubber degree of self-sufficiency is less than 20%.With regard to the current caoutchouc industry present situation of China, improving per unit area yield is the only way that ensures natural rubber supply capacity.
In affecting the factor of natural rubber per unit area yield, dead skin is major limitation sex factor.At present, China's dead skin accumulative total incidence is between 20%-40%, and serious area is more than 40%.Along with the popularization of high yield clone and ethrel tapping with stimulus technology, dead skin incidence and severity are ascendant trend year by year.Compare with healthy rubber tree, on dead skin tree corpora flava film, nadh oxidase, peroxidase activity strengthen, and SOD and CAT reduced activity, the oxidation inhibitor concentration such as xitix and reduced form mercaptan reduce greatly, active oxygen and quinones substance accumulate in a large number, cause corpora flava to break and discharge coagulation factors, finally causing latex coagulation, stopping up latex dust and cause dead skin.In dead skin generating process, active oxygen produces and removes the unbalance accumulated active oxygen causing is dead skin generation prerequisite and basis.In view of above result of study, improve the genetic expression of rubber tree anti-oxidant activity, remove in time the too much active oxygen of accumulation, keeping reactive oxygen species to produce and remove balance may be to avoid the effective way of rubber tree generation dead skin.In addition, effective removing of rubber tree ROS is also a major reason of rubber tree high yield.
Summary of the invention
The object of this invention is to provide HbsHsp1 albumen and encoding gene and the application in a kind of rubber tree source.
Protein provided by the invention, available from rubber tree (Hevea brasiliensis), called after HbsHsp1 albumen is following (a) or (b) or (c):
(a) protein that the aminoacid sequence shown in sequence 1 forms in sequence table;
(b) replacement and/or disappearance and/or interpolation and the protein that by sequence 1 derived relevant to plant anti-oxidation ability through one or several amino-acid residue by the aminoacid sequence of sequence 1;
(c) replacement and/or disappearance and/or interpolation and the protein that by sequence 1 derived relevant to microorganism resistance of oxidation through one or several amino-acid residue by the aminoacid sequence of sequence 1.
In order to make the protein in (a) be convenient to purifying, the N-terminal of the protein that can form at the aminoacid sequence shown in sequence in sequence table 1 or C-terminal connect label as shown in table 1.
The sequence of table 1 label
| Label | Residue | Sequence |
| Poly-Arg | 5-6(is generally 5) | RRRRR |
| Poly-His | 2-10(is generally 6) | HHHHHH |
| FLAG | 8 | DYKDDDDK |
| Strep-tag?II | 8 | WSHPQFEK |
| c-myc | 10 | EQKLISEEDL |
Above-mentioned (b) or (c) in protein can synthetic, also can first synthesize its encoding gene, then carry out biological expression and obtain.Above-mentioned (b) or (c) in the encoding gene of protein can be by lacking the codon of one or several amino-acid residue in the DNA sequence dna shown in sequence in sequence table 2, and/or carry out the missense mutation of one or several base pair, and/or the encoding sequence that connects the label shown in table 1 at its 5 ' end and/or 3 ' end obtains.
The gene of code for said proteins (HbsHsp1 gene) also belongs to protection scope of the present invention.
Described gene specifically can be the DNA molecular of (1) or (2) or (3) or (4) or (5) or (6) as follows:
(1) coding region is if the sequence 2 of sequence table is from the DNA molecular as shown in 5 ' end 28-522 position Nucleotide;
(2) DNA molecular shown in the sequence 2 of sequence table;
(3) the DNA sequence dna hybridization limiting with (1) or (2) under stringent condition and the DNA molecular of the anti-oxidant associated protein of coded plant;
(4) the DNA sequence dna hybridization limiting with (1) or (2) under stringent condition and the DNA molecular of the anti-oxidant associated protein of coding microorganism;
(5) DNA sequence dna limiting with (1) or (2) at least has 70%, at least have 75%, at least have 80%, at least have 85%, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least have a DNA molecular of 99% homology and the anti-oxidant associated protein of coded plant;
(6) DNA sequence dna limiting with (1) or (2) at least has 70%, at least have 75%, at least have 80%, at least have 85%, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least have a DNA molecular of 99% homology and the anti-oxidant associated protein of coding microorganism.
Above-mentioned stringent condition can be at 6 * SSC, in the solution of 0.5%SDS, under 65oC, hybridizes, and then uses 2 * SSC, 0.1%SDS and 1 * SSC, 0.1%SDS respectively to wash film once.
The expression cassette that contains described HbsHsp1 gene, recombinant vectors, transgenic cell line or recombinant bacterium all belong to protection scope of the present invention.
The recombinant expression vector that available existing expression vector establishment contains described gene.Described expression vector also can comprise 3 ' end untranslated region of foreign gene, comprises the DNA fragmentation of polyadenylic acid signal and any other participation mRNA processing or genetic expression.The bootable polyadenylic acid of described polyadenylic acid signal joins 3 ' end of mRNA precursor.While using described gene constructed recombinant expression vector, before its transcription initiation Nucleotide, can add any enhancement type promotor or constitutive promoter, they can be used alone or are combined with other promotor; In addition, while using gene constructed recombinant expression vector of the present invention, also enhanser be can use, translational enhancer or transcriptional enhancer comprised, but must be identical with the reading frame of encoding sequence, to guarantee the correct translation of whole sequence.The source of described translation control signal and initiator codon is widely, can be natural, also can synthesize.Translation initiation region can be from transcription initiation region or structure gene.For the ease of identifying and screening, can process described recombinant expression vector, as add coding can produce the enzyme of colour-change or the gene of luminophor, there is the antibiotic marker thing of resistance or anti-chemical reagent marker gene etc.Described recombinant vectors specifically can be the recombinant plasmid that the multiple clone site of described HbsHsp1 gene insertion pET28a (+) carrier is obtained.
Described recombinant bacterium specifically can be described recombinant plasmid is imported to the recombinant bacterium that intestinal bacteria obtain.Described intestinal bacteria specifically can be intestinal bacteria Transetta (DE3).
The present invention also protects a kind of method of cultivating recombinant microorganism, is described HbsHsp1 gene is imported in object microorganism, obtains Oxidative Stress ability higher than the recombinant microorganism of described object microorganism.Described object microorganism can be intestinal bacteria, specifically can be intestinal bacteria Transetta (DE3).Described oxidative stress specifically can be Hydrogen Peroxide Stress.
The primer pair of described HbsHsp1 full length gene or its any fragment of increasing also belongs to protection scope of the present invention.
The present invention also protects the application of described HbsHsp1 albumen, is following (I) or (II): (I) involved in plant or microorganism are replied oxidative stress; (II) improves plant or the resistance of microorganism to oxidative stress.Described microorganism can be intestinal bacteria, specifically can be intestinal bacteria Transetta (DE3).Described oxidative stress specifically can be Hydrogen Peroxide Stress.
The present inventor is based on to rubber tree Active oxygen generation and clearing and the result of study to output and dead skin thereof, found HbsHsp1 albumen, through experimental identification, this albumen can improve the resistance of intestinal bacteria to oxidative stress, strengthen its surviving rate under hydrogen peroxide treatment condition, thereby determine that this albumen has anti-oxidant activity, can remove rubber tree activity in vivo oxygen.Protein provided by the invention and encoding gene thereof will play a significant role in improving the ability of plant or microorganism Oxidative Stress, improve rubber tree output or reducing rubber tree dead skin.
Accompanying drawing explanation
Fig. 1 is the expression pattern analysis of HbsHsp1 gene in different tissues.
Fig. 2 is the expression pattern analysis of HbsHsp1 gene under hydrogen peroxide treatment condition.
Fig. 3 is SDS-PAGE collection of illustrative plates.
Fig. 4 is the anti-oxidant activity qualification result of recombinant bacterium and contrast bacterium.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, and result is expressed as mean+SD.
Para rubber tree heat is ground 7-33-97: plant in Chinese Academy of Tropical Agricultural Sciences testing ground and national rubber tree Germplasm Resources.PET28a (+) carrier: NOVAGEN company, production number is 69864-3.Intestinal bacteria Transetta (DE3), weighs up again and sends out bacterium: NOVAGEN company, production number is 69450-3.
The acquisition of embodiment 1, HbsHsp1 albumen and encoding gene thereof
Set up rubber tree bark and transcribe group degree of depth sequencing data storehouse, from Para rubber tree heat, grind and 7-33-97, find one section of 632bp EST fragment, this EST fragment, as shown in the sequence 2 of sequence table, comprises complete open reading frame, the protein shown in the sequence 1 of code sequence list.
By the protein called after HbsHsp1 albumen shown in the sequence of sequence table 1.By the unnamed gene of coding HbsHsp1 albumen, be HbsHsp1 gene, its open reading frame is if the sequence 2 of sequence table is from as shown in 5 ' end 28-522 position Nucleotide.
Get respectively bark, latex, blade, male flower, female flower and flower pesticide that Para rubber tree heat is ground 7-33-97, extracting total RNA reverse transcription is cDNA, adopts the relative expression quantity of the primer pair detection HbsHsp1 gene of F1 and R1 composition.The results are shown in Figure 1.Result shows, HbsHsp1 genetic expression inorganization specificity is expressed the highlyest in blade, expresses minimum in flower pesticide.
Select Para rubber tree heat to grind 7-33-97 and do not open that to cut treelet be experiment material, carry out H2O2 processing (reference literature " Zhu JH; Zhang QQ; Wu R; et al.HbMT2, an ethephon-induced metallothionein gene from Hevea brasiliensis responds to H2O2stress.Plant Physiol Biochem, 2010; 48 (8): 1-6. " carries out), respectively at 0h, 6h, 24h and 48h after processing, get latex.Extracting total RNA reverse transcription is cDNA, adopts the relative expression quantity of the primer pair detection HbsHsp1 gene of F1 and R1 composition.The results are shown in Figure 2.Result shows, under H2O2 processes, HbsHsp1 genetic expression slightly raises at 6h, and 24h is down to minimum, after 48h, raises again.
F1：5’-catcacagccacacaatcac-3’；
R1：5’-ggaatccgtcaaatgggtcc-3’。
The functional verification of embodiment 2, HbsHsp1 albumen
One, the structure of recombinant plasmid
1, extracting Para rubber tree heat grinds total RNA the reverse transcription of 7-33-97 bark and obtains cDNA.
2, take the cDNA that step 1 obtains is template, with the primer pair that F2 and R2 form, carries out pcr amplification, obtains pcr amplification product.
F2：5’-gc
ggatcc?atgtcgatcaatcccttcttc-3’；
R2：5’-gc
gaattc?tcaaccagaaatcttaatagac-3’。
3, the pcr amplification product obtaining by restriction enzyme BamH I and EcoR I double digestion step 2, reclaims enzyme and cuts product.
4,, with restriction enzyme BamH I and EcoR I double digestion pET28a (+) carrier, reclaim the carrier framework of about 5400bp.
5, enzyme step 3 being obtained is cut the carrier framework that product obtains with step 4 and is connected, and obtains recombinant plasmid pET28a-HbsHsp1.According to sequencing result, recombinant plasmid pET28a-HbsHsp1 is carried out to structrual description as follows: the sequence 2 of having inserted sequence table between the BamH I of pET28a (+) carrier and EcoR I restriction enzyme site is from the double chain DNA molecule shown in 5 ' end 28-522 position Nucleotide.
Two, the structure of recombinant bacterium
Recombinant plasmid pET28a-HbsHsp1 is imported to intestinal bacteria Transetta (DE3), obtain recombinant bacterium.
PET28a (+) carrier is imported to intestinal bacteria Transetta (DE3), obtain contrasting bacterium.
Recombinant bacterium and contrast bacterium are tested respectively as follows: (1) gets single bacterium colony, be seeded to 3mL containing the LB liquid nutrient medium of 100mg/L kantlex, shaking culture is spent the night; (2) get the bacterium liquid that step (1) obtains, by the volume ratio of 1:100, be forwarded to the liquid LB substratum containing 100mg/L kantlex, 37 ℃, 200rpm/min shaking culture are to OD
600nm=0.4 left and right; (3), after completing steps (2), adding IPTG is 1mmol/L to concentration, 37 ℃, 200rpm/min shaking culture (induction) certain hour; (4) after completing steps (3), centrifugal collection thalline, adds isopyknic 2 * sample-loading buffer, and after ultrasonication, 95 ℃ are boiled 5min and make protein denaturation, and then the centrifugal 1min of 12000rpm/min, collects supernatant liquor; (5) supernatant liquor step (4) being obtained carries out SDS-PAGE.
Fig. 3 is shown in by SDS-PAGE collection of illustrative plates, swimming lane 1 is for contrasting the supernatant liquor before bacterium induction, swimming lane 2 is the supernatant liquor of contrast bacterium induction after 6 hours, swimming lane 3 is the supernatant liquor of recombinant bacterium induction after 6 hours, swimming lane 4 is the supernatant liquor of recombinant bacterium induction after 4 hours, swimming lane 5 is the supernatant liquor of recombinant bacterium induction after 2 hours, swimming lane 6 is the supernatant liquor before recombinant bacterium induction, and swimming lane M is that the molecular weight of molecular weight of albumen Marker(under upper is followed successively by 170kDa, 130kDa, 100kDa, 70kDa, 55kDa, 40kDa, 35kDa, 25kDa and 15kDa).Result shows: after IPTG induction, recombinant bacterium is expressed the albumen of 20kD left and right, basically identical with the expection molecular weight of HbsHsp1 albumen; Before IPTG induction and after IPTG induction, contrast bacterium is not all expressed the albumen of corresponding molecular weight.
Three, the anti-oxidant activity of recombinant bacterium and contrast bacterium is identified
By recombinant bacterium, contrast bacterium and the bacterium that sets out is tested respectively as follows: (1) gets single bacterium colony, is seeded to LB liquid nutrient medium, 37 ℃ of shaking culture overnight incubation; (2) get the bacterium liquid that 0.1mL step (1) obtains, be seeded to 100mL containing in the liquid LB substratum of 100 μ g/mL kantlex, 37 ℃, 200rpm vibrate to OD
600nm=0.3; (3) get the bacterium liquid that step (2) obtains, be divided into two groups, first group adds hydrogen peroxide and make its concentration is 0.2mM, second group adds hydrogen peroxide and IPTG, makes the concentration of hydrogen peroxide is that the concentration of 0.2mM, IPTG is 1mmol/L, 37 ℃, 200rpm shaking culture, respectively at shaking culture 0 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours and 8 hours, sampling detected the OD of bacterium liquid
600nmvalue.
First group the results are shown in Figure 4A.Under the condition without IPTG induction, recombinant bacterium, contrast bacterium and set out the OD of bacterium at each time point
600nmbe worth basic identically, show that both somatic cells density is basically identical.
Second group the results are shown in Figure 4B.Under the condition of IPTG induction, at each time point, the OD of recombinant bacterium
600nmvalue is all significantly higher than contrast bacterium, the OD of contrast bacterium and the bacterium that sets out
600nmbe worth basic identical.Result shows, under the condition of IPTG induction, recombinant bacterium to the tolerance of Hydrogen Peroxide Stress higher than contrast bacterium and the bacterium that sets out.Can draw the following conclusions: HbsHsp1 albumen has the effect of Oxidative Stress.
Claims (10)
1. a protein is following (a) or (b) or (c):
(a) protein that the aminoacid sequence shown in sequence 1 forms in sequence table;
(b) replacement and/or disappearance and/or interpolation and the protein that by sequence 1 derived relevant to plant anti-oxidation ability through one or several amino-acid residue by the aminoacid sequence of sequence 1;
(c) replacement and/or disappearance and/or interpolation and the protein that by sequence 1 derived relevant to microorganism resistance of oxidation through one or several amino-acid residue by the aminoacid sequence of sequence 1.
2. the gene of protein described in the claim 1 of encoding.
3. gene as claimed in claim 2, is characterized in that: described gene is the DNA molecular of following (1) or (2) or (3) or (4) or (5) or (6):
(1) coding region is if the sequence 2 of sequence table is from the DNA molecular as shown in 5 ' end 28-522 position Nucleotide;
(2) DNA molecular shown in the sequence 2 of sequence table;
(3) the DNA sequence dna hybridization limiting with (1) or (2) under stringent condition and the DNA molecular of the anti-oxidant associated protein of coded plant;
(4) the DNA sequence dna hybridization limiting with (1) or (2) under stringent condition and the DNA molecular of the anti-oxidant associated protein of coding microorganism;
(5) DNA sequence dna limiting with (1) or (2) at least has 70%, at least have 75%, at least have 80%, at least have 85%, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least have a DNA molecular of 99% homology and the anti-oxidant associated protein of coded plant;
(6) DNA sequence dna limiting with (1) or (2) at least has 70%, at least have 75%, at least have 80%, at least have 85%, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least have a DNA molecular of 99% homology and the anti-oxidant associated protein of coding microorganism.
4. the expression cassette, recombinant vectors, transgenic cell line or the recombinant bacterium that contain gene described in claim 2 or 3.
5. recombinant vectors as claimed in claim 4, is characterized in that: the recombinant plasmid that described recombinant vectors obtains for gene described in claim 2 or 3 being inserted to the multiple clone site of pET28a (+) carrier.
6. recombinant bacterium as claimed in claim 4, is characterized in that: described recombinant bacterium is for importing by recombinant plasmid described in claim 5 recombinant bacterium that intestinal bacteria obtain.
7. cultivating a method for recombinant microorganism, is that gene described in claim 2 or 3 is imported in object microorganism, obtains Oxidative Stress ability higher than the recombinant microorganism of described object microorganism.
8. method as claimed in claim 7, is characterized in that: described object microorganism is intestinal bacteria.
9. the primer pair of full length gene or its any fragment described in the claim 2 or 3 that increases.
10. the application of albumen described in claim 1, is following (I) or (II): (I) involved in plant or microorganism are replied oxidative stress; (II) improves plant or the resistance of microorganism to oxidative stress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310657131.2A CN103665125B (en) | 2013-12-06 | 2013-12-06 | HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310657131.2A CN103665125B (en) | 2013-12-06 | 2013-12-06 | HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103665125A true CN103665125A (en) | 2014-03-26 |
| CN103665125B CN103665125B (en) | 2015-05-06 |
Family
ID=50303985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310657131.2A Expired - Fee Related CN103665125B (en) | 2013-12-06 | 2013-12-06 | HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103665125B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105254729A (en) * | 2015-11-12 | 2016-01-20 | 中国热带农业科学院橡胶研究所 | Rubber tree tapping panel dryness associated protein HbMC1 and encoding gene and application thereof |
| CN105273070A (en) * | 2015-11-12 | 2016-01-27 | 中国热带农业科学院橡胶研究所 | Tapping panel dryness associated protein HbMC2 and encoding gene and application thereof |
| CN110117604A (en) * | 2019-04-29 | 2019-08-13 | 贵州大学 | A kind of recombinant vector and expression of tea tree heat shock protein CssHSP-6 gene |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102786588A (en) * | 2012-08-21 | 2012-11-21 | 中国热带农业科学院橡胶研究所 | Rubber plant translationally controlled tumor protein, encoding gene thereof and application of rubber plant translationally controlled tumor protein |
-
2013
- 2013-12-06 CN CN201310657131.2A patent/CN103665125B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102786588A (en) * | 2012-08-21 | 2012-11-21 | 中国热带农业科学院橡胶研究所 | Rubber plant translationally controlled tumor protein, encoding gene thereof and application of rubber plant translationally controlled tumor protein |
Non-Patent Citations (1)
| Title |
|---|
| ZHU J ET AL.: "HbMT2, an ethephon-induced metallothionein gene from Hevea brasiliensis responds to H2O2 stress", 《PLANT PHYSIOL BIOCHEM》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105254729A (en) * | 2015-11-12 | 2016-01-20 | 中国热带农业科学院橡胶研究所 | Rubber tree tapping panel dryness associated protein HbMC1 and encoding gene and application thereof |
| CN105273070A (en) * | 2015-11-12 | 2016-01-27 | 中国热带农业科学院橡胶研究所 | Tapping panel dryness associated protein HbMC2 and encoding gene and application thereof |
| CN105273070B (en) * | 2015-11-12 | 2018-05-15 | 中国热带农业科学院橡胶研究所 | Rubber tree dead skin GAP-associated protein GAP HbMC2 and its encoding gene and application |
| CN105254729B (en) * | 2015-11-12 | 2018-05-18 | 中国热带农业科学院橡胶研究所 | Rubber tree dead skin GAP-associated protein GAP HbMC1 and its encoding gene and application |
| CN110117604A (en) * | 2019-04-29 | 2019-08-13 | 贵州大学 | A kind of recombinant vector and expression of tea tree heat shock protein CssHSP-6 gene |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103665125B (en) | 2015-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101173002B (en) | Plants stress tolerance correlation transcription factor GmWRKY54, encoding gene and application thereof | |
| CN101914147B (en) | Plant disease resistance-related protein and coding gene and application thereof | |
| CN101906155A (en) | Protein ErNAC7 related to drought and salt resistance of plants and coding gene and application thereof | |
| CN102766610B (en) | Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof | |
| CN103665125B (en) | HbsHsp1 protein coming from hevea brasiliensis, and coding gene and application thereof | |
| CN105111295B (en) | The breeding method and its relevant biological material for turning WMYB-R DNA triticums of anti-root rot and banded sclerotial blight | |
| CN104046636A (en) | Codon vegetalization-transformed PMI gene and applications thereof | |
| CN102277342A (en) | Mannose and mutants thereof | |
| CN103665126B (en) | The HbsHsp2 albumen in rubber tree source and encoding gene and application | |
| CN103184206A (en) | Mannase and mutants thereof | |
| CN104480120A (en) | Plant salt-tolerant related gene PpSIG2 as well as encoding protein and application thereof | |
| CN101412990B (en) | Leymus chinensis fructan hydrolases, and encoding genes and use thereof | |
| WO2008153272A1 (en) | Protease having algicidal activity, gene encoding the same and algicidal formulation comprising the same | |
| CN101712718B (en) | Protein relevant to plant drought resistance, coding gene and application thereof | |
| CN102796186A (en) | Protein associated with DNA damage repair and coding gene and application thereof | |
| CN102533683B (en) | Phyllostachys edulis violaxanthin deepoxidase (PeVDE) protein, coding gene thereof and applications thereof | |
| CN102245763A (en) | Acidothermus celluloyticus xylanase | |
| CN103484487B (en) | A kind of small cabbage moth N,O-Diacetylmuramidase II and preparation method thereof and application | |
| CN103849605A (en) | Method for cultivating resistant plant, and special protein and gene thereof | |
| CN105505895A (en) | Anti-glyphosate associated protein derived from candida palmioleophila as well as encoding gene and application thereof | |
| CN102295689B (en) | Plant drought tolerance related protein AtDi19, and coding gene and application thereof | |
| CN104928273A (en) | Garlic allinase, encoding gene thereof and application of garlic allinase | |
| CN103739687B (en) | Antioxidative protein from rubber tree as well as encoding gene and application of antioxidative protein | |
| CN102604906A (en) | Bombyx mori glutathione-S-transferase BmGSTD4 and genes thereof | |
| CN102618521A (en) | ATP(Adenosine Triphosphate) -dependent metalloproteinase FtsH (Filamentation temperature-sensitive H |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150506 Termination date: 20211206 |