CN103654996A - Special method and device for rat intraperitoneal injection - Google Patents
Special method and device for rat intraperitoneal injection Download PDFInfo
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- CN103654996A CN103654996A CN201310598852.0A CN201310598852A CN103654996A CN 103654996 A CN103654996 A CN 103654996A CN 201310598852 A CN201310598852 A CN 201310598852A CN 103654996 A CN103654996 A CN 103654996A
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- rat
- top cover
- equipment
- riser
- intraperitoneal injection
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000007928 intraperitoneal injection Substances 0.000 title claims abstract description 17
- 239000003814 drug Substances 0.000 claims abstract description 18
- 210000000683 abdominal cavity Anatomy 0.000 claims abstract description 12
- 210000001217 buttock Anatomy 0.000 claims abstract description 5
- 210000001015 abdomen Anatomy 0.000 claims abstract description 4
- 241000700159 Rattus Species 0.000 claims description 89
- 239000007924 injection Substances 0.000 claims description 15
- 238000002347 injection Methods 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000004044 response Effects 0.000 abstract description 3
- 238000005086 pumping Methods 0.000 abstract 1
- 230000003938 response to stress Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 230000035882 stress Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 210000004247 hand Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229940035674 anesthetics Drugs 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 239000003193 general anesthetic agent Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000004932 little finger Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 208000030212 nutrition disease Diseases 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
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Abstract
The invention provides a special method and device for rat intraperitoneal injection, wherein the method and equipment are low in rat stress and emergency response, safe, reliable and convenient to use. The method comprises the following steps that (1) according to the actual weight of a rat, a top cover in the corresponding size is selected; (2) an injector is used for pumping medicine inside, the rat is placed at a tunnel channel portal of the equipment and the rat can walk into the tunnel channel automatically; (3) after the rat enters the tunnel channel, the rat can walk or run forwards on a conveying mechanism by instinct, the tail of the rat is dragged and lifted up to prevent the rat from escaping forward, and the buttocks of the rat are made to turn upwards by about 15 degrees to 30 degrees; (4) the injector is used, a syringe needle is made to tilt upwards slightly and pierce into the position which is 1cm away from the right side of the lower middle abdomen, when an operator feels that the syringe needle drops obviously, the medicine is injected into the abdominal cavity quickly, and after the medicine is completely injected, the syringe needle is withdrawn quickly. The equipment is a channel so that the rat can move inside to a certain extent, therefore, struggling of the rat is avoided, the stress response is reduced and influences to the experiment result are reduced to the minimum.
Description
Technical field
The present invention relates to a kind of method and equipment thereof that is exclusively used in rats by intraperitoneal injection.
Background technology
Rat is one of animal of the most often using in zoopery, be usually used in the research in the fields such as nerve-endocrine, behavioristics, nutrition and metabolic disease, surgery, infectious disease, materia medica, hereditism, oncology, medical research has at present be unable to do without rat, and along with global aging is more and more serious, the research of carrying out on senile rat is also more and more.In carrying out the experiment of rat, often need to carry out the injection of abdominal cavity medicine to rat, as the injection of anesthetics, conventional study medicine.Yet at present abdominal cavity drug injection is the carrying out of with hands, rat being held or using the movable situation of the powerful restriction of rat fixing device rat mostly.The abdominal cavity drug injection carrying out under the state of these powerful restriction activities in rats, can force rat acutely to struggle, rat is caused to strong stress, the final experimental result (particularly nerve-endocrine and ethological experiment) that affects, and hands is grabbed rat and is often grabbed not firm (particularly senile rat), easily infusion of medicine abdominal cavity important blood vessels or internal organs are caused to rats death, research worker is easily bitten by rat.
Summary of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of to stress in rats the emergency reaction method that is exclusively used in rats by intraperitoneal injection and equipment thereof low, safe and reliable and easy to use.
In order to address the above problem, the present invention by the following technical solutions: a kind of method that is exclusively used in rats by intraperitoneal injection, comprises the following steps:
1. according to the ABW of rat, tune the top cover by corresponding size;
2. in syringe is pumped into medicine, rat is placed on to the passway, tunnel of this equipment, rat can be advanced into this tunnel passage certainly;
3. when rat enters after tunnel passage, rat is understood going ahead or running of instinct on connecting gear, and tractive is lived and mentioned rat tail, avoids rat to escape forward, and makes rat buttocks be upturned approximately 15 °-30 °;
4. use syringe, syringe needle is slightly inclined upwardly, and in selection, lower abdomen is other to the right opens 1cm position and thrust, when having syringe needle to have obviously to fall through sense, by medicine fast injection to abdominal cavity, the quick withdraw of the needle after completing.
Implement an equipment for said method, comprise left and right riser, be arranged on the top cover on the riser of left and right and be arranged on the connecting gear between the riser of left and right, described left and right riser, top cover and connecting gear form the tunnel passage that is applicable to holding rat.
A kind of method of rats by intraperitoneal injection and connecting gear of equipment thereof of being exclusively used in of the present invention can allow rat have movable forward ability, now rat initiatively goes ahead or runs on connecting gear, but the transmission due to connecting gear, make rat walk about or run in original place, be unlikely to is that it really advances, it is the function that connecting gear is similar to treadmill, left and right riser and top cover can prevent the rat operator that later bites, can not cause extruding to rat simultaneously yet, rat is struggled strongly, produce radical response, thereby the testing result of impact experiment, even injection site is not right, cause rats death, the activity forward of rat, make to spread its attention carrying out the abdominal cavity drug injection time-division, be convenient to complete smoothly the injection of abdominal cavity medicine, can damage research worker and rat hardly.The using method of this equipment is easy and simple to handle, and experimenter grabs rat without training exercise, can complete fast the injection of abdominal cavity medicine, shortens experimental period, and the method is applied widely, and the rat of different weight all can be used the method to carry out lumbar injection.
This equipment is a passage, and rat can carry out activity to a certain degree inside, does not limit its activity completely, avoids it to struggle, and reduces its stress, and the impact of experimental result is reduced to minimum.
Below in conjunction with the drawings and specific embodiments, a kind of method and equipment thereof that is exclusively used in rats by intraperitoneal injection of the present invention is described further.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation that is exclusively used in the equipment of rats by intraperitoneal injection provided by the invention.
Fig. 2 is the left view of Fig. 1.
Fig. 3 is the structural representation of the outer top cover in Fig. 1.
Fig. 4 is the structural representation of the socket internal head cover in Fig. 1.
The specific embodiment
As shown in Figure 1, Figure 2, Figure 3, Figure 4, a kind of a kind of specific embodiment that is exclusively used in the method for rats by intraperitoneal injection of the present invention: according to the ABW of rat, tune the top cover 2 by corresponding size, the method is applied widely, and the rat of different weight all can be used the method to carry out lumbar injection; Hands is worn glove, in 5ml syringe, medicine being pumped into, mentions rat tail, rat is placed on to 4 mouthfuls, the tunnel passage of this equipment, and rat can be advanced into this tunnel passage 4 certainly, also can push away gently its hip, makes rat enter tunnel passage 4; When rat enters after tunnel passage 4, rat is understood going ahead or running of instinct on connecting gear 3, the left hand palm and four little fingers of toe are placed in rat back near buttocks top, thumb tractive is lived and is mentioned rat tail, avoid rat to escape forward, and make rat buttocks be upturned approximately 15 °-30 °, now rat is in the Trendelenburg position, abdominal visceral browser is turned forward, damage while avoiding injecting; Right hand syringes, syringe needle is slightly inclined upwardly, in selection, lower abdomen is other to the right opens 1cm position and thrusts, when having syringe needle to have obviously to fall through sense, by medicine fast injection to abdominal cavity, the quick withdraw of the needle after completing, easy and simple to handle, experimenter grabs rat without training exercise, can complete fast the injection of abdominal cavity medicine, shortens experimental period.Said method can damage the pressure of having avoided rat to research worker and rat hardly and fix, and reduces its struggle and stress, little on experimental result impact.
As Fig. 1, Fig. 2, Fig. 3, shown in Fig. 4, a kind of a kind of specific embodiment that is exclusively used in the equipment of rats by intraperitoneal injection of the present invention: comprise left and right riser 1, be arranged on the top cover 2 on left and right plastics riser 1 and be arranged on the connecting gear 3 between left and right riser 1, left and right riser 1, top cover 2 and connecting gear 3 form the tunnel passage 4 that is applicable to holding rat, rat can walk inside, but to a certain degree limit its significantly left and right and up and down, prevent the rat operator that later bites, can not cause extruding to rat simultaneously yet, rat is struggled strongly, produce radical response, thereby the testing result of impact experiment, even injection site is not right, cause rats death.
In order to facilitate experiment to adopt, manually provide transmission motive force substitute motor, connecting gear 3 is by conveyer belt 31 and drive the roller 32 of conveyer belt 31 transmission to form.
In order to be applicable to the rat studies of different sizes, use, the large I of this tunnel passage 4 regulates by the replacement of top cover 2 large ting models, top cover 2 comprises outer top cover 2-1 and at least one socket internal head cover 2-2, the medial surface of described outer top cover 2-1 is provided with gathering sill 21, described socket internal head cover 2-2 outside is provided with gib block 22, gathering sill 21 and gib block 22 are structure wide at the top and narrow at the bottom, described gathering sill 21 matches with corresponding gib block 22, such structure is more firm when top cover 2-1 matches with socket top cover 2-2 outside, left and right riser 1 comprises riser 1-2 in outer riser 1-1 and at least one socket, outer riser 1-1 is connected with outer top cover 2-1, in socket, riser 1-2 is connected with corresponding socket internal head cover 2-2, outer top cover 2-1 and outer riser 1-1 consist of the tunnel passage 4 of maximum model, when rat build is less than tunnel passage 4 size of largest number, in top cover 2-1, in suitable socket internal head cover 2-2 of socket and suitable socket of riser 1-1 socket outside, thereby riser 1-2 forms a new tunnel passage 4 outside, these tunnel passage 4 sizes are just applicable to the corresponding rat of build, thereby reach the effect that just covers rat.
The present embodiment top cover 2 is adopted as transparent arc or semicircle, the top cover 2 transparent experimenters' of being of value to the activity phenomenon of observing rat is dealt with in time, the present embodiment top cover 2 adopts arc, certainly can also use the top cover 2 of other shape, as semicircle etc., top cover 2 employing arcs are conducive to top cover 2 and more press close to rat back, avoid it later, improve conventional efficient.
Claims (6)
1. an equipment that is exclusively used in rats by intraperitoneal injection, it is characterized in that: comprise left and right riser (1), be arranged on the top cover (2) on left and right riser (1) and be arranged on the connecting gear (3) between left and right riser (1), described left and right riser (1), top cover (2) and connecting gear (3) form the tunnel passage (4) that is applicable to holding rat.
2. a kind of equipment that is exclusively used in rats by intraperitoneal injection according to claim 1, is characterized in that: described connecting gear (3) is by conveyer belt (31) and drive the roller (32) of conveyer belt (31) transmission to form.
3. a kind of equipment that is exclusively used in rats by intraperitoneal injection according to claim 1 and 2, it is characterized in that: described top cover (2) comprises outer top cover (2-1) and at least one socket internal head cover (2-2), the medial surface of described outer top cover (2-1) is provided with gathering sill (21), described socket internal head cover (2-2) outside is provided with gib block (22), described gathering sill (21) matches with corresponding gib block (22), described left and right riser (1) comprises riser (1-2) in outer riser (1-1) and at least one socket, described outer riser (1-1) is connected with outer top cover (2-1), in described socket, riser (1-2) is connected with corresponding socket internal head cover (2-2).
4. a kind of equipment that is exclusively used in rats by intraperitoneal injection according to claim 1 and 2, is characterized in that: described top cover (2) is transparent arc or semicircle.
5. a kind of equipment that is exclusively used in rats by intraperitoneal injection according to claim 3, is characterized in that: described top cover (2) is transparent arc or semicircle.
6. a method that is exclusively used in rats by intraperitoneal injection, is characterized in that: comprise the following steps:
1. according to the ABW of rat, tune the top cover (2) by corresponding size;
2. in syringe is pumped into medicine, rat is placed on to the tunnel passage (4) mouthful of this equipment, rat can be advanced into this tunnel passage (4) certainly;
3. when rat enters after tunnel passage (4), rat going ahead or running the upper meeting of connecting gear (3) instinct, and tractive is lived and mentioned rat tail, avoids rat to escape forward, and makes rat buttocks be upturned approximately 15 °-30 °;
4. use syringe, syringe needle is slightly inclined upwardly, and in selection, lower abdomen is other to the right opens 1cm position and thrust, when having syringe needle to have obviously to fall through sense, by medicine fast injection to abdominal cavity, the quick withdraw of the needle after completing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310598852.0A CN103654996B (en) | 2013-11-25 | 2013-11-25 | A kind of equipment being exclusively used in rats by intraperitoneal injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310598852.0A CN103654996B (en) | 2013-11-25 | 2013-11-25 | A kind of equipment being exclusively used in rats by intraperitoneal injection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103654996A true CN103654996A (en) | 2014-03-26 |
| CN103654996B CN103654996B (en) | 2015-08-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310598852.0A Expired - Fee Related CN103654996B (en) | 2013-11-25 | 2013-11-25 | A kind of equipment being exclusively used in rats by intraperitoneal injection |
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| Country | Link |
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| CN (1) | CN103654996B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958119A (en) * | 2015-07-24 | 2015-10-07 | 温州医科大学附属第一医院 | Acupuncture treatment fixator providing convenience for fixing mouse |
| CN106725980A (en) * | 2016-12-30 | 2017-05-31 | 谷翠芝 | Rat Thoracicoabdominal puncture fixing device |
| CN109124810A (en) * | 2018-09-26 | 2019-01-04 | 华蓥市铜堡初级中学 | A kind of animal blood taking platform |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0847502A (en) * | 1994-08-09 | 1996-02-20 | Tsumura & Co | Retainer |
| CN201807057U (en) * | 2009-07-22 | 2011-04-27 | 李雁鹏 | Design and application of novel experimental equipment for sleep deprivation of rat |
| CN202010217U (en) * | 2011-03-24 | 2011-10-19 | 宋小莉 | Mouse fixing device |
| CN202051849U (en) * | 2011-05-13 | 2011-11-30 | 中国人民解放军第四军医大学 | Lab mouse tail intravenous injection fixing device |
| CN102451046A (en) * | 2010-10-25 | 2012-05-16 | 陈浩杰 | Fixing device for vena caudalis injection of mouse |
| CN202892144U (en) * | 2012-11-29 | 2013-04-24 | 中国人民解放军第三军医大学第一附属医院 | Inflatable rat fixer |
| CN203576689U (en) * | 2013-11-25 | 2014-05-07 | 温州医科大学附属第一医院 | Device special for rat intraperitoneal injection |
-
2013
- 2013-11-25 CN CN201310598852.0A patent/CN103654996B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0847502A (en) * | 1994-08-09 | 1996-02-20 | Tsumura & Co | Retainer |
| CN201807057U (en) * | 2009-07-22 | 2011-04-27 | 李雁鹏 | Design and application of novel experimental equipment for sleep deprivation of rat |
| CN102451046A (en) * | 2010-10-25 | 2012-05-16 | 陈浩杰 | Fixing device for vena caudalis injection of mouse |
| CN202010217U (en) * | 2011-03-24 | 2011-10-19 | 宋小莉 | Mouse fixing device |
| CN202051849U (en) * | 2011-05-13 | 2011-11-30 | 中国人民解放军第四军医大学 | Lab mouse tail intravenous injection fixing device |
| CN202892144U (en) * | 2012-11-29 | 2013-04-24 | 中国人民解放军第三军医大学第一附属医院 | Inflatable rat fixer |
| CN203576689U (en) * | 2013-11-25 | 2014-05-07 | 温州医科大学附属第一医院 | Device special for rat intraperitoneal injection |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958119A (en) * | 2015-07-24 | 2015-10-07 | 温州医科大学附属第一医院 | Acupuncture treatment fixator providing convenience for fixing mouse |
| CN106725980A (en) * | 2016-12-30 | 2017-05-31 | 谷翠芝 | Rat Thoracicoabdominal puncture fixing device |
| CN109124810A (en) * | 2018-09-26 | 2019-01-04 | 华蓥市铜堡初级中学 | A kind of animal blood taking platform |
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| Publication number | Publication date |
|---|---|
| CN103654996B (en) | 2015-08-05 |
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| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhuang Chengle Inventor after: Yu Zhen Inventor after: Zheng Beishi Inventor after: Chen Weizhe Inventor after: Chen Bicheng Inventor before: Yu Zhen Inventor before: Zhuang Chengle Inventor before: Zheng Beishi Inventor before: Chen Weizhe Inventor before: Chen Bicheng |
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Free format text: CORRECT: INVENTOR; FROM: YU ZHEN ZHUANG CHENGLE ZHENG BEISHI CHEN WEIZHE CHEN BICHENG TO: ZHUANG CHENGLE YU ZHEN ZHENG BEISHI CHEN WEIZHE CHEN BICHENG |
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| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhuang Chengle Inventor after: Huang Dongdong Inventor after: Zheng Beishi Inventor after: Yu Zhen Inventor after: Chen Fanfeng Inventor after: Zhou Chongjun Inventor after: Chen Weizhe Inventor after: Chen Bicheng Inventor after: Shen Xian Inventor before: Zhuang Chengle Inventor before: Yu Zhen Inventor before: Zheng Beishi Inventor before: Chen Weizhe Inventor before: Chen Bicheng |
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Free format text: CORRECT: INVENTOR; FROM: ZHUANG CHENGLE YU ZHEN ZHENG BEISHI CHEN WEIZHE CHEN BICHENG TO: ZHUANG CHENGLE HUANG DONGDONG ZHENG BEISHI YU ZHEN CHEN FANFENG ZHOU CHONGJUN CHEN WEIZHE CHEN BICHENG SHEN XIAN |
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Granted publication date: 20150805 Termination date: 20161125 |
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