CN103645176A - Detection method for pesticide residues on surfaces of vegetables and fruits and corresponding detection device - Google Patents
Detection method for pesticide residues on surfaces of vegetables and fruits and corresponding detection device Download PDFInfo
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- CN103645176A CN103645176A CN201310652056.0A CN201310652056A CN103645176A CN 103645176 A CN103645176 A CN 103645176A CN 201310652056 A CN201310652056 A CN 201310652056A CN 103645176 A CN103645176 A CN 103645176A
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Abstract
The invention provides a detection method for pesticide residues on surfaces of vegetables and fruits. The detection method comprises the following steps: step A, pre-treating a sample to be detected to prepare a raw solution to be detected; standing the raw solution to be detected for more than three minutes for later use; B, taking the raw solution to be detected and adding an ion reagent for enriching and extracting the raw solution to be detected; C, stirring by a glass rod in the extracting process and standing; taking supernatant liquid; D, adding an oxidant into the supernatant liquid to generate photochemical reaction; E, converting an optical signal of the photochemical reaction into reaction data taking an electric signal as a carrier by using a photochemical gain circuit; and F, analyzing and comparing the reaction data and sample data stored in a database by a processor, and outputting a comparing result. Meanwhile, the invention further provides a detection device for the detection method; the detection device comprises a memorizer 1, the processor 2, the photochemical gain circuit 3, an amplification circuit 4 and a display circuit 5. According to the detection method and the detection device, a detection manner is convenient and rapid and a detection result is generated by a computer; an inspector can rapidly judge according to the result.
Description
Technical field
The present invention relates to detection method and the corresponding pick-up unit of a kind of vegetables and fruits surface residues of pesticides.
Background technology
Food security is just causing the strong interest of the whole society, and what be closely related with people's daily life is that Detecting Pesticide on the agricultural product such as vegetables and fruits becomes important step wherein.Conventional detection scheme is that sample is delivered to laboratory, tries standby, reagent and its professional knowledge judge and obtain assay by professional reviewer by check.Like this, not only need reviewer need there is certain patent knowledge, and checkout procedure consumption is long, efficiency is low.
Luminol (chemical formula is C8H7N3O2), has another name called luminol.Under normal temperature, being a kind of yellow crystals or ecru powder, is a kind of more stable artificial synthetic organic compound, adds hydrogen peroxide to can be made into detection reagent.Through realizing, find, the decomposition of residues of pesticides energy catalyzing hydrogen peroxide, allows hydrogen peroxide become water and single oxygen, and it is luminous by it that single oxygen reoxidizes luminol, according to photochemically reactive light signal strength, can judge its residues of pesticides grade.
Summary of the invention
Content based on background technology, the present invention proposes the detection method of a kind of vegetables and fruits surface residues of pesticides, and its detection mode is convenient, fast and generate testing result by computing machine, and reviewer can judge rapidly according to result.Its technical scheme is as follows:
A detection method for vegetables and fruits surface residues of pesticides, comprises the steps:
The pre-service of A, sample to be checked, described pre-service comprises, to greengrocery get respectively its broad-leaved part and stem or to fruit after its epidermis, shred and add pure water to form stoste to be checked, and this stoste to be checked is standing more than 3 minutes, standby;
B, get above-mentioned stoste to be checked, add Ion reagent to carry out enrichment and extraction to it, described Ion reagent is normal-butyl-3-formaldehyde imidazoles tetrafluoro;
C, said extracted process are standing after stirring with glass bar, get supernatant liquor;
D, in above-mentioned clear liquid, add oxygenant there is photochemical reaction;
E, utilize optical gain circuit, photochemically reactive light signal is converted to and take the response data that electric signal is carrier;
F, processor perform an analysis the sample data of storing in response data and database, compare, and export comparison result;
The form of described comparison result can be the residues of pesticides grade with color grading, or residues of pesticides numerical range, or other standard sign.
Particularly, the ratio of described sample to be checked and pure water is 1:5.
Particularly, the ratio of described source to be checked liquid and Ion reagent is 1:6.
Particularly, described oxygenant is the oxalate of luminol-hydrogen peroxide or hydrogen peroxide oxidation aromatic hydrocarbon; The photochemical reaction mechanism of described oxalate is to be accompanied by chemical reaction to produce high-octane fluorescent core 1,2-cyclohexane diketone also discharges an electronics to intermediate, this electronics makes it in excited state while going back to fluorescent core, produces chemiluminescence when excited state is got back to ground state.
The beneficial effect of such scheme is: reviewer only need make simple chemical reaction and prepare, and the final sample data by computing machine and standard test gained to photochemically reactive judgement compares, and directly generates judged result.So just needn't the rigid checker of requirement there is professional knowledge really up to the mark, only need know the addition of detecting step and reagent.
The present invention also proposes the pick-up unit based on above-mentioned detection method, and it comprises storer, processor, optical gain circuit, amplifying circuit, display circuit;
Described storer, stores for the response data of comparing and the residues of pesticides class letter corresponding with this response data;
Described optical gain circuit, for obtaining the photochemically reactive light signal of luminol, and is converted to photochemically reactive light signal to take the response data that electric signal is carrier; This response data is sent to processor through amplifying circuit;
Described processor, performs an analysis, compares for the sample data that response data and database are stored, and control described display circuit and export corresponding residues of pesticides class letter.
Such scheme also comprises:
Further, described photomultiplier transit benefit circuit comprises by chip U1, resistance R 1 and slide rheostat Rz;
Described chip comprises light sensation unit, for receiving optical signals and according to light intensity, is converted to corresponding electric current, realizes opto-electronic conversion; Described chip is provided with feedback end Vref, voltage controling end Vcont, described resistance R 1 and slide rheostat Rz are connected in series to described feedback end Vref and with reference between ground, voltage controling end Vcont described in the slip termination of rheostat Rz, for regulating the opto-electronic conversion voltage of chip U1.
The beneficial effect of such scheme is: computing machine can be made judgement accurately according to comparison result, there will not be artificial judgement mistake.And only need enough detailed sample datas of typing, just can improve the precision of detection.
Accompanying drawing explanation
Fig. 1 is detection method process flow diagram.
Fig. 2 is structure of the detecting device schematic diagram.
Fig. 3 is optical gain circuit diagram.
Embodiment
As follows by reference to the accompanying drawings, technical scheme of the present invention is described:
As shown in Figure 1, the detection method of a kind of vegetables and fruits surface residues of pesticides, comprises the steps:
The pre-service of S01, sample to be checked, described pre-service comprises, to greengrocery get respectively its broad-leaved part and stem or to fruit chopping be that 1:5 adds pure water to form stoste to be checked in the ratio of sample to be checked and pure water after its epidermis, and this stoste to be checked is standing more than 3 minutes, standby;
S02, getting above-mentioned stoste to be checked, is that 1:6 adds Ion reagent to carry out enrichment and extraction to it in the ratio of source to be checked liquid and Ion reagent, and described Ion reagent is normal-butyl-3-formaldehyde imidazoles tetrafluoro;
S03, said extracted process are standing after stirring with glass bar, get supernatant liquor;
S04, in above-mentioned clear liquid, add luminol-hydrogen peroxide agent there is photochemical reaction;
S05, utilize optical gain circuit, photochemically reactive light signal is converted to and take the response data that electric signal is carrier;
S06, processor perform an analysis the sample data of storing in response data and database, compare, and export comparison result;
The form of described comparison result can be the residues of pesticides grade with color grading, or residues of pesticides numerical range, or other standard sign.
As shown in Figure 2, the pick-up unit of a kind of vegetables and fruits surface residues of pesticides, it comprises storer 1, processor 2, optical gain circuit 3, amplifying circuit 4, display circuit 5;
Described storer 1, stores for the response data of comparing and the residues of pesticides class letter corresponding with this response data;
Described optical gain circuit 3, for obtaining the photochemically reactive light signal of luminol, and is converted to photochemically reactive light signal to take the response data that electric signal is carrier; This response data is sent to processor 2 through amplifying circuit 4;
Described processor 2, performs an analysis, compares for the sample data that response data and database are stored, and controls residues of pesticides class letter corresponding to described display circuit 5 output.
As shown in Figure 3, described photomultiplier transit benefit circuit 3 comprises by chip U1, resistance R 1 and slide rheostat Rz;
Described chip U1 comprises light sensation unit 301, for receiving optical signals and according to light intensity, is converted to corresponding electric current, realizes opto-electronic conversion; Described chip is provided with feedback end Vref, voltage controling end Vcont, described resistance R 1 and slide rheostat Rz are connected in series to described feedback end Vref and with reference between ground, voltage controling end Vcont described in the slip termination of rheostat Rz, for regulating the opto-electronic conversion voltage of chip U1.
Above-mentionedly should be understood to illustrating of embodiment of the present invention, technical scheme all and of the present invention technology identical, approximate or that done is based on this deduced, is replaced, and all should be considered as protection scope of the present invention.
Claims (6)
1. a detection method for vegetables and fruits surface residues of pesticides, is characterized in that comprising the steps:
The pre-service of A, sample to be checked, described pre-service comprises, to greengrocery get respectively its broad-leaved part and stem or to fruit after its epidermis, shred and add pure water to form stoste to be checked, and this stoste to be checked is standing more than 3 minutes, standby;
B, get above-mentioned stoste to be checked, add Ion reagent to carry out enrichment and extraction to it, described Ion reagent is normal-butyl-3-formaldehyde imidazoles tetrafluoro;
C, said extracted process are standing after stirring with glass bar, get supernatant liquor;
D, in above-mentioned clear liquid, add oxygenant there is photochemical reaction;
E, utilize optical gain circuit, photochemically reactive light signal is converted to and take the response data that electric signal is carrier;
F, processor perform an analysis the sample data of storing in response data and database, compare, and export comparison result;
The form of described comparison result can be the residues of pesticides grade with color grading, or residues of pesticides numerical range, or other standard sign.
2. the detection method of vegetables and fruits according to claim 1 surface residues of pesticides, is characterized in that: the ratio of described sample to be checked and pure water is 1:5.
3. the detection method of vegetables and fruits according to claim 1 surface residues of pesticides, is characterized in that: the ratio of described source to be checked liquid and Ion reagent is 1:6.
4. the detection method of vegetables and fruits according to claim 1 surface residues of pesticides, is characterized in that: described oxygenant is the oxalate of luminol-hydrogen peroxide or hydrogen peroxide oxidation aromatic hydrocarbon.
5. for a pick-up unit for the detection method of vegetables and fruits surface residues of pesticides described in claim 1, it is characterized in that: comprise storer, processor, optical gain circuit, amplifying circuit, display circuit;
Described storer, stores for the response data of comparing and the residues of pesticides class letter corresponding with this response data;
Described optical gain circuit, for obtaining the photochemically reactive light signal of luminol, and is converted to photochemically reactive light signal to take the response data that electric signal is carrier; This response data is sent to processor through amplifying circuit;
Described processor, performs an analysis, compares for the sample data that response data and database are stored, and control described display circuit and export corresponding residues of pesticides class letter.
6. pick-up unit according to claim 5, is characterized in that: described photomultiplier circuit comprises by chip U1, resistance R 1 and slide rheostat Rz;
Described chip comprises light sensation unit, for receiving optical signals and according to light intensity, is converted to corresponding electric current, realizes opto-electronic conversion; Described chip is provided with feedback end Vref, voltage controling end Vcont, described resistance R 1 and slide rheostat Rz are connected in series to described feedback end Vref and with reference between ground, voltage controling end Vcont described in the slip termination of rheostat Rz, for regulating the opto-electronic conversion voltage of chip U1.
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| CN201310652056.0A CN103645176A (en) | 2013-12-03 | 2013-12-03 | Detection method for pesticide residues on surfaces of vegetables and fruits and corresponding detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113340685A (en) * | 2021-05-10 | 2021-09-03 | 阳江职业技术学院 | Agricultural and sideline product pesticide residue comprehensive detection device and method and health risk assessment method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426167A (en) * | 2011-09-10 | 2012-04-25 | 常熟理工学院 | Photoelectric conversion based rapid detection method for pesticide residual toxicity |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102426167A (en) * | 2011-09-10 | 2012-04-25 | 常熟理工学院 | Photoelectric conversion based rapid detection method for pesticide residual toxicity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113340685A (en) * | 2021-05-10 | 2021-09-03 | 阳江职业技术学院 | Agricultural and sideline product pesticide residue comprehensive detection device and method and health risk assessment method |
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Application publication date: 20140319 |