CN115372328B

CN115372328B - Lithium ion measurement method and device

Info

Publication number: CN115372328B
Application number: CN202211025624.XA
Authority: CN
Inventors: 刘景堂
Original assignee: Micas Biotechnology Suzhou Co ltd
Current assignee: Micas Biotechnology Suzhou Co ltd
Priority date: 2022-08-25
Filing date: 2022-08-25
Publication date: 2023-10-24
Anticipated expiration: 2042-08-25
Also published as: CN115372328A

Abstract

Embodiments of the present disclosure provide a lithium ion measurement method and apparatus, the method comprising sampling a sampleAdding the solution into 1, 4-dihydroxyanthraquinone, organic solvent and strong alkali solution, mixing to obtain solution to be tested, wherein the structural formula of the 1, 4-dihydroxyanthraquinone isThe method comprises the steps of carrying out a first treatment on the surface of the Illuminating the solution to be detected by using detection light to obtain the fluorescence intensity of the solution to be detected after illumination; the lithium ion concentration in the sample solution is determined based on the fluorescence intensity. The lithium ion measurement method and the device can conveniently and accurately detect lithium ions in the solution.

Description

Lithium ion measurement method and device

Technical Field

The present disclosure relates to the field of ion concentration detection technologies, and in particular, to a method and an apparatus for measuring lithium ions.

Background

Detection of lithium ion concentration is important in many fields. For example, lithium carbonate is commonly used as a drug for treating bipolar disorder, but the concentration of lithium ions in blood exceeds a certain amount to cause poisoning of human body, so that the concentration of lithium ions in saliva and blood of human body needs to be detected regularly after the drug is used to ensure that the concentration of lithium ions does not exceed the safe use range. Also for example, in field surveys and industrial processes, rapid detection of lithium ion concentration in aqueous solutions plays an important role in surveys and production.

It is therefore desirable to provide a lithium ion measurement method and apparatus that allows for convenient, low cost detection of lithium ion concentration in a solution.

Disclosure of Invention

In order to solve the technical problems of complex detection and high cost of lithium ions and difficult movement of detection equipment, one or more embodiments of the present disclosure provide a method and an apparatus for measuring lithium ions, where the method includes: adding the sample solution into 1, 4-dihydroxyanthraquinone, organic solvent and strong alkali solution, mixing to obtain solution to be tested, wherein the structural formula of the 1, 4-dihydroxyanthraquinone isIlluminating the solution to be detected by using detection light to obtain the fluorescence intensity of the solution to be detected after illumination; the lithium ion concentration in the sample solution is determined based on the fluorescence intensity.

In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone in the test solution is between 10 and 100. Mu. Mol/L.

In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone in the test solution is between 10 and 40. Mu. Mol/L.

In some embodiments, the strong base comprises NaOH, and the concentration of NaOH in the solution to be tested is 1.2-5 mmol/L.

In some embodiments, the concentration of NaOH in the solution to be tested is 1.2-3.5 mmol/L.

In some embodiments, the wavelength of the detection light covers 560-600nm.

In some embodiments, determining the concentration of lithium ions in the sample solution based on the fluorescence intensity comprises: and determining the lithium ion concentration in the sample solution based on a preset linear relation between the fluorescence intensity and the lithium ion concentration.

In some embodiments, determining the concentration of lithium ions in the sample solution based on the fluorescence intensity comprises: and determining the concentration of lithium ions in the sample solution based on the treatment of the fluorescence intensity by the determination model, wherein the determination model is a machine learning model.

One or more embodiments of the present disclosure provide a portable device for lithium ion measurement, the portable device including an LED light source, a photodetector, a structural member, and an electronic component, wherein the wavelength of the LED light source is 560-600nm, the photodetector is used to obtain a fluorescence intensity of the light source after the light source irradiates a sample solution, and the fluorescence intensity is used to determine a lithium ion solubility in the sample solution.

In some embodiments, the lithium ion measurement portable device further comprises: the intensity integral of light in the wavelength range 560-600nm has a ratio of more than a preset value in the total intensity integral in the LED light source.

Drawings

The present specification will be further elucidated by way of example embodiments, which will be described in detail by means of the accompanying drawings. The embodiments are not limiting, in which like numerals represent like structures, wherein:

FIG. 1 is a schematic illustration of a method of lithium ion measurement as shown in some embodiments of the present disclosure;

FIG. 2 is a reaction equation for generating a fluorescent complex according to some embodiments of the present disclosure;

FIG. 3 is a graph showing fluorescence emission spectra of solutions to be tested incorporating different reagents according to some embodiments of the present disclosure;

FIG. 4 is a graph of fluorescence intensity for test solutions containing different concentrations of 1, 4-dihydroxyanthraquinone at different NaOH concentrations according to some embodiments of the present disclosure;

FIG. 5 is a graph of fluorescence intensity for test solutions containing NaOH at different concentrations for different concentrations of 1, 4-dihydroxyanthraquinone according to some embodiments of the present disclosure;

FIG. 6 is a standard curve and correlation of the two obtained by testing a solution to be tested according to various apparatus shown in some embodiments of the present disclosure;

FIG. 7 is a standard graph of saliva shown according to some embodiments of the present description;

fig. 8 is a schematic structural view of a lithium ion measurement portable device according to some embodiments of the present description.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present specification, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is apparent that the drawings in the following description are only some examples or embodiments of the present specification, and it is possible for those of ordinary skill in the art to apply the present specification to other similar situations according to the drawings without inventive effort. Unless otherwise apparent from the context of the language or otherwise specified, like reference numerals in the figures refer to like structures or operations.

The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, were purchased from conventional Biochemical reagent companies.

Lithium has wide application in the fields of chemical industry and medicine. In the industrial production process, lithium ions possibly enter the water environment along with industrial wastewater, and the lithium ion measurement method and the device provided by the specification can be used for detecting trace lithium ions in the drinking water. Lithium is often used in the medical field to treat bipolar affective disorders, so the lithium ion measurement method and device provided in the present specification can also be used to detect the concentration of lithium ions in blood, saliva, etc. in a human body.

FIG. 1 is a diagram of some implementations of the present disclosureA schematic diagram of a lithium ion measurement method is shown in the example. As shown in fig. 1, the lithium ion measurement method provided in the present specification specifically includes the following steps: step 110, adding the sample solution into 1, 4-dihydroxyanthraquinone, organic solvent and alkali solution, mixing to obtain the solution to be tested, wherein the structural formula of the 1, 4-dihydroxyanthraquinone isStep 120, irradiating the solution to be measured with detection light to obtain the fluorescence intensity of the solution to be measured after irradiation; step 130, determining the lithium ion concentration in the sample solution based on the fluorescence intensity.

The concentration of 1, 4-dihydroxyanthraquinone in the solution to be tested is shown in FIG. 4 and related content, and the concentration of the strong base solution is shown in FIG. 5 and related content.

The sample solution is a solution that may contain lithium ions. In some embodiments, the sample solution comprises an aqueous solution, a blood, saliva, urine, or the like solution.

In some embodiments, the organic solvent may include dimethyl sulfoxide, ethanol, acetone, and the like. The organic solvent is added to increase the solubility of the 1, 4-dihydroxyanthraquinone in the solution to be measured, which is favorable for the chemical reaction of the 1, 4-dihydroxyanthraquinone and lithium ions.

In some embodiments, the strong base may include NaOH, KOH, and the like. Under alkaline condition, phenolic hydroxyl of 1, 4-dihydroxyanthraquinone and NaOH generate phenolate, the phenolate reacts with lithium ion to generate fluorescent compound, the fluorescent compound generates fluorescence under the irradiation of detection light, and the reaction equation and the structural formula of the fluorescent compound are shown in figure 2.

In some embodiments, the detection light irradiates the fluorescent compound in the solution to be detected to generate fluorescence, and the fluorescence intensity of the solution to be detected after irradiation can be obtained through a photoelectric detector. See fig. 8 and related description for more details regarding photodetectors.

In some embodiments, the lithium ion concentration in the sample solution may be determined based on the fluorescence intensity by a determination model. In some embodiments, the concentration of lithium ions in the sample solution may be determined based on a linear relationship between fluorescence intensity and lithium ion concentration. See fig. 7 and related description for more details regarding the linear relationship between fluorescence intensity and lithium ion concentration.

FIG. 3 is a graph showing fluorescence emission spectra of solutions to be tested to which different reagents have been added in some embodiments of the present disclosure. As shown in fig. 3, (a) corresponds to a solution obtained by adding 1, 4-dihydroxyanthraquinone and NaOH to a solution containing lithium ions, (b) corresponds to a solution obtained by adding 1, 4-dihydroxyanthraquinone and NaOH to a solution not containing lithium ions, and (c) corresponds to a solution obtained by adding 1, 4-dihydroxyanthraquinone to a solution not containing lithium ions. Wherein, the organic solvent dimethyl sulfoxide is added into the solutions of the figures (a), (b) and (c). The observation shows that (a) curve has obvious fluorescence emission peak at 625nm, and (b) curve and (c) curve have no fluorescence emission peak at 625nm, which indicates that only 1, 4-dihydroxyanthraquinone, lithium ion and NaOH coexist in the solution to be tested, can react to generate fluorescent compound to emit fluorescence.

In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone is in the range of 10 to 100. Mu. Mol/L.

FIG. 4 is a graph of fluorescence intensity corresponding to a test solution containing 1, 4-dihydroxyanthraquinone at different concentrations of NaOH according to some embodiments of the present disclosure, with fluorescence intensity on the ordinate and 1, 4-dihydroxyanthraquinone concentration on the abscissa, for detecting the effect of 1, 4-dihydroxyanthraquinone at different concentrations on the fluorescence intensity of the test solution, to select suitable concentrations of 1, 4-dihydroxyanthraquinone. As shown in FIG. 4, when the concentration of NaOH in the solution to be detected is 1.2mmol/L, 2.5mmol/L, 3.3mmol/L and 5.0mmol/L, respectively, the fluorescence intensity corresponding to 1, 4-dihydroxyanthraquinone with the concentration of 10-100 mu mol/L is selected. The analysis result shows that the higher the concentration of 1, 4-dihydroxyanthraquinone, the higher the fluorescence intensity within a certain range. Wherein, when the concentration of the 1, 4-dihydroxyanthraquinone is 10-100 mu mol/L, the fluorescence intensity is obvious, which is beneficial to observation.

In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone is in the range of 10 to 40. Mu. Mol/L.

In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone may also be in the range of 10 to 70. Mu. Mol/L. In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone may also be in the range of 10 to 60. Mu. Mol/L. In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone may also be in the range of 10 to 50. Mu. Mol/L. In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone may also be 20 to 60. Mu. Mol/L. In some embodiments, the concentration of 1, 4-dihydroxyanthraquinone may also be 20 to 40. Mu. Mol/L. Further analysis of FIG. 4 shows that the greater the concentration of 1, 4-dihydroxyanthraquinone, the smaller the increase in fluorescence intensity, indicating that an increase in the concentration of 1, 4-dihydroxyanthraquinone may decrease the sensitivity of fluorescence emission. Too high a concentration of 1, 4-dihydroxyanthraquinone may absorb some of the fluorescence, thereby blocking the fluorescence emission. Thus, in some embodiments, the slope of the fluorescence emission curve is selected to increase the concentration of the 1, 4-dihydroxyanthraquinone corresponding to a greater portion, thereby selecting a concentration of 1, 4-dihydroxyanthraquinone of 10-40. Mu. Mol/L. The sensitivity of fluorescence detection is high, so that the detection of the lithium ion concentration is more accurate.

FIG. 5 is a graph of fluorescence intensity corresponding to a test solution containing NaOH of different concentrations at different concentrations of 1, 4-dihydroxyanthraquinone according to some embodiments of the present disclosure, where the ordinate is fluorescence intensity and the abscissa is concentration of NaOH, and the graph is used to detect the effect of NaOH of different concentrations in the test solution on fluorescence intensity, so as to select the applicable concentration of NaOH. As shown in FIG. 5, when the concentration of 1, 4-dihydroxyanthraquinone in the solution to be detected is 100 mu mol/L, 50 mu mol/L, 25 mu mol/L and 12 mu mol/L, respectively, the concentration of NaOH in the solution to be detected is 1.2-5 mmol/L, and the fluorescence intensity is corresponding. The analysis result shows that when the concentration of NaOH is 1.2-5 mmol/L, the fluorescence intensity is obvious, and the fluorescence intensity can be used as the concentration range of NaOH in the solution to be measured.

In some embodiments, the concentration of NaOH in the solution to be tested is 1.2-3.5 mmol/L. In some embodiments, the concentration of NaOH in the solution to be tested is 1.5-4 mmol/L. In some embodiments, the concentration of NaOH in the solution to be tested may also be 2-3.5 mmol/L. In some embodiments, the concentration of NaOH in the solution to be tested may also be 3-3.5 mmol/L.

Further analysis of FIG. 5 shows that as the concentration of NaOH increases, the fluorescence intensity of the solution to be measured decreases, so that too high concentration of NaOH is unfavorable for fluorescence emission of the solution to be measured, and the observation shows that the fluorescence intensity of the solution to be measured reaches a peak value when the concentration of NaOH is 3.5mmol/L, so that the concentration of NaOH in the solution to be measured is selected to be 1.2-3.5 mmol/L.

FIG. 6 is a standard curve and correlation of the two obtained by testing solutions to be tested according to various devices shown in some embodiments of the present disclosure. As shown in fig. 6, fig. 6 (a) shows a standard curve obtained by detecting a solution to be measured using a spectrophotometer, fig. 6 (b) shows a standard curve obtained by detecting a solution to be measured using a lithium ion measuring portable device, and fig. 6 (c) shows a correlation between a standard curve obtained by detecting a solution to be measured using a spectrophotometer and a standard curve obtained by detecting a solution to be measured using a lithium ion measuring portable device.

In some embodiments, the step of detecting a standard curve derived from the solution to be tested using a spectrophotometer comprises: adding 10 mu L of solution containing different lithium ion concentrations and 40 mu L of NaOH solution into 1mL of dimethyl sulfoxide solution containing 1, 4-dihydroxyanthraquinone, and mixing to obtain a plurality of groups of solutions to be tested corresponding to the lithium ion concentrations, wherein the lithium ion concentrations are respectively 0 mu mol/L, 2 mu mol/L, 5 mu mol/L, 20 mu mol/L, 30 mu mol/L and 40 mu mol/L, the NaOH concentration in the solutions to be tested is 1.4mmol/L, and the 1, 4-dihydroxyanthraquinone concentration is 15 mu mol/L; the solution to be detected is excited by using detection light with the wavelength of 602nm to obtain a plurality of groups of fluorescence emission spectrums, the fluorescence intensity corresponding to the emission peak 625nm is selected, the lithium ion concentration is taken as an abscissa, and the fluorescence intensity is taken as an ordinate to obtain a standard curve y= 3.5843x-10.252 shown in fig. 6 (a). The equation obtains a coefficient of 3.5843 and an intercept of-10.252 by linearly fitting coordinate points corresponding to 6 different lithium ion concentrations, wherein x represents the lithium ion concentration and y represents the fluorescence intensity.

In some embodiments, the step of detecting a standard curve derived from a solution to be measured using a lithium ion measurement portable device comprises: adding 10 mu L of solution containing different lithium ion concentrations and 40 mu L of NaOH solution into 1mL of dimethyl sulfoxide solution containing 1, 4-dihydroxyanthraquinone, and mixing to obtain a plurality of groups of solutions to be tested corresponding to the lithium ion concentrations, wherein the lithium ion concentrations are respectively 0 mu mol/L, 2 mu mol/L, 5 mu mol/L, 20 mu mol/L, 30 mu mol/L and 40 mu mol/L, the NaOH concentration in the solutions to be tested is 1.4mmol/L, and the 1, 4-dihydroxyanthraquinone concentration is 15 mu mol/L; and exciting the solution to be detected by using detection light of the lithium ion measurement portable device to obtain a plurality of groups of fluorescence emission spectrums, wherein the wavelength of the detection light is 560-600nm. A standard curve y=0.850 x+12.20 as shown in fig. 6 (b) was obtained in a manner as shown in fig. 6 (a).

As can be seen from comparison of fig. 6 (a) and fig. 6 (b), when the lithium ion concentration is lower than 5 μmol/L, the linearity of detecting the solution to be measured using the lithium ion measuring portable device is better, which indicates that the detection light in the portable device is a wavelength covering 560-600nm instead of a single wavelength, and the accuracy of detecting the low lithium ion concentration by the lithium ion measuring portable device can be improved.

As shown in fig. 6 (c), the fluorescence intensity measured by the spectrophotometer is on the ordinate, and the fluorescence intensity measured by the lithium ion measuring portable device is on the abscissa, for checking the correlation of the two. For example, when the lithium ion concentration is 0. Mu. Mol/L, the fluorescence intensity measured by the spectrophotometer is 2.995, and the fluorescence intensity measured by the portable device is 12.67, corresponding to the coordinate point (12.67,2.995). Similarly, 10 coordinate points with the lithium ion concentration of 0 mu mol/L, 2 mu mol/L, 5 mu mol/L, 10 mu mol/L, 20 mu mol/L, 30 mu mol/L, 40 mu mol/L, 50 mu mol/L, 55 mu mol/L and 60 mu mol/L are selected and respectively corresponding to (12.67,2.995), (14.00,4.945), (15.67,9.328), (20.33, 22.893), (29.33, 59.941), (39.00, 102.554), (45.33, 130.379), (53.33, 176.443), (57.00, 195.880), (59.00 and 206.412) to perform linear fitting to obtain a curve y= 4.4266x-61.835. The result shows that the correlation between the portable device and the spectrophotometer is 0.9931, and the high correlation proves that the portable device for measuring lithium ions can accurately detect the concentration of lithium ions in a sample solution, and the portable device is convenient for mobile detection, so that the problem that equipment is fixed and inconvenient to detect is avoided.

In some embodiments, the lithium ion concentration in the sample solution may be determined based on a preset linear relationship of fluorescence intensity and lithium ion concentration. In some embodiments, the linear relationship includes a positive correlation of fluorescence intensity corresponding to lithium ion concentration and lithium ion concentration. For example, by obtaining a standard curve y=0.850 x+12.20 in fig. 6 (b), it is known that the fluorescence intensity and the lithium ion concentration are in a linear relationship of positive correlation, and the lithium ion concentration in the sample solution is determined. In the test process, 10 mu L of sample solution is added into 1.04ml of dimethyl sulfoxide solution containing 1.4mmol/L NaOH and 15 mu mol/L1, 4-dihydroxyanthraquinone, the mixture is mixed to obtain a solution to be tested, the solution to be tested is irradiated by detection light with the wavelength of 560-600nm to obtain fluorescence intensity Y, and then the fluorescence intensity Y is substituted into a standard curve y=0.850x+12.20 as Y value, so that the x value can be obtained, and the lithium ion concentration in the sample solution is obtained.

In some embodiments, the lithium ion concentration in the saliva solution may be detected. And adding 35 mu L of saliva, 5 mu L of lithium ion solution with different concentrations and 8 mu L of NaOH solution into 1ml of dimethyl sulfoxide solution containing 15 mu mol/L1, 4-dihydroxyanthraquinone, mixing to obtain a solution to be tested, wherein the concentration of NaOH in the solution to be tested is 1.4mmol/L, and the solution to be tested is irradiated by detection light with the wavelength of 560-600nm, so that the fluorescence intensities corresponding to the lithium ion solutions with different concentrations can be obtained, wherein the fluorescence intensities corresponding to the lithium ion concentrations are 16, 17, 18, 19, 24, and 46 are respectively selected, and linear curve fitting is carried out according to the linear curve of (10, 16), (20, 17), (30, 18), (40, 19), (75, 24), (34), (7+46) as shown in figure 7+10.12α+12x=7. Knowing the standard curve, the lithium ion concentration in saliva can be determined in a similar manner as above.

In some embodiments, the lithium ion concentration of a solution of blood, urine, etc. may also be determined based on a linear relationship of fluorescence intensity to lithium ion concentration.

In some embodiments, determining the concentration of lithium ions in the sample solution based on the fluorescence intensity may include determining the concentration of lithium ions in the sample solution based on a treatment of the fluorescence intensity by the determination model.

The determination model may be a model for determining the concentration of lithium ions in the sample solution. In some embodiments, the determined model is a machine learning model. For example, convolutional neural networks (Convolutional Neural Networks, CNN), deep neural networks (Deep Neural Networks, DNN), or a combination thereof.

In some embodiments, the determination model may process the fluorescence intensity to determine the concentration of lithium ions in the sample solution. The input of the determination model can comprise fluorescence intensity, sample solution type, organic solvent type, concentration of 1, 4-dihydroxyanthraquinone, type and concentration of strong base, wavelength of detection light, and the output can comprise lithium ion concentration in the sample solution. The fluorescence intensity is generated when the kind of the sample solution to be input, the kind of the organic solvent, the concentration of 1, 4-dihydroxyanthraquinone, the kind and concentration of the strong base, and the wavelength of the detection light are determined. For example, 1.04ml of a dimethyl sulfoxide solution containing 1.4mmol/L NaOH and 15. Mu. Mol/L1, 4-dihydroxyanthraquinone is added to the aqueous solution, and the concentration of lithium ions in the aqueous solution is outputted when the fluorescence intensity obtained by irradiation with detection light having a wavelength of 560 to 600nm is used.

The deterministic model may be obtained through training. In some embodiments, the training sample may include several sets of training data, each set of training data including a sample fluorescence intensity of the sample solution to be tested, and a sample type of the sample solution in the sample solution to be tested, a sample type of the organic solvent, a sample concentration of the 1, 4-dihydroxyanthraquinone, a sample type and sample concentration of the strong base, a sample detection light wavelength, and the label of the training sample may be a lithium ion concentration in the sample solution to be tested. Inputting the training sample into an initial determination model, constructing a loss function based on the output of the initial determination model and the label, and iteratively updating parameters of the initial determination model based on the loss function until the training is finished and a trained determination model is obtained when the preset condition is met. The preset conditions may include, but are not limited to, the loss function converging, the training period reaching a threshold, etc.

In some embodiments of the present disclosure, by processing the fluorescence intensity using a determination model to determine the lithium ion concentration in the sample solution, the relationship between the fluorescence intensity and the lithium ion concentration can be more accurately fitted, and the detection accuracy of the lithium ion concentration is effectively improved.

As shown in fig. 8, in some embodiments, a lithium ion measurement portable device may include an LED light source 1, a photodetector 2, a structural member 3, and an electronic component 4.

The LED light source 1 may be used to illuminate the solution to be measured to excite fluorescence. The LED light source can emit light with different colors, and the different light emitting colors correspond to a certain wavelength range. For example, when the light emission color of the LED light source is red, the wavelength range is 615-650 nm; when the luminous color of the LED light source is orange, the wavelength range is 600-610 nm; when the light emission color of the LED light source is yellow, the wavelength range is 580-595 nm.

In some embodiments, the wavelength coverage of the LED light source is 560-600nm. In some embodiments, the integral of the intensity of light in the wavelength range 560-600nm is greater than a preset value in the total integral of the intensity in the LED light source. The preset value may be 95%, 90%, etc. Significant fluorescence intensity can be advantageously stimulated by having the integral of the intensity of light in the wavelength range 560-600nm occupy a ratio of greater than a preset value in the total integral of the intensity in the LED light source. For example, when the intensity integral of light in the wavelength range of 560-600nm accounts for more than 90% of the total intensity integral in the LED light source, the LED light source irradiates the fluorescence intensity excited by the solution to be measured, which is favorable for observation. The LED light source provides excitation light ranging from 560-600nm, so that the total excitation energy is greatly increased over a single wavelength, thereby enhancing the fluorescence intensity of the emitted light.

The photodetector 2 may be used to sense the fluorescence intensity of the solution to be measured after it has been irradiated. In some embodiments, the photodetector is used to obtain the fluorescence intensity of the light source after irradiating the solution to be measured, and the fluorescence intensity is used to determine the lithium ion solubility in the sample solution. For example, the LED light source 1 in the lithium ion measuring portable device emits fluorescence after irradiating the solution to be measured prepared from the sample solution, the photodetector 2 obtains the fluorescence intensity, and then the solubility of lithium ions in the sample solution is determined according to the determination model or the linear relationship between the fluorescence intensity and the lithium ion concentration.

The photodetector 2 can receive and detect a spectrum in a range of 300-1000nm, and can cover a wavelength range of fluorescence emission of a fluorescent compound generated by the 1, 4-dihydroxyanthraquinone and lithium ions, so that the fluorescence intensity of the received total emitted light is greatly increased, and compared with a traditional spectrophotometer, the sensitivity is higher.

The structure 3 may be a member for connecting and combining the parts in the portable device. In some embodiments, the structure 3 may comprise means for holding a solution to be tested. In some embodiments, the structure may be configured as a cuvette 301, with the cuvette 301 being coated with a reflective coating 302. Wherein cuvette 302 may be used to hold a solution to be tested; the reflective coating 302 may be used to reflect the fluorescence emitted by the solution to be tested, so that the detection result of the photodetector 2 is more accurate.

The electronic component 4 is a basic component in the circuit, and may include a power source 401, a wireless transmitter 402, and the like, for example. The power source 401 may be a device for supplying power to the apparatus, and the kind thereof is not limited, and may be a button cell, a number 7 cell, or the like. The wireless transmitter 402 may transmit the detection result of the photodetector 2 to a device terminal for displaying the detection result. In some embodiments, the device terminal may be a cell phone, a computer, or the like.

In some embodiments, the LED light source 1 is mounted on one side of the cuvette 301, the illumination detector 2 is mounted on any of the remaining sides of the cuvette 301 at a higher mounting position than the LED light source 1, and the LED light source 1 is electrically connected to the electronic component 4.

In detection, the solution to be detected is dripped into the cuvette 301, the LED light source 1 irradiates the solution to be detected, and the solution to be detected absorbs illumination to excite fluorescence. The photoelectric detector 2 senses fluorescence excited by the solution to be detected and fluorescence reflected by the reflective coating 302, converts an optical signal into an electrical signal, transmits the electrical signal to the wireless transmitter 402, transmits fluorescence intensity to a device terminal through the wireless transmitter 402, and calculates lithium ion concentration in the sample solution according to a linear relation between the fluorescence intensity excited by the solution to be detected and the lithium ion concentration in the sample solution.

In some embodiments, the feasibility of the portable device to detect lithium ion concentration may be verified by comparing the difference in detection lithium ion concentration between the portable device and the spectrophotometer. For further description of the spectrophotometer and the present portable device, see fig. 6 and its associated description.

In some embodiments of the present disclosure, the LED light source with a certain wavelength is used to emit light, so that the intensity and stability of the light source can be ensured, and meanwhile, the volume of the light source and the volume of the portable device are reduced, and the portability of the device is improved.

While the basic concepts have been described above, it will be apparent to those skilled in the art that the foregoing detailed disclosure is by way of example only and is not intended to be limiting. Although not explicitly described herein, various modifications, improvements, and adaptations to the present disclosure may occur to one skilled in the art. Such modifications, improvements, and modifications are intended to be suggested within this specification, and therefore, such modifications, improvements, and modifications are intended to be included within the spirit and scope of the exemplary embodiments of the present invention.

Meanwhile, the specification uses specific words to describe the embodiments of the specification. Reference to "one embodiment," "an embodiment," and/or "some embodiments" means that a particular feature, structure, or characteristic is associated with at least one embodiment of the present description. Thus, it should be emphasized and should be appreciated that two or more references to "an embodiment" or "one embodiment" or "an alternative embodiment" in various positions in this specification are not necessarily referring to the same embodiment. Furthermore, certain features, structures, or characteristics of one or more embodiments of the present description may be combined as suitable.

Furthermore, the order in which the elements and sequences are processed, the use of numerical letters, or other designations in the description are not intended to limit the order in which the processes and methods of the description are performed unless explicitly recited in the claims. While certain presently useful inventive embodiments have been discussed in the foregoing disclosure, by way of various examples, it is to be understood that such details are merely illustrative and that the appended claims are not limited to the disclosed embodiments, but, on the contrary, are intended to cover all modifications and equivalent arrangements included within the spirit and scope of the embodiments of the present disclosure. For example, while the system components described above may be implemented by hardware devices, they may also be implemented solely by software solutions, such as installing the described system on an existing server or mobile device.

Likewise, it should be noted that in order to simplify the presentation disclosed in this specification and thereby aid in understanding one or more inventive embodiments, various features are sometimes grouped together in a single embodiment, figure, or description thereof. This method of disclosure, however, is not intended to imply that more features than are presented in the claims are required for the present description. Indeed, less than all of the features of a single embodiment disclosed above.

In some embodiments, numbers describing the components, number of attributes are used, it being understood that such numbers being used in the description of embodiments are modified in some examples by the modifier "about," approximately, "or" substantially. Unless otherwise indicated, "about," "approximately," or "substantially" indicate that the number allows for a 20% variation. Accordingly, in some embodiments, numerical parameters set forth in the specification and claims are approximations that may vary depending upon the desired properties sought to be obtained by the individual embodiments. In some embodiments, the numerical parameters should take into account the specified significant digits and employ a method for preserving the general number of digits. Although the numerical ranges and parameters set forth herein are approximations that may be employed in some embodiments to confirm the breadth of the range, in particular embodiments, the setting of such numerical values is as precise as possible.

Each patent, patent application publication, and other material, such as articles, books, specifications, publications, documents, etc., referred to in this specification is incorporated herein by reference in its entirety. Except for application history documents that are inconsistent or conflicting with the content of this specification, documents that are currently or later attached to this specification in which the broadest scope of the claims to this specification is limited are also. It is noted that, if the description, definition, and/or use of a term in an attached material in this specification does not conform to or conflict with what is described in this specification, the description, definition, and/or use of the term in this specification controls.

Finally, it should be understood that the embodiments described in this specification are merely illustrative of the principles of the embodiments of this specification. Other variations are possible within the scope of this description. Thus, by way of example, and not limitation, alternative configurations of embodiments of the present specification may be considered as consistent with the teachings of the present specification. Accordingly, the embodiments of the present specification are not limited to only the embodiments explicitly described and depicted in the present specification.

Claims

1. A lithium ion measurement method, comprising: adding the sample solution into 1, 4-dihydroxyanthraquinone, an organic solvent and a strong alkali solution, and mixing to obtain a solution to be detected, wherein the concentration of the 1, 4-dihydroxyanthraquinone in the solution to be detected is 10-100 mu mol/L, and the structure of the 1, 4-dihydroxyanthraquinone

Is of the typeThe strong alkali comprises NaOH, and the concentration of the NaOH in the solution to be detected is 1.2-5 mmol/L; irradiating the solution to be detected with detection light to obtain the fluorescence intensity of the solution to be detected after irradiation; the wavelength of the detection light is 560-600nm; determining the lithium ion concentration in the sample solution based on the fluorescence intensity.

2. The method according to claim 1, wherein the concentration of 1, 4-dihydroxyanthraquinone in the solution to be tested is 10 to 40. Mu. Mol/L.

3. The method according to claim 1, wherein the concentration of NaOH in the test solution is 1.2-3.5 mmol/L.

4. The method of claim 1, wherein the determining the concentration of lithium ions in the sample solution based on the fluorescence intensity comprises:

and determining the lithium ion concentration in the sample solution based on a preset linear relation between the fluorescence intensity and the lithium ion concentration.

5. The method of claim 1, wherein the determining the concentration of lithium ions in the sample solution based on the fluorescence intensity comprises:

and determining the concentration of lithium ions in the sample solution based on the treatment of the fluorescence intensity by a determination model, wherein the determination model is a machine learning model.

6. A portable device for lithium ion measurement, the portable device being used for realizing the lithium ion measurement method according to claim 1, wherein the portable device comprises an LED light source, a photodetector, a structural member and an electronic component, wherein the wavelength of the LED light source is 560-600nm, the photodetector is used for obtaining the fluorescence intensity of the light source after irradiating a solution to be measured, the fluorescence intensity is used for determining the lithium ion solubility in a sample solution, the structural member at least comprises a member for containing the solution to be measured, and the electronic component at least comprises a power supply and a wireless emitter.

7. The apparatus as recited in claim 6, further comprising: the intensity integral of light with a wavelength range of 560-600nm has a ratio of more than a preset value in the total intensity integral in the LED light source.