CN103641543A

CN103641543A - Culture medium for prevention of astragalus mongolicus test-tube sprout etiolation

Info

Publication number: CN103641543A
Application number: CN201310593212.0A
Authority: CN
Inventors: 于亚军; 赵东利
Original assignee: Dalian University
Current assignee: Dalian University
Priority date: 2013-11-23
Filing date: 2013-11-23
Publication date: 2014-03-19

Abstract

A culture medium for prevention of astragalus mongolicus test-tube sprout etiolation belongs to the technical field of plant biology. The culture medium comprises a improved MS (Murashige and Skoog) culture medium and 30 g/L sucrose, the pH value is 5.8; the boric acid content of the improved MS culture medium is increased by 1 / 3 of the original content, the ammonium nitrate content of the improved MS culture medium is reduced by 1 / 5 of the original content, and a plant growth regulator is added. By selecting of the improved MS medium, the culture medium is simple and practicable, and has obvious long-lasting effects. Only by changing the trace substance boric acid content and the large amount substance ammonium nitrate content in the improved MS culture medium, the MS minimal medium can be optimized, the purpose of overcoming etiolation can be reached, and a test tube sprout can always keep green and healthy in proliferation subculturing and rooting processes.

Description

A kind of substratum that prevents the yellow of Radix Astagali test-tube plantlet

Technical field

The present invention relates to a kind of substratum that prevents the yellow of Radix Astagali test-tube plantlet, it belongs to plant biology technical field.

Background technology

Yellow is one of Three Difficult Issues of plant tissue culture.In the group training process of Radix Astagali, etiolation is serious.Adopt traditional MS substratum as minimum medium, add the plant-growth regulator of different sorts and concentration, still cannot overcome etiolation, finally cannot seedling.The traditional method that overcomes tissue cultured seedling yellow has: improve aeration status in bottle, increase sugared consumption, increase illumination, regulate hormone ratio etc., and the sealed membrane generally using in production or bottle cap often will be taken into account isolation internal and external environment, the interior aeration status of suitably ventilative and low three conditions of cost, so its bottle is generally changeless; With the increase of sugar amount, can increase the dangerous of pollution and improve production cost; In same experimental situation, be difficult to come capable of regulating illumination intensity and light application time for the happiness photosensitiveness of each kind of plant; Therefore, only have by optimizing minimum medium MS, more suitably add conventional Hormone, could thoroughly solve the yellow problem of Radix Astagali.

Summary of the invention

In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of substratum that prevents the yellow of Radix Astagali test-tube plantlet, and improve the culture effect of growth coefficient and rooting rate.

The present invention is achieved through the following technical solutions:

Prevent a substratum for Radix Astagali test-tube plantlet yellow, comprise modified MS medium+30g/L sucrose, pH value is 5.8; Modified MS medium comprises large quantity of material, micro substance, molysite and organism;

Component and its corresponding concentration of large quantity of material are as follows:

Ammonium nitrate 1320 mg/L;

Saltpetre 1900 mg/L;

Potassium primary phosphate 170 mg/L;

Magnesium sulfate heptahydrate 370 mg/L;

Calcium dichloride dihydrate 440mg/L;

The component of micro substance and its corresponding concentration are as follows:

Four water manganous sulfate 22.3mg/L;

Zinc Sulphate Heptahydrate 8.6 mg/L;

Boric acid 8.3 mg/L;

Potassiumiodide 0.83 mg/L;

Sodium Molybdate Dihydrate 0.25 mg/L;

Cupric sulfate pentahydrate 0.025 mg/L;

CoCL2 6H2O 0.025 mg/L;

The component of molysite and its corresponding concentration are as follows:

Iron vitriol 27.8 mg/L;

Disodium ethylene diamine tetraacetate 37.3 mg/L;

Organic component and its corresponding concentration are as follows:

Glycine 2.0 mg/L;

Vitamin 0.1 mg/L;

Pyridoxine hydrochloride 0.5 mg/L;

Nicotinic acid 0.5 mg/L;

Inositol 100 mg/L.

Substratum also comprises plant-growth regulator, and its kind and its corresponding concentration are 6-BA0.2g/L+IBA0.05g/L.

Substratum also comprises plant-growth regulator, and its kind and its corresponding concentration are IBA2.0g/L+NAA2.0 g/L.

Boron in boric acid is the essential factor of plant cell division and Protein formation, is conducive to the formation of cell walls, affects the nitrogen metabolism in plant materials, finally has influence on growing of plant.Boron deficiency or nitrogen content be unreasonable all may cause the even yellow of test-tube plantlet spike top.Reasonably the content of boric acid and the content of ammonium nitrate are the effective measure that overcome yellow.

The invention has the beneficial effects as follows: the present invention is by selecting modified MS medium, simple successful and lasting.Only need, by changing the content of ammonium nitrate in the content of micro substance boric acid in MS substratum and large quantity of material, to optimize MS minimum medium, can reach the object that overcomes yellow, in test-tube plantlet proliferation and subculture and rooting process, remain green healthy and strong.

Accompanying drawing explanation

Fig. 1 adopts to prevent the substratum of Radix Astagali test-tube plantlet yellow and traditional MS nutrition base comparison diagram;

Fig. 2 is the cultivation effect figure that adopts the substratum that prevents the yellow of Radix Astagali test-tube plantlet;

Fig. 3 is the rooting efficiency figure that adopts the substratum that prevents the yellow of Radix Astagali test-tube plantlet.

Embodiment

Below in conjunction with specific embodiment, describe the specific embodiment of the present invention in detail:

Embodiment 1

Modified MS medium+30g/L sucrose; PH value is adjusted to 5.8, and the component of the large quantity of material of modified MS medium and its corresponding concentration are as follows:

Ammonium nitrate 1320 mg/L;

Saltpetre 1900 mg/L;

Potassium primary phosphate 170 mg/L;

Magnesium sulfate heptahydrate 370 mg/L;

Calcium dichloride dihydrate 440 mg/L;

Four water manganous sulfate 22.3 mg/L;

Zinc Sulphate Heptahydrate 8.6 mg/L;

Boric acid 8.3 mg/L;

Potassiumiodide 0.83 mg/L;

Sodium Molybdate Dihydrate 0.25 mg/L;

Cupric sulfate pentahydrate 0.025 mg/L;

CoCL2 6H2O 0.15 mg/L;

The component of molysite and its corresponding concentration are as follows:

Iron vitriol 27.8 mg/L;

Disodium ethylene diamine tetraacetate 37.3 mg/L;

Organic component and its corresponding concentration are as follows:

Glycine 2.0 mg/L;

Vitamin 0.1 mg/L;

Pyridoxine hydrochloride 0.5 mg/L;

Nicotinic acid 0.5 mg/L;

Inositol 100 mg/L.

Technical process:

1) get Radix Astagali test-tube plantlet;

2) wipe out the new stubble of base portion;

3) be seeded in above-mentioned substratum;

4) intensity of illumination 2000lux, photoperiod 12h/d;

Result is: after 40 days, can form green seedling clump, and effect stability, and can not return to again yellow state.

Embodiment 2

Modified MS medium+30g/L sucrose, then add plant-growth regulator, its kind and its corresponding concentration are 6-BA0.2g/L+IBA0.05g/L.

Except substratum difference, all the other adopt the technical process identical with embodiment 1.

Result is: after 40 days, can form green seedling clump, its growth coefficient is up to 5.5, and test-tube plantlet growth is healthy and strong.

Embodiment 3

Modified MS medium+30g/L sucrose, then add plant-growth regulator, its kind and its corresponding concentration are IBA2.0g/L+NAA2.0 g/L.

Result is: rooting rate is higher, and seedling rate is high, and on the root media of screening, rooting rate reaches 85%.

The present invention optimizes MS minimum medium by the content of ammonium nitrate in the content by micro substance boric acid in change MS substratum and large quantity of material, can reach the object that overcomes yellow, in MS substratum except the content and ammonium nitrate content of boric acid, other component content is constant, and dosage of sucrose adopts conventional 30g/L.

1) content of MS substratum mesoboric acid increases 1/3 of original content.

2) in MS substratum, ammonium nitrate content reduces 1/5 of original content.

3) in test-tube plantlet propagation formula, kind and the content of plant-growth regulator is 6-BA0.2g/L+IBA0.05g/L.

4) in rooting of vitro seedling formula, the kind of plant-growth regulator and content are IBA2.0g/L+NAA2.0 g/L.

The present invention is applied on the Radix Astagali of yellow, after 40 days, can form green seedling clump, and its growth coefficient is up to 5.5, test-tube plantlet growth is healthy and strong, remains green state, can not return to yellow state again, on the root media of screening, rooting rate reaches 85%, finally seedling.With the content of boric acid and the optimization MS minimum medium of ammonium nitrate content change, the method that may alleviate etiolation with respect to other, has advantages of workable, simple.

Below with preferred embodiment, disclose the present invention, so it is not intended to limiting the invention, and all employings are equal to replaces or technical scheme that equivalent transformation mode obtains, within all dropping on protection scope of the present invention.

Claims

1. a substratum that prevents the yellow of Radix Astagali test-tube plantlet, is characterized in that: comprise modified MS medium+30g/L sucrose, pH value is 5.8; Modified MS medium comprises large quantity of material, micro substance, molysite and organism;

Ammonium nitrate 1320 mg/L;

Saltpetre 1900 mg/L;

Anhydrous potassium dihydrogenphosphate 170 mg/L;

Magnesium sulfate heptahydrate 370 mg/L;

Calcium dichloride dihydrate 440mg/L;

Four water manganous sulfate 22.3mg/L;

Zinc Sulphate Heptahydrate 8.6 mg/L;

Boric acid 8.3 mg/L;

Potassiumiodide 0.83 mg/L;

Sodium Molybdate Dihydrate 0.25 mg/L;

Cupric sulfate pentahydrate 0.025 mg/L;

CoCL2 6H2O 0.025 mg/L;

The component of molysite and its corresponding concentration are as follows:

Iron vitriol 27.8 mg/L;

Disodium ethylene diamine tetraacetate 37.3 mg/L;

Organic component and its corresponding concentration are as follows:

Glycine 2.0 mg/L;

Vitamin 0.1 mg/L;

Pyridoxine hydrochloride 0.5 mg/L;

Nicotinic acid 0.5 mg/L;

Inositol 100 mg/L.

2. a kind of substratum that prevents the yellow of Radix Astagali test-tube plantlet according to claim 1, is characterized in that: also comprise plant-growth regulator, its kind and its corresponding concentration are 6-BA0.2g/L+IBA0.05g/L.

3. a kind of substratum that prevents the yellow of Radix Astagali test-tube plantlet according to claim 1, is characterized in that: also comprise plant-growth regulator, its kind and its corresponding concentration are IBA2.0g/L+NAA2.0 g/L.