CN103636780B - A kind of acidified milk and preparation method thereof - Google Patents
A kind of acidified milk and preparation method thereof Download PDFInfo
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- CN103636780B CN103636780B CN201310646222.6A CN201310646222A CN103636780B CN 103636780 B CN103636780 B CN 103636780B CN 201310646222 A CN201310646222 A CN 201310646222A CN 103636780 B CN103636780 B CN 103636780B
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Abstract
The invention provides a kind of acidified milk and preparation method thereof.By 100 parts by weight, this acidified milk contains the Leuconostoc mesenteroides acidified milk of normal fermentation breast and at least 2 parts; Is described Leuconostoc mesenteroides acidified milk obtained by following method: by mixed uniformly raw milk and white granulated sugar heating, homogeneous, sterilization, inoculation Leuconostoc mesenteroides (Leuconostoc? mesenteroides) CGMCC? NO.6432 ferments, and obtains Leuconostoc mesenteroides acidified milk.Acidified milk raw material of the present invention is simple, and production technology simplifies, and do not add any additive and stabilizing agent, mouthfeel is smooth strong, has natural scents, is suitable for, good water-retaining property containing abundant probio viscosity, and in the shelf-life, no whey is separated out.
Description
Technical field
The present invention relates to a kind of acidified milk and preparation method thereof.
Background technology
Acidified milk of the prior art generally uses lactobacillus bulgaricus (Lactobacillus bulgaricus) and streptococcus thermophilus (Streptococcus thermophilus) bacterial classification to ferment, and can contain multiple additives and stabilizing agent.And acidified milk of the prior art, if not doping is as stabilizing agent and/or thickener etc., will poor stability, quality is loose, bleed is serious.
Acidified milk is popular health-oriented products, along with growth in the living standard and the attention to health, the healthy food not adding any additive is more and more subject to consumers, and developing a kind ofly does not become study hotspot containing the Novel fermented milk product of additive containing profitable probliotics.
Summary of the invention
The present invention is not ideal enough in order to solve acidified milk existence and stability of the prior art, and containing more additive, if it is loose to there is quality in not doping, the defect that bleed seriously waits, there is provided a kind of succinctly to fill a prescription, the mouthfeel that straightforward procedure is produced is strong, has natural scents and does not contain any additive, a kind of acidified milk of good stability, quality exquisiteness and preparation method thereof.
The invention provides a kind of acidified milk, by 100 parts by weight, it contains the Leuconostoc mesenteroides acidified milk of normal fermentation breast and at least 2 parts;
Described Leuconostoc mesenteroides acidified milk is obtained by following method:
Mixed uniformly raw milk and white granulated sugar heating, homogeneous, sterilization, inoculation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 are fermented, obtains Leuconostoc mesenteroides acidified milk.
In Leuconostoc mesenteroides acidified milk, described Leuconostoc mesenteroides (Leuconostocmesenteroides) the CGMCC NO.6432 inoculum concentration be inoculated in raw material milk is that this area is conventional; Preferably, described fermentation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 clump count in raw milk when starting is 1 × 10
6~ 1 × 10
8cfu/mL.
In Leuconostoc mesenteroides acidified milk, the addition of described white granulated sugar is that this area is conventional.Preferably account for 5 ~ 18% of raw milk and white granulated sugar gross mass.
In the present invention, preferably, described acidified milk is by 100 parts by weight, and it contains 80 ~ 98 parts of normal fermentation breasts and 2 ~ 20 parts of Leuconostoc mesenteroides acidified milks.
In the present invention, described raw milk is this area convenient source breast.Preferably, described raw milk is fresh milk or reconstituted milk.Better, time described raw milk is reconstituted milk, described white granulated sugar adds preparing in the middle of reconstituted milk process.
Wherein, described reconstituted milk is the conventional reconstituted milk in this area.Preferably, described reconstituted milk is obtained by following method: raw material contains by mass percentage: whole-fat milk powder 10 ~ 12%, water 88 ~ 90%; Above-mentioned raw materials is heated to 30 ~ 60 DEG C, 300 ~ 1500 turns/min mixing speed mixing 20 ~ 30 minutes, leaves standstill 1 ~ 10 hour at 4 ~ 30 DEG C, obtains reconstituted milk.
In the present invention, the condition of described heating is that this area is conventional, and preferably, the temperature of described heating is 40 ~ 65 DEG C, better, and the temperature of described heating is 40 ~ 60 DEG C.
In the present invention, the condition of described homogeneous is that this area is conventional, and preferably, the pressure of described homogeneous is 10MPa ~ 20MPa.
In the present invention, the condition of described sterilization is that this area is conventional, and preferably, the temperature of described sterilization is 90 ~ 95 DEG C, and the time of described sterilization is 5-10 minute.
In the present invention, the condition of described fermentation is that this area is conventional, and preferably, the temperature of described fermentation is 25-33 DEG C, and the time of fermentation is 15-48 hour.
In the present invention, preferably, described Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 accesses in fermentation seed liquid mode; Described fermentation seed liquid is obtained by this area conventional method.Preferably, obtained by following method: get-80 DEG C of Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 preserved, the culture dish of the M17 solid medium containing 5wt% sucrose activates, and spread cultivation step by step in the sterile absorbent Ruzhong containing 5wt% sucrose, cultivation temperature is 28 DEG C, incubation time is 32 hours, obtains fermentation seed liquid.
In the present invention, described normal fermentation breast is that this area is conventional.Preferably, described normal fermentation breast is obtained by following method: by fresh milk or reconstituted milk heating, carry out homogeneous, sterilization, cooling; Access leavening ferments.
Wherein, preferably can also add white granulated sugar before described heating, use amount can as previously mentioned, and the addition of described white granulated sugar is preferably for accounting for the 5%-10% of fresh milk or reconstituted milk quality.
Wherein, described reconstituted milk is the conventional reconstituted milk in this area, and preparation method as previously mentioned.
Wherein, described heating, homogeneous and sterilization operation are all as previously mentioned.
Wherein, describedly this area routine operation is cooled to.Preferably, the temperature of described cooling is 40 ~ 45 DEG C.
Wherein, described leavening is this area normal fermentation agent.Be generally the leavening containing lactobacillus bulgaricus (Lactobacillus bulgaricus) and streptococcus thermophilus (Streptococcus thermophilus), form is generally bacterium powder.Preferably, described leavening addition accounts for 0.01 ~ 1 ‰ of fresh milk or reconstituted milk quality; The temperature of described fermentation is 40 ~ 45 DEG C, and the time of described fermentation is 4 ~ 10 hours.
In the present invention, preferably, described acidified milk, by 100 parts by weight, is made up of 80 ~ 98 parts of normal fermentation breasts and 2 ~ 20 parts of Leuconostoc mesenteroides acidified milks.
The present invention also provides a kind of preparation method of above-mentioned acidified milk: described normal fermentation breast and described Leuconostoc mesenteroides acidified milk are mixed, obtain acidified milk of the present invention.
On the basis meeting this area general knowledge, above-mentioned each optimum condition, can be combined, obtain the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material are all commercially.
Positive progressive effect of the present invention is: acidified milk raw material of the present invention is simple, and production technology simplifies, and does not add any additive and stabilizing agent, mouthfeel is smooth strong, has natural scents, is suitable for containing abundant probio viscosity, good water-retaining property, in the shelf-life, no whey is separated out.
Detailed description of the invention
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, conventionally and condition, or selects according to catalogue.
Embodiment 1
1)-80 DEG C of Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCCNO.6432 preserved are got, the culture dish of the M17 solid medium containing 5% sucrose activates, and spread cultivation step by step in the sterile absorbent Ruzhong containing 5wt% sucrose, obtain fermentation seed liquid (cultivation temperature 28 DEG C), incubation time is 32 hours;
2) will comprise the heating raw materials mixing of following weight percents: fresh cow milk 90%, white granulated sugar 10%, heating-up temperature is 40 DEG C, while hot homogeneous (20MPa), sterilization processing, and sterilization temperature is 95 DEG C, and sterilizing time is 5 minutes;
3) fermentation seed liquid that step 1) obtains being seeded to above-mentioned steps 2) in the aseptic fresh cow milk that obtains, when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
6cfu/mL, 25 DEG C ferment 48 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: fresh milk (being heated to 50 DEG C)+white granulated sugar → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (42 DEG C, 4 hours) → cooling, stirring.Leavening is the leavening containing lactobacillus bulgaricus (Lactobacillus bulgaricus) and streptococcus thermophilus (Streptococcus thermophilus).Following embodiment is all identical.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 10:90 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 2
1) by embodiment 1 same method activation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432, fermentation seed liquid is obtained;
2) will comprise the heating raw materials mixing of following weight percents: fresh cow milk 82%, white granulated sugar 18%, heating-up temperature is 45 DEG C; Homogeneous (10MPa) while hot, sterilization processing, sterilization temperature is 90 DEG C, and sterilizing time is 10 minutes;
3) fermentation seed liquid that step 1) obtains being seeded to above-mentioned steps 2) in the aseptic fresh cow milk that obtains, when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
8cfu/mL, 33 DEG C ferment 15 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: fresh milk (being heated to 50 DEG C)+white granulated sugar → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (42 DEG C, 8 hours) → cooling, stirring.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 2:98 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 3
1) by embodiment 1 same method activation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432, fermentation seed liquid is obtained;
2) will comprise the heating raw materials mixing of following weight percents: fresh cow milk 95%, white granulated sugar 5%, heating-up temperature is 50 DEG C; Homogeneous (15MPa) while hot, sterilization processing, sterilization temperature is 88 DEG C, and sterilizing time is 8 minutes;
3) be seeded in above-mentioned aseptic fresh cow milk by the fermentation seed liquid that step 1) obtains, when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
8cfu/mL, 30 DEG C ferment 20 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: fresh milk (being heated to 50 DEG C)+white granulated sugar → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (42 DEG C, 10 hours) → cooling, stirring.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 6:94 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 4
1) by embodiment 1 same method activation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432, fermentation seed liquid is obtained;
2) heating raw materials comprising following weight percents is mixed with reconstituted milk: whole-fat milk powder 11.04%, white granulated sugar 8%, water 80.96%(water: whole-fat milk powder mass ratio=88:12), heating-up temperature is 45 DEG C, be uniformly mixed 20 ~ 30 minutes with 1000 turns/min mixing speed, 20 DEG C leave standstill 5 hours; Be heated to 60 ~ 65 DEG C, homogeneous, sterilization processing, sterilization temperature is 90 DEG C, and sterilizing time is 5 minutes;
3) be seeded in above-mentioned aseptic reconstituted milk by the fermentation seed liquid that step 1) obtains, when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
7cfu/mL, 30 DEG C ferment 25 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: fresh milk (being heated to 50 DEG C)+white granulated sugar → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (42 DEG C, 10 hours) → cooling, stirring.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 6:94 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 5
1) by embodiment 1 same method activation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432, fermentation seed liquid is obtained;
2) heating raw materials comprising following weight percents is mixed with reconstituted milk: whole-fat milk powder 9.2%, white granulated sugar 8%, water 82.8%(water: whole-fat milk powder mass ratio=90:10), heating-up temperature is 30 DEG C, be uniformly mixed 20 ~ 30 minutes with 1500 turns/min mixing speed, 30 DEG C leave standstill 1 hour; Be heated to 60 ~ 65 DEG C, homogeneous, sterilization processing, sterilization temperature is 90 DEG C, and sterilizing time is 5 minutes;
3) be seeded to by the fermentation seed liquid that step 1) obtains in above-mentioned aseptic reconstituted milk (w/w), when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
7cfu/mL, 30 DEG C ferment 25 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: fresh milk (being heated to 50 DEG C) → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (40 DEG C, 10 hours) → cooling, stirring.Leavening addition is 0.01 ‰.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 20:80 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 6
1) by embodiment 1 same method activation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432, fermentation seed liquid is obtained;
2) heating raw materials comprising following weight percents is mixed with reconstituted milk: whole-fat milk powder 10%, white granulated sugar 8%, water 82%, heating-up temperature is 60 DEG C, is uniformly mixed 20 ~ 30 minutes with 300 turns/min mixing speed, and 4 DEG C leave standstill 10 hours; Be heated to 60 ~ 65 DEG C, homogeneous, sterilization processing, sterilization temperature is 90 DEG C, and sterilizing time is 5 minutes;
3) be seeded to by the fermentation seed liquid that step 1) obtains in above-mentioned aseptic reconstituted milk (w/w), when fermentation is started, Leuconostoc mesenteroides clump count reaches 1 × 10
7cfu/mL, 30 DEG C ferment 25 hours, obtain Leuconostoc mesenteroides acidified milk.
4) normal fermentation breast is produced with common process: by the reconstituted milk containing white granulated sugar, be heated to 50 DEG C → high-speed stirred 20 minutes → be heated to 60 DEG C ~ 65 DEG C → homogeneous [first class pressure (16.5 ± 0.5) MPa, secondary pressure (3.5 ± 0.5) MPa] → sterilization (90 DEG C ~ 95 DEG C, 5 minutes) → be cooled to 40 DEG C ~ 44 DEG C → access leavening → fermentation (45 DEG C, 10 hours) → cooling, stirring.Leavening addition is 1 ‰.
By above-mentioned steps 3) and 4) acidified milk that obtains with the mixing of 20:80 weight ratio, stir, obtain acidified milk of the present invention.
Embodiment 7
All the other are with embodiment 1, by above-mentioned steps 3) and 4) acidified milk that obtains, and strawberry fruit juice is with the mixing of 9:90:1 weight ratio, stirs, obtains the acidified milk of the present embodiment.
Effect example 1
With a strain Leuconostoc mesenteroides LM79(available from DuPont company) be contrast, make Leuconostoc mesenteroides acidified milk in the same way, obtained acidified milk mixes (control group 1 with normal fermentation breast, the ratio that LM79 acidified milk accounts for gross weight is 6%), compare the characteristic containing two kinds of goldbeater's skin bacterium fermented dairy products.Embodiment 1 common process is used to produce normal fermentation breast as a control group 2.The acidified milk (embodiment 1 ~ 6 obtains sample) obtain the present invention and control group 1,2 carry out the mensuration of syneresis sensitiveness (bleed).
The bleed of fermented dairy product adopts the assay method of syneresis sensitiveness to measure.Under 25 DEG C of conditions, the funnel being placed with filter paper is positioned on test tube, to sampling sample gentle agitation, sample is evenly sought unity of standard, take the sample of 50g standard sample in funnel, place 30 minutes, collect the whey that drop goes out, and the whey of collecting is weighed, its computing formula is as follows: yogurt is to sensitiveness=whey amount of precipitation/example weight × 100% of syneresis.Every class sample replication 3 times, gets its mean value.
Effect example 2
The acidified milk (embodiment 1 ~ 6 obtains sample) obtain the present invention and control group 1,2 carry out sensory testing, and control group 1 and 2 is with effect example 1, and subjective appreciation is completed by 20 valuation officers.Person is effective for the first six index score height, and evaluate high, each full marks is 100, adds up 20 people and to give a mark sum.Result is as shown in the table:
Shown by above data, the present invention greatly improves the local flavor of product, adds mouthfeel, obtains better preference degree.Product can be made to have higher surcharge.Quality is finer and smoother cunning simultaneously, and local flavor delicate fragrance, mouthfeel is strong, has larger improvement.
Effect example 3
The present invention is carried out to the validity check of physical characteristic aspect containing the acidified milk of Leuconostoc mesenteroides, not add the normal fermentation breast of any stabilizing agent for contrast, use proRheo-180 viscosity meter, measurement parameter: No. 2 rotors, measuring tempeature 10 DEG C, rotating speed 64r/min, interval 10s.Acidified milk viscosity of the present invention is good.
Experimental result following (unit Pa.S):
Data display in above effect example 1-3, Leuconostoc mesenteroides (CGMCC NO.6432) acidified milk each index in mobility (viscosity) and water-retaining property has improvement clearly.Six groups of embodiment viscosity all higher than control sample and syneresis rate lower than control sample, prove that the acidified milk quality that the present invention obtains is better, be more suitable for edible and sell.Simultaneously subjective appreciation also obtain higher preference degree (overall assessment is that excellent ratio is much larger than control group).Therefore acidified milk of the present invention is compared with Traditional Fermented Milk, and local flavor, mouthfeel and quality all have obvious improvement.
Effect example 4
Its finished product physical and chemical index and the 1st day and (21 days) viable count of lactobacillus of guaranteeing the quality the end of term are measured to the acidified milk (embodiment 1 ~ 6 obtains sample) containing Leuconostoc mesenteroides of the present invention.Physical and chemical index presses GB19302-2010 standard test, and lactic acid bacteria number measures according to GB4789.35 method, and each sample surveys three times, gets its mean value.Result of the test is as follows:
1st day
| Project | Fat (g/100g) | Protein (g/100g) | Acidity OT |
| Embodiment 1 | 3.12 | 2.92 | 72 |
| Embodiment 2 | 3.09 | 2.86 | 71 |
| Embodiment 3 | 3.16 | 2.87 | 73 |
| Embodiment 4 | 3.18 | 2.94 | 72 |
| Embodiment 5 | 3.15 | 2.95 | 72 |
| Embodiment 6 | 3.18 | 2.93 | 73 |
21st day
| Project | Fat (g/100g) | Protein (g/100g) | Acidity OT |
| Embodiment 1 | 3.11 | 2.91 | 84 |
| Embodiment 2 | 3.09 | 2.88 | 85 |
| Embodiment 3 | 3.17 | 2.86 | 87 |
| Embodiment 4 | 3.16 | 2.93 | 86 |
| Embodiment 5 | 3.17 | 2.93 | 87 |
| Embodiment 6 | 3.16 | 2.96 | 87 |
Lactic acid bacteria number (CFU/g)
Above physics and chemistry and lactic acid bacteria number index result illustrate that this product meets the requirement of GB19302-2010 to flavored fermented milk, and product category can be denoted as " flavored fermented milk ".
Effect example 5
Its polyoses content is measured to acidified milk of the present invention (embodiment 1 ~ 6 obtains sample).Take 200g sample, add triploid and amass cold ethanol, slowly stir, leave standstill refrigerated overnight (4 DEG C, 16 hours), centrifugal collecting precipitation (4 DEG C, 9000rpm/min), adding trichloroacetic acid to accounting for 3% of final volume, leaving standstill refrigerated overnight (4 DEG C, 16 hours), collected by centrifugation supernatant (4 DEG C, 9000rpm/min), dialysis (molecule retains limit 14000Da) 72 hours, within every 8 hours, change water once, freeze-drying obtains polysaccharide.Dry to constant weight, take polysaccharide weight.Result is as follows:
Above experimental result shows, finished product is without the need to adding stabilizing agent and thickness, stable system is without lamination, mouthfeel and local flavor are all good, in 21 day shelf-life, product quality is stablized, and whey is separated out few, physical and chemical index, viable count all can reach the requirement of GB to flavored fermented milk, have certain market competitiveness.
Claims (13)
1. an acidified milk, is characterized in that, by 100 parts by weight, it contains the Leuconostoc mesenteroides acidified milk of normal fermentation breast and at least 2 parts; Described Leuconostoc mesenteroides acidified milk is obtained by following method:
Mixed uniformly raw milk and white granulated sugar heating, homogeneous, sterilization, inoculation Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 are fermented, obtains Leuconostoc mesenteroides acidified milk;
Described normal fermentation breast is obtained by following method: by fresh milk or reconstituted milk heating, carry out homogeneous, sterilization, cooling; Access leavening ferments.
2. acidified milk as claimed in claim 1, it is characterized in that, in the preparation process of described Leuconostoc mesenteroides acidified milk, described fermentation Leuconostoc mesenteroides (Leuconostocmesenteroides) CGMCC NO.6432 clump count in raw milk when starting is 1 × 10
6~ 1 × 10
8cfu/mL; And/or the addition of described white granulated sugar accounts for 5 ~ 18% of described raw milk and white granulated sugar gross mass.
3. acidified milk as claimed in claim 1, is characterized in that, by 100 parts by weight, it contains 80 ~ 98 parts of normal fermentation breasts and 2 ~ 20 parts of Leuconostoc mesenteroides acidified milks.
4. acidified milk as claimed in claim 3, is characterized in that, described acidified milk, by 100 parts by weight, is made up of 80 ~ 98 parts of normal fermentation breasts and 2 ~ 20 parts of Leuconostoc mesenteroides acidified milks.
5. acidified milk as claimed in claim 1, it is characterized in that, described raw milk is fresh milk or reconstituted milk.
6. acidified milk as claimed in claim 5, is characterized in that, time described raw milk is reconstituted milk, described white granulated sugar adds preparing in the middle of reconstituted milk process;
Wherein, described reconstituted milk is obtained by following method: raw material contains by mass percentage: whole-fat milk powder 10 ~ 12%, water 88 ~ 90%; Above-mentioned raw materials is heated to 30 ~ 60 DEG C, 300 ~ 1500 turns/min mixing speed mixing 20 ~ 30 minutes, leaves standstill 1 ~ 10 hour at 4 ~ 30 DEG C, obtains reconstituted milk.
7. acidified milk as claimed in claim 1, it is characterized in that, in the preparation process of described Leuconostoc mesenteroides acidified milk, the temperature of described heating is 40 ~ 65 DEG C;
And/or in the preparation process of described Leuconostoc mesenteroides acidified milk, the pressure of described homogeneous is 10MPa ~ 20MPa;
And/or in the preparation process of described Leuconostoc mesenteroides acidified milk, the temperature of described sterilization is 90 ~ 95 DEG C, and the time of described sterilization is 5-10 minute;
And/or in the preparation process of described Leuconostoc mesenteroides acidified milk, the temperature of described fermentation is 25-33 DEG C, and the time of described fermentation is 15-48 hour;
And/or described Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCCNO.6432 accesses in fermentation seed liquid mode.
8. acidified milk as claimed in claim 7, it is characterized in that, the temperature of described heating is 40 ~ 60 DEG C.
9. acidified milk as claimed in claim 7, it is characterized in that, described fermentation seed liquid is obtained by following method: get-80 DEG C of Leuconostoc mesenteroides (Leuconostoc mesenteroides) CGMCC NO.6432 preserved, the culture dish of the M17 solid medium containing 5wt% sucrose activates, and spread cultivation step by step in the sterile absorbent Ruzhong containing 5wt% sucrose, cultivation temperature is 28 DEG C, and incubation time is 32 hours, obtains fermentation seed liquid.
10. acidified milk as claimed in claim 1, it is characterized in that, described leavening is the leavening containing lactobacillus bulgaricus (Lactobacillus bulgaricus) and streptococcus thermophilus (Streptococcusthermophilus).
11. acidified milks as claimed in claim 1, is characterized in that, described also adds white granulated sugar by before fresh milk or reconstituted milk heating;
And/or the temperature of described cooling is 40 ~ 45 DEG C;
And/or in the preparation process of described normal fermentation breast, described leavening addition accounts for 0.01 ~ 1 ‰ of fresh milk or reconstituted milk quality; The temperature of described fermentation is 40 ~ 45 DEG C, and the time of described fermentation is 4 ~ 10 hours;
And/or in the preparation process of described normal fermentation breast, described reconstituted milk is the reconstituted milk as described in claim 5 or 6;
And/or in the preparation process of described normal fermentation breast, described heating, homogeneous, sterilization are as described in claim 7 or 8.
12. acidified milks as claimed in claim 11, it is characterized in that, the addition of described white granulated sugar is the 5%-10% accounting for fresh milk or reconstituted milk quality.
The preparation method of 13. 1 kinds of acidified milks as described in any one of claim 1 ~ 12: by as described in normal fermentation breast and as described in Leuconostoc mesenteroides acidified milk mix.
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| CN115336631A (en) * | 2022-08-22 | 2022-11-15 | 青岛景轩三辰生物科技有限公司 | Preparation method of active lactobacillus beverage and product |
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| CN101697752A (en) * | 2009-11-04 | 2010-04-28 | 内蒙古伊利实业集团股份有限公司 | Sour milk added with casein phosphopeptide and preparation method thereof |
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