CN103627386A - 一种荧光探针叶酸功能化的荧光金纳米簇的制备方法 - Google Patents
一种荧光探针叶酸功能化的荧光金纳米簇的制备方法 Download PDFInfo
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- CN103627386A CN103627386A CN201310638673.5A CN201310638673A CN103627386A CN 103627386 A CN103627386 A CN 103627386A CN 201310638673 A CN201310638673 A CN 201310638673A CN 103627386 A CN103627386 A CN 103627386A
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- folic acid
- gold nanoclusters
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- fluorescent gold
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims abstract description 52
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 229960000304 folic acid Drugs 0.000 title claims abstract description 26
- 235000019152 folic acid Nutrition 0.000 title claims abstract description 26
- 239000011724 folic acid Substances 0.000 title claims abstract description 26
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- 238000002360 preparation method Methods 0.000 title claims description 7
- DVGHHMFBFOTGLM-UHFFFAOYSA-L fluorogold Chemical compound F[Au][Au]F DVGHHMFBFOTGLM-UHFFFAOYSA-L 0.000 title description 21
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052737 gold Inorganic materials 0.000 claims abstract description 34
- 239000010931 gold Substances 0.000 claims abstract description 34
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- 239000000243 solution Substances 0.000 claims description 23
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- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 abstract description 7
- -1 mercury ions Chemical class 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
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- BQPIGGFYSBELGY-UHFFFAOYSA-N mercury(2+) Chemical compound [Hg+2] BQPIGGFYSBELGY-UHFFFAOYSA-N 0.000 description 9
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- JJWSNOOGIUMOEE-UHFFFAOYSA-N Monomethylmercury Chemical compound [Hg]C JJWSNOOGIUMOEE-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
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CN201310638673.5A CN103627386B (zh) | 2013-12-04 | 2013-12-04 | 一种荧光探针叶酸功能化的荧光金纳米簇的制备方法 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI511971B (zh) * | 2014-12-22 | 2015-12-11 | Ind Tech Res Inst | 探針、偵測金屬離子的方法、與偵測化學/生化分子的方法 |
CN105482809A (zh) * | 2015-11-30 | 2016-04-13 | 南京邮电大学 | 一种硫氢根离子纳米探针材料及其制备方法和应用 |
CN105572087A (zh) * | 2016-01-11 | 2016-05-11 | 东北师范大学 | 一种可循环利用的汞离子比色检测试纸及其应用方法 |
CN106583747A (zh) * | 2016-12-06 | 2017-04-26 | 南华大学 | 鱼精蛋白金纳米簇的制备及在模拟酶比色和荧光检测中的应用 |
CN106872429A (zh) * | 2017-03-08 | 2017-06-20 | 中南大学 | 一种荧光检测atp的工艺 |
CN112630199A (zh) * | 2020-11-10 | 2021-04-09 | 河北大学 | 一种基于合金纳米簇的叶酸含量检测方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009078924A2 (en) * | 2007-12-06 | 2009-06-25 | The Regents Of The University Of California | Mesoporous silica nanoparticles for biomedical applications |
CN102029401A (zh) * | 2010-11-30 | 2011-04-27 | 厦门大学 | 叶酸受体靶向型纳米金颗粒的合成方法 |
CN102703060A (zh) * | 2012-06-08 | 2012-10-03 | 中国药科大学 | 一种靶向特性的可示踪贵金属荧光探针及抗肿瘤前药 |
CN102989015A (zh) * | 2012-12-10 | 2013-03-27 | 西安交通大学 | 一种叶酸受体靶向肿瘤的ct成像分子探针的制备方法 |
CN103226103A (zh) * | 2013-04-04 | 2013-07-31 | 哈尔滨师范大学 | 一种汞离子比色检测探针及其应用方法 |
EP2634179A1 (en) * | 2012-02-28 | 2013-09-04 | Sanofi | Functional PLA-PEG copolymers, the nanoparticles thereof, their preparation and use for targeted drug delivery and imaging |
-
2013
- 2013-12-04 CN CN201310638673.5A patent/CN103627386B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009078924A2 (en) * | 2007-12-06 | 2009-06-25 | The Regents Of The University Of California | Mesoporous silica nanoparticles for biomedical applications |
CN102029401A (zh) * | 2010-11-30 | 2011-04-27 | 厦门大学 | 叶酸受体靶向型纳米金颗粒的合成方法 |
EP2634179A1 (en) * | 2012-02-28 | 2013-09-04 | Sanofi | Functional PLA-PEG copolymers, the nanoparticles thereof, their preparation and use for targeted drug delivery and imaging |
CN102703060A (zh) * | 2012-06-08 | 2012-10-03 | 中国药科大学 | 一种靶向特性的可示踪贵金属荧光探针及抗肿瘤前药 |
CN102989015A (zh) * | 2012-12-10 | 2013-03-27 | 西安交通大学 | 一种叶酸受体靶向肿瘤的ct成像分子探针的制备方法 |
CN103226103A (zh) * | 2013-04-04 | 2013-07-31 | 哈尔滨师范大学 | 一种汞离子比色检测探针及其应用方法 |
Non-Patent Citations (3)
Title |
---|
GAIPING LI等: "One-Step Synthesis of Folic Acid Protected Gold Nanoparticles and Their Receptor-Mediated Intracellular Uptake", 《CHEM. EUR. J.》, vol. 15, 31 December 2009 (2009-12-31) * |
N VASIMALAI等: "A turn-on highly selective and ultrasensitive determination of copper (II) in an aqueous medium using folic acid capped gold nanoparticles as the probe", 《NANOTECHNOLOGY》, vol. 24, 27 November 2013 (2013-11-27), XP020255240, DOI: 10.1088/0957-4484/24/50/505503 * |
柴芳: "基于金纳米颗粒生物探针的制备及其检测重金属离子的应用研究", 《东北师范大学 博士毕业论文》, 31 December 2010 (2010-12-31) * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI511971B (zh) * | 2014-12-22 | 2015-12-11 | Ind Tech Res Inst | 探針、偵測金屬離子的方法、與偵測化學/生化分子的方法 |
CN105717077A (zh) * | 2014-12-22 | 2016-06-29 | 财团法人工业技术研究院 | 探针、探测金属离子的方法、与探测化学/生化分子的方法 |
US9664664B2 (en) | 2014-12-22 | 2017-05-30 | Industrial Technology Research Institute | Probe and method for detecting metal ions and chemical/biochemical molecules |
CN105482809A (zh) * | 2015-11-30 | 2016-04-13 | 南京邮电大学 | 一种硫氢根离子纳米探针材料及其制备方法和应用 |
CN105482809B (zh) * | 2015-11-30 | 2017-12-05 | 南京邮电大学 | 一种硫氢根离子纳米探针材料及其制备方法和应用 |
CN105572087A (zh) * | 2016-01-11 | 2016-05-11 | 东北师范大学 | 一种可循环利用的汞离子比色检测试纸及其应用方法 |
CN106583747A (zh) * | 2016-12-06 | 2017-04-26 | 南华大学 | 鱼精蛋白金纳米簇的制备及在模拟酶比色和荧光检测中的应用 |
CN106872429A (zh) * | 2017-03-08 | 2017-06-20 | 中南大学 | 一种荧光检测atp的工艺 |
CN112630199A (zh) * | 2020-11-10 | 2021-04-09 | 河北大学 | 一种基于合金纳米簇的叶酸含量检测方法 |
CN112630199B (zh) * | 2020-11-10 | 2022-10-21 | 河北大学 | 一种基于合金纳米簇的叶酸含量检测方法 |
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Effective date of registration: 20180213 Address after: 330114 Jiangxi city of Nanchang province new village natural village Fang Zhen Xia District Lehua No. 7 of 3. Patentee after: Lu Jing Address before: 150025 School of chemistry and chemical engineering, No. 1, Harbin Normal University, Li Min Development Zone, Harbin, Heilongjiang Patentee before: Harbin Teachers' Univ. |
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Address after: 313011 No. 1, No. 5, B block, Xiaodi double Industrial Park, 288 Yan San Road, old town, Huzhou, Zhejiang Patentee after: Lu Jing Address before: 330114 No. 7, No. 3 natural village, Lao Wu Village, Yue Hua Town, new town, Nanchang, Jiangxi. Patentee before: Lu Jing |
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Effective date of registration: 20190923 Address after: 233700 Huahai Facade 2 # 105, No. 3 Road, Guzhen County, Bengbu City, Anhui Province Patentee after: Bengbu Geshi Intellectual Property Operations Co., Ltd. Address before: 313011 No. 5, 1st floor, Block B, Small and Double-wound Industrial Park, 288 Yansan Road, Jiuguan Town, Huzhou City, Zhejiang Province Patentee before: Lu Jing |