CN103627329B - A kind of method extracting gelatin from tailing - Google Patents
A kind of method extracting gelatin from tailing Download PDFInfo
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- CN103627329B CN103627329B CN201310596206.0A CN201310596206A CN103627329B CN 103627329 B CN103627329 B CN 103627329B CN 201310596206 A CN201310596206 A CN 201310596206A CN 103627329 B CN103627329 B CN 103627329B
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Abstract
The present invention relates to a kind of method extracting gelatin from tailing, the method comprises tailing acidleach, boils glue, flocculation, centrifugal, collects liquid, be gelatin solution.Tailing treatment process provided by the invention, the treatment process of more traditional tailing, glue protein content, gelatin viscosity, glue power of congealing all is greatly improved.
Description
Technical field
The present invention relates to the preparation method of gelatin, be specifically related to a kind of method extracting gelatin from tailing.
Background technology
Gelatin is the hydrolysate of collagen, is a kind of fat free high protein, and not containing cholesterol, is a kind of food thickener of native trophism.Both people can not have been made to get fat after edible, also can not cause physically-draining.Gelatin or a kind of strong protective colloid, emulsifying power is strong, can suppress the cohesion that the protein such as milk, soya-bean milk causes because of hydrochloric acid in gastric juice effect, thus be conducive to food digestion after entering stomach.
Gelatin is peptide molecule polymeric material, it is the hydrolysate of collagenic protein, so can be used as a kind of additive, the edible Gelatinum oxhide that we produce has the physicochemical property of many uniquenesses and higher nutritive value because of it, be rich in 18 seed amino acids that human body is necessary, it directly can make thick soup, jellied pork skin, carnivorous can, and crystal-jelley, salad, yolk juice, frosting, cream confection, fragrance sauce, chocolate, beverage, beer etc. eat for people.Technical gelatine is colourless to pale yellow transparent or thin slice or the powder such as translucent.Tasteless, odorless.Water-swelling in cold water.Its molecular weight is 1-7 ten thousand, and senior gelatine molecular weight is within 10-15 ten thousand.Gelatin is a kind of very high containing the very low and interior propylhomoserin of methyllanthionine, glycine, proline(Pro) and oxyproline equal size. gelatin is not soluble in cold water, but the weight that can absorb cold water is self 5-10 times, be soluble in warm water, cooling forms gel, glue fusing point is between 24-28 DEG C, its solubleness differs very little with temperature of solidification, be subject to moisture content, temperature, humidity impact and go bad.
The preparation method of bone gelatin(e) has: acid system, alkaline process, enzyme process and salt and alkali method etc.Wherein acid system adhesive-preparing technology only accounts for about 10%, but the acid system glue cycle is short, and productive rate is large.Its concrete preparation method is: with fresh ox-hide and pigskin for raw material, the fresh bone skin of strict screening, by repeatedly washing leaching, degreasing neutralization, cooking and liquefaction, sterilising filtration, concentrates the tens procedure streamlines such as oven dry and makes.The gelatin produced is a kind of tasteless, colourless (slightly light yellow), translucent, hard non-crystalline state thing, and it is insoluble to organic solvent, and its water-absorbent is strong, viscosity is high.
The extraction of gelatin generally needs carrying out higher than on the shrinkage temperature of collegen filament.Lesser temps extracts can obtain well-behaved gelatin, but productive rate is low, extraction time is long.It is gelatin that raising temperature can accelerate collagen turnover, if but temperature is too high, overlong time, gelatin will be made to be hydrolyzed further (secondary degraded), and what main peptide chain fracture occurred and greatly destroys gelatin freezes the important performance indexes such as power and viscosity, and the quality of gelatin is had a strong impact on.
Ding Fengping discloses a kind of acid system adhesive-preparing technology (Ding Fengping, the research of GA glue acid method adhesive-preparing technology, Chinese Journal of Pharmaceuticals, 1991,22(10): 433-436) in its summary part, write pigskin pickling used salt acid concentration 0.2mol/L exactly, the pickling time is 5-7h, absolute liquid coefficient 1.0-1.4, and boiling glue temperature is 55 DEG C, time 6-8h, can improve yield and the concentration of slubbing glue.
China's acid system glue works is mainly based on the skin gelatin of production edible gum.And acid system bone gelatin(e) is due to it, and to put forward glue quality unstable, and gelatin quality is not also greatly improved, and therefore seldom has batch production.
When adopting aggregate to prepare gelatin, remaining tailings (containing collagen protein no more than 6%) adopts the method process of high-temperature boiling usually, and the gelatin productive rate that this method is extracted is low, and glue is second-rate.
Contriver looks for another way, and have developed a kind of acidic process and to give up the adhesive-preparing technology of aggregate, improve gelatin yield and glue quality, for suitability for industrialized production creates more Multi benefit.
Summary of the invention
The object of this invention is to provide a kind of method extracting gelatin from tailing.
A kind of method extracting gelatin from tailing provided by the invention, the method comprises tailing acidleach, boils glue, flocculation, centrifugal, collects liquid, be gelatin solution.
In aforesaid method:
Described tailing is the moisture aggregate of ox bone after superheated water carries glue 3-4 time of gelatin factory, collagen content 3%-6% wherein.
Described acidleach is that 1:2-2:1 mixes by tailing and deionized water according to weight ratio, and then adding concentration is that the acid solution of 0.4-1.6% stirs; The weight of acid solution and the tailing volume ratio mg/mL after adding water are 0.4%-1.6%.
Described acid is sulfuric acid, sulfurous acid, hydrochloric acid, phosphoric acid;
The described glue that boils maintains feed temperature at 80-110 DEG C, and the reaction times is 10-40min;
Described flocculation is that reacted glue is added flocculation agent; Described flocculation agent is polyacrylamide flocculation agent;
Described centrifugal time rotating speed be: 4000-8000r/min, be preferably 6000r/min, the time is 15-20min.
Concrete, the method for described acid system glue, the method comprises the following steps:
1) preparation of feed liquid: be that 1:2-2:1 mixes according to weight ratio by tailing and ionized water, obtains feed liquid;
2) adding concentration to feed liquid is that the acid solution of 0.4-1.6% stirs, the weight of acid solution with add water after tailing volume ratio mg/mL be 0.4%-1.6%;
3) maintain feed temperature at 80-110 DEG C, the reaction times is 10-40min;
4) reacted glue is added polyacrylamide flocculation agent to feed clarification, then according to the centrifugation 15-20min of 4000-8000r/min, obtain gelatin solution.
Preferably, the method comprises the following steps:
1) preparation of feed liquid: be the tailing of 3%-6% and ionized water by collagen content be that 1-2:1 mixes according to weight ratio, obtains feed liquid;
2) adding concentration to feed liquid is that the acid solution of 0.4-1.6% stirs, acid solution weight with add water after the volume ratio of tailing be 0.8%-1.6%;
3) maintain feed temperature at 90-105 DEG C, the reaction times is 20-30min;
4) reacted glue is added polyacrylamide flocculation agent to feed clarification, then according to the centrifugation 15-20min of 5000-7000r/min, collect liquid, obtain gelatin solution.
Acid system glue provided by the invention has the following advantages:
1, traditional technology extracts with the direct high-temperature boiling of ox bone, and after extracting 3-4 time, remaining tailing uses high temperature extraction again, and extraction yield is low, and glue is of poor quality, and method provided by the invention has following advantage:
(1) adopt acid system adhesive-preparing technology, carrying glue only needs to complete in the short period of time, substantially reduces the production cycle, saves a large amount of energy, thus reduce production cost.
(2) adopt acid system adhesive-preparing technology, instead of traditional high temperature gelatin extraction technique, substantially reduce and carry the glue time, what improve glue freezes power and viscosity.
(3) adopt acid system adhesive-preparing technology, improve the yield of gelatin.
2, the raw material adopted in the present invention is the residue aggregate in traditional gelatin extraction technique after repeatedly carrying glue, belongs to refuse and again utilizes, and from this angle, reduces the cost of Pollutant Treatment, decreases environmental pollution, thus bring larger economic benefit.
3, reaction mechanism of the present invention is that acid is reacted with the collagen protein in ossein, through acidolysis, collagen protein partial hydrolysis is wherein become polypeptide, obtained gelatin.
4, tailing treatment process provided by the invention, the treatment process of more traditional tailing, glue protein content, gelatin viscosity, glue power of congealing all is greatly improved.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Traditional carries gluing method: get after ox bone 50-70 DEG C hot water carries glue 3-4 time; 2-3 hour extraction tail glue is boiled again with the hot water of 100 DEG C.
The present invention is in order to describe the problem, and it is the same that the tailing adopted extracts tailing used with traditional tailing, all adopts ox bone hot water to carry glue 3-4 rear remaining aggregate.
Collagen content in described tailing: 3-6%, moisture: 91.35-92.86%, ash content: 0.4-0.5%.
Embodiment 1: the method extracting gelatin from tailing
Take 6kg tailing (tailing collagen content: 4.107%, moisture: 92.86%, ash content: 0.46%), deionized water is added than for 1:1 according to bone water, mixing, add the sulfuric acid (sulfuric acid concentration is 98%) of 0.8% of material liquid volume again, heat in water-bath after stirring evenly, be heated to 90 DEG C in pot after, insulation 30min, then takes out feed liquid and adds polyacrylamide flocculation agent wherein to feed clarification, use settling centrifuge according to the centrifugation 15min of 6000r/min again, collect filtrate, obtain glue 10kg, wherein collagen content: 2.688%.
Embodiment 2: the method extracting gelatin from tailing
Take 6kg tailing (tailing collagen content: 3.441%, moisture: 92.37%, ash content: 0.5%), be that 2:1 adds deionized water according to material-water ratio, mixing, then add material liquid volume 1.6% sulfuric acid (sulfuric acid concentration is 98%), put into pot after stirring evenly and heat.Be heated to 100 DEG C in pot after, insulation 20min, then takes out feed liquid and adds polyacrylamide wadding flocculation agent wherein to feed clarification, then carry out solid-liquid separation with settling centrifuge.After process, glue is weighed, feed liquid heavy 7.8kg, wherein collagen content: 2.919%.
Embodiment 3: the method extracting gelatin from tailing
Take 6kg tailing (tailing collagen content: 4.662%, moisture: 91.35%, ash content: 0.4%) is that 1:1 adds deionized water mixing according to material-water ratio, add the sulfuric acid (sulfuric acid concentration is 98%) of 1.0% of material liquid volume again, bandage after stirring evenly and put into pressure kettle and heat.Be heated to 105 DEG C in pot after, insulation 20min, then takes out feed liquid and adds polyacrylamide flocculation agent wherein to feed clarification, then carry out solid-liquid separation with settling centrifuge.After process, glue is weighed, feed liquid heavy 11kg, wherein collagen content: 2.813%.
Comparative example 1: traditional tailing treatment process
Traditional tailing process: take 6Kg tailing (collagen content: 4.21%, moisture: 92.78%, ash content: 0.48%) add deionized water to covering all aggregates in tailing, then feed liquid is heated to 100 DEG C, boil 3 hours, gelatin solution 9.8kg is collected, wherein collagen content: 2.134% after process.This tail glue extract yield 83%, gained gelatin jelly power 105g.
Experimental example 1:
1, the gelatin yield extracted and gelatin finished product are detected, wherein:
Jelly power (singly freezing power) condition determination: 12% moisture, gelatine content 6.67% when, the Gel strength under 10 DEG C of environment;
Gumminess measures: the viscosity measuring gelatin with Engler viscosimeter, and it is that 10%(such as 10g gelatin is dissolved in 100ml water that gelatin water makes concentration).
2, the results are shown in Table 1:
Table 1:
Table 1 result shows: gelatin yield prepared by embodiment 1-3 is high, and jelly power is good, and gelatin yield prepared by comparative example 1 is low, and jelly power is poor.
Result shows: tailing provided by the invention carries gluing method, and the gelatin yield of extraction increases, and what improve glue freezes power and viscosity, is better than the treatment process of traditional tailing.
Although above with general explanation, embodiment and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (3)
1. from tailing, extract a method for gelatin, the method comprises the following steps:
Take 6kg tailing, tailing collagen content: 4.107%, moisture: 92.86%, ash content: 0.46%, adds deionized water according to bone water than for 1:1, mixing, add the sulfuric acid of 0.8% of material liquid volume again, sulfuric acid concentration is 98%, heats after stirring evenly in water-bath, be heated to 90 DEG C in pot after, insulation 30min, then take out feed liquid and add polyacrylamide flocculation agent wherein to feed clarification, then use settling centrifuge according to the centrifugation 15min of 6000r/min, collect filtrate.
2. from tailing, extract a method for gelatin, the method comprises the following steps:
Take 6kg tailing, tailing collagen content: 3.441%, moisture: 92.37%, ash content: 0.5%, be that 2:1 adds deionized water according to material-water ratio, mixing, then add material liquid volume 1.6% sulfuric acid, sulfuric acid concentration is 98%, put into pot after stirring evenly to heat, be heated to 100 DEG C in pot after, insulation 20min, then take out feed liquid and add polyacrylamide wadding flocculation agent wherein to feed clarification, then carry out solid-liquid separation with settling centrifuge.
3. from tailing, extract a method for gelatin, the method comprises the following steps:
Take 6kg tailing, tailing collagen content: 4.662%, moisture: 91.35%, ash content: 0.4%, according to material-water ratio be 1:1 add deionized water mixing, add the sulfuric acid of 1.0% of material liquid volume again, sulfuric acid concentration is 98%, bandages to put into pressure kettle and heat after stirring evenly, be heated to 105 DEG C in pot after, insulation 20min, then takes out feed liquid and adds polyacrylamide flocculation agent wherein to feed clarification, then carry out solid-liquid separation with settling centrifuge.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2008007173A (en) * | 2008-06-05 | 2009-12-07 | Univ Mexico Nacional Autonoma | Collagen sponges obtained from mammal bones, process for the obtention and uses thereof. |
| CN102433075A (en) * | 2011-11-13 | 2012-05-02 | 逯益民 | Method for quickly preparing special gelatin for candies from raw bone meal |
| CN102827550A (en) * | 2012-09-07 | 2012-12-19 | 罗赛洛(大安)明胶有限公司 | Gelatin preparation process for acid process bone element gelatin |
| CN103122219A (en) * | 2011-11-17 | 2013-05-29 | 中国科学院理化技术研究所 | Method for preparing gelatin by taking bone as raw material |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2008007173A (en) * | 2008-06-05 | 2009-12-07 | Univ Mexico Nacional Autonoma | Collagen sponges obtained from mammal bones, process for the obtention and uses thereof. |
| CN102433075A (en) * | 2011-11-13 | 2012-05-02 | 逯益民 | Method for quickly preparing special gelatin for candies from raw bone meal |
| CN103122219A (en) * | 2011-11-17 | 2013-05-29 | 中国科学院理化技术研究所 | Method for preparing gelatin by taking bone as raw material |
| CN102827550A (en) * | 2012-09-07 | 2012-12-19 | 罗赛洛(大安)明胶有限公司 | Gelatin preparation process for acid process bone element gelatin |
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Effective date of registration: 20170209 Address after: Victory road 233000 Anhui city of Bengbu province Yuhui District No. 777 Patentee after: Anhui BBCA Group Co., Ltd. Address before: With five on the south side of the road 233030 Anhui Huaishang district city of Bengbu Province Patentee before: Anhui Fengyuan Fermentation Technology Engineering Research Co., Ltd. |
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Effective date of registration: 20200619 Address after: 233700 Jinger Road East, Weisan Road South, LianZhan Economic Development Zone, Guzhen County, Bengbu City, Anhui Province Patentee after: Anhui Fengyuan gelatin Co., Ltd Address before: Victory road 233000 Anhui city of Bengbu province Yuhui District No. 777 Patentee before: ANHUI BBCA GROUP Co.,Ltd. |
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