CN103623026A - Combined medicament of pseudo-ginseng extract, ligusticum wallichii extract and red flower extract as well as preparation and application thereof - Google Patents
Combined medicament of pseudo-ginseng extract, ligusticum wallichii extract and red flower extract as well as preparation and application thereof Download PDFInfo
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- CN103623026A CN103623026A CN201310687449.5A CN201310687449A CN103623026A CN 103623026 A CN103623026 A CN 103623026A CN 201310687449 A CN201310687449 A CN 201310687449A CN 103623026 A CN103623026 A CN 103623026A
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Abstract
The invention discloses a combined medicament of pseudo-ginseng extract, ligusticum wallichii extract and red flower extract and a preparation thereof, relates to an extracting and combining method of a medicinal preparation and a preparation method thereof, and more specifically relates to an extracting and combining method of a pseudo-ginseng extract, a ligusticum wallichii extract and a red flower extract and a preparation method thereof. The combined medicine consists of the pseudo-ginseng extract, the ligusticum wallichii extract and the red flower extract at a weight ratio of (14-56):(7.5-30):(14-56), wherein total saponins contained in the pseudo-ginseng extract are not less than 20.0%, ferulic acid contained in the ligusticum wallichii extract is not less than 0.2%, and hydroxyl carthamin yellow A contained in the red flower extract is not less than 1.0%. After the pseudo-ginseng extract, the ligusticum wallichii extract and the red flower extract are prepared into the combined medicament, the amounts of the total saponins, the ferulic acid and the hydroxyl carthamin yellow A which are contained in per dose unit are respectively in the following weight ranges: 4.8-19.19mg, 0.017-0.067mg and 0.15-0.61mg.
Description
Technical field
What the composition of medicine of a kind of Radix Notoginseng extract of the present invention, Rhizoma Chuanxiong extract and Flos Carthami extract and preparation thereof, application related to is a kind of extraction, combined method and preparation method of pharmaceutical preparation, more particularly, the extraction, combined method and the preparation thereof that relate to a kind of Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract.
Background technology
Cardiovascular disease is that the whole world causes dead the first disease.For number, in global cardiovascular patient, especially cardiovascular disease high-risk patient necessarily, how by effective intervention means, reduce cardiovascular disease incidence rate and mortality rate, become a day by day urgent great public health problem.In China, cardiovascular and cerebrovascular disease is the first disease that causes human mortality.China dies from nearly 3,000,000 people of cardiovascular disease person every year, and crowd's quantity of suffering from all kinds of risk factors of cardiovascular diseases is also through hundred million.At present, aged tendency of population, day by day improving of living standard makes China just enter the concentrated phase of cardiovascular disease outburst, has the huge consumer group of cardiovascular drugs.At present the disease such as angina pectoris is very common clinically, and coronary heart disease refers to that coronary atherosclerosis makes the narrow or spasm of lumen of vessels cause myocardial ischemia-anoxemia and the heart disease that causes.Plain edition angina pectoris refers to that the classical angina being caused by myocardial ischemia-anoxemia shows effect.Although Chinese medicine diagnosis and treatment cardiovascular disease comes into one's own in recent years day by day, also have some limitations, the Chinese medicine of therefore finding the treatment cardiovascular and cerebrovascular disease of rapid-action, effective, stable curative effect be current in the urgent need to.
The Chinese medicine of the treatment cardiovascular and cerebrovascular disease that existing market is sold has SHUXIONG PIAN etc.; it makes Chinese patent medicine by Radix Notoginseng, Rhizoma Chuanxiong Flos Carthami three taste Chinese medicines after suitably processing; quality standard is Chinese Pharmacopoeia version in 2010; there is blood circulation promoting and blood stasis dispelling; the effect of removing obstruction in the collateral to relieve pain; for the thoracic obstruction due to stagnation of blood stasis, uncomfortable in chest, pareordia twinge that disease is seen; Angina pectoris is shown in above-mentioned patient
.
Radix Notoginseng is the dry root of araliad Panax pseudoginseng, its main component is the compounds such as Radix Notoginseng total arasaponins, flavonoid glycoside, dencichine, volatile oil and aminoacid, has hemostasis, invigorates blood circulation, enriches blood, antiinflammatory, blood fat reducing, arrhythmia, antitumor, the anti-ageing multiple pharmacological effect effect of waiting for a long time.There is the effects such as the myocardial ischemia of improvement, blood fat reducing, blood pressure lowering, antithrombotic, shock, fibrosis activity, antiinflammatory, analgesia, calmness, blood sugar lowering, defying age, enhancing immunity and hepatic cholagogic.
Rhizoma Chuanxiong is the rhizome of Umbelliferae herbaceos perennial Rhizoma Chuanxiong, is used as medicine with a long history, begins to be loaded in < < Sheng Nong's herbal classic > >, classifies as top gradely, applies very extensive.Rhizoma Chuanxiong property and flavor of peppery and warm, master returns liver, gallbladder, pericardium channel, has the effect of blood circulation promoting and blood stasis dispelling, circulation of qi promoting, wind-expelling pain-stopping.Cure mainly menoxenia due to the blood stasis stagnation of QI, dysmenorrhea amenorrhea, stagnation of QI due to depression of the liver and cause the pain in chest and hypochondrium of hematogenous blockage, headache, anemofrigid-damp arthralgia, the diseases such as treating swelling and pain by traumatic injury.The clinical disease that is mainly used in treating cardiovascular and cerebrovascular vessel, breathing, urinary system and gynecology.Its contained effective ingredient is mainly tetramethylpyazine (ligustrazine) and ferulic acid etc., still contains in addition the alkylbenzene peptides such as volatile oil, ligustilide and butylene benzene peptide.
Flos Carthami is the tubular flower of feverfew Flos Carthami (Carthamu ~ tinctoriu ~ L.), is one of Chinese medicine of main activating blood circulation to dissipate blood stasis, is usually used in vascular occlusion, traumatic injury, the skin infection card such as swell and ache.The pharmacological action of Flos Carthami is very extensive, toxicity is little, and the achievement of the diseases of cardiovascular and cerebrovascular systems such as hemiplegia of losing after treatment coronary heart disease, cerebral thrombosis, cerebral arteriosclerosis, hypertensive cerebral hemorrhage is extensively confirmed.The chemical composition of Flos Carthami is mainly divided into several large classes such as flavonoid, lignanoids, polyacetylene class, wherein flavonoid mainly comprises Carthamus yellow (saffloryellow, SY), S-A Hydroxysafflor yellow A (hydroxysaffloryellow A, HSYA) etc., be the main effective ingredient of Flos Carthami.
SHUXIONG PIAN production technology is more extensive, and Radix Notoginseng is directly used as medicine with medicinal powder, and Rhizoma Chuanxiong Flos Carthami is used as medicine after decocting in water.Due to Chinese crude drug, be subject to the impact of the environment such as the place of production, implantation methods and weather, the effective ingredient of every batch of medical material may be inconsistent, this direct medical material amount by regulation is made preparation with simple technique, index composition is not quantized, dose-effect relationship is unclear, cause the quality of every batch of product inconsistent, thereby affect pharmaceutical effectiveness.
Summary of the invention
The present invention seeks to weak point for above-mentioned SHUXIONG PIAN the composition of medicine of a kind of Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract and preparation thereof, application are provided.
The invention provides the best proportioning combination of a kind of Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract, and the pharmaceutical preparation of combination preparation thus.
The composition of medicine of a kind of Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract and preparation thereof, application are to take following technical scheme to realize:
A composition of medicine for Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract, is comprised of Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract, and wherein Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract weight proportion are 14-56:7.5-30:14-56.
Wherein, in Radix Notoginseng extract, containing total saponins, must not be less than 20.0%, in Rhizoma Chuanxiong extract, containing ferulic acid, must not be less than 0.2%, in Flos Carthami extract, hydroxyl carthamin yellow A-containing must not be less than 1 .0%.
Wherein, Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract are made after composition of medicine the amount containing total saponins, ferulic acid, S-A Hydroxysafflor yellow A in each dosage unit and are respectively following weight scope: 4.8-19.19 mg, 0.017-0.067 mg, 0.15-0.61 mg.
Wherein, Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract are made after composition of medicine the amount containing total saponins, ferulic acid, S-A Hydroxysafflor yellow A in each dosage unit and are respectively following weight than any one group in combination: 4.8:0.067:0.61,19.19:0.067:0.30,9.6:0.067:0.15,19.19:0.017:0.61.
The composition of medicine preparation method of a kind of Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract:
(1) Radix Notoginseng extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add respectively 10 times of amounts of 75% ethanol, 5 times of amounts, reflux, extract, twice, 2 hours for the first time, 1.5 hours for the second time, merge reflux extracting liquid twice, medicinal residues add 10 times of water gagings, reflux, extract, 1 hour, merge with alcohol extract, reclaim ethanol, be concentrated into thick paste, be dried, be ground into fine powder and obtain Radix Notoginseng extract;
(2) Rhizoma Chuanxiong extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 5 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, filtrate is concentrated in right amount, adds ethanol precipitate with ethanol, and making containing amount of alcohol is 65%, centrifugal, get supernatant, be concentrated into thick paste, be dried, be ground into fine powder and obtain Rhizoma Chuanxiong extract;
(3) Flos Carthami extract technological process: clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 6 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, be concentrated into thick paste, be dried, be ground into fine powder and obtain Flos Carthami extract;
(4) said extracted thing, after assay, takes respectively Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract by weight ratio according to content data, makes preparation after adding pharmaceutic adjuvant by preparation process.
Wherein, its preparation is oral formulations.
Wherein, the pharmaceutic adjuvant that contains oral formulations, can add the pharmaceutic adjuvant of oral formulations while making oral formulations.
Wherein, oral formulations comprises hard capsule, soft capsule, granule, tablet.
The thoracic obstruction of the composition of medicine of above-mentioned Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract due to preparation treatment stagnation of blood stasis, that disease is seen is uncomfortable in chest, pareordia twinge and see in the angina pectoris medicine of above-mentioned patient and apply.
What the present invention relates to is a kind of blood circulation promoting and blood stasis dispelling, medicine of removing obstruction in the collateral to relieve pain and preparation method thereof, application.1, by the effective site in Radix Notoginseng, Rhizoma Chuanxiong, flos carthami is studied, adopt 75% ethanol refluxing process to add again medicinal residues water and extract, extracted alcohol dissolubility and aqueous soluble active constituent in Radix Notoginseng.Adopt decoction and alcohol sedimentation technique to extract the alkaloids active component in Rhizoma Chuanxiong; Water extraction has extracted the flavonoid active component 2 in Flos Carthami, medical material and has made extract through above-mentioned after extracting for effective ingredient, has formulated extract simultaneously and has detected index, and worked out quality standard.3, through pharmacodynamics orthogonal experiment, the amount (that is to say the amount of ferulic acid) that shows Rhizoma Chuanxiong extract in the drug effect of each prescription group is principal element, and the onset time that resists myocardial ischemia is very fast, in general, preferred sequence is prescription 3, 9, 6 groups, be Rhizoma Chuanxiong extract top level group, when Rhizoma Chuanxiong extract adopts higher level, the amount that increases the extract of Flos Carthami has larger help (at prescription 3 to drug effect, 9, in 6 groups, the dose-effect of Flos Carthami extract becomes positive correlation), Radix Notoginseng extract also has the effect resisting myocardial ischemia, onset time is slower, played the effect that extends drug effect, but the amount of Radix Notoginseng extract and whole drug effect dependency are not obvious.Finally, by determining the optimal proportion of each extract after the test of pesticide effectiveness, so both improve the curative effect of medicine, guaranteed again the basically identical property of curative effect of every batch of finished product simultaneously.The amount of formulation effect relationship that the present invention makes is analysed clearly, definite ingredients, and the quality of the pharmaceutical preparations is stablized controlled, and curative effect is better, can meet clinical higher level demand.Through pharmacological experiment, the preparation that adopts the present invention to make has surpassed the drug effect of SHUXIONG PIAN.
The contrast of SHUXIONG PIAN and patent application composition of medicine of the present invention
The specific embodiment
By the following examples, in conjunction with results of pharmacodynamic test, further illustrate the present invention, following embodiment does not only limit the present invention for the present invention is described.
A composition of medicine preparation method for Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract, is characterized in that,
(1) Radix Notoginseng extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add respectively 10 times of amounts of 75% ethanol, 5 times of amounts, reflux, extract, twice, 2 hours for the first time, 1.5 hours for the second time, merge reflux extracting liquid twice, medicinal residues add 10 times of water gagings, reflux, extract, 1 hour, merge with alcohol extract, reclaim ethanol, be concentrated into thick paste, be dried, be ground into fine powder and obtain Radix Notoginseng extract;
(2) Rhizoma Chuanxiong extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 5 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, filtrate is concentrated in right amount, adds ethanol precipitate with ethanol, and making containing amount of alcohol is 65%, centrifugal, get supernatant, be concentrated into thick paste, be dried, be ground into fine powder and obtain Rhizoma Chuanxiong extract;
(3) Flos Carthami extract technological process: clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 6 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, be concentrated into thick paste, be dried, be ground into fine powder and obtain Flos Carthami extract;
(4) said extracted thing, after assay, takes respectively Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract by weight ratio according to content data, makes preparation after adding pharmaceutic adjuvant by preparation process.
Embodiment 1
Prescription (prescription 3):
Radix Notoginseng extract (containing total saponins 4.8mg * 1000) 14g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.61mg * 1000) 56g
Method for making: take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract by prescription, add appropriate amount of auxiliary materials mix homogeneously, obtain 1000 with capsule fill.
Embodiment 2
Prescription (prescription 3):
Radix Notoginseng extract (containing total saponins 4.8mg * 1000) 14g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.61mg * 1000) 56g
Cera Flava 45g
Gelatin is appropriate
Glycerol is appropriate
Paraffin oil is appropriate
Method for making: get Cera Flava, slight fever is dissolved it, adds Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract and paraffin wet goods proper auxiliary materials, stirs evenly, and colloid mill grinds well, decompression degasification, medicinal liquid is pressed into 1000 of soft capsules.
Embodiment 3
Prescription (prescription 3):
Radix Notoginseng extract (containing total saponins 4.8mg * 1000) 14g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.61mg * 1000) 56g
Sucrose 500g
Dextrin 250g
Making beating starch 60g
Essence 2 ml
Method for making: take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract, sucrose and the dextrin of recipe quantity, pulverized 100 mesh sieves, and fully mixed half an hour; Making beating starch is washed into 10% starch slurry, lets cool standby.Under stirring, starch slurry is joined in the material having mixed, make soft material, cross 14 mesh sieves, the wet grain of system, dry, after granulate, spray into essence, place half an hour, subpackage obtains 1000 bags.
Embodiment 4
Prescription (prescription 3):
Radix Notoginseng extract (containing total saponins 4.8mg * 1000) 14g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.61mg * 1000) 56g
Starch 150g
Microcrystalline Cellulose 65g
Carboxymethyl starch sodium 60g
Magnesium stearate 2g
Polyvidone
k30in right amount
Method for making: starch, microcrystalline Cellulose, carboxymethylstach sodium, magnesium stearate are crossed respectively 80 mesh sieves; Get PVP K30 and with anhydrous alcohol solution, make 5% solution, mix standby.By recipe quantity, take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract, starch, microcrystalline Cellulose, carboxymethyl starch sodium, mix homogeneously, adds 5% PVP K30 liquid to make soft material, cross 20 mesh sieves, the wet grain of system, after wet grain is dry, crosses 18 mesh sieves, add magnesium stearate, after mixing, tabletting, film coating, make 1000, obtain tablet.
Embodiment 5
Prescription (prescription 9):
Radix Notoginseng extract (containing total saponins 19.19mg * 1000) 56g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.30mg * 1000) 28g
Starch 160g
Microcrystalline Cellulose 50 g
Carboxymethyl starch sodium 60g
Magnesium stearate 2g
Polyvidone
k30in right amount
Method for making: starch, microcrystalline Cellulose, carboxymethylstach sodium, magnesium stearate are crossed respectively 80 mesh sieves; Get PVP K30 and with anhydrous alcohol solution, make 5% solution, mix standby.By recipe quantity, take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract, starch, microcrystalline Cellulose, carboxymethyl starch sodium, mix homogeneously, adds 5% PVP K30 liquid to make soft material, cross 20 mesh sieves, the wet grain of system, after wet grain is dry, crosses 18 mesh sieves, add magnesium stearate, after mixing, tabletting, film coating, make 1000, obtain tablet.
Embodiment 6
Prescription (prescription 6):
Radix Notoginseng extract (containing total saponins 9.6mg * 1000) 28g
Rhizoma Chuanxiong extract (containing ferulic acid 0.067mg * 1000) 30g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.15mg * 1000) 14g
Starch 120g
Microcrystalline Cellulose 65g
Carboxymethyl starch sodium 70g
Magnesium stearate 2g
Polyvidone
k30in right amount
Method for making: starch, microcrystalline Cellulose, carboxymethylstach sodium, magnesium stearate are crossed respectively 80 mesh sieves; Get PVP K30 and with anhydrous alcohol solution, make 5% solution, mix standby.By recipe quantity, take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract, starch, microcrystalline Cellulose, carboxymethyl starch sodium, mix homogeneously, adds 5% PVP K30 liquid to make soft material, cross 20 mesh sieves, the wet grain of system, after wet grain is dry, crosses 18 mesh sieves, add magnesium stearate, after mixing, tabletting, film coating, make 1000, obtain tablet.
Embodiment 7
Prescription (prescription 7):
Radix Notoginseng extract (containing total saponins 19.19mg * 1000) 56g
Rhizoma Chuanxiong extract (containing ferulic acid 0.017mg * 1000) 7.5g
Flos Carthami extract (hydroxyl carthamin yellow A-containing 0.61mg * 1000) 56g
Starch 150g
Microcrystalline Cellulose 85g
Carboxymethyl starch sodium 60g
Magnesium stearate 2g
Polyvidone
k30in right amount
Method for making: starch, microcrystalline Cellulose, carboxymethylstach sodium, magnesium stearate are crossed respectively 80 mesh sieves; Get PVP K30 and with anhydrous alcohol solution, make 5% solution, mix standby.By recipe quantity, take Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract, starch, microcrystalline Cellulose, carboxymethyl starch sodium, mix homogeneously, adds 5% PVP K30 liquid to make soft material, cross 20 mesh sieves, the wet grain of system, after wet grain is dry, crosses 18 mesh sieves, add magnesium stearate, after mixing, tabletting, film coating, make 1000, obtain tablet.
radix Notoginseng extract quality standard
differentiate:get this product powder 0. 2g, add 5, water, stir evenly, water saturated n-butyl alcohol 5ml in addition again, close plug, jolting 10 minutes, place 2 hours, centrifugal, get supernatant, add 3 times of amounts with the saturated water of n-butyl alcohol, shake up, place and make layering (centrifugal if desired), get n-butanol layer, evaporate to dryness, residue adds methanol lml to be made to dissolve, as need testing solution.Separately get ginsenoside Rb
1reference substance, ginsenoside Re's reference substance, ginsenoside Rg
1reference substance and Panax Notoginseng saponin R
1reference substance, adds methanol and makes every lm l respectively containing the mixed solution of 0.5mg, in contrast product solution.According to thin layer chromatography, test, each is put respectively on same silica gel g thin-layer plate to draw above-mentioned two kinds of solution, the 10 ℃ of following lower floor's solution placed of chloroform-ethyl acetate-methanol-water (1 5:40:22:10) of take are developing solvent, launch, take out, dry, spray, with sulfuric acid solution (1 → 10), is heated to speckle colour developing at 105 ℃ clear.In test sample chromatograph, with the corresponding position of reference substance chromatograph on, the speckle of aobvious same color; Put under ultra-violet lamp (365nm) and inspect, aobvious identical fluorescence speckle.
moisture:get this product 2 ~ 5g, put 100 ~ 105 ℃ and be dried to constant weight (double weight differential is no more than 5mg), should cross 10.0%.
assay:it is filler that chromatographic condition and system suitability be take octadecyl silicon protective embankment bonded silica gel; Take acetonitrile as mobile phase A, take water as Mobile phase B, the regulation according to the form below is carried out gradient elution; Detection wavelength is 203nm.Number of theoretical plate is pressed Panax Notoginseng saponin R
1peak calculates should be not less than 4000.
The preparation precision of reference substance solution takes ginsenoside Rg
1reference substance, ginsenoside Rb
1reference substance and Panax Notoginseng saponin R
1reference substance is appropriate, adds methanol and makes every 1ml containing ginsenoside Rg
10.4mg, ginsenoside Rb
10.4mg, Panax Notoginseng saponin R
1the mixed solution of 0.1mg, obtains.
This product powder 0.2g is got in the preparation of need testing solution, accurately weighed, and precision adds methanol 50ml, weighed weight, and placement is spent the night, put and in 80 ℃ of water-baths, keep micro-and boil 2 hours, let cool, more weighed weight, with methanol, supply the weight of less loss, shake up, filter, get subsequent filtrate, obtain.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and injection liquid chromatography, measures, and obtains.
This product is pressed dry product and is calculated, containing ginsenoside Rg
1(C
42h
72o
14), ginsenoside Rb
1(C
54h92O
23) and Panax Notoginseng saponin R
1(C
47h
80o
18) total amount must not be less than 20.0%.
?
rhizoma Chuanxiong extract quality standard
differentiate:get this product powder 0.1 g, the 20ml that adds diethyl ether, reflux 1 hour, filters, and filtrate volatilizes, and residue adds ethyl acetate 2ml to be made to dissolve, as need testing solution.Separately get Rhizoma Chuanxiong control medicinal material lg, be made in the same way of control medicinal material solution.Get again Levistilide A reference substance, add ethyl acetate and make every lml containing the solution (putting in brown measuring bottle) of 0.1mg, in contrast product solution.According to thin layer chromatography test, draw each 10 μ l of above-mentioned three kinds of solution, put in same silica gel G F respectively
254on lamellae, the just own protective embankment-ethyl acetate (3:1) of take is developing solvent, launches, and takes out, and dries, and puts under ultra-violet lamp (254nm) and inspects.In test sample chromatograph, with control medicinal material chromatograph and the corresponding position of reference substance chromatograph on, the speckle of aobvious same color.
moisture:get this product 2 ~ 5g, put 100 ~ 105 ℃ and be dried to constant weight (double weight differential is no more than 5mg), should cross 10.0%.
assay:chromatographic condition and system suitability be take octadecylsilane chemically bonded silica as filler; Methanol-1% acetum (30:70) of take is mobile phase; Detection wavelength is 321nm.Number of theoretical plate calculates and should be not less than 4000 by ferulic acid peak.
It is appropriate that ferulic acid reference substance is got in the preparation of reference substance solution, accurately weighed, puts in brown measuring bottle, adds 70% methanol and make every lml containing the solution of 20 μ g, obtains.
The about 0.2g of this product powder is got in the preparation of need testing solution, accurately weighed, puts in tool plug conical flask, precision adds people 70% methanol 50ml, close plug, weighed weight, reflux 30 minutes, lets cool, more weighed weight, the weight of supplying less loss with 70% methanol, shakes up, standing, get supernatant, filter, get subsequent filtrate, obtain.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, note people's chromatograph of liquid, measure, and obtain.
This product is pressed dry product and is calculated, containing ferulic acid (C
10h
10o
4) must not be less than 0. 2%.
flos Carthami extract quality standard
differentiate:get this product powder 0.15g, add 80% acetone soln 5ml, close plug, shakes 15 minutes, standing, gets supernatant as need testing solution.Separately get Flos Carthami control medicinal material 0.5g, be made in the same way of control medicinal material solution.According to thin layer chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, to put respectively on same silica gel H lamellae, the ethyl acetate-formic acid-water-methanol (7:2:3:0.4) of take is developing solvent, launches, and takes out, and dries.In test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, the speckle of aobvious same color.
moisture:get this product 2 ~ 5g, put 100 ~ 105 ℃ and be dried to constant weight (double weight differential is no more than 5mg), should cross 10.0%.
assay:according to high performance liquid chromatography
Chromatographic condition and system suitability be take octadecylsilane chemically bonded silica as filler; Methanol-acetonitrile-0.7% of the take acid solution (26:2:72) of seeing is mobile phase; Detection wavelength is 403nm.Number of theoretical plate calculates and should be not less than 3000 by S-A Hydroxysafflor yellow A peak.
It is appropriate that S-A Hydroxysafflor yellow A reference substance is got in the preparation of reference substance solution, accurately weighed, adds 25% methanol and make every lml containing the solution of 0.13mg, obtains.
The about 0.4g of this product powder is got in the preparation of need testing solution, accurately weighed, puts in tool plug dimension shape bottle. and precision adds 25% methanol 50ml, weighed weight, supersound process (power 300W, frequency 50kHz) 40 minutes, lets cool, weighed weight again, the weight of supplying less loss with 25% methanol, shakes up, and filters, get subsequent filtrate, obtain.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, note people's chromatograph of liquid, measure, and obtain.
This product is pressed dry product and is calculated, hydroxyl carthamin yellow A-containing (C
27h
30o
15) must not be less than 1.0%.
Radix Notoginseng extract, Rhizoma Chuanxiong extract and the research of Flos Carthami extract composition of medicine effect experiment and quadrature analysis
one. experiment purpose:
Research Radix Notoginseng extract (three kinds of proportioning 14mg, 28mg, 56mg), Rhizoma Chuanxiong extract (three kinds of proportioning 7.5mg, 15mg, 30mg) Flos Carthami extract (three kinds of proportioning 14mg, 28mg, 56mg) the Pharmacodynamic effect of various dose proportioning, for being subject to the continual exploitation of reagent that experimental basis is provided.
two. experiment material:
1. medicine and reagent:
Tested medicine: Rhizoma Chuanxiong extract, Radix Notoginseng extract, Flos Carthami extract, is dark brown pulverulent solids, the Wuxi City health bio tech ltd that is bestowed by heaven provides, and in room temperature, stores.
SHUXIONG PIAN, Zhengzhou Han Dou Pharmaceutical Group Co.,Ltd, batch number: 120903, specification: every heavy 0.25g, stores in room temperature lucifuge place.
Aspirin Enteric-coated Tablets, Baijingyu Pharmaceutical Co., Ltd., Nanjing, batch number: 120506, specification: every heavy 25mg, stores in room temperature lucifuge place.
FUFANG DANSHEN PIAN, Beijing Tongrentang Technology Development Co.ltd. Pharmaceutical Factory, batch number: 1124011, specification: every heavy 0.32g, stores in room temperature lucifuge place.
Pituitrin injection: Nanjing Xinbai Pharmaceutical Co, batch number: 130102, specification; 6U/ml, 1ml/ props up, in 4 ℃ of refrigerator cold-storages.
Sodium chloride injection: Nanjing Xiaoying Medicine Group Co.,Ltd, batch number: 2012011101, specification: 500ml:45g.
Urethane: Shanghai Qing Xi Chemical Industry Science Co., Ltd produces, lot number: 071123.
Compounding medicine:
(1) configuration of 0.5%CMC-Na: be that 5 grams of CMC-Na are added in boiling water several times, continuous stirring and dissolving, then add, dissolve, add again, dissolve, until all add, dissolve. then let cool, filter, get filtrate using, and constantly keep the skin wet in the process of heating, remain on certain level, i.e. 1000ml.Place coolingly, filter afterwards.
(2) Experimental agents configuration: before use 0.5% sodium carboxymethyl cellulose (0.5%CMC-Na) for tested medicine is ground to the even suspension solution that is mixed with desired concn.1 group of dosage: be mixed with the even suspension containing Radix Notoginseng extract 1.8mg/ml and Rhizoma Chuanxiong extract 1.0mg/ml Flos Carthami extract 1.8mg/ml.2 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 1.8mg/ml and Rhizoma Chuanxiong extract 1.9mg/ml Flos Carthami extract 3.6mg/ml.3 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 1.8mg/ml and Rhizoma Chuanxiong extract 3.9mg/ml Flos Carthami extract 7.2mg/ml.4 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 3.6mg/ml and Rhizoma Chuanxiong extract 1.0mg/ml Flos Carthami extract 3.6mg/ml.5 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 3.6mg/ml and Rhizoma Chuanxiong extract 1.9mg/ml Flos Carthami extract 7.2mg/ml.6 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 3.6mg/ml and Rhizoma Chuanxiong extract 3.9mg/ml Flos Carthami extract 1.8mg/ml.7 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 7.2mg/ml and Rhizoma Chuanxiong extract 1.0mg/ml Flos Carthami extract 7.2mg/ml.8 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 7.2mg/ml and Rhizoma Chuanxiong extract 1.9mg/ml Flos Carthami extract 1.8mg/ml.9 groups of dosage: be mixed with the even suspension containing Radix Notoginseng extract 7.2mg/ml and Rhizoma Chuanxiong extract 3.9mg/ml Flos Carthami extract 3.6mg/ml.
(3) configuration of positive drug: before use 0.5% sodium carboxymethyl cellulose (0.5%CMC-Na) for positive drug is ground to the even suspension solution that is mixed with desired concn.SHUXIONG PIAN group: be configured to the even suspension containing SHUXIONG PIAN (51.4mg/ml).Aspirin group: be configured to the even suspension containing aspirin (1mg/ml).FUFANG DANSHEN PIAN group: be configured to the even suspension containing FUFANG DANSHEN PIAN (43.5 mg/ml).
laboratory animal
Male mice in kunming, body weight 20 ± 2g, amounts to 120, is purchased from Yangzhou University's comparative medicine center; The quality certification number: SCXK(Soviet Union) 2012-0004.Experimental mouse pellet is raised, and freely drinks water.
Male SPF level SD rat, body weight 180 ± 20g, amounts to 104, is purchased from Jiangsu University's Experimental Animal Center; The quality certification number: SCXK(Soviet Union) 2009-0002.Experimental mouse pellet is raised, and freely drinks water.
key instrument
ECG-6511 type electrocardiograph: Shanghai Photoelectricity Medical electron Instrument Co., Ltd. produces.
BS 224s type electronic balance: Beijing Sai Duolisi instrument system company limited product.
three.experimental technique and result
The design of 3.1 sides of tearing open experiment
By pseudo-ginseng, Ligusticum chuanxiong Hort, flos carthami, by extraction process preparation, corresponding yield is as follows:
Pseudo-ginseng 300g → Radix Notoginseng extract (84g) (0.28g extract/g crude drug)
Ligusticum chuanxiong Hort 200 g medical material → Rhizoma Chuanxiong extracts (15g) (0.075g extract/g crude drug)
Flos carthami 250g medical material → Flos Carthami extract (70g) (0.28g extract/g crude drug)
For each test of pesticide effectiveness project, adopt orthogonal test, tentatively draft employing Three factors-levels, level adopts 50%, 100%, 200% of standard extract weight, need carry out 9 experiments.According to orthogonal table, determine that test dose is as following table 1:
Table 1 Mouse and rat test dose conversion table
Index components containing scale in each agent group amount
3.2 mice blood coagulations, anoxia enduring experiment
Get 120 of Kunming mouses, by body weight, be divided at random 12 groups, 10 every group.Be respectively negative control group, SHUXIONG PIAN group, prescription group: the 1st group, the 2nd group, the 3rd group, the 4th group, the 5th group, the 6th group, the 7th group, the 8th group, the 9th group.Positive control drug group: aspirin tablet
Each organizes dosage and drug level as following table 2:
Table 2 mice dosage and drug level
Each is organized administration volume and is 0.2ml/10g; Negative control group gives isometric 0.5%CMC-Na.Every day gastric infusion once, continuous six days.
Clotting time measuring method is glass capillary method: the glass capillary that is 1mm with internal diameter inserts mice endocanthion ball rear vein beard and gets blood.In glass capillary, blood post reaches after 10cm, fractures a bit of every 20s, checks to have or not to occur blood clotting silk.Calculating is taken a blood sample to and is occurred the time of blood clotting silk from glass capillary, is clotting time.Hypoxia endurance time measuring method: after clotting time experiment finishes, mice is positioned in 250ml wide mouthed bottle, recording start seals lid for jar until the time of mice death by suffocation in bottle is hypoxia endurance time.
Statistical procedures adopts t check analysis to process, data all with
± sd represents, P<0. 05, and P<0.01 thinks statistical significance.
The results are shown in Table 3-4.
Table 3 on the impact of clotting time of mice (
± sd)
With negative control group comparison, * P < 0.05 is significant difference * * P < 0.01 and is utmost point significant difference; With the comparison of SHUXIONG PIAN group, ﹟ P < 0.05 is significant difference, and ﹟ ﹟ P < 0.01 is utmost point significant difference
Compare with negative control group, 2 groups of dosage, 4 groups of dosage, 5 groups of dosage, 8 groups of dosage, SHUXIONG PIAN group can significant prolongation clotting times (p<0.05), 3 groups of dosage, 6 groups of dosage, 7 groups of dosage, the clotting time (p<0.01) that 9 groups of dosage and aspirin group can utmost point significant prolongation mices.With the comparison of SHUXIONG PIAN group, 9 groups of 3 groups of dosage, dosage have significant difference (p<0.05), and aspirin group has utmost point significant difference (p<0.01).(seeing the above table)
conclusion: the 3rd dosage group has extremely significant blood coagulation resisting function, and effect is better than SHUXIONG PIAN group, has significant difference (P<0.05); Be weaker than aspirin group, but unknown significance difference.Blood coagulation resisting function power is followed successively by the 9th, 7,6,5,8,4,2,1 dosage group.
Table 4 on the impact of mice hypoxia endurance time (
± sd)
Compare with negative control group: * P<0.05 is significant difference
Compare with negative control group, 3 groups of dosage, 4 groups of dosage, 5 groups of dosage, 9 groups of dosage and aspirin group can significantly improve the hypoxia endurance time (p<0.05) (seeing the above table) of mice.
conclusion: 3 groups of dosage, 4 groups of dosage, 5 groups of dosage, 9 groups of dosage and aspirin group have certain oxygen lack resistant function.
3.3 impacts on pituitrin Myocardial Ischemia Rat Ecg
Get 104 of SD rats, by body weight, be divided at random 13 groups, 8 every group.Be respectively Normal group, model control group, SHUXIONG PIAN group, prescription group: the 1st group, the 2nd group, the 3rd group, the 4th group, the 5th group, the 6th group, the 7th group, the 8th group, the 9th group.Positive control drug group: FUFANG DANSHEN PIAN 435mg/kg
Each organizes dosage and drug level as following table 5:
Table 5 rat dosage and drug level
Each is organized administration volume and is 1ml/100g; Normal group and model control group give isometric 0.5%CMC-Na.Every day gastric infusion once, continuous 6 days, 45min after last administration, by rat, with 20% urethane after the dosage intraperitoneal injection of anesthesia with 1.0g/kg, dorsal position is fixed, a separated side femoral vein, record one section normal
lead electrocardiogram, measure R-R interval, the height of J point and T ripple, then intravenous injection pituitrin 1u/kg (1U/ml), pushed away in 10 seconds, before injection (0sec) and injection afterwards 5sec, 30sec, 1min, 5min, record II lead electrocardiogram.Normal group gives isopyknic normal saline.Measure R-R interval, the height of J point and T ripple.Each each index value of time point electrocardiogram is carried out between the group of difference before and after administration to t check relatively.
3.3.1 Rat Ecg J point changing value is affected
Table 6 on Rat Ecg J point changing value (mv) impact (n=8,
± sd)
Compare with model group: * P<0.05 is significant difference, * * P<0.01 is utmost point significant difference; With the comparison of SHUXIONG PIAN group, ﹟ P < 0.05 is significant difference, and ﹟ ﹟ P < 0.01 is utmost point significant difference
Compare with model group, at 5s time point, 1 group of dosage, 2 groups of dosage, 5 groups of dosage and SHUXIONG PIAN group can significantly suppress the rising (p<0.05) that J is ordered, and 3 groups of dosage, 4 groups of dosage, 6 groups of dosage, 7 groups of dosage, 9 groups of dosage and FUFANG DANSHEN PIAN group can suppress the rising (p<0.01) that J is ordered extremely significantly; At 30s time point, 3 groups of dosage, 9 groups of dosage and FUFANG DANSHEN PIAN group can significantly suppress the lifting (p<0.05) that J is ordered; At 1 minutes point, 3 groups of dosage can significantly suppress the rising (P<0.05) that J is ordered.With the comparison of SHUXIONG PIAN group, at 5s time point, 9 groups of 3 groups of dosage, dosage have significant difference (p<0.05) and have significant difference (p<0.05), (seeing the above table) 9 groups of 3 groups of 30s time point dosage, dosage.
conclusion: the 3rd dosage group can reduce J point improvement value extremely significantly, has stronger function of resisting myocardial ischemia, effect is better than SHUXIONG PIAN group, has significant difference (P<0.05); The 3rd dosage group J point improvement value is lower than compound Salviae Miltiorrhizae group, but unknown significance difference, illustrates that the 3rd dosage group acts on to be to a certain extent better than compound Salviae Miltiorrhizae group.Function of resisting myocardial ischemia power is followed successively by the 9th, 6,7,4,5,2,1 dosage group.
3.3.2 Rat Ecg T ripple changing value is affected
Table 7 on Rat Ecg T ripple changing value (mv) impact (n=8,
± sd)
Compare with model group: * P<0.05 is significant difference, * * P<0.01 is utmost point significant difference
Model group T ripple after injection of pituitrin can obviously raise, and especially at 5s time point, raises the most obvious, and As time goes on T ripple recovers gradually.Compare with model group as can be seen from the above table, at 5s time point, 1 group of dosage, 4 groups of dosage, 5 groups of dosage, 7 groups of dosage, 8 groups of dosage, 9 groups of dosage and SHUXIONG PIAN group can significantly suppress the rising (p<0.05) of T ripple, and 3 groups of dosage, 6 groups of dosage and FUFANG DANSHEN PIAN group can suppress the rising (p<0.01) of T ripple extremely significantly; At 30s time point, 3 groups of dosage and FUFANG DANSHEN PIAN group can significantly suppress the rising (p<0.05) of T ripple, (seeing the above table).
conclusion: the 3rd dosage group can reduce T ripple improvement value extremely significantly, has stronger function of resisting myocardial ischemia, effect is better than SHUXIONG PIAN group, but have no, has significant difference.Function of resisting myocardial ischemia power is followed successively by the 6th, 9,8,7,5,4,1,2 dosage groups.
3.3.3 Rat Ecg R-R changing value is affected
Table 8 on Rat Ecg R-R interval changing value (S) impact (n=8,
± sd)
Compare with model group: * P<0.05 is significant difference, * * P<0.01 is utmost point significant difference; With the comparison of SHUXIONG PIAN group, ﹟ P < 0.05 is significant difference, and ﹟ ﹟ P < 0.01 is utmost point significant difference.
Model group R-R interval after injection of pituitrin, can obviously extend, and especially at 30s and 1m time point, extends the most obviously, and As time goes on R-R interval is recovered gradually.Compare with model group, at 5s time point, the red sheet group of 3 groups of dosage, 8 groups of dosage, 9 groups of dosage and compound recipe can significantly suppress the R-R prolongation of interval (p<0.05); At 30s time point, 1 group of dosage, 2 groups of dosage, 6 groups of dosage, 8 groups of dosage and FUFANG DANSHEN PIAN group can significantly suppress the R-R prolongation of interval (p<0.05), and 9 groups of 3 groups of dosage and dosage can extremely significantly suppress the R-R prolongation of interval (p<0.01); At 1 minutes point, 1 group of dosage, 2 groups of dosage, 5 groups of dosage, 7 groups of dosage, 8 groups of dosage, FUFANG DANSHEN PIAN group and SHUXIONG PIAN group can significantly suppress the R-R prolongation of interval (p<0.05), and 9 groups of 3 groups of dosage, 6 groups of dosage and dosage can extremely significantly suppress the R-R prolongation of interval (p<0.01); At 5 minutes points, 2 groups of dosage, 3 groups of dosage, 6 groups of dosage, 9 groups of dosage and FUFANG DANSHEN PIAN group can significantly suppress the R-R prolongation of interval (p<0.05).With the comparison of SHUXIONG PIAN group, at 30s time point, 9 groups of 3 groups of dosage, dosage have significant difference (p<0.05); At 1 minutes point, 9 groups of 3 groups of dosage, dosage have significant difference (p<0.05), (seeing the above table).
conclusion: the 3rd dosage group can shorten RR interval extremely significantly, effect is greater than SHUXIONG PIAN group, has significant difference; Effect is also greater than compound Salviae Miltiorrhizae group, but unknown significance difference.Next is the 9th, 6 groups, shortens RR interval effect also stronger.
four, conclusion (of pressure testing)
9 dosage groups of Comprehensive Experiment result have function of resisting myocardial ischemia in various degree.3 groups of (Radix Notoginseng extract 14 mg of Acute Myocardial Ischemia in Rats experiment electrocardiogram result show dose due to clotting time and pituitrin, Rhizoma Chuanxiong extract 30 mg, Flos Carthami extract 56 mg) drug effect that resists myocardial ischemia optimum, drug effect is better than SHUXIONG PIAN group and other each dosage groups, points out it to be worth further exploitation; Next is the 9th group, and the drug effect that resists myocardial ischemia is better; The the 6th and the 7th group of effect is also more excellent, but is weaker than the 3rd and the 9th dosage group; A little less than the 1st and the 2nd group of effect.
For further clear and definite Radix Notoginseng extract, Rhizoma Chuanxiong extract, the effect of Flos Carthami extract in prescription, we adopt L
9(3
4) orthogonal test analyzes the result of effect experiment, take clotting time, hypoxia endurance time, on Rat Ecg J point changing value (mv) impact, on Rat Ecg T ripple changing value (mv) impact, on Rat Ecg R-R interval changing value (S), impact is index, data statistics and interpretation of result are as follows:
Factor level table
Clotting time, hypoxia endurance time orthogonal test and data statistic
Rat Ecg J point changing value (mv) is affected to orthogonal test and data statistic
Rat Ecg T ripple changing value (mv) is affected to orthogonal test and data statistic
Rat Ecg R-R interval changing value (S) is affected to orthogonal test and data statistic
Adopt direct-vision method to align friendship interpretation of result:
Factor A(Radix Notoginseng extract)---factor A affects (5min), Rat Ecg T ripple changing value (mv) is affected (1min, 5min), on Rat Ecg R-R interval changing value (S), affects in (5s) Rat Ecg J point changing value (mv) is major influence factors, from data, when with Rhizoma Chuanxiong extract and Flos Carthami extract prescription, Radix Notoginseng extract onset time that resists myocardial ischemia is slower, later stage be take Radix Notoginseng extract as major influence factors, but the amount of Radix Notoginseng extract and drug effect dependency are little.
Factor B(Rhizoma Chuanxiong extract)---factor B is on clotting time, on Rat Ecg J point changing value (mv) impact (5s, 30s), on Rat Ecg T ripple changing value (mv) impact (5s, 30s), have appreciable impact to Rat Ecg R-R interval changing value (S) impact (30s, 1min, 5min), for major influence factors, and its level 3 is all better than other two levels, from data, Rhizoma Chuanxiong extract onset time that resists myocardial ischemia is very fast, and the increase of Rhizoma Chuanxiong extract amount, may improve drug effect.
Factor C(Flos Carthami extract)---factor C is on hypoxia endurance time, on being major influence factors in Rat Ecg J point changing value (mv) impact (1min), and overall fitting Rhizoma Chuanxiong extract helps out in drug effect.
Generally speaking, Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract are made after composition of medicine, and it is major influence factors that drug effect be take the amount of Rhizoma Chuanxiong extract, and Flos Carthami extract is minor effect factor, and the amount of Radix Notoginseng extract is without obvious dependency.
Claims (9)
1. the composition of medicine of a Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract, it is characterized in that, Radix Notoginseng extract, Rhizoma Chuanxiong extract, Flos Carthami extract, consist of, wherein Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract weight proportion are 14-56:7.5-30:14-56.
2. the composition of medicine of a kind of Radix Notoginseng extract according to claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract, it is characterized in that, in Radix Notoginseng extract, containing total saponins, must not be less than 20.0%, in Rhizoma Chuanxiong extract, containing ferulic acid, must not be less than 0.2%, in Flos Carthami extract, hydroxyl carthamin yellow A-containing must not be less than 1 .0%.
3. the composition of medicine of a kind of Radix Notoginseng extract according to claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract, it is characterized in that, Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract are made after composition of medicine the amount containing total saponins, ferulic acid, S-A Hydroxysafflor yellow A in each dosage unit and are respectively following weight scope: 4.8-19.19 mg, 0.017-0.067 mg, 0.15-0.61 mg.
4. the composition of medicine of a kind of Radix Notoginseng extract according to claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract, it is characterized in that, Radix Notoginseng extract, Rhizoma Chuanxiong extract and Flos Carthami extract are made after composition of medicine the amount containing total saponins, ferulic acid, S-A Hydroxysafflor yellow A in each dosage unit and are respectively following weight than any one group in combination: 4.8:0.067:0.61,19.19:0.067:0.30,9.6:0.067:0.15,19.19:0.017:0.61.
5. the composition of medicine preparation method of a kind of Radix Notoginseng extract claimed in claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract, is characterized in that,
(1) Radix Notoginseng extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add respectively 10 times of amounts of 75% ethanol, 5 times of amounts, reflux, extract, twice, 2 hours for the first time, 1.5 hours for the second time, merge reflux extracting liquid twice, medicinal residues add 10 times of water gagings, reflux, extract, 1 hour, merge with alcohol extract, reclaim ethanol, be concentrated into thick paste, be dried, be ground into fine powder and obtain Radix Notoginseng extract;
(2) Rhizoma Chuanxiong extract technological process: after, fragmentation clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 5 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, filtrate is concentrated in right amount, adds ethanol precipitate with ethanol, and making containing amount of alcohol is 65%, centrifugal, get supernatant, be concentrated into thick paste, be dried, be ground into fine powder and obtain Rhizoma Chuanxiong extract;
(3) Flos Carthami extract technological process: clean through selecting, weigh, put in extraction pot, add water reflux, extract, twice, add for the first time 10 times of amounts of water, extract 1.5 hours, add for the second time 6 times of amounts of water, extract 1 hour, merge reflux, extract, filtrate twice, be concentrated into thick paste, be dried, be ground into fine powder and obtain Flos Carthami extract;
(4) said extracted thing, after assay, takes respectively Radix Notoginseng extract, Rhizoma Chuanxiong extract Flos Carthami extract by weight ratio according to content data, makes preparation after adding pharmaceutic adjuvant by preparation process.
6. the composition of medicine of a kind of Radix Notoginseng extract according to claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract, is characterized in that, its preparation is oral formulations.
7. the composition of medicine of a kind of Radix Notoginseng extract according to claim 6, Rhizoma Chuanxiong extract, Flos Carthami extract, is characterized in that, the pharmaceutic adjuvant that contains oral formulations.
8. the composition of medicine of a kind of Radix Notoginseng extract according to claim 6, Rhizoma Chuanxiong extract, Flos Carthami extract, is characterized in that, oral formulations comprises hard capsule, soft capsule, granule, tablet.
9. the thoracic obstruction of the composition of medicine of a kind of Radix Notoginseng extract claimed in claim 1, Rhizoma Chuanxiong extract, Flos Carthami extract due to preparation treatment stagnation of blood stasis, that disease is seen is uncomfortable in chest, pareordia twinge and see in the angina pectoris medicine of above-mentioned patient and apply.
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| CN101744859A (en) * | 2008-12-12 | 2010-06-23 | 四川省中医药科学院中医研究所 | Pharmaceutical composition for promoting blood circulation, removing blood stasis and relieving pain and preparation method thereof |
| CN102091112A (en) * | 2009-12-11 | 2011-06-15 | 苏州世林医药技术发展有限公司 | Composition comprising carithamine and szechuan lovage rhizome total phenol active part |
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| CN102091112A (en) * | 2009-12-11 | 2011-06-15 | 苏州世林医药技术发展有限公司 | Composition comprising carithamine and szechuan lovage rhizome total phenol active part |
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