CN103616506B - 一种酶标抗体试剂 - Google Patents
一种酶标抗体试剂 Download PDFInfo
- Publication number
- CN103616506B CN103616506B CN201310670322.2A CN201310670322A CN103616506B CN 103616506 B CN103616506 B CN 103616506B CN 201310670322 A CN201310670322 A CN 201310670322A CN 103616506 B CN103616506 B CN 103616506B
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- component
- enzyme
- antibody reagent
- enzyme label
- label antibody
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- 108090000790 Enzymes Proteins 0.000 title claims abstract description 69
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 69
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 claims abstract description 27
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 21
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 16
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims abstract description 16
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910000033 sodium borohydride Inorganic materials 0.000 claims abstract description 11
- 239000012279 sodium borohydride Substances 0.000 claims abstract description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 8
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000008351 acetate buffer Substances 0.000 claims abstract description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 8
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 8
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 claims abstract description 8
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 12
- 235000011187 glycerol Nutrition 0.000 claims description 10
- 235000013905 glycine and its sodium salt Nutrition 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 7
- 230000035484 reaction time Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract description 2
- 239000004471 Glycine Substances 0.000 abstract 1
- 230000002267 hypothalamic effect Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000000427 antigen Substances 0.000 description 14
- 102000036639 antigens Human genes 0.000 description 14
- 108091007433 antigens Proteins 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 238000012360 testing method Methods 0.000 description 7
- 101710132601 Capsid protein Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 208000006454 hepatitis Diseases 0.000 description 5
- 231100000283 hepatitis Toxicity 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
- 108700039791 Hepatitis C virus nucleocapsid Proteins 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 206010029174 Nerve compression Diseases 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229940005654 nitrite ion Drugs 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000872931 Myoporum sandwicense Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
标准品浓度(pg/mL) | 丙肝核心抗原试剂盒测定OD值 | 实施例1酶结合物测定OD值 | 实施例2酶结合物测定OD值 | 实施例3酶结合物测定OD值 |
0 | 0.070 | 0.072 | 0.074 | 0.069 |
10 | 0.243 | 0.265 | 0.274 | 0.257 |
100 | 1.052 | 1.126 | 1.152 | 1.075 |
1000 | 1.695 | 1.742 | 1.813 | 1.673 |
10000 | 2.232 | 2.342 | 2.464 | 2.205 |
100000 | 2.648 | 2.769 | 2.835 | 2.603 |
相关系数 | 1 | 0.9998 | 0.9997 | 0.9999 |
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310670322.2A CN103616506B (zh) | 2013-12-11 | 2013-12-11 | 一种酶标抗体试剂 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310670322.2A CN103616506B (zh) | 2013-12-11 | 2013-12-11 | 一种酶标抗体试剂 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103616506A CN103616506A (zh) | 2014-03-05 |
CN103616506B true CN103616506B (zh) | 2015-04-15 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201310670322.2A Active CN103616506B (zh) | 2013-12-11 | 2013-12-11 | 一种酶标抗体试剂 |
Country Status (1)
Country | Link |
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CN (1) | CN103616506B (zh) |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT752102E (pt) * | 1995-01-23 | 2001-04-30 | Pasteur Sanofi Diagnostics | Conjugado imunoenzimatico seu processo de preparacao e as suas aplicacoes |
JP3260379B2 (ja) * | 1995-12-29 | 2002-02-25 | ビオテッツ ベルリン―ブッフ ゲーエムベーハー | 西洋ワサビペルオキシダーゼを用いて生体分子を標識する方法 |
CN101329339B (zh) * | 2008-07-31 | 2012-05-30 | 邹志飞 | 一种检测牛免疫球蛋白g的方法及其专用酶联免疫试剂盒 |
CN101358967B (zh) * | 2008-08-22 | 2012-07-25 | 北京望尔生物技术有限公司 | 一种检测氯丙嗪的方法及其专用酶联免疫试剂盒 |
-
2013
- 2013-12-11 CN CN201310670322.2A patent/CN103616506B/zh active Active
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CN103616506A (zh) | 2014-03-05 |
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