CN103616362A - Sensing head of fluorescent fiber sensor and preparation method of sensing head - Google Patents
Sensing head of fluorescent fiber sensor and preparation method of sensing head Download PDFInfo
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- CN103616362A CN103616362A CN201310647041.5A CN201310647041A CN103616362A CN 103616362 A CN103616362 A CN 103616362A CN 201310647041 A CN201310647041 A CN 201310647041A CN 103616362 A CN103616362 A CN 103616362A
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Abstract
The invention relates to a sensing head of a fluorescent fiber sensor. The sensing head of the fluorescent fiber sensor is of a wheel-shaped microstructure and comprises a fiber core, a coating layer and an air hole, wherein the fiber core and the coating layer are connected through tiepieces; and the inner wall of the air hole is coated with one layer of nanogold/methyl methacrylate with the thickness of 20 nm. A preparation method of the sensing head of the fluorescent fiber sensor comprises the following steps: preparing the nanogold/methyl methacrylate; coating the nanogold/methyl methacrylate on the inner wall of the air hole of the wheel-shaped microstructure fiber; and after coating is finished, roasting for 6 hours by controlling the temperature to be 50 DEG C so as to volatilize a dichloromethane solvent to obtain the sensing head of the fluorescent fiber sensor. Compared with the like products, the sensing head of the fluorescent fiber sensor has the advantages that the lower limit and the sensitivity of fluorescent detection of the sensing head can be increased by 20 times; the required sample amount is at nL amount order.
Description
Technical field
The present invention relates to a kind of sensing head, particularly sensing head of a kind of fluorescent optical fiber sensor and preparation method thereof.
Background technology
Metal surface strengthens fluorescent effect and refers to that the strong local surface plasma resonance of metal nanoparticle has strengthened metallics peripheral electromagnetic field greatly, and then changed its free space condition of fluorophor around, the effect that the fluorescence of fluorophor is enhanced, was first found by Drexhage in the seventies in last century.This effect has the fluorophor of increasing fluorescence quantum yield, increases Forster distance, improves the advantages such as fluorescent stability.As the development technique of biological detection of new generation, mark and fluorescence chemical sensor, become in recent years the focus of research.
That Fibre Optical Sensor has is highly sensitive, volume is little, quality is light, anti-electromagnetic interference (EMI), the advantage such as corrosion-resistant.The microstructured optical fibers that occur 20 end of the centurys, owing to having the incomparable excellent specific property of many traditional fiber, has become a hot fields of Fibre Optical Sensor research in recent years.
Miniature air hole in microstructured optical fibers provides the sensing passage of very advantageous, and it can make evanescent wave in optical fiber act on measured matter in longer distance.Than traditional fiber evanescent wave sensor, microstructured optical fibers does not need to remove coat and covering, and traditional fiber carries out special processing in order to improve sensitivity to the fibre core of optical fiber, above characteristic makes microstructured optical fibers evanescent wave sensor have the advantages such as simple, highly sensitive, the required sample of manufacture craft few (nL-μ L).
Summary of the invention
The object of the invention is will remove the technical matterss such as the complicated technologies such as coat and covering and the required sample size of traditional fiber evanescent wave fluorescent optical sensor large (mL-L) and sensing head of a kind of fluorescent optical fiber sensor and preparation method thereof is provided in order to improve sensitivity in order to solve traditional fiber evanescent wave fluorescent optical sensor.
Technical scheme of the present invention
A kind of sensing head of fluorescent optical fiber sensor, for wheel shape microstructured optical fibers, comprise fibre core, covering and airport, between described fibre core and covering, by 3 lacing wires, be connected, described airport inwall scribbles nm of gold/methyl methacrylate that thickness is 20nm.
The preparation method of the sensing head of above-mentioned a kind of fluorescent optical fiber sensor, specifically comprises the steps:
(1), first the nm of gold phosphate buffer of 500ul 0.1mM is poured in the dichloromethane solvent of 2ml, then the lauryl mercaptan of 300ul is poured in above-mentioned solution, use magnetic stirrer 1min, by nm of gold suction pipe sucking-off, be positioned over 6h in 75 ℃ of baking ovens and vapor away solvent, the methylene chloride of 1ml is poured in the nm of gold that vapors away solvent, solution takes on a red color and illustrates that nm of gold has been dissolved in organic solvent dichloromethane, obtains the methylene chloride that contains nm of gold again;
Finally methyl methacrylate is dissolved in this methylene chloride that contains nm of gold, the concentration of controlling methyl methacrylate is 1mg/ml, obtains nm of gold/methyl methacrylate solution;
(2), nm of gold/methyl methacrylate solution of 2ml is poured in 4ml vial, vial is positioned in airtight container, in the middle of the lid of container, there is a rubber plug, with injection needle, sting rubber plug, hole by wheel shape microstructured optical fibers through injection needle is placed in the vial that nm of gold/methyl methacrylate solution is housed, and one end end face that makes wheel shape microstructured optical fibers submerges in nm of gold/methyl methacrylate solution, open the nitrogen cylinder of closed container link, pressure is set to 10psi, after 30 seconds, can see liquid from the airport of wheel shape microstructured optical fibers other end end face out, continue 5 minutes, nm of gold/methyl methacrylate can be coated to the inwall of the airport of wheel shape microstructured optical fibers, after having applied, in baking oven, controlling temperature is that 50 ℃ of baking 6h are to vapor away dichloromethane solvent, obtain the sensing head of fluorescent optical fiber sensor.
The sensing head of above-mentioned a kind of fluorescent optical fiber sensor, for detection of nitro arene explosive substance, it detects lower limit and sensitivity and can improve 20 times compared with similar products, and required sample size is few, is nL magnitude.
Beneficial effect of the present invention
The sensing head of a kind of fluorescent optical fiber sensor of the present invention, because strengthening fluorescent effect, nm of gold surface can make the fluorescence intensity of fluorescence indicator increase by tens times even higher, and can make fluorescence lifetime reduce, improve the light stability of fluorescence indicator, thereby improve sensitivity and the sensor stability detecting.
Further, the sensing head of a kind of fluorescent optical fiber sensor of the present invention, due to the longer the interaction distance of the stronger evanescent wave field of wheel shape microstructured optical fibers and test substance and evanescent wave field, the surface of combining nano gold strengthens the sensitivity that fluorescent effect can further improve detection.Compared with similar products, airport inwall is coated with the wheel shape microstructure optical fiber sensing head of nm of gold/methyl methacrylate fluoroscopic examination lower limit and sensitivity can improve 20 times.
Further, the sensing head of a kind of fluorescent optical fiber sensor of the present invention, because airport is of a size of micron dimension, if be 6 μ m with airport diameter, length be the microstructured optical fibers of 16cm as sensing head, required sample size only has nL magnitude, has reduced required sample size.
In sum, the sensing head of a kind of fluorescent optical fiber sensor of the present invention combines the advantage that metal surface strengthens fluorescent effect and microstructured optical fibers, utilize metal surface to strengthen fluorescent effect and improve fluorescent optical fiber sensor sensitivity and detect lower limit, reduce the sensing head of required sample size.
Accompanying drawing explanation
The structural representation of the sensing head of Fig. 1, a kind of fluorescent optical fiber sensor.
Embodiment
Below by specific embodiment, also by reference to the accompanying drawings the present invention is further set forth, but do not limit the present invention.
embodiment 1
A kind of sensing head of fluorescent optical fiber sensor, its structural representation as shown in Figure 1, for wheel shape microstructure, comprise fibre core 1, covering 3 and airport 2, between described fibre core 1 and covering 3, by 3 lacing wires, be connected, it is 20nm nm of gold/methyl methacrylate that described airport 2 inwalls scribble a layer thickness.
The preparation method of the sensing head of above-mentioned a kind of fluorescent optical fiber sensor, specifically comprises the steps:
(1), first the nm of gold phosphate buffer of 500ul0.1 mM is poured in the dichloromethane solvent of 2ml, then the lauryl mercaptan of 300ul is poured in above-mentioned solution, use magnetic stirrer 1min, by nm of gold suction pipe sucking-off, be positioned over 6h in 75 ℃ of baking ovens and vapor away solvent, the methylene chloride of 1ml is poured in the nm of gold that vapors away solvent, solution takes on a red color and illustrates that nm of gold has been dissolved in organic solvent dichloromethane, obtains the methylene chloride that contains nm of gold again;
Finally methyl methacrylate is dissolved in this methylene chloride that contains nm of gold, the concentration of controlling methyl methacrylate is 1mg/ml, obtains nm of gold/methyl methacrylate solution;
(2), nm of gold/methyl methacrylate solution of the above-mentioned gained of 2ml is poured in 4ml vial, vial is positioned in airtight container, in the middle of the lid of container, there is a rubber plug, with injection needle, sting rubber plug, by airport diameter, be 6 μ m, length is that the wheel shape microstructured optical fibers of 16cm is placed in the vial that nm of gold/methyl methacrylate solution is housed through the hole of injection needle, and to make airport diameter be 6 μ m, length is one end end face of the wheel shape microstructured optical fibers of 16cm nm of gold/methyl methacrylate solution that submerges, open the nitrogen cylinder of closed container link, pressure is set to 10psi, after 30 seconds, can see that liquid is 6 μ m from airport diameter, length be in the airport of wheel shape microstructured optical fibers other end end face of 16cm out, continue 5 minutes, nm of gold/methyl methacrylate can be coated to airport diameter is 6 μ m, length is the inwall of airport of the wheel shape microstructured optical fibers of 16cm, after having applied, in baking oven, controlling temperature is that 50 ℃ of baking 6h are to vapor away dichloromethane solvent, obtain the sensing head of fluorescent optical fiber sensor.
application Example
By the sensing head of a kind of fluorescent optical fiber sensor of above-described embodiment 1, for detection of nitro arene explosive substance, its concrete steps are as follows:
First, will the fluorescence indicator of explosive sensitivity be coated on to airport inwall;
Then, utilize the light of 488nm as light source, the airport diameter that the light end face that light source sends is coupled into the sensing head of fluorescent optical fiber sensor is 6 μ m, length is in the microstructured optical fibers fibre core of 16cm, airport diameter is 6 μ m, length is that to put into concentration be 6.3ppm explosive solution to the other end of the microstructured optical fibers of 16cm, due to kapillary suction-operated, it is 6 μ m that explosive will enter airport diameter, length is in the airport of microstructured optical fibers of 16cm, fluorescence indicator will produce fluorescence at the exciting light of 488nm, fluorescence intensity can reduce along with the increase of explosive concentration, with spectrometer, survey fluorescence intensity and then carry out the detection of explosive concentration.
Its sensitivity and detection lower limit are respectively 1ppm and 6.3ppm, above-mentioned testing result shows, airport diameter is 6 μ m, microstructured optical fibers length is the sensing head of the fluorescent optical fiber sensor of 16cm, and the volume of required sample is about 27nL can reach very high sensitivity and the very low inspection lower limit of content.
comparative examples
By like product, be that common single-mode fiber is removed and applied after covering the fluorescence indicator of explosive sensitivity as sensing head for detection of nitro arene explosive substance, its concrete steps are as follows:
First, this sensing head is positioned in the explosive solution of 63ppm;
Then, utilize the light of 488nm as light source, the light end face that light source sends is coupled in fiber core, fluorescence indicator will produce fluorescence at the exciting light of 488nm, fluorescence intensity can reduce along with the increase of explosive concentration, with spectrometer, surveys fluorescence intensity and then carries out the detection of explosive concentration.
Its sensitivity and detect lower limit and be respectively 10ppm and 63ppm, above-mentioned testing result shows, and common single-mode fiber is removed and is applied after covering the fluorescence indicator of explosive sensitivity as sensing head, and the volume of required sample is about 1mL.
Above-mentioned Application Example and comparative examples are analyzed, result shows, the sensing head of a kind of fluorescent optical fiber sensor of the present invention, compared with similar products, fluoroscopic examination lower limit and sensitivity that airport inwall is coated with the wheel shape microstructure optical fiber sensing head of nm of gold/methyl methacrylate can improve 20 times, and required sample size is few.
Foregoing is only the basic explanation of the present invention under conceiving, and according to any equivalent transformation that technical scheme of the present invention is done, all should belong to protection scope of the present invention.
Claims (3)
1. the sensing head of a fluorescent optical fiber sensor, for wheel shape microstructure, comprise fibre core, covering and airport, between fibre core and covering, by 3 lacing wires, be connected, it is characterized in that described airport inwall scribbles nm of gold/methyl methacrylate that thickness is 20nm.
2. the preparation method of the sensing head of a kind of fluorescent optical fiber sensor as claimed in claim 1, is characterized in that, specifically comprises the steps:
(1), first the nm of gold phosphate buffer of 500ul 0.1 mM is poured in the dichloromethane solvent of 2ml, then the lauryl mercaptan of 300ul is poured in above-mentioned solution, use magnetic stirrer 1min, by nm of gold suction pipe sucking-off, be positioned over 6h in 75 ℃ of baking ovens and vapor away solvent, the methylene chloride of 1ml is poured in the nm of gold that vapors away solvent, solution takes on a red color and illustrates that nm of gold has been dissolved in organic solvent dichloromethane, obtains the methylene chloride that contains nm of gold again;
Finally methyl methacrylate is dissolved in this methylene chloride that contains nm of gold, the concentration of controlling methyl methacrylate is 1mg/ml, obtains nm of gold/methyl methacrylate solution;
(2), nm of gold/methyl methacrylate solution of 2ml is poured in 4ml vial, vial is positioned in airtight container, in the middle of the lid of container, there is a rubber plug, with injection needle, sting rubber plug, hole by wheel shape microstructured optical fibers through injection needle is placed in the vial that nm of gold/methyl methacrylate solution is housed, and one end end face that makes wheel shape microstructured optical fibers nm of gold/methyl methacrylate solution that submerges, open the nitrogen cylinder of closed container link, pressure is set to 10psi, after 30 seconds, can see liquid from the airport of wheel shape microstructured optical fibers other end end face out, continue 5 minutes, nm of gold/methyl methacrylate can be coated to the inwall of the airport of wheel shape microstructured optical fibers, after having applied, controlling temperature is 50 ℃ of baking 6h, obtain the sensing head of fluorescent optical fiber sensor.
3. the preparation method of the sensing head of a kind of fluorescent optical fiber sensor as claimed in claim 2, is characterized in that the airport diameter of described wheel shape microstructured optical fibers is 6 μ m, and length is 16cm.
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Cited By (4)
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| CN103954377A (en) * | 2014-04-17 | 2014-07-30 | 深圳大学 | Temperature sensor based on microstructure fibers, manufacturing method of temperature sensor, and temperature measuring device |
| CN104931688A (en) * | 2015-04-30 | 2015-09-23 | 南开大学 | Microstructure fiber biochip and manufacturing method thereof |
| CN110132322A (en) * | 2019-04-08 | 2019-08-16 | 东莞理工学院 | A kind of ultraviolet irradiation enhanced fiber sensor and preparation method thereof |
| WO2020214088A1 (en) * | 2019-04-17 | 2020-10-22 | Agency For Science, Technology And Research | Optical fiber for sensing an analyte, methods of forming and using the same |
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| CN102879376A (en) * | 2012-10-22 | 2013-01-16 | 上海电力学院 | Sensor for detecting nitro-aromatics explosives |
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| CN101975880A (en) * | 2010-09-08 | 2011-02-16 | 中国科学院电工研究所 | Optical current transformer sensing head and sensing method |
| CN102680431A (en) * | 2012-05-25 | 2012-09-19 | 浙江大学 | Method for exciting surface plasmon resonance of metal nanoparticle by nano-optical fiber guide wave |
| CN102879376A (en) * | 2012-10-22 | 2013-01-16 | 上海电力学院 | Sensor for detecting nitro-aromatics explosives |
Non-Patent Citations (5)
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954377A (en) * | 2014-04-17 | 2014-07-30 | 深圳大学 | Temperature sensor based on microstructure fibers, manufacturing method of temperature sensor, and temperature measuring device |
| CN104931688A (en) * | 2015-04-30 | 2015-09-23 | 南开大学 | Microstructure fiber biochip and manufacturing method thereof |
| CN104931688B (en) * | 2015-04-30 | 2019-05-17 | 南开大学 | A kind of microstructured optical fibers biochip and preparation method thereof |
| CN110132322A (en) * | 2019-04-08 | 2019-08-16 | 东莞理工学院 | A kind of ultraviolet irradiation enhanced fiber sensor and preparation method thereof |
| WO2020214088A1 (en) * | 2019-04-17 | 2020-10-22 | Agency For Science, Technology And Research | Optical fiber for sensing an analyte, methods of forming and using the same |
| US20220187532A1 (en) * | 2019-04-17 | 2022-06-16 | Agency For Science, Technology And Research | Optical fiber for sensing an analyte, methods of forming and using the same |
| EP3956703A4 (en) * | 2019-04-17 | 2022-12-21 | Agency for Science, Technology and Research | Optical fiber for sensing an analyte, methods of forming and using the same |
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Application publication date: 20140305 |