CN103614473B

CN103614473B - Preparation method and applications of cDNA chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin

Info

Publication number: CN103614473B
Application number: CN201310609364.5A
Authority: CN
Inventors: 曾玲; 姜建军; 陆永跃; 何晓芳; 梁广文; 温硕洋
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2013-11-27
Filing date: 2013-11-27
Publication date: 2015-01-21
Anticipated expiration: 2033-11-27
Also published as: CN103614473A

Abstract

The invention discloses a preparation method and applications of a cDNA (complementary Deoxyribonucleic Acid) chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin. Upregulated drug resistance specificity expression gene segments of different resistant strains of bactrocera dorsalis can be obtained respectively by utilizing a forward suppression subtractive hybridization method, the related drug-resistant specificity expression genes are subjected to PCR (Polymerase Chain Reaction) amplification, 118 related resistance specificity gene segments of bactrocera dorsalis resistant to trichlorphon and 103 related resistance specificity gene segments of bactrocera dorsalis resistant to beta-cypermethrin can be obtained through purifying and recovering. The cDNA chip for detecting the resistance of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin can be constructed by taking the specific resistant target gene segments as DNA probes. The cDNA chip can simply and effectively realize the real-time detection on the drug resistance level of bactrocera dorsalis.

Description

The anti-Trichlorphon of citrus fruit fly and effective cypermethrin Detection of insecticide resistance detect preparation method and the application thereof of cDNA chip

Technical field

The present invention relates to insect resistance detection technique field.More specifically, relate to and a kind ofly detect cDNA chip of the resistance level of the anti-Trichlorphon of citrus fruit fly and effective cypermethrin and its preparation method and application.

Background technology

The definition of the World Health Organization in nineteen fifty-seven to insect resistance is: " insect has the ability the phenomenon grown up in its population that tolerate and kill the dose of most of individuality in normal population ".The resistance of insect insecticide is a kind of evolutionary phenomena, it is the external manifestation that insect population internal genetic structure continues change under sterilant selective action, be a kind of characteristic of insect population, mainly comprise natural tolerance to drugs, mutual resistance, negative mutual resistance and multidrug resistance etc.The consequence that insect develops immunity to drugs is: existing Agro-chemicals control dose effect reduction-people start to strengthen drug dose-insect-resistant enhancing and finally cause preventing and treating unsuccessfully, bring serious destructive consequence to environment and human food prods's safety in production.Citrus fruit fly is one of important pests on China south China and southwest fruit vegetable, every year to I cross agriculture production particularly economic fruit cause great financial loss.At present, the Agro-chemicals control that field control citrus fruit fly mainly adopts, field population monitoring data presentation citrus fruit fly for many years creates resistance to Multiple Pesticides.

In order to delay insect resistance development; reduce the use of agricultural chemicals dosage, protection of the environment and human security, monitor insect resistance; its resistance of real-time grasp is dynamic, and timely rotation uses dissimilar agricultural chemicals to be that the one that improvement insect resistance produces is well tactful.The mechanism of insect resistance is mainly divided into: behavior resistance, target resistance and physiological metabolism resistance.A lot of research proves that these drug resistance mechanism of insect are all by gene regulating, and gene and resistance are the relations of a kind of internal cause-apparent.Therefore, under understanding dissimilar sterilant effect, when insect produces resistance, the expression of its body internal specific resistance related gene, can provide foundation for understanding insect resistance development level.

Gene chip hybridization technology (gene chip hybrid analysis) develops based in situ hybridization principle, according to the principle of base complementrity, utilizes gene probe to identify specific gene in gene compound.A large amount of DNA probe molecule is fixed on upholder by this technology, then hybridizes with fluorescently-labeled sample (DNA or RNA), carrys out the quantity of judgement sample target by detecting hybridization signal power.The cDNA produced after mRNA in the cell derived under different individualities, tissue, cell cycle, etap, differential period, pathology, stimulation or reverse transcription can be detected, thus to the individual specificity of these genetic expressions, tissue specificity, etap specificity, differential period specificity, pathology specificity, stimulate specificity to carry out comprehensive analysis and judgement, rapidly one or several gene and correlative factor are connected, accelerate the determination of these gene functions, have broad application prospects.Utilize expressivity gene chip hybridization technology, thousands of the different genes that can simultaneously resist in the property of medicine and sensitive insect body carry out differential expression detection by quantitative.

Citrus fruit fly is one of important pests on China south China and southwest fruit vegetable.In agriculture production, in order to reduce the loss, at present, the Agro-chemicals control that field control citrus fruit fly mainly adopts, the field population monitoring data presentation citrus fruit fly for many years Multiple Pesticides such as Trichlorphon, effective cypermethrin creates resistance.2002-2004, researcher adopts medicine embrane method to determine 4 provinces (district) citrus fruit flies such as Guangdong, Hainan, Fujian, Guangxi b. dorsalispopulation is to the resistance of Trichlorphon and effective cypermethrin, result shows Chenghai, the south of Fujian Province citrus fruit fly population reaches 4.56 and 4.07 times to Trichlorphon resistance, most area citrus fruit fly population is still in sensitive stage to above-mentioned 2 kinds of insecticide resistances, monitor the resistance of 2003-2004 Suburb of Guangzhou citrus fruit fly adult to Trichlorphon and effective cypermethrin to change simultaneously, find except Trichlorphon, the resistance change of citrus fruit fly to effective cypermethrin is not obvious.Continue to monitor the field citrus fruit fly development of drug resistance of different areas, China 7 provinces between ensuing 2005-2007, result shows that different areas citrus fruit fly is all in rising trend to above-mentioned 2 kinds of medicament resistance.The continuous monitoring of 2008-2012 to national different areas subsequently shows that field citrus fruit fly population resists even high resistance level in all reaching above-mentioned 2 kinds of drug resistance.

In order to delay insect resistance development; reduce the use of agricultural chemicals dosage, protection of the environment and human security, monitor insect resistance; its resistance of real-time grasp is dynamic, and timely rotation uses dissimilar agricultural chemicals to be that the one that improvement insect resistance produces is well tactful.

What the monitoring for resistance of current citrus fruit fly mainly adopted is bioassay method, but traditional bioassay method is more loaded down with trivial details, operate more complicated, each mensuration all needs a large amount of worm sources, often need first to take back hand-feeding propagation from field acquisition part worm source to reach for examination borer population, result makes the Detection of insecticide resistance recorded have hysteresis quality.In addition, biological assay is comparatively large by the impact of external environment (as: worm state, nutrition, temperature, humidity etc.), repeatability and accuracy lower.And molecular biology monitoring for resistance technology, its sensitivity is higher, only just can resist property of medicine degree with a small amount of examination worm to detect, can realize detecting the high-flux parallel of sample, simple to operate, simple to operate, to save time and testing error is little, can determine the resistance degree of insect individuality, this point is that bioassay method is beyond one's reach.And realize molecule monitoring for resistance and will resist property of medicine gene and fully understand, detecting more Genes relating to drug resistance could reflect development of drug resistance level more accurately.

Insect resistance mechanism is the result of its vivo gene regulation and control, although investigators inquire into from many aspects the drug resistance mechanism of citrus fruit fly, also there are some problems, less in the research of resistance target gene, only relates to acewith alpha 6gene, has also only carried out preliminary study to indivedual enzyme or albumen variation phenomenon in the correlative study of metabolism resistance, does not relate to the gene studies of its coding.Insect resistance is the result of the common coordinative role of polygene simultaneously, is a kind of multigentic effect phenomenon.But have that how many genes participate in resistance effect on earth, to relate to the whether identical and drug resistance gene of drug resistance gene be that the research how participating in the aspects such as resistance process still lacks report to different Detection of insecticide resistances.

Summary of the invention

Technical problem to be solved by this invention overcomes the drug-fast technical deficiency of existing detection citrus fruit fly, provides a kind of cDNA chip detecting the resistance related gene expression level of the anti-Trichlorphon of citrus fruit fly and effective cypermethrin.

Another object of the present invention is to the construction process that above-mentioned cDNA chip is provided.

The present invention also has a goal of the invention to be to provide the application of said chip.

Goal of the invention of the present invention is achieved by the following technical programs:

There is provided a kind of cDNA chip detecting the anti-Trichlorphon of citrus fruit fly and effective cypermethrin resistance level, described chip comprises 221 target gene cDNA probes; Wherein 118 target gene DNA probes are citrus fruit fly anti-Trichlorphon resistance relative specific gene fragment, and 103 target gene DNA probes are citrus fruit fly anti-effective cypermethrin resistance relative specific gene fragment.

Wherein, 118 citrus fruit fly anti-Trichlorphon specific gene fragments are: S004 (4)-A9, S004 (4)-B7, S004 (4)-D6, S004 (4)-H9, S004 (5)-B10, S004 (5)-B5, S004 (5)-C10, S004 (5)-C7, S004 (5)-D1, S004 (5)-D10, S004 (5)-D11, S004 (5)-G1, S004 (5)-H3, S004 (5)-H7, S004 (6)-10, S004 (6)-12, S004 (6)-18, S004 (6)-21, S004 (7)-100, S004 (7)-109, S004 (7)-138, S004 (7)-156, S004 (7)-165, S004 (7)-183, S004 (8)-197, S004 (8)-199, S004 (8)-217, S004 (8)-225, S004 (8)-240, S004 (8)-248, S004 (9)-293, S004 (9)-308, S004 (9)-328, S004 (10)-394, S004 (10)-401, S004 (10)-443, S004 (10)-471, S005 (1)-A1, , S005 (1)-B2, , S005 (1)-C1, S005 (1)-D12, S005 (1)-G8, S005 (2)-A8, S005 (2)-C7, S005 (2)-E8, S005 (2)-G1, S005 (3)-A5, S005 (3)-A9, S005 (3)-A11, S005 (3)-C11, S005 (3)-D12, S005 (3)-D8, S005 (3)-H12, S005 (4)-A2, S005 (4)-A3, S005 (4)-A8, S005 (4)-B8, S005 (4)-G1, S005 (4)-G2, S005 (5)-A5, S005 (5)-B7, S005 (5)-E11, S005 (5)-G6, S005 (5)-G9, S005 (5)-H2, S005 (6)-13, S005 (6)-17, S005 (6)-49, S005 (7)-98, S005 (7)-100, S005 (7)-103, S005 (7)-116, S005 (7)-107, S005 (7)-131, S005 (7)-176, S005 (7)-178, S005 (7)-181, S005 (7)-188, S005 (7)-189, S005 (8)-200, S005 (8)-194, S005 (8)-211, S005 (8)-212, S005 (8)-213, S005 (8)-216, S005 (8)-224, S005 (8)-235, S005 (8)-236, S005 (8)-254, S005 (8)-259, S005 (8)-261, S005 (8)-286, S005 (8)-264, S005 (9)-293, S005 (9)-299, S005 (9)-304, S005 (9)-308, S005 (9)-310, S005 (9)-312, S005 (9)-317, S005 (9)-329, S005 (9)-333, S005 (9)-350, , S005 (9)-355, S005 (9)-357, S005 (9)-358, S005 (9)-359, S005 (9)-371, S005 (10)-388, S005 (10)-390, S005 (10)-401, S007 (3)-G11, S007 (6)-34, S007 (7)-107, S007 (7)-178, S007 (8)-192, S007 (8)-259, S007 (10)-420, sequence is as shown in SEQ ID NO.5 ~ SEQ ID NO.122.

103 citrus fruit fly anti-effective cypermethrin specific gene fragments are: S002 (1)-A3, S002 (1)-B4, S002 (3)-D9, S002 (3)-E7, S002 (4)-C10, S002 (4)-E4, S002 (4)-F8, S002 (5)-E5, S002 (6)-16, S002 (6)-19, S002 (6)-82, S002 (8)-270, S002 (10)-416, S002 (10)-452, S002 (10)-444, S008 (1)-B2, S008 (1)-D11, S008 (1)-D5, S008 (1)-D6, S008 (1)-E3, S008 (1)-F10, S008 (1)-F12, S008 (2)-B1, S008 (2)-F10, S008 (2)-F12, S008 (2)-G1, S008 (3)-B10, S008 (3)-C11, S008 (3)-D10, S008 (5)-B12, S008 (5)-B8, S008 (5)-C7, S008 (5)-E5, S008 (5)-G11, S008 (5)-H2, S008 (6)-14, S008 (6)-29, S008 (6)-42, S008 (6)-58, S008 (6)-64, S008 (6)-77, S008 (6)-85, S008 (6)-93, S008 (7)-130, S008 (7)-135, S008 (8)-276, S003 (1)-A10, S003 (1)-B6, S003 (1)-D7, S003 (1)-E1, S003 (1)-E9, S003 (1)-F2, S003 (1)-F5, S003 (1)-F9, S003 (1)-G4, S003 (2)-C6, S003 (2)-D4, S003 (2)-D5, S003 (2)-E7, S003 (2)-F3, S003 (2)-G5, S003 (3)-F1, S003 (3)-H10, S003 (4)-A8, S003 (4)-C1, S003 (4)-C12, S003 (4)-F1, S003 (4)-G6, S003 (5)-B11, S003 (5)-B2, S003 (5)-B8, S003 (5)-E6, S003 (5)-H5, S003 (6)-8, S003 (6)-32, S003 (6)-51, S003 (6)-74, S003 (6)-81, S003 (6)-92, S003 (7)-151, S003 (7)-160, S003 (7)-165, S003 (7)-167, S003 (7)-175, S003 (8)-196, S003 (8)-204, S003 (8)-220, S003 (8)-224, S003 (8)-231, S003 (8)-249, S003 (8)-271, S003 (8)-284, S003 (8)-288, S003 (9)-311, S003 (9)-312, S003 (9)-321, S003 (9)-352, S003 (10)-395, S003 (10)-404, S003 (10)-405, S003 (10)-406, S003 (10)-432, S003 (10)-469, sequence is as shown in SEQ ID NO.123 ~ SEQ ID NO.225.

The construction process of cDNA chip provided by the invention is as follows:

S1.SSH library construction: the RNA extracting citrus fruit fly sensitive strain, Trichlorphon resistant strain and effective cypermethrin resistant strain respectively, strategy and the method (Li in SSH library is built by this laboratory, 2011), the SSH library relevant with anti-effective cypermethrin to anti-Trichlorphon is built respectively;

S2. the poor loss of weight group clone of difference random choose 2820 from two libraries that S1 builds, then shakes bacterium, extracts recombinant plasmid dna;

S3. respectively with S2 obtain recombinant plasmid dna for template, with adapter-primer Nested PCR Primer 1F(SEQ ID NO.1) and Nested PCR Primer 2R(SEQ ID NO.2) carry out pcr amplification, by the unicity of each its concentration of gene PCR product detected through gel electrophoresis and fragment, and carry out purifying recovery;

S4. adopted by S3 purified product chip point sample instrument point sample on the slide glass carrier of chemically modified, the positive and negative control are set simultaneously, obtain initial gene chip;

S5. adopt the resistant strain total serum IgE used when building SSH library and sensitive strain total serum IgE for detecting sample respectively, DNA chip hybridization is carried out to the initial gene chip that S4 obtains, remove false positive differential gene, the sample selecting hybridization rear rise signal value on gene chip to be more than or equal to 2 is resistance relevant difference expressing gene;

The recombinant plasmid of the resistance relevant difference expressing gene S6. obtained with S5 carries out pcr amplification for template, primer is with described in S3, purifying recovery is carried out to product and checks order, sequence adopts DNAstar software to carry out splicing assembling, remove and repeat and miscellaneous sequence, picking goes out single non-overlapping sequence as resistance specified associated target gene.

S7. the resistance specified associated target gene obtained by S6 utilizes gene chip sample applying instrument point sample on the sheet glass carrier of chemically modified, the positive and negative control are set simultaneously, entrust Beijing Boao Biological Co., Ltd to build the cDNA chip of the resistance level detecting the anti-Trichlorphon of citrus fruit fly and anti-effective cypermethrin.

Wherein, the concrete operations of SSH library construction described in step S1 comprise joint connection, for the first time subtractive hybridization, second time subtractive hybridization, first time inhibition PCR, second time inhibition PCR; Citrus fruit fly house-keeping gene G6PDH is utilized to carry out semiquantitive PCR, detect described joint respectively to connect and subtractive hybridization efficiency, primer sequence is G6PDH-F:5 '-GCTGCCGAAACCTACAAA-3 ' (SEQ ID NO.3), G6PDH-R:5 '-CGCTTATCATAGCCACCC-3 ' (SEQ ID NO.4), does not contain in amplified fragments rsa Irestriction enzyme site.

The sheet glass carrier of chemically modified described in step S7 refers to: on carrier surface, introduce amido functional group by chemical reaction, enables DNA and surface functional group energy covalent attachment, stably solidifies in support surface.

The present invention has following beneficial effect:

The present invention adopts biochip technology first, excavates and finds citrus fruit fly Genes relating to drug resistance fragment, then utilizes resistance related gene fragment point to make specificity target resistance Related cDNAs chip, monitors citrus fruit fly resistance level.Thousands of the different genes that can simultaneously resist in the property of medicine and responsive polypide carry out differential expression detection by quantitative, process is simply effective, compensate for the deficiency of traditional sod cultivation, realize high-throughput, quick, accurate Real-Time Monitoring citrus fruit fly resistance development, thus delay its resistance, realize foodstuff production safety.

In addition, detection respectively by antagonism 3.21 times, Trichlorphon, 16.8 times and 69.5 times and anti-effective cypermethrin 5.3 times, 21.6 times and 160.9 times citrus fruit fly adult strain resistance related gene expression levels shows, this gene chip has high-throughput, integrated and microminiaturized advantage.

Accompanying drawing explanation

Fig. 1 is initial gene cDNA microarray SSH clone library hybrid screens figure.

Fig. 2 is that the present invention prepares cDNA chip detection citrus fruit fly resistant strain hybrid screens figure.

Embodiment

Further illustrate the present invention below in conjunction with specific embodiment, unless stated otherwise, the reagent that the present invention adopts, equipment and experimental technique are the art conventional reagent, equipment and method.

The test kit (purchased from Guangzhou Rui Zhen biotech firm, concrete operations are carried out with reference to Li Yi peak, this laboratory Ph D dissertation) that embodiment of the present invention subtractive hybridization (SSH) adopts Clontech company to produce; Pcr amplification reagent, competent cell DH5 α are purchased from Rui Zhen biotech firm, and total RNA extraction reagent Trizol is purchased from Invitrogen company, and cloning vector pGEM-T is purchased from Promega company.

Molecular biology elementary operation: the recovery of agarose gel electrophoresis, PCR primer purifying, clone's connection, conversion, plasmid extraction, enzyme cut qualification equimolecular biologic operation according to " Molecular Cloning: A Laboratory operational guidance " (Science Press, the third edition, Huang Peitang compiles, 2002) operation.Sequence has Shanghai Invitrogen Corp. Guangzhou Branch to complete.

embodiment 1 detects the structure of the cDNA chip of the anti-Trichlorphon of citrus fruit fly and effective cypermethrin resistance level

1, the structure in the anti-Trichlorphon of citrus fruit fly and effective cypermethrin resistant strain SSH library

(1) citrus fruit fly Selection of resistant strain:

The parental generation F of sensitive strain as resistance Screening of Strains Against of reservation is raised with testing laboratory ₀, raise under indoor identical conditions, adopt medicine embrane method, carry out by generation screening with Trichlorphon and effective cypermethrin respectively.

Sensitive strain F ₀choice criteria: Trichlorphon is to the median lethal concentration(LC&-{50}) LC of citrus fruit fly adult sensitive strain ₅₀=2.65mg/L; Effective cypermethrin is to the median lethal concentration(LC&-{50}) LC of citrus fruit fly sensitive strain ₅₀=3.36mg/L.

The acquisition of resistant strain: after the screening of some algebraically, Trichlorphon screening is to 22 generations, and Trichlorphon is to the median lethal concentration(LC&-{50}) LC of citrus fruit fly adult ₅₀=184.17mg/L; Effective cypermethrin screening is to 24 generations, and effective cypermethrin is LC to the median lethal concentration(LC&-{50}) of citrus fruit fly adult ₅₀=538.49mg/L.

Concrete screening step is: first measure parental generation (F ₀) virulence base-line sensitivities to Trichlorphon and effective cypermethrin, then adopt medicine embrane method, with median lethal concentration(LC&-{50}) (LC ₅₀) resistance screening is carried out to population; With acetone diluted medicament to parental generation LC ₅₀concentration, is spread evenly across in 250mL Erlenmeyer flask, after acetone volatilizees completely, put into the citrus fruit fly adult that 50-60 head is sprouted wings latter 3-5 days, with hospital gauze sealing, be inverted 24hr, condition is: the photoperiod: 14hr illumination: 10hr is dark, temperature 26-30 DEG C, humidity: 60%-95%.After 24hr, the adult survived moves in dependent insect cage and uses artificial diet.Continuous process three generations.According to the LC of third generation adult from forth generation ₅₀the ensuing three generations of concentration process, by that analogy, makes its population obtain resistance, obtains the anti-Trichlorphon of citrus fruit fly and anti-effective cypermethrin adult strain.

(2) sub for detecting with each resistant strain in step (1) respectively, with sensitive strain adult for driven element, build each resistant strain poor specificity heterologous gene expression library respectively.

The poor specificity heterologous gene expression library built is after two-wheeled subtractive hybridization and 2 selective PCRs, and obtaining storage capacity is 9.48 × 10 ³citrus fruit fly adult anti-Trichlorphon strain differences gene expression library, storage capacity is 1.05 × 10 ⁴citrus fruit fly adult anti-effective cypermethrin strain differences gene expression library.

2, the preparation of initial gene chip

(1) the poor loss of weight group clone of difference random choose 2820 from anti-Trichlorphon and anti-effective cypermethrin strain SSH library, then shakes bacterium, extracts recombinant plasmid dna.

(2) adopt Nested PCR Primer 1F(sequence to see SEQ ID NO.1) and Nested PCR Primer 2R(sequence see SEQ ID NO.2) primer distinguishes each recombinant plasmid of pcr amplification.PCR system comprises 10 × PCR Buffer 10 μ L, 2.5mmol/L dNTP 8 μ L, F/2R primer (10 μm of ol/L) each 4 μ L, recombinant plasmid dna template 10-50ng, Taq archaeal dna polymerase (5U/ μ L) 0.5 μ L, adds sterilized water to 100 μ L.PCR reaction parameter: 94 DEG C of sex change 30S, 68 DEG C of annealing 30S, 72 DEG C extend 1.5min, 35 circulations, and 72 DEG C extend 5min.

(3) with alcohol-isopropanol method deposition and purification pcr amplification product, the unicity of its concentration of detected through gel electrophoresis and fragment, with citrus fruit fly G6PDH, 28S and β-actin gene for reference gene contrasts, product send Beijing Boao Biological Co., Ltd's point to make chip, and purified product adopts chip point sample instrument point sample on the carriers such as the slide glass of chemically modified, silicon chip, film or macromolecular material.Chip sample also comprises Hex as positive control, Blank(spotting solution) as negative control.Whole dot matrix is divided into 32 sub-arrays, and sub-array has 12 row, 11 row, and dot spacing is 150 μm, and the diameter of point is about 100 μm, and each sample repeats 3 times on chip.

3, the anti-Trichlorphon of citrus fruit fly adult and anti-effective cypermethrin resistance related gene fragment are determined in cDNA chip hybridization

Adopting the resistant strain total serum IgE used when building SSH library and sensitive strain total serum IgE for detecting sample respectively, carrying out cDNA chip hybridization, removing false positive differential gene; The sample selecting hybridization rear rise signal value on gene chip to be more than or equal to 2 is that Resistant Difference expresses fragment; Take recombinant plasmid as template, above-mentioned Nested PCR Primer 1F and Nested PCR Primer 2R primer carry out pcr amplification, described in amplification system and the same step of reaction parameter (2), purifying recovery is carried out to product and checks order, sequence adopts DNAstar software to carry out splicing assembling, remove and repeat and miscellaneous sequence, picking goes out single non-overlapping sequence sequence as resistance relative specific target fragment.

Chip hybridization: the fluorescent mark of probe rna sample adopts brilliant core cRNA amplification label test kit (Boao Biological Co., Ltd, catalog number: 360060-10), and concrete operations are carried out to specifications.The DNA of mark is dissolved in (3 × SSC, 0.2%SDS, 5 × Denhart ' s, 25% methane amide) in 80 μ l hybridization solutions, in 42 DEG C of hybridized overnight.After hybridization terminates, first in about the 42 DEG C liquid containing 0.2% SDS, 2 × SSC, wash 5 min, then in 0.2 × SSC, room temperature washes 5 min.Namely slide can be used for scanning after drying.

Chip scanning: chip LuxScan 10KA twin-channel laser scanner scans.

The collection of chip image and data analysis: adopt LuxScan 3.0 image analysis software (CapitalBio company) to analyze chip image (Fig. 1), picture signal is converted into numerary signal; Ensemble average value (global mean) according to cy5 and cy3 overall signal carries out the correction of sheet linearly to each chip; Lowess method is used to carry out normalization method in sheet; Each gene has more than three to repeat ratio, and namely analyze with SAM software, FDR controls within 5%, then with 2 times of standard screening difference expression genes.

4, the preparation of cDNA chip is detected

Be that template carries out pcr amplification to the recombinant plasmid of the Differential expression fragment chosen in 3, amplimer, system and reaction parameter are with step 2(2) described in, after product purification, fragment point makes cDNA chip, and method is with the preparation of above-mentioned initial gene chip.

cDNA chip prepared by embodiment 2 the present invention is to the drug-fast detection of citrus fruit fly resistant strain

1, the seed selection of citrus fruit fly resistant strain

Raise the sensitive strain of reservation as the parental generation F0 of resistance Screening of Strains Against with testing laboratory, raise under indoor identical conditions, adopt medicine embrane method, carry out by generation screening with Trichlorphon and effective cypermethrin respectively.Concrete steps are with aforementioned.Result obtains 3.21 times, anti-Trichlorphon, 18.23 times and 69.5 times of resistant strains respectively; Anti-effective cypermethrin 6.8 times, 16.2 times and 160.74 times of strains.Meanwhile, determining anti-effective cypermethrin 160.74 times of strains is 21.20 times to the cross resistance of Trichlorphon.

2, the extraction of citrus fruit fly resistant strain and sensitive strain sample total serum IgE

Each resistant strain and the sensitive strain emergence adult of latter 3 ~ 5 days of getting seed selection respectively extract total serum IgE (each 8 of male and female), and Total RNAs extraction adopts Trizol (Invitrogen) reagent method, the explanation of concrete operations reference reagent.Sample broke adopts granulated glass sphere polishing, and mill is MiniBeadbeater-16 grinding bead homogenizer (Biospec, the U.S.).

Total serum IgE quality examination: after RNA has extracted, gets concentration and purity that the micro-ultraviolet spectrophotometer of 2 μ L measures RNA sample, high-quality RNA sample OD _260/280should between 1.9 ~ 2.1.Get 5 μ L 1% agarose gel electrophoresis and detect its quality, citrus fruit fly RNA quality judging standard: the brightness of 28S band is more weak, and 18S, 5S band is brighter, meet most of total serum IgE in insect species and exist and hiddenly split phenomenon.

3, fluorescent mark and hybridization

The reverse transcription of each resistant strain and sensitive strain total serum IgE, fluorescent mark and hybridizing method are with aforementioned.Chip scanning and IMAQ and data analysis are all with aforementioned, and detected result is shown in Fig. 2.

4, cDNA chip detected result

(1) anti-Trichlorphon strain cDNA chip detected result

Anti-Trichlorphon 3.21 times of strains are relative to sensitive strain, it raises difference expression gene and has 12, anti-Trichlorphon 18.23 times of strains are relative to sensitive strain, it raises difference expression gene and has 50, anti-Trichlorphon 69.5 times of strains are relative to sensitive strain, and it raises difference expression gene and has 51 (as shown in Table 1 and Table 2).

Anti-effective cypermethrin 160.74 times of strains (be 21.20 times to the cross resistance of Trichlorphon) have 43 (as shown in Table 1 and Table 2) relative to the up-regulated expression gene of sensitive strain in anti-Trichlorphon different expression gene chip.

Table 1 citrus fruit fly adult anti-Trichlorphon difference up-regulated expression gene

Note: Tri-R ₁, Tri-R ₂and Tri-R ₃represent 3.21 times, 18.23 times, the anti-Trichlorphon of citrus fruit fly and 69.5 times of strains respectively; Alp-R ₃the anti-Trichlorphon of=citrus fruit fly 21.20 times of strains;--expression signal ratio is less than 2, cy3/cy5 and represents resistant strain hybridization signal value/sensitive strain hybridization signal value.

Table 2 citrus fruit fly adult anti-Trichlorphon difference up-regulated expression gene

(2) anti-effective cypermethrin strain cDNA chip detected result

Anti-effective cypermethrin 6.8 times of strains are relative to sensitive strain, it raises difference expression gene and has 4, anti-effective cypermethrin 16.2 times of strains are relative to sensitive strain, it raises difference expression gene and has 24, anti-effective cypermethrin 160.74 times of strains are relative to sensitive strain, and it raises difference expression gene and has 34 (as shown in Table 3 and Table 4).

Table 3 citrus fruit fly adult anti-effective cypermethrin difference up-regulated expression gene

Note: Alp-R ₁, Alp-R ₂and Alp-R ₃represent the anti-effective cypermethrin of citrus fruit fly 6.8 times, 16.2 times and 160.74 times of strains respectively,--expression signal ratio is less than 2, cy3/cy5 and represents resistant strain hybridization signal value/sensitive strain hybridization signal value.

Table 4 citrus fruit fly adult anti-effective cypermethrin difference up-regulated expression gene

Above detected result shows, the strain of different agricultural chemicals Different resistance levels can be distinguished and differentiate to the cDNA chip made by the present invention effectively, and this gene chip has the advantage of high-throughput, microminiaturization, integrated and parallelization.

CDNA chip of the present invention is made up of 118 anti-Trichlorphon relative specific target gene probe and 103 anti-effective cypermethrin relative specific target gene probes, can detect the resistance level of citrus fruit fly anti-Trichlorphon strain and anti-effective cypermethrin strain simultaneously.

SEQUENCE LISTING

<110> Agricultural University Of South China

The anti-Trichlorphon of <120> citrus fruit fly and effective cypermethrin Detection of insecticide resistance detect cDNA chip preparation method and

Application

<130>

<160> 225

<170> PatentIn version 3.3

<210> 1

<211> 22

<212> DNA

<213> Nested PCR Primer 1F

<400> 1

tcgagcggcc gcccgggcag gt 22

<210> 2

<211> 20

<212> DNA

<213> Nested PCR Primer 2R

<400> 2

agcgtggtcg cggccgaggt 20

<210> 3

<211> 18

<212> DNA

<213> G6PDH-F

<400> 3

gctgccgaaa cctacaaa 18

<210> 4

<211> 18

<212> DNA

<213> G6PDH-R

<400> 4

cgcttatcat agccaccc 18

<210> 5

<211> 558

<212> DNA

<213> S004(4)-A9

<400> 5

agtcattagt ggatacattt gtttgaactt tcctgatgtg aaaatcggcg ttattttcgt 60

tctcaaatcc ttccatagtg gattgtttac gaaaaataga ttattcgaac ccaagggatc 120

attgtgtgga tcacaggcgg ctgatcgatt ttcgaacttt gcgaattcct tgactagcac 180

agttttaata agttccggtt cacgtaacac taacgctggc ttgttgagta tgtaaatacc 240

aactgccgca gctgttgaca ttttagcatc cttataaaca tcagctagat ataggaagaa 300

actattggtc cttttaataa aaacttccgt attgccaatc agtggcgaca tatagggaat 360

attagttcgt ttccaataac caaaactgta gcgacaccaa acatagacaa gccccaagag 420

agccgccaag aggaatgtag tatttatcga cagcattatg cgcactgctt tcgaagcgct 480

taagaaaaaa tctagtatga aaggtattaa attctgtcga ccttttgtaa cgcgctttca 540

caataacact ccccatgt 558

<210> 6

<211> 696

<212> DNA

<213> S004(4)-B7

<400> 6

agataattgt gaaattactg tatggtcacg ttttgtagat cccaaagtag tgttcccaac 60

aggtgctaca ataggttctg ctattccaac gagagttgct gaagtgcaaa ttcgtggtga 120

agcaaaacaa gtagcaaaag aaggagtagt ctcctctacc ttgggaactg tagccgaagt 180

ccttaaaata ggacaaaagc taccagtagt tggtgaatac cttgcaatcc ctgaatggat 240

atgtgataaa ggtgcttcta tagctaaatt atttggttgg tctaagccga ctttagctat 300

ggacgtaaaa ttacggacca acaactgtat gagtaactat aatgggaaag attcgtctca 360

taaaatggct ttaagcgctg ataatgagat tgatagtcct ccaaacattg ctggtacgaa 420

agttgatgaa ttagcaattt catcgattgt gactatacca acgtattggc aaacctttag 480

ttggagtaac actgatcaaa cacaggatca aatcctgtgg attgataaag ttgctgcggc 540

gcgatttaat aagattgatg gaactacgaa cggttacgca actaccccta tggggttcct 600

tgctaatgtt ttcgctcaat ggcgtggttc tataaattat actttcaagt tagtgaagac 660

tgggtttcac tcaggtcgct tgagagtatt ctttgt 696

<210> 7

<211> 640

<212> DNA

<213> S004(4)-D6

<400> 7

aagataccgg cgaattgatc ctgaagtgca ccggccccag cgaagccgtg atgacgatgt 60

agagcttagc tctattaatg gttcagagag agattaagtt gaatacctct aaacagcaat 120

atgaaggaaa acatcaatga ataaaaccct cagtagatga gtaaaatatg aaggaaaaca 180

tcaatgaata aaaccctcag tagatgagta atcaaattga atgaaggaaa acatcaaaga 240

ataaaacccc atgattggta atcatcaata tgaaccatcc ctttcacggt aagggaacta 300

tgttgggctt agtttcagtt caacctatcc aataaccgaa ggaaaacgaa tttaacccgt 360

tgattacgta aaatcactat cgaaggaaaa cgttataacc cggaatgtcc gttaatatcg 420

aaggaaaacg aaatataacc tggttgctcc attaccgctt acactcgaag gaaaacgttg 480

atataacccg gttgccacgt tattagcact atagaaggaa aactttaacc cgatcgttac 540

aatcgtaatt atgtaacatt cgaggattct cctctgttga catatgtccg tctgatgaac 600

gaaaccatca catgaaggaa aaaaaaaaaa aaaaaaaaaa 640

<210> 8

<211> 840

<212> DNA

<213> S004(4)-H9

<400> 8

atatttcatc ttatgtatgt tctcataggt gaattcgttg ttgtgctctt tcaatgtcgc 60

attgatctcc agtcgcaacc tatcttgtat ttcctgattt ttagccaatt catacaatgc 120

gaagcccatg gtggttgaag aggtttcgaa gcctgctatg aggaatacaa aagcctgcgc 180

tgctatctcc tccactgaca gtccatcagt tgcgccttcc ttgtttttta attccagcaa 240

aatattcatg aaatcattac gttttacctg attcttctct ctatattcga gtgtattgcg 300

aactatgccg acatagaaat cttccacttc ttttgtagtc gagcacaaat tcaatttacg 360

cgctaaattt ggataactct caatgaatgt gtcgacaagt ttgccatgcc tgcgttcgat 420

cagcgccttg cgacccatgg tcacgaactc agcatcggga ttgtgcaagc tgttacactc 480

gagaccaaaa gcgcatgagc caattacatc ggcagagtag cgcgattgca gttcggtgat 540

ctccaaaaca ttgtcgggtg cgtttttagt tttctcacgc ataacttcaa ttaactcgtt 600

agccaatttc ataacagtgg gaaacattaa tttcattttg cccgaggtga atgtgggcga 660

cagcttttta cgcaacactt gccactgtga tctatccaac tgaaagagat tgccagtgag 720

cggatctgca gtttcattgt gaaaaatgcc acgatcttga aaattctgaa agtctttaat 780

aagtatcttt ttagttaatt ccaggtccag cacgaaaacg gctggtttta ggagaaagta 840

<210> 9

<211> 755

<212> DNA

<213> S004(5)-B10

<400> 9

gcacgtgaat acgacaaata tctctgcgca ttgaagatgg atgcgaaaac caactccaca 60

atactagaag taaaggaacc gaatgcgctc tacacaaccg atgtgatatc gatcacggcc 120

tacggcatac aagcaaatag tctcaacgat ccgaatggta ttttccgtaa aagtggcaag 180

aaaatcttca catttacttg gttgcgttcc atggagttca aggcattctt tttcataccc 240

atgatggtta agcttttcgg gttcaaagta ttttccaagc aggccacaat ctttctgcgc 300

gacacaatca atcatgtgat ggaggaacgc atcaaaaggg gtaatgtgcg caacgatcta 360

atcgacattt tgattaaatt caaacaggag gctgaagaag aactgaaaga aggtaagaaa 420

ccgttcattt tggagagaga cgctttggcg gcgcaagcgg ccattttctt ttcggctggc 480

ttcgaaacgt catcggccac aatgtcattc gctatgttcg aaacggcgca aaatccggaa 540

gtgcaacgac gtttgcgtga agaattacgc gaggcatata agagtaatga tggcaaaata 600

acctatgagt tgataatggg tctgaagtat atggataatg tagtgaagga ggtgttacgt 660

cattatccac cactaccatt cctcgatcgc gtatgcacac cgaaacctga tgaaaaaggt 720

tattccttgc aagcctttgg tttggacttt acagt 755

<210> 10

<211> 726

<212> DNA

<213> S004(5)-B5

<400> 10

taccatacac tcacatggct aaaattgcac aagcattacc tactatcaca aataatatgc 60

ttcagcagat aatatcttat tattcaggct tagggttgac agagagtgaa gctaggagta 120

atatcattct agcacactta ttttatctct cacctgcttt ccctgaaaaa taccgggtct 180

acaccggtgt taaagtgaca cggtttttac aacatgggac caatgatttt ccccaggcac 240

caactgtatc taatcaggtg cctttataca ccactagaga gggttttgaa tttggaatgc 300

tattggccta ccatggtctt gggaaaaact atacaagtga caatttcaac caagttgtaa 360

caaagcgagc tagcgcattc tatgcctcat tagggctgaa agctcctccc atcatagaac 420

ttccatttat taagtctatc tttgatgaac aatacttaag tcagtatcaa ggattcctgc 480

cagtagaatt gcggccttat ttaattgatg gattgaatgc ttcaaggaga attgcattaa 540

gaatgtatct ctctaatatc tcccaataca ccggaatgtc cctcatacgt gcatgccaca 600

gcatgttatt ccaacctgaa ctacttgctg gtttctttga tccaaacaca caatctgaaa 660

tcacaatatt tataaactgg tatacagaac attccagaca aacagatcag tttcctcttc 720

tctggt 726

<210> 11

<211> 348

<212> DNA

<213> S004(5)-C10

<400> 11

cagtaaattt aatatcataa cgttcaaaaa acgatgatat tgtagatgca ttaaattttt 60

gtaaaatatt ctccttaaca gtcataatta aatcatcacc atagaagaat acacatacat 120

tttctctaaa cgaagctaaa ctagtaatat ttgatattga atcttcacaa cattctctca 180

tcaaagttaa atatgctgta cgaatatata acatattaac tatgttattt aaaatagtcg 240

taataggaca tccagatggt gcaccacaaa ctacctcgta taccatattc aacattaaat 300

gttttgagaa tgcagtctct cgacccataa ttttccgtac ctcggccg 348

<210> 12

<211> 420

<212> DNA

<213> S004(5)-C7

<400> 12

gacgtcccgt ctccacggct gtagtcaagc ctttgcgcac agctaccgaa cttatggtgc 60

gcagatccgt tgccatgtcg agcgcggcaa acacacgtat cattgccgtt acgaaatcac 120

tgccataatc gccgaactct ccgctctgat aatcgttggg caacagataa tgatattgag 180

gccaatagct ctttgtaatg aagaacacac tattcgaatc ggaaggcttg aagttcttgt 240

ccaatcccca aatgaaatgt gccgaattaa taagccaaca aacattcaaa acgatgagat 300

aacgcagtga gaaggccaca aaggtggctg cggcaattga atcgccccaa tactcaagcg 360

gtgcattgat cggcaacaaa acgtgcagga agaagtagag tacctcggcc gcgaccacgc 420

<210> 13

<211> 834

<212> DNA

<213> S004(5)-D1

<400> 13

aattgtggtc ctgttgtcaa gaatgggtga gtgaaaaact tggaattgtt gaaccagggg 60

cgataactac atttgaacaa gatgttatga agtggcacaa agagattgag ttttggagcg 120

tgcttaaaaa taaggaagat gcaaaaaaag atccaaataa agcattgata atttcaaagt 180

tatatctcac aggaatgaat cataagcgtc aagccacttt attaaacatg gataggagaa 240

ttcatgatat tttgaacagt ggtattattg cggccagtaa gctatacaag tttgtggaag 300

aatccaacgc aataggtggt ggcacgcgct tcaaacctct gggtattgcc ttgtttggtg 360

aaagccaaat aggaaaaacc acaatggttg agaccttatc gcaagatttg ttgtatgaaa 420

tgggacatcg tgatgtagag acgtatcgta atgaaattta ctcacggcaa gcggaaacag 480

aatttttcga tggttacgtt aaccagtcaa taattatagt tgatgacgca tttgcaatta 540

gagatagtca aacaaaacca aatgctgaaa ttgcagaaac tattagaatg attaacgaat 600

tccctcatca cttacatatg gctattttgc aagataagaa tacatacaac acatcacgag 660

tcgtgctgtg gaccattaac aacattaata tccaaacacc ttcattatct taccctaaag 720

ctttccataa taggttgatg gaaaatgcct ataaagtaga accggctgat cacgttgtta 780

agatagtaaa agatggaaat gggcaagaac aaaagttatt ggatcaatca aaaa 834

<210> 14

<211> 278

<212> DNA

<213> S004(5)-D10

<400> 14

agcgaaatat aaaacgtcat ccatttagac catgttatgt agctgctatg gataaacgag 60

ctatagaaga aacatgcaat tggatttttg aaggtcatga agaacctgaa gcgtctatca 120

tagcttgtga atcaatgtta cttaatgcat ttggtcatgg tccagaatat tataatcatc 180

ttagaaataa agtagttgta ttctggcgtc aattaaatat ttatcctaga attcctttat 240

ggatagaagt agattggaga atctacgaag gtacctcg 278

<210> 15

<211> 351

<212> DNA

<213> S004(5)-D11

<400> 15

ttgagaacga aaataacgcc ggttttcaca acaggaaaga tcaaacaaat gtattcacta 60

atgactgaga tcggcacgga gttggaagcc catttgaatt cgtttgcgaa aacagacgac 120

gctttcgtaa ctgaagttaa agagctttgt gcaaacttca ccaccgatgt gattgccacc 180

accgcctttg gcattaaagc caatagtctt gtagatccga atgccgagtt tcgtgcgcta 240

ggccgaaaac agctaaccat tacattccgt cgcgctattc aattccttat cgcattcttc 300

tatccgaaat ggaccacaac attgcgtgtg aagattttgg tacctcggcc g 351

<210> 16

<211> 697

<212> DNA

<213> S004(5)-G1

<400> 16

gttaccagac aaagataccg gcgaattgat cctgaagtgc accggcccca gcgaagccgt 60

gatgacgatg tagagcttag ctctattaat ggttcagaga gagattaagt tgaatatctc 120

taaacagcaa tatgaaggaa aacatcaatg aataaaaccc tcagtagatg agtaaaatat 180

gaaggaaaac atcaatgaat aaaaccctca gtagatgagt aatcaaattg aatgaaggaa 240

aacatcaaag aataaaaccc catgattggt aatcatcaat atgaaccatc cctttcacgg 300

taagggaact atgttgggct tagtttcagt tcaacctatc caataaccga aggaaaacga 360

atttaacccg ttgattacgt aaaatcacta tcgaaggaaa acgttataac ccggaatgtc 420

cgttaatatc gaaggaaaac gaaatataac ctggttgctc cattaccgct tacactcgaa 480

ggaaaacgtt gatataaccc ggttgccacg ttattagcac tatagaagga aaactttaac 540

ccgatcgtta caatcgtaat tatgtaacat tcgaggattc tcctctgttg acatatgtcc 600

gtctgatgaa cgaaaccatc acatgaagga aaacattata accactgtcc ggttaaagac 660

agtaaatgaa ggaaaaaaaa aaaaaaaaaa aaaaaaa 697

<210> 17

<211> 582

<212> DNA

<213> S004(5)-H3

<400> 17

gcagagcgtt tgagtttcat aaatttaatt tctgacatat gaatgcgttt tgtcatatta 60

ttcgttctca tttctatagt tgtatctaat ggtaaacttt taattttatc aatataatga 120

tctatacata taaagctcca cccttttatt cctaaacaac ggcataatgt ttcagtattt 180

cgagaggtag ttgctactat aaagaaataa tttcttctta aggcaatatc tagagatttt 240

tgcatatcta gtgccatctc tggttgctta ggattaacat ttggcttctt cttgttagct 300

tctacatgtt gttgattata tgccatttgt ggtgatactt tacagaattg acaattacca 360

tttacacatt catcagtaat tacatgtttt ttaagcgcat ctgcatctat aatttttcta 420

ctatttaagt attctttcgt caatttcttt ttatcactgt aatccttata catattataa 480

cctttataca tacccgtaac ccctaatact atagctaata tccataataa tggtttagca 540

acacttttaa ttaaagatcc aattttcccc catgtacctc gg 582

<210> 18

<211> 533

<212> DNA

<213> S004(5)-H7

<400> 18

atatcgcaaa aaccagagta aacctgaatt taatccaata cattagtgat tatcttcaga 60

aacaaactac cttcagaaat gaaaaagtag ttatgtcgaa tctcacatct gacggagact 120

cagttcagtt cattagaact caaccgaccg cagaaactgt caataaaatg acaaagtgga 180

ccgacaaagt agtgcgaccc gtgtcaacga acgccgagtc aatcactaca ttcggagcga 240

gctatatgac tggatttcag ttatataaag aagcaacagg cggagatcat accaattggt 300

atcctctcaa agtcacagct tcaaatccaa ttcctgatga atggatagca aatcgcaacg 360

aaaatagaat cctaccacca ggattcaaca ttaatagatt taatgctatt agtgactcac 420

aagtcaatag gacaaacgca attgtgagac gtatgataac tagcttacgc taatcataca 480

aacaaaacat accgagaagg gggccacgcc gagtaggatc gagggtacct cgg 533

<210> 19

<211> 511

<212> DNA

<213> S004(6)-10

<400> 19

cgtaataact gtggcggagg ttgtatccaa accggccatc agcatgtcga tagccatcac 60

cacagccaca ttcttattta tgcgatagag tttctccagc acgctttgcg cttcgtcatt 120

ttgctcattt ttgaacttct ccatcgcctt ttcaataaag aatgtggtgg cgttggttac 180

atcatcataa gctttcatca gctctttaaa gccgggtgtt tcaacatact tccaaaatga 240

cggtgacaca tcgtatgtat tggccttatc gaaaaatata ttcataccgt cgacaatttt 300

taagacatta gcatctggtt tgtcgcgcgc taaaagaccc aagcgcgtgt tgagcgcgac 360

gactgcgacc gactcgaaag cccacatttt aatttcttcg gtaagatcgg tggtcaattt 420

gccggtgttg tggtccaact ttgagtccaa tctatccaaa aagtcttgcg caatattatc 480

aactgctggc aagttctgtc ggatattctg t 511

<210> 20

<211> 341

<212> DNA

<213> S004(6)-12

<400> 20

cgtacacatg tttgaacatt ttgcaaaatt gtggtcctgt tgtcaagaat gggtgagtga 60

aaaacttgga attgttgaac caggggcgat aactacgttt gaacaagatg ttatgaagtg 120

gcgcaaagag attgagtttt ggagcgtgct taaaaataag gaagatgcaa aaaaagatcc 180

aaataaagca ttgataattt caaagttata tctcacagga atgaatcata agcgtcaagc 240

cactttatta aacatggata ggagaattca tgatattttg aacagtggca ttattgcggc 300

cagtaagcta tacaagtttg tggaagaatc caacgcaata g 341

<210> 21

<211> 667

<212> DNA

<213> S004(6)-18

<400> 21

taagtaggac ttcatttttg gagttttcaa tgctcttcaa cgactatttc gcaaaatatg 60

ttttccttca tttactatct ttaaccggat agtggtttat cttgttttcc ttcaaataaa 120

tacatcattc tgacgggttt aaacttgttt tccttcatta actatcttca tagatagtgg 180

tttggcatgt tttccttcat agagttaata cagctagcat ggccgggtta taataacgtt 240

ttcctttgat tttagagata acggatctac cgggttataa caacgttttc cttcgagtgc 300

catgtggaga gagattcaat ccactacgat ataatactca tcatcaacaa tctcacactg 360

ttttatgcta tgaaacatgt caatggctga ctgtattaag gatccatcat accagagcaa 420

ggacgaacag agacatggct tcttccttgc aggcaatata gtccactgat gaacgtcatc 480

tacaccagta atctctattt caagggaggt ttcactgggc agcatagcgc cacaatggct 540

aggacgctct tcatgttcca atagagtcgc caaacagtca cattcgaaac gcaaaagttc 600

atatttgctt tcgcagttga tatcggcgag ttgcacaatt ttcattctta tgtgcaaagg 660

taacttg 667

<210> 22

<211> 507

<212> DNA

<213> S004(6)-21

<400> 22

aatggatata atgttacata aaaaatgttt taacattggt tgatgacaca ttatgtattc 60

ctgatattaa tggtgcaata gataaatata ccacgtgtga atggtattgg cagtatcaaa 120

cttcaggtaa gggtcggtgt ggtagcattt tgatatccga tatgaaccat aattcgccta 180

tcgttggtat gcatgttact ggaaaacctg gctcttctgg tcaagcggga tctgttggtt 240

atggcgaggt tatatgccaa gaaacgttgg aaaatgttct tgaacagtta gaaccaagta 300

tgattcttgt cgatatagaa acggaagggc aaatgtttga cggaaaatat atacatatag 360

atgttgataa accagaacgt gtaaaatcag cattagatgg taattttatc tttacgggtg 420

ttgttccaga aaaattttca cacaagcccc caactaaatc gaaattagtt catacgatgt 480

gctataatca gataacgaat tcaacgt 507

<210> 23

<211> 754

<212> DNA

<213> S004(7)-100

<400> 23

catggcgata taacgcctaa gttaattacc aatgaagaat taggttatat aatgccaagt 60

actatggccg tcatgtttgg gtataattcc gatatgaaac attttagtaa atgtgaaaaa 120

tttgtacaag aactgacgcc atttgataca cctattcaat taaatatgga tggacaagat 180

tatatgatgt tgcgttgtgg atattcgtat agcgtggcct ctgaaaaagg agattgtgga 240

gctgcgttaa tggttcttag taggtgtaat aatcgtaaat ggattggcat gcatgttgct 300

ggtagtagta ataatgaagg atattctata aaattgacac aagagttgat agctacacat 360

ctgaagcaat tagaaataaa cattaaacaa aaactaataa cagctgaggt gcataactta 420

tcaattgaag gtgcaatgtg ccccgatggc gactttatac ttaatggtgt aacggaaata 480

ccgctaagtg cgtctagcgt caccaaaata gaaccgtcac ttatatacgg agagttttcc 540

acaccgataa caaagcccgc ttatttaagg ccatttacga attatgaagg aattctagtg 600

gatcctgcac tgttagggct taaaaaagca ggtggcgaac aagttttatg tgacaaatac 660

atcttaaatg aagttacggc ttatatgaaa caacgattaa tgcaacaaca taatcaatca 720

attagtttgt caacgtacgc tagggtgatg acgt 754

<210> 24

<211> 547

<212> DNA

<213> S004(7)-109

<400> 24

ccgaaggtta agtgcgctga agtacaaggt tggatgagta tacctaatta tttaggtaaa 60

attacatcct ttctatctga acaactacca gcattaatgg atcgtttctt ctttacagtt 120

gaagagacac gcgaacaatt tcatgaagac gtagttacta ttaacaatac tacgttatct 180

cttcgtgata aattggctaa tcttaaatat gatttagtca agtgcttcgt tttagtgctc 240

accgttttcc ttctatgttt atatgaacag tataccttgt cgttgataac tgcgattcta 300

ggtgtaactg gtctcttttc cgatatacca agagacctta tggaatggtt gcgcgattac 360

ttttatcctt tacaagatga aagtaaagtg gaagttcaat tagatgccgg aagtgccatc 420

gctttatgtg ggccagccat attgagtata gttgcttttt atggtatcaa taagttaccc 480

aaggacagtg agatagaaat gttttcaaaa aaaatttttt ctattgacaa aggagtaact 540

ggtacct 547

<210> 25

<211> 429

<212> DNA

<213> S004(7)-138

<400> 25

tttgtcagta tgtcgactta tatgtgcctg gcggaagggt cgtacacgta aacctgaacg 60

aggattcatt agaaagttac gttgtatatc atcgaacatg atggtatttt tggcactata 120

ttgcggatat tggccccaaa ttagaccgag cggtttcaca tctacgacac cacgttcaat 180

cgtatatact gaaatcatgg cagtagagtc caagtagaac attattttgt aattgggatt 240

gttgctgaca cccagcaatt tcattttttc ctcaatccaa cgcataccag ttgccgacca 300

tataacaata tcataatttt cgtaactaga ttccagaaat tcatgtaaat atggtcgcat 360

tagctccgag gcattttcgg cgggagatcg atgatcgaat aaggtgtaat caatatcaag 420

tacctcggc 429

<210> 26

<211> 455

<212> DNA

<213> S004(7)-156

<400> 26

ctcccgctgt cgtggccgct gaaggcacct atgtggccaa gacccgtggc gctgtacgaa 60

agtattaatc ggtttataaa acttggtaac ggtcgacgcg tcagagcagt ttgtcgtgat 120

ttcgcaaagt gctggttgtt gtggccaatt attgtcgctt ctactaaaaa ccaattaccg 180

tggtttctac tgaaaattgt attacaatga gagttgaagt tctggcatgc ctgtgcctta 240

cgagtataat gctactcgcg gttgcacagg cctatataga gccacggaca gatttgggtg 300

aaattcaaaa tcgcattgta tcgggtagcc cggcatcact tggtcaattt ccttggcaag 360

taattttgaa aaaagatccc atcgaagatt tgttatgtgg cggttcaatt atcgctgcaa 420

attgggtgct caccgcagga cattgtacct cggcc 455

<210> 27

<211> 509

<212> DNA

<213> S004(7)-165

<400> 27

tctaactgtg tgaatcgcgc gttgaggaac tctgttttat tagcatgttt ctctactcga 60

cacttataaa gttccttgca gtgttcggca aattgttcgt aattcattga ttcgcctaca 120

ggataataca ccactccccc atcacgttca tgtctctcat atttcgtaat tttgtaaatg 180

gacaagtcaa taggaccatc agcagtattt atttttgatt gatccaataa cttttgttct 240

tgcccatttc catcttttac tatcttaaca acgtgatcag ccggttctac tttataggca 300

ttttccatca acctattatg gaaagcttta gggtaagata atgaaggtgt ttggatatta 360

atgttgttaa tggtccacag cacgactcgt gatgtgttgt atgtattctt atcttgcaaa 420

atagccatat gtaagtgatg agggaattcg ttaatcattc taagagtttc tgcaatttca 480

gcatttggtt ttgtttgact atctctaat 509

<210> 28

<211> 182

<212> DNA

<213> S004(7)-183

<400> 28

gtaagcagtg gtatcaacgc agagtaagca gtggtatcaa cgcagagtaa gcagcggtat 60

caacgcagag taagcagtgg tatcaacgca gagtaagcag tggtatcaac gcagagtaag 120

cagtggtatc aacgcagagt aagcagtggt atcaacgcag agaaagcagt ggtatcaacg 180

ca 182

<210> 29

<211> 762

<212> DNA

<213> S004(8)-197

<400> 29

atgctttact aatttgatcg actctattgt taatggaata gtgatgagat atctaatata 60

tcaacaaacg ggacaattac cactaagtga ttgtttctta ggtgatgact cagtctgttt 120

gactgaaaaa cccatcaacc tagatagcct atcggaattg gcctttaccg aattttcgat 180

gaagttcaat cctgataaaa gttatcaaac aggaaacgtg gagaacatgc actttctggg 240

atactataat ttattcggtc aaccgtataa acctatagat accgtaattg catcctcgtt 300

gtatcctgaa agaccagttc aagacaagtt tgaaacgatt actcgattta tcgggcaagc 360

gtattcatgc tttgaaccgg aagacgcaaa gagatttttc tcatgcgcaa gaatcttagc 420

tgaagaatgc aatcagctgg aggattctga aattgaaaat tttattcaaa ctcacccata 480

ccaatttaag tatttacata ctttaggtat aaaggtagag caaggggtaa aatttccaaa 540

atttaagcag tgggattttg tatcaattac attacctaga gcttgtaaaa agaaattttc 600

acgtcgccac ttcaatatag aagagttatc acaatatatt gaaaaatgga aaaccgacga 660

atagactaag acaaaactaa attaagcata aacttaaaac tagattaagc ataaacttaa 720

aatagaaatg aaatagaaaa aaaaaaaaaa aaaaaaaaaa aa 762

<210> 30

<211> 520

<212> DNA

<213> S004(8)-199

<400> 30

atgggcgttt ttaagcacat tggatttccg ataccgctta caacctttaa tccctgatta 60

aaatgttgaa agaacctgct gatcaacatc cgatcttcat ccaacgcaat gctggtgaaa 120

atgagacttg cttttcttat agagttaagc gaatatgttc agttttctgt cttttcgaaa 180

gtcaaccggg ctgttgccac ccattttgta agaccatatg cagtgaaaac tcaataacaa 240

atttacatag atatataata acgaaagatg tgttaaaagt tttctttatg gctattgtct 300

tatatctgat gcgtgattgc ctctttagac gtgacattct ttcattttgc cgtccgcgtt 360

ctggtgaagt taattacttg catggaaaat acttacggcg attatatatt cagaaaccgt 420

cgcttgatag tcgtttatcc caagagcagt ggcatgctcg ccttagaaga aattatggat 480

taacgattaa atgtctagcg tttgaaataa ctgccaaggt 520

<210> 31

<211> 819

<212> DNA

<213> S004(8)-217

<400> 31

tttagataat atagcatttc tcaaacgcaa gttcattttt gataatcata taaacaagtg 60

gttagctcct ctagatgaag cagttatatg ggaaatgtta aattggaagc gaactaatga 120

gatttcccca aaagatgctc ttagggtgag tatatcaaca gcagctagag aaatggctct 180

tcatggaaaa gacaaatatg atcagttttc tgctctttta caatccaatc caaatgttag 240

aaaatataga ttaaatatag ttatccctac atatgcctca atgttagcta caattgagga 300

tatggatttt ttttagatta gattatgaga gttgtgtgat cttgtgttat ttcaaaaata 360

ttgaagagtc taaagaaatt acacattgct acaactcaaa aaaggtgcac tatttagtgt 420

tacttccagg atgccttggt agcagcccta ctaaaatcca ggaaactctt ctaggtatgt 480

ttatgttaag tagcttaact taccgaacaa atttacttgc tgatataaaa acacaaaata 540

atactgataa tgataaggag tttatgcaga cacatcgtga acaagttata acttttcatg 600

atcaaggcaa tactacttat gatcaagcaa ttccagagtt tagtgacctg gatgaatcct 660

acttatcaat gactgtcgct caagacactc atcatgatat agtctccttc cttgaacgac 720

ctgttcgagt gtggagtggt aatatgacca ctagtaacac tgttggacag gtattatgga 780

ctgcttctta tcctagtgtg ttaatacaaa ataccacgt 819

<210> 32

<211> 515

<212> DNA

<213> S004(8)-225

<400> 32

cggcattatc agatagaaaa gaatacagca atttacactc tgatacttcc actcaaactg 60

aaacaaatca ttggtataga ttaccattgc acataaggat aggaattgtg cagctagccg 120

atatcaactg tgaaagcaaa tatgagcttt tacgtttcaa ttttgactgt ttggcatccc 180

tatggaacca tgatcaaatg gctaatcgtt gttgtggaat gttctatctc caagcaccaa 240

atgagataga agtcgctaat atgcacgacg ttcgtttaca ctctacacaa ccaggaagaa 300

aagaaccatg tttgtgttca tcattattct ggtatgatag cgcgctgatg caatcagcta 360

ttgatctgtt tcatagtatt aaacagtgtg aagttgtcga tgaggagtat tatattgtgt 420

tggattgata ttctctccac attgcactcg aaggaaaacg ttgttataac ccggttgctc 480

cgttatctca aaaaaaaaaa aaaaaaaaaa aaaaa 515

<210> 33

<211> 803

<212> DNA

<213> S004(8)-240

<400> 33

tatgatccgg ctgtcttaga aggagtaagc caccaaaaac tgtcaaattt gtattccctt 60

actttggctt ttgagagaaa acatggacgt atgatgaatt atataccagg cgtattaagg 120

cctgatagta taccagcaca agctcttcag cctcatccta aatacaagcg taatgtaact 180

gctgatccca ctagtcctga attcttacgc ctcctaaatg cacaagcagt catatctcca 240

gtgggaggcg gagctgaggc tatcctagct caattgctac aggccatgag gactaatgag 300

taattcaggg cattttgcat gggccctttc cctctatgat ttcattgact atatatataa 360

aaaaaccgtg agtaaagtca catactgcgt taaatcactg ttataatacc tgagggagtc 420

attttattgt caactggagt ctttaatata attaaatatc ataatggaaa atcttgaatt 480

tgatgagaaa ttacctgcaa gcacccaact gctttcgtat gatgcatact gtgactctat 540

gtatgaagtt gatataaagt cagttcagaa gcgtctaatg tgtctgttgt atgcaacaca 600

actaactcta gacaattcca cagcagagaa gaaaattgct agacatactg ccttaagtta 660

tatgagcatc ttaaagttat taaaagataa ctacctttcc tctgatgagc ctggcaatct 720

ggagcttgag atccaggagc gtttggttga tttaaaggtg aacatagcag agtatgaata 780

ttttccactc ccaggttttc tgt 803

<210> 34

<211> 830

<212> DNA

<213> S004(8)-248

<400> 34

tccctatctt taatagtaaa tcccaagtcc tataccgtga gagtaactta ttattttctt 60

caataatctt tttccgctct aaattcaatg cttcaccaag tgacatttca cgagcatcga 120

aaaactcttc tctataagct gcggcatcaa attgctttac tggaggcttt aaataacatg 180

ttctaaatgg tttttctcct aaactctcta agttcttaat taactcagga gaacctacat 240

gcacctcaat tttccgtggt tgttttacta actgtgtgaa ccgcgcgttg aggaactctg 300

ttttattagc atgtttctct actcgacact tataaagttc cttgcagtgt tcggcaaatt 360

gttcgtagtt cattgattcg cctacaggat aatacaccac tcccccatca cgttcatgtc 420

tctcatattt cgtaattttg taaatggaca agtcaatagg accatcagca gtatttattt 480

ttgattgatc caataacttt tgttcttgcc catttccatc ttttacccca tgtactctat 540

tgtgccacaa aacgtgtgcg taacgatacc ttcttgaatg tgctctttac acaaaccgaa 600

atcagttaat tttacatgtc cttgtgcgtc caagaggatg ttttctggct tcaaatcgcg 660

ataaataatg cccaatttgt gtaaatgtcc caaagctagt ataatttcac ttagataaaa 720

gcatgttgta tcctccaaaa atatgccttc acgctctaaa tgcataaata attcaccgcc 780

acttaaatat tccaaaatta aatataactt tccatcagtt tggaacgcgt 830

<210> 35

<211> 683

<212> DNA

<213> S004(9)-293

<400> 35

ttgcactcaa cccagccgac taccctacaa tatttcggaa tgagggtgta tggcccgatc 60

gacgaccact cgactctttt gcatatcatc gagaagtgca tcggccagat atttttcaag 120

gcatacacgg acttatgaca acatctggcg aagattgggc gaaaatacgg actgcgacta 180

acccggtttt aatgcaacct aggaatgcgc gactatatcc gaacacaata cttgagatca 240

atgacgaatt tctggaaaga atccgtcaca tacgcgatcc ggtaacaaac gagatgcccg 300

atgactttca cgacgatctc aatcgtctaa cgcttgaagg cattgtcggt gttgcgctaa 360

acacccgttt gggtatgata cataagaatc gagactctcc agaatgtaaa caactgttgc 420

aatcaatacg agactttttc gacctctccg aagatttaga agtgaaaccc tcaatttgga 480

aaattgtgaa aacaccgaaa ttctacaaat taatgaaaac tttggatttg ctcgcagatt 540

tgtgcaacaa atatattgat gaggcgctaa aacaaattga aatggatagg gatggaaaat 600

tcacctctga ggtgggtaag gaaaatagtg ttttggagaa gcttttgcgc atcgatcgtc 660

gcatcgccgt ggttatggct cta 683

<210> 36

<211> 372

<212> DNA

<213> S004(9)-308

<400> 36

gacaacacag cgtaacgctt ccacttaact ggacattaca cccaaagtct tcagaggtgt 60

agtcagatcc gtcctgtgga tcttcaaagg caacatatag ccgaatgaag tgatctgaaa 120

gaccaatcat tccataacag acgggtgtgc gtggtgttgg agtaaaacgt aaactcaacg 180

gactagttac aggtccaaat tttgcaaacg gcgaatcaca aatttcccga taagacatgt 240

acttatgtat tggtggatac acatctaggg gctgaaaaca cattccccac atgccacttt 300

tctttcctgt agggcacgta gataccaatg ttgctgatac cgaacggaca cagcatttag 360

tataattaga gt 372

<210> 37

<211> 694

<212> DNA

<213> S004(9)-328

<400> 37

gcaactaaca agttacgatt catgttacca tcgagtaaag cataatattc taattgatta 60

cacaaatcta aagttgtttc ttttcctaac atctttgtca acatattgtg agtaaacaca 120

ttaatgtctt ctgtatttat atccatgtta ggtgttttta cataatcaaa ttggaaaatc 180

gctatatttt caggtaaaag aattgtccat ctttgttctg ggaaatcttg aatggcttgt 240

ttaatagccg gcaagtaaaa atgtttcagt ggtgtggtgt cgattctttc caatcttatc 300

gtttcaatac atctttcttt tagcgataaa acaggaggaa caacacttgc gtcaacactt 360

tttcttacgt caaaattgtc cgtatacaaa atttcatttt cttccatatc ggggaaatat 420

ttgaaaaacg catccatgtc atcaacctgt attgctaact tttctccaag gtgctcagca 480

acaggcatac gagtagcaac aggcatagac ataacttcaa aagtttcctc aacacgggtc 540

tcaaagtcac gaccaatagt aacatcaatt attttcattt tgttattttt gttttaattt 600

ttattaaaaa gtaaatgtag attattttac tttaattatt gtttttccaa acagtttccg 660

tctcaatagg aaatttatag cgagaagaac atgt 694

<210> 38

<211> 221

<212> DNA

<213> S004(10)-394

<400> 38

ggctgcggtg gcatgcattt tgcctagttt tttaagtgtc aacaaggtct gcgcttcatc 60

catcccagct aaacgatcgg gagtaacaaa ttttaacact gcaaggtctt caaaaattat 120

agctgaatgc ttgtagtcca cataaatggt gtcggcaaat agtttatctg tgtcaccgat 180

ttcctttagc aactcagtca gttttggtaa aactttttcg t 221

<210> 39

<211> 609

<212> DNA

<213> S004(10)-401

<400> 39

acatggggcg tttttatgac acattggatt tccgataccg tcaatgaaaa ttctttcata 60

ataaaacctg ccgagttaaa tattattcaa cttttaaata tttatcactc aactgaaaca 120

aaaaaaagtt aaagccatgg aatgcacaaa agagatcaca tgtctacaga acttaactca 180

acaagttgct gagttatcaa aagcgttatc ccaaaatatg cagcatacgt tgtccctgga 240

gggaaaattt tcagctgcca tggatgaaat cgctaaactc caacggcaaa acgcgatgat 300

gctagatgtc atcaataaaa ccttatatag taatagaaaa gaagaagaag aagaggagga 360

agaagaagag gaagaagaag aagaagagga agaggaagag gaggaagaag aataaattaa 420

tttaactaca tttaataaaa tttaaaaaca aagaaaatag ttattattag cttattcgat 480

caaccaacaa aataaaacac aaaaaaaaaa atccaaaaac atatatataa cgagcgaata 540

acagaagaag aagaagacgc agtaaaaaga acatatttaa atgtagaaaa ctgctgaaat 600

tgtcaaagt 609

<210> 40

<211> 684

<212> DNA

<213> S004(10)-443

<400> 40

taaacaacgg cataatgttt cagtatttcg agaggtagtt gctactataa agaaataatt 60

tcttcttaag gcaatatcta gagattttgc atatctagtg ccatctctgg ttgcttagga 120

ttaacatttg gcttcttctt gttagcttct acatgttgtt gattatatgc catttgtggt 180

gatactttac agaattgaca attaccattt acacattcat cagtaattac atgtttttta 240

agcgcatctg catctataat ttttctacta tttaagtatt ctttcgtcaa tttcttttta 300

tcactgtaat ccttatacat attataacct ttatacatac ccgtaacccc taatactata 360

gctaatatcc ataataatgg tttagcaaca cttttaatta aagatccaat tttcccaagc 420

caagtagatg gttctatttt ggtttccatt attttcataa actcctctct attgcgttga 480

catttatcca gatatctttg actaaattca taagtatgct gaggtaattc atatttagta 540

ataaattctt caggtaatgg caattcagtt cctaattcag tatataaggt cactattttt 600

gcaacatatg ttctataatt ttcctggaat actttagtgt gaccacaaca ttctttatat 660

gaatcaatat gacaatgccc atgt 684

<210> 41

<211> 455

<212> DNA

<213> S004(10)-471

<400> 41

ataagatgaa gacagcccgc tcgttgttcc acgactgcct agcacaccca tacaattact 60

ctttcaacgc ctccacccgc aagtgtatgc catgtttcgc tgatagcagt atcgtctctt 120

ttttgtaagt cagttcagct ggtgtctgtg gtgcgaagga gaatctgaaa tgcttgatca 180

gcaaagcgag accgatcagc gcctgcattt tgccgaaacg caagccgatg caattgcgtg 240

gtccatcgcc gaatggtaac catgtacagg cgggtcgttt ctgaatttcc tcctcagtga 300

aacgttccgg atcgaatttc tctggttcgg gatagtattc gggatcgtaa tgtatgccat 360

agctgaatat taatacacgc atgttcttct cgatgcgata gcgtggatcg ccggtgtcat 420

actctgcctt tgcataacgc tccaaaaacg gcagt 455

<210> 42

<211> 241

<212> DNA

<213> S005(1)-A1

<400> 42

ttcatgtgtc tttctgtgcc catagcgcgt atggcgttgt taaagagccc gacacccaag 60

gcgattttca ccgacaaact cggacagtat gtgtgcaaag cctcgtttat gctcatcaaa 120

tatttggtct tttcaccgaa acttttctgt tcgatctctt tcggtatgag attcaaatgt 180

ggcatgcgat taacttgcat ggcgcaaaga tgcttctgtt cgtcggcaga gagtgtagat 240

t 241

<210> 43

<211> 619

<212> DNA

<213> S005(1)-B2

<400> 43

ctgctgttgt atagagagtg gaatgatttc gagttcggga acttctggtt tctccaagcc 60

agtgtatttg tttagcacaa attgctctaa aagtttatca acggtcatat cattaaattg 120

acgcttaaaa tccgaatgca cctgtccaac catgtcatga ttattttgca gcatctccaa 180

aaatgtggat aagaactctt tcatttgacg caaatggact tcttcaattt cttgaaaacg 240

cttgcacgtc tgtgtcatgc gccgctcgaa gtcattcttg atcggattat gcttatcgag 300

tagcgattta tattcatctt gtaatttacg taattttgct tcgacttttt caatgtcctt 360

ctgtgaggca ttgtccttgc gcaatttttc caattccagc actttgctcg cgtaaaggtc 420

acgtaacttt tgaacagcag ctgttgatgt ctgcatcgct tgcacagatt ccaaggtatg 480

tgattcttcc tccttaacgg ttttgtgttt tttatgcaat tcatcggcat acttatcgac 540

atccttcaca agctccgtga gcttttgcac catttgcaga tgtagtgtgg ataacttctc 600

ggctgaagtg cgtaatata 619

<210> 44

<211> 739

<212> DNA

<213> S005(1)-C1

<400> 44

tgttgcaagg taagacacca aatacgtctc caaattccgt gatatgcctt ccgtttccac 60

ggatcgtggt aagttactca ctaattgaag ggcggcttcg aaacacgcat ccgcctgtcc 120

taaacattga ttcaatagtg ccacttgacc ggtcagtaag tataggtcca tttgattttg 180

aattgctgta atactgggga ttgaaataaa acagaatgct atgcatgctt ttacaaacgc 240

tccagttttt tgagtataat ttcctttaac cacatgtcgt atcccgacgg ctaacttatt 300

taccgaattt accaatgtta tatagactgc gtccaaatca gtgaaagcct cacgtgcgtc 360

cacataaaaa ctaagctgcc gttcaaaatc ttttgggtaa gtaactttac gtatgaaact 420

acttatcaaa tgcgaaattt gtcggcgttc atcttctacg gattgagcgg ttacagagtt 480

actgagaatc tttccaataa acattatcgc atttatcaca atttcatccg agatattatc 540

ttcggatttt tggtggaacc ttatcagaat atttttacat acgactatcc tatttgaatc 600

tttcttaaaa agatctaaat atggtaaaaa attttcctga atgagcaaca actccgtatt 660

ttcaaaatgc tgcaaaattt gatctaagat attttgcaac tgagtataaa atctttcata 720

tgttcggctt gcgttcact 739

<210> 45

<211> 709

<212> DNA

<213> S005(1)-D12

<400> 45

tgactattcg gcatgttgac atgttgaacg cgttaacgct gtaacggtat atagacagat 60

ttttatttga agcagcagca gcaagttgtt gtagcagagt ttgttgtgaa aaatattgat 120

atataacaaa gaaaatttta attgataata tttcataaat aaccgaggtg ttcaaccgaa 180

gtgaaacttg ttttattaaa aatatgaaaa taaataaaac gaacagtgtg tgttgaaagt 240

attgttgtgc gatcataaac acgtgttgga taattacagt gctctatttt gagcacatat 300

aaaataattg atgcatgctt ttgggagcat agatgtgtgt gtgttaaact gaaaggaatt 360

agaaactaaa cttcctgaaa ttattgttgc aagtattcgt tgaaatcaag cgggtttgta 420

gcaaacaatt cactcagcaa tcaaacttgt atttgatttc cgaaattacg cgcctattta 480

taactagcat acattcaggc gcaggtgtgt ttagcttcat taacaagcat atctaacggt 540

taacggcttt tatttgacaa ttaatataac tacttttcgc ttagtgaagc ttttggctta 600

tttccatcat ttgttcttac tcactcattt cgtttgcctg agtgtaaacg cgaaaacgcc 660

cacgagctta agcgtggcag ctaataccgg attaaacata gtgtagaag 709

<210> 46

<211> 772

<212> DNA

<213> S005(1)-G8

<400> 46

aatagttcgt gggcggggcg agctgatcac ggttcaatgc accattcaaa tataaagtta 60

ctggacttgt cacacaacaa catatctctg atacatccag gctacttccg gccagcggag 120

atttcattga cccacctata catgggctat aactcattga tgaatgcaac gcgagatgtg 180

ttcggtaata tgccgcatct gcaatggctg gacctgtctt ataatagtat acgcgagctg 240

gatttcgatg cgtttaaaaa taccaagcag ctgcagttaa tatatctgga tcacaattac 300

ttaacggaca taccccagga catcttcaag cccatctacg ccttgcgcgt tatcgatttg 360

tcacataacc atttacgagg tttgcctgac aatcttttct ataacggcgg catggaaaaa 420

atggacgttt cgcacaatat gctgctcaaa ataccctcat catccctgtc cagcttggcc 480

gctttaacgc tttgtgagct gcacttatcc aataacttta tttcaactat acacagcatg 540

gacttatcca ataagttcag gtctctgcgc ttcctggaca tttcttacaa ttacttgctg 600

cggattgacg acgcggtctt cgccacaatg ccacgactcg ctgcgcttga cctctcacac 660

aaccgtgatc tcaaagtgat ggacaagtcg tttatgggtc tggaaaattc actcataaaa 720

ctaggaatgg agaatgtttc tttgagcact attcccgaga tacgcttaaa gt 772

<210> 47

<211> 717

<212> DNA

<213> S005(2)-A8

<400> 47

gcacctgtca aaggtccgga aaatccaaat ttaacacgac aaatgcgagc gccgcgtaac 60

actctttctg gatatgtaga aaaggcgcac attaatgctt ttgaatttga aaatcagcga 120

cgcacattcc acacttacgg atatgcgttg gatccctccg tcgacgaagt tgcagacggt 180

caagcatttg tcggtgattt acaatcggca tatgatgatg atggaaaaac tgtgtttgaa 240

tcaccgaaac cgaaaaggaa acgtaaacaa gataaaaacg acaatccaga agacattgaa 300

ggtttcgttg gtccttgggg taaatataaa aatgaacaat ccatagcacg gccaaacgac 360

caagaaaagg cagaactgga agagatcctt gctaaaaaac ataggcgtaa ccgcattgtt 420

gaggataagc cattggagga aaaatctgtt cttcacatca aagacgccta cgattatcaa 480

ggacgttctt atctacatgc tccacatgac ttgggtatca acttgcgttc aacgtccgct 540

cctaacaaat gcttcttacc aaaatcacat atacacactt ggactggcca tacgaaaggt 600

atttcgtcaa ttcgatggtt ccctcaaaca gcgcacttac tgctttcggg ctcaatggat 660

tgtcgtgtga agctgtggga agtgttcggc gagcgacgtt gcattcgtac attctct 717

<210> 48

<211> 173

<212> DNA

<213> S005(2)-C7

<400> 48

attatgtatt cctgatatta atggtgcaat agataaatat accacgtgtg aatggtattg 60

gcagtatcaa acttcaggta agggtcggtg tggtagcatt ttgatatccg atatgaacca 120

taattcgcct atcgttggta tgcatgttgc tggaaaacct ggctcttctg gtc 173

<210> 49

<211> 561

<212> DNA

<213> S005(2)-E8

<400> 49

gaggaatgtc gcaagcgaaa tcttcgaacg aaggcaacga ataagtaaaa taacacattc 60

cacttcccca gctccaagaa ttaaccagac attcacgcat acaacaaatc caatgacgac 120

aacctgcgca caataccaac aaccacaatg aaacaaattg gacaatgatg tcatcagccg 180

ttgaaatctc agtccgagca gcttgcgtcg caaccaaatc actgaagcaa aactttcgct 240

ctcatcaatg ctgaagctat gtagcagcgt cgaactttgg tcaatttggt atacagcggc 300

aacagcagca acaatactac taagtcttag tataagtata agcatacata catacatata 360

gttattgcga actgagttgc tcacagttct cttcgatttt aatggacgtt ctgcactgga 420

gggcaaagtc ttcgcccaaa gattgttata atttccaaaa gaggaaaaga gtgcagtggc 480

ttctcagatt cgtcaaaatc attccaccgc acgctgtgtc taacacggct ccacatggct 540

acgaacgcca ttaaatcaca a 561

<210> 50

<211> 659

<212> DNA

<213> S005(2)-G1

<400> 50

attccgttga taccattaca tcaaaatatg gttgatacca aaagtcatag ccacataatg 60

ccacttggtt gcccttggtc cacgtgccga cacaattgaa attattcgtt tcgaagagaa 120

taaaatcgcc cttggcatta ccctcagcat cgcccagcgt tgaaatcatt acattaccat 180

cagccagaca atgcgttgta tgcggcgcag ttacgttatg ttgcttgaat acatcaccat 240

ctatggtctt gtataaagag ggcttgcgtg gatcagtcgc cacatccatt acgtaaatga 300

aatcggaaat aatcgaaggc aaaataagtt tgtcacgttt cggtatttgt ttagaattct 360

catccacata gtagcagctg gagcaagcat tccagcccga gtgatggagt tcattgcctt 420

tgcgattggt aaaggttcta tgaattacct gacagtaagt cggactttcg ggatccacat 480

ccacagttga taagtaatca ccatggggtt cgtctagatt aggctgcaca gtaacggtgt 540

agagcaattt ttcgcgagga ccatttttca tggcgtcaag tgggctctta tagccgggac 600

catggcaaca agctttgctc gacattttta gggtttttcg gttgttaaca atttcttat 659

<210> 51

<211> 560

<212> DNA

<213> S005(3)-A5

<400> 51

atttataaat aagagcggta gcagaacatt ttgcagaaaa gctagtttgc cagccgtttt 60

cgaccaacca taaattcata gaggtgcata cggaaagttg gacgcagtcg acttccaact 120

atctacaaga tgaatcggcg attttctcga gtagagtccg gtggtgactt aaaagatttc 180

cttgatcgcg gtgacattgc ttctcgagaa aatcgatgga atttcgaaat agcatgggaa 240

gtggctaaca aggttggagg catctacaca gtcattcgtt ctaaagcata tgtttcaaca 300

gaagagatgg gtgagcagcc gtgtctcatg ggcccttata aagagcattg tgcacgcaca 360

gaaatggagg agatagaatt tccacgcggt aatccacttc tagatgcagt gaatacaatg 420

agaacgcgcg gttataaaat acatacagga cgttggttag tcgatggtaa ccctcaactc 480

atactgtttg atatcggctc aggtgcatgg aaattggatc aattcagatc agagttatgg 540

gaaaaatgcc atgttggtgt 560

<210> 52

<211> 491

<212> DNA

<213> S005(3)-A9

<400> 52

gcaatattat ttttttggct tttccttaac taattttgtt tattgtcttc ttctttgggc 60

attgtaaaat tgcattaatt ggaattgtaa cgcatacgcc atggtgttct ggacgttagt 120

gatcggtgcg atcataatcg cttggctcta caaactaaat aaggattatg tggtgctgac 180

attatttact aagcgagtga caactgttga tggcacaccc ttggagaatt cagtggcgct 240

gcccaaagga ctcacaatat ttggcaatgc cttcgatttg gctgtatccg gcgatgacat 300

cttcgacttc tcgcgcaaac tagctgccga aatgaaacgc agttatctac aatatggact 360

attcacacct ttttaccaca taatcaccgc tgaggatgct gaactagtga tgaacgatca 420

aaacaatcta accactaagg gtattattta taattttttg gagccattcc taaatagagg 480

actgctgacg t 491

<210> 53

<211> 700

<212> DNA

<213> S005(3)-A11

<400> 53

gtggtgagca gtggtggcag catttacata gtgtcaacgc tcgtaaattg cgtgtcacgc 60

ttaaaataat aaagtgaaaa tcgcgcacaa aaactcataa atacttccaa tatagtgtcg 120

aggacaagtc gctaatactc aagtcacgcg ctttttgtaa ataaccgaat aattgccgaa 180

gtttttgaca caaatcaaag ttgcgttggc gcgaagtgtt gaccaacaaa gtgcggtttg 240

cgagcaccag agcaaactaa gtttcgtgaa gttgaataaa ctattaacgg tttatgtgag 300

tgatagtgac gtgagtgcat tagatccgtg attcataaca atgttttcgc ttgacaagaa 360

cattcatcaa tgtcgccacg gtcgggaacg aaacgcgctt ttgacttaca gcatcctgtt 420

atgcgcgttg gtggcgctaa tgctttgtct gcctgtgggt ggggacgatt tgagagcata 480

taagtatgtg tccagagcac gcgaaaggca gctggaaaaa ctcgcgatac gcataaatga 540

gtccgttaac ccaaatcggt atccctgtga gagcttctac gattatgttt gcagttatag 600

taaaccgcta ttcacgatac ttggtcattt gcccgaactc gatgatctca tgaagttatt 660

tactgagctg cagcaagatc ccgagaagtt tagtgccaaa 700

<210> 54

<211> 428

<212> DNA

<213> S005(3)-C11

<400> 54

ttgtcttagc ctgtgctatt cgtattgttt cctttgtgcc ctcttcgttc caaaatgttt 60

gcaacgccga agtggatcca aaaagttgtg catcttcatc gctaagggga ttttgttcgt 120

tgactgtgtc aaaattattt aaatttatct tgccactttg ggaaacggta tcatcgaaaa 180

taattttgga tttgtcgtaa ctaattttat caacagaggt tcgccgcgaa cgacgtcgca 240

gtagttgctc aagcatttcg tttattaaac tagaatcagc tttgactgaa cttaatctat 300

ccaaacattc ccgcggtatt gccgtagtat ctcgtggaag gaaagaggtt cgcgcatctt 360

tcataatatc tctatcttcc acttctatag tttcactaag accaaattta aatgatatac 420

atcgttta 428

<210> 55

<211> 836

<212> DNA

<213> S005(3)-D12

<400> 55

gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 60

tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 120

tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 180

cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg 240

catccaacgc gttgggagct ctcccatatg gtcgacctgc aggcggccgc gaattcacta 300

gtgatttcga gcggccgccc aggcaggtac aatggatata atgttacata aaaatgtttt 360

aacattggtt gatgacacat tatgtattcc tgatattaat ggtgcaatag ataaatatac 420

cacgtgtgaa tggtattggc agtatcaaac ttcaggtaag ggtcggtgtg gtagcatttt 480

gatatccgat atgaaccata attcgcctat cgttggtatg catgttgctg gaaaacctgg 540

ctcttctggt caagcgggat ctgttggtta tggcgaggtt atatgccaag aaacgttgga 600

aaatgttctt gaacagttag aaccaagtat gattcttgtc gatatagaaa cggaagggca 660

aatgtttgac ggaaaatata tacatataga tgttgataaa ccagaacgtg taaaatcagc 720

attagatggt aattttatct ttacgggtgt tgttccagaa aaattttcac acaagccccc 780

aactaaatcg aaattagttc atacgatgtg ctataatcag ataacgaatt caacgt 836

<210> 56

<211> 519

<212> DNA

<213> S005(3)-D8

<400> 56

taccatactc agatatggta gattattgat agtctcatat gacattttac cccgttcagc 60

tacaagagct tcacaaatct cattgcgcag acgttcttgt aaatcgggcc gtttcgataa 120

ttcgtataga gcaaatgtca tcgtcgatga tgacgtctca aaaccagctg acagaaatac 180

ggcagcttgt gctgtcaaca tatcctgtaa ctgaggacta tactcgcctt tcgcaactgc 240

ctcttgttta aggctcacca atacatcgat cagatcgtta cgcgttgctt tgctctcctc 300

tcttgacgtc ataacatgtt cgatggtgcc gcgtaaaaag gaactgaact ctgttgtaaa 360

aagtttaata cggaatgtag agacccactt ggggacgaaa aaggcgatga agaagtcaat 420

ggcacgacgg agtgtaaagg tgaacaattt tcgtccttga gtgcgaaatt cgccgtttgg 480

atttacaaga ctgttggctt ttacaccaaa agcgatggt 519

<210> 57

<211> 639

<212> DNA

<213> S005(3)-H12

<400> 57

ggcacaaact aagacttctt ttataaataa tatttttctt gagtgcgcat aatgctgtcg 60

gtaaatactg cttgcctctt ggcggctctc ttggggttgg tctatgtttg gtgtcgctac 120

acatatggct actggaaacg caataagatt ccctatatga cgccgctgcc actaattggc 180

aatatggaag ttttgtttaa aatgagcaat agtttttatc tgtatctatc tgacgtctat 240

aaggatgcta aaatgtcaaa ggctgcagca gtcggcatct atatactcaa caagccagcg 300

ttagtgttac gtgaaccgga acttataaaa tctgtgctca tcaaggaatt ctcaaaattc 360

gccaatcggt cagccacctg tgatccacat aatgatgctt tgggttggaa taatctattt 420

ttcatacgaa atccacaatg gaaggatttg agatcgaaaa taacgccggt tttcacaacg 480

ggaaagatca aacaaatgta tccactaatg actgagatcg gcacggagtt ggaagcccat 540

ttgaattcgt atgcgaagac taacgacgct ttcgtaactg aagttaaaga ggtttgtgcc 600

agcttcacca ccgacatgat tgccaccatc gcttttggt 639

<210> 58

<211> 366

<212> DNA

<213> S005(4)-A2

<400> 58

aaccatgttc atagagtgag caatcagcca ctaaactcaa aaattcaaac aacaaaagaa 60

atatttcatt cttcgatcga aaaacaaagg ccaaagccac tcgtttgaaa aagccgaaat 120

ccgttaatgt atgttgaaca gctgggactc cgaggagatc acagtcgtta cagcatgcac 180

acgaatttcc actcgaacaa aacaagggag tagaccaatg caaattttga ttgaagaggg 240

aaactcgaat attgtctgaa tttccaagcg aggaaaactg ctcccaatat tgttgaagca 300

tagctgatca gatgcgtgac tcggttctat ggttccgaag aaccgacttt gattcaaaat 360

tcttgg 366

<210> 59

<211> 564

<212> DNA

<213> S005(4)-A3

<400> 59

acggatttgt taactacaaa ctcattaatt aaaactttct gaattctata gtaattcctc 60

gacaccttct taatgacctt ttgtgaccga gagataaatt gtctaaatca aatagtcgcc 120

agtctcgacc gaattgaacg cggaaataac ttaccatctt taactttcat ttatttcata 180

ttaattgtta tctgcgtatt attgacacca tgggcaaaac tcgtcaatcg tttagaaaac 240

gaaattcgtc gaagaagagg aggacaattc gtagacgtcg aacttcagga cgcgcgtcaa 300

ccgttgttaa atcaaccccg gctgagtatc tcatcaatgg agcaaggact ttaattggtt 360

tgttacctgg tcaggcaata ttaaaaccaa tatctgactt catcttcggt aaaacatctg 420

tgtcatctct tagctatgca gagctaatta gtggcccaac tccggcaaaa actcactgca 480

ttggacttca agtttgtcta tctttgactc cagcaaactt ggttatcgat tcacctgaaa 540

atgctattca taatggtacc tcgg 564

<210> 60

<211> 757

<212> DNA

<213> S005(4)-A8

<400> 60

ccggaatcga cccaccctag tgtgtatgcc ataggtttgc gcttaaagcg ctttatacac 60

tgctgcggag tggcctcaca gaggatccgg agtcaacaag ttcacctact caatatttcc 120

tgattttaat agatatacct gctgcctcag cattttttcg caccttaagt gggcgttata 180

ttccgtggta gccgttttct aggcacactg ggtgtagctc ataaaaactg acattccgcg 240

ttgacacttg ttaaatactt tttttacggt tgttgaagaa aactttttga aatcactgca 300

caaattctct taagaaaccg aatccgatgg catgcgcctt ttctcttgcc gacaaaacaa 360

aactactact ggaaacacta tcgtaattcc atcagatcgc tttctcggaa caccatcgta 420

tagaccatac ataccaaatg tgctggaaga tttcgagagt gaatgaaaaa atattttctg 480

atttctggta gtataaaaga gtataaaaga tcttaatttt gatttcgtca ataaaaagga 540

ttataccagg gcatgaattt atctttcagt ttctatggaa gctggatgct atagtggtcc 600

gatatcgatg gttccgaaaa atgagtagca tttagacgga caaactgaca tgactacatt 660

gagacagctc gacgaaccga tcattgaaga atgttacaac aaaacaataa agtaaaaaat 720

tagttgaact tgtggcgaaa actccgtgaa cgaggaa 757

<210> 61

<211> 390

<212> DNA

<213> S005(4)-B8

<400> 61

aagaatttga ttctttctac caactggttg tcaaagaaaa gggacatgtt tacgtttgtg 60

gtgatgttac aatggctgaa gatgtctatc agacaattag gaactgcatc gcaataaaag 120

aacaaaaaac tgaagcggaa gtagaagcgt tcttgctgac attgagacat gaaaaccgtt 180

atcacgagga catattcggt ataacatcgc acgctggcga ggcgcgcaat aaatcaacac 240

ttcgacgggg ttcaagaaca ctaaatgcgt tatagagcaa ttctcgatct gcagaacaaa 300

ccattgggaa agagagatag tgccggccac gactgctctt tatgattacc acaaatttgt 360

taaatatttc ctttttcaac ttatacaaca 390

<210> 62

<211> 839

<212> DNA

<213> S005(4)-G1

<400> 62

atgggaaata tagcccgatt attgttgcac tcactatgaa tgaaggtcat cctaaatttg 60

atagtgtatt tgctagtaat gatgcttggc agaatagacg agatgttttg attaaagttc 120

atttaaaacc acattgtttt ggaaaagatg cagttaaagt tcaggaactt ccaaaagatt 180

ttgttaaagg tcataaccat cttacattta ctgttaatag atatgttaat tcaggcactg 240

ataatgatac ttctcttatg aaagaaattg aagttccacg agaaaatgat aaaggtgaat 300

ttgtaaaaca taatggtcat tttatcatgg aaaagaaggt gcatgtttct ttaaaacaaa 360

ttaaatggta tttggctgaa atttttaaag agaaatatgc taatatgcgt caagaatatc 420

ttaaagattt agctttagca aagagttttt atccaaaaga aagtgagact tttgaagtta 480

atattaaaga ttatgtaaaa tttgttaaag ctaataggcg acatatgaat gaacaagaga 540

agcataccat ttatatagca gagaagttat caggatcagt aaagtgtgat aaatgtaacg 600

aatctgctaa ttgtatgtgt gatttaaatg tatttgatgt taattatgtc agtggcaacg 660

ttcaaccgga agtaaacgat agagatgaaa taggtccagt tgaaatgatg cgagccattt 720

atcaacgagg cgaattaaat actgctactt attttgatga attggatgca ttagaaaaat 780

tatttgatga aaatttccat agtgatctaa gagaatttct acctcctcaa tctttagac 839

<210> 63

<211> 266

<212> DNA

<213> S005(4)-G2

<400> 63

gaaaccctca agacattccg ggctgaagtt gtggaacata ctcaagggcg tcttccaaat 60

gtccctgttg tcataacacc accacaagta actcagagtt tcggtgttac ccagcaatca 120

cagcctcaag tccagaagaa gacgatgacc ctaaatgaac ttttagctag gaaacgaaat 180

tagatgtgat caagatagac atgacttggt cctttaatat gtttggtcag tcttttgaag 240

aaaaaaaaaa aaaaaaaaaa aaaaaa 266

<210> 64

<211> 771

<212> DNA

<213> S005(5)-A5

<400> 64

aaatcttcga acgaaggcaa tgaataagta aaaaacacat tccacttcat cagctccaag 60

aattaaccag acattcacgc atacaacaaa tccaatgacg acaacctgcg cacaatacca 120

acaaccacaa tgaaacaaat tggacaatga tgtcatcagc cggtgaagtc tcagtccgag 180

cagcttgcgt cgcaaccaaa tcactgaagc aaaactttcg ctctcatcaa cgctgaagct 240

atgcagcagc gtcgaacttt ggtcaatttg gtatacagcg gcaacagcag caacaatact 300

actaagtctt agtataagta taagcataca tacatacata tagttattgc gaactgagtt 360

gctcacagtt ctcttcgatt ctagtggacg ttctacattg gagggcaaag tctttgccca 420

aagattgtta taatttccaa aagaggaaaa gagggcagtg gcttctcaga ttcgtcaaaa 480

tcattccacc gcacgctgtg tctaacacgg ctccacatgg ctacgaacgc cattaaatca 540

caagtagtga aaccaagaat tttgaatcaa agtcggttct tcggaaccat agaaccgagt 600

cacgcatctg atcaactatg cttcaacaat attgggagca gttttcctcg cttggaaatt 660

cagacaatat tcgagtttcc ttcttcaatc gaaatttgca ttggtctatt cccttgtttt 720

gttcgagtgg aaatttgtgt gcatgctgta acgactgtga tctcctcgga g 771

<210> 65

<211> 771

<212> DNA

<213> S005(5)-B7

<400> 65

atggagttcg atacgtctga aggctttcct tttgtaagca ttcgtcccaa aactgcaaaa 60

gataagcggt ggttatttga tttatatgaa acttctatgg gttatggttt gagatcaata 120

gataagcgtt tagttgaagt tatggatatt aaacagcgac aaagagaaaa aggtattgtt 180

ccgcttacta tttttacaga ttgtttgaaa gatgctaaat tacccgctga gaagtgtcat 240

aaaactagaa ttttctctat atcaccagtt gattttacta ttcaatttcg ccaatatttt 300

tatgatttca ccattgcttt tcaagaagct agattctcta ttgaatctgc tgttggaatt 360

aatgtggatt cttatgaatg gaatgaaatg gttcgtatat tgttgagtaa ttcaactaaa 420

tttgtttgcg gtgattattc taaatttggt ccacgtttaa tgagttctgt agtagttaga 480

ttctttgaga taatatgcga ttggtatgaa gcttatggag attctgatct aacaaatagg 540

cgcgttagaa tggctatggc acatgaaata gcttttgccc aacatcttat gcttaatatg 600

gtttatcagg ttctttgtgg tgcaccatca ggttgtccca ctacaaccat tcttaataat 660

ggggtaaata tgatgtatct ccgttcagca tatttacaat tatcaaggga aaacaatatt 720

gggttgacat tagaatcata taaaaataat gtttgttcaa tattttacgg a 771

<210> 66

<211> 709

<212> DNA

<213> S005(5)-E11

<400> 66

tcaagtgaac ggtttatgta gaaatttgag tgagcattcg tttaaccgat ttaataaaga 60

aattgcataa gttctcatat ttttcaatac tttaaccgtt atggatggaa aaccaaagaa 120

aacgcgccat cggaatcggc caagaaataa agcaaatcgt ttaaaggagg acaacggcca 180

gacctcgaat agtgaattaa ataacactga gggcgtagcg ttaaatattg tgggtcaaca 240

aaaagcactt gttaacgaat gtgtaaatga gaattacgga gctctaacaa aaaccacgcc 300

gagtggtagt gaattgccaa cgcaatcacc gcaagattcg gacaatggtt tgagattact 360

gcaacaaatg atgcgcacaa agcttttaca gcaaggcaaa aagagtgaaa aaggcaatgt 420

tgatacaaaa gccactgaaa aggtcgataa aaagggtcaa aaaagtagtg ctagtttgag 480

caaaggcaaa gaaagtggta aaacgacaaa tggaaaagcg gcagcggaca atttacaaaa 540

gcaaagtgca tttgttgcaa gtttgcagag aaaattagag agctcagtgg caaacggcag 600

caccgattta cctaatactt tattggaaaa cctacaaaaa ttactccatg agggaccaat 660

aagtgatgta gagtcctcag acgacgagga ggattacatc gagtatatt 709

<210> 67

<211> 706

<212> DNA

<213> S005(5)-G6

<400> 67

atgggacaat tcacgggaaa gtagagagtg atggaatatc tagagacaca attagagatg 60

acaatatacc tggcgaagca gctgcacaat ttgaagaaca ctctacaaaa gttggcgaac 120

gcaaggtata tgaagacaag gtgaaagcag taggtcctaa gaaatcaaat acactgagca 180

gtgcttatag aaaacgacgt gatcccagac gtatgacgtt aacacgctct gcaactatcg 240

aaattgagga gcgctttcag gcactggaac gcagaatgag tgaagatagt tttcgaacag 300

acaaaatgaa tactaacggt gagaatatgt ttgttgaaga tagcgcaaga aaaattccaa 360

catcagctga gttggacaaa aggttgaaca cactggagaa tgaaatagat atggacgcga 420

aagttaagaa aaggtttgca gaaagtagcg aatttagaga tttggctaaa tacaaatcca 480

cagaactaac gaacagccct tttacagaat caaaacacgt agaagattca agcaaaaact 540

tgaatgaaaa ttccaaaata gaagatttag aagttaaggc cctcgatacc gatattagta 600

atcgtttacc tgttattgta aactctcaga aaacacctga agatacattg cccaaacgag 660

ccagcaatca cataccttca accacagaac tggaagatcg ctataa 706

<210> 68

<211> 442

<212> DNA

<213> S005(5)-G9

<400> 68

atacataacc tccatctgtg attattgtga cagacctctg tgaacttgaa agctttcaaa 60

ataggtgtgc tttcgcaaca gatcactaaa atcaaggcaa aatctagaca aaaaaacaaa 120

ctgacagttt aaaatcaaga taaagatctt tatactttta acttcttcta tttctttact 180

atgaagttac atgcataatg tatattgttt cactttattc atgataaatt taaactggtt 240

accaaactag tcctcaacta gttcgaagac actgttaaat ctgtcaagat ttttgatatt 300

tgtttgagtt tctgtttatg attcataata agttaatatt acaatttgtt accgaactag 360

tcctcaacta gtccgaaacc gctaaaaagc gtgttaagtg ttttttgcaa atttgttttc 420

attattcgtt aacgaaatat gt 442

<210> 69

<211> 711

<212> DNA

<213> S005(5)-H2

<400> 69

tgatctgttt gtctggaatg ttctgtatac cagtttataa atattgtgat ttcagattgt 60

gtgtttggat caaagaaacc agcaagtagt tcaggttgga ataacatgct gtggcatgca 120

cgtatgaggg acattccggt gtattgggag atattagaga gatacattct taatgcaatt 180

ctccttgaag cattcaatcc atcaattaaa taaggccgca attctactgg caggaatcct 240

tgatactgac ttaagtattg ttcatcaaag atagacttaa taaatggaag ttctatgatg 300

ggaggagctt tcagccctaa tgaggcatag aatgcgctag ctcgctttgt tacaacttgg 360

ttgaaattgt cacttgtata gtttttccca agaccatggt aggccaatag cattccaaat 420

tcaaaaccct ctctagtggt gtataaaggc acctgattag atacagttgg tgcctgggga 480

aaatcattgg tcccatgttg taaaaaccgt gtcactttaa caccggtgta gactcggtat 540

ttttcaggga aagcaggtga gagataaaat aagtgtgcta gaatgatatt actcctagct 600

tcactctctg tcaaccctaa gcctgaataa taagatatta tctgctgaag catattattt 660

gtgatagtag gtaatgcttg tgcaatttta gccatgtgag tgtatggtag t 711

<210> 70

<211> 272

<212> DNA

<213> S005(6)-13

<400> 70

attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt cgatttcgag 60

cggccgcccg ggcaggtaca tggggacatg taaaacaaca ggttatgggc ccagggcata 120

catttttaaa ttaaagtcat attttatcat ctgtattaaa taaaaagatt cgtacatcat 180

caattgcatc atatatggtg atagtggtat aaatggcgag ctcaacagtg aggattgatc 240

cgctaaacgc ggataactac gatacgtgga ag 272

<210> 71

<211> 313

<212> DNA

<213> S005(6)-17

<400> 71

gttggacatc caggaaaata caacacaata tttgattatc gacaagagga gtgttcttct 60

ggtggctgtt tgactgaact ctgtattcag ctggcaatca ttatgattgg aaaacaagct 120

tttaacacca tactggaagt aataatgcca aagatttggc gaaaaatcat ggccatacaa 180

gtaggtctat cgcgtttgtt caacagtaaa actcatgatg tgaataagga gaaagtggaa 240

cgttatttgc gcgacttaaa attacttgac tggggtccac gcagcttatt tccggagtat 300

ttggaaatgg ttt 313

<210> 72

<211> 560

<212> DNA

<213> S005(6)-49

<400> 72

cacactatta taatttggtc acaacacaat tgggtgatga accctttacg gccggcgtag 60

aacatagtgg taaagacaat acattcttcg atacaaaaga tgaggatgaa gaaatggttg 120

attataaaat tgaagaggca gctttaattg aagaaaatct ggacgactcg tcactttggc 180

cgatattgaa gatgaataca cccgaatggc cgcaactatt gggcggtatg gtgtcgtcgg 240

ttataatggg ctgtgctatg cccattttcg ctttactttt tggtgaaatt ctacaaatat 300

tggctgttag ggatgaccct gactatgtgc gggataacac caatgtatac agtttatatt 360

ttctgatcac cggcattgtt gtaggcctgg caacattttt acagatttat ctattcggta 420

ttgctggcga gagattgacc gaacgcctta ggggtatgat gtttggcaca atgttgaagc 480

aagaagttgc ttggttcgac gacaaagcca atagcaccgg cagtctttgc tcgagacttt 540

ctggcgttgc ctccgccgta 560

<210> 73

<211> 630

<212> DNA

<213> S005(7)-98

<400> 73

ggggttttga gtcactcatc ttcacaacca tgttcataga gtgagcaatc agccactaaa 60

ctcaaaaatt caaacaacaa aagaaatatt tcattcttcg atcgaaaaac aaaggccaaa 120

gccactcgtt tgaaaaagcc gaaatccgtt agtgtatgtt gaacagctgg gactccgagg 180

agatcacagt cgttacagca tgcacacgaa tttccactcg aacaaaacaa gggagtagac 240

caatacaaat ttcgattgaa gaaggaaact cgaatattgt ctgaatttcc aagcgaggaa 300

aactgctccc aatattgttg aagcatagct gatcagatgc gtgactcggt tctatggttc 360

cgaagaaccg aatttgattc aaaattcttg gtttcactac ttgtgattta atggcgttcg 420

tagccatgtg gagccgtgtt agacacagcg tgcggtggaa tgattttgac gaatctgaga 480

agccactgca ctcttttcct cttttggaaa ttataacaat ctttgggcga agactttgcc 540

ctccagtgca gaacgtccat taaaatcgaa gagaactgtg agcaactcag ttcgcaataa 600

ctatatgtat gtatgtatgc ttatacttat 630

<210> 74

<211> 686

<212> DNA

<213> S005(7)-100

<400> 74

atgcttgccg tcaactgctc ctatgcagtt aggaaagttc cataataaat cgaatttcga 60

agcaacttca gtccattctt cttttgttga aggtatcttc aagtaagtat ttcgaagttg 120

actgtaaatc gcatcacaaa cttcaggaac aaattttggg gtagtgtttg gggcaatacg 180

gaaaaaagct gaaagacttt tgtagctatc gcccgtggcc aaaaagcgga gagtgcaggc 240

caaacgatga cttgctggaa tagcttcacg aaaacgtgta tctttttttt taatcagtgg 300

ggaaattagc tgcagtaact cattaaaatt gttttcgttc attcgcacgt aatttttaaa 360

aagaaacttt tgattatcaa cttctcgaaa ttcacgtaaa gtcgtttcat atgcaccatt 420

agtatttctt ctttccagcc aatttctcac ccagatacta cgttgtttag gatttgtttt 480

acgtttctga tttttctttt tcagctttaa tgttgcaatg gtaagcagag caactgcaac 540

ttttttcttc ttcaaataat ccatgttctt ccacatcaat tccgtttatc gacagcggat 600

gtttcacagc aacacatcct tcaactttta cgctgccaaa actgtttccg ttatgccact 660

ttgtgtttgc ttgtttttat agtttt 686

<210> 75

<211> 537

<212> DNA

<213> S005(7)-103

<400> 75

tcttcgtttt gtggcagact attaatactg tcgcctttca atggtatatg tataacatca 60

gcggtcaaac gtgatgggcg ttttcccgaa acgctactta cactgcgacc aactgggcgg 120

cttgacttag ttcccttatt aaattcactt ttagaggggc ttgttggtaa agatataggc 180

tgaagagatt ttacagatcc gcgatttgga tcgttcattc gaaattggga aatacttggt 240

tcacgactta aatttggagt agtgcccttg gaattttggg ctacgtctcc actttgattg 300

aggtcaggcc tgcttttacg tcgcgaaaaa atcttggaga aaaatcctga tttcttttgt 360

ttaggtaatg tatttgaatt actgccgttg atcggcttgg agggcaaacg ctttgaaagt 420

ggcgactgat ctggagtctt ttttattata atcggagctg gtgaacgaga tctgtaatcg 480

gatacaacgt tgatattatc ttgaatcgaa ttgctttcag taattccatc ttgcttt 537

<210> 76

<211> 517

<212> DNA

<213> S005(7)-116

<400> 76

agcatttctt tatgccttat actttgccaa tatcggttta attacatcct cggttacttt 60

cagacctttg gcgtttgctt tctcataata tggcaactgc ttcatgttct ccaaccacgc 120

tgtcaacttt ggccacttgt ccgccaccac tggcacaatt aaattcagaa cagtaaccgt 180

tgttagaaga ctgaagtcag ctatggttaa atgatcagca gccatatatt tattttgatt 240

gagataagcc tccagcacag tgtatacatc gctaatgcct tttaatgctt catcaaccgc 300

cgcctttggc agttcgcttg taagtaatgg acgcaaacgt tgtttcattg tttcgttaaa 360

gataacacca ttctcatagt gcaaacgttg atcgacagtg gcgcgttgat acaggtcctt 420

tggatagagc gaatcatctt tgccatattt gctagccaaa aaggcgataa tggcgtgaga 480

atcggatata tatttgccat caacttcgag tacctcg 517

<210> 77

<211> 719

<212> DNA

<213> S005(7)-107

<400> 77

ttgtaaaatc aagaccagta tagaacatct tgtgtttcct gaatcggttg cgaatcttct 60

gggctccacc agttgcagtc tccaatccat aagcaattgg agtcaaatcg ggtgaaaggg 120

cctggtatcc ttcaatgagt ggaatagcga atacggcctc tccaaatgtg atagtagctg 180

gatatcccca aacagcacga actttatgct catcaagtcc acaaacatga gcacggacga 240

aagcgcaaca atcggggaag cttatgtcat caccagcttt cacaaaatgc caaaattttc 300

tcacttcctg tattttgtct gggtcatcac gaatttgtct cttagtttta acgccatatc 360

gagtccacgg aagaccagga cttttactcc agatgtcact ttctctttta aagacatcat 420

taaggtgtaa cattttaact gcattgggca acagaaattt tcgcgtagca tgagcgattg 480

ataaatcaag taatggatca ttaggccttt tagtggtatt attaaattgt tttaagtcgt 540

cgataataag gctttgcgta acgggtgaac gtcttttagc aggaatatga tccagaaatg 600

gtaacacgaa tttacttgga aatatctcgt catgagtcat tgacgcgaat ccttgtgttc 660

ttcctatata gcgtaacggc atggtttaac tgtttaacag tagcgttgtc ggcccatgt 719

<210> 78

<211> 718

<212> DNA

<213> S005(7)-131

<400> 78

agaacacaat gaaccagatg ttgctgacga atctaaaaag aaaatttttc gagctttgtc 60

gaaatcattg tctgcagagg acagcaattc agaggattgc aagaattcgc cgcaccataa 120

taaaatcgga attttacaaa atgcaaattc aatgtcaaat acagcatcct taaatacgtt 180

aaataacgaa aataaccacg taattaatca gtttgctgac tctttggatt tgaaaattga 240

atgtctattg agagattggc atcaaagtcc cgatcttgta tttgcaattc atcctattga 300

tggaagttat cttatatggg tagttgattg gttggacgat tactatcccg gatcttttcg 360

ccaagcacaa atttcgtttt ccacaagaat accttttgca tttcctctcg gagatgcaat 420

gtcgatgtca acaacagtta gtttatataa tacgggatct ccaccactta tttttagaga 480

aatcgcaaat agtgtgaaaa gtagaagtga ggaaataaca tcacttaaag agtcaccaag 540

ctcttcgcct ttaaataagg tcagcaaccc cgacataagc gatcaagctt ttaagacttt 600

aacaagtagt atttcaccgt ctgtttcaat ggtgacaaaa cattccaatg gaacattaaa 660

tctctggcaa ctaacttttg cacttaaaac taagttcacg caagttctaa gcataggg 718

<210> 79

<211> 710

<212> DNA

<213> S005(7)-176

<400> 79

acgaagtcta tcaaaagttt cgtggtaaag caaagatagt cggcacatat atatacacta 60

agccaattgc gattgtagtc gatctagatc tgataaagtc gatattaata aaggatttca 120

ataaatttac cgatcgtatc gaacgcggcc atcacccgac gaactcatta aatagcaatg 180

ttttttcact gcccggtgaa aaatggcgtc cactacgcat aaagctttca ccaaccttca 240

catcggcgaa aatgaagttt atgttttcca cgctgttgac ggtagcacaa caattggagg 300

aggcattcag caaagagttg tccaaaacaa cgagtggtgt tattgaatta cacgatataa 360

tgggtcgcta tacaaccgat gtgattggtt cttgtgtctt cggcattgaa tgcaatagct 420

tgaaggatcc gaatgctgac tttcgtcgca taggacatac aattttttat cggcagtatt 480

actccatacg ttggcgcaca ttcatgctca cttatccgat tatgtttaaa atactacagc 540

tcttcaaggt gaaacgtatt cccaatcatg tcgaagactt cataattcgt ttggtgcatg 600

acacagtgcg ttatcgtgaa gagcacaagg tggagcgtaa agatttcatg aatctattaa 660

ttgaattgaa aaatacaaag gactcggaag gtgtgcctct aatgagtatt 710

<210> 80

<211> 700

<212> DNA

<213> S005(7)-178

<400> 80

ttcagcgagc acatattatt tttttggctt ttccttaaat aattttgttt attgtcttct 60

tctttggaca ttgtaaaatt gcattaattg gaattgtaac gcatacgcca tggtgttctg 120

gacgttagtg atcggtgcga tcataatcgc ttggctctac aagctaaata aggattatgt 180

ggtgctgaca ttatttacta agcgagtgac aacagttgat ggcacgccct tggagaattc 240

agtggcgctg cccaaaggac tcacaatatt tggcaatgcc ttcgatttgg ctgtatccgg 300

cgatgacatc ttcgacttct cgcgcaaact agctgtcgaa atgaaacgca gttatctaca 360

atatggacta ttcacatctt tttaccacat aatcaccgct gaggatgctg aactagtgat 420

gaacgatcaa aacaatctaa ccactaaggg tattatttat aattttttgg agccattcct 480

aaatagagga ctgctgacgt catcgggcaa aaaatggcac tctcgtagaa aaatgctgac 540

tcctgccttc cattttaata tacttggaca atttgaagag atttttaaag aagagagtaa 600

aaaatttgtg gagagcttgg aagccagcga tttaagttct gtaactttga acgaaatcat 660

accaaaattt acactgaacg ccatttgtga gaccgcattg 700

<210> 81

<211> 846

<212> DNA

<213> S005(7)-181

<400> 81

aacgatcgga acgtagttgt gaattgcttc ggcccatact ggtggtttgc tgattctcga 60

cgaggcaaga accatcattt gacaagcacg gctgacccgc attcgaacag atgccgaacg 120

tataattttt taaaggcatc gacaacttgt atgtcttacc ctgcagcgat cgcataccca 180

aaacttcatt gccatgggtt atcgtggtgc atggtggatt gttattacag gccaaaggcg 240

tggtaaacga aaggtcatac acgttttctt tggcgccaat aaatgacaat tgagaactct 300

ttatctcatt atcacatatg aagtgtattg ttgaggagta attatgtgtc gcattataag 360

gtgttgggga tggcaggcgc atgagcaaat tgccattttc taatatagga ttgggctgca 420

cggtgccata gtcaacaaac cgttttttgt agtcggtgga gtttaatttt actaaacaca 480

cactactcgc tttggggcac attgccatct cgccatacag cactggcttg caaatgttca 540

ccgtaaatat attgccatgt cgatcgcttg ttctatagtt aaagtcactc aacgcaagca 600

gatcataatt gtgtccagta acggggtcct gcacactgca accactcgat ttaaccgtct 660

cgggttcagg caaacaggca tatggtgtct ctaacgatat atagaattta cacgcattgg 720

cggaatataa tgtcactttc gattgtttag catccagtgt gtagtcacag ccaaggaaca 780

caaacagttt aaactcgcca tcgccgctgg cacacttggc gccattgcta aattcaaagt 840

acctcg 846

<210> 82

<211> 649

<212> DNA

<213> S005(7)-188

<400> 82

gttacagaag ttcggcacaa actaagactt cttttataaa taatattttt cttgagtgcg 60

cataatgctg tcggtaaata ctgcttgcct cttggcggct ctcttggggt tggtctatgt 120

ttggtgtcgc tacacatatg gctactggaa acgcaataag attccctata tgacgccgct 180

gccactaatt ggcaatatgg aagttttgtt taaaatgagc aatagttttt atctgtatct 240

atctgacgtc tataaggatg ctaaaatgtc aaaggctgca gcagtcggca tctatatact 300

caacaagcca gcgttagtgt tacgtgaacc ggaacttata aaatctgtgc tcatcaagga 360

attctcaaaa ttcgccaatc ggtcagccac ctgtgatcca cataatgatg ctttgggttg 420

gaataatcta tttttcatac gaaatccaca atggaaggat ttgagatcga aaataacgcc 480

ggttttcaca acgggaaaga tcaaacaaat gtatccacta atgactgaga tcggcacgga 540

gttggaagcc catttgaatt cgtatgcgaa gactaacgac gctttcgtaa ctgaagttaa 600

agaggtttgt gccagcttca ccaccgacat gattgccacc atcgctttt 649

<210> 83

<211> 710

<212> DNA

<213> S005(7)-189

<400> 83

tttatattgc gctaattttg ccaaatcagt cattattcct tgcttcagca aaattctata 60

catattatta cgttgtaaat gcactttact tgacagcatt ggactttcaa tcattatggg 120

aatcaaacca ataagaactt gcaaacgaac atgtttttct gtgtagctag gttcagttag 180

tgttcgcaca atcgcttcat gaagttcttc aaccaatttc gattgaactg ttggctgact 240

aacgcaatct gacaaagaag aaagtagaac tcttgacatc atactcactt ctggatcttg 300

ttggcgctta gttattggta gaaaatgatc tagaataaat gagatgcatg tctgtggttt 360

tttaattagt gggctgtcgg acggcatata tacgtattcg ataataattt tcggcactag 420

acactggtag gatctaactg catctgctaa tatttttaat aaagtcgaac gcggcaatat 480

tggcttttgg tgcgtatgac ttgtagatgg tatatgccaa ctgcagcagc aaggatttac 540

atcaatattt tctgtgtgtt ttttagccga agatatttta ggttgagaat tatctaattg 600

aatatgtgaa gtctccccca tccctgtgat actatcatca caatcatctg ttgtggacaa 660

tccgccataa tgttcacaag gttgaattat agctctcaat atatctttta 710

<210> 84

<211> 641

<212> DNA

<213> S005(8)-200

<400> 84

ttcggagata atataccatg gcgcaaaagt ttaaacggca gtgatacttc ctgtataaaa 60

aacattctta taacccaaga tgtccaatta agtagtattc atacggtatt tggaacagtc 120

gaattttgtc agatagttgg tgttacggat gaagaacttg aacaagcatc tcgttggaat 180

ggccgtggtg ttctgaatta tttgcaacaa gacatacaga caggtggcaa atggtggatt 240

acaaacatga atcgttcgct aagtgtattt gagctttgtc ccgaattgct attaaatttg 300

caagatgact tggaaaaaca aggatctgat ttagctggag ttaatgctga atttactttt 360

cgggaaattc gggaaagacc ttgtataaaa aaagaaataa actcagaaag tttaaatgaa 420

caatgcgtat ctgatgaaaa taattgcgtg gtaagaaatg aagtaaagtg tgagggtttc 480

ccacaatcaa tgtcaatgag tagtagttca ttgcataaat cgtgtcctct tagatatcag 540

ccatattcgg ctgactcaat atcattagat ggaattgaaa tcacttttgg ctccttatgc 600

agcaaaatat cttatattag ccataaaaga tcgggttcga c 641

<210> 85

<211> 671

<212> DNA

<213> S005(8)-194

<400> 85

gatttcggag aacgcattat cccaggtaat tgtggcccaa ccctgtggtg cttgattatc 60

gtgcaccaca acaactatgg gtagactgag tgccgagcaa ttaatcgcat atcccttata 120

taccgcggtt gtataaaaga gcaaagcgta tttgccatct gcaacaattt cggaaccctt 180

tttctcggag cgtttgttgt cgtggattat aagagttgaa aaatttgctg aaaaactcct 240

attactggca tcgaattcca tttggcactc atttcctgtc attttgccat tgcattctgg 300

tggcatgtga atattctcaa tagtaagtcg ttttgctaga gcctctgaga gtatttcaca 360

ttttacaact ggtttgctca aatgaatgcc caatttatca ccgatcaacc agcgcacagt 420

tgcctgaagt ttggaaccct ttttctccac ccgtggcggt tgcttttcga cgatgaatgt 480

tgaaaagata agtatgttat gtaaggtctg tgcatgtgtc aagaactcat taagtgttgg 540

gtcatctcca aaatgcagca gattacttat aaataatgta atgtctgcca tgcgatccat 600

caaggactcc acaacaaatt gtataccatc taaattggta gggaacggcg caccattgcc 660

agccaaggcc t 671

<210> 86

<211> 376

<212> DNA

<213> S005(8)-211

<400> 86

aaaattttca cacgcaatgt tgtggtccat ttcggataga agaatgcgat aaggaattga 60

atagcgcgac ctaatgtaaa gtctagctgt tttcggccta gcgcacgaaa ctcggcattc 120

ggatctataa gactattggc tttaatgcca aaggcggtgg tggcaatcac atcggtggtg 180

aagtttgcac aaatctcttt aacttcagtt acgaaagcgt cgtctgtttt cgcaaacgaa 240

ttcaaatggg cttccaactc cgtgccgatc tcagtcatta atgaatacat ttgtttgatc 300

tttcctgatg tgaaaatcgg cgttattttc gttctcaaat ccttccattg tggattgcgt 360

ataaaaaata gattgt 376

<210> 87

<211> 657

<212> DNA

<213> S005(8)-212

<400> 87

atggggagtg ttattttgga agtgcgttac agaagttcgg cacaaactaa gacttctttt 60

ataaataata tttttcttga gtgcgcataa tgctgtcggt aaatactgct tgcctcttgg 120

cggctctctt ggggttggtc tatgtttggt gtcgctacac atatggctac tggaaacgca 180

ataagattcc ctatatgacg ccgctgccac taattggcaa tatggaagtt ttgtttaaaa 240

tgagcaatag tttttatctg tatctatctg acgtctataa ggatgctaaa atgtcaaagg 300

ctgcagcagt cggcatctat atactcaaca agccagcgtt agtgttacgt gaaccggaac 360

ttataaaatc tgtgctcatc aaggaattct caaaattcgc caatcggtca gccacctgtg 420

atccacataa tgatgctttg ggttggaata atctattttt tatacgcaat ccacaatgga 480

aggatttgag aacgaaaata acgccgattt tcacatcagg aaagatcaaa caaatgtatt 540

cattaatgac tgagatcggc acggagttgg aagcccattt gaattcgttt gcgaaaacag 600

acgacgcttt cgtaactgaa gttaaagaga tttgtgcaaa cttcaccgcc gatgtga 657

<210> 88

<211> 603

<212> DNA

<213> S005(8)-213

<400> 88

gcagaattta atcttttcta atggccaatt cagccagccc gtagtaatgc aaaaatatgt 60

ctcatgcggt gctacgtgat gtatgcgatg atgctttcgt ggtaatacta tgtgatagtt 120

ttgcaagaag agaacccatc gcggtagacc ccaataagta tgcgaccatt tgtgaatctg 180

atttgtcata gccacaaata ttgaacacaa aaacaaatat gatatccagc caaaatcttg 240

ctggatatct tgaggtgatt tcgtgttaaa gctgtatgct aagaagccca atatgggtat 300

gctaaccata aagttgtcac cattggtttc gatgaagtca tgccgcgtta tagacgttgg 360

atccaaatga tgttcacgga aaggtcgtaa aaaattcttc ccaatgaccg gtaattcaac 420

gctaccccaa gtatcagctg cccagtggac taatccggaa ccgaagtcgg ccgttataat 480

accacacaat gctgatacac aagccacact cagtctttca aaatgtatat gttttaggat 540

cagtatcagg tcaactaaca tgagtgcgat gcaggtgtaa acgcagacta tctcttgtag 600

acg 603

<210> 89

<211> 739

<212> DNA

<213> S005(8)-216

<400> 89

tgggtattac tggctcatac aaataagctg tagggcacac attgtgaaca aaatcagatc 60

taacaaaacg cttaacaata ttcttaaaca atggcatatc atttggtaac ttacacataa 120

ctattgcact attcttcaat ttatatttaa caatagactt ataatctatc ggtttataca 180

tttgttgatc acaataataa aacttcgcat tttcactcat tgtgtcaaat ttgtcaaaat 240

aatgatctat agctaaaaac cacctaccct taactccaag acaccttgca acagactcat 300

aattaccatc atgcgcaaat aagtagaaat aatttcgttt taaatgtctg ctcaaactat 360

taaagaactc agtattcatt tctggttgaa tagatttcga ttctatcgtc tttgatgtta 420

tattaagacg tggttttgta ttcttcttat tctgatcata agccatttga ggattgatac 480

aaatttggca ttgtccattc aaacaagtct cagtgacaac atgttcttcc aacatcttgg 540

catatgcaac attttgattt ttaatactat tatatggatt taatttttga acttgtttaa 600

ttacgctctt gtaattacaa actccatata atactccaac tgctccaata acaacaagag 660

aatatttaca aatcttccac aaaagactac ccttcttcgt catccaagta tctggatcaa 720

cctcttctgc taaaaaact 739

<210> 90

<211> 545

<212> DNA

<213> S005(8)-224

<400> 90

tcaatttctg acgcatcaac aagccttttt cttcatcaaa tttgaagaag aaatgttcta 60

ccagccagtc cataacagga catcttctgt ggtagattgc attatcaagc tcgagttgtg 120

ttccacaatt tggacattga ctttgttcat tacgaatatc cgtaaataga acggccaaca 180

cctcatcttg cgacaagtcg atttccgata atgtttgagc gagcactttc gcacttttgc 240

tctttatgtc ttggattata gaatccttga cttttgagaa taagtttagc tttgcagctg 300

ctttttctct tggctttgga gaaaatacta gtttcaccaa ccattgagtc tgttgatcat 360

ccaacttata cttcggaact aacacctttc gctttgaagc aggtattgat tcgtcgttat 420

tgttagaaat ccacaaacct tgagtcgatt cgtctaagta aatttcttta ctgccatata 480

gagcgtccac tctatttaac aacttatctc taagcttctc ccacaatagt gcttgtaaca 540

aaatc 545

<210> 91

<211> 683

<212> DNA

<213> S005(8)-235

<400> 91

gacaagatac cggcgaattg atcctgaagt gcaccggccc cagcgaagcc gtgatgacga 60

tgtagagctt agctctatta atggttcaga gagagattaa gttgaatatc tctaaacagc 120

aatatgaagg aaaacatcaa tgaataaaac cctcagtaga tgagtaaaat atgaaggaaa 180

acatcaatga ataaaaccct cagtagatga gtaatcaaat tgaatgaagg aaaacatcaa 240

agaataaaac cccatgattg gtaatcatca atatgaacca tccctttcac ggtaagggaa 300

ctatgttggg cttagtttca gttcaaccta tccaataacc gaaggaaaac gaatttaacc 360

cgttgattac gtaaaatcac tatcgaagga aaacgttata acccggaatg tccgttaata 420

tcgaaggaaa acgaaatata acctggttgc tccattaccg cttacactcg aaggaaaacg 480

ttgatataac ccggttgcca cgttattagc actatagaag gaaaacttta acccgatcgt 540

tacaatcgta attatgtaac attcgaggat tctcctctgt tgacatatgt ccgtctgatg 600

aacgaaacca tcacatgaag gaaaacatta taaccactgt ccggataaag acagtaaatg 660

aaggagaaaa aaaaaaaaaa aaa 683

<210> 92

<211> 609

<212> DNA

<213> S005(8)-236

<400> 92

tatatgcgga gcaactctgc gtagggtttc gctactaagt attacacccg gaccacccat 60

acaaaaattt tcatcaaact ccaatgataa taagccaaat tcctcactat tgcccttgcc 120

ggcttgacct ataaactgtg gcttagaact gtcgatcgag cgtagaaagc gttcgagttt 180

ctccggttca atgtatacat cgtcatcggc tcgtatgaac cattcaaact tgtcaatata 240

gtgttcatac atgtaataga gcatcatgaa tgatttcttc tgcggtggat aacgatcgtc 300

tacgttcttc aaacctatta ctggcaattc gttggagtga gaaccttctg aactaaagaa 360

ggctattcta ccaggcacct ccttccccca tgtatcataa actgcacgtg cacgtccctc 420

gataaaattc tgcgctgtca ttacgccgac gaatacaaga ttcctctgtg aggtttttat 480

tgtttcttct tcaggattca taccgatgat ttcgaatggg ctttgttgcc cgcttggctt 540

catgctgcac cgtttaacga tctccagcgc gcgatagttt ttgagaaccg tgccaatgca 600

tagtcccaa 609

<210> 93

<211> 525

<212> DNA

<213> S005(8)-254

<400> 93

gtcaactttc tttaccgtga cgtgagaact tttaccattt tttggaatga acgtaactct 60

gacatttctc aaagccctgg taaattccag tcttatgaag ttcgcgtaga catatgtcag 120

ctagtttcat gacaccttaa tagcctgttg caattgcgct ggggtaatgc cagctggttc 180

ctaggatttt aatgatttga acaagtcaag tcacgtgccg tttatgaaga aagtaggcaa 240

aagcgactca atcctcttgt atcactccta gaggagtgta tttcctagaa agtttttctt 300

tctattgaca tccctaagat aacgaaggaa tggggttcct tgcgagaatg cgcccgagca 360

tagaggactt ttgactgtcc tctactttct cacagaagct tttctataag gtcttcttga 420

gctttcttat cttgcacttg tatattcata aacatgtttc atataaaatg tcatttaatt 480

tcccccggat ggccttgttg aataagcgtc tgcaagattc cctga 525

<210> 94

<211> 583

<212> DNA

<213> S005(8)-259

<400> 94

taccttcctt aatgcaggcg cgcagataag gaagattttg catattctct atagttaaac 60

gggagtcctt atttggcaaa atattcttga gttcttcgaa caatttttgc tgtttgtctg 120

gatttttggc gatgcagaat aatatgcccg ttaaggtgga acttgttgta tcaacaccag 180

ccatcatcat atctagagcc ataacaacgg cgatgcgacg atcgatgcgc aaaagcttct 240

ccaaaacact attttcctta cccacctcag aggtgaattt tccatcccta tccatttcaa 300

tttgttctag cgcctcatca atatacttgt tgcacaaatc tgctagcaaa tccaaagttt 360

tcattaattt gtagaatttc ggtgttttca caattttcca aattgaaggt ttcacttcta 420

aatcttcgga gaggtcgaaa aagtcccgta tcgattgcaa cagttgttta cattctggag 480

agtctcgatt cttgtgtatc atacctaaac gggtgtttag tgcaacaccg acaatgcctt 540

caagcgttag acgattaaga tcatcgtgaa agtcatcggg cat 583

<210> 95

<211> 601

<212> DNA

<213> S005(8)-261

<400> 95

ataccgtcca gttggagagc attgccgtgt ctgaaggctt ccataacaca ctacagccca 60

tcaaagcagg tgtaaacgct aaattaccac cgattgcggt gaaattaaat ggtgttattg 120

ctgcaacaaa tccctctaag ccccgaatgc gtaaatggtt tttggttatg cctggctgag 180

gacttagagg ttgttccatg gctaaatttt tcaagtaacc ggcattcatt ctggtaaaat 240

cgatcaattc agcacctgaa tcaatttcag tctgtattac tgtttttgat tgccccaaca 300

ttgtagtggc taacaaatca gcacgatact ttttggccat aagatcagca gctcgctccc 360

aaatttcaat acgtttttta agaggggttt tatcccattc cttttgtttc tgcaccgaga 420

cacgtatagc ttcttctata gtagcattgt cagcatagta atattttgca agtttgtgat 480

tcttgttatg tggcataact tggaacgtct cttgtccctc tcttatctct ttaccgttta 540

taacaatcgg tatatcttca caattatttt cataatgttt aagggctttc tgcaaatctt 600

t 601

<210> 96

<211> 609

<212> DNA

<213> S005(8)-286

<400> 96

gccagtggca gtgtgaagag tagctgtaaa aatttttata cataagcagc taaaggccga 60

cttcattaaa agaaaaaaca aaaaatctct atataatcga agtaaaataa ttcttgtaaa 120

atttgctata atatacatgg acattgcata aaaggtcaat agaacgtaaa aaagtgtgtg 180

tagcgacaaa agaggcgaga aacgtcgata ttttggagtc gctctagcgc tggcagtggc 240

ctgtgaaact tattgtcggc gcaaccagac gaaaagcatc tatctagctg ctgtcctctt 300

ctctgatagt tttcactgaa cttggcagac tcactaaagc tatctctagc tcttgacgcg 360

tttaaaagag acaactgtgt ttcaaccaca ataacaacaa caaaagcatt aactatacta 420

acttcaacta aagcaagaac ttttacaaca cagctgagtt actcgaactt tgctgtgtct 480

ttgctcgtaa actttgcagt taaaacaaag aactcatcga aaacagcact tctcatcgtg 540

tgaacgcaac gagcatcttc cgtgagaata ttgtttcgaa atatccacca attaaacagc 600

agcaacaaa 609

<210> 97

<211> 338

<212> DNA

<213> S005(8)-264

<400> 97

attggatttc cgataccgtc aatgaaaatt ctttcataat aaaacctgcc gagttaaata 60

ttattcaact tttaaatatt tatcactcaa ctgaaacaaa aaagttaaag ccatggaatg 120

cacaaaagag atcacatgtc tacagaactt aactcaacaa gttgctgagt tatcaaaagc 180

gttatcccaa aatatgcagc atacgttgtc cctggaggga aaattttcag ctgccatgga 240

agaaatcgct aaactccaac ggcaaaacgc gatgatgcta gatgtcatca ataaaacctt 300

atatagtaat agaaaaaaaa aaaaaaaaaa aaaaaaaa 338

<210> 98

<211> 640

<212> DNA

<213> S005(9)-293

<400> 98

gattagcgtg gtcgcggccg aggtacatgg gagaacaaaa tttttaattg tcaaagtgac 60

attgccttat ctaaatgcaa acatgtttga cgtttgcaat gattaatctg tgcaaaaaag 120

taataattta tagtttttat taaacaattt tattaaattt cacggcagcc gtttctaatt 180

tcatcagcat tcggggtaac attatgccat tgggagctaa ataacactta cataggcaat 240

acgggcgcgc cgtaatgtca gtgagagcaa tacatgagtg taaagaaagc cattctatgt 300

aaggcgagtc tacgtagctg gaacccaccc agattttcat acggccaagg actgccaact 360

aggccggaat caaaattatt tcaggaatgc ttttcgaagc tacaaaacaa caagagttta 420

aagaagcgag cgcgaaacta caactgacgt aacagagctg aaatgacctc accatcctaa 480

cgaggattga ataaccgtta aaacaaccac aacaaaagct taaatgaact tatgtcatcg 540

gtcgacaaac tgttatcata gtgaaattac cgttggaata agtgttggac gagctaaact 600

ttttttacca ttaaagtaac gatattttta tttatttatt 640

<210> 99

<211> 345

<212> DNA

<213> S005(9)-299

<400> 99

tcggagtaga ccttcctccg aggtaatacc aattataagt ggtaaggaga agccatgtgg 60

catgcctcca tcacgtggta agactgtaag gaaggcaccc tcatgtggag gctcaacaac 120

aggttggaat ggtatcacgg gatcaaagtc ccattcgaag aagtcgtaga gcgtagcaac 180

gacatcgctg gcttctttag tgcgtaagca gcgcaatatt tgttgccaat tttcctcagg 240

attacaccca accaattgtg cgaccttccg tgcacgcttt gcgggtgtgc ctttatgctc 300

tggttgtccc cagggattgt agtatgaacc ggactgtacc tcggc 345

<210> 100

<211> 914

<212> DNA

<213> S005(9)-304

<400> 100

ggagtgcgtt aagaagttcg gcacaaacta agacttcttt tataaataat atttttcttg 60

agtgcgcata atgctgtcgg taaatactgc ttgcctcttg gcggctctct tggggttggt 120

ctatgtttgg tgtcgctaca catatggcta ctggaaacgc aataagattc cctatatgac 180

gccgctgcca ctaattggca atatggaagt tttgtttaaa atgagcaata gtttttatct 240

gtatctatct gacgtctata aggatgctaa aatgtcaaag gctgcagcag tcggcatcta 300

tatactcaac aagccagcgt tagtgttacg tgaaccggaa cttataaaat ctgtgctcat 360

caaggaattc tcaaaattcg ccaatcggtc agccacctgt gatccacata atgatgcttt 420

gggttggaat aatctatttt tcatacgaaa tccacaatgg aaggatttga gatcgaaaat 480

aacgccggtt ttcacaacgg gaaagatcaa acaaatgtat ccactaatga ctgagatcgg 540

cacggagttg gaagcccatt tgaattcgta tgcgaagact aacgacgctt tcgtaactga 600

agttaaagag gtttgtgcca gcttcaccac cgacatgatt gccaccatcg cttttggtgt 660

aaaagccaac agtcttgtaa atccaaacgg cgaatttcgc actcaaggac gaaaattgtt 720

cacctttaca ctccgtcgtg ccattgactt cttcatcgcc tttttcgtcc ccaagtgggt 780

ctctacattc cgtattaaac tttttacaac agagttcagt tcctttttac gcggcaccat 840

cgaacatgtt atgacgtcag agaggagagc aaagcaacgc gtaacgatct gatcgatgta 900

ttgtgagcct taaa 914

<210> 101

<211> 588

<212> DNA

<213> S005(9)-308

<400> 101

tgtagcatta ttattgttgc cgttagccgt ggaagtgtca ttaataatat tattgtggtt 60

attgttgtga tagcgcgtgc cgtagaaact gttgcgaaag ggttgttggc gccccaaatt 120

attactcgga taattattgt tgctcgtcaa caagtgatta tcattagtgt tgttgttgtt 180

gtggttatta ttattagtat taacgaaact attatcttga cttgaataca tcggcgtaga 240

actattgtga ccatataacg ggccatggtt agcattacca ttgctattgg tattatgagc 300

gtgaccgctg ctactgttat tggtgttgcc tggaaaagtg tgtaaattac agcttgcgcg 360

agtcgctcga aacgaactat acggcctaga cgtaaattga tgtcctaccg gcattttcct 420

agttattttt gcgccgagag tttaatatcc gctaactaga tgttcttaat ttcttagttt 480

acacttatga aatgggtgca ccaggttgac aaagggtcct tgctggtagt ctggtttatc 540

acatttcaat ttgtcactaa tcaataatgc ttaaagtatt taaaaagt 588

<210> 102

<211> 707

<212> DNA

<213> S005(9)-310

<400> 102

tacccctgcc actgataaaa acacagatga tataataagt gtggaaagac catttaccga 60

ccaatagagc gatatgccga gagtgccaga tattaagagt ccataaatta gtaaattttt 120

agctcctata gctcgcacag taataccagc aaaacaatat gctactacac cagccgacga 180

tacgacaata ttattaacgt atagatctat tgacacgttt ggtaggttac tacaggagtc 240

gtggaaatct gtaatcactg atgttttatt aacactatac tctagaattg cacatagatt 300

cgctgagctt gattcaccgt tatctagagc ttcgtattca gcgatcgatg caaaaagttg 360

aggcaaccac agtcgaacag tatttaaacc aagtaaaata cagaattgca tcgcatacgc 420

gtgaatggag tgacctaata acggctttgt gaacataggc ttaatctgct tcattccttc 480

cttgaaagct tgggagaatg ttctgggctc acgtttgtat ttctgcttaa ctggtttgtt 540

ttctacagta aatataagct cattaacatt cgagatacga ttcggaactt cctcaaccag 600

ctctttgact ggataagcag ttctgcttgt tcttttatta atagaataaa tcttttgaaa 660

tgctaggagg gcctcagcat ttcttccttg agacattaaa aatttag 707

<210> 103

<211> 671

<212> DNA

<213> S005(9)-312

<400> 103

tatggccgaa cgaagatatt tagcaaaata ggagacaatg aattgtaatt ctgatgcgct 60

tgatgattca cacgttcgct atgtgtggcg ttaaaatgtt ttattacaac tacttaaatt 120

ataaatgtgt tagataaatt gtaaacactt tgtaaaagtc attaaaacta aaatatttgc 180

taaattgaat gttatgtaaa gcataaatac cgataacaca ccttacaccg taataagaat 240

gtaaataact aattgtttag aaaaggcgaa tcactgtaaa aaacgcagca aagatgtgtt 300

acttcgtgtt ttattcgttc attgtaagat cttgtaaatt atatcaggtt tctgtgtctg 360

ctaattatac ttttgtttta ttttatcaaa aaacttaaag atttctctga gcctagataa 420

cgcagcccat caccagtcgc agttgcgatt aaacttgttt attctcacat gttttagttc 480

ttaagttttc aagtttgaag tcacagctta aaagcacaca tatacacact tacatataga 540

gcattccaac ttaaagttat ttttaattaa cgcaataatg ttttgttcat gcatgcatgt 600

tactcaatgt tcaagtatta aaaaacctat cccaactcgt ctcacacgca caaaaaaaaa 660

aaaaaaaaaa a 671

<210> 104

<211> 506

<212> DNA

<213> S005(9)-317

<400> 104

accaataccc acatcaacaa caacaacaac acccaacaca atcgaaaaca tcacgccaac 60

agcaacagca gcagcaacag tcacagcaac atcatcacat tctccctact tcggcaaacg 120

gtatgtggtc gtggcgctca accgacgcgc ctttagtttg gacgaggctg gcaattgaag 180

ccgcagcagc tgtagcacga agtattatgg agctttcgac gagttttcat ttacttttgt 240

gctgattttc aatctaaatg cgtagcacag ttatgtgctg agtgttctga aattattgac 300

ttccgaagtg gcggttggcg cagcgtaaac ataaggcgta actgtgcggc caacgagctt 360

tcattttatt tcatactgat ttcgaaagtg ctcctgctca accgttatgt gatctagttc 420

cagatctaac gacttccagc acgttttatg gctaaagttg gcgctcgggc gaccaatctt 480

cactcatcca tggcatgtac ctcggc 506

<210> 105

<211> 611

<212> DNA

<213> S005(9)-329

<400> 105

cgcctgctaa agatttgaaa ggagccttag agcctaatca gcacctagat ggtgccgaac 60

gcctcctcga agggcgtgtc tatggcccag aatgtctcat tgcacgtaaa aatgagatat 120

acaccggcat acatggtggt gagataatta aactaactgc cgatcatgtt acacacgtag 180

caaaaattgg acaaccttgt gaagaaatat ttgaagaagc tcgttgcgga agacctttgg 240

gccttgcctt tgatacgcag ggtaataatt tgattgtggc ggacgcatac tacggcattt 300

ggctgataga tcttaatacg aatcaaaaga aattgctggt atcgccacaa caagaacaac 360

ctggtaaaga tattccacgt gaagctaaaa ttttcaacac ggtagccgtt gatcagaaag 420

gtgacattta ttggacagat tcggaatcag attttgtgtt gctcgattta gtatttggct 480

ctttggcaaa cccctctggc cgactcttca aatatgatcg tgctaacaat gttagcgaag 540

ttttgctgga cgaattattc tttgctaatg gtttggccct aagtcccgat gaagacttca 600

ttgttgtcgc c 611

<210> 106

<211> 668

<212> DNA

<213> S005(9)-333

<400> 106

gtcttgaggg tttcattgag agtcaccatg ctagactcaa tacttgattg tattgatcca 60

aatacgtttt cattcactaa ggacttcaat attgaggtaa ttctagttat gtcatttcga 120

ctcgctgaaa cattcattac tgggtcaaca aatctatgaa ccatactata ggcacactcc 180

ttatcaatat aagcccaaca ctcttcccaa gatcgttttt gatataactc ttgatactgc 240

ttgatctgat taggtgccca atggcttttc attgcatcca ggacatgaga tttatctaat 300

atcgatacca gcctaacaat aggtgcctca catagaggga gatgtagttg tccggaacta 360

gtgcaaggga gagctgcgtc ttcactcttt ggagaagtgg taagattttc tccttgaact 420

ctttccttac attttccctc acatttatct agccctcccg ttattctgga acttttaatt 480

attgcctcat tcttttctac tctcggaatg atcttgggtt ttggttgtag accagtttta 540

ggttttgcac ctttcatttc tgttagttgt ttctgtgtgt ttagagaccc tgccacttgt 600

ttgacacttg ctacaatttc ttgatagtca gatagagtaa gatgttcagt caagtctgac 660

tcaatggt 668

<210> 107

<211> 196

<212> DNA

<213> S005(9)-350

<400> 107

tacgaagatc taaaactaag cattttgatg gattgagtgg tattgaacct ggtctttatt 60

atgatgattt cctgcatctt actacaccag aaatggcatc agaacaaatt gccgaattac 120

taacacttaa gtctaaatgt gtatttatcc ctcctagggc tgagttgagt gataagggta 180

aaccatttaa tccttt 196

<210> 108

<211> 692

<212> DNA

<213> S005(9)-355

<400> 108

aaaattatct tgtcacttga actcagcttt ccactttcat tgaaattgtg aaatgctctt 60

tgtggtggat atcgaaatgg ttccagtcca aaattaaatc tacattgttg aaaggacata 120

aaacttgctg ccgcaaaaaa tccttcctta acgttattga atatgtgcct ataaggatca 180

gtcgattctc catcgagagt aaaaataact tcttgtttgt ctaaatcgag taaacaacca 240

agaatcgagc cacttttcca tgttggcaaa ctgtgtggaa ttgacttggc attatgccat 300

ataatacgcc tacaaccatc aaatccaata gagtatttgt catcacctat accataacca 360

tcgtcactta gaaaactcga ttcttttgtt gcccaaccga tttgcatcac acccggtgtt 420

attaggagga cttcataata ccaacaacct ttggttatct ggaatgtgca gcggacactt 480

tcaaatgtgt aagcgtcaca gcgcgcttct aaaccatctg gtgatatttt caaatattca 540

cttacatcct tcgtattaag catggcgtta atattggaga cattggttgt ctcatatgaa 600

tattgtcgcc cgcttactgg aaagtagttg tctaaacacc aacaagcgca aaatcctatt 660

tgacgcatta agaaatttgg tgatgataca tg 692

<210> 109

<211> 569

<212> DNA

<213> S005(9)-357

<400> 109

catggctata tctggttgca ccgagggcat gatggaaaac tttggcgaag gtggatattt 60

tacacatggc aataagggct tattacctga tggcacttgg tggcgttttc ttttttctac 120

aataattaat acagctatgt tggtggtgtg tttgattggc gcggcactct ttgctaagac 180

atcggtatta attttgggca ttgtgtcaat atgtttgttg gccacatata taagttttct 240

ggctgaaggc ccaaaggaaa ttgatcgccc taaagaaaat accatctata atcagacgac 300

gaaacttcca tatactggtc tagacagtaa tacgttaatg ggcaatctgt atccacatta 360

tgtagaagat tacacctcga atggtaaaat ggttgatttc gccacaacat ttggcgtgtt 420

gttcgctggt gtgactggca tcatggctgg tgcaaatatg tcgggcgaat tgaagagtcc 480

atcgagaagt ataccaaccg gcaccttatc ggccgtttcc tttactttcg tttcttacat 540

tttattgtca ttcctgatgg catttacaa 569

<210> 110

<211> 530

<212> DNA

<213> S005(9)-358

<400> 110

tgtggtcggt ttttcccgca aagtaaactc aaaggagcag tttggtaacc gtttttctgc 60

tgttaaaacg gattttataa gagcatataa atcgtatttt cacatccata tatatgtgct 120

ttaaaataga ttcaatgaaa tgaaataaat acgctcactt tgttttccaa caagggtttt 180

tggggataat gcggttgtat atatgtatgt atatatgtgt cctcaggtat tcgggccgtt 240

gatgctaatt ccttctcctc cgcttccagt tggttgttca gtatgtatgt atgttgttcc 300

tgcttttttg tttattctag tttaatttaa attcgcgccc gttttcttgt ttttatttat 360

gtcttgcttt aacgtttcgc gcgcgatttt ctgtgtgttg ttctctgttt cttttacatt 420

actttttgct atttagatat gtgtggtttt gttttttttc ttttaggttt cgcgcccgaa 480

tagtgcttgt ttttaattta tgtcttttat ttttagtttg ggagcttccc 530

<210> 111

<211> 697

<212> DNA

<213> S005(9)-359

<400> 111

agccaatgat aggccctggc gcatccggaa ttgcaggctc gaagtggttt caccacaaat 60

tggagcaaat gtgcaagttg atatcctaaa cacagaaggg ttatcaatag caacatcagg 120

aatagcagtt gtaggatctt ctgcaagaaa gctatatgtc agaaatcccg acaaaacttt 180

cataggaagt ggtcgaactg acgctttcaa cctggtggca ataaagaacg tgtgcgcgaa 240

ctcagcgtta cagaccgttc aaatcaccgg aaatttgatg ttacaattag agttgcataa 300

cgaaatcatg caaactagtt gtggatacgt caaaacacaa gaggagattg actttaaatt 360

aagtcaaatg tctttatcat cacacactac cagtgatgaa cccgttattt tgcggaagga 420

ggatgcagaa agctagacac ctcaaaatgg acttagatat aagaattaat taagttcaaa 480

tggatgacca agttgatata cgcacccgaa taaaacgaaa tcgttgaccg aacatacaag 540

gaaacgaccg ttgaacaagg gaaaagctaa atgatacaac gtctacccat tagatatata 600

atgatagtat aagctagaac gagaccctta aaactcgagt aagctaaggt gaaggtcatc 660

aactttcact agggaaaaaa aaaaaaaaaa aaaaaaa 697

<210> 112

<211> 398

<212> DNA

<213> S005(9)-371

<400> 112

tattgccgtc tttgcgtcca atgctgccat tgcttgacct acatggtgtt cgccgtttgg 60

acttccacac atccctaatg gaggagctgc gagaaaaact aattgctcat attaatgaat 120

tgggacaaaa ggatccacgt gaacgtgata agaaattgag agaattgttg attaaaagtt 180

ttcctgttgt gcgagttaag gcgctccggc ctgtagtaat ggcaatttta cgtaataccc 240

aacatattga tgacaagtat ttgcgaatac tggtgcggga tcgcgaacct tattcggata 300

cggatacaga agtaaaacgg caaatttggc gtgataacca atcactgttt ggtgatgaag 360

tttcgccact tctgtcacaa tatattcgtg aaaaggag 398

<210> 113

<211> 727

<212> DNA

<213> S005(10)-388

<400> 113

ggatataaac gcaacacttc gtccactacc atactcagat atggtagatt attgatagtc 60

tcatatgaca ttttaccccg ttcagctaca agagcttcac aaatctcatt gcgcagacgt 120

tcttgtaaat cgggccgttt cgataattcg tatagagcaa atgtcatcgt cgatgatgac 180

gtctcaaaac cagctgacag aaatacggca gcttgtgctg tcaacatatc ctgtaactga 240

ggattatact cgcctttcgc aactgcctct tgtttaaggc tcaccaatac atcgatcaga 300

tcgttacgcg ttgctttgct ctcctctctt gacgtcataa catgttcgat ggtgccgcgt 360

aaaaaggaac tgaactctgt tgtaaaaagt ttaatacgga atgtagagac ccacttgggg 420

acgaaaaagg cgatgaagaa gtcaatggca cgacggagtg taaaggtgaa caattttcgt 480

ccttgagtgc gaaattcgcc gtttggattt acaagactgt tggcttttac accaaaagcg 540

atggtggcaa tcatgtcggt ggtgaagctg gcacaaacct ctttaacttc agttacgaaa 600

gcgccgttag tcttcgcata cgaattcaaa tgggcttcca actccgtgcc gatctcagtc 660

attagtggat acatttgttt gatctttccc gttgtgaaaa ccggcgttat tttcgatctc 720

aaatcct 727

<210> 114

<211> 837

<212> DNA

<213> S005(10)-390

<400> 114

ctcacaatct tgaattatac tttaataaga cattataaaa gagctaattt tataatgcag 60

aaagtcctac taatatataa aactaaaata atagataaag acataaccta agagcttatt 120

atgtcaaaat cttcgtcacg cttaattggt ttcaatcctt catatatacg ccaatctaca 180

tccacccaac tagggatgct tggacatata ccaaattgtc tccagtattg catacactta 240

ttacgaagca tttcataata ttctttacct tgaccatgtg catttaataa cattgattca 300

cacgaaatca atgatgaatt aagtagatca ttggatgcaa atgtccaatt acacgtttct 360

tctatggctc ttttatccat agcagcaaca tacacaggtc ttttttcgtg tcgcttaata 420

catcttttca aaaatgatgt ttctggagca aagactgtcg tatatagatt gatatcgcca 480

gttttcagcg catccgtaaa tttaatatta taacgatcaa agaagctaga tacattcggt 540

gcattaaacc aatgtaaaat atcctcttta acagtcataa ttaaatcatc tccgtaaaat 600

attgaacaaa cattattttt atatgattct aatgtcaacc caatattgtt ttcccttgat 660

aattgtaaat atgctgaacg gagatacatc atatttaccc cattattaag aatagttgta 720

atgggacaac ctgatggtgc accacaaaga acctgataaa ccatattaag cataagatgt 780

tgggcaaaag ctatttcatg tgccatagcc attctaacgc gcctatttgt tagatca 837

<210> 115

<211> 638

<212> DNA

<213> S005(10)-401

<400> 115

gagactacga gtaaatcgca aaggaattaa ttcttagtgg cattcttaac gagtgatcgc 60

gtgtcgccta cgtgttgtct atctggcgga aaggtggcgc tcattgcaga tcggttgaaa 120

gatataaaat tgccattaga gaaaacgttt atgtgttcac agtgaagatt tattgttaaa 180

gaaaataaag atttatttga agcatatcaa taattgtgga gtcttcttgc taaaatctgg 240

aaagggttaa aactgatttt taagaaataa gtgcttgctc caccaacatc aaaccatgaa 300

ctggctgtat ctactaacga tagcagcctt tttggctgtg agcagcgtga actcactatc 360

acttgatcca gtggcctcca ctgagctgga gcagcacata aagaagggtg attgtgtcat 420

atcaatgcga cacatacggc cgcgtcgcaa actacacata tccatagaag cactttttat 480

gatcgatttc cctacactaa aacataaatt atcctttttc ctcgatcgca tgaatcaacg 540

tgtcaccttg gatgtaacgg ccaacggtac cactgtatcg aaacacttcg aattgcccaa 600

tacgaatgag accagcacaa tacgttcgct ggcattgc 638

<210> 116

<211> 306

<212> DNA

<213> S007(3)-G11

<400> 116

tagccccaat tataactttc aaccaaactt tatagctcct caccgccgac agatttctct 60

tgatcggctt ttcttatgag ctcttccata tctttgcgca acgactcggt gccagcgcat 120

attaaatctg gtttcataat ttcaaatggt ccgccatagg gatgtgttac cacgccaccg 180

gcttcacgta tgagcaaata accggcagca caatcccatg ggtacctgct agcttatcct 240

tctcttcttg ttgcaacttg aagagcaatt tgactagagc ttcggcaact tcctcaggta 300

cctcgg 306

<210> 117

<211> 659

<212> DNA

<213> S007(6)-34

<400> 117

atggggaaaa cattgtaaac atgtaaagcg taactcttat ctacagcaat acgtttaaac 60

atttgattca tggattcctt cataggctta ggcagcatta gcccgtatat gcctgtgcta 120

agcgattccc ataaagcttt gatagcaggg aaagcctgct gagacccacg catgcgagca 180

ttcgtattag gatcagtagt taataacaca ttgcgcatag caacaacccc tgggttttga 240

tcccagatgg gaaaactaac ctgtcttcca gatgttgccg cttgaaacaa ttgctgtttt 300

tgatttgggc taacgccttt catattacaa tcccggtgtt aatttctgcc tttacgttgt 360

ttttcggcac gcccgccttg gacattgata gtagggccat tgctgaccaa gctgctacca 420

acaccagctt tttctgtagg tgcggccggt tcttctacca ctgctttttc tgggacttct 480

actacgagcg ctggctcaag gacttccggc ttggcttctt caagagcagg aacttgaacc 540

gcgacttctg atgtttcgtt cgccacggta gtaggcaatg caactactgg gctgatgctg 600

acttcatgcg ctgctttaaa gaattcacga aacaactctt tagcactacc ggctgaggt 659

<210> 118

<211> 490

<212> DNA

<213> S007(7)-107

<400> 118

ttgagctttc tctttattcc ttacgttaga ttttcccatt gataaagtat tgggtttaat 60

taatccataa aaacaaatta cttaactttg gaaagaaacc actgtatccg gtcaagcgcg 120

aaagcgcttg gctggatgcc aataaaattt tggattcgat tatgcttgaa caagctaagg 180

aatttaccaa tatgtccgaa ccaacccaaa ccaccagcat tactattcct gcaggttctc 240

cggctgctca ggctttcttt ggtcaaggta gctcccagac caactctacc tggatcatca 300

cggcgtttgt cgcgtttctg gtgatcgttt ggggtatctg gcgtggtcac tctcagtcca 360

gcgaagagca gaacactctg actcgtgcga tcgatgcaca ggcattccag tcaggtctgg 420

ctgcacaacg tttggctgat atgcaacaag ctatcactct ggaacgcgtt gcttctactc 480

agtacctcgg 490

<210> 119

<211> 483

<212> DNA

<213> S007(7)-178

<400> 119

atcacgcaaa cacggatgga tttgggatac aagaaaaatc tacggttatt ggttgcatgc 60

cccaatcgtc gagtgtgttt ttgcatggat tttctccgtc gcatttttca cgcaaaattg 120

ctgtaatatc cttttctgtg caatcatcaa taacacaatg cataaaaaca ccggaatact 180

gtcttattgc tttggtaatt cgaatggtat aatccaaggg gcagttaagt tcaaattcgc 240

tcgaactaaa gcgaataggt ctctgtaatc ttttctttct cttagaggtg ttaatacatc 300

tgcacagtaa tattccacat ctacgtccat agcctcggtg tctttaaaag aaaataggca 360

tgacttcttg ttgtcacaca acttttttat tccttcggtt ctgtcatatt ctacgcagtg 420

acccgtgatg catgctagcc caaccgtgca aagagcgagt gcccaaggga tggtacctcg 480

gcc 483

<210> 120

<211> 336

<212> DNA

<213> S007(8)-192

<400> 120

cagaagagag gtttcctcgg ccaagctggt ttggcaggaa ttaactacaa gtcaaattct 60

gccttatcgc gacaacaatt cgaacagtcg caaaagctgg ccgcgcaaca acaagagttc 120

actaaggaga accaacatcg tgaaatgctg ctaagtggcg ctagaacgga agtatctggt 180

gctactacgg cagtttcaca cgcctaatct aatgccgtga aagtaatgtg tgcggcatta 240

gggggcccta acaccaggac cccgtagcat ggacttgaga gagcactcca gtaagagccc 300

tgtcctgggc cagttcgcaa tataaagtac ctgccc 336

<210> 121

<211> 391

<212> DNA

<213> S007(8)-259

<400> 121

aaaaaggccg tgataaagga aaaccacgtt tgaacttcgt tcatatgcat aaagatcccc 60

tttgcaaaag tctcttgaaa taggatggtg acgaatgaac cctagcggcc ttgaagcatt 120

aatggctttc atgaacagtg atatagggca gctccttaag aatatcgttg ttaatgaagt 180

cgttcctttt gcaaaacaaa agttcattaa tggagcgatg aacccggtta ctatgactca 240

ggaagaagct gcacagcgtg cgactcaaga gtttgccact gtcgatattc ctgaagaagg 300

aataaatcca ggtaatgagc aggaagttcc tggtgagaat ctggataact ccacgtatta 360

gcggggaaaa aaaaaaaaaa aaaaaaaaaa a 391

<210> 122

<211> 427

<212> DNA

<213> S007(10)-420

<400> 122

ttttcgtcct acaggtcgat ctactatgtc ccatgttttg tttgaaattt gagccatata 60

ttcattttca acggcacgca gccacatatt acattcttca ctgtttatag cttcttgaaa 120

actagttgga tcattttcta ctgatatatt tgcatattcg cattcttcaa aatctacact 180

agattcagat tcagtatcgt ttttcttagt tttgacttct tgaaatgact tcttgggccg 240

tccgcgcgtt ccagttcgaa ttagctttgg tctacctttg ccccttttcg gaacgattaa 300

ttcagtattt atgtcttcat tttcactttc gcttgaagac ccctcaatta ttttctcaga 360

ttcaactatg ctttcatctt ttcgtgtatc gaaatctatc tccacagatc catcttcaca 420

gtacctc 427

<210> 123

<211> 748

<212> DNA

<213> S002(1)-A3

<400> 123

atgggggcat ttggtagtga aaattcaaag tgaacggttt atgtagaaat ttgagtgagc 60

attcgtttaa ccgatttaat aaagaaattg cataagttct catatttttc aatactttaa 120

ccgttatgga tggaaaaccg aagaaaacgc gccagcggaa tcggccaaga aataaagcaa 180

atcgtttaaa ggaggacaac ggccagacct cgaatagtga attaaataac actgagggcg 240

tagtgttaaa tattgtggga caacaaaaag cacttattaa cgaatgtgta aatgagaatt 300

acggagctct aacaaaaacc acgccgagtg gtagtgaatt gccaacgcaa tcaccgcaag 360

attcagacaa tggtttgaga ttactgcaac aaatgatgcg cacaaagctt ttacagcaag 420

gcaaaaagag tgaaaaaggc aatgttgata caaaagccac tgaaaaggtc gataaaaagg 480

gtcaaaaaag tagtgccagt ttgagcaaag gcaaagaaag tggtaaaacg acaaatggaa 540

aagcggcagc ggacaattta caaaagcaaa gtgcatttgt tgcaagtttg cagagaaaat 600

tagagagctc agtggcaaac ggcagcaccg atttacctaa tactttattg gaaaacctac 660

aaaaattact ccatgaggga ccaataagtg atgtagagtc ctcagacgac gaggaggatt 720

acatcgagta tatttataga ccgcggca 748

<210> 124

<211> 668

<212> DNA

<213> S002(1)-B4

<400> 124

atcacccaca tgcacatcgt aagcaacatg taacggaaag tcatgaggac gtcgagtcgc 60

accaccagcg acgccaccac gcacgaaact tccactcaag actcgcaact tcctcgcttg 120

acagcagcag caaccacaaa tgtgcagagc gcttggcaca gatttgcaat aatgtcggtg 180

ttagtgaaat tcagttggac gatgacaaag tgtcaccatt caaagtgctt tgcggtgccg 240

aacgttggat tgttgttctg cagcgtttag atggctcagt cgcctttcac aatcgcactt 300

gggatgcata taagcagggt ttcggtagtg taggtgaaaa aactgaattt ttcttgggtt 360

tacaacattt gcacgagcta acctatagtg gtttcttcga agtacctgcc cgggcggccg 420

ctcgaaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa 480

cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat 540

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 600

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 660

cgttgcgc 668

<210> 125

<211> 406

<212> DNA

<213> S002(3)-D9

<400> 125

aatttataca ccttatcaca tgcattggtg ccgccagcgt tgagacaacc atcaacaata 60

ttcagtaagc gttgttcacc cacaatttca tacagcgact ccttcatggc ctgtttgttg 120

atggcatcat ccttatagaa tactaattta ttgtagagac agttcacgta gcacttctcg 180

cgatcacttg ggttcgccac ctttatttta accagattgg tggcgagctc atccgaaaaa 240

ccatattctc ccacacaagc ttccttggcg tgtttcaaat tgagacgata ggcaatccaa 300

gcggtcaaaa cttcgaactt cgcttccaat tccggtgtga gcttcttggg cttgcgcaga 360

cccgcttcgg cgttgaaaac gcacagactg agcagtacct cggccg 406

<210> 126

<211> 624

<212> DNA

<213> S002(3)-E7

<400> 126

tcaccaagtt tggccaaact ttgacggaat tctttaactt tagctggatc tgcctttgct 60

tttgctccac tagcatcaag accttttcct tcctcttcaa tgtaaaatct caaattctcc 120

aacaaaataa tagtgccagg tgctggatct ttacattcat tttccacttc gggtccaacg 180

caatcaggta aaaatttcac atcttttcca agaagttttt tcagttcatc agccactgga 240

gcaagggtat acttttgatt tttttgtcca tctggccgac ctaaatgcga catgagcacg 300

acagatttcg cttttgcgtc caacgcaaat ttgacactat ccaaagcggc aacaatacgt 360

tgattgctag taattttgcc atctttgatt ggaacattga agtccactct catcaacact 420

cgtttgccat ttaaatccaa actttcgatg ctcaacttgt taaaagccat tttaccggtt 480

ttactctttt cttttttatt acaagaggat agacttttct gattagtatt tccgaaataa 540

gattggaatt gaaaagtttt ccgcacgcac aaaatgttcc ttctgcttat cggtatggta 600

aaactttttc cccatgtacc tcgg 624

<210> 127

<211> 410

<212> DNA

<213> S002(4)-C10

<400> 127

gccagcagtg aataacaagt gaccagtttg ctgaaactgg aaaatacgcg cttgtagaga 60

gcaacagact atcttttaga agactgacca gcggtatcgc gagagtgtta agacaacaag 120

aacaatgtgc gcaatgcagg ggattttact tgccttattg atcgctactg cggccgcaca 180

atcgcaatcc tattaccaac agcaacaaca agcacaatat gagaaacaat atgaggcaca 240

agaagctgag tatgcagcac gcagagagca gcaaagcaca cccgcttact tgacaaggat 300

atcattgacg acaacctcct ataccaacgg gattttattg ccgcatttta acataagacc 360

aacaacatca cctactgtcc agcacccagc agggatcaat gtacctcggc 410

<210> 128

<211> 631

<212> DNA

<213> S002(4)-E4

<400> 128

tatatgaaac aaaatttcag tttggaatcg gatgattcag tagatattcc atatcatgcc 60

agagaaactg cactgccatt cacatatggc gacatatctg aaggattaac gcaaagtcaa 120

ctgaagcacg ttgagagtat ttttgacgaa tcacaatttg aaaatgataa acaaccgttt 180

ttaaaggaga cgaactcttt atataattta ggggagacag caatgattca agatgtatat 240

ttaaaaaaga aaattgccaa caatgaaaag cttcaacaaa gctatgacaa ttcaattaat 300

aaaaacaatc agacagggaa acgtgagctt ctctcaaaga acaaactaat caataatata 360

gtgcgaagtg aaaacaatac tcgtaagtta tccgaaaatg tccataatag gataattttg 420

gaacaagatg aaattagtaa tgagttatta gatacatcac ataacggttt aaatcttaat 480

agagagaatt caaaagatca acctatccta gaaactaagg atctaaaaag gtgtcaagca 540

catagtttcc catatgagga aatcacaagc tttttaaatc gttcgactag cccctatcca 600

acgctcagcg cacaaatttg tagtacctcg g 631

<210> 129

<211> 408

<212> DNA

<213> S002(4)-F8

<400> 129

catggaattt atacacctta tcacatgcat tggtgccgcc agcgttgaga caaccatcaa 60

caatattcag caagcgttgt tcacccacaa tttcatacag cgactccttc atggcctgtt 120

tgttgatggc atcatcctta tagaatacta atttattgta gagacagttc acgtagcact 180

tctcgcgatc acttgggttc gccaccttta ttttaaccag attggtggcg agctcatccg 240

aaaaaccata ttctcccaca caagcttcct tggcgtgttt caaattgaga cgataggcaa 300

tccaagcggt caaaacttcg aacttcgctt ccaattccgg tgtgagcttc ttgggcttgc 360

gcagacctgc ttcggcgttg aaaacgcaca gactgagcag tacctcgg 408

<210> 130

<211> 339

<212> DNA

<213> S002(5)-E5

<400> 130

aattctcttt cagtagaaga agttggtgcg cctgacgcaa gctcggacga gacactagaa 60

gacacaaagt tacttgaaaa gcgttccgct gacgccatac ccgacgtaga cttgtgcaaa 120

caccacaagc acaataaaga cagtaatgaa tttggtagtg aagatgctta cgagcgttat 180

tatcaactcg aaaaatcata taatagtgaa cttcgaagga agcgtcagaa agttgacaag 240

aaagactcat cacaagagga ttcctccgag ctggcaggca gtgatttgct agcactagga 300

ttacgtagaa aacgcaaagc agccgatagt acctcggcc 339

<210> 131

<211> 515

<212> DNA

<213> S002(6)-16

<400> 131

catgaaattt atacacctta tcacatgcat tggtgccgcc agcgtttaga caaccatcaa 60

caatattcaa taagcgttgt tcacccacaa tttcatacaa cgactccttc atggcctgtt 120

tgttgatggc atcatcctta tagaatacca atttattata gagacagttc acgtagcact 180

tctcacgatc acttgggttc gccaccttta tttttaccag atttgtggcg agctcatcag 240

aaaaaccata ttcgcccacg caggcttcct tggcgtgttt caagttgaga cgataggcaa 300

tccaagcggt caaaacttcg aacttcgctt ccagttctgg tgtaagtttc ttgggcttgc 360

gcagacccgc ttcggcgttg aaaacgcaca ggctgagcac tactgtcgcc agtatgcagc 420

agacgataga ggatttcatg tttagttgat tttgtgggtt gattttagta gattactgta 480

atgtataatc gaactgaatc cccatgtacc tcggc 515

<210> 132

<211> 475

<212> DNA

<213> S002(6)-19

<400> 132

ttatatacat tatcacatgc attggtgccg ccagcgttga ggcaaccatc aacaatattc 60

agtaagcgtt gttcacccac aatttcataa agcgactcct tcatggcctg tttgttgatg 120

gcatcatcct tatagaatac caatttatta tagagacagt tcacgtagca cttctcacga 180

tcacttgggt tcgccacctt tatttttacc agatttgtgg cgagctcatc cgaaaaacca 240

tattctccca cacaagcttc cttggcgttt tcaaattgag acgataggca atccaagcgg 300

tcaaaacctc gaacttcgct tccagttctg gtgtgagctt cttgggcttg cgtaagcccg 360

cgtccgcgct gaaaacgcac aggttgagca ctactgtagc caataagtag cagacgatag 420

aggatttcac gtttagttga ttttgtgggt tgattttagt agattactgt acctc 475

<210> 133

<211> 596

<212> DNA

<213> S002(6)-82

<400> 133

atccaataac ttagttagca tatcttgtat atacaattta tatccttctg aagtttctaa 60

tgttttttga atatcacgga tttcattttc cttatgatga agctcatata aggcagaatc 120

acaacggtta cgactttccc gtaaatcctt ctgagttgca attaattctg ctcgtgtcct 180

actaagttct tccgaaataa gcgtttttgc ctggatctca gcctgaagtg aactttgtaa 240

atttaataac tccatcttat cgagtttttg agaacgtcta tttcgccaat ttttcgtttc 300

ataggtttgc tgaaatgaac ctgagtgttt tagatattca acctcctcag tcatttttgt 360

tgctaaggct tgtaagtaac ttcgtgcatc cttttcatca gatacccatt gtataatttc 420

ggacatttgt cgttcccatt gtattatagc ttgtcgcttt gttcttaatt cttcacaatt 480

attttggtct tctatttgct ttgtttgtaa ttgtcttata gattctttga gattttccat 540

ttctgatatt aatcttcttt tttcttccac ccatatactc ttctcttgct cataca 596

<210> 134

<211> 186

<212> DNA

<213> S002(8)-270

<400> 134

tcccaagttc tttaaaaaca gcaaacgaat gaagaacgaa ttacaaataa taccacactt 60

ccagcaaaag ttacacgcag cgtttaatga ccaaagtgcc gacgacgcct aagtatatga 120

ccgctaaaga agatagacac aacgcgaaag ttctctcact gtgttcgtcc ccatgtacct 180

cggccg 186

<210> 135

<211> 425

<212> DNA

<213> S002(10)-416

<400> 135

tgctcagtct gtgcgttttc aacgccgaag cgggtctgcg caagcccaag aagctcacac 60

cggaattgga agcgaagttc gaagttttga ccgcttggat tgcctatcgt ctcaatttga 120

aacacgccaa ggaagcttgt gtgggagaat atggtttttc ggatgagctc gccaccaatc 180

tggttaaaat aaaggtggcg aacccaagtg atcgcgagaa gtgctacgtg aactgtctct 240

acaataaatt agtattctat aaggatgatg ccatcaacaa acaggccatg aaggagtcgc 300

tgtatgaaat tgtgggtgaa caacgcttac tgaatattgt tgatggttgt ctcaacgctg 360

gcggcaccaa tgcatgtgat aaggtgtata aattccatgc ttgtgcctca ccggaattcg 420

acaaa 425

<210> 136

<211> 401

<212> DNA

<213> S002(10)-452

<400> 136

gcttacgcaa gcccaagaag ctcacaccag aactggaagc gaagttcgag gttttgaccg 60

cttggattgc ctatcgtctc aatttgaaac acgccaagga agcttgtgtg ggagaatatg 120

gtttttcgga tgagctcgcc acaaatctgg taaaaataaa ggtggcgaac ccaagtgatc 180

gtgagaagtg ctacgtgaac tgtctctata ataaattggt attctataag gatgatgcca 240

tcaacaaaca ggccatgaag gagtcgcttt atgaaattgt gggtgaacaa cgcttactga 300

atattgttga tggttgcctc aacgctggcg gcaccaatgc atgtgataat gtatataagt 360

tccatgcttg tgcctcaccg gaattcgaca aagtacctcg g 401

<210> 137

<211> 590

<212> DNA

<213> S002(10)-444

<400> 137

gtgaagttga agagaagaaa cagcgcgaaa tcgaagagaa gcgtcagcgt ctcgaggagg 60

ccgagaagaa gcgtcaagct atgctgcagg ccatgaagga caaggacaag aagggaccta 120

atttcactat tgccaaaaag gaggcaggcg tgttgggact ctcatccgct gccatggagc 180

gtaacaagac taaggaacaa ttggaggagg agaagaaaat ctctttgtcg ttccgcatca 240

aacctctggc cattgaaggt ttcaacgaaa gcaaattgcg tgagaaggcc caagaactgt 300

gggaactcat cgtcaaattg gaaactgaga agtatggctt ggaagaaagg cagaaacgtc 360

aggattacga tttgaaagaa ttgaaggaaa gacagaagca acagctcagg cacaaagcct 420

tgaagaaggg tctcgaccct gaggcttcga ctggcaaata cccgcccaag attcaagtcg 480

cctccaaata tgagagacgt gtagacacac gctcatatga cgacaagaag aagctcttcg 540

aaggcggctg ggaagaaatc ggcaaggagg tgaatgagaa gatctggaac 590

<210> 138

<211> 293

<212> DNA

<213> S008(1)-B2

<400> 138

tacgcccaat accgataccg aaatctttct ccgccgccaa gttgaacacg accagcataa 60

gtatacgtgc accaaatata acttcaacaa ttcaaccggt cggtagtagt ggtgtggttg 120

gcagcactat taattctgtg gtggggtcag gtaatacaac tgctaacaat tcgccacgca 180

atacgactat gagtggcgta actacaggtg ttggcaacaa cgtcataccg acgtcgaatt 240

tacccaccac acgtatcata cagctgcaac aacagcccgg cagtacctcg gcc 293

<210> 139

<211> 186

<212> DNA

<213> S008(1)-D11

<400> 139

agcgaggaat cttctaacag caaacagacc aaacctagtg gtaatttggt ggttattgat 60

ttgggcgccg tcatacgcaa tttcgaggaa cttgttctgc tcgatatcaa ccgcgtcggt 120

gctgcgatcg gtaacacttt ggttcaactt acgtcgcaaa ctgatgtacc tcggccgcga 180

ccacgc 186

<210> 140

<211> 343

<212> DNA

<213> S008(1)-D5

<400> 140

ggttaaggct gctcttcgga gcacaagcaa tcgagcaaac tcaaatcgtg aaaaatctat 60

tgaatacaaa ttggttccac aaaaataaaa acgcaaaaca aactaagcag gaaaataaaa 120

gaaaacttgg aaaaacaatt cgcttcatat cataacctct ctggtcgccg tctgttaacg 180

agcgctgctt gctattgatc gttacaacag catcaccttg acttcggttc gcctttactc 240

tgcttaatcc cgccactttg taaacattca cattggcgcc gccgacaaat ctcaattata 300

cgatggctgt catcagtgat catggcagca gtacctcggc cgc 343

<210> 141

<211> 718

<212> DNA

<213> S008(1)-D6

<400> 141

ttatggcttt cgtattagcc attgctaatg cttcacccaa gcatggcaag aacaaggata 60

acatgaatag cttaaaacct gtggattggt tatcggcctc tgaattagaa tcgatgccat 120

cagtcgagga tatcactttg caacagttgg agaacatgtc agtagaagat gcccagcgaa 180

agattgagaa gctgtatcac ttgtcacaaa ttaatcacgc tctagaaccg tcctttgttc 240

cgagcccaag taatgtgcca gttatcttga tgaaacccaa tggacagccc gagcgcacca 300

atctcaatga actcgtccaa actgccaaac aacaacccaa ctttggcgat gaggaagtca 360

ccattttcat tactggtttg ccacagtcgt ccccttctgt tgcaaaggca aacaaaaaat 420

tgatccaggc ttatatgcag cgctacaatg gacagcgtca accaatcagc aataaccagg 480

actatgatta tggcaacaat aaggataatc aaggtgccac ttcaagtgaa gaagactaca 540

gtgaatcgtg gaagaacccc aaacccacaa agggcaacct tgtggtcatc agcttgggtt 600

ccaccctcac caatatgaaa cgtttggctc ttatcgatgt agaacaaacc ggtaacatga 660

tcggtaaagc tctcgtcgaa ctgaccaacg aatgtgatgt acctcggccg gaaccacg 718

<210> 142

<211> 186

<212> DNA

<213> S008(1)-E3

<400> 142

atactgtggg gagcgtttca ctaaagcgac atttccagct tctgcggtag gcagtaacat 60

ccccaatacc gctattagaa gaggcatcgt aatgcagatc aaaagtattt tctttgttgc 120

tgccattggt gctaaaattc gatatgaaac aaatattttc tcccatgtac ctcggccgcg 180

accacg 186

<210> 143

<211> 272

<212> DNA

<213> S008(1)-F10

<400> 143

tagacgtctg aaccgccctg agaattgcgg cgtcgacatt cccggcacct cggtattgca 60

tggtcagcta caacattgtc attggagatt gctcttgtag cactttccga attgtgttga 120

agtttaaagc aaggtaaatg ctgaagaaat gttgcacccg atgcgcgtaa ctcttgatcg 180

cgaacagctt cttcggctgt gagttcttct ggtggggtat gattttcatg ttcgcccatg 240

ttaaagtgat tactattatc attgagtacc tc 272

<210> 144

<211> 569

<212> DNA

<213> S008(1)-F12

<400> 144

taccggtttg ttctacattg ataagagcca aacgtttcat gttggtgagg gtggagccca 60

agctgatgac cacaaggttg ccctttgtgg gtttggggtt cttccacgat tcactgtagt 120

cttcttcact tgaagtggca ccttgattgt ccttattgtt gccataatca tagtcttggt 180

tattgctgat tggttgacgc tgtccattgt agcgctgcat ataagcctgg atcaattttt 240

ttgtttgcct ttgcaacaga aggggacgac tgtggcaaac cggtaatgaa aatggtgact 300

tcctcatcgc caaagttggg ttgttgtttg gcagtttgga cgaatccatt gagattggtg 360

cgctcgggct gtccattggg tttcatcaag ataactggca cattacttgg gctcggaaca 420

aaggacggtt ctagagcgtg attaatttgt gacaagtgat acagcttctc aatctttcgc 480

tgggcatctt ctactgacat gttctccaac tgttgcaaag tgatatcctc gactgatggc 540

atcgattcta attcagaggc cgatgacca 569

<210> 145

<211> 289

<212> DNA

<213> S008(2)-B1

<400> 145

gcttttcgca tgcgaaagag cgaagatgaa aatttgctta agaaattgac gtcacgtgag 60

cgccgcttca tcaagttcgc atcggtggaa taccgcggcc aattgtatat gacaccgcaa 120

gatttcctag attctgtggt tgagcaagag ccaagacctc gcctgaaacg acgcatgctg 180

agtgaccaag acttgcagac cataaaagac aacacaccgc ccttgaagaa aggctcaaaa 240

atgctatttc gcacactaag ggacagaggc attgtttcgt acctcggcc 289

<210> 146

<211> 194

<212> DNA

<213> S008(2)-F10

<400> 146

gcttccgcgc aaatcgcaac gataagtgcg aatagaatgc aaaagcgcat tttgtcgaag 60

atatgtattt agtttttttt ttgatgacac gacacttgtg ttctgttcgg taagaagtcc 120

gaatttctac tgcatttctt ttacaggact gaccagcgct tttatactgc cccatgtacc 180

tcggccgcga ccac 194

<210> 147

<211> 219

<212> DNA

<213> S008(2)-F12

<400> 147

ggcaactaac aacacactac atacgctcag atagcggact gcgcacaaca ttcagataaa 60

taccgccttc ggcctgtatc actaccgcct tgcgatccag ctgcatggga atcttggtgc 120

gagcacaagt cgtcactttg tggttaagta gaaaatagtg cgtataatgt tgccgatagc 180

tgctacttac ctcttggcgg ctctcttggt acctcggcc 219

<210> 148

<211> 519

<212> DNA

<213> S008(2)-G1

<400> 148

acatgagtcc ttcccacctt gaggcacacc agcacatagc atgcgttccg ttatgacacc 60

ataaggcgca tactcagttg cacatacaat gggatccacc acattcacag tgactgcttg 120

caatgtgggg gataatgatc caccctcact ggtgctgccc caaccggtga caacagcagc 180

tgtgccatcc gccggactta tgctggacat acgtatcggt tgcgtcgagc tactgaacgg 240

tatttgattt cgtagcttaa tcaaggcaat gtcatagtcg gaggtgagtg ggttgtagtt 300

ctcatgtcgt accagtcgaa ctacaagctc cacggttggt tgggaaagct gtgaacgctt 360

cactagagcc acttctcgag cttcagcccc cggcaacaac atgctaaatg ccgcaattaa 420

aagaggcaac gcgacacaaa ttatgaaaat tttcttcagt gtggccattc ttagtggaga 480

tagaagtgtg aaaataaaac tgtgccccca tgtacctcg 519

<210> 149

<211> 558

<212> DNA

<213> S008(3)-B10

<400> 149

aatgggtgag gaaccaacaa cagtccgact aatgaggaac aaatataccg gtgagccggc 60

tggttactgc tttgttaatt ttgcgactga cgaaagtgca atggatgcta tgcataaact 120

aaacggcaaa cccattcctg gaactaatcc gattgttcgt tttcgattaa atagcgcaag 180

taattcatat aaacttcctg gaaacgagcg agaattcagt gtatgggttg gagatttaag 240

ctcagatgtc gatgattatc aactttataa ggtgttttca acaaaatata cttctataaa 300

aactgccaaa gtaattctgg atagttctgg gttttcgaaa ggctacgggt tcgtacatgg 360

gggaaaacgt gcaaggaata gcagacattt tgatttaatt ttttttgttt ttcgtatcgg 420

tatttagttg ctaaattcat atgtaatttt tcgcgttttg aaattacgta tattttgttt 480

ttacattaag tactctgcgt tgataccact gcttacatgg gacccgagga ggttagggaa 540

atgcccgaag aggtgcgt 558

<210> 150

<211> 243

<212> DNA

<213> S008(3)-C11

<400> 150

agtgaaaatt aacaaattta tctttaattg ggcgtattgt gttaattgat tttactaaat 60

aaataagttc tttattagaa atgattggtg gattatttgt atataaccac aagggagagg 120

tattaattag ccgtgtgtat cgagatgata ttggtcgtaa tgcagttgat gcttttcgtg 180

tcaacgtcat ccatgcaagg cagcaagttc gttccccggt aacaaatatc gcacgtacct 240

cgg 243

<210> 151

<211> 470

<212> DNA

<213> S008(3)-D10

<400> 151

aacgagccaa acgttggcat tctccaagat ggattggtaa gatggggtgt tcttgccgat 60

cttcaagacg caacgccact tggcgaagtc gcaaccgtcc ttcttgtatt gggcgcaacg 120

ggcagccaaa tcatccaaac cctgagtggt gcactcgtct tcggagccga acaatggcac 180

gacaccctta tcgaccttaa tgccggggat gatgccgcgc ttcttcaaca gctcaacgaa 240

tggggtgtca tcatcagcct tctggtacgc atgcccacat gcgccctgcc atacatccac 300

tctattttgc aggagcatat aggaagtatt gttcaatttg gaccaaaata caatgaactg 360

agttgtgctg gtttgaaagg tttcgaaaca tttattaatt aaactgcaag tgttggaaaa 420

ggcaaaaggc cacagagaga gcagagcatt aaagcgaaaa gtacctcggc 470

<210> 152

<211> 405

<212> DNA

<213> S008(5)-B12

<400> 152

ggtttagtcg tttcttttga actatcccca tgtacgctcg ggctgtccat tgggtttcat 60

caagataact ggcacattac ttgggctcgg aacaaaggac ggttctagag cgtgattaat 120

ttgtgacaag tgatacagct tctcaatctt tcgctaggca tcttctactg acatgttctc 180

caactgttgc aaagtgatat cctcgactga tggcatcgat tctaattcag aggccgataa 240

ccaatccaca ggttttaagc tattcatgtt gtccttgttc ttgccatgct tgggtgaagc 300

attagcaatg gctaatacga aagccataaa gcagaaaatc tttagaggat tcatactgat 360

cacgtttgca aatcggtttg aactgtcccc atgtacctcg gccgc 405

<210> 153

<211> 355

<212> DNA

<213> S008(5)-B8

<400> 153

tacatgtttt ttgccttcaa aatcgatacg atgcagattc tgtattaaaa gcaacaataa 60

attactatta tacagttcct gcgacagctg ggcgactaca taatccgctg gtggctccga 120

atcactgcta ccgtaaagca tatttttaat ggagacaaga tttttgctga catcttcttg 180

tgctttttct gctttacgat caccacccgt ttctagtgca ttgattgctt ccttaagcga 240

ccgcaccaat tcagcggggg tcttttgtga ttttccaaac aagggcatta cgttggcact 300

ttactttatt cacacccggt ttaccgcaca cctatttttt cgtacctcgg ccgcg 355

<210> 154

<211> 170

<212> DNA

<213> S008(5)-C7

<400> 154

gttatcacgt attgagttgc ctctagcgca cactgttgtt gctgtagcca acacggccac 60

caaacaaaaa atacttaaag gactcatggc tcttcgttac cgtttatgtc gtatgccggg 120

agcagtcgaa ttgaactgtg gtccccatgt acctcggccg cgaccacaaa 170

<210> 155

<211> 321

<212> DNA

<213> S008(5)-E5

<400> 155

tttgtcagaa gctgatgatc tctatactta ctacaataac tctgtaaaaa atctgaagtc 60

acgtgataca gaagtggtaa gtaagaaagt ggatgacttt gttgagatcc tcaaaatact 120

gaaacgcaaa gttgattctt ccgatattag attgtcacct gaagacttca ccacagttga 180

actggtagca ggtcgtatta atgagctggt ctctatagcc ggtcgtttaa tagcaatgct 240

gaatgatctt gccagacatc tcgagttgca gattcaggca ttccgcgatt acgcaaaaaa 300

aaaaaaaaaa aaaaaaaaaa a 321

<210> 156

<211> 399

<212> DNA

<213> S008(5)-G11

<400> 156

attgtgcaat cagctgcatt cagagatggc ggtcgatttc cggtgctcag ctatcagcat 60

gaaaatgggg ctacgctaat gcgcagctca cagcccgtct ccatacaagg tattaaacgt 120

tgtcgtgctg acgaagcgat actgaacgtt gtgctgggac gcagtaaaaa ggggtttatc 180

gttgacactt ggggaaaggg taaatcaaac accgaaaccg atcaacatta ctcacagtgg 240

aagaaagtga atcgggctat tggcaatgtg tcggcgcctt cagcaatttt agatagttac 300

accaaactta ttgaagcatg caatgatatt acatgttccg cggacaaatg gttgtcacgc 360

ctagaagcaa gcggctggtt aagtctcgta cctcggccg 399

<210> 157

<211> 427

<212> DNA

<213> S008(5)-H2

<400> 157

aatcttcaga gccaatcctt atcccgaagt tacggatcta atttgccgac ttcccttacc 60

tacattattc tatcgactag agactcttca ccttggagac cagctgcgga tattggtacg 120

gcctgttgag aagtttgcgt aaccccacca taaattttca aggtccgagg agaaaatatc 180

gacacaacag taaatgtcat gctcttctag tccatctacc atatctctct tcgaaagact 240

tccatggtag tacgactata aaacagaaaa gaaaactctt ccgatacctc tcgacggctt 300

ctttatggtc gttcctgttg ccaggatgag cacaaggccc atttttaata acaaacggat 360

actcaacagg ttacggaatt ggaaccgtat tccctttcgt tcaaaattat tccccatgta 420

cctcggc 427

<210> 158

<211> 480

<212> DNA

<213> S008(6)-14

<400> 158

tttgcgattg ggcgaaaccg atgaggcggc cctgaaagat cgtgtagatg aagacgcgga 60

tgattggcgt ctgtcgcgac agttgcgcgc tatgaaatcg aaggcggttt tggataaact 120

cacctttttg cgcggttgtg atgagaagga tgtggaggag ctcgtgccgg gcaagctttg 180

cactttctgc aatatcggct ttcaaaacac cgaaacactg tggactcaca tggtgagcgc 240

gcatttacca ccggcacgta aactgcaacg gaacagttat ataacgaccg atactttgca 300

ctcggaatcc acaatggata cagtctgttc gagtggtaga cgtagctact tatcacacga 360

tacggaaacg gaatcacaaa ccgtttccga gccgtctaac ttgagacgca gtcgtcgtcg 420

cggcattttt cggaatagac aacgcgtgcg tccaaaaaac caggtggtac ctcggccgcg 480

<210> 159

<211> 482

<212> DNA

<213> S008(6)-29

<400> 159

tagcactatg cgtatgctgg ccaacgacag taaccacatg tcgcaggagt gccacaaaac 60

acctcccaat gacgtgctgc atactttgaa cagcaacgca gttggcaatg ccgcaggtgg 120

cgtagctgtt aactgcaatc atacggaagc tgtcaatgct gatatggacg gggaaatggc 180

caacaatgtg tcggacggct ttgctgcagc tggtggtctt ttctgctttc ggaatgtaat 240

catacaagat aatcactttc agcggacacg tcaggtatat gtgaatgagg gcaatgtaaa 300

attgcaattt caagacttta accaagaagg tgaccccatc acgcagatct ggcagaaata 360

tttggaaatg caaaaatatg cgctacaaaa gccgaccgcg cctcatagtg caacgcaagt 420

ggcaaatgct gtgcctattg ttgtatccac cgaagagcta caaacgatgc aaggtacctc 480

gg 482

<210> 160

<211> 322

<212> DNA

<213> S008(6)-42

<400> 160

gacaggtgtt ggtgcacaag ttgccggtgc tgctggacgt caatacaaac gttcgacagg 60

ccatcaattg cgtcgtatca cagctttgga ccctgcaaaa ttgttcgcta aggacaagga 120

tatgttaact ggtttggctc gtggtgatgc tgatttcgtt gatgccattc atacttcgac 180

ttgtggaatg ggaacacgcc aacgagttgg tgatgtcgac ttctatgtca acggtccagc 240

ttccgctgct ccaggcgcta ccaatgtaat tgaagcaact atgcgtgcaa ctcgttactt 300

cgccgaatct gtacctcggc cc 322

<210> 161

<211> 167

<212> DNA

<213> S008(6)-58

<400> 161

atcgtcctac cagcagttac agcggaagat tcagccgaac attcagtttt agtgaagagg 60

ggtatcaatc ttgctaaagt ggtgaaagta gtcaaaccag tagtaaaggc actggcacct 120

ttattagcaa acatctaaat ccgaaacaac cgaagcgtac ctcggcc 167

<210> 162

<211> 216

<212> DNA

<213> S008(6)-64

<400> 162

aagagaaggt cggcggctgg tggaaggagg gttgcgccaa gagtaagtta aacggtatgt 60

atgcgcctac aaaatgggag gaaaaacagc aagatggtat tatatgggat aataagaccg 120

ttgtgggcat gaattactcg ctgtcgtatg tgcatatggc gatacgacca aaatattgct 180

acaaaattaa aacggaattg tgaaatagta cctcgg 216

<210> 163

<211> 339

<212> DNA

<213> S008(6)-77

<400> 163

atcaagataa ctggcacatt acttgggctc ggaacaaagg acggttctag agcgtgatta 60

atttgtgaca agtgatacag cttctcaatc tttcgctggg catcttctac tgacatgttc 120

tccaactgtt gcaaagtgat atcctcgact gatggcatcg attctaattc agaggccgat 180

aaccaatcca caggttttaa gctattcatg ttgtccttgt tcttgccatg cttgggtgaa 240

gcattggcaa tggctaatac gaaagccata aagcagaaaa tctttagagg attcatactg 300

atcacgtttg caaatcggtt tgaactgtcc ccatgtacc 339

<210> 164

<211> 387

<212> DNA

<213> S008(6)-85

<400> 164

agagctttac cgatcatgtt accggtttgt tctacatcaa taagagccaa acgtttcatg 60

ttggtgaggg tggagcccaa gctgatgacc acaaggttgc cctttgtggg tttggggttc 120

ttccacgatt cactgtagtc ttcttcactt gaagtggcgc cttgattgtc cttattgttg 180

ccataatcat agtcctggtt attgctgatt ggttgacgct gtccattgta gcgctgcata 240

taagcctgga tcaatttttt gtttgccttt gcaacagaag gggacgactg tggcaaacca 300

gtaatgaaaa tggtgacttc ctcatcgcca aagttgggtt gttgtttggc agtttggacg 360

agttcattga gattggtacc tcggccg 387

<210> 165

<211> 584

<212> DNA

<213> S008(6)-93

<400> 165

ttaacaaatc aaaaatatca aaatgaaatt cgtcatcgcc gctgtctgca ctttggctct 60

cgccatgagt gctcaatcct caatcattcc cggtctgctg cccggtctta cacaaatcgc 120

cggacacagt ctctcctata ccgccgtctc tggaccattg gccgttcccg ttcccatttc 180

cgtcgctcac gctccagctg ctgtaatcgc tgccgcctca ccaccaattg ccattgtttc 240

tcatgctgct gctattgccg caccctctgt cgctgtgcat gcacccgctg tggcggccgt 300

tgctcatggc ggtgtcgtag ccggtggtgc ttatgttgcc aagacccgtg gtgctgtgca 360

ttcggctcca ttgcccggac acatcgcctc agtggccaat gtgaatgttg ctcccgcacc 420

aggcacctgg taaattcagc caatctagct gcacatccgt ctaaatcaat tcttccccca 480

atggtgacat ggctaactct ttaaggacct gcaagaaaat tataaatgtg tggctgaggc 540

gcacattagt cgataaagtt aagtaggcgc aaatttgtac ctcg 584

<210> 166

<211> 646

<212> DNA

<213> S008(7)-130

<400> 166

gtcgcacaag gtattggcgc tcatgtggcc ggtgctgctg ctcgccaata cacacgtcaa 60

acaggaaaca agttgcgtcg tatcactgcc atggatccct caaaaatctt tgcacgcaaa 120

cccaacactt tggtcggctt ggctcgcggc aatgctgatt tcgttgatgc catccacact 180

tctgcttatg gcttgggtag cgctgctcgt gccggtgatg ttgacttcta tcccaacggt 240

ccttctgttg ccatgcccgg cactgataac attatcgaag cttccttacg tgcaacacgt 300

tacttcgccg agacggtgcg tccaggtaat gatcgcaact tcccagctgt cgcagccgaa 360

tccttacaac agtataaaaa caacaatggc aatggcagac gcgcttatat gggtattgcc 420

gctgactacg atttggaagg tgactacatt ctgcaagtga acgctaaaag tccattcgga 480

aagagcgcac ccgccaagaa acaaaattcc taccatggca tacatcagac ctccggccgc 540

tcctcttcga acaactaaag tgcagttgaa ggcggaaaag taaatagcag agctttaaat 600

atatttcgta tttaatttcc cttgcaaaaa aaaaaaaaaa aaaaaa 646

<210> 167

<211> 550

<212> DNA

<213> S008(7)-135

<400> 167

cacactcagt tctggcgtta tttcgcaggt aacttggcct ccggtggtgc tgctggtgcc 60

acttctttgt gcttcgtcta ccccttggac cttgcccgta ccaatctcaa tgaactcgtc 120

caaactgcca aacaacaacc caactttggc gatgaggaag tcaccatttt cattactggt 180

ttgccacagt cgtccccttc tgttgcaaag gcaaacaaaa aattgatcca ggcttatatg 240

cagcgctaca atggacagcg tcaaccaatc agcaataacc aggactatga ttatggcaac 300

aataaggaca atcaaggcgc cacttcaagt gaagaagact acagtgaatc gtggaagaac 360

cccaaaccca caaagggcaa ccttgtggtc atcagcttgg gctccaccct caccaacatg 420

aaacgtttgg ctcttattga tgtagaacaa accggtaaca tgatcggtaa agctctcgtc 480

gaactgacca acgaatgtga tgtacttgac cgtttggact aattttccgt tctccccatg 540

tacctcggcc 550

<210> 168

<211> 721

<212> DNA

<213> S008(8)-276

<400> 168

gttggtgaac tacaaacatt tacctctcgc actaccaata aggaattcaa aaaacgcgaa 60

ctgacactgg ttgactccag caatgctgct gtgaatttaa cattatgggg cgaagatgct 120

gttaacttcg atggctatgt gcaaccagtg attttagtaa aaggtgcacg cgtgactgaa 180

tttaatggcg gtaaaagtat aagcatgggc aatggctcgg ttatgaaaat aaacccagac 240

ataccggtgg ttatagcacg gagtggttaa cgtttcacga ggcacgcagc cgaaatttgg 300

gctctggcga caaaccagac tatttccaat gcaaagctgt tgtgcatata gtaaaatgtc 360

agaatgcctt ctacaaggct tgtccgcaag cggaatgcaa caaaaaggtt atcgacgaga 420

ataacggaca ttatcgttgc gagcgttgta atgccgtctt cccaaacttc aaatacaggc 480

tactaattaa tatgagcatt ggcgattgga catcaaatcg ttgggttact tgcttcagcg 540

aagtggctga acaactgttg aagcgttcgt cgcaggaaat cggtgattta ctcgaaaaca 600

atcccccaga aggtgaagaa gtgctctcct ctatcaactt caattcgtat atatttaaat 660

tgcgcagcaa agtggaacag tttggtgata tgcaacgtaa taagctgaca gtacctcggc 720

c 721

<210> 169

<211> 229

<212> DNA

<213> S003(1)-A10

<400> 169

gtgccaagct tgccacacaa tacctttggc aaactgatct tttggaaatt ctcctcctat 60

atataaaccg ttaagattgc tgctttgaca tttccaatac cacccagctc ctgtatattg 120

tacctcggcc gcgaccacgc ttgacgatgg aggcaatcat gtcggtggtg aagctagcac 180

aaatctcttt gacttcagtt acgacccatg tacctcggcc gcgaccacg 229

<210> 170

<211> 553

<212> DNA

<213> S003(1)-B6

<400> 170

taacagcagt tctcgttcta caaataattt aacgatctac aaaatggcca gccaagagga 60

atttgataaa gccgctgaga atgtaaagaa tctaaactct acaccatccg ataatgattt 120

attggaactt tacagtttat ttaaacaggc tactgtagga gattgcaata cagataagcc 180

tggtttcttg ggcttcaagg gcaaagctaa gtgggaagca tggaacactc gcaaaggtac 240

tgatgttttt gcacgtgaaa cttcttttct taagcgaaat ataaaacgtc atccatttag 300

accatgttat gtagctgcta tggataaacg agctatagaa gaaacatgca attggatttt 360

tgaaggtcat gaagaacctg aagcgtctat catagcttgt gaatcaatgt tacttaatgc 420

atttggtcat ggtccagaat attataatca tcttagaaat aaagtagttg tattctggcg 480

tcaattaaat atttatccta gaattccttt atggatagaa gtagattgga gaatctacga 540

aggtacctcg gcc 553

<210> 171

<211> 362

<212> DNA

<213> S003(1)-D7

<400> 171

atccgatacc gcttacaacc attaatcttt gattaaaatg ttgaataaac ttaccgatca 60

atattcgatc ttcattctac gcgatgttgg tgatggtaaa acttgctttg cctatcaggc 120

taagatagat aatttaacca tctgtctctt cacaagtcaa ttgggttgtt gccacccttt 180

ttgtaagacc atatacaagg aaagaggagc atttaatgat agaacttatg ttacggataa 240

attactcaaa gctttcttta tggcacttga cttatatctg atgcatgatt gtctcttcaa 300

acgtgatgtc cttacaattt accagccgcg ttctggtgag atcaattata tgtacctcgg 360

cc 362

<210> 172

<211> 435

<212> DNA

<213> S003(1)-E1

<400> 172

aatttacact ctgatacttc cactcaaact gaaacaaatc attggtatag attaccattg 60

cacataagga taagaattgt gcagctagcc gatatcaact gtgaaagcaa atatgagctt 120

ttacgtttca attttgactg tttggcatcc ctatggaacc atgatcaaat ggctaatcgt 180

tgttgtggaa tgttctatct ccaagcacca aatgagatag aagtcgctaa tatgcacgac 240

gttcgtttac actctacaca accaggaaga aaagaaccat gtttgtgttc atcattattc 300

tggtatgata gcgcgctgat gcaatcagct attgatctgt ttcatagtat taaacagtgt 360

gaagttgtcg atgaggagta ttatattgtg ttggattgat attctctccc atgtacctcg 420

gccgcgacca cgcta 435

<210> 173

<211> 211

<212> DNA

<213> S003(1)-E9

<400> 173

cagagaagtg ctttcataca aattagggtg tttctggatt ttgaactaaa ttttcattaa 60

atatgagttt ggcgggtaaa aatgttgtgt tcgttggtgg cttgggcttc attggttatg 120

aagcttgcaa agcgttgatg accagagatt tggcggtaag acggaagaga atttccaaat 180

taaaaatacg caaaaaaaaa aaaaaaaaaa a 211

<210> 174

<211> 380

<212> DNA

<213> S003(1)-F2

<400> 174

ttttcttaag cgaaatataa aacgtcatcc atttagacca tgttatgtag ctgctatgga 60

taaacgagct atagaagaaa catgcaattg gatttttgaa ggtcacgaag aacctgaagc 120

gtctatcata gcttgtgaat caatgttact taatgcattt ggtcatggtc cagaatatta 180

taatcatctt agaaataaag tagttgtatt ctggcgtcaa ttaaatattt atcctagaat 240

tcctttatgg atagaagtag attggagaat ctacgaaggt accagttcta tttgctggac 300

gtgatcgctt tggtcgtagc cgccgtcgct gttgcccttg gagtgcttgt tctgttggta 360

cctcggccgc gaccacgcta 380

<210> 175

<211> 482

<212> DNA

<213> S003(1)-F5

<400> 175

atacctactg gcagtgtgta atcgtaatat ggtttaaggt cgaatggctt ttccccctct 60

ttcggccgat gttcgcgatc aaggaaaggt attaatgggt ataaacgcat tacttcgtcc 120

actaccatac ccagatatgg tagattattg atagtctcat atgacatttt accctgttca 180

gctacaaaag cttcacatat ctcattgcgc agacgttctt gtaaatcggg tcgtttggat 240

aattcgtata gcgtaaatgt catcaacgaa tatgacgact caaaaccagc tgagaaaaac 300

acagcagctt gtgctgtcaa aacatcctgt agatgggcat tatactcgcc tttcgcaaca 360

gcctctcctt tgaggctcac caacacatcg atcagatcgt tacgcgttgc tttgctctcc 420

tctcttgacg ccatcacatg attgatggtg ccacgtaaaa aggtacctcg gccgcgacca 480

cg 482

<210> 176

<211> 465

<212> DNA

<213> S003(1)-F9

<400> 176

tccgataccg ctaacaacca ttaatctttg attaaaatgt tgaataaact taccgatcag 60

tattcgatct tcattctacg cgatgttggt gatggtaaaa cttgctttgc ctatcaggct 120

aagatagata atttaaccat ctgtctcttc acaagtcaat tgggttgttg ccaccctttt 180

tgtaagacca tatacaagga aagaggagta tttaatgata gaacttatgt tacggataaa 240

ttacttaaag ctttctttat ggcacttgac ttatatctga tgcatgattg tctcttcaaa 300

cgtgatgtcc ttacaattta ccagccgcgt tttggtgaga tcaattatat gcacggaaaa 360

tacctacgac ggttatactt cacaaagatg actaatactg gccctggaaa agctagattt 420

actgcatttg aagtagctcc tatggtacct cggccgcgac cacgc 465

<210> 177

<211> 497

<212> DNA

<213> S003(1)-G4

<400> 177

acccatacct gcaacggcta ccaaacgact ttctcacaaa attccaccac cccgatagac 60

gctataaaaa acaacttttc cttggcctat atttcgatgt aatctacgag cgtatacgta 120

aaacattgca cacgaagtct gctcctttct tacataaagc caacttcgtg gacgatttct 180

tcataagcca tgcgcaattg caagcagtga agaaattaag ttggaatttt tttgtagcac 240

atgatgacct tgatgcatgg accgaactca agcggaacat attagcacat cgaaatacca 300

gcaaagctga tctggacatt tatgttggca gtcatggcac gcaggtgtta aatggcccta 360

atggtgaaca aatgaagctc tacctacatc cagaattggg agagtatggg aaattccccg 420

tgccagaact cttgtggatc gttgtgaagg aggaagataa atcaacggct tttggtgtgt 480

acctcggccg cgaccac 497

<210> 178

<211> 403

<212> DNA

<213> S003(2)-C6

<400> 178

tcgtaggttc agctgtgcag tagcaaaagg cttcagagga ttcggttaat gtgcgttcat 60

agcttcaaag aagttttctg cttttccagt ttgattgagt ttataatttg taataatttt 120

ctaaatattt ttttatagtt taaagaaaaa tttagcgcaa attaagagaa atcgtaattc 180

gataatttgg atttaaggta agaatatgct gaaataaaag ccataattta tgaaaaataa 240

aacaagagta aaaaaatgct tgttgttgga gtgcaacgct atcttgcaga ccttaaaaat 300

cataattaat aaagagttag ttcttaaaaa aaaaaatatt ttagcattca agcagacagt 360

gtttaatgat gtaatattac caaaaaaaaa aaaaaaaaaa aaa 403

<210> 179

<211> 451

<212> DNA

<213> S003(2)-D4

<400> 179

atacgcgcaa tacccacagc gcggaagaga ttgatggcca gaccaggggt tgggtcgatc 60

agcacgtaga ggaagccaat gatgaagaat ggcaaaatgt tttccagatc attgcgatga 120

gctctgcgca cgcgttcaac atcgggatta tcgaatttca ctttaaggcg tttgtccatc 180

aaatcctcgg ggttggcgaa ggtctttgtc cggaagcgtt gaattgccgt cagcaatgac 240

atgaacaaaa gttttagaac caaaactgcc gaccagaaga cataggcact gaatacttca 300

ttatcaaacg atatcaaatc ttttgccgcc attttacttt gactaaatga acttctttaa 360

ttttctagaa aaatataaaa atgtctacga ccggtaaaat ttactgatct atcgcctcag 420

aaatcaactg tccaactccc catgtacctc g 451

<210> 180

<211> 662

<212> DNA

<213> S003(2)-D5

<400> 180

gttgctcggc ttgttacgca cagcagcggt gcgttggcaa aaggaaacga gaaaaaaatc 60

gaaactgtca gtcacacccc tgagcgtgaa aaatgtgtgc gacaacgaaa caccaccagc 120

gcacaaatgc acacttctgc gttgctgacg gtgggtgaac agacagacac ccaaagaatt 180

cttcaaatat ttgtaaaccg ttttaggaaa ttcgacgact tttacacagc caattttttt 240

gggcgcagct taaggcgact atatacgtaa agactcgatg acaaatgccc taaaaagtct 300

cgagttccga attcgtcacc ttcagcacta tgcaagtatc cccaaaattc actcttatgc 360

aaaacgtgca aaagttttgc atttatcttc gtcgccacaa aaaactagtt tacacaacac 420

acgccttcat taatttcaga attatcgtct tagttcactt taatctcgac attttcttac 480

atttcaacac ttggtcattg acaaatctct tgcttggcaa gtgcaaaaaa gaggaatcaa 540

attactttgc ttactttgca atcaactcat tcggttgttc gcccgttcgt tagctacgcc 600

tcggttcgtt tgattgaaac gcttacaaat gtcgacgacg acagtctccc atgtacctcg 660

gg 662

<210> 181

<211> 719

<212> DNA

<213> S003(2)-E7

<400> 181

gcttcacaat ctcattgcgc agacgttctt gtaaatcggg tcgtttggat aattcgtata 60

gtgtaaatgt catcaacgaa gatgacgact caaaaccagc tgagaaaaac acagcagctt 120

gtgctgtcaa aacatcctgt agatgggcat tatactcgcc tttcgcaaca gcctctcctt 180

tgaggctcac caacacatcg atcagatcgt tacgcgttgc tttactctcc tctcttgacg 240

ccatcacatg attgatggtg ccacgtaaaa aggtacagac tttatgaaac cagtgataca 300

tatcgtcgag gaacttctca acaacacaac actgcaggta ggtgtctact ccgggcaatt 360

ggacttgatt tgtgccactc caggcacggt taactggatt gagaaaatgc aatggaataa 420

taagtcttca tacgaggcgg caccacgcgt tggcatcagt gttaatcgca ttttggaggg 480

ctatgagaag accgctggca atttcactat gttctggatc gatcgcgctg gacatatggt 540

gcctgcagat aatccagctg gcatgggcca tattttaagg aaatatacca actttggttg 600

agagtaaact tcaaatacat atttgaaatg caatcaatat caaatcattg cataagaaat 660

cattaataaa ccttttgtta ttacaaaatc tataaaaaaa aaaaaaaaaa aaaaaaaaa 719

<210> 182

<211> 489

<212> DNA

<213> S003(2)-F3

<400> 182

aacgaagatg acgactcaaa accagctgag aaaaacacag cagcttgtgc tgtcaaaaca 60

tcctgtagat gggcattata ctcgcctttc gcaacagcct ctcctttgag gctcaccaac 120

acatcgatca gatcgttacg cgttgctttg ctctcctctc ttgacgccat cacatgattg 180

atggtgccac gtaaaaaggt actgaactct ggtgtaaaaa gtgtaatacg caatgtagag 240

gcccacttgg aaaagaaaaa accgacgaag aagtcaatgg cacgacggaa tgtagcggtg 300

aacaattttc gtccttgagt gcgaaattcg ccgtttggat tactaagact gttggctttc 360

acaccaaaag cgatggaggc aatcatgtcg gtggtgaagc tagcacaaat ctctttggct 420

tcagttacga aagcgtcgtc cgttttcgca tacgagttca aatgggcttc caaatccgta 480

cctcggccg 489

<210> 183

<211> 306

<212> DNA

<213> S003(2)-G5

<400> 183

acacaaattg ctgacctatg cgctggtggt cttggtgctg ctgcaagttc tgatcgaccc 60

caccgaagcg gcgacgaagt gtcacaaatg caccggaatt aattgtcaac gcacgactta 120

cgcagccacg gaggattgta cagacgcgct tgacagttgt gtcaccgttt ttcaagaatc 180

tatggtccta gcacaaggct gccttggtca attagccgta gagttacgta acaaatgtga 240

aatgccaagc gctgaggaga ccgacagaac tgaaatgacc gatgctgatg acgccgtacc 300

tcggcc 306

<210> 184

<211> 223

<212> DNA

<213> S003(3)-F1

<400> 184

aaatttggac ctgtaactag tccgttgagt ttacgtttta ctccaacacc acgcacaccc 60

gtctgttatg gaatgattgg tctttcagat cacttcattc ggctatatgt tgcctttgaa 120

gatccacagg acggatctga ctacacctct gaagactttg ggtgtaatgt ccagttaagt 180

ggaagcgtta cgctgtgttg tcgtacctcg gccgcgacca cgc 223

<210> 185

<211> 671

<212> DNA

<213> S003(3)-H10

<400> 185

gacacgctac tgttaaacag ttaaaccatg ccgttacgct atataggaag aacacaagga 60

ttcgcgtcaa tgactcatga cgagatattt ccaagtaaat tcgttttacc atttctggat 120

catattcctg ctaaaagacg ttcacccgtt acgcaaagcc ttattatcga cgatttaaaa 180

caatttaata ataccactaa aaggcccaat gatccattac ttgatttatc aatcgctcat 240

gctacgcgaa agtttctgtt gcccaatgca gttaaaatgt tacaccttaa tgatgtcttt 300

aaaagagaaa gtgacatctg gagtaaaagt cctggtcttc cgtggactcg atatggcgtt 360

aaaactaaga gacaaattcg tgatgaccca gacaaaatac aggaagtgag aaaattttgg 420

cattttgtga aagctggtga tgacataagc ttccccgatt gttgcgcttt cgtccgtgct 480

catgtttgtg gacttgatga gcataaagtt cgtgctgttt ggggatatcc agctactatc 540

acatttggag aggccgtatt cgctattcct ctcattgaag gataccaggc cctttcaccc 600

gatttgactc ctattgctta tggattggag actgcaactg gtggagccca gaagattcgc 660

aaccgattca g 671

<210> 186

<211> 231

<212> DNA

<213> S003(4)-A8

<400> 186

tgcttaccat gtactctgcg ttgataccac tgcttcccat gtactctgcg ttgataccac 60

tgctttccat gtactctgcg ttgataccgc tgcttaccat gtactctgcg ttgataccac 120

tgcttcccat gtactctgcg ttgataccac tgcttaccat gtactctgcg ttgataccac 180

tgcttaccat gtactctgcg ttgataccac tgcttcccat gtacctcggc c 231

<210> 187

<211> 444

<212> DNA

<213> S003(4)-C1

<400> 187

taccggcgaa ttgatcctga agtgcaccgg ccccagcgaa gccgtgatga tgatgtagag 60

cttagctcta ttaatggttc agagagagat taagttgaat atctctaaac agcaatatga 120

aggaaaacat caatgaataa aaccctcagt agatgagtaa aatatgaagg aaaacatcaa 180

tgaataaaac cctcagtata tgagtaatta aattgaatga aggaaaacat caaaataaaa 240

ccccatgatt ggtaatcatc aatatgaacc atccctttca cggtaaggga actatgttgg 300

gcttagtttc agttcaacct atccaataac cgaaggaaaa cgaacttaac ctgttgatta 360

cgtaaaatca ctgtcgaagg aaaacgttat aacccggaat gtccgttaat atcgaaggaa 420

aaaaaaaaaa aaaaaaaaaa aaaa 444

<210> 188

<211> 253

<212> DNA

<213> S003(4)-C12

<400> 188

gatgacgaaa ggtacttcgt gtatgccaca aattgattgc tctctagtca aaacaccaat 60

tttacttctt atgggtttaa ctctagctaa aacttgttgt tttacatcgt cttgcaccac 120

ttctagtaaa tctgttttaa acgataatgg cggatcggta tggtgtaaac agccattcac 180

cattggtgag ccctcaaatc gactatcacg atgtgatata tgaggaaagt cgtacctcgg 240

ccgcgaccac gct 253

<210> 189

<211> 347

<212> DNA

<213> S003(4)-F1

<400> 189

tgagaacgaa aataacgccg attttcacat caggaaagat caaacaaatg tattcattaa 60

tgactgagat cggcacggag ttggaagccc atttgaattc gtttgcgaaa acagacgacg 120

ctttcgtaac tgaagttaaa gagatttgtg caaacttcac caccgatgtg attgccacca 180

ccgcctttgg cattaaagcc aatagtctta tagatccgaa tgccgagttt cgtgcgctag 240

gccgaaaaca gctagacttt acattaggac gcgctattca attccttatc gcattcttct 300

atccgaaatg gaccacagca ttgcgtgtga aaattttggt acctcgg 347

<210> 190

<211> 210

<212> DNA

<213> S003(4)-G6

<400> 190

ccaaatctat ccgcgttcat ttcgtgacag tgatggtgac ggtattggtg atttaaatgg 60

tgtcactgag aaattgcaat atctgaagga tatcggtttc acagcggctt ggctgtcgcc 120

gatcttcaaa tcaccgatgg ccgatttcgg ttatgacatc tccgatttct atcaaattca 180

cccagaatat ggtacctcgg ccgcgaccac 210

<210> 191

<211> 545

<212> DNA

<213> S003(5)-B11

<400> 191

caattatggc cattgagcta agcacttttg gttctcgggc agacggcact ttacaacgct 60

ggtaatctaa caaaaccgca tcgagtagtg gcggaataaa gttatccata accaattgat 120

tgtcattaga acgcgatacc cactccgaaa ttaaattcaa agtctctttt tttacaacat 180

tcattgattt tataagcggt tgattattta caacgacacc gtttacctca atcgcctgaa 240

ttatgttctc agatgttatt ttatagacat taagcatatc caaatatatg cgtcccaact 300

gaattacata ggcatgccct aaagccttac atgcggctac attcgtcttc aaaatgctac 360

ccagctgctt tacagctgtc atatttttga gaaagtcaac attcttggag gcacgtgaga 420

ttatatcatc ccaaacctgg tttggcgaga acatatactt ctcaattaag gcgtcctgct 480

gtacttgatc aatctgtgcg gatatcatgt agcccactgc ttcgtagaac gtatgtacct 540

cggcc 545

<210> 192

<211> 116

<212> DNA

<213> S003(5)-B2

<400> 192

atattgtggt ggggatttaa attgcttgaa atttcaatta attgtctaaa tactgagcta 60

gccgtagcta aattaactca ccttagggtc acgatgtacc tcggccgcga ccacgc 116

<210> 193

<211> 702

<212> DNA

<213> S003(5)-B8

<400> 193

gcgcgtgcgc atgatgaacg agattgtctc gggcatacag gtgatcaaga tgtatgcgtg 60

ggagaaacct ttcggcaaga tgatacagtt tgtacgtcga aaggaaatgt cagccatacg 120

ctacaataac tatatacgcg gcatcttgca atcgttcgcg atgtatatga cccgcgtatc 180

tgttttcgtc agcttagtcg gctatgtgct gttgggtaac tttctcaccg cggaaaaagc 240

cttcgttatc actgcctttt acagcatttt gcgcaacacc atggttgtgg cctttcccaa 300

tggtattcaa caaatctcgg aaacgcttgt gtccatgaag cgtataacag attacttatt 360

atacgaagaa atcggtgata actcgaaaat ttcagcgaat agctgtaata aaactaagaa 420

gacagacgac ctgaatggta atctaaagtt aacgaaacaa ataacacagg agccagcacg 480

taaagaagct ggcattgaaa tatgtaattt gaaagcgaag tgggatccca ccgcaataga 540

atatacgctg aacaatgtga acttgaatgt aaaaccgcgc acactagtgg ctgtcattgg 600

accggtgggt tccggaaagt caagtttgat acaagccata cttggtgaac tgcctacgga 660

atcgggttcc attactgtac acggctccta ctcgtacctc gg 702

<210> 194

<211> 516

<212> DNA

<213> S003(5)-E6

<400> 194

gatgaaggac tttcgatcac aaaactggac ataaaaccag ccacaaaaaa gggtgacaac 60

tatgccagtg tgatgacacg aatcagcgtc gatttcacgt taagcactaa ggataccgaa 120

catggtttat atatcgtgaa gacctcacat gaaagtgatc ccttcacagc aggtatcatg 180

agtcaatatg atatctataa tacggaaatg ttaatgtaca aaaaagtctt accaaaactg 240

accgagttgc taaaggaaat cggtgacaca gataaactat ttgccgacac cattcatgtg 300

gactacaagc attcagctat aattttcgaa gaccttgcag tgttaaaatt tgttactccc 360

gatcgtttag ctgggatgga tgaagcgcag accttgttga cacttaaaaa actggccaaa 420

atgcatgcaa ccgcggccgt accataggag ctacttcaaa tgcaataaat ctagcttttc 480

cagggccagt attagtcatc tttgtgaagt acctcg 516

<210> 195

<211> 193

<212> DNA

<213> S003(5)-H5

<400> 195

taatctacca tatctgggta tggtagtgga cgaagcattg cgtttatacc cattaatacc 60

tttccttgat cgcgaacatc ggccgaaaga gggggaaaag ccattcgacc ttaaaccata 120

ttacgattac acactgccag taggtatgac tcttttagta tcagtcctag gcgtacctcg 180

gccgcgacca cgc 193

<210> 196

<211> 456

<212> DNA

<213> S003(6)-8

<400> 196

gtattggcgt cataggatcg ccagtcatta cattgtgatc cctacatttg aatccctcca 60

gactaagcgc actacttgtg gccacactta ataatgcaat taaggctatc tttaatgcca 120

tttcgtttcg ttgatttggt gcgtcaacta gcacaattat ttatttataa aatgtgtgta 180

ttaatacgcg tcctatgcaa acaaaattga tatttgaaaa cagaaattgc aacaatttta 240

agttagatac tttcacgagc agtgatcgca agagactgtc cccatgtact ttatatgcgt 300

caacggagac acgcgtgaga gcgccaagtg ctggatattt gcggagagtt tcatttataa 360

cttgatccaa ataggtcatc tcttccatgg catcatatgt caactcgcca ttgtgtttac 420

ttagcaccgt tttaatttcc tctctcaact tatcct 456

<210> 197

<211> 709

<212> DNA

<213> S003(6)-32

<400> 197

atggggactt agtgttattg tgaaagcgcg ttacaaaagg tcgacagaat ttaataactt 60

tcataccaga ttttttctta agcgcttcga aagcagtgcg cataatgctg tcgataaata 120

ctactttcct cttggcggct ctcttggggc ttgtctatgt ttggtgtcgc tacagttttg 180

gttattggaa acgaactaat attccatata tgtcgccact gattggcaat acggaagttt 240

ttattaaaag gaccaatagt ttcttcctat atctagctga tgtttataag gatgctaaaa 300

tgttaacagc tgcggcagtt ggtatttaca tactcaacaa gccagcgtta gtgttacgtg 360

aaccggaact tattaaaact gtgctagtca aggaattcgc aaagttcgaa aatcgatcag 420

ccgcctgtga tccacacaat gatcccttgg gttcgaataa tctatttttc gtaaacaatc 480

cactatggaa ggatttgaga acgaaaataa cgccgatttt cacatcagga aagttcaaac 540

aaatgtatcc actaatgact gagatcggta cggatttgga agcccatttg aattcgtttt 600

gcgaagacta acgacgcttt cgtaactgaa gttaaagaga ttttgtgcta gcttcaccac 660

cgacatgatt gcctccatcg cttttggtgt gaaagccaac agtcttagt 709

<210> 198

<211> 461

<212> DNA

<213> S003(6)-51

<400> 198

atggggcgtt tttatgacac attggatttc cgataccgtc aatgaaaatt ctttcataat 60

aaaacctgcc gagttaaata ttattcaact tttaaatatt tatcactcaa ctgaaacaaa 120

aagttaaagc catggaatgc acaaaagaga tcacatgtct acagaactta actcaacaag 180

ttgctgagtt atcaaaagcg ctatcctaaa atatgcagca tacgttgtcc ctggagggaa 240

aattttcagc tgccatggaa gaaatcgcta aactccaacg gcaaaacgcg atgatgctag 300

atgtcatcaa taaaacctta tatagtaata ataaagagaa agaaaataac aatgactatg 360

gttttggaat atttattcta acgatgatac ttgtcctaat tggacttcta cgcttggttc 420

ccacatccgt ttggcttggt ttggaggata aagtatactg t 461

<210> 199

<211> 382

<212> DNA

<213> S003(6)-74

<400> 199

agttttgagc tcgataagag aggtgttaaa gaaagaatat ttggataatg ccaaagcgat 60

ttcatccgcc tacaaaaatc gtccgcagag cccactcgag actgccattt ggtgggtgga 120

atatgtcgcc aaaaccaagg gtgcgccact cattaaggct catgccgtgc atgtatcacg 180

cttcgtttac tactctttgg atatttatct attcattgct gccattatat tcataacggt 240

tgtgagttgg agctttatat gtagtagatg gtgctcgcgg cgtccaaagg ccgttaagca 300

aaagtcaaat taaacaagga ggtgcgaatt tcagacgaag agagttagct caataaaatg 360

taaataagaa attttaattt ga 382

<210> 200

<211> 359

<212> DNA

<213> S003(6)-81

<400> 200

tattggatat ttacgaagtg tttctgatac gacctgcttc atataagaca tcgaatttac 60

acattcatag gtcaccttac cgccatgatt ttcaaccgcc tctagaattt cttcgcgtag 120

gcgcttttga atatctggat ttagggccaa ctcatagagt aaaaatgtaa gagtcgaaga 180

tgatgtctca aagccagcta caaaaaacac aaacgcctgc gccgtcatct cctccaacgt 240

aagccctttc tcctcgtttt tatcgtcgaa ctttaatttt cctttgttta attcaatcag 300

aatgttcaaa aaatcattac gctgcacatt cttcttccct ctataatcaa ctgcttcac 359

<210> 201

<211> 341

<212> DNA

<213> S003(6)-92

<400> 201

tgcatgcctc ttggcggctc ttttggcgct cgcctacgtt tggtgtcgct acacatatgg 60

ctactggaaa cgcaataaga ttccctttat gacgccattg ccactaattg gcaatatgga 120

agttttgttt aaaaggacca atagtttctt tctatatcta tctgacattt ataaggatgc 180

tagaatgtca aaggctgctg cagtcggcat ttacatactc aacaaaccag cgttagtgct 240

acgtgaaccg gaacttataa aatctgtgct catcaaggag tttccgaaat tcgccaatcg 300

gtcaggcggc tctgatccac ataatgatcc tttgggcgcg t 341

<210> 202

<211> 377

<212> DNA

<213> S003(7)-151

<400> 202

caaaattttc acacgcaatg ctgtggtcca tttcggatag aagaatgcga taaggaattg 60

aatagcgcga cctaatgtaa agtctagctg ttttcggcct agcgcacgaa actcggcatt 120

cggatctaca agactattgg ctttaatgcc aaaggcggtg gtggcaatca catcggtggt 180

gaagtttgca caaatctctt taacttcagt tacgaaaacg tcgtctgttt tcgcaaacga 240

attcaaatgg gcttccaact ccgtgccgat ctcagtcatt agtgaataca tttgtttgat 300

ctttcctgat gtgaaaatcg gcgttatttt cgttctcaaa tccttccatt gtggattgcg 360

tataaaaaat agattgt 377

<210> 203

<211> 521

<212> DNA

<213> S003(7)-160

<400> 203

cttggcagtt atttcaaacg ctagacattt aatcgttaat ccataatttc ttctaaggcg 60

agcatgccac tgctcttggg ataaacgact atcaagcgac ggtttctgaa tatataatcg 120

ccgtaagtat tttccatgca agtaattaac ttcactagaa cgcggacggc aaaatgaaag 180

aatgtcacgt ctaaagaggc aatcacgcat cagatataag tcaatagcca taaagaaaac 240

ttttaacaca tctttcgtta ttatatatct atgtaaattt gttattgagt tttcactgca 300

tatggtctta caaaatgggt ggcaacagcc cggttgactt tcgaaaagac agaaaactga 360

acatattcgc ttaactctat aagaaaagca agtctcattt tcaccagcat tgcgttggat 420

gaagatcgga tgttgatcag caggttcttt caacatttta atcagggatt aaaggttgta 480

agcggtatcg gaaatccaat gtgcttaaaa acgccccatg t 521

<210> 204

<211> 454

<212> DNA

<213> S003(7)-165

<400> 204

gttttcacca aacctataat cattgtgttg gacttagact tggcaaaaac gatactaatc 60

aaggatttta acaaatttac ggatcgcttt caacggcgta agcccgataa gcacaggttc 120

gacattctca atgagacact atttcgtgtg tccggtgata ggtggggtcc attgcgcaag 180

aaactttcat cgaccttcac atcgttgaag acgaagttca tgtttccgac ggtgttgaac 240

gtggcgcaac aaatggacga agctctcaaa gcgcacttcg caaatacgcc gagcggtgac 300

atagatacac acgattggat gggccgcttt acaaccgatg tgattgctaa ttgtgccttc 360

ggtgttaagt gcaatagctt aatagatcca aataatatat ttcgtcacat aggacatcgc 420

attttttatc caaaatacgt atccctaaag ctgc 454

<210> 205

<211> 340

<212> DNA

<213> S003(7)-167

<400> 205

atgggaacag caatatgaag gaaaacatca atgaataaaa ccctcagtag atgagtaaaa 60

tatgaaggaa aacatcaatg aataaaaccc tcagtatatg agcaattaaa ttgaatgaag 120

gaaaacatca aaataaaacc ccatgattgg taatcatcaa tatgaaccat ccctttcacg 180

gtaagggagc tatgttgggc ttagtttcag ttcaacctat ccaataaccg aaggaaaacg 240

aacttaaccc gttgattacg taaaatcact gtcgaaggaa aacgttataa cccggaatgt 300

ccgttaatat cgaaggaaaa aaaaaaaaaa aaaaaaaagt 340

<210> 206

<211> 492

<212> DNA

<213> S003(7)-175

<400> 206

cgatctcagt cattagtgga tacatttgtt tgaactttcc tgatgtgaaa atcggcgtta 60

ttttcgttct caaatccttc catagtggat tgtttacgaa aaatagatta ttcgaaccca 120

agggatcatt gtgtggatca caggcggctg accgattttc gaacttgcga attccttgac 180

tagcacagtt ttaataagtt ccggttcacg taacactaac gctggcttgt tgagtatgta 240

aataccaact gccgcagctg ttgacatttt agcatcctta taaacatcag ctagatatag 300

gaagaaacta ttggtccttt taataaaaac ttccgtattg ccaatcagtg gcgacatata 360

tggaatatta tttcgtttcc aataaccaaa actgtagcga caccaaacat agacaagccc 420

caagagagcc gccaagaggc aagcagtatt tatcgacagc attatgcgca ctgctttcga 480

agcgctgtat gt 492

<210> 207

<211> 656

<212> DNA

<213> S003(8)-196

<400> 207

catttggcgc caaatttaag taacggcacg catcggtcgc aaagatggtg atttcttgct 60

tagagcttaa atgtggtggg aataaatcgc cggtggtgcc attaacggtg ccacatggtg 120

cataataaaa gccagtctca ttcttcatat tccaatgcgt caagcgaccc aaattagcca 180

tatcatcgac accagtatgc attgtgaata gaccgtcata ttcggccgaa ccattacggt 240

caaccaacca gccaaagcga gtgtatggta tctttatatt tgttgtattg aaaaggttta 300

aaaaatcgat aagcctatcc ttgtatcctt caaagatcca ctcgcgcact gttttcgttg 360

tgtaccctgt tcagctacga aagcttcaca gatctcattg cgcagacgct cttgtaaatc 420

gggtcgtttg gataattcat atagcgtaaa tgtcatcaac gaagatggcg actcaaaacc 480

agctgagaaa aacacagcag cttgtgctgt caaaacatcc tgtagatggg cattatactc 540

gcctttcgca acagcctctc ctttgaggct caccaacaca tcgatcagat cgttacgcgt 600

tgctttgctc tcctctcttg acgccatcac atgattgatg gtgccacgta aaaagg 656

<210> 208

<211> 387

<212> DNA

<213> S003(8)-204

<400> 208

actttcgatc ctgaacgctt caacgccgag aataagaaaa cccataagcc tatgacctac 60

tttcctttcg gcagcggacc acgtaactgc attggttctc gcatcggttt gttgcaggcg 120

aaggtcggtt tggtgcatat ttttaagaat cattatgtga ccacatgtga gaagacaccg 180

tccgaaatca ttttcgatac actgggcgta gcgctaaatt ataagagtgg aatgtacttg 240

aactttgtca atgacaaacg ctatgaacgc aatgccaagc agtgaatgtt ggaaattcga 300

atgaattttg agatatcaat tttaattttt ttgaaataaa aaccaaactt cttgtcataa 360

aaaaaaaaaa aaaaaaaaaa aaaaaaa 387

<210> 209

<211> 575

<212> DNA

<213> S003(8)-220

<400> 209

ttgtcttacc tgctgaagaa gacttagaag atgatcttct ctcagatgaa gagtcggttg 60

aagcagagac tgattcaccg gcatcatctg aataatggag ccacttaacc gacaaagtag 120

ttatatgaaa agtggatctt gaagtttacg agtataagta catcgtgacc ccaagttttg 180

gcccaacccg gaaactttcg atcctgaacg cttcaacgcc gagaataaga aaacccataa 240

gcctatgacc tactttcctt tcggcagtgg accacgtagc tgtattggtt ctcgcatcgg 300

tttgttgcag gcgaaggtcg gtttggtgca tatttttaag aatcattatg tgaccacatg 360

tgagaagaca ccgaaggaaa ttacttttga tccattttta atgtcgctag gttataagaa 420

tggaatttac ttgagccttg tcaatgacaa acgctatgaa cgcaatgcca agcagtgaat 480

gttcgaaatt cgaatgaatt ttgaaatatc aattttaatt tttttgaaat aaaaaccaaa 540

cttcttgtca aaaaaaaaaa aaaaaaaaaa aaaaa 575

<210> 210

<211> 610

<212> DNA

<213> S003(8)-224

<400> 210

cagcggcgac aatcaacaca gcacaatcag cctgtgaggt acaggttgaa cagtttaccc 60

accactatca tctgttattg cacacggagg agcttcagtt gacctagcta ttttttcact 120

tcacttagcg ggtatttcct caattttagg agcagtaaat ttcattacaa cagtaattaa 180

tatgcgatcg acaggaatca cctttgatcg aatacctcta ttcgtttgag cagttgtatt 240

aacagcttta ttacttttat tatcattacc agttttagcg ggggctatta ctatactact 300

aacagaccga aacttaaata cttccttttt tgaccctgcc ggaggaggag atcctattct 360

ttaccaacat ttattttgat tctttggaca cccagaagtt tatattttaa ttcttccagg 420

attcggaata atttcccata ttattagtca agaatcagga aagaaggaaa catttggatc 480

tctaggaata atttatgcta taatagcaat tggtctatta ggatttattg tatgagctca 540

tcacatattc acagtaggaa tagatgtaga tactcgtgcc tatttcactt cagctacaat 600

aattattgcg 610

<210> 211

<211> 318

<212> DNA

<213> S003(8)-231

<400> 211

aactgccgca gctgttgaca ttttagcatc cttataaaca tcagctagat ataggaagaa 60

actattggtc cttttaataa aaacttccgt attgccaatc agtggcgaca tatagggaat 120

attagttcgt ttccaataac caaaactgta gcgacaccaa acatagacaa gccccaagag 180

agccgccaag aggaatgtag tatttatcga cagcattatg cgcactgctt tcgaagcgct 240

taagaaaaaa tctagtatga aaggtattaa attctgtcga ccttttgtaa cgcgccttca 300

caataacact ccccatgt 318

<210> 212

<211> 598

<212> DNA

<213> S003(8)-249

<400> 212

agcaaattgg cagctttcac tcatttcaaa aactttcgtg agctggcatt tctccagttc 60

ttaaattgct gctgactgct ttgttgtttt catcattgtt ttataaaaat tatttaactt 120

cacgtactta tatcacatgg gtcacattat caacctttaa attctcctgt ttccactatt 180

cataactacc acacaatgcg cttatgtata cataaatata tgtatatata tagcaatatc 240

catataatgt ccgagacatt taatccttaa actaagttaa ttgattttaa tttaattact 300

aaatagacgt taaaacaaat tcgtatgatt caaatgcatg aggttatggc taatacgtat 360

gtatagtatg taaattaatg aaatttcacg tgagcctaag tcaaatggtt tattaccaat 420

ctctcgcata cgtctttatc atatcattgc tgatcagtaa tcgggttcac aatatgattt 480

gagctccgga tagccgcacg gacggcgaca gcactcgtgt atggcacctt gaaaaggttg 540

ccgatattca ccttcctcac cggctaaccg cttcaagctg gacaggtgtc ccatgcca 598

<210> 213

<211> 248

<212> DNA

<213> S003(8)-271

<400> 213

tcgttctggg taaatagaga atttcggttc ttgttccccg gatagtagtt acgtagccaa 60

ttgtggaact ttcttgctaa aatttttaaa ggattatatc gtaagatatg tattcttttt 120

aaattataac gattatcaat taacaatcaa ttcagaactg gcacggactt ggggaatccg 180

actgtctaat taaaacaaag cattgtgatg gccctaacgg gtgttgacac aatgtgattt 240

ctgcccat 248

<210> 214

<211> 460

<212> DNA

<213> S003(8)-284

<400> 214

atctttcgtc gcggtaatat tctggcaatg aagcgagcaa cttattgcgc aaggtcttga 60

catttgcgtt gttttccaag taaaatgttt tgcattgatc atttacaatg caaacttcta 120

tggaagactt tgcactttga ctgataattc cattacactc tttatcactc actatcatcc 180

ttggttcttg tcttcgctca atggcacggt tgctgtccat aaaaatgtcg tcctctgtca 240

gtcctagact tacacgtgcg gctgcatacg taacaatttc ggagtcaaaa gtcctgtata 300

cgtttaaacc atttcttacg aaacaaatta cgacagacgc acaagaataa ttggcaacaa 360

atgtcaacaa catgaaatat agtaacgcag acttgatctt catggtaaac gattcaagtg 420

tgacttgatt gatagaattt taaatcgcaa ctccccatgt 460

<210> 215

<211> 672

<212> DNA

<213> S003(8)-288

<400> 215

cgaaatatag gtctaggatt taaaactcct gctgaggcta ttgatggtac cggcattatc 60

agatagaaaa gaatacagca atttacactc tgatacttcc actcaaactg aaacaaatca 120

ttggtataga ttaccattgc acataaggat aagaattgta cagctagccg atatcaactg 180

tgaaagcaaa tatgagcttt tacgtttcaa ttttgactgt ttggcatccc tatggaacca 240

tgatcaaatg gctaatcgtt gttgtggaat gttctatctc caagcaccaa atgagataga 300

agtcgctaat atgcacgacg ttcgtttaca ctctacacaa ccaggaagaa aagaaccatg 360

tttgtgttca tcattattct ggtatgatag cgcgctgatg caatcagcta ttgatctgtt 420

tcatagtatt aaacagtgtg aagttgtcga tgaggagtat tatattgtgt tggattgata 480

ttctctccac attgcactcg aaggaaaacg ttgttataac ccggttgctc cgttatctca 540

aaataaaata atcgaaggaa aatgttacta taacccggtt gttccgttat cactactcaa 600

ttgaaggaaa acaattcaaa ccactatccg gttaaagata gtaaatgaag gaaaaaaaaa 660

aaaaaaaaaa aa 672

<210> 216

<211> 688

<212> DNA

<213> S003(9)-311

<400> 216

cgaagggaag gtaggacata ggcttatggg ttttcttatt ctctacgctg aagcgttcag 60

gatcgaaggt gttcgggttg ggccaaaact cggggtcacg ttgtatgccg aagattggta 120

tataaacagg catatctact ggcactgtgt aatcgtaata tggtttaaga tcgaatggct 180

tttccccctc tttcggcaga tgctcccgac cgagaaaagg tagtgatggg tataaacgca 240

atacttcgtc aacgaccata cctaaatatg gcagattatt gatagtctca taagacaatc 300

taccttgctc agctacgaaa gcttcacaga tctcattgcg cagacgttct tgtaaatcga 360

gccttttgga taattcgtat agcgcaaatg tcattgtcga agatgaagtc tcaaaaccag 420

ctaccataaa taccgctgct tgtgctgcta agatatcctg tagctgagca ttatactcgc 480

ccttcgcaac tgcctcttgt ttaaggctca ccaacacatc gatcagatcg ttacgcgtcg 540

ctttgctctc ttctcttaac gccataacat gttcgatggt gccacgtata aaagtttcgt 600

actccggtac gctgcacttc ctcatcggcc aacacactct gcaccagcgc tttgctttgt 660

accaactcat agtcggtcag ctttaggt 688

<210> 217

<211> 461

<212> DNA

<213> S003(9)-312

<400> 217

atggggcgtt tttatgacac attggatttc cgataccgtc aatgaaaatt ctttcataat 60

aaaacctgcc gagttaaata ttattcaact tttaaatatt tatcactcaa ctgaaacgaa 120

aagttaaagc catggaatgc acaaaagaga tcacatgtct acagaactta actcagcaag 180

ttgctgagtt atcaaaagcg ctatcccaaa atatgcagca tacgttgtcc ctggagggaa 240

aattttcagc tgccatggaa ggaatcgcta aactccaacg gcaaaacgcg atgatgctag 300

atgtcatcaa taaaacctta tatagtaata ataaagagaa agaaaataac aatgactatg 360

gttttggaat atttattcta acgatgatac ttgtcctaat tggacttcta cgcttggttc 420

ccacatccgt ttggcttggt ttggaggata aagtatactg t 461

<210> 218

<211> 265

<212> DNA

<213> S003(9)-321

<400> 218

atggttacca tttggcgatg gaccacgcaa ttgcatcggc ttgcgtttcg gcaaaataca 60

ggcgctgatc ggtctcgctt tgctgatcaa gcattttaga ttctccttcg caccacagac 120

acaagctgaa ttgacttaca aaaaagagac gatactgcta tcagcggaac atggcataca 180

cttgcgggtg gaggcgttga aagagtaatt gtataggtgt gctaggcagt cgtggagcaa 240

cgagcgggct gtcttcatct tatgt 265

<210> 219

<211> 233

<212> DNA

<213> S003(9)-352

<400> 219

ggaggcagca taattgaggg tttgtgcctt gtcgctccag tctacggaga aaacgttgta 60

tttaccactt gccaagtaag cgtcacggat caatgggttc aaatcggaat cagaatccga 120

ttgccaaccg tggattatga atttggttgg attgctttgt tgaagtgaga tgcagccaag 180

gaagcggcat cacccgaaat gatagtgtct ggcgtggcgg ggttctcgtt ggt 233

<210> 220

<211> 646

<212> DNA

<213> S003(10)-395

<400> 220

gtcttcgggt tgcgttaaat tcatgccggt taagagtgac aactcgctca gcatgccgag 60

gtatggttca atttccgctt ttacatttgg caactctaaa acttcgccta aagcctcaaa 120

ataacgcgga caaggtgtgc gcaccaacag taaagaatcc tcattgagtg gctgcgaaaa 180

taccggcacc ggctgccaat tatatttcgg atgccattcc atgggcgtat cttttggggg 240

gaacaaactc gccagcaccg tctgcagtgt catgtgggtg cgtgcaacac ccgttgcttg 300

agcgtgtgcc acgtctggcg tatagtatgg cgacatgaag tctgcgtaac gcttgcgcag 360

ccacgttcca atgccaaata gctcacgctt gccgttattg gttatgtgcc cccatccgaa 420

aggagagaag gtctggttaa tgtgtgggtc atttggatat gtgtctgcgg gtgtgcgtgg 480

tccgtgtcgg aaaaccacat gtagtaattc taaagtatca gcatcggctt tgtcttcgga 540

cacgaatgaa agaacgtttg aacttagcaa acataacaat aaaagcacca ccgccaccgc 600

tgcgccagcc atagctagac gatgtttatt agagaaatgt ccccat 646

<210> 221

<211> 462

<212> DNA

<213> S003(10)-404

<400> 221

aaatacacac gcatatacat atatgtgaga atttttataa aaaacaaaaa caaataaatt 60

aaagcgaaaa gcagctgtgt gtgttaattt acggcacgcc atatgcactt ctgcttgaat 120

tcatatggaa aaggttactc atacgtcgtg tagggtctga tggattgact ggtcataggc 180

gtatggaaat tttctttata aaaagtcatt tcgcaacgga ctaacgcgga aactgcaacc 240

tcctgtataa ctaaacaaaa aaaaaaaaaa aaaaaaaagt acggatttgt aaattatata 300

aaacaagaag atgctgagaa ggcaataagc tcactcaatg gcttgcgttt acaaaataaa 360

acaattaaag tttcagtagc ccgtcctagt tcagaatcaa ttaagggtgc aaatttatat 420

gtgtctggtc ctcccaaaaa tatgactcaa ccagaactcg aa 462

<210> 222

<211> 467

<212> DNA

<213> S003(10)-405

<400> 222

tattcgaaga aggtatgtta ccagacaaag atgccggcga attgatcctg aagtgcaccg 60

gccccagcga agccgtgatg acgatgtaga gcttagctct attaatggtt cagagagaga 120

ttaagttgaa tatctctaaa cagcaatatg aaggaaaaca tcaatgaata aaaccctcag 180

tagatgagta aaatatgaag gaaaacatca atgaataaaa ccctcagtat atgagtaatt 240

aaattgaatg aaggaaagca tcaaaataaa accccatgat tggtaatcat caatatgaac 300

catccctttc acggtaaggg aactatgttg ggcttagttt cagttcaacc tatccaataa 360

ccgaaggaaa acgaacttaa cccgttgatt acgtaaaatc actgtcgagg gaaaacgtta 420

taacccggaa tgtccgttaa tatcgaagga aaaaaaaaaa aaaaaaa 467

<210> 223

<211> 670

<212> DNA

<213> S003(10)-406

<400> 223

ggtccaaggt ctcaataaaa tggctctgtt gtctctttac aaatacttcg ctgtgttgat 60

gctggtgaac ggagtcgtca gctacaaatc cgctatgaac ttggaagcgt ttatggccca 120

cgactatttg ctgaacacca tgtatgaaga ttggctgaaa ataagcagtt atgcttataa 180

gagtggtaac gaagtatgtc aacagctact cggcgtagag ataaacaaga atgaaacaag 240

tgctgaaagg gagacacgcg aacagaacat gtttaattgt gttggtcgtt tcgtgtatcg 300

cgtaattccc acagcaagtc ttcctgggga agacccacat gatatagtgt tgcgcaaata 360

tggattggac ggtattcgga aagttatgga tcaaaaatat gcagacttct tcgaagagat 420

aattcagcga atggatgatt ttgtgtttgg tttaacgccg gcgcagcaaa gcgacacggc 480

agtgcaaaat ttgaaaggat ggttaagtaa tattaagagt gcctcgactt tggcggagaa 540

agaaatgact tttagaaaat gtatggaatt ttataaattt cagaggattt tttaatgtat 600

ctaaataaaa gtaaagatac tcgataataa attttctaaa atgcaagcaa agtcaagaaa 660

gaaaaaaaca 670

<210> 224

<211> 455

<212> DNA

<213> S003(10)-432

<400> 224

caagacgcaa atgaagaaaa tcttgttgaa ggtcgccatt ttaactgtga atgttcaatg 60

taattgtccc catgtacaat tcacatattc ttgaaaatat ttgcatttaa aaaatttctg 120

gaacatttaa taggcttgct atggtctctt ctgcctctga agaatcagaa cttgggtttg 180

ctgtaatttc ttctgcctct gaggaatcag aacttccttg ttcaagtgct gatgtaactt 240

ctgagtttcg cactaagcaa acctcaaatt tcacatattc accgtgtaca cttttgaaga 300

gttcttctgt gcggaagaat atttttgcat agtcaactgg ggaaaaatta ccggtgtgag 360

cctggaatgc ccggaccact ttttgcatcg ctgagaagta gcaatcattg ctttcctttt 420

gtgcttcgca gttgcttata tttgctaaga attcc 455

<210> 225

<211> 437

<212> DNA

<213> S003(10)-469

<400> 225

atggggagtt atcaaaagcg ttatcccaaa atatgcagca tacgttgtcc ctggagggaa 60

aattttcagc tgccatggaa gaaatcgcta aactccaacg gcaaaacgcg atgatgctag 120

atgtcatcaa taaaacctta tatagtaata gaaaaaaaaa aaaaaaaaaa aagtactgag 180

aaattgccga tttcgcatat cgcgaatgct gctaaagaac cccagacttg aatagcacaa 240

ggactggtaa ataatgggtg attaacgtgg taggaaacgt atgcggtgaa tccccagtag 300

tagctgcaat ttttgaacag attacgaatg ggcatagtgg catgcgagaa acgatgtacg 360

taatgatttc gttgttgaga atatataaat caccacgcca tttccacacc atgaaatcat 420

accgatgcag atcacgt 437

Claims

1. detect a cDNA chip for the anti-Trichlorphon of citrus fruit fly and effective cypermethrin Detection of insecticide resistance, it is characterized in that, comprise 221 target gene DNA probes; Wherein 118 target gene DNA probes are citrus fruit fly anti-Trichlorphon resistance relative specific gene fragment, and 103 target gene DNA probes are citrus fruit fly anti-effective cypermethrin resistance relative specific gene fragment;

Wherein, 118 described citrus fruit fly anti-Trichlorphon resistance relative specific gene fragments are: S004 (4)-A9, S004 (4)-B7, S004 (4)-D6, S004 (4)-H9, S004 (5)-B10, S004 (5)-B5, S004 (5)-C10, S004 (5)-C7, S004 (5)-D1, S004 (5)-D10, S004 (5)-D11, S004 (5)-G1, S004 (5)-H3, S004 (5)-H7, S004 (6)-10, S004 (6)-12, S004 (6)-18, S004 (6)-21, S004 (7)-100, S004 (7)-109, S004 (7)-138, S004 (7)-156, S004 (7)-165, S004 (7)-183, S004 (8)-197, S004 (8)-199, S004 (8)-217, S004 (8)-225, S004 (8)-240, S004 (8)-248, S004 (9)-293, S004 (9)-308, S004 (9)-328, S004 (10)-394, S004 (10)-401, S004 (10)-443, S004 (10)-471, S005 (1)-A1, S005 (1)-B2, S005 (1)-C1, S005 (1)-D12, S005 (1)-G8, S005 (2)-A8, S005 (2)-C7, S005 (2)-E8, S005 (2)-G1, S005 (3)-A5, S005 (3)-A9, S005 (3)-A11, S005 (3)-C11, S005 (3)-D12, S005 (3)-D8, S005 (3)-H12, S005 (4)-A2, S005 (4)-A3, S005 (4)-A8, S005 (4)-B8, S005 (4)-G1, S005 (4)-G2, S005 (5)-A5, S005 (5)-B7, S005 (5)-E11, S005 (5)-G6, S005 (5)-G9, S005 (5)-H2, S005 (6)-13, S005 (6)-17, S005 (6)-49, S005 (7)-98, S005 (7)-100, S005 (7)-103, S005 (7)-116, S005 (7)-107, S005 (7)-131, S005 (7)-176, S005 (7)-178, S005 (7)-181, S005 (7)-188, S005 (7)-189, S005 (8)-200, S005 (8)-194, S005 (8)-211, S005 (8)-212, S005 (8)-213, S005 (8)-216, S005 (8)-224, S005 (8)-235, S005 (8)-236, S005 (8)-254, S005 (8)-259, S005 (8)-261, S005 (8)-286, S005 (8)-264, S005 (9)-293, S005 (9)-299, S005 (9)-304, S005 (9)-308, S005 (9)-310, S005 (9)-312, S005 (9)-317, S005 (9)-329, S005 (9)-333, S005 (9)-350, S005 (9)-355, S005 (9)-357, S005 (9)-358, S005 (9)-359, S005 (9)-371, S005 (10)-388, S005 (10)-390, S005 (10)-401, S007 (3)-G11, S007 (6)-34, S007 (7)-107, S007 (7)-178, S007 (8)-192, S007 (8)-259, S007 (10)-420, sequence is as shown in SEQ ID NO.5 ~ SEQ ID NO.122,

103 described citrus fruit fly anti-effective cypermethrin resistance relative specific gene fragments are: S002 (1)-A3, S002 (1)-B4, S002 (3)-D9, S002 (3)-E7, S002 (4)-C10, S002 (4)-E4, S002 (4)-F8, S002 (5)-E5, S002 (6)-16, S002 (6)-19, S002 (6)-82, S002 (8)-270, S002 (10)-416, S002 (10)-452, S002 (10)-444, S008 (1)-B2, S008 (1)-D11, S008 (1)-D5, S008 (1)-D6, S008 (1)-E3, S008 (1)-F10, S008 (1)-F12, S008 (2)-B1, S008 (2)-F10, S008 (2)-F12, S008 (2)-G1, S008 (3)-B10, S008 (3)-C11, S008 (3)-D10, S008 (5)-B12, S008 (5)-B8, S008 (5)-C7, S008 (5)-E5, S008 (5)-G11, S008 (5)-H2, S008 (6)-14, S008 (6)-29, S008 (6)-42, S008 (6)-58, S008 (6)-64, S008 (6)-77, S008 (6)-85, S008 (6)-93, S008 (7)-130, S008 (7)-135, S008 (8)-276, S003 (1)-A10, S003 (1)-B6, S003 (1)-D7, S003 (1)-E1, S003 (1)-E9, S003 (1)-F2, S003 (1)-F5, S003 (1)-F9, S003 (1)-G4, S003 (2)-C6, S003 (2)-D4, S003 (2)-D5, S003 (2)-E7, S003 (2)-F3, S003 (2)-G5, S003 (3)-F1, S003 (3)-H10, S003 (4)-A8, S003 (4)-C1, S003 (4)-C12, S003 (4)-F1, S003 (4)-G6, S003 (5)-B11, S003 (5)-B2, S003 (5)-B8, S003 (5)-E6, S003 (5)-H5, S003 (6)-8, S003 (6)-32, S003 (6)-51, S003 (6)-74, S003 (6)-81, S003 (6)-92, S003 (7)-151, S003 (7)-160, S003 (7)-165, S003 (7)-167, S003 (7)-175, S003 (8)-196, S003 (8)-204, S003 (8)-220, S003 (8)-224, S003 (8)-231, S003 (8)-249, S003 (8)-271, S003 (8)-284, S003 (8)-288, S003 (9)-311, S003 (9)-312, S003 (9)-321, S003 (9)-352, S003 (10)-395, S003 (10)-404, S003 (10)-405, S003 (10)-406, S003 (10)-432, S003 (10)-469, sequence is as shown in SEQ ID NO.123 ~ SEQ ID NO.225.

2.the construction process of cDNA chip described in claim 1, is characterized in that, step is as follows:

S1. Using Suppression Subtractive Hybridization builds the anti-Trichlorphon of citrus fruit fly and anti-effective cypermethrin resistant strain SSH library respectively;

S2. difference random choose difference loss of weight group clone from two libraries that S1 builds, extracts recombinant plasmid dna;

S3. respectively with S2 obtain recombinant plasmid dna for template, with the 2R of primer Nested PCR Primer shown in primer Nested PCR Primer 1F and SEQ ID NO.2 shown in SEQ ID NO.1 for adapter-primer carries out pcr amplification, detect the unicity of each PCR primer concentration and fragment, purifying reclaims and obtains product;

S4. purifying is reclaimed the product point sample obtained on the carrier of chemically modified, the positive and negative control are set simultaneously, obtain initial gene chip;

The resistant strain total serum IgE used when S5. adopting step S1 to build library respectively and sensitive strain total serum IgE are for detecting sample, carry out DNA chip hybridization, remove false positive differential gene, the sample selecting hybridization rear rise signal value on gene chip to be greater than 2 is resistance relevant difference expressing gene fragment;

The recombinant plasmid of the resistance relevant difference expressing gene fragment S6. obtained with S5 carries out pcr amplification for template, primer is with described in S3, carry out purifying recovery, order-checking, splicing assembling to product, remove and repeat and miscellaneous sequence, picking goes out single non-overlapping sequence as resistance specified target gene;

S7. the resistance specified target gene point sample obtained by S6, on the carrier of chemically modified, arranges the positive and negative control simultaneously, builds the cDNA chip obtaining the resistance level detecting the anti-Trichlorphon of citrus fruit fly and effective cypermethrin.

3.according to claim 2, the construction process of cDNA chip, is characterized in that, the carrier described in S4 and S7 is slide glass material.

4.the construction process of cDNA chip according to claim 2, it is characterized in that, chemically modified described in S7 refers to introduces amido functional group by chemical reaction on carrier surface, enables DNA and surface functional group energy covalent attachment, stably solidifies in support surface.