CN103601821B

CN103601821B - A kind of enzymolysis processing method and corresponding heparin sodium working method

Info

Publication number: CN103601821B
Application number: CN201310568767.XA
Authority: CN
Inventors: 刘建召; 黄刚; 高明国; 刘洋
Original assignee: CHONGQING SANTENG FOOD Co Ltd
Current assignee: CHONGQING SANTENG FOOD Co Ltd
Priority date: 2013-11-15
Filing date: 2013-11-15
Publication date: 2016-01-06
Anticipated expiration: 2033-11-15
Also published as: CN103601821A

Abstract

The invention discloses the heparin sodium working method of a kind of enzymolysis processing method and correspondence, described heparin sodium working method comprises the large process of postcibal diarrhea, enzymolysis, absorption, wash-out and precipitation five, its feature to be in postcibal diarrhea operation to collect mucus respectively twice and washing fluid is intestinal mucosa liquid, carries out three grades of wash-outs in elution procedure.Drastically increase heparin sodium extraction efficiency.Wherein namely enzymolysis operation have employed enzymolysis processing method of the present invention, adopts the enzymolysis device with automatic detection and control system to carry out enzymolysis processing, drastically increases enzymolysis atomization degree and degree of reliability.

Description

A kind of enzymolysis processing method and corresponding heparin sodium working method

Technical field

The present invention relates to heparin sodium production technique field, specifically relate to the heparin sodium working method of a kind of enzymolysis processing method and correspondence.

Background technology

Heparin sodium is a kind of mucopolysaccharidesulfuric acid ester anticoagulant is the intestines by pig or ox mucous membranethe sodium salt of the CSSO3 of middle extraction, belongs to mucopolysaccharide material, is also the most complicated compound of molecular structure known up to now in the world, in a short time cannot synthetic, at present can only from the mucous membrane of small intestine of the livestock such as pig or ox extraction and isolation.Recent study proves that heparin sodium also has reducing blood lipid, has very high pharmaceutical use.

The heparin stoste obtained from the mucous membrane of small intestine of live pig, due to containing impurity such as a large amount of albumen, nucleic acid, microorganisms, through physics and chemistry extraction and isolation process, need could obtain the heparin sodium crude that natural structure group is complete, thus make heparin bulk drug.In the existing heparin sodium working method of heparin first product, generally can need through process such as postcibal diarrhea, enzymolysis, absorption, wash-out and precipitations.

But the preparation method of existing heparin sodium first product, exists following defect: 1, heparin sodium extraction efficiency is not high, every 1600-1700 root chitterlings could prepare the heparin sodium first product of one hundred million unit (referring to actives prime number) substantially.2, adopt manual operations in each step, level of automation is low more, causes efficiency low.

More particularly, at present, the artificial postcibal diarrhea of general employing in postcibal diarrhea operation, efficiency is very low.In order to raise the efficiency, in prior art, the invention of CN201388473Y was once disclosed a kind of archenteron-scrapping machine, its structure comprises frame, the bottom of frame is provided with motor, speed reduction unit, speed reduction unit is provided with two output shafts and thong wheel or sprocket wheel, wherein has left and right supporting base in the upper design of frame, is provided with active rubber roll, transition rubber roller between two supporting bases successively, and doctor roll, and three rollers are low early and high after being in tilted layout; Wherein active rubber roll and doctor roll separately one end shaft extension go out supporting base, axle is installed belt pulley or sprocket wheel, is connected with the belt pulley on reducer output shaft or chain sprocket drive respectively by belt or chain; It is large that this device can solve artificial postcibal diarrhea labour intensity, and production efficiency is low, the problem that yield rate is low.But still there is following defect.

1, the postcibal diarrhea mechanism of this device is primarily of active rubber roll, transition rubber roller, and doctor roll is formed, hair intestines carry out striking between doctor roll and transition rubber roller, hair intestines walk around again transition rubber roller surface afterwards through the gap transition rubber roller and active rubber roll simultaneously, draw by the rotation between active rubber roll and transition rubber roller, the pulling speed of such hair intestines and striking speed all can be affected by the velocity of rotation of transition rubber roller, cause being difficult to grasp the balance between the traction of hair intestines and striking process, and then mao intestines are broken out or to be difficult to striking clean.Therefore the defect that the existence of this device is difficult to regulate postcibal diarrhea speed and makes postcibal diarrhea effect poor.

2, this device only can realize a mao function for intestines striking, cannot realize mao intestines and just process, and the step such as subsequent disposal after striking, and be difficult to the production line balance realizing postcibal diarrhea technique, production efficiency is lower.

In enzymolysis operation, the general enzymatic vessel that uses carries out enzymolysis.The patent of CN200920084667.9 was once disclosed a kind of enzymatic vessel, and its structure includes tank body, and tank body upper end is provided with driving motor, motor is connected with agitator arm, agitator arm stretches in tank body, and inner tank wall is provided with heating coil, and the entrance end of heating coil is connected with the heat source tube outside tank body, tank body top is provided with opening for feed, spy hole and aproll ball, tank body lower end is provided with tail gas mouth, and lower end is provided with discharge gate, enzymatic vessel, described agitator arm is equipped with paddle, can fully stirs.The enzymatic vessel of this invention has high insulating effect, dissolves fully, the feature do not polluted; But still there is following defect.

1, be only provided with an opening for feed in this enzymatic vessel, be unfavorable for some interpolations adjusted.

2, this enzymatic vessel agitator arm is directly connected with motor, and agitator arm is stressed comparatively large when stirring, and can produce very large shock and vibration, easily cause fracture.

3, manhole does not arrange manhole plate, heat insulation effect poor and easily cause stir time liquid spill.

4, during enzymolysis, the controling parameters such as pH value and salinity needs manually to carry out detections rule of thumb carry out red-tape operati by workman, there is inefficiency and reliability difference empirically causes hydrolysis result to be difficult to the defect of guarantee.

Elution procedure utilizes sodium chloride solution to carry out wash-out to the resin being adsorbed with heparin compositions in absorption process, makes containing heparin sodium in elutriant, and elutriant and resin isolation are come by corresponding device.In prior art, publication number CN202526939U's patent discloses a kind of resin elution tank, which employs following technical scheme: described wash-out tank is provided with feed port, discharge hole, inlet opening, liquid outlet and temp probe, it is characterized in that described wash-out tank is Double-layer clamp nested structure, comprise inner barrel and heat exchange outer sleeve body, heat exchange outer sleeve body is hollow structure, inside be provided with and lead warm medium, the laminating of heat exchange outer sleeve body surrounds on the outer side of inner barrel, resin isolation screen cloth is provided with in described wash-out tank, resin isolation screen slope is arranged, its lower one end and discharge hole are in sustained height.The wash-out functions of the equipments which solves the production of existing heparin are simple, and process control bothers, the problem of the easy fast erosion of equipment and materials, but still there is following defect:

1, this equipment wash-out resin is utilized once, the extraction of heparin can be caused insufficient, not only reduce the unit output of heparin sodium crude, also have impact on adsorption effect when resin reuses, reduce the production efficiency of heparin sodium, decrease the economic benefit of producing heparin sodium.

2, this equipment is utilized to carry out repeatedly wash-out to resin, also need just can complete (container that is used as savings elutriant or resin as increased) in conjunction with other instrument, moreover, a large amount of artificial participation is also needed in operating process, so, not only complex operation bothers, improved efficiency is not obvious, also can increase production cost.

In a word, in the heparin sodium working method of prior art, adopt said apparatus to operate at described postcibal diarrhea, enzymolysis, elution procedure, the extraction that there is heparin is insufficient, the problem that heparin sodium production efficiency is lower.

Summary of the invention

For the problems referred to above and deficiency, technical problem to be solved by this invention is: how to provide the enzymolysis processing method that a kind of level of automation is high, a kind of heparin sodium working method that have employed this enzymolysis processing method is also provided simultaneously, makes it improve heparin sodium extraction efficiency and productivity effect.

In order to solve the problem, present invention employs following technical scheme:

A kind of enzymolysis processing method, is characterized in that, adopts enzymatic vessel to carry out enzymolysis; First, the intestinal mucosa liquid feed valve on enzymatic vessel is opened; Then, the fresh intestinal mucosa liquid obtained in postcibal diarrhea operation is sent in enzymatic vessel, and intestinal mucosa liquid in enzymatic vessel is stirred; , intestinal mucosa liquid in enzymatic vessel is heated meanwhile, in intestinal mucosa liquid, progressively add alkali and the mixed liquid of synthesis converted by salt simultaneously, when heating to 47 ~ 53 DEG C, adding Sumizyme MP by mixed liquid enzymolysis processing is enzymolysis solution, controlled enzymatic hydrolysis liquid PH=8 ~ 9, and salinity is 3 ~ 3.5 degree; Be incubated 2 ~ 3 hours, be then warming up to 80 ± 5 DEG C, and insulation is no less than 30 minutes; Finally, stop stirring enzymolysis solution in enzymatic vessel, open enzymatic vessel baiting valve and enzymolysis solution is put into hay tank (with 80 ~ 100 mesh filter screens) filtration.

Aforesaid method specifically, the heparin sodium first obtaining following structure produces enzymolysis device, described heparin sodium is produced enzymolysis device and is comprised enzymatic vessel, the tank body top of described enzymatic vessel is provided with manhole, lower end is provided with discharge port, enzymatic vessel is also provided with whipping appts and heating unit, it is characterized in that, described tank body is also provided with the intestinal mucosa liquid liquid-inlet pipe, alkali lye liquid-inlet pipe, salt solution water inlet pipe and the tap water water inlet pipe that are communicated with tank inner chamber, described heparin sodium is produced enzymolysis device and is also comprised automatic detection and control system, described automatic detection and control system comprises the temperature sensor for detecting temperature of charge be arranged in inner tank wall, for detecting the pH value detecting sensor of material potential of hydrogen and the salometer for detecting material salinity, described temperature sensor, pH value detecting sensor is connected with salometer all with control center and provides detection data for it, described automatic detection and control system also comprises and is separately positioned on described intestinal mucosa liquid liquid-inlet pipe, alkali lye liquid-inlet pipe, electric-controlled switch valve on salt solution water inlet pipe and tap water water inlet pipe, described intestinal mucosa liquid liquid-inlet pipe, alkali lye liquid-inlet pipe, salt solution water inlet pipe and the electric-controlled switch valve on tap water water inlet pipe are all connected with described control center separately and control by it,

Described heating unit comprises the heating coil arranged along the coiling of enzymatic vessel inner tank wall, heating coil has the inlet pipe of extending tank body from upper end, there is the escape pipe extending tank body from lower end, described inlet pipe is provided with electrical control steam valve, described electrical control steam valve is connected with described control center and controls by it;

Described whipping appts comprises the agitator motor being positioned at tank body top, vertically be arranged on the stir shaft in the middle part of tank inner chamber, by stir shaft, supporting structure is installed between described agitator motor and described stir shaft to be connected, described stir shaft installs supporting structure, comprise and be upwards fixed on tank body top and overall tubular supporting base, there is in the middle part of supporting base a horizontally disposed brace table, described agitator motor is fixed on supporting base top and electric machine main shaft extends down in supporting base, brace table in the middle part of described supporting base is rotatably mounted with a tunnel shaft by bearing, end, tunnel shaft upper end is docked and transmitting torque by concave-convex fit structure with agitator motor output shaft lower end, described tunnel shaft lower end adjacent end position permanent sleeve is provided with on a circle and connects steel bushing, described stir shaft upper end extends in supporting base and permanent sleeve is provided with one and lower connection steel bushing corresponding to upper connection steel bushing, several cylinder lever connecting rods of annular uniform setting between described upper connection steel bushing and lower connection steel bushing, cylinder lever connecting rod two ends adopt nut to be connected through described upper connection steel bushing with lower connection steel bushing, between upper connection steel bushing and lower connection steel bushing, also annular is evenly equipped with several whisker simultaneously, described helical spring upper and lower two ends are fixed on connection steel bushing and lower connection steel bushing respectively,

On described upper connection steel bushing, annular is furnished with several joint bolt, described joint bolt front end edge connects steel bushing outer side level and penetrates in tunnel shaft the connection realizing upper connection steel bushing and tunnel shaft;

On described tank body, manhole place is also provided with manhole cover structure, described manhole cover structure to comprise along manhole to tank body the outside circle eck that convexes to form and surround cover type and be arranged on lid on eck of stretching, lid upper surface is diametrically fixedly installed a mounting rod, one end and the free bearing be fixed on eck of mounting rod are rotatably connected, the other end of mounting rod extends lid and is provided with the U-lag that a horizontal section takes the shape of the letter U, eck corresponding below U-lag is provided with a locking lever rotationally, locking lever outer end is also connected with a handles by screw thread activity, locking lever outer end upwards can be rotated and entered in described U-lag and be locked at U-lag upper surface by rotating handles,

Then operate according to following operation steps:

First, the electric-controlled switch valve charging of opening intestinal mucosa liquid liquid-inlet pipe by control center, pours into intestinal mucosa liquid in enzymatic vessel;

Secondly, start the agitator motor at tank body top, stir shaft starts to stir; Meanwhile, the data of control center's automatic detection for temperature sensor, pH value detecting sensor and salometer, and control the electric-controlled switch valve on electrical control steam valve, alkali lye liquid-inlet pipe and the electric-controlled switch valve on salt solution water inlet pipe respectively, such that the temperature of enzymolysis solution in tank maintains 47 ~ 53 DEG C, pH value maintains PH=8 ~ 9, salt angle value maintains 3 ~ 3.5 degree;

Finally, after axle to be mixed stirs 2.5 hours (within 2 to 3 hours, can both realize abundant stirring), control center controls electrical control steam valve and will be warming up in tank 80 DEG C (all suitable within the scope of 80 ± 5 DEG C), and maintenance is no less than 30 minutes; Subsequently, close the agitator motor at tank body top, stir shaft stops stirring; Open enzymatic vessel lower end and be provided with discharge port, enzymolysis solution is delivered to hay tank.

In above-mentioned enzymolysis processing method, in the enzymolysis device adopted, tank body is also provided with the intestinal mucosa liquid liquid-inlet pipe, alkali lye liquid-inlet pipe, salt solution water inlet pipe and the tap water water inlet pipe that are communicated with tank inner chamber, like this, can the control realization intestinal mucosa liquid charging of tube road, the interpolation of the interpolation and tap water that conveniently realize alkali lye, with adjusted to ph, conveniently realizes the interpolation of salt solution and the interpolation of tap water to adjust salinity.Namely the interpolation of adjustment is facilitated.Simultaneously, in the present invention, also be provided with automatic detection and control system, make it operationally, each sensing detection device can be leaned on to detect each parameter situation, and after then detection data being sent to control center, control center can according to pre-set programs by the adjustment to each electric-controlled switch valve, control to adjust with addition of realizing control to each parameter, make it meet production control demand.So just achieve automated control operation, avoid the inefficiency existed by manual detection, reliability difference and the high defect of cost of labor, ensure that enzymolysis process carries out under being in the working condition accurately controlled, ensure that hydrolysis result.

Described heating unit comprises the heating coil arranged along the coiling of enzymatic vessel inner tank wall, heating coil has the inlet pipe of extending tank body from upper end, there is the escape pipe extending tank body from lower end, described inlet pipe is provided with electrical control steam valve, described electrical control steam valve is connected with described control center and controls by it.Like this, during production, inlet pipe can be connected with production of steam system with escape pipe, control steam enters to add in coil pipe and realizes heating and insulation, control the switch of electrical control steam valve by control center according to the temperature data that temperature sensor detects, realize the control of temperature.Have structure simple, it is convenient to implement, and is easy to the advantages such as control.

When enzymatic vessel whipping appts works, described agitator motor output shaft drives tunnel shaft to rotate by concave-convex fit structure, tunnel shaft realizes the function of shaft coupling and bumper and absorbing shock, specifically tunnel shaft lower end drives stir shaft to rotate by the cylinder lever connecting rod that arranges between upper connection steel bushing and lower connection steel bushing and whisker, like this, the helical spring elastic force arranged is converted to circumferential component by pull bar continuous curve when transmission spin load, it is made to possess higher turning shock loads surge capability, reach the effect of protection shaft coupling part, agitator arm is avoided to rupture because shock load is comparatively large, extend whipping appts work-ing life.Wherein, the upper spacing connecting steel bushing and lower connection steel bushing can be regulated by regulating the nut at cylinder lever connecting rod two ends, realizing the adjustment to helical spring deformation degree, and then realizing the fine setting to device surge capability simultaneously.

On described upper connection steel bushing, annular is furnished with several joint bolt, described joint bolt front end edge connects steel bushing outer side level and penetrates in tunnel shaft the connection realizing upper connection steel bushing and tunnel shaft.Like this, the connection between connection steel bushing and tunnel shaft and dismounting can conveniently be realized.

On described tank body, manhole place is also provided with manhole cover structure, and manhole plate can be leaned on to realize the opening and closing of manhole quickly and easily, and the foul smell produced during to avoid producing overflows.Specifically, locking lever is upwards screwed in U-lag after closing by manhole plate, then is screwed by rotating handles and be close to U-lag upper surface, locking can be realized, when open, only need oppositely rotating handles to be outwarded winding, again locking lever is put down, can manhole plate be opened, fast very convenient.Wherein, described lid comprises the flange of a steel, and is embedded in the toughened glass of flange inside.Like this, lid bulk strength can be ensured, be beneficial to again and observe tank interior situation by lid.One corresponding groove can also be set in lid inside edge and eck contact position further and in groove, be embedded a circle elastic seal ring, like this can so that realize sealing, during to avoid producing, foul smell leaks.In addition, when specifically implementing, tank body can also be communicated with a vapor pipe is set, to realize the outer row of process gas, avoid the closedown because of manhole plate produce high pressure and affect production simultaneously.

In sum, this enzymolysis processing method can realize the Automated condtrol of enzymolysis, guarantees hydrolysis result and improves production efficiency.

Present invention also offers a kind of a kind of heparin sodium working method that have employed above-mentioned enzymolysis processing method, comprise the large process of postcibal diarrhea, enzymolysis, absorption, wash-out and precipitation five, it is characterized in that, in described postcibal diarrhea operation, the mucus scraped in casing is collected when carrying out striking to hair intestines and making its intestines skin and casing be broken, again poured water in casing inside after intestines skin is separated simultaneously and rinse and collect washing fluid, using described mucus and washing fluid all as the intestinal mucosa liquid in described enzymolysis operation;

In described elution procedure, adopt salt solution to carry out three grades of wash-outs to the resin leached from described absorption process, wherein third stage wash-out adopt new preparation salt solution (becoming elutriant after wash-out) and from the resin leached after the wash-out of the second stage, second stage wash-out adopts from the resin leached after the elutriant after third stage wash-out and first step wash-out, first step wash-out adopts from the elutriant after the wash-out of the second stage with from the resin leached in described absorption process, and the elutriant after first step wash-out enters follow-up described precipitation operation.Wherein, when carrying out three grades of wash-outs in described elution procedure, third stage elution time is 1 hour, and second stage elution time is 1.5 hours, and first step elution time is 3 hours.Namely described enzymolysis operation adopts above-mentioned enzymolysis processing method to carry out.

Heparin sodium working method of the present invention, by in postcibal diarrhea operation, be collected in and the mucus that scrapes in casing when striking makes its intestines skin and casing be broken is carried out to hair intestines, with the washing fluid of flushing of again pouring water to casing inside after intestines skin is separated, significantly reduce and be rich in the mucus of heparin compositions and the loss of washing fluid in postcibal diarrhea operation, using this mucus and washing fluid as the intestinal mucosa liquid in enzymolysis operation, thus increase the extraction efficiency of heparin sodium.In elution procedure, adopt salt solution to carry out three grades of wash-outs to the resin that absorption process leaches, while abundant wash-out resin, (resin needs Reusability, after abundant wash-out, when next time is for adsorbing, adsorption effect is better), also more heparin sodium can be extracted, increase the output of the heparin sodium of unit raw material, promote the productivity effect of producing heparin sodium.Adopt three grades of wash-outs, like this, the resin of third stage wash-out is stripped the resin of twice, and elutriant is wherein the salt solution of new preparation, therefore designs the minimum time, and one hour enough elutes effective constituent remaining in resin; The first step is fresh resin wash-out, and utilization has stripped twice elutriant, therefore designs the longest elution time to make to contain more active substance in the elutriant of arrival post precipitation operation.According to the elution time of above-mentioned setting wash-out not at the same level, better elute effect can not only be played, unnecessary elution time can also be reduced, reduce wash-out energy consumption, further increase the productivity effect of heparin sodium.

As concrete optimization process in aforesaid method,

In described absorption process, first, described enzymolysis solution is sucked adsorption tanks, described enzymolysis solution is stirred, when described enzymolysis solution is cooled to 54 DEG C ~ 59 DEG C, add heparin adsorption resin (AMBERLITEFPA98CL resin), insulated and stirred absorption 8 ~ 10 hours;

In described elution procedure, polymeric adsorbent described in described absorption process is rinsed well, put into wash-out tank after being filtered dry and carry out wash-out, add the salt solution (sodium chloride solution identical with weight resin again, salinity is 19 ~ 23 degree Beaume) convert synthesis batch mixing, described batch mixing is heated to 55 ~ 60 DEG C, carries out wash-out;

In described precipitation operation, use the elutriant from elution procedure, add the alcohol of concentration 80% ~ 90%, after stirring, alcoholic strength controls at 35 ~ 45 degree, precipitates 20 ~ 24 hours, extracts supernatant liquor out, collecting precipitation thing is treated to be placed in dehydration barrel in 3 ~ 5 days, add the alcohol that concentration is 90% ~ 95%, alcohol is about 2:1 with sediment volume ratio, dewaters 1 ~ 2 hour, extract supernatant liquor out, collect residue heparin sodium dry.

Described like this absorption process, makes polymeric adsorbent adsorb heparin compositions in enzymolysis solution more fully.Adsorb the resin of heparin compositions in described elution procedure described in wash-out described in absorption process, obtain elutriant and extract heparin sodium for precipitating in operation.The alcohol-pickled flushing of twice employing in described precipitation operation, improves heparin sodium precision.

As improvement, in described postcibal diarrhea operation, first obtain the heparin sodium processing postcibal diarrhea device of following structure, described heparin sodium processing postcibal diarrhea device comprises frame and is arranged on the archenteron-scrapping machine in frame, described archenteron-scrapping machine comprises the left and right supporting base being fixed on the left and right sides in frame, with the striking mechanism be horizontally installed on the supporting base of left and right and haulage gear, described striking mechanism comprises the doctor roll and auxiliary rubber roller that are set up in parallel, it is characterized in that, haulage gear in described archenteron-scrapping machine, comprise a pair take off roll be set up in parallel, take off roll length direction is consistent with doctor roll length direction and be positioned at the position, front lower place in doctor roll and auxiliary rubber roller gap, the termination of a pair take off roll is provided with and engages each other and a pair gear for driving two take off roll relatively to rotate,

In described archenteron-scrapping machine, also comprise position-limit mechanism, described position-limit mechanism comprise transverse strands to be located on the supporting base of left and right and between haulage gear and striking mechanism the support bar of position, on support bar, forward upward is set side by side with the limited post of several uniform intervals distribution;

Also be provided with in described archenteron-scrapping machine for by doctor roll the doctor roll be covered under it renovate, the two ends that described doctor roll renovates front side edge are hinged on the supporting base of left and right rotationally;

Described doctor roll renovates the viewing window just doctor roll top position being also provided with to glass material;

Described heparin sodium processing postcibal diarrhea device also comprises the worktable of long strip shape, described archenteron-scrapping machine is positioned at worktable middle part, the table top of described worktable rear end section start is provided with primary launder, primary launder Inner Front End position is each side provided with a water faucet for previous cleaning, and in primary launder, rear end is provided with sewage outlet; Also be connected between primary launder with archenteron-scrapping machine and be provided with receiving tank, receiving tank rear end has one for collecting the mucous membrane pond of scraping intestinal mucosa; Worktable is positioned at archenteron-scrapping machine anterior position and is also provided with water flowing cleaning leak detection groove, described water flowing cleaning leak detection groove Inner Front End position is each side provided with a water faucet for water flowing leak detection, and in water flowing cleaning leak detection groove, rear end is also provided with leak detection liquid collecting tank;

Be positioned at water faucet rear position in described primary launder and water flowing cleaning leak detection groove to be provided with one and to lose small opening, lose activity in small opening and be linked with one and lose leaky bucket;

Then operate according to following operation steps:

First, first the foreign material such as the loose fat of hair intestines surface attachment are extractd in primary launder, and the foreign material of excision are directly discarded in leaky bucket by losing small opening; To be poured water flushing for the water faucet of previous cleaning to connecting hair intestines one end, the waste water of flushing is arranged outward by sewage outlet again; After rinsing, hair intestines deliver to receiving tank forward, hair intestines are put along worktable length direction by receiving tank and after being many along worktable width laid out in parallel on machine postcibal diarrhea;

Subsequently, when postcibal diarrhea, many hair intestines go up archenteron-scrapping machine side by side, and adjacent hair intestines spacing 15-25cm also leans on the limited post of position-limit mechanism to separate; Start archenteron-scrapping machine, hair intestines termination is sent between the doctor roll of striking mechanism and auxiliary rubber roller and pass, forward downward is sent between a pair take off roll of haulage gear again, a pair take off roll are clamped mao intestines and are relatively rotated and provide tractive force automatically to pull mao intestines to carry out striking in striking mechanism, scrape the mucous membrane liquid made and flow to mucous membrane pond by receiving tank and regather;

Finally, hair intestines after striking deliver to forward water flowing cleaning leak detection groove, by manually the intestines skin of casing surface attachment being removed and putting into the basket of specifying, again remaining casing is adopted water faucet water flowing cleaning, find during cleaning that the casing of leak or fragmentation need cut off, the water liquid cleaned out regathers after being pooled to leak detection liquid collecting tank.

Like this, described heparin sodium processing postcibal diarrhea device, during its work, hair intestines pass between the doctor roll and auxiliary rubber roller of striking mechanism, doctor roll and auxiliary rubber roller are rotated by driven by motor and carry out striking to hair intestines, then hair intestines pull out forward backward downward-extension from doctor roll and auxiliary rubber roller gap and are sandwiched between a pair take off roll, two take off roll are relatively rotated by driven by motor under the engagement of termination gear, for pulling of hair intestines provides traction.Like this, the rotation of take off roll and the rotation of doctor roll are just independent separately, can not influence each other, and convenient regulating respectively separately pulling speed and striking speed, makes it reach best striking effect.During due to striking, be generally the strikings together of several maos of intestines, after setting up position-limit mechanism, each hair intestines can separate by the support bar in position-limit mechanism, avoid contacting with each other between mao intestines and produce the phenomenons such as winding or blocking.When striking, doctor roll is renovated and cover, mao liquid of intestines surface attachment can be avoided to spill when striking, when needs are cleared up, doctor roll is renovated simultaneously and open cleaning, can conveniently the dirt settling on doctor roll surface be cleaned out.Described doctor roll renovates the viewing window just doctor roll top position being also provided with to glass material, whether normal can observe doctor roll work conveniently by viewing window.

Described postcibal diarrhea device is provided with worktable, can realize the pipelining of whole postcibal diarrhea technique.Specifically, operationally, the loose fat of hair intestines surface attachment is first extractd by workman in primary launder, again hair intestines one end docking upper hose tap is carried out flushing of pouring water, wash ight soil and parasite off, find during flushing that place that hair intestines have breakage to leak will be excised and the hair intestines length sent before guaranteeing is greater than two meters, the waste water of flushing is arranged outward by sewage outlet.After rinsing, hair intestines deliver to receiving tank forward, hair intestines are put along worktable length direction by receiving tank and after being many along worktable width laid out in parallel on machine postcibal diarrhea, during postcibal diarrhea, many hair intestines go up machine side by side, adjacent hair intestines spacing 15-25cm also leans on the limited post of position-limit mechanism to separate, archenteron-scrapping machine is started during upper machine, hair intestines termination is sent between the doctor roll of striking mechanism and auxiliary rubber roller and pass, forward downward is sent between a pair take off roll of haulage gear again, two take off roll are clamped mao intestines and are relatively rotated and provide tractive force automatically to pull mao intestines to carry out striking in striking mechanism, scrape the mucous membrane liquid made to flow to mucous membrane pond by receiving tank and regather as heparin stoste.Water flowing cleaning leak detection groove is delivered to before hair intestines after striking, by workman the intestines skin of casing surface attachment removed and put into the basket of specifying, again remaining casing is adopted water faucet water flowing cleaning, find during cleaning that leak or fragmentation need be cut off, the water liquid cleaned out regathers as heparin stoste after being pooled to leak detection liquid collecting tank.In whole process, the ratio that water consumption expends 6-7kg according to complete chitterlings controls.During such work, first extract loose fat loose fat can be avoided to enter heparin stoste after turn to when follow-up enzymolysis processing fluid float on liquid level affect enzymolysis operate; Find that there is the place that breakage leaks when rinsing hair intestines will excise, the smooth and easy of striking can be guaranteed, avoid running into damaged place when striking and blocking is shut down, hair intestines length guarantee two meters and striking time first arrange on receiving tank along worktable length direction, can guarantee that striking is carried out more smoothly, improve striking efficiency, during postcibal diarrhea, adjacent hair intestines separate by the limited post of position-limit mechanism and keep spacing 15-25cm, the adjacent hair intestines when striking can be avoided to collide and and cause be wound around or blocking, guarantee the smooth and easy of striking; After striking, water flowing leak detection again, guarantees that obtaining complete casing is beneficial to the processing of follow-up casing, the rinse water liquid of the leak detection of water flowing is simultaneously collected as heparin stoste again, so just farthest be extracted the heparin raw material in mao intestines, avoid waste, improve heparin sodium extraction efficiency.Water consumption control is the ratio of a small intestine 6-7kg, namely can guarantee the demand of cleaning and the acquisition of heparin, again saves and avoids waste with water.

The waste of excision of hunting leak in primary launder and water flowing cleaning leak detection groove directly can be discarded in leaky bucket and focuses on, guarantees the clean of job site and saves the time, improve the working efficiency of streamline.

As improvement, in described elution procedure, the heparin sodium first obtaining following structure produces washing device, described heparin sodium is produced washing device and is comprised wash-out tank, each wash-out tank lower end is provided with liquid discharge pipe, in described liquid discharge pipe, cross-sectional direction is provided with filter screen, and described liquid discharge pipe is provided with the switch-valve for controlling discharge opeing lower than described filter screen, also being provided with bypass discharge nozzle higher than described filter screen, described bypass discharge nozzle being provided with the switch-valve for controlling discharging; Described wash-out tank is at least two and is concatenated into multilevel hierarchy; In described multilevel hierarchy, the described liquid discharge pipe of next stage wash-out tank is connected by the upper end of Drainage pipe with upper level wash-out tank, described Drainage pipe is provided with the electric pump for discharge opeing; The described bypass discharge nozzle of upper level wash-out tank is connected by the upper end of discharge pipe with next stage wash-out tank, described discharge pipe is provided with the electric pump for discharging;

Described multilevel hierarchy is tertiary structure, and described tertiary structure comprises first step wash-out tank, second stage wash-out tank and third stage wash-out tank; The described liquid discharge pipe of described third stage wash-out tank is connected by the upper end of Drainage pipe with described second stage wash-out tank, and the described liquid discharge pipe of described second stage wash-out tank is connected by the upper end of Drainage pipe with described first step wash-out tank; The described bypass discharge nozzle of described first step wash-out tank is connected by the upper end of discharge pipe with described second stage wash-out tank, and the described bypass discharge nozzle of described second stage wash-out tank is connected by the upper end of discharge pipe with described third stage wash-out tank;

Described wash-out tank is also provided with stirring mechanism;

Described stirring mechanism comprises agitator motor and agitator arm, and described agitator motor is arranged on wash-out tank upper end, and described agitator arm stretches in wash-out tank, and described agitator motor connects with described agitator arm;

Also comprise frame in described multilevel hierarchy, described wash-out tank is arranged in described frame, and described frame is also provided with helper;

Described wash-out tank upper end is provided with manhole;

Described wash-out tank is also provided with heating arrangements;

Then operate according to following operation steps:

When heparin sodium in present embodiment produces washing device use, circulation performs following steps:

Step one: unlatching first step wash-out tank and third stage wash-out tank carry out wash-out simultaneously.Wherein, the resin that the resin of first step wash-out tank leaches from described absorption process, elutriant filters the elutriant obtained from last time circulation after the wash-out tank wash-out of the second stage; The resin of third stage wash-out tank filters the resin obtained from last time circulation after the wash-out tank wash-out of the second stage, and elutriant is from the fresh salt solution added; During wash-out, first step wash-out tank wash-out three hours, third stage wash-out tank wash-out 1 hour.

Step 2: first step wash-out tank in step one is stripped the elutriant leached and drains into the process of described precipitation operation, the resin leached drains into second stage wash-out tank; Third stage wash-out tank in step one is stripped leach elutriant drain into second stage wash-out tank, the resin leached collects recycling after arranging outward;

Step 3: open second stage wash-out tank and carry out wash-out, elution time 1.5 hours, after wash-out, the elutriant filtered out is drained into first step wash-out tank, resin drains into third stage wash-out tank, circulates since then;

In above-mentioned three steps, described wash-out tank, when wash-out, is opened in agitator motor 14 pairs of tanks and is stirred, while stirring, open heating arrangements, mixed solution in tank body is heated to 55 ~ 60 DEG C.

Like this, described heparin sodium is produced washing device and is adopted tertiary structure, heparin sodium elution processes can be made more excellent, namely same batch of resin treating wash-out can by wash-out three times in this washing device, sodium chloride solution in third stage wash-out tank also can in this washing device wash-out three times, thus make sodium chloride solution can extract the heparin compositions adsorbed in resin fully, and obtain the higher elutriant of heparin sodium content, simultaneously, be fully extracted the resin after heparin compositions, again can utilize in heparin sodium is produced, play better adsorption effect.In addition, the setting of tertiary structure, makes the simply easy to operate of this device, effectively can also save the time of the wash-out of heparin sodium, improve production efficiency.Moreover, compare with the structure being greater than three grades, obviously can also save energy consumption.

The stirring mechanism that wash-out tank is arranged, avoids the problem of hand mixing inefficiency, also makes elution processes can stir more abundant.

As the improvement of described multilevel hierarchy, also comprise frame in this mechanism, described wash-out tank is arranged in described frame, and described frame is also provided with helper.Like this, the arranging of frame can break even device can utilize longitudinal space, save floor space, reduce cost.

Wash-out tank upper end is provided with manhole, by manhole can be convenient add materials, also can observe the condition of production in tank very easily.

Described wash-out tank is also provided with heating arrangements.Like this, heating coil can be arranged in tank body directly to heat in tank body.Also can by tank body as double layer jacket structure, be a cavity between described chuck, be provided with heating coil in cavity, heating coil entrance end is connected with the heat source tube outside tank body, adopts the heating to tank body, indirectly to the technical scheme heated in tank body.Because elution process can reach better elute effect at a suitable temperature, so the setting of heating arrangement can help washing device of the present invention, get better elute effect.

In sum, the heparin sodium working method that the present invention relates to, compared with the existing technology comparatively, can improve the working efficiency that heparin is produced, and extracts the heparin compositions in hair intestines more fully, obtains the productivity effect of better heparin sodium.

Accompanying drawing explanation

Fig. 1 is the structural representation of the heparin sodium processing postcibal diarrhea device adopted in postcibal diarrhea operation in heparin sodium working method of the present invention.

Fig. 2 is the vertical view of Fig. 1.

Fig. 3 is the structural representation of independent archenteron-scrapping machine part in Fig. 1.

Fig. 4 is the vertical view of Fig. 3.

Fig. 5 is the structural representation that the heparin sodium adopted in enzymolysis operation in heparin sodium working method of the present invention produces enzymolysis device.

Fig. 6 is the A direction view that the heparin sodium adopted in enzymolysis operation in heparin sodium working method of the present invention produces the stir shaft installation supporting structure of enzymolysis device.

Fig. 7 is the partial enlarged drawing in Fig. 5.

Fig. 8 is that the heparin sodium adopted in elution procedure in heparin sodium working method of the present invention produces washing device structural representation.

Embodiment

Below in conjunction with accompanying drawing and concrete embodiment, the present invention is described in further detail.

As shown in Figures 1 to 8, a kind of embodiment of heparin sodium working method, comprise the large process of postcibal diarrhea, enzymolysis, absorption, wash-out and precipitation five, wherein namely enzymolysis operation have employed enzymolysis processing method of the present invention, in described postcibal diarrhea operation, the mucus scraped in casing is collected when carrying out striking to hair intestines and making its intestines skin and casing be broken, again poured water in casing inside after intestines skin is separated simultaneously and rinse and collect washing fluid, using described mucus and washing fluid all as the intestinal mucosa liquid in described enzymolysis operation;

In described elution procedure, adopt salt solution to carry out three grades of wash-outs to the resin leached from described absorption process, wherein third stage wash-out adopt new preparation salt solution (becoming elutriant after wash-out) and from the resin leached after the wash-out of the second stage, second stage wash-out adopts from the resin leached after the elutriant after third stage wash-out and first step wash-out, first step wash-out adopts from the elutriant after the wash-out of the second stage with from the resin leached in described absorption process, and the elutriant after first step wash-out enters follow-up described precipitation operation.

Wherein, when carrying out three grades of wash-outs in described elution procedure, third stage elution time is 1 hour, and second stage elution time is 1.5 hours, and first step elution time is 3 hours.

Wherein, in described enzymolysis operation, enzymatic vessel is adopted to carry out enzymolysis; First, the intestinal mucosa liquid feed valve on enzymatic vessel is opened; Then, the fresh intestinal mucosa liquid obtained in postcibal diarrhea operation is sent in enzymatic vessel, and intestinal mucosa liquid in enzymatic vessel is stirred; , intestinal mucosa liquid in enzymatic vessel is heated meanwhile, in intestinal mucosa liquid, progressively add alkali and the mixed liquid of synthesis converted by salt simultaneously, when heating to 47 ~ 53 DEG C, adding Sumizyme MP by mixed liquid enzymolysis processing is enzymolysis solution, controlled enzymatic hydrolysis liquid PH=8 ~ 9, and salinity is 3 ~ 3.5 degree; Be incubated 2 ~ 3 hours, be then warming up to 80 ± 5 DEG C, and insulation is no less than 30 minutes; Finally, stop stirring enzymolysis solution in enzymatic vessel, open enzymatic vessel baiting valve and enzymolysis solution is put into hay tank filter;

In described absorption process, first, described enzymolysis solution is sucked adsorption tanks, described enzymolysis solution is stirred, when described enzymolysis solution is cooled to 54 DEG C ~ 59 DEG C, add heparin adsorption resin, insulated and stirred absorption 8 ~ 10 hours;

In described elution procedure, polymeric adsorbent described in described absorption process is rinsed well, put into wash-out tank after being filtered dry and carry out wash-out, then add the salt solution identical with weight resin and convert synthesis batch mixing, described batch mixing is heated to 55 ~ 60 DEG C, carries out wash-out;

Wherein, in described postcibal diarrhea operation, first obtain heparin sodium processing postcibal diarrhea device (as shown in Figure 1 to Figure 4) of following structure, the archenteron-scrapping machine that described heparin sodium processing postcibal diarrhea device comprises frame 1 and is arranged in frame 1, described archenteron-scrapping machine comprises the left and right supporting base 2 being fixed on the left and right sides in frame 1, with the striking mechanism be horizontally installed on left and right supporting base 2 and haulage gear, described striking mechanism comprises the doctor roll 3 and auxiliary rubber roller 4 that are set up in parallel, haulage gear in described archenteron-scrapping machine, comprise a pair take off roll 5 be set up in parallel, take off roll 5 length direction is consistent with doctor roll 3 length direction and be positioned at the position, front lower place in doctor roll 3 and auxiliary rubber roller 4 gap, the termination of a pair take off roll 5 is provided with and engages each other and a pair gear 6 for driving two take off roll 5 relatively to rotate,

In described archenteron-scrapping machine, also comprise position-limit mechanism, described position-limit mechanism comprise transverse strands to be located on left and right supporting base 2 and between haulage gear and striking mechanism the support bar 7 of position, on support bar 7, forward upward is set side by side with the limited post 8 of several uniform intervals distribution;

In described archenteron-scrapping machine, the doctor roll be also provided with for being covered under it by doctor roll 3 renovates 19, and the two ends that described doctor roll renovates 19 front side edge are hinged on left and right supporting base 2 rotationally;

Described doctor roll renovates the viewing window 9 just doctor roll 3 top position being also provided with to glass material on 19;

Described heparin sodium processing postcibal diarrhea device also comprises the worktable of long strip shape, described archenteron-scrapping machine is positioned at worktable middle part, the table top of described worktable rear end section start is provided with primary launder 10, primary launder 10 Inner Front End position is each side provided with a water faucet 18 for previous cleaning, and in primary launder 10, rear end is provided with sewage outlet 11; Primary launder 10 is provided with receiving tank 12 with being also connected between archenteron-scrapping machine, and receiving tank 12 rear end has one for collecting the mucous membrane pond 13 of scraping intestinal mucosa; Worktable is positioned at archenteron-scrapping machine anterior position and is also provided with water flowing cleaning leak detection groove 14, described water flowing cleaning leak detection groove 14 Inner Front End position is each side provided with a water faucet 20 for water flowing leak detection, and in water flowing cleaning leak detection groove 14, rear end is also provided with leak detection liquid collecting tank 15;

Be positioned at water faucet rear position in described primary launder 10 and water flowing cleaning leak detection groove 14 to be provided with one and to lose small opening 16, lose activity in small opening 16 and be linked with one and lose leaky bucket 17;

Then operate according to following operation steps:

First, first the foreign material such as the loose fat of hair intestines surface attachment are extractd in primary launder 10, and the foreign material of excision are directly discarded in leaky bucket 17 by losing small opening 16; To be poured water flushing for the water faucet 18 of previous cleaning to connecting hair intestines one end, the waste water of flushing is by the outer row of sewage outlet 11 again; After rinsing, hair intestines deliver to receiving tank 12 forward, hair intestines are put along worktable length direction by receiving tank 12 and after being many along worktable width laid out in parallel on machine postcibal diarrhea;

Subsequently, when postcibal diarrhea, many hair intestines go up archenteron-scrapping machine side by side, and adjacent hair intestines spacing 15-25cm also leans on the limited post 8 of position-limit mechanism to separate; Start archenteron-scrapping machine, hair intestines termination is sent between the doctor roll 3 of striking mechanism and auxiliary rubber roller 4 and pass, forward downward is sent between a pair take off roll 5 of haulage gear again, a pair take off roll 5 are clamped mao intestines and are relatively rotated and provide tractive force automatically to pull mao intestines to carry out striking in striking mechanism, scrape the mucous membrane liquid made and flow to mucous membrane pond 13 by receiving tank 12 and regather;

Finally, hair intestines after striking deliver to forward water flowing cleaning leak detection groove 14, by manually the intestines skin of casing surface attachment being removed and putting into the basket (not shown in FIG.) of specifying, again remaining casing is adopted water faucet 20 water flowing cleaning, find during cleaning that the casing of leak or fragmentation need cut off, the water liquid cleaned out regathers after being pooled to leak detection liquid collecting tank 15.

Wherein, in described enzymolysis operation, the heparin sodium first obtaining following structure produces enzymolysis device (as shown in Figures 5 to 7), described heparin sodium is produced enzymolysis device and is comprised enzymatic vessel 1 ', the tank body top of described enzymatic vessel 1 ' is provided with manhole 2 ', lower end is provided with discharge port 3 ', enzymatic vessel 1 ' is also provided with whipping appts and heating unit, described tank body is also provided with the intestinal mucosa liquid liquid-inlet pipe 4 ', alkali lye liquid-inlet pipe 5 ', salt solution water inlet pipe 6 ' and the tap water water inlet pipe 7 ' that are communicated with tank inner chamber, described heparin sodium is produced enzymolysis device and is also comprised automatic detection and control system, described automatic detection and control system comprises the temperature sensor 11a ' for detecting temperature of charge be arranged in inner tank wall, for detecting the pH value detecting sensor 11b ' of material potential of hydrogen and the salometer 11c ' for detecting material salinity, described temperature sensor 11a ', pH value detecting sensor 11b ' is connected with salometer 11c ' all with control center 11d ' and provides detection data for it, described automatic detection and control system also comprises and is separately positioned on described intestinal mucosa liquid liquid-inlet pipe 4 ', alkali lye liquid-inlet pipe 5 ', salt solution water inlet pipe 6 ' and the electric-controlled switch valve 11e ' on tap water water inlet pipe 7 ', described intestinal mucosa liquid liquid-inlet pipe 4 ', alkali lye liquid-inlet pipe 5 ', salt solution water inlet pipe 6 ' separately with the electric-controlled switch valve 11e ' on tap water water inlet pipe 7 ' is all connected with described control center 11d ' and controls by it,

Described heating unit comprises the heating coil 8 ' arranged along the coiling of enzymatic vessel 1 ' inner tank wall, heating coil 8 ' has the inlet pipe 9a ' extending tank body from upper end, there is the escape pipe 9b ' extending tank body from lower end, described inlet pipe 9a ' is above provided with electrical control steam valve 10 ', and described electrical control steam valve 10 ' is connected with described control center 11d ' and controls by it;

Described whipping appts comprises the agitator motor 12 ' being positioned at tank body top, vertically be arranged on the stir shaft 13 ' in the middle part of tank inner chamber, by stir shaft, supporting structure 14 ' is installed between described agitator motor 12 ' and described stir shaft 13 ' to be connected, described stir shaft installs supporting structure 14 ', comprise and be upwards fixed on tank body top and overall tubular supporting base 14a ', supporting base 14a ' middle part has a horizontally disposed brace table 14b ', described agitator motor 12 ' is fixed on supporting base 14a ' top and electric machine main shaft extends down in supporting base 14a ', the brace table 14b ' at described supporting base 14a ' middle part is above rotatably mounted with a tunnel shaft 14c ' by bearing, end, tunnel shaft 14c ' upper end is docked and transmitting torque by concave-convex fit structure 14d ' with agitator motor 12 ' output shaft lower end, described tunnel shaft 14c ' lower end adjacent end position permanent sleeve is provided with on a circle and connects steel bushing 14e ', described stir shaft 13 ' upper end extends into the lower connection steel bushing 14f ' that the interior and permanent sleeve of supporting base 14a ' is provided with and upper connection steel bushing 14e ' correspondence, several cylinder lever connecting rod 14h ' of annular uniform setting between described upper connection steel bushing 14e ' and lower connection steel bushing 14f ', cylinder lever connecting rod 14h ' two ends adopt nut to be connected through described upper connection steel bushing 14e ' with lower connection steel bushing 14f ', between upper connection steel bushing 14e ' and lower connection steel bushing 14f ', also annular is evenly equipped with several whisker 14g ' simultaneously, the two ends up and down of described whisker 14g ' are fixed on connection steel bushing 14e ' and lower connection steel bushing 14f ' respectively,

The upper annular of described upper connection steel bushing 14e ' is furnished with several joint bolt 14i ', described joint bolt 14i ' front end edge connects steel bushing 14e ' outer side level and penetrates the connection interior realization of tunnel shaft 14c ' connecting steel bushing 14e ' and tunnel shaft 14c ';

On described tank body, manhole 2 ' place is also provided with manhole cover structure, described manhole cover structure to comprise along manhole 2 ' to tank body the outside circle eck 16 ' that convexes to form and surround cover type and be arranged on lid 17 ' on eck of stretching, lid 17 ' upper surface is diametrically fixedly installed a mounting rod 18 ', one end and the free bearing be fixed on eck 16 ' of mounting rod 18 ' are rotatably connected, the other end of mounting rod 18 ' extends lid 17 ' and is provided with the U-lag 19 ' that a horizontal section takes the shape of the letter U, the eck of U-lag 19 ' below correspondence is provided with a locking lever 20 ' rotationally, locking lever 20 ' outer end is also connected with a handles 21 ' by screw thread activity, locking lever 20 ' outer end upwards can rotate that to enter described U-lag 19 ' interior and be locked at U-lag 19 ' upper surface by rotating handles 21 ',

Then operate according to following operation steps:

First, the electric-controlled switch valve 11e ' charging of opening intestinal mucosa liquid liquid-inlet pipe 4 ' by control center 11d ', pours into intestinal mucosa liquid in enzymatic vessel 1 ';

Secondly, start the agitator motor 12 ' at tank body top, stir shaft 13 ' starts to stir; Meanwhile, the data of control center 11d ' automatic detection for temperature sensor 11a ', pH value detecting sensor 11b ' and salometer 11c ', and control the electric-controlled switch valve 11e ' on electrical control steam valve 10 ', alkali lye liquid-inlet pipe 5 ' and the electric-controlled switch valve 11e ' on salt solution water inlet pipe 6 ' respectively, such that the temperature of enzymolysis solution in tank maintains 47 ~ 53 DEG C, pH value maintains PH=8 ~ 9, salt angle value maintains 3 ~ 3.5 degree;

Finally, axle 13 ' to be mixed stirred after 2.5 hours, and control center 11d ' control electrical control steam valve 10 will be warming up to 80 DEG C in tank, and maintenance is no less than 30 minutes; Subsequently, close the agitator motor 12 ' at tank body top, stir shaft 13 ' stops stirring; Open enzymatic vessel 1 ' lower end and be provided with discharge port 3 ', enzymolysis solution is delivered to hay tank (not shown in FIG.).

Wherein, in described elution procedure, the heparin sodium first obtaining following structure produces washing device (as shown in Figure 8), described heparin sodium is produced washing device and is comprised wash-out tank 1 "; each wash-out tank 1 " lower end is provided with liquid discharge pipe 2 "; described liquid discharge pipe 2 " in cross-sectional direction is provided with filter screen 3 "; described liquid discharge pipe 2 " on lower than described filter screen 3 " is provided with the switch-valve 4 for controlling discharge opeing ", higher than described filter screen 3 " being also provided with bypass discharge nozzle 5 ", described bypass discharge nozzle 5 " on be provided with switch-valve 6 " for controlling discharging; Described wash-out tank 1 " is at least two and is concatenated into multilevel hierarchy; In described multilevel hierarchy, the described liquid discharge pipe 2 " by Drainage pipe 7 " of next stage wash-out tank is connected with the upper end of upper level wash-out tank, described Drainage pipe 7 " on be provided with electric pump 8 " for discharge opeing; The described bypass discharge nozzle 5 " by discharge pipe 9 " of upper level wash-out tank is connected with the upper end of next stage wash-out tank, described discharge pipe 9 " on be provided with electric pump 10 " for discharging;

Described multilevel hierarchy is tertiary structure, and described tertiary structure comprises first step wash-out tank 11 ", second stage wash-out tank 12 " and third stage wash-out tank 13 "; Described third stage wash-out tank 13 " described liquid discharge pipe 2 " is connected by the upper end of Drainage pipe 7 " with described second stage wash-out tank 12 ", and described second stage wash-out tank 12 " described liquid discharge pipe 2 " is connected by the upper end of Drainage pipe 7 " with described first step wash-out tank 11 "; Described first step wash-out tank 11 " described bypass discharge nozzle 5 " is connected by the upper end of discharge pipe 9 " with described second stage wash-out tank 12 ", and described second stage wash-out tank 12 " described bypass discharge nozzle 5 " is connected by the upper end of discharge pipe 9 " with described third stage wash-out tank 13 ";

Described wash-out tank 1 " on be also provided with stirring mechanism;

Described stirring mechanism comprises agitator motor 14 " and agitator arm 15 ", described agitator motor 14 " being arranged on wash-out tank 1 " upper end, and in described agitator arm 15 " stretching at wash-out tank 1 ", described agitator motor 14 " with described agitator arm 15 " connects;

Frame 16 is also comprised in described multilevel hierarchy ", on described wash-out tank 1 " being arranged on described frame 16 ", described frame 16 " on be also provided with helper 17 ";

Described wash-out tank 1 " upper end is provided with manhole 18 ";

Described wash-out tank 1 " is also provided with heating arrangements (not drawing in figure);

Then operate according to following operation steps:

Step one: open first step wash-out tank 11 " and third stage wash-out tank 13 " simultaneously and carry out wash-out.Wherein, first step wash-out tank 11 " the resin that leaches from described absorption process of resin, elutriant " filters the elutriant obtained from second stage wash-out tank 12 in last time circulation after wash-out; Filter the resin obtained after the wash-out of third stage wash-out tank 13 " resin from second stage wash-out tank 12 in last time circulation ", elutriant is from the fresh salt solution added; During wash-out, first step wash-out tank 11 " wash-out three hours, third stage wash-out tank 13 " wash-out 1 hour (in this process second stage wash-out tank 12 " is vacant).

Step 2: by first step wash-out tank 11 in step one " strip the elutriant leached and drain into the process of described precipitation operation, the resin leached drains into second stage wash-out tank 12 "; By third stage wash-out tank in step one 13 " strip leach elutriant drain into second stage wash-out tank 12 ", the resin leached collects recycling after arranging outward;

Step 3: open second stage wash-out tank 12 " carries out wash-out; elution time 1.5 hours (during wash-out, first step wash-out tank 11 " and third stage wash-out tank 13 " is vacant); after wash-out, the elutriant filtered out is drained into first step wash-out tank 11 ", and resin drains into third stage wash-out tank 13 ", circulate since then;

In above-mentioned three steps, described wash-out tank, when wash-out, is opened agitator motor 14 and " is stirred in tank, while stirring, open heating arrangements (not drawing in figure), mixed solution in tank body is heated to 55 ~ 60 DEG C.

Through test, carry out heparin sodium processing according to above-mentioned embodiment to extract, the heparin sodium first product that every 1500 chitterlings just can prepare one hundred million unit (referring to actives prime number) can be reached, therefore compared to the prior art, drastically increase heparin sodium extraction efficiency.

Claims

1. an enzymolysis processing method, is characterized in that, adopts enzymatic vessel to carry out enzymolysis; First, the intestinal mucosa liquid feed valve on enzymatic vessel is opened; Then, the fresh intestinal mucosa liquid obtained in postcibal diarrhea operation is sent in enzymatic vessel, and intestinal mucosa liquid in enzymatic vessel is stirred; , intestinal mucosa liquid in enzymatic vessel is heated meanwhile, in intestinal mucosa liquid, progressively add alkali and the mixed liquid of synthesis converted by salt simultaneously, when heating to 47 ~ 53 DEG C, adding Sumizyme MP by mixed liquid enzymolysis processing is enzymolysis solution, controlled enzymatic hydrolysis liquid pH=8 ~ 9, and salinity is 3 ~ 3.5 degree; Be incubated 2 ~ 3 hours, be then warming up to 80 ± 5 DEG C, and insulation is no less than 30 minutes; Finally, stop stirring enzymolysis solution in enzymatic vessel, open enzymatic vessel baiting valve and enzymolysis solution is put into hay tank filter;

Present method is specially: the heparin sodium first obtaining following structure produces enzymolysis device, described heparin sodium is produced enzymolysis device and is comprised enzymatic vessel (1 '), the tank body top of described enzymatic vessel (1 ') is provided with manhole (2 '), lower end is provided with discharge port (3 '), enzymatic vessel (1 ') is also provided with whipping appts and heating unit, it is characterized in that, described tank body is also provided with intestinal mucosa liquid liquid-inlet pipe (4 '), alkali lye liquid-inlet pipe (5 '), salt solution water inlet pipe (6 ') and the tap water water inlet pipe (7 ') that are communicated with tank inner chamber, described heparin sodium is produced enzymolysis device and is also comprised automatic detection and control system, described automatic detection and control system comprises the temperature sensor (11a ') for detecting temperature of charge be arranged in inner tank wall, for detecting the pH value detecting sensor (11b ') of material potential of hydrogen and the salometer (11c ') for detecting material salinity, described temperature sensor (11a '), pH value detecting sensor (11b ') is connected with salometer (11c ') all with control center (11d ') and provides detection data for it, described automatic detection and control system also comprises and is separately positioned on described intestinal mucosa liquid liquid-inlet pipe (4 '), alkali lye liquid-inlet pipe (5 '), electric-controlled switch valve (11e ') on salt solution water inlet pipe (6 ') and tap water water inlet pipe (7 '), described intestinal mucosa liquid liquid-inlet pipe (4 '), alkali lye liquid-inlet pipe (5 '), salt solution water inlet pipe (6 ') separately with the electric-controlled switch valve (11e ') on tap water water inlet pipe (7 ') is all connected with described control center (11d ') and controls by it,

Described heating unit comprises the heating coil (8 ') arranged along the coiling of enzymatic vessel (1 ') inner tank wall, heating coil (8 ') has the inlet pipe (9a ') of extending tank body from upper end, there is the escape pipe (9b ') extending tank body from lower end, described inlet pipe (9a ') is provided with electrical control steam valve (10 '), and described electrical control steam valve (10 ') is connected with described control center (11d ') and controls by it;

Described whipping appts comprises the agitator motor (12 ') being positioned at tank body top, vertically be arranged on the stir shaft (13 ') in the middle part of tank inner chamber, by stir shaft, supporting structure (14 ') is installed between described agitator motor (12 ') and described stir shaft (13 ') to be connected, described stir shaft installs supporting structure (14 '), comprise and be upwards fixed on tank body top and overall tubular supporting base (14a '), supporting base (14a ') middle part has a horizontally disposed brace table (14b '), described agitator motor (12 ') is fixed on supporting base (14a ') top and electric machine main shaft extends down in supporting base (14a '), the brace table (14b ') at described supporting base (14a ') middle part is upper is rotatably mounted with a tunnel shaft (14c ') by bearing, tunnel shaft (14c ') end, upper end is docked and transmitting torque by concave-convex fit structure (14d ') with agitator motor (12 ') output shaft lower end, described tunnel shaft (14c ') lower end adjacent end position permanent sleeve is provided with on a circle and connects steel bushing (14e '), described stir shaft (13 ') upper end extends into the lower connection steel bushing (14f ') that the interior also permanent sleeve of supporting base (14a ') is provided with and upper connection steel bushing (14e ') correspondence, several cylinder lever connecting rods (14h ') of annular uniform setting between described upper connection steel bushing (14e ') and lower connection steel bushing (14f '), cylinder lever connecting rod (14h ') two ends adopt nut to be connected through described upper connection steel bushing (14e ') with lower connection steel bushing (14f '), between upper connection steel bushing (14e ') and lower connection steel bushing (14f '), also annular is evenly equipped with several whisker (14g ') simultaneously, the two ends up and down of described whisker (14g ') are fixed on connection steel bushing (14e ') and lower connection steel bushing (14f ') respectively,

The upper annular of described upper connection steel bushing (14e ') is furnished with several joint bolt (14i '), described joint bolt (14i ') front end edge connects steel bushing (14e ') outer side level and penetrates in tunnel shaft (14c ') connection realizing connecting steel bushing (14e ') and tunnel shaft (14c ');

On described tank body, manhole (2 ') place is also provided with manhole cover structure, described manhole cover structure to comprise along manhole (2 ') to tank body outside stretch circle eck (16 ') that convexes to form and encirclement cover type and is arranged on lid (17 ') on eck, lid (17 ') upper surface is diametrically fixedly installed a mounting rod (18 '), one end and the free bearing be fixed on eck (16 ') of mounting rod (18 ') are rotatably connected, the other end of mounting rod (18 ') extends lid (17 ') and is provided with the U-lag (19 ') that a horizontal section takes the shape of the letter U, the eck that U-lag (19 ') below is corresponding is provided with a locking lever (20 ') rotationally, locking lever (20 ') outer end is also connected with a handles (21 ') by screw thread activity, locking lever (20 ') outer end upwards can be rotated and entered in described U-lag (19 ') and be locked at U-lag (19 ') upper surface by rotating handles (21 '),

Then operate according to following operation steps:

First, electric-controlled switch valve (11e ') charging of opening intestinal mucosa liquid liquid-inlet pipe (4 ') by control center (11d '), pours into intestinal mucosa liquid in enzymatic vessel (1 ');

Secondly, start the agitator motor (12 ') at tank body top, stir shaft (13 ') starts to stir; Meanwhile, the data of control center (11d ') automatic detection for temperature sensor (11a '), pH value detecting sensor (11b ') and salometer (11c '), and control electrical control steam valve (10 '), the electric-controlled switch valve (11e ') on alkali lye liquid-inlet pipe (5 ') and the electric-controlled switch valve (11e ') on salt solution water inlet pipe (6 ') respectively, such that the temperature of enzymolysis solution in tank maintains 47 ~ 53 DEG C, pH value maintains pH=8 ~ 9, salt angle value maintains 3 ~ 3.5 degree;

Finally, axle (13 ') to be mixed is stirred after 2.5 hours, and control center (11d ') controls electrical control steam valve (10) and will be warming up to 80 DEG C in tank, and maintenance is no less than 30 minutes; Subsequently, close the agitator motor (12 ') at tank body top, stir shaft (13 ') stops stirring; Open enzymatic vessel (1 ') lower end and be provided with discharge port (3 '), enzymolysis solution is delivered to hay tank.

2. a heparin sodium working method, comprise the large process of postcibal diarrhea, enzymolysis, absorption, wash-out and precipitation five, it is characterized in that, in described postcibal diarrhea operation, the mucus scraped in casing is collected when carrying out striking to hair intestines and making its intestines skin and casing be broken, again poured water in casing inside after intestines skin is separated simultaneously and rinse and collect washing fluid, using described mucus and washing fluid all as the intestinal mucosa liquid in described enzymolysis operation;

In described elution procedure, adopt salt solution to carry out three grades of wash-outs to the resin leached from described absorption process, wherein third stage wash-out adopt new preparation salt solution and from the resin leached after the wash-out of the second stage, second stage wash-out adopts from the resin leached after the elutriant after third stage wash-out and first step wash-out, first step wash-out adopts from the elutriant after the wash-out of the second stage with from the resin leached in described absorption process, and the elutriant after first step wash-out enters follow-up described precipitation operation; When carrying out three grades of wash-outs in described elution procedure, third stage elution time is 1 hour, and second stage elution time is 1.5 hours, and first step elution time is 3 hours; Described enzymolysis operation adopts enzymolysis processing method according to claim 1 to carry out.

3. heparin sodium working method as claimed in claim 2, is characterized in that, in described absorption process, first, described enzymolysis solution is sucked adsorption tanks, described enzymolysis solution is stirred, when described enzymolysis solution is cooled to 54 DEG C ~ 59 DEG C, add heparin adsorption resin, insulated and stirred absorption 8 ~ 10 hours;

4. heparin sodium working method as claimed in claim 3, it is characterized in that, in described postcibal diarrhea operation, first obtain the heparin sodium processing postcibal diarrhea device of following structure, the archenteron-scrapping machine that described heparin sodium processing postcibal diarrhea device comprises frame (1) and is arranged in frame (1), described archenteron-scrapping machine comprises the left and right supporting base (2) being fixed on the left and right sides in frame 1, with the striking mechanism be horizontally installed on left and right supporting base (2) and haulage gear, described striking mechanism comprises the doctor roll (3) and auxiliary rubber roller (4) that are set up in parallel, it is characterized in that, haulage gear in described archenteron-scrapping machine, comprise a pair take off roll (5) be set up in parallel, take off roll (5) length direction is consistent with doctor roll (3) length direction and be positioned at the position, front lower place in doctor roll (3) and auxiliary rubber roller (4) gap, the termination of a pair take off roll (5) is provided with and engages each other and a pair gear (6) for driving two take off roll (5) relatively to rotate,

In described archenteron-scrapping machine, also comprise position-limit mechanism, described position-limit mechanism comprises transverse strands and is located at the upper and support bar (7) that is position between haulage gear and striking mechanism of left and right supporting base (2), and the upper forward upward of support bar (7) is set side by side with the limited post (8) of several uniform intervals distribution;

Also be provided with for being renovated (19) by doctor roll (3) doctor roll be covered under it in described archenteron-scrapping machine, the two ends that described doctor roll renovates (19) front side edge are hinged on left and right supporting base (2) rotationally;

Described doctor roll renovates the viewing window (9) (19) being just also provided with glass material to doctor roll (3) top position;

Described heparin sodium processing postcibal diarrhea device also comprises the worktable of long strip shape, described archenteron-scrapping machine is positioned at worktable middle part, the table top of described worktable rear end section start is provided with primary launder (10), primary launder (10) Inner Front End position is each side provided with a water faucet for previous cleaning (18), and primary launder (10) interior rear end is provided with sewage outlet (11); Primary launder (10) is provided with receiving tank (12) with being also connected between archenteron-scrapping machine, and receiving tank (12) rear end has one for collecting the mucous membrane pond (13) of scraping intestinal mucosa; Worktable is positioned at archenteron-scrapping machine anterior position and is also provided with water flowing cleaning leak detection groove (14), described water flowing cleaning leak detection groove (14) Inner Front End position is each side provided with a water faucet (20) for water flowing leak detection, and water flowing cleaning leak detection groove (14) interior rear end is also provided with leak detection liquid collecting tank (15);

Be positioned at water faucet rear position in described primary launder (10) and water flowing cleaning leak detection groove (14) to be provided with one and to lose small opening (16), lose small opening (16) interior activity and be linked with one and lose leaky bucket (17);

Then operate according to following operation steps:

First, first the foreign material such as the loose fat of hair intestines surface attachment are extractd in primary launder (10), and the foreign material of excision are directly discarded in leaky bucket (17) by losing small opening (16); To be poured water flushing for the water faucet (18) of previous cleaning to connecting hair intestines one end, the waste water of flushing is arranged outward by sewage outlet (11) again; After rinsing, hair intestines deliver to receiving tank (12) forward, hair intestines are put along worktable length direction by receiving tank (12) and goes up machine postcibal diarrhea after being many along worktable width laid out in parallel;

Subsequently, when postcibal diarrhea, many hair intestines go up archenteron-scrapping machine side by side, and adjacent hair intestines spacing 15-25cm also leans on the limited post (8) of position-limit mechanism to separate; Start archenteron-scrapping machine, hair intestines termination is sent between the doctor roll (3) of striking mechanism and auxiliary rubber roller (4) and pass, forward downward is sent between a pair take off roll (5) of haulage gear again, a pair take off roll (5) is clamped mao intestines and is relatively rotated and provide tractive force automatically to pull mao intestines to carry out striking in striking mechanism, scrapes the mucous membrane liquid made and flow to mucous membrane pond (13) by receiving tank (12) and regather;

Finally, hair intestines after striking deliver to forward water flowing cleaning leak detection groove (14), by manually the intestines skin of casing surface attachment being removed and putting into the basket of specifying, again remaining casing is adopted water faucet (20) water flowing cleaning, find during cleaning that the casing of leak or fragmentation need cut off, the water liquid cleaned out regathers after being pooled to leak detection liquid collecting tank (15).

5. heparin sodium working method as claimed in claim 3, it is characterized in that, in described enzymolysis operation, the heparin sodium first obtaining following structure produces enzymolysis device, described heparin sodium is produced enzymolysis device and is comprised enzymatic vessel (1 '), the tank body top of described enzymatic vessel (1 ') is provided with manhole (2 '), lower end is provided with discharge port (3 '), enzymatic vessel (1 ') is also provided with whipping appts and heating unit, it is characterized in that, described tank body is also provided with intestinal mucosa liquid liquid-inlet pipe (4 ') be communicated with tank inner chamber, alkali lye liquid-inlet pipe (5 '), salt solution water inlet pipe (6 ') and tap water water inlet pipe (7 '), described heparin sodium is produced enzymolysis device and is also comprised automatic detection and control system, described automatic detection and control system comprises the temperature sensor (11a ') for detecting temperature of charge be arranged in inner tank wall, for detecting the pH value detecting sensor (11b ') of material potential of hydrogen and the salometer (11c ') for detecting material salinity, described temperature sensor (11a '), pH value detecting sensor (11b ') is connected with salometer (11c ') all with control center (11d ') and provides detection data for it, described automatic detection and control system also comprises and is separately positioned on described intestinal mucosa liquid liquid-inlet pipe (4 '), alkali lye liquid-inlet pipe (5 '), electric-controlled switch valve (11e ') on salt solution water inlet pipe (6 ') and tap water water inlet pipe (7 '), described intestinal mucosa liquid liquid-inlet pipe (4 '), alkali lye liquid-inlet pipe (5 '), salt solution water inlet pipe (6 ') separately with the electric-controlled switch valve (11e ') on tap water water inlet pipe (7 ') is all connected with described control center (11d ') and controls by it,

Then operate according to following operation steps:

6. heparin sodium working method as claimed in claim 3, it is characterized in that, in described elution procedure, the heparin sodium first obtaining following structure produces washing device, described heparin sodium is produced washing device and is comprised wash-out tank (1 "), each wash-out tank (1 ") lower end is provided with liquid discharge pipe (2 "), in described liquid discharge pipe (2 "), cross-sectional direction is provided with filter screen (3 "), described liquid discharge pipe (2 ") is provided with switch-valve (4 ") for controlling discharge opeing lower than described filter screen (3 "), bypass discharge nozzle (5 ") is also provided with higher than described filter screen (3 "), described bypass discharge nozzle (5 ") is provided with switch-valve for controlling discharging (6 "), (1 ") is at least two and is concatenated into multilevel hierarchy described wash-out tank, in described multilevel hierarchy, the described liquid discharge pipe (2 ") of next stage wash-out tank is connected with the upper end of upper level wash-out tank by Drainage pipe (7 "), described Drainage pipe (7 ") is provided with the electric pump (8 ") for discharge opeing, the described bypass discharge nozzle (5 ") of upper level wash-out tank is connected with the upper end of next stage wash-out tank by discharge pipe (9 "), described discharge pipe (9 ") is provided with the electric pump (10 ") for discharging,

Described multilevel hierarchy is tertiary structure, and described tertiary structure comprises first step wash-out tank (11 "), second stage wash-out tank (12 ") and third stage wash-out tank (13 "); The described liquid discharge pipe of described third stage wash-out tank (13 ") (2 ") is connected by the upper end of Drainage pipe (7 ") with described second stage wash-out tank (12 "), and the described liquid discharge pipe of described second stage wash-out tank (12 ") (2 ") is connected by the upper end of Drainage pipe (7 ") with described first step wash-out tank (11 "); The described bypass discharge nozzle of described first step wash-out tank (11 ") (5 ") is connected by the upper end of discharge pipe (9 ") with described second stage wash-out tank (12 "), and the described bypass discharge nozzle of described second stage wash-out tank (12 ") (5 ") is connected by the upper end of discharge pipe (9 ") with described third stage wash-out tank (13 ");

Described wash-out tank (1 ") is also provided with stirring mechanism;

Described stirring mechanism comprises agitator motor (14 ") and agitator arm (15 "), described agitator motor (14 ") is arranged on wash-out tank (1 ") upper end, described agitator arm (15 ") stretches in wash-out tank (1 "), and described agitator motor (14 ") connects with described agitator arm (15 ");

Also comprise in described multilevel hierarchy frame (16 "), described wash-out tank (1 ") is arranged in described frame (16 "), described frame (16 ") is also provided with helper (17 ");

Described wash-out tank (1 ") upper end is provided with manhole (18 ");

(1 ") is also provided with heating arrangements to described wash-out tank;

Then operate according to following operation steps:

When above-mentioned heparin sodium produces washing device use, circulation performs following steps:

Step one: simultaneously open first step wash-out tank (11 ") and third stage wash-out tank (13 ") carries out wash-out; Wherein, and first step wash-out tank (resin that the resin of 11 ") leaches from described absorption process, elutriant (filters the elutriant obtained after 12 ") wash-out from second stage wash-out tank in last time circulation; The resin of third stage wash-out tank (13 ") filters from after second stage wash-out tank in last time circulation (12 ") wash-out the resin obtained, and elutriant is from the fresh salt solution added; During wash-out, first step wash-out tank (11 ") wash-out three hours, third stage wash-out tank (13 ") wash-out 1 hour;

Step 2: (11 ") strip the elutriant leached and drain into the process of described precipitation operation, and the resin leached drains into second stage wash-out tank (12 ") by first step wash-out tank in step one; Third stage wash-out tank (13 ") in step one is stripped leach elutriant drain into second stage wash-out tank (12 "), the resin leached collects recycling after arranging outward;

Step 3: open second stage wash-out tank (12 ") carry out wash-out, elution time 1.5 hours, after wash-out, the elutriant filtered out is drained into first step wash-out tank (11 "), resin drains into third stage wash-out tank, and (13 "), circulate since then;

In above-mentioned three steps, described wash-out tank is when wash-out, and (14 ") are stirred in tank, while stirring, open heating arrangements, and mixed solution in tank body is heated to 55 ~ 60 DEG C to open agitator motor.