CN103598871B - Ophthalmic testing device - Google Patents
Ophthalmic testing device Download PDFInfo
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- CN103598871B CN103598871B CN201310611738.7A CN201310611738A CN103598871B CN 103598871 B CN103598871 B CN 103598871B CN 201310611738 A CN201310611738 A CN 201310611738A CN 103598871 B CN103598871 B CN 103598871B
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- raman
- slit lamp
- raman spectrometer
- lamp microscope
- examination apparatus
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Abstract
The invention discloses an ophthalmic testing device which is integrated with a Raman spectrometer and a slit-lamp microscope and can be used for ophthalmic basic test and clinical diagnosis and treatment. The ophthalmic testing device mainly comprises the slit-lamp microscope and the Raman spectrometer which are integrated by the principles of optics. The slit-lamp microscope and the Raman spectrometer can be assembled by a simple connecting device or a method of sharing an optical path and the like. Through effective utilization, modification and assembly of existing instruments, the slit-lamp microscope and the Raman spectrometer are integrated, the whole ophthalmic testing device is simple in structure and easy to operate, hardware support is provided for in-vivo application of Raman spectroscopy, and a novel testing device is intruded into the ophthalmology.
Description
Technical field
The invention belongs to instrument invention field is and in particular to a kind of eye examination apparatus based on Raman slit lamp microscope
Background technology
Slit lamp microscope is a kind of binocular microscope, can the three-dimensional structure clearly checking eye.Ophthalmologist's application is split
Gap lamp microscope can routinely or under emergency situations carry out the inspection of eye, guards normal eye care, diagnosis and the various eyes of discriminating
Disease, mixes the photograph that contact lenss contribute to ophthalmologic operation and eye.Slit lamp is to evaluation corneal injury, anterior chamber of eye pathology and foreign body
Significant.Some adnexaes such as applanation tonometer, imaging device, various prism are used for special inspection, increased
The range of slit lamp microscope.Slit lamp microscope can provide various types of illuminations, amplification, stereoscopic vision and select poly-
Burnt.Control the position of light beam and correct microscope, the privileged sites of observable eye.Slit lamp microscope is still provided that in ocular structure
Portion and outside image, the cross-sectional image of display hyaline tissue.The invention of slit lamp microscope makes ophthalmology obtain considerable sending out
Exhibition, the improving each time all in the development promoting whole ophthalmology of slit lamp microscope.
Raman spectrum, is a kind of scattering spectrum.Raman spectrum analyses method is based on Raman scattering effect, to incident optical frequency
The different scattering spectrum of rate is analyzed to obtain molecular vibration, rotation aspect information, and is applied to the one of molecular structure research
Plant analysis method.Raman spectroscopy as a kind of new qualitative, quantitative optical technology, because its Noninvasive, detection are required
The advantage such as sample volume is little is for the favor of field of ophthalmology researcher.At present, scientist used Raman spectrum cornea, tear,
The aspects such as sclera, aqueous humor, iris, crystalline lenses, vitreous body, retina carried out multiple researchs, included zoopery, people among these
Body sample experiment in vitro and body research.The technology and equipment related to Raman spectrum continuously emerges, and this has expanded Raman spectrum
Range of application.It must be the important of this technology following that Raman spectroscopy is more broadly applicable to ophthalmology basic and clinic studies
Developing direction.
Slit lamp microscope and Raman spectrometer are integrated by simple connecting device, the method is simple, not only expands
The scope of application of slit lamp microscope, introduces a kind of new auxiliary examination methods for ophthalmology simultaneously, and more ophthalmology introduces one
Plant the New Century Planned Textbook of understanding disease.This device can be not only used for scientific research experiment, can answer with improving of Raman spectroscopy again
For clinic diagnosis.
Content of the invention
The present invention is a kind of eye examination apparatus integrating Raman spectrometer and slit lamp microscope it is intended to provide for ophthalmology
A kind of method recognizing ocular physiology from molecular level.
Technical scheme is as follows：
A kind of eye examination apparatus, including a slit lamp microscope and a Raman spectrometer, wherein, slit lamp is micro-
Mirror is connected together by connecting integrating apparatus with Raman spectrometer, and described connection integrating apparatus can be burnt by slit lamp microscope
Point and Raman spectrometer focus overlap.
In the preferred embodiment, also include data handling system.
In the preferred embodiment, described slit lamp microscope is that HAAG-STREIT BQ900 slit lamp shows
Micro mirror, described Raman spectrometer is BWS415i-Raman Portable Raman spectrometer.
In the preferred embodiment, the upper end of slit lamp microscope main shaft is provided with a pallet, and pallet is provided with and turns
Platform, passes through screw pivot joint between one end of turntable, pallet and the upper-center of main shaft；The other end of turntable is fixed with background light source
Bar；
In the preferred embodiment, the first half of this background light source bar is provided with hole axle structure and screw, its work
With for adjusting Raman probe height.Top is provided with a transverse tube, and this transverse tube is hollow, can accommodate the probe portion of Raman spectrometer
Probe enter, in addition, being radially respectively equipped with two screw holes, screw hole above is provided with set screw, visits for fixing
Head；The upper end of following screw hole and background light source bar connects.
In the preferred embodiment, the Raman spectrometer integrated in this device and slit lamp microscope can bases
Need to select and increase different optics or non-optical auxiliary equipment, such as camera lens.
The using method of aforesaid eye examination apparatus, comprises the steps：
（1）Install connection integrating apparatus, slit lamp microscope focus and Raman spectrometer focus are overlapped；
（2）Start Raman spectrometer, probe portion switchs temporary close；
（3）Open slit lamp microscope, slit lamp light focus are focused on eye position to be detected or composition, closing is split
Gap lamp source, opens Raman spectrometer probe portion switch, now the hot spot of Raman spectrometer focuses on position to be detected or composition
On；
（4）Start Raman spectroscopy scans, form the Raman spectrum waveform of this position or composition.
In the preferred embodiment, this Raman slit lamp microscope directly can carry out routine to ocular tissue with composition
Raman spectrum detects, is possible with nanoparticle and carries out strengthening Raman spectrum detection.
In the preferred embodiment, the nanoparticle used in surface enhanced raman spectroscopy detection can be received for silver
Rice corpuscles, golden nanometer particle or other species synthesizing nano-particles.
In the preferred embodiment, the nanoparticle used in surface enhanced raman spectroscopy detection can directly drip
Plus or spray into ocular, or mixed after tear is suctioned out after detected.
In the preferred embodiment, step（5）Afterwards, also include step（6）, using this Raman slit lamp ophthalmology
The detection to normal or abnormal part tissue of eye and composition for the check device, sets up corresponding ocular tissue's Raman spectrum data storehouse.
In the present invention, the design of device is overlapping as mainly former by slit lamp microscope focus and Raman spectrometer focus
Then, realize the Raman spectrum imaging of position to be detected, composition to eye, the Raman spectrometer integrated in this device can be according to inspection
Survey the exciting light needing using different wave length, different detection power and detection time of exposure, simultaneously also dependent on needing to select
Select other optics such as different camera lenses or non-optical auxiliary equipment.By this Raman slit lamp microscope can directly to ocular tissue with
Composition carries out normal Raman spectral detection, is possible with nanoparticle and carries out strengthening Raman spectrum detection, the nanoparticle being used
Son can be Nano silver grain, golden nanometer particle or other species synthesizing nano-particles.The nanoparticle being used can direct Deca
Or spray into ocular, or mixed after tear is suctioned out after detected.
The present invention has not only expanded the scope of application of slit lamp microscope, introduces a kind of new auxiliary examination for ophthalmology simultaneously
Means, using this detection to normal or abnormal part tissue of eye and composition for Raman slit lamp eye examination apparatus, can set up corresponding
Ocular tissue's Raman spectrum data storehouse.The present invention can provide a kind of method recognizing ocular physiology from molecular level for ophthalmology.
Brief description
Fig. 1 is the structural representation of the major part of device in embodiment；
Fig. 2 is the top view of Fig. 1；
Fig. 3 is the perspective view of background light source bar in embodiment；
Fig. 4 is the cross-sectional view of background light source bar in embodiment；
Fig. 5 is healthy tear component Nano silver grain surface enhanced raman spectroscopy figure
In figure
1- slit lamp microscope eyepiece
2- slit lamp microscope object lens
3- Raman spectrometer probe portion
3-1 pops one's head in
4- slit lamp microscope background light source bar
5- slit lamp microscope light source
6- connecting rod top set screw
7- connecting rod middle and upper part screw
8- optics of slit-lamp axle
9- transverse tube
10- hole axle structure
11- main shaft
12- microscope axle
13- light source axle
14- pallet
15- turntable
Specific embodiment
Below by way of specific embodiment, technical scheme is further detailed and describes.
Referring to Fig. 1 to Fig. 4, with HAAG-STREIT BQ900 slit lamp microscope and the portable Raman of BWS415i-Raman
As a example the integration of spectrogrph and use.
HAAG-STREIT BQ900 slit lamp microscope includes main shaft 11, and main shaft 11 is provided with microscope axle 12, microscope
Axle 12 can rotate around main shaft 11, and upper end is provided with eyepiece 1 and object lens 2.Main shaft 11 is additionally provided with light source axle 13, and this light source axle 13 can
To rotate around main shaft 11, and can rotate around microscope axle 12 simultaneously.The upper end of this light source axle 13 is provided with light source 5.
The upper end of main shaft 11 is provided with a pallet 14, and pallet 14 is provided with turntable 15, one end of turntable 15, pallet and main shaft
Pass through screw 16 pivot joint between upper-center.The other end of turntable is fixed with background light source bar 4.
The first half of this background light source bar 4 is provided with hole axle structure 10 and screw 7, hole axle structure 10 and its work of screw 7
Adjusting Raman probe height with being, top is provided with a transverse tube 9, and this transverse tube 9 is hollow, can accommodate Raman spectrometer
The probe 3-1 of probe portion 3 enters, in addition, being radially respectively equipped with two screw holes, screw hole above is provided with set screw
6, for fixing probe 3-1, keep stable.The upper end of following screw hole and background light source bar 4 connects.
The assembling of the present invention is as follows：
1st, HAAG-STREIT BQ900 slit lamp microscope and BWS415i-Raman Portable Raman spectrometer pass through one
The connecting rod 4 that root is securable on HAAG-STREIT BQ900 slit lamp, transverse tube 9 carry the portable Raman of BWS415i-Raman
Spectrogrph probe portion 3 connects together.
2nd, connecting rod 4, transverse tube 9 form for hard material.Connecting rod height is set with slit lamp microscope optic axises distance
Put slit lamp microscope focus and Raman spectrometer focus can be overlapped as principle.For example, with background light source 5 as reference point,
Make connecting rod height, distance setting with extension light path be located at the focus of eyepiece 1 and light source 5 extension on as reference
Point.
3rd, connecting rod top ends set up transverse tube 9 separately, and the latter half structure is carried on the back with HAAG-STREIT BQ900 slit lamp microscope
Scape light source bar structure is identical.
4th, connecting rod top transverse tube 9 and the latter half are secured together by screw thread structure.Top transverse tube diameter with
Just allow the insertion of BWS415i-Raman Portable Raman spectrometer probe portion metallic rod, length is can achieve HAAG-STREIT
BQ900 slit lamp microscope and BWS415i-Raman Portable Raman spectrometer optical focus are principle.
5th, connecting rod the latter half can be directly inserted into HAAG-STREIT BQ900 slit lamp microscope and insert background light source
In the axis hole of bar, and can rotate around this axle, can dismantle at any time.
Inspection method and step
1st, with the background light source bar of connecting rod 4 replacement HAAG-STREIT BQ900 slit lamp microscope, by BWS415i-
The metallic rod of Raman Portable Raman spectrometer probe portion 3 is inserted in the transverse tube of top, tightens above transverse tube screw 6 with fixation.
2nd, BWS415i-Raman Portable Raman spectrometer and its data handling system are started, BWS415i-Raman is portable
Formula Raman spectrometer probe portion switchs temporary close.
3rd, open slit lamp microscope, slit lamp light focus are focused on eye position to be detected or composition, closing is split
Gap lamp source, opens Raman spectrometer probe portion switch, now the hot spot of Raman spectrometer focuses on position to be detected or composition
On.
4th, start Raman spectroscopy scans, form the Raman spectrum waveform of this position or composition.
Claims (10)
1. a kind of eye examination apparatus, including a slit lamp microscope and a Raman spectrometer, wherein, slit lamp microscope
Connected together by connecting integrating apparatus with Raman spectrometer, described connection integrating apparatus can be by slit lamp microscope focus
Overlap with Raman spectrometer focus；Open slit lamp microscope, by slit lamp light focus focus on eye position to be detected or
Composition, closes crack lamp source, opens Raman spectrometer probe portion switch, now the hot spot of Raman spectrometer focus on to be detected
On position or composition.
2. a kind of eye examination apparatus according to claim 1 are it is characterised in that may include or not including data processing system
System.
3. a kind of eye examination apparatus according to claim 1 are it is characterised in that the Raman spectrum integrated in this device
Instrument and slit lamp microscope can select and increase different optics or non-optical auxiliary equipment as needed.
4. the using method of the eye examination apparatus of claim 1, comprises the steps：
(1) install connection integrating apparatus, slit lamp microscope focus and Raman spectrometer focus are overlapped；
(2) start Raman spectrometer, probe portion switchs temporary close；
(3) open slit lamp microscope, slit lamp light focus are focused on eye position to be detected or composition, close slit lamp
Light source, opens Raman spectrometer probe portion switch, now the hot spot of Raman spectrometer focuses on position to be detected or composition；
(4) start Raman spectroscopy scans, form the Raman spectrum waveform of this position or composition.
5. a kind of using method of eye examination apparatus according to claim 4 is it is characterised in that integrated in this device
The Raman spectrometer exciting light that can need using different wave length according to detection.
6. a kind of using method of eye examination apparatus according to claim 4 is it is characterised in that integrated in this device
Raman spectrometer and slit lamp microscope every detection parameter can be adjusted according to detection needs, described detection parameter can
Including time of exposure and power.
7. a kind of using method of eye examination apparatus according to claim 4 is it is characterised in that this eye examination apparatus
Directly with composition, normal Raman spectral detection can be carried out to ocular tissue, be possible with nanoparticle and carry out strengthening Raman spectrum inspection
Survey.
8. a kind of using method of eye examination apparatus according to claim 7 is it is characterised in that strengthen Raman spectrum inspection
Surveying used nanoparticle is Nano silver grain, golden nanometer particle or other species synthesizing nano-particles.
9. a kind of using method of eye examination apparatus according to claim 7 is it is characterised in that strengthen Raman spectrum inspection
Survey used nanoparticle can direct Deca or spray into ocular, or mixed after tear is suctioned out after detected.
10. a kind of using method of eye examination apparatus according to claim 4 is it is characterised in that examined using this ophthalmology
Look into the detection to normal or abnormal part tissue of eye and composition for the device, corresponding ocular tissue's Raman spectrum data storehouse can be set up.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310611738.7A CN103598871B (en) | 2013-11-26 | 2013-11-26 | Ophthalmic testing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310611738.7A CN103598871B (en) | 2013-11-26 | 2013-11-26 | Ophthalmic testing device |
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| CN103598871A CN103598871A (en) | 2014-02-26 |
| CN103598871B true CN103598871B (en) | 2017-02-15 |
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| CN201310611738.7A Active CN103598871B (en) | 2013-11-26 | 2013-11-26 | Ophthalmic testing device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2015105457A1 (en) * | 2014-01-13 | 2015-07-16 | Nanyang Technological University | Device for determining a condition of an organ and method of operating the same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1668260A (en) * | 2002-07-18 | 2005-09-14 | 里尔科学技术大学 | Apparatus for treating age-related maculopathy (ARM) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20020095257A1 (en) * | 2000-03-27 | 2002-07-18 | Rosen Richard B. | Method and system for detection by raman measurements of bimolecular markers in the vitreous humor |
| JP4113035B2 (en) * | 2003-04-25 | 2008-07-02 | 株式会社ニデック | Medical laser equipment |
| US8403918B2 (en) * | 2010-04-01 | 2013-03-26 | John Taboada | Automated non-invasive capsulectomy and anterior segment surgical apparatus and method |
| EP2712299A4 (en) * | 2011-04-29 | 2014-12-10 | Gen Hospital Corp | Methods and arrangements for obtaining information and providing analysis for biological tissues |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1668260A (en) * | 2002-07-18 | 2005-09-14 | 里尔科学技术大学 | Apparatus for treating age-related maculopathy (ARM) |
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Effective date of registration: 20211229 Address after: 310000 room 406, building 19, No. 260, Baiyang street, Qiantang new area, Hangzhou, Zhejiang Patentee after: Hangzhou Purui Mingshi Technology Co.,Ltd. Address before: 361000 Siming South Road, Xiamen, Fujian Province, No. 422 Patentee before: XIAMEN University |