CN103592265A - Transmission type scanning method for biological chip - Google Patents
Transmission type scanning method for biological chip Download PDFInfo
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- CN103592265A CN103592265A CN201310577074.7A CN201310577074A CN103592265A CN 103592265 A CN103592265 A CN 103592265A CN 201310577074 A CN201310577074 A CN 201310577074A CN 103592265 A CN103592265 A CN 103592265A
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Abstract
The invention discloses a transmission type scanning method for a biological chip. The method comprises the following steps: (1) scanning is implemented by using a scanning instrument in a transmission type scanning mode; before scanning, an optical signal receiver mounted under the biological chip receives incident light; the optical signal strength IO is measured; (2) when scanning, after the incident light passes through a chip solid phase substrate and a chip array point, the optical signal receiver receives transmission light; the transmission light signal strength I is measured; after light ray passes through the chip solid phase substrate and the chip array point, the light strength is weakened; the weakening ratio is I/IO; (3) the content c of a solid phase matter and the corresponding weakening ratio I/IO are measured; a standard curve c-I/IO is drawn; (4) the content c' of the solid phase matter is determined through the comparison between one weakening ratio I/IO' and the standard curve c-I/IO; (5) the content of all components to be measured in a sample is calculated according to the obtained content c' of the solid phase matter and the sample dilution ratio.
Description
Technical field
The present invention relates to biomedical field of immunodetection, be specifically related to the detection method of the biochip of many indexs in a kind of visual detection testing sample.
Background technology
Existing biochip test method mainly contains fluorescence method, chemoluminescence method, catalysis development process etc.Fluorescence method is mainly carried out the detection of signal by laser co-focusing fluorescent scanning instrument, and the signals collecting of chemoluminescence method mainly detects by high-sensitive CCD detecting device, the shortcoming of these detectors be expensive, serviceable life is shorter, maintenance cost is high.In addition, for adapting to the detection of fluorescence method and chemoluminescence method, the material of biochip also has some limitation, can only select glass-chip or polymer chip without fluorescence background or luminescent substance, the preparation cost of such chip is higher, has greatly limited the application of biochip.And catalysis development process does not need expensive detecting instrument, testing result can visual direct qualitative judgement sample in the content of determinand, also can record gray-scale value by CCD, by calculating gray-scale value, draw the content of component to be measured in sample, greatly reduce testing cost.
Summary of the invention
For solve existing chip scanning method expensive, equipment is complicated, serviceable life is shorter, maintenance cost is high and the material of chip is required to high shortcoming, the invention provides a kind of transmission-type scanning method of biochip.
To achieve these goals, the present invention adopts following technical scheme: a kind of transmission-type scanning method of biochip, is characterized in that: comprise the following steps:
The first step: utilize scanner to scan under transmission-type scanning pattern; Before scanning, by the optical signal receiver (4) being placed under biochip, receive incident light (2), measure its light signal strength
i 0 ;
Second step: during scanning, incident light passes through, after chip solid phase substrate (1) and chip array point (5), to receive transmitted light (3) by optical signal receiver (4), measures optical signal transmissive intensity
i;light is after chip solid phase substrate (1) and chip array point (5), and light intensity produces decay, and attenuation ratio is
i/I 0 ;
The 3rd step: measure solid formation content
cwith corresponding attenuation ratio
i/I 0 , drawing standard curve
c-
i/I 0 ;
The 4th step: by a certain attenuation ratio
i/I 0 ' with typical curve
c-
i/I 0 comparison, determine the content of solid formation
c '.
The 5th step: according to the solid formation content obtaining
c 'with Sample Dilution multiple, calculate the content of each component to be measured in sample.
Before transmission-type scanning, by the mode of blank solid phase substrate background correction, carry out the impact of correcting chip solid phase substrate on light signal strength.
Described solid phase substrate material is to comprise transparent plastic, clear glass, hyaline membrane, transparent high polymer.
Described transparent high polymer comprises transparent porous plate.
Described porous plate comprises 24 orifice plates, 48 orifice plates, 96 orifice plates, 384 orifice plates.
Described solid formation is coloured sediment.
Beneficial effect: the present invention has realized the simple fast of biochip and quantitatively detected, and reduces testing cost simultaneously; With respect to flat reflective scanning, the present invention detects flat reflective to become stereo transmission formula, improves the sensitivity and the accuracy that detect.
Accompanying drawing explanation
Fig. 1 is the detection schematic diagram of the transmission-type scanning method of a kind of biochip of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
As shown in Figure 1, a kind of transmission-type scanning method of biochip, is characterized in that: comprise the following steps:
The first step: utilize scanner to scan under transmission-type scanning pattern; Before scanning, by the optical signal receiver (4) being placed under biochip, receive incident light (2), measure its light signal strength
i 0 ;
Second step: during scanning, incident light passes through, after chip solid phase substrate (1) and chip array point (5), to receive transmitted light (3) by optical signal receiver (4), measures optical signal transmissive intensity
i;light is after chip solid phase substrate (1) and chip array point (5), and light intensity produces decay, and attenuation ratio is
i/I 0 ;
The 3rd step: measure solid formation content
cwith corresponding attenuation ratio
i/I 0 , drawing standard curve
c-
i/I 0 ;
The 4th step: by a certain attenuation ratio
i/I 0 ' with typical curve
c-
i/I 0 comparison, determine the content of solid formation
c ';
The 5th step: according to the solid formation content obtaining
c 'with Sample Dilution multiple, calculate the content of each component to be measured in sample.
Before transmission-type scanning, by the mode of blank solid phase substrate background correction, carry out the impact of correcting chip solid phase substrate on light signal strength.
Described solid phase substrate material is to comprise transparent plastic, clear glass, hyaline membrane, transparent high polymer.
Described transparent high polymer comprises transparent porous plate.
Described porous plate comprises 24 orifice plates, 48 orifice plates, 96 orifice plates, 384 orifice plates.
Described solid formation is coloured sediment.
The content of the degree of light signal strength decay and the upper solid formation of chip respective array point is proportionate.
The foregoing is only the preferred embodiments of the present invention, be not restricted to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in claim scope of the present invention.
Claims (6)
1. a transmission-type scanning method for biochip, is characterized in that: comprise the following steps:
The first step: utilize scanner to scan under transmission-type scanning pattern; Before scanning, by the optical signal receiver (4) being placed under biochip, receive incident light (2), measure its light signal strength
i 0 ;
Second step: during scanning, incident light passes through, after chip solid phase substrate (1) and chip array point (5), to receive transmitted light (3) by optical signal receiver (4), measures optical signal transmissive intensity
i;light is after chip solid phase substrate (1) and chip array point (5), and light intensity produces decay, and attenuation ratio is
i/I 0 ;
The 3rd step: measure solid formation content
cwith corresponding attenuation ratio
i/I 0 , drawing standard curve
c-
i/I 0 ;
The 4th step: by a certain attenuation ratio
i/I 0 ' with typical curve
c-
i/I 0 comparison, determine the content of solid formation
c ';
The 5th step: according to the solid formation content obtaining
c 'with Sample Dilution multiple, calculate the content of each component to be measured in sample.
2. the transmission-type scanning method of a kind of biochip according to claim 1, is characterized in that: before transmission-type scanning, by the mode of blank solid phase substrate background correction, carry out the impact of correcting chip solid phase substrate on light signal strength.
3. the transmission-type scanning method of a kind of biochip according to claim 1, is characterized in that: described solid phase substrate material comprises transparent plastic, clear glass, hyaline membrane, transparent high polymer.
4. the transmission-type scanning method of a kind of biochip according to claim 3, is characterized in that: described transparent high polymer comprises transparent porous plate.
5. the transmission-type scanning method of a kind of biochip according to claim 4, is characterized in that: described porous plate comprises 24 orifice plates, 48 orifice plates, 96 orifice plates, 384 orifice plates.
6. the transmission-type scanning method of a kind of biochip according to claim 1, is characterized in that: described solid formation is coloured sediment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310577074.7A CN103592265B (en) | 2013-11-19 | 2013-11-19 | A kind of transmission-type scanning method of biochip |
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| CN201310577074.7A CN103592265B (en) | 2013-11-19 | 2013-11-19 | A kind of transmission-type scanning method of biochip |
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| CN103592265A true CN103592265A (en) | 2014-02-19 |
| CN103592265B CN103592265B (en) | 2016-09-14 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548179A (en) * | 2015-12-04 | 2016-05-04 | 深圳市赛尔生物技术有限公司 | Method and system for determination of biochip based on transmitted light or self luminescence |
| CN110927076A (en) * | 2019-11-14 | 2020-03-27 | 量准（上海）医疗器械有限公司 | Biological detection device and detection method adopting gold nanopore array chip |
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| JPH05157693A (en) * | 1991-12-03 | 1993-06-25 | Dainippon Printing Co Ltd | Instrument and method for measuring light transmittance of light shielding material |
| JP2000081367A (en) * | 1998-09-07 | 2000-03-21 | Nikon Corp | Light transmission optical member, its manufacturing method, its evaluation method, and light lithography device |
| CN1556408A (en) * | 2004-01-08 | 2004-12-22 | 张晓膺 | Immune turbidimetry for detection of apoliprotein M |
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2013
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Patent Citations (5)
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| JPH0436640A (en) * | 1990-05-31 | 1992-02-06 | Aloka Co Ltd | Absorbancy coeficient measuring instrument |
| JPH05157693A (en) * | 1991-12-03 | 1993-06-25 | Dainippon Printing Co Ltd | Instrument and method for measuring light transmittance of light shielding material |
| JP2000081367A (en) * | 1998-09-07 | 2000-03-21 | Nikon Corp | Light transmission optical member, its manufacturing method, its evaluation method, and light lithography device |
| CN1556408A (en) * | 2004-01-08 | 2004-12-22 | 张晓膺 | Immune turbidimetry for detection of apoliprotein M |
| CN102879374A (en) * | 2012-10-15 | 2013-01-16 | 无锡天授信息科技有限公司 | Laser-induced fluorescence Z-shaped total reflection detection cell device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548179A (en) * | 2015-12-04 | 2016-05-04 | 深圳市赛尔生物技术有限公司 | Method and system for determination of biochip based on transmitted light or self luminescence |
| CN110927076A (en) * | 2019-11-14 | 2020-03-27 | 量准（上海）医疗器械有限公司 | Biological detection device and detection method adopting gold nanopore array chip |
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| CN103592265B (en) | 2016-09-14 |
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