CN103585292B - A kind of mao Herba delphinii grandiflori extract of opening up is preparing the application in cancer therapy drug - Google Patents
A kind of mao Herba delphinii grandiflori extract of opening up is preparing the application in cancer therapy drug Download PDFInfo
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Abstract
The present invention relates to and a kind ofly open up a mao novelty teabag for Herba delphinii grandiflori extract, this novelty teabag is that exhibition hair Herba delphinii grandiflori extract is preparing the application in cancer therapy drug.Described exhibition hair Herba delphinii grandiflori extract is prepared as follows: to open up mao Herba delphinii grandiflori for raw material, first extract volatile oil, then extracts by different concentration ethanol, finally prepares by low-concentration hcl extraction with aqueous solution.Exhibition hair Herba delphinii grandiflori extract provided by the present invention is significant to preparing cancer therapy drug.
Description
Technical field
The invention belongs to biomedicine field, relate to a kind of Chinese medicinal material extract and preparing the application in cancer therapy drug, specifically relate to a kind of mao Herba delphinii grandiflori extract of opening up and preparing the application in cancer therapy drug.
Background technology
According in February, 2010 Ministry of Public Health statistics, annual global number of cancer deaths reaches 1,000 ten thousand.The reason of current global 1/4th human mortalities is caused by cancer, and prediction is gone down by current trend development, is owing to having suffered from cancer to the year two thousand fifty by there being the died of 1/2nd.The pernicious cancer morbidity of China, with annual 2.5-5% speed increment, has become the arch-criminal of serious harm human life.Higher with sickness rate such as pulmonary carcinoma, gastric cancer, hepatocarcinoma in the middle of various types of cancer.
It is required that the formation that blood vessel is normal structure organ betides function maintenance, and all diseases that it and human development lack of proper care and Different types of etiopathogenises causes are closely related.Tumor neovasculature is formed in tumorigenesis, transfer and recurrence and plays very important effect.As long as a series of research shows effectively Tumor suppression neovascularization, just can generate, shift and recurrence by Tumor suppression.In addition, the disease such as diabetes and rheumatic arthritis has blood vessel hyperplasia, its aberrant angiogenesis and tumor vessel closely similar.Therefore, angiogenic inhibitor also can be used for vascular proliferative disease such as treatment diabetes and rheumatic arthritis etc.In recent years, the research of people to tumor-blood-vessel growth mechanism deepens continuously, and medicine such as Avastin, Angiostatin etc. of many Antineoplastic angiogenesis are developed in succession, apply clinically.This kind of medicine not only may be used for the treatment of most of solid tumor, the prevention of tumor and the treatment of Malignancy can also be used for, simultaneously to the disease of other and associated angiogenesis as the prevention of diabetic renal papillary necrosis, rheumatic arthritis, psoriasis, hemangioma, atherosclerosis etc. and treatment, all there is certain theory and realistic meaning.
Tumor cell in tumor can be divided into two classes.One class is common tumor cell, and a class is tumor stem cell.Common tumor cell has quick division, and the sense of antagonism anticancer sensitivity, does not have the features such as self updating ability.Therefore common tumor cell can be dead after the certain algebraically of division.Tumor stem cell then has following features: remain static under normal circumstances, and not splitting status, insensitive to antineoplastic agent, has self updating ability, namely has the ability of Immortalization.In chemotherapy of tumors, a large amount of tumor cells is killed (because responsive to chemotherapeutic), but tumor stem cell can survive (because insensitive to chemotherapeutic).The recurrence of tumor is because chemotherapy of tumors medicine is invalid to tumor stem cell.The medicine effectively treating tumor stem cell is looked for be a current direction.The key of tumor eradication can not lie in and can kill common tumor cell, and be tumor eradication stem cell.
The history of Chinese medicine cancer is of long standing and well established, and existing Ramulus et folium taxi cuspidatae, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae), arsenicum, green tea, Ganoderma, Radix Ginseng, Herba Catharanthi Rosei, Radix Asparagi, Herba Scutellariae Barbatae, Radix Semiaquilegiae etc. are for clinical anticancer so far, achieve some good effects.For a long time, people apply Traditional Thinking, theory and method research and development anticancer herbal drug, although achieve progress, produce little effect.Chinese herbal medicine becomes first-selected cancer therapy drug not yet at home and abroad so far, and the anticancer therapeutic assistant officer of existing clinical Chinese herbal medicine needs to improve further.Therefore, research and development Chinese herbal medicine cancer therapy drug is the task of top priority of the pernicious cancer for the treatment of at present, and the chemical nature illustrating endogenous low molecule tumour-inhibitory substance is the key problem of this area research.
Exhibition hair Herba delphinii grandiflori is be ranunculaceae plant exhibition hair Herba delphinii grandiflori DelphiniumkamaonenseHuthvar.glabrescens (W.T.Wang) W.T.Wang and the dry aerial parts belonging to several plants together.6 ~ August gathers, and dries.Record in " Drug Standard of Ministry of Public Health of the Peoples Republic of China Tibetan medicine (first) ", standard number: WS3-BC-0088-95.Cool in nature, bitter in the mouth.There is heat clearing away, antidiarrheal dysentery, more effect of skin ulcer.For intestinal heat diarrhoea, dysentery; The external application open sore part.The common drug of the minority areas such as illiteracy is hidden for China.But about the basic research of exhibition hair Herba delphinii grandiflori is still very limited, the follow-up promotion and application of this medical material are restricted.Modern study shows, exhibition hair Herba delphinii grandiflori contains alkaloids composition; Exhibition hair Herba delphinii grandiflori pharmacological research has no report.
Domestic patent search result, has no exhibition hair Herba delphinii grandiflori Patents.
Above-mentioned document and patent etc., there is not yet the report of exhibition hair Herba delphinii grandiflori or the research of exhibition hair Herba delphinii grandiflori extract antitumaous effect.
Summary of the invention
A kind of mao Herba delphinii grandiflori extract of opening up is the object of the present invention is to provide to prepare the application in cancer therapy drug.
The present invention is achieved through the following technical solutions:
The present invention's exhibition hair used Herba delphinii grandiflori is ranunculaceae plant exhibition hair Herba delphinii grandiflori DelphiniumkamaonenseHuthvar.glabrescens (W.T.Wang) W.T.Wang and the dry aerial parts belonging to several plants together.
A kind of mao Herba delphinii grandiflori extract of opening up of the present invention is preparing the application in cancer therapy drug, and described exhibition hair Herba delphinii grandiflori extract prepares as follows:
(1) hair Herba delphinii grandiflori is opened up, pulverize, cross 10-50 mesh sieve, adopt supercritical carbon dioxide extraction method, extraction temperature 40 DEG C-50 DEG C, extracting pressure 20-30MPa, extraction time 1-2h, extraction volatile oil, collects volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 30%-75% alcoholic solution step (1) obtained as solvent, Extracting temperature 50 DEG C-95 DEG C, extraction time is 1-3 time, and each extraction time is 1-4 hour, each solvent load be the 6-15 of exhibition hair Herba delphinii grandiflori weight doubly; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 1%-5% hydrochloric acid solution as solvent, Extracting temperature 60 DEG C-80 DEG C, extraction time is 1-3 time, and each extraction time is 1-3 hour, and each solvent load is 10-15 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, exhibition hair Herba delphinii grandiflori extract C, wherein one or both or three kinds mix by a certain percentage, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Exhibition hair Herba delphinii grandiflori extract prepares as follows:
(1) hair Herba delphinii grandiflori is opened up, pulverize, cross 10-50 mesh sieve, adopt supercritical carbon dioxide extraction method, extraction temperature 40 DEG C-50 DEG C, extracting pressure 20-30MPa, extraction time 1-2h, extraction volatile oil, collects volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 30%-75% alcoholic solution step (1) obtained as solvent, Extracting temperature 50 DEG C-95 DEG C, extraction time is 1-3 time, and each extraction time is 1-4 hour, each solvent load be the 6-15 of exhibition hair Herba delphinii grandiflori weight doubly; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 1%-5% hydrochloric acid solution as solvent, Extracting temperature 60 DEG C-80 DEG C, extraction time is 1-3 time, and each extraction time is 1-3 hour, and each solvent load is 10-15 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Preferred exhibition hair Herba delphinii grandiflori extract prepares as follows:
(1) open up hair Herba delphinii grandiflori, pulverize, cross 30 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 45 DEG C, extracting pressure 25MPa, extraction time 1.5h, extraction volatile oil, collect volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 60% alcoholic solution step (1) obtained as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2.5 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 3% hydrochloric acid solution as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Exhibition hair Herba delphinii grandiflori extract of the present invention is preparing the application in cancer therapy drug, is especially preparing the application in medicines resistant to liver cancer, anti-lung-cancer medicament, drugs against colon cancer.
Exhibition hair Herba delphinii grandiflori exhibition hair Herba delphinii grandiflori extract A of the present invention, exhibition hair Herba delphinii grandiflori extract B, exhibition hair Herba delphinii grandiflori extract C are preparing the application in cancer therapy drug.
Exhibition hair Herba delphinii grandiflori extract A of the present invention, exhibition hair Herba delphinii grandiflori extract B, exhibition hair Herba delphinii grandiflori extract C are preparing the application in medicines resistant to liver cancer, anti-lung-cancer medicament, drugs against colon cancer.
The cancer therapy drug that exhibition hair Herba delphinii grandiflori extract of the present invention and chemical drugs or Chinese medicine or natural drug form.
The cancer therapy drug that exhibition hair Herba delphinii grandiflori extract A of the present invention, exhibition hair Herba delphinii grandiflori extract B, exhibition hair Herba delphinii grandiflori extract C and chemical drugs or Chinese medicine or natural drug form.
Exhibition hair Herba delphinii grandiflori extract of the present invention, by adding the various adjuvants that pharmaceutics allows, makes the peroral dosage forms such as the tablet on pharmaceutics, granule, capsule.
The each extract of exhibition hair Herba delphinii grandiflori of the present invention is to the inhibitory action of kinds of tumor cells.Experimental result shows, each extract of exhibition hair Herba delphinii grandiflori all has inhibitory action to a certain degree to human liver cancer cell BEL-7402, human liver cancer cell HepG-2, colon cancer cell HCT-8 and lung carcinoma cell A-549.The suppression ratio of exhibition hair Herba delphinii grandiflori extract to human liver cancer cell BEL-7402, human liver cancer cell HepG-2, colon cancer cell HCT-8 and lung carcinoma cell A-549 is respectively 72.61%, 43.86%, 61.24%, 68.88%.
Detailed description of the invention
Open up mao Herba delphinii grandiflori extract preparing the application in cancer therapy drug below by specific experiment example and embodiment be described further a kind of, but be not limited to the present invention.
Embodiment 1: the preparation of exhibition hair Herba delphinii grandiflori extract
(1) open up hair Herba delphinii grandiflori 15kg, pulverize, cross 30 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 45 DEG C, extracting pressure 25MPa, extraction time 1.5h, extraction volatile oil, collect volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 60% alcoholic solution step (1) obtained as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2.5 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 3% hydrochloric acid solution as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Embodiment 2: the preparation of exhibition hair Herba delphinii grandiflori extract
(1) open up hair Herba delphinii grandiflori 20kg, pulverize, cross 10 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 50 DEG C, extracting pressure 20MPa, extraction time 2h, extraction volatile oil, collect volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 30% alcoholic solution step (1) obtained as solvent, Extracting temperature 95 DEG C, extraction time is 1 time, and each extraction time is 4 hours, and each solvent load is 15 times of exhibition hair Herba delphinii grandiflori weight; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 5% hydrochloric acid solution as solvent, Extracting temperature 60 DEG C, extraction time is 3 times, and each extraction time is 1 hour, and each solvent load is 10 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Embodiment 3: the preparation of exhibition hair Herba delphinii grandiflori extract
(1) open up hair Herba delphinii grandiflori 18kg, pulverize, cross 50 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 40 DEG C, extracting pressure 30MPa, extraction time 1h, extraction volatile oil, collect volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 75% alcoholic solution step (1) obtained as solvent, Extracting temperature 50 DEG C, extraction time is 3 times, and each extraction time is 1 hour, and each solvent load is 6 times of exhibition hair Herba delphinii grandiflori weight; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 1% hydrochloric acid solution as solvent, Extracting temperature 80 DEG C, extraction time is 1 time, and each extraction time is 3 hours, and each solvent load is 15 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, obtain exhibition hair Herba delphinii grandiflori extract of the present invention.
Embodiment 4: the preparation of exhibition hair Herba delphinii grandiflori extract sheet
Example 1 opens up hair Herba delphinii grandiflori extract 345g, adds starch 105g, mixing, granulates, dry, sieves, adds microcrystalline Cellulose 35g, magnesium stearate 5g, and mixing, is pressed into 1000, must opens up hair Herba delphinii grandiflori extract sheet.
Embodiment 5: the preparation of exhibition hair Herba delphinii grandiflori extract particles
Example 2 opens up hair Herba delphinii grandiflori extract 345g, adds dextrin 95g, sucrose 65g, mixing, granulates, and dry, granulate, must open up hair Herba delphinii grandiflori extract particles.
Embodiment 6: the preparation of exhibition hair Herba delphinii grandiflori extract capsule
Example 3 opens up hair Herba delphinii grandiflori extract 345g, adds starch 85g, mixing, granulates, and dry, granulate, must open up hair Herba delphinii grandiflori extract capsule by encapsulated 1000.
Experimental example 1: the application of each extract of exhibition hair Herba delphinii grandiflori in inhibition tumor cell growth
By tumor cell (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, the human lung carcinoma cell A-549) trypsinization with 0.25% of logarithmic growth, then with containing the clear DMEM complete medium suspension cell of 10% (volumn concentration) fetal blood, make cell suspension, and respectively each tumor cell is diluted to 1.0X10
5individual/mL, then be seeded in 96 well culture plates, every hole 200uL, each tumor cell inoculates 6 holes respectively, in 37 DEG C, 5%CO
2incubator in cultivate 24h; Then incline each hole culture fluid.
The exhibition hair Herba delphinii grandiflori extract obtained by embodiment 1 method, exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, exhibition hair Herba delphinii grandiflori extract C (lot number is respectively 20110506,20110507,20110508,20110509) are dissolved in DMSO respectively, then dilute with DMEM complete medium, obtain the solution opening up each extract of hair Herba delphinii grandiflori, the final concentration of each extract of exhibition hair Herba delphinii grandiflori is 50ug/mL, wherein, the concentration of DMSO is 10% (volumn concentration).
Experimental group: each extract solution of exhibition hair Herba delphinii grandiflori is added above-mentioned tumor cell HepG-2 successively respectively, in the culture hole of BEL-7402, HCT-8, A-549, inoculation 3 holes separately, every hole adds 200ul; Matched group: add the DMEM complete medium containing 10% (volumn concentration) DMSO in the culture hole of above-mentioned each tumor cell, separately inoculation 3 holes, every hole adds 200ul; The culture plate of above each group is placed in 37 DEG C, 5%CO simultaneously
2incubator in cultivate 2 days.Within 2 days, backward every hole adds 20ul freshly prepared 5mg/ml tetramethyl azo azoles salt (MTT) solution, and at 37 DEG C, 5%C0
2condition under continue cultivate 4h; Carefully abandon supernatant, every hole adds 200ulDMSO, and room temperature places 15-20min, and every 5min vibration once; Microplate reader measures the optical density value (OD value) of each hole at wavelength 490nm place, by formulae discovery growth of tumour cell suppression ratio.
Suppression ratio (%)=(OD
contrast-OD
experiment)/OD
contrastx100%.
Three repetitions are established in experiment, and result is taken the mean.The inhibiting result of each extract of exhibition hair Herba delphinii grandiflori to people different tumor cell HepG-2, BEL-7402, HCT-8, A-549 is as shown in table 1.
The inhibitory action of each extract of hair Herba delphinii grandiflori to the different tumor cell of people opened up by table 1.
The inhibitory action experimental result of the exhibition each extract of hair Herba delphinii grandiflori to kinds of tumor cells shows, each extract of exhibition hair Herba delphinii grandiflori all has inhibitory action to a certain degree to human liver cancer cell BEL-7402, human liver cancer cell HepG-2, colon cancer cell HCT-8 and lung carcinoma cell A-549.The suppression ratio of exhibition hair Herba delphinii grandiflori extract to human liver cancer cell BEL-7402, human liver cancer cell HepG-2, colon cancer cell HCT-8 and lung carcinoma cell A-549 is respectively 72.61%, 43.86%, 61.24%, 68.88%.
Claims (5)
1. open up a mao preparation method for Herba delphinii grandiflori extract for one kind:
(1) open up hair Herba delphinii grandiflori, pulverize, cross 30 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 45 DEG C, extracting pressure 25MPa, extraction time 1.5h, extraction volatile oil, collect volatile oil, must open up hair Herba delphinii grandiflori extract A, the medicinal residues A after supercritical carbon dioxide extraction is for subsequent use;
(2) medicinal residues A concentration 60% alcoholic solution step (1) obtained as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2.5 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, obtain extracting solution B and medicinal residues B; Extracting solution B, reclaim ethanol, concentrate drying, must open up hair Herba delphinii grandiflori extract B;
(3) by the medicinal residues B that step (2) obtains, with concentration 3% hydrochloric acid solution as solvent, Extracting temperature 70 DEG C, extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of exhibition hair Herba delphinii grandiflori weight; Filter, merge extractive liquid, concentrate drying, must open up hair Herba delphinii grandiflori extract C;
(4) above-mentioned exhibition hair Herba delphinii grandiflori extract A, exhibition hair Herba delphinii grandiflori extract B, the mixing of exhibition hair Herba delphinii grandiflori extract C, must open up hair Herba delphinii grandiflori extract.
2. a kind ofly according to claim 1 open up a mao preparation method for Herba delphinii grandiflori extract, it is characterized in that the various adjuvants that described exhibition hair Herba delphinii grandiflori extract allows by adding pharmaceutics, making the tablet on pharmaceutics, granule or capsule.
3. open up hair Herba delphinii grandiflori extract A and prepare the application in cancer therapy drug, the preparation method of described exhibition hair Herba delphinii grandiflori extract A is: exhibition hair Herba delphinii grandiflori, pulverize, cross 30 mesh sieves, adopt supercritical carbon dioxide extraction method, extraction temperature 45 DEG C, extracting pressure 25MPa, extraction time 1.5h, extraction volatile oil, collect volatile oil, to obtain final product.
4. exhibition hair Herba delphinii grandiflori extract A according to claim 3 is preparing the application in cancer therapy drug, it is characterized in that described cancer therapy drug is selected from medicines resistant to liver cancer, anti-lung-cancer medicament, drugs against colon cancer.
5. exhibition hair Herba delphinii grandiflori extract A according to claim 3 is preparing the application in cancer therapy drug, it is characterized in that described exhibition hair Herba delphinii grandiflori extract A and chemical drugs or Chinese medicine composition use.
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| 我国翠雀属植物生物碱类化合物的研究进展;尹相博等;《黑龙江农业科学》;20130910(第9期);第135-137页 * |
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