CN103585143A - Application of formononetin in preparation of aspergillus fumigatus resistant medicament - Google Patents
Application of formononetin in preparation of aspergillus fumigatus resistant medicament Download PDFInfo
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- CN103585143A CN103585143A CN201310550616.1A CN201310550616A CN103585143A CN 103585143 A CN103585143 A CN 103585143A CN 201310550616 A CN201310550616 A CN 201310550616A CN 103585143 A CN103585143 A CN 103585143A
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- formononetin
- aspergillus fumigatus
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- antifungal
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Abstract
The invention relates to application of formononetin in aspergillus fumigatus prevention. The technical scheme of the invention provides application of the formononetin in preparation of a fungus infectious disease medicament. The fungus is aspergillus fumigatus. The compound in the invention is high in antifungal activity to various funguses on both the superficial part and the deep part. Compared with the conventional clinically applied antifungal medicament, the formononetin has the advantages of high efficiency, low toxicity, large antifungal spectrum and the like and can be used for preparing antifungal medicaments.
Description
Technical field
The present invention relates to a kind of compound application, specifically, is in Aspergillus fumigatus purposes about formoononetin.
Background technology
The position that fungal infectious disease is invaded human body according to fungus is divided into 4 classes: superficial fungi disease, dermatomycosis, subcutaneous mycosis and systemic mycoses; The former two is collectively referred to as superficial mycosis, and the latter two are called again deep mycosis.Fungal infectious disease is divided into following several according to fungal infection difference: candida albicans bacterium infectious disease, Candida parapsilosis bacterium infectious disease, Candida glabrata infectious disease, infection by Cryptococcus neoformans disease, Gypsum Fibrosum shape sporidiole bacteria infectious disease, trichophyton infectious disease, aspergillus fumigatus infection disease.
In recent years, extensive use along with broad ectrum antibiotic, corticosteroid and immunosuppressant, advanced Clinics is as the popularization of organ transplantation etc., AIDS's is popular, and the impact of tumor Radiotherapy chemotherapy, cause fungal infection particularly deep fungal infection significantly rise, deep fungal infection has now become the major disease main causes of death such as acquired immune deficiency syndrome (AIDS) and tumor.
Reported at present and clinically conventional nitrogen azole compounds have ketoconazole (Ketoconazole, KCZ), fluconazol (Fluconazole, FCZ), voriconazole (Voriconazole, VCZ), itraconazole (Itraconazole, ICZ), amphotericin B (Amphotericin B, AMB) etc., but these compounds still exist, and above-mentioned toxic and side effects is large, narrow antimicrobial spectrum, easily produce the defects such as drug resistance, for example bring into play the required dosage of drug effect larger, thereby can produce larger toxic and side effects to human body.
Formononetin (Formononetin), has another name called formoononetin, is a kind of Isoflavone compounds, comes from inflorescence and the wounded in the battle branch and leaf of leguminous plant Herba Trifolii Pratentis (Trifolium Pratense L).Its molecular formula is C
16h
12o
4, molecular weight is 268.26.Research shows, formononetin has blood lipid metabolism in control agent, suppresses the pharmacological activities such as the atherosis and estrogen-like effects of Liver fatty deposition and prevention of arterial.But in infectious disease application, have no report about formononetin.
Summary of the invention
The object of the invention is for deficiency of the prior art, provide a kind of formoononetin in Aspergillus fumigatus purposes.
For achieving the above object, the technical scheme that the present invention takes is: the application of a kind of formoononetin in preparing fungal infectious disease medicine, described fungus is Aspergillus fumigatus.
The invention has the advantages that: compound of the present invention has good antifungal activity to various superficial parts and deep fungal, compare with the antifungal drug of existing clinical practice to there is the advantages such as efficient, hypotoxicity, anti-fungus spectra be wide, can be used for preparing antifungal drug.
The specific embodiment
Below the specific embodiment provided by the invention is elaborated.
Embodiment 1
Below the specific embodiment provided by the invention is elaborated.
(1) experimental technique: adopt conventional In Vitro Bacteriostasis experimental technique (to refer to: Antimicrob Agents Chemother 1995,39 (5): 1169)
1. materials and methods
(1) testing compound of the present invention: formononetin has another name called formoononetin (Formononetin), its molecular formula is C
16h
12o
4, molecular weight is 268.26.
Experimental strain is as follows:
This experiment has selected following 8 kinds of common human body cause illness's standard fungal bacterial strains as screening object:
(2) test method
Bacteria suspension preparation: above-mentioned fungus is cultivated 16 hours through 35 ℃ of YEPD fluid mediums, twice activation, with blood cell counting plate counting, adjusts bacteria concentration to 1 * 10 with RPM1640 fluid medium
4～1 * 10
5individual/mL.
Medicinal liquid preparation: get testing compound of the present invention and be dissolved in dimethyl sulfoxine, be made into the medicine storage liquid of 8.0mg/mL, be diluted to 640 μ g/mL with RPM1640 before experiment.
Inoculation: get drug sensitive plate, add RPMI RPMI-1640 200 μ l in No. 1 hole of every row, make blank; No. 12 hole adds bacterium liquid 200 μ l to be measured, makes negative control; 2 ~ No. 11 holes of the every row of drug sensitive plate add respectively bacterium liquid 180 μ l, fully mix, and make the final drug level in each hole be respectively 64,32,16,8,4,2,1,0.5,0.25 and 0.125 μ g/ml, and in each hole, DMSO content is all lower than 1%; Positive control, not containing medicine, is made in No. 12 holes.Control drug is fluconazol (FCZ), itraconazole (ICZ), voriconazole (VCZ), ketoconazole (KCZ), terbinafine (TBR), amphotericin B (AMB).
Cultivate and detect: establishing positive control hole optical density value (OD value) is 100%, the optical density value of take is minimal inhibitory concentration value (MIC than positive control hole lower than 80% lowest drug concentration
80).
(2) experimental result
In Vitro Bacteriostasis experimental result is in Table 2.
Note: KCZ. ketoconazole, FCZ. fluconazol, VCZ. Wo Likang azoles, ICZ. itraconazole, TRB. terbinafine, AMB amphotericin
Above-mentioned experimental result shows that compound of the present invention has good antifungal activity, and compound is all far better than fluconazol to the vitro inhibition activity of selected fungus, illustrates that the compounds of this invention can be used for preparing the medicine of anti-fungal infection.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the inventive method; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection scope of the present invention.
Claims (1)
1. the application of formoononetin in preparing fungal infectious disease medicine, described fungus is Aspergillus fumigatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310550616.1A CN103585143A (en) | 2013-11-08 | 2013-11-08 | Application of formononetin in preparation of aspergillus fumigatus resistant medicament |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310550616.1A CN103585143A (en) | 2013-11-08 | 2013-11-08 | Application of formononetin in preparation of aspergillus fumigatus resistant medicament |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103585143A true CN103585143A (en) | 2014-02-19 |
Family
ID=50075597
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310550616.1A Pending CN103585143A (en) | 2013-11-08 | 2013-11-08 | Application of formononetin in preparation of aspergillus fumigatus resistant medicament |
Country Status (1)
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| CN (1) | CN103585143A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926960A (en) * | 2017-12-04 | 2018-04-20 | 刘志伟 | A kind of bactericidal composition of isothiazolone-containing |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552241A (en) * | 2012-01-06 | 2012-07-11 | 南京中医药大学 | Application of formononetin in preparing medicine for preventing and treating airway inflammation and asthma |
-
2013
- 2013-11-08 CN CN201310550616.1A patent/CN103585143A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552241A (en) * | 2012-01-06 | 2012-07-11 | 南京中医药大学 | Application of formononetin in preparing medicine for preventing and treating airway inflammation and asthma |
Non-Patent Citations (2)
| Title |
|---|
| 曾虹燕等: "红车轴草有效成分的研究进展", 《中草药》, vol. 32, no. 2, 31 December 2011 (2011-12-31), pages 189 - 190 * |
| 邓丽: "甘草渣中黄酮类化合物的提取纯化、分离鉴定及其抑菌活性研究", 《中国优秀硕士学位论文全文数据库 工程科技Ι辑》, 15 October 2011 (2011-10-15), pages 37 - 45 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926960A (en) * | 2017-12-04 | 2018-04-20 | 刘志伟 | A kind of bactericidal composition of isothiazolone-containing |
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Application publication date: 20140219 |