CN103583544A - Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil - Google Patents
Application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil Download PDFInfo
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Abstract
The invention provides an application of 1% isothiazolinone.2% methylene bisthiocyanate missible oil in the prevention and treatment of bacterial fruit blotch, and 1% isothiazolinone.2% methylene bisthiocyanate missible oil, and belongs to the biotechnical field. The optimum use concentration of the 1% isothiazolinone.2% methylene bisthiocyanate missible oil in the field spray prevention and treatment and the optimum soup concentration, the optimum processing time and the like used for processing infected seeds are obtained through the indoor toxicity test, seed processing and the field prevention and treatment in the invention, so the germination percentage of seeds can be improved, the growth of roots can be promoted, a technical support is provided for the scientific and safe use of the missible oil, and a theoretical basis is provided for the prevention and treatment of the fruit blotch.
Description
Technical field
The invention belongs to biological technical field, relate to application and this medicament of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil in control Acidovorax Avenae Subsp. Citrulli.
Background technology
Melon bacterial fruit blotch (bacterial fruit blotch, BFB) is that multinational and a plurality of provinces of China, a kind of crushing bacteriosis ， world on ground family crop all have generation, belongs to worldwide quarantine disease.Its cause of disease is watermelon acidophil (Acidovorax citrulli Schaad et al., 2008).1998, Chinese scholar Zhang Rongyi Deng Hainan Province find and reported first this disease, its disease symptom is described in detail.In succession there is this disease in the province such as Xinjiang of China, Hainan, Henan, Fujian, the Inner Mongol, Shandong, Jilin, Heilungkiang in recent years.
This disease is typical seed-borne disease, and pathogen can survive 19 years on seed, by seed, carries out long-distance communications.This germ, except infecting watermelon and muskmelon, can also be infected the ground family crops such as pumpkin, cucumber, once occur, will bring serious economic loss.And contaminated seed causes plant in Seedling Stage, strain phase and fruit morbidity.Seed in field morbidity fruit has higher bacterial bearing rate.Therefore obtaining safely and effectively seed disinfectant prevents and treats this disease and seems particularly important.
Summary of the invention
According to the demand in above-mentioned field and deficiency, the invention provides the application of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil in control melon bacterial fruit blotch, and carried out Toxicity Determination, seed treatment and field controling test.The 1% best working concentration of isothiazolinone 2% 2 sulphur cyanogen methane missible oil medicament field spray control and the best liquor strength of processing infected seed, processing time etc. have been grasped, for can science on producing, this medicament of use of safety provides technical support, the control for fruit blotch simultaneously provides theoretical foundation.
Technical scheme of the present invention is as follows:
The application of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil in control melon bacterial fruit blotch.
Described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil extension rate is 50-1000 times.
The occupation mode of described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is for processing infected seed or spraying blade.
When for the treatment of infected seed, described extension rate is 50-200 times.
Described extension rate is 200 times.
The mode that described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is processed infected seed is 0.5-1.5 hour/time, is less than 0.5 hour control efficiency poor, and being greater than 1.5 hours can affect seed sprouting; And process infected seed, within 0.5-1.5 hour/time, substantially can meet the requirement that prevents and treats melon bacterial fruit blotch.
The mode that described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is processed infected seed is 1 hour/time.
When spraying blade, described extension rate is 1000 times.
For preventing and treating a medicament for melon bacterial fruit blotch, it is characterized in that, this medicament is dilution 50-1000 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil doubly.
The occupation mode of described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is for processing infected seed or spraying blade.
At present, the control of fruit blotch mainly lays particular emphasis on seed treatment and field Agro-chemicals control.The use such as the Zhao Tingchang 3% salt acid treatment Hami melon seed 1min that carries disease germs, sowing after adding 600 times of liquid seed soakings of auspicious agriculture and spend the night with 47% again after washing, has good control efficiency to preventing and treating melon bacterium fruit blotch.The for army building grade of fourth processed seed 15min with 2%～8% hydrogen peroxide, and not only bactericidal effect is good, can also improve the germination rate of seed.The researchs such as Wang Shuan show, the rotten clean seed control efficiency of processing for No. 1 of 1% formalin and fruit is respectively 97.6% and 94.9%, 72% streptomycin 720mg/L and processes seed control efficiency 76.8%.The researchs such as Niu Qingwei show, 40% 100 times, formaldehyde liquid is processed seed 1h, and seedling is had to good control efficiency.Field Agro-chemicals control is mainly in the majority with agricultural streptomycin and copper agent.Although the copper necessary trace element that is plant growth, the excessive of copper certainly will bring harm to plant, causes plant to occur poisoning phenomenon, and blade thickness is become fragile.
The control diversification of medicament and the rotation of medicament are used alternatingly, and not only in disease control, can slow down drug-fast generation, can also alleviate the pollution of chemical agent to environment.In the present invention, 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is gone up conventional seed treatment medicament and agricultural streptomycin sulphate and tiger glue adipic acid copper with production and is compared, that infected seed is processed, spraying control in seedling stage all has good effect, and carry out seed treatment, can improve the germination rate of seed, promote the growth of root.Therefore this medicament awaits becoming the first-selected medicament of producing upper control melon bacterial fruit blotch and seed treatment.
The present invention selects 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil and 4 kinds of contrast medicaments of preparation, the melon bacterial Acidovorax avenae subsp of take has carried out Toxicity Determination as target, and compared under different medicaments and variable concentrations and processed seed, the control efficiency of plant spraying to fruit blotch, chemicals treatment is on the germination rate of seed, the long impact of root.Result shows: 1% 200 times of isothiazolinone 2% 2 sulphur cyanogen methane missible oil liquid is processed infected seed 1h control efficiency and reached 89.9%, 1000 times of liquid control efficiency of spraying seedling stage reaches 75.2%, two kind and processes control efficiency and produce respectively the preventive effect high 32% and 2.3% of upper 72% conventional agricultural streptomycin sulphate.200 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil carry out seed treatment, improve the germination rate of seed, promote the growth of root.
Accompanying drawing explanation
Fig. 1 is Toxicity Determination result;
Fig. 2 is the impact that chemicals treatment is carried disease germs on the surface of the seed;
Fig. 3 is the impact of chemicals treatment on the muskmelon seeds that carries disease germs;
Fig. 4 is the impact of chemicals treatment on the watermelon seed that carries disease germs;
Fig. 5 is plant poisoning result after chemicals treatment (C and D are respectively the clear water contrast on watermelon and muskmelon);
Fig. 6 is control efficiency after seed chemicals treatment;
Embodiment
It is in order further to understand better the present invention that following embodiment is provided; be not limited to described embodiment; content of the present invention and protection domain are not construed as limiting; anyone under enlightenment of the present invention or by the present invention, combines with the feature of other prior aries and any and the present invention of drawing is identical or akin product, within all dropping on protection scope of the present invention.
Unreceipted specific experiment step or condition person in embodiment, can carry out according to the operation of the described normal experiment step of the document in this area or condition.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional reagent product of commercial acquisition.
Material reagent:
Strains tested: the watermelon acidophil strains A ac-5 that preserve in this laboratory, medium: King ' s B(KB) medium, water agar, for planting experimentally son: watermelon seed (vegetables center, Beijing City Agriculture and Forestry Institute provides), muskmelon seeds (Vegetable & Flower Inst., Chinese Academy of Agriculture Science provides) reagent agent: 1% isothiazoline copper 2% 2 sulphur cyanogen methane missible oil for purchased from the isothiazolinone of Beijing chemical reagents corporation and two sulphur cyanogen methane missible oil according to the ratio mixed preparing of the dimethylbenzene of 1% isothiazolinone and 5% 2 sulphur cyanogen methane missible oil and 97%, 72% agricultural streptomycin sulphate SPX(Huabei Pharmaceutic Co., Ltd), 30% tiger glue adipic acid copper SC(Plant Protection institute, Chinese Academy of Agricultral Sciences Langfang agricultural chemicals pilot plant), formaldehyde (Xilong Chemical Co., Ltd) and HCL(Beijing Chemical Plant).
Embodiment 1.
1. Toxicity Determination
Toxicity Determination adopts inhibition zone method.Choose Acidovorax avenae subsp list colony inoculation in liquid KB medium, 28 ℃, 200rpm/min constant temperature culture 24h, OD
600≈ 0.3(10
8cfu/mL).When medium temperature drops to 50 ℃ of left and right, bacterium liquid is added to (ratio of bacterium liquid and medium is 1:40) in medium, make flat board.Diameter 7mm sterilizing filter paper is placed in to flat board.By the reagent agent of variable concentrations, get 10uL and be added drop-wise in filter paper.Sterile water contrasts, and each is processed 3 times and repeats.28 ℃, after constant temperature culture 48h, antibacterial circle diameter size (see figure 1) is measured in right-angled intersection.
2. interpretation of result
2.1. indoor virulence
Toxicity test the results are shown in Table 1.As can be seen from Table 1, best for the fungistatic effect of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil in 5 kinds of medicaments of examination, the average antibacterial circle diameter of each concentration is at 22.1～39.0mm, and along with the diameter that increases inhibition zone of concentration increases; Each concentration antibacterial circle diameter of formaldehyde is at 9.1～38.6mm; The fungistatic effect of 72% agricultural streptomycin sulphate is only second to formaldehyde, and the average antibacterial circle diameter of each concentration is at 13.0～25.9mm; Each concentration inhibition zone scope of HCl is at 9.4～13mm, and the inhibition zone of 30% tiger glue adipic acid copper not obvious (Fig. 1).
The fungistatic effect of table 1. reagent agent to Acidovorax Avenae Subsp
2.2.1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil missible oil Virulent Analysis
The virulence regression equation of the medicaments such as 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is in Table 2.In reagent agent, formaldehyde and HCl have stronger volatility, although there is apparent inhibition zone on flat board, but it is low that its correlation coefficient is compared with 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil and 72% agricultural streptomycin sulphate, illustrates that two kinds of liquids exist error and certain limitation (in Table 2) when doing Toxicity Determination.The EC of 72% agricultural streptomycin sulphate and 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil
50be respectively 326061.8mg/L and 8943.3mg/L.The EC of 72% agricultural streptomycin sulphate
50compare and significantly improve with experimental results such as Wang Shuan, its EC
50be 36.3 times of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, not only improved cost accounting and caused serious antibiotic to pollute to environment.Low consumption, the high control efficiency of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil play a multiplier effect surely to preventing and treating melon fruit blotch.Therefore, this research thinks that 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil has broad application prospects.
The Virulent Analysis of table 2.4 kind of medicament to BFB
Embodiment 2.
1. seed treatment
Aac-5 bacterium liquid is adjusted to 10
8cfu/mL, mixes with the ratio of muskmelon seeds 1g, bacterium liquid 2mL, soaks 1h, dries up seed in superclean bench.Reagent agent, concentration for the treatment of and time, after chemicals treatment, clear water rinsed seed 3 times in Table 4.Seed is placed on water agar plate, will after the filter paper immersion of sterilizing, be covered on seed.50 seeds of every ware, repeat for 3 times.In 28 ℃ of vernalization, the germination rate of 16h " Invest, Then Investigate " seed, measures root long after 48h, with this, judges the impact of chemicals treatment on seed sprouting.By the remaining planting seed of same treatment, in sterilized soil, each is processed 3 times and repeats, and each repeats 15 young plants.Clear water compares.5d investigation cotyledon morbidity number after emerging, the preventive effect of calculating seed treatment, carries out significance analysis with DPS software.
Control efficiency (%)=[1-(processing the incidence of disease/contrast incidence of disease)] * 100.
Simultaneously, each repeats to get 5 seeds, single seed is in sterilized 1.5mL centrifuge tube, add 1mL sterile water, be placed in 4 ℃ and place after 24h, after concussion, draw 100uL leaching liquor and coat the KB flat board that is added with Amp, after 24h, add up the clump count on flat board, with the bactericidal effect of clear and definite each drug concentration, test repeats 3 times.The infected seed of usining does not carry out chemicals treatment as positive control (CK1); Not connecing the sub-clear water of bacterial classification processes as negative control (CK2).The surface of the seed quantitative statistics: the C that carries disease germs represents the average clump count (individual/plate) of germ on different chemicals treatment rear plates.Counting standard ,-: C=0; +: 0 < C≤10; ++: 10 < C≤100; +++: 100 < C≤500, can see inventory bacterium colony; ++++: C > 500, bacterium colony covers with whole flat board.And adopt identical method to process the watermelon seed that connects bacterium.
2. interpretation of result-infected seed is processed
After 2.1 chemicals treatment infected seeds, the bacterial bearing rate of the surface of the seed
We carry out chemicals treatment to the watermelon seed carrying disease germs and muskmelon seeds, and after chemicals treatment, the surface of the seed Carriage is as shown in Fig. 2 and table 3.200 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, 100 times of liquid and 50 times of liquid, 100 times of liquid of 40% formaldehyde, 3%HCL and 200 times of liquid of 30% tiger glue adipic acid copper, 100 times of liquid and 50 times of liquid are processed on rear plates without germ bacterium colony, and the surface of the seed viable bacteria rate is 0%; And 32.7 of average clump counts on 1000 times of liquid flat boards of 72% agricultural streptomycin sulphate, positive control CK1(infected seed clear water is processed) bacterium colony joins together on flat board.Blank CK2(does not connect the sub-clear water of bacterial classification and processes) on flat board without bacterium colony.Though tiger glue adipic acid copper does not have inhibition zone when doing bacteriostatic test, but after seed treatment, leaching liquor is coated with dull and stereotypedly, and on flat board, control efficiency illustrates that this medicament has some limitations when employing inhibition zone method carries out Toxicity Determination without bacterium colony and after emerging.
Table 3. seed chemicals treatment is on the surface of the seed impact of carrying disease germs
Note * CK1 positive control (processing of infected seed clear water); CK2 blank (not connecing the sub-clear water of bacterial classification processes).
The impact that 2.2 chemicals treatment are germinateed on infected seed
Medicament on the impact such as seed sprouting in Table 4 and Fig. 3.1% 200 times of isothiazolinone 2% 2 sulphur cyanogen methane missible oil liquid is processed after seed, and the germination rate of seed, emergence rate and control efficiency are respectively: 98.7%, 100% and 89.9%, and root is long is 45.76mm.All be significantly higher than CK1 and other 4 kinds contrast medicaments.1% 200 times of isothiazolinone 2% 2 sulphur cyanogen methane missible oil liquid is processed seed, and its germination rate and average emergence rate improve respectively 1.4% and 20% compared with CK1, and the growth of root is had to good facilitation.1% isothiazolinone 2% 2 100 times of liquid of sulphur cyanogen methane missible oil and 50 times of liquid are processed after seed, control efficiency is better than 200 times of liquid, but after two kinds of concentration are processed seed, the root length of seed is had a certain impact, after wherein 50 times of liquid are processed, the germination rate of seed reduces by 1.3% compared with the control.Therefore, from the viewpoint of economy, environmental protection and delay pesticide resistance generation etc., build and produce the upper 200 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil that use and process seeds.
The impact of table 4. chemicals treatment on the muskmelon seeds that carries disease germs
* note: CK1 (positive) connects bacterium; CK2(is negative): clear water.
On the basis of processing at muskmelon seeds, select 200 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, 1000 times of liquid of 72% agricultural streptomycin sulphate, 100 times of liquid of 40% formaldehyde and 3%HCL to process respectively carry disease germs watermelon seed 1h, 1h, 30min and 5min.By Fig. 4 and table 5, found out, although 200 times of liquid of the 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil that the present invention selects are processed after seeds the growth of root inhibitory action slightly, but the result of variance analysis can be found out from table 5, no matter its germination rate is process seed or compare with contrasting in this experiment with producing 1000 times of liquid of upper 72% conventional agricultural streptomycin sulphate, and germination rate is close.In sum, from above-mentioned data: 200 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, 1000 times of liquid of 72% agricultural streptomycin sulphate, 100 times of liquid of 40% formaldehyde and 3%HCL process seed control melon bacterial fruit blotch, and the optimization process time is once: 1h, 1h, 30min and 5min effect are better.
The impact of table 5. chemicals treatment on the watermelon seed that carries disease germs
* note A and represent that clear water rinses after chemicals treatment; B represents not clean after chemicals treatment;
1. spraying concentration detects
The agricultural streptomycin sulphate of reagent agent 72%, 30% tiger glue adipic acid copper and 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, all arrange 5 liquor strengths: 1200 times of liquid, 1000 times of liquid, 800 times of liquid, 500 times of liquid and 200 times of liquid.Sparge muskmelon seedling and watermelon seedling, each concentration repeats for 4 times, and each repeats 15 young plants, clear water contrast, 7d " Invest, Then Investigate " poisoning situation.
2. results and analysis
2.1 test result analysis
The agricultural streptomycin sulphate of reagent agent 72%, tiger glue adipic acid copper and 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil arrange 5 concentration and spray after watermelon and muskmelon seedling, and the drug resistance of plant performance is different.3 kinds of medicaments spray the equal healthy growth of plant after watermelon and muskmelon blade when 1000 times of liquid, grow fine, the same with contrast.3 kinds of medicaments affect watermelon and Muskmelon Plants growth when 800 times of liquid, 500 times of liquid and 200 times of liquid: wherein 1% isothiazolinone 2% 2 500 times of liquid of sulphur cyanogen methane missible oil and 200 times of liquid have obvious impact to the growth of watermelon and Muskmelon Plants, 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil of 200 times of liquid shows as growing point necrosis on watermelon plant, growth retardation (Fig. 5 A), Muskmelon Plants leaf-shrinkage, leaf margin yellow (Fig. 5 B), is therefore used the medicine liquid spray control fruit blotch effect of 1000 times better.
Embodiment 4.
1. field efficiency test
On the basis of phytotoxicity test, choose 1000 times of liquid of 72% agricultural streptomycin sulphate, 1000 times of liquid of 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil, 1000 times of liquid of 30% tiger glue adipic acid copper to spray inoculation Aac-5 bacterium liquid (OD
600≈ 0.3) muskmelon seedling and the processing of spraying of watermelon seedling, each is processed 4 times and repeats, each repeats 15 young plants; Clear water contrast.Respectively at processing initial stage and the final investigation of processing disease index, according to following grade scale, add up disease index: 0 grade: seedling does not have disease symptom; 1-4 level is respectively hypocotyl, one or two cotyledon lesion area accounts for 25%, 26%～50%, 51%～75% and 76%～100% of blade area; 5 grades: cotyledon is all fallen ill, hypocotyl is rotted, whole strain lodging.Calculate control efficiency.Data result carries out variance analysis.
Control efficiency (%)=[the final disease index-processing initial stage disease index in the final disease index-treatment region initial stage disease index/check plot of 1-(treatment region)] * 100
2. interpretation of result
2.1 control efficiency test result analysis
After the muskmelon seeds chemicals treatment of carrying disease germs, control efficiency is as shown in table 4 and Fig. 6: 1% 200 times of isothiazolinone 2% 2 sulphur cyanogen methane missible oil liquid is processed after muskmelon seeds, and control efficiency reaches 89.9%.40% 100 times, formaldehyde liquid is processed muskmelon seeds, control efficiency reaches 88.8%, 3%HCL seed treatment preventive effect and reaches 200 times of liquid of 87.4%, 30% tiger glue adipic acid copper and process muskmelon seeds preventive effects and reach 86.7%, 72% 1000 times of agricultural streptomycin sulphates liquid is processed muskmelon seeds, and preventive effect reaches 57.9%.In the inoculation muskmelon of Aac-5 and the efficiency test of watermelon seedlings, 3 kinds of 1000 times of liquid control efficiency significant differences of chemical agent (in Table 6).1% isothiazolinone 2% 2 sulphur cyanogen methane EC effect 75.8%; The control efficiency 72.9% of 72% agricultural streptomycin sulphate is close with the result of study of Wang Shuan etc.; 30% tiger glue adipic acid copper control efficiency reaches 71.8%.
Table 6. field spray control efficiency
By specific embodiment, explain the present invention above, but the invention is not restricted to this.Equivalent transformation or modification that all Spirit Essences according to the present invention are done, within all should being encompassed in protection scope of the present invention.
Claims (10)
- The application of 1.1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil in control melon bacterial fruit blotch.
- 2. application according to claim 1, is characterized in that, described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil extension rate is 50-1000 times.
- 3. application according to claim 2, is characterized in that, the occupation mode of described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is for processing infected seed or spraying blade.
- 4. application according to claim 3, is characterized in that, when for the treatment of infected seed, described extension rate is 50-200 times.
- 5. application according to claim 4, is characterized in that, described extension rate is 200 times.
- 6. application according to claim 5, is characterized in that, the mode that described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is processed infected seed is 0.5-1.5 hour/time.
- 7. application according to claim 5, is characterized in that, the mode that described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is processed infected seed is 1 hour/time.
- 8. application according to claim 3, is characterized in that, when when spraying blade, described extension rate is 1000 times.
- 9. for preventing and treating a medicament for melon bacterial fruit blotch, it is characterized in that, this medicament is dilution 50-1000 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil doubly.
- 10. medicament according to claim 9, is characterized in that, the occupation mode of described 1% isothiazolinone 2% 2 sulphur cyanogen methane missible oil is for processing infected seed or spraying blade.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109452291A (en) * | 2018-02-28 | 2019-03-12 | 中国农业科学院植物保护研究所 | A method of prevention and treatment cucumber bacterial angular leaf spot |
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| JPH05163107A (en) * | 1991-12-12 | 1993-06-29 | Shinto Paint Co Ltd | Industrial germicidal composition |
| CN1236542A (en) * | 1999-05-04 | 1999-12-01 | 王兴民 | Application of isothiazolinone derivatives |
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2013
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH05163107A (en) * | 1991-12-12 | 1993-06-29 | Shinto Paint Co Ltd | Industrial germicidal composition |
| CN1236542A (en) * | 1999-05-04 | 1999-12-01 | 王兴民 | Application of isothiazolinone derivatives |
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| Title |
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| 廖富荣等: "进境西瓜种子中西瓜细菌性果斑病菌的检测鉴定", 《植物保护》, vol. 36, no. 6, 31 December 2010 (2010-12-31), pages 135 - 138 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109452291A (en) * | 2018-02-28 | 2019-03-12 | 中国农业科学院植物保护研究所 | A method of prevention and treatment cucumber bacterial angular leaf spot |
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