CN103583517B - Attractant for luring and gathering dichocrocis punctiferalis and bait - Google Patents
Attractant for luring and gathering dichocrocis punctiferalis and bait Download PDFInfo
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- CN103583517B CN103583517B CN201310594690.3A CN201310594690A CN103583517B CN 103583517 B CN103583517 B CN 103583517B CN 201310594690 A CN201310594690 A CN 201310594690A CN 103583517 B CN103583517 B CN 103583517B
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Abstract
The invention discloses an attractant for luring and gathering dichocrocis punctiferalis and a bait, and belongs to the field of preparation of the attractant for dichocrocis punctiferalis. The attractant is prepared from compounds including alpha-pinene, camphene, sabinene and beta-pinene in a mixing mode, all the compounds are volatile active compounds released by a main host plant (Chinese chestnut) of dichocrocis punctiferalis in the fields. The attractant is prepared from the volatile active compounds with a function of luring dichocrocis punctiferalis in the mixing mode, can lure and gather a lot of dichocrocis punctiferalis female moths and a small number of male moths, can be applied to monitoring and prevention of the population dynamics of dichocrocis punctiferalis in chestnut orchards, is economical and effective and free from environmental pollution.
Description
Technical field
The present invention relates to the attractant of dichocrocis punctiferalis, particularly relate to a kind of lure collection dichocrocis punctiferalis attractant and lure core.
Background technology
Dichocrocis punctiferalis Conogethes punctiferalis (Guen é e) is under the jurisdiction of Lepidoptera, Pyralidae, and host plant comprises more than 40 kind of fruit tree, forest and the crops such as Chinese chestnut, peach, corn, is a kind of omnivorousness, borer pest.Harm is taken food into fruit inside owing to eating into very soon after larvae hatch, larval phase is caused to be prevented and treated very difficult, and apply insect sex pheromone control adult have selectivity strong, efficient, pollution-free, do not injure the advantages such as beneficial insect, the requirement of agricultural sustainable development can be met.Therefore, the monitoring of application pheromones and control dichocrocis punctiferalis have become the emphasis that recent domestic is paid close attention to.But single dependence insect sex pheromone only has gathering effect to male moth, but have no attracting action for seeking the female moth that host plant lays eggs, therefore, field gathering effect is had a greatly reduced quality.
Summary of the invention
The technical problem to be solved in the present invention is to provide and a kind ofly lures the attractant of collection dichocrocis punctiferalis and lure core, effectively can lure collection dichocrocis punctiferalis female moth, thus solve the existing attractant problem bad to dichocrocis punctiferalis female moth attractant effect.
For solving the problems of the technologies described above, the invention provides a kind of attractant luring collection dichocrocis punctiferalis, this attractant is formed by compound australene, amphene, sabinene and nopinene mixture, and each compound is all volatile active compounds that dichocrocis punctiferalis main host plants plant (Chinese chestnut) field discharges.
The present invention also provide a kind of lure collection dichocrocis punctiferalis lure core, this lures core to be with green rubber plug for carrier, and in described carrier, injection adds the attractant of described claim 1 or 2, and described attractant is infiltrated enter described carrier inside to be formed lure core.
Beneficial effect of the present invention is: this attractant forms to have the reactive compound mixture of luring dichocrocis punctiferalis effect, the a large amount of female moth of dichocrocis punctiferalis of collection and a small amount of male moth can be lured, not only can be applied to Li Yuan dichocrocis punctiferalis population occurrence dynamics Supervise prevention and cure, and economical and effective, non-environmental-pollution.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawings can also be obtained according to these accompanying drawings.
The accumulation luring core to pass in time of attractant that Fig. 1 provides for the carrying embodiment of the present invention lures moth curve;
Fig. 2 lures core day total induced moth number curve for the attractant that provides of the carrying embodiment of the present invention.
Embodiment
Carry out clear, complete description to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on embodiments of the invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to protection scope of the present invention.
Embodiment one
The embodiment of the present invention provides a kind of attractant luring collection dichocrocis punctiferalis, and this attractant is formed by compound australene, amphene, sabinene and nopinene mixture, and the consumption of each compound is preferably in mass ratio: australene 28, amphene 9, sabinene 37, nopinene 26; Each compound is all volatile active compounds of dichocrocis punctiferalis main host plants plant (Chinese chestnut) field adaptability.
This attractant, owing to being have to lure the reactive compound mixture of moth effect to form, can luring a large amount of female moth of dichocrocis punctiferalis of collection and a small amount of male moth, not only can be applied to Li Yuan dichocrocis punctiferalis population occurrence dynamics Supervise prevention and cure, and economical and effective, non-environmental-pollution.
Embodiment two
The present embodiment provide a kind of lure collection dichocrocis punctiferalis lure core, this lures core to be with green rubber plug for carrier, and in described carrier, injection adds the attractant of embodiment one, and described attractant is infiltrated enter described carrier inside to be formed lure core.Eachly the preferred using dosage of attractant in core is lured to be 120.0 μ g.
Above-mentionedly lure in core, with green rubber plug for carrier, in described carrier, injection adds the attractant of described claim 1 or 2, comprising:
Described carrier soaked in absolute ethyl alcohol is cleaned for several times, dries in the air under room temperature and put that ethanol volatilized is clean;
After the attractant n-hexane of described embodiment one is diluted, be injected in the groove of described carrier by micro syringe by the dosage level of setting, add 10.0 μ L benzinums afterwards and make described attractant complete wetting enter described carrier inside.
Below in conjunction with concrete preparation and use procedure, attractant of the present invention is described in further detail.
First, by active volatile compound australene, amphene, sabinene and nopinene in mass ratio for 28:9:37:26 mixture becomes attractant;
Next utilizes above-mentioned attractant to make and lures core:
Green rubber plug is adopted to be that carrier making lures core, configure carrier rubber stopper soaked in absolute ethyl alcohol and clean for several times, drying in the air under room temperature to put makes ethanol volatilize totally, then according to set dosage level (120.0 μ g/ lure core), by attractant with after n-hexane dilution, get 10.0 μ L by micro syringe to be injected in the groove of rubber stopper, and immediately add 10.0 μ L benzinums enter carrier inside to impel volatile scent compounds complete wetting.
The above-mentioned obtained core that lures is utilized to make trapper:
Testing trapper used is basin type trapper, get the little basin of green plastic (internal diameter 25.0cm, dark 10.0cm), inject basin by with clear water and formulated 0.5% detergent solution of washing powder, lure core thin wire to be fixed on above the basin center of circle to be about 1.0cm place apart from the water surface by what prepare.
Above-mentioned obtained attractant is used to carry out field trapping test:
Field trapping test carries out on September 16,31 days to 2013 July in 2013 at Chestnut Germplasm station, Huairou District, Beijing City.Test site is positioned at Huairou District, Beijing City and obtains arm town, and Li Yuan area is about 100hm
2, Main Cultivars is that swallow is red, bosom is yellow and bosom nine, and nearly 30 years of the average age of tree of chestnut, average plant height is about 6.0m.According to the points distributing method placing a trapper at interval of 15.0m, be suspended on apart from the branch of ground 1.2 ~ 1.5m, the trapper at difference position place is for lay at random, and every 7d rearranges a trapper and hangs orientation to the impact of trap effect to reduce.From hanging up second day that lures core, every day, early 7:00 regular check lured moth situation, and record induced moth number also pulls dead moth out.Every 5d changes a detergent solution, and every 25d changes and once lures core, Continuous Observation 46d.With commercial dichocrocis punctiferalis sex pheromone lure core for contrast, often 3 repetitions are established in process.
Result and analysis:
The field trapping test of botanical attractant:
From field gathering effect, attractant of the present invention and commercial dichocrocis punctiferalis the synthetic sex pheromone differ greatly, 3 that carry the attractant of the embodiment of the present invention lure core (i.e. 3 repetitions) 46d accumulation induced moth number to reach 116, and the accumulation induced moth number of 3 commercial sex pheromone lure core is 31, the field accumulation induced moth number of two kinds of attractants forms significant difference.
From the result utilizing botanical attractant to monitor Li Yuan dichocrocis punctiferalis population occurrence dynamics, fruit display is assembled: dichocrocis punctiferalis adult is increased suddenly at the generating capacity on August 24 with the luring of attractant of the embodiment of the present invention, 3 lure core day total induced moth number to be 10,1 larger peak of occurrence was there is to August 25, induced moth number reaches 45, August 26 generating capacity anticlimax, induced moth number is 15; Afterwards, there is 1 little peak in September 10,3 lure core day total induced moth number to be 12, and other times day total induced moth number all lower (0 ~ 4), the mid-September continuous a few days does not trap dichocrocis punctiferalis adult (see Fig. 1,2, what the Lpv in Fig. 1 and Fig. 2 was carrying attractant of the present invention lures core, and Lck is and commercially lures core).Commercial dichocrocis punctiferalis property lure core lure assemble fruit except induced moth number attractant more of the present invention less except, illustrate in Li Yuan dichocrocis punctiferalis population occurrence dynamics with the embodiment of the present invention lure core lure assemble fruit consistent, illustrate that dichocrocis punctiferalis adult has an occurrence in peak period in Beijing area about mid or late August, also prove that the attractant formula of the embodiment of the present invention can be used for monitoring population occurrence dynamics and the pests occurrence rule of Li Yuan dichocrocis punctiferalis simultaneously.
In sum, attractant induced moth number of the present invention is large, environmentally safe, while being applied to the monitoring of Li Yuan dichocrocis punctiferalis population occurrence dynamics, also dichocrocis punctiferalis adult controlling can be directly used in, undesirable and cause the problems such as environmental pollution to solve larval phase effect of chemical control.In order to improve control efficiency, on average every mu can use trapper 2 ~ 3, and notes pulling the moth luring and collect and arrive in time out.
Up to the present, in the world only about the report of dichocrocis punctiferalis the synthetic sex pheromone formula, the report utilizing host plant volatile as attractant is not yet had.Applicant finds that attractant of the present invention is with the volatile active compound australene of dichocrocis punctiferalis host plant (Chinese chestnut), amphene, sabinene and nopinene, according to 28:9:37:26 mass ratio mixture, when luring the dosage of core to trap with 120.0 μ g/, the a large amount of female moth of dichocrocis punctiferalis of collection and a small amount of male moth can be lured, not only can be applied to Li Yuan dichocrocis punctiferalis population occurrence dynamics Supervise prevention and cure, and economical and effective, non-environmental-pollution.The present invention utilizes head space dynamic gas sampling method to extract in China first and identifies the volatile scent compounds of dichocrocis punctiferalis host plant-Chinese chestnut band fruit branch bar, and choose the volatile active compound preparation attractant wherein dichocrocis punctiferalis adult to attracting action, this attractant not only effectively can lure a large amount of female moth of dichocrocis punctiferalis of collection and a small amount of male moth, and has important function and meaning for Li Yuan dichocrocis punctiferalis population dynamic Supervise prevention and cure.
The above; be only the present invention's preferably embodiment, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (4)
1. lure an attractant for collection dichocrocis punctiferalis, it is characterized in that, this attractant is formed by compound australene, amphene, sabinene and nopinene mixture;
Described each compound amount is in mass ratio: australene: amphene: sabinene: nopinene=28:9:37:26.
2. attractant as claimed in claim 1, it is characterized in that, described compound australene, amphene, sabinene and nopinene are the volatile active compound extracted from dichocrocis punctiferalis main host plants plant Chinese chestnut.
3. what lure collection dichocrocis punctiferalis lures a core, and it is characterized in that, this lures core to be with green rubber plug for carrier, and in described carrier, injection adds the attractant of described claim 1 or 2, and described attractant is infiltrated enter described carrier inside to be formed to lure core.
4. lure core as claimed in claim 3, it is characterized in that, described with green rubber plug for carrier, in described carrier, injection adds the attractant of described claim 1 or 2, comprising:
Described carrier soaked in absolute ethyl alcohol is cleaned for several times, dries in the air under room temperature and put that ethanol volatilized is clean;
After the attractant n-hexane dilution described in described claim 1 or 2, be injected in the groove of described carrier by micro syringe by the dosage level of setting, add 10.0 μ L benzinums afterwards and make described attractant complete wetting enter described carrier inside;
The dosage of described setting is each attractant luring core to add 120.0 μ g.
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| CN201310594690.3A CN103583517B (en) | 2013-11-21 | 2013-11-21 | Attractant for luring and gathering dichocrocis punctiferalis and bait |
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| CN201310594690.3A CN103583517B (en) | 2013-11-21 | 2013-11-21 | Attractant for luring and gathering dichocrocis punctiferalis and bait |
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| CN103583517B true CN103583517B (en) | 2015-05-13 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304694B (en) * | 2014-08-27 | 2017-06-06 | 河南科技大学 | Dichocrocis punctiferalis man-made feeds, preparation method and continuously rearing method |
| CN105145561B (en) * | 2015-04-07 | 2020-04-14 | 华南农业大学 | Plant-derived attractant for Chinaberry moth borer |
| CN105410072A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Method for preventing and treating yellow peach dichocrocis punctiferalis |
| CN105410052A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Dichocrocis punctiferalis attractive toxicant |
| CN106857512A (en) * | 2017-02-23 | 2017-06-20 | 童玉扁 | A kind of blastophagus piniperda attractant |
| CN107517964A (en) * | 2017-09-05 | 2017-12-29 | 浙江省农业科学院 | A kind of active compound based on α firpenes and n-octyl alcohol, striped rice borer attractant and application |
| CN107860905B (en) * | 2017-10-24 | 2019-09-13 | 北京农学院 | There is the measuring method and application of the volatile active compound of attracting action to dichocrocis punctiferalis |
| CN108040996B (en) * | 2017-12-11 | 2021-03-02 | 山东省果树研究所 | Adult dichocrocis punctiferalis luring, preventing and controlling device and application |
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| JPS5651405A (en) * | 1979-10-05 | 1981-05-09 | Norin Suisansyo Ringyo Shikenjo | Attractant for pine weevil |
| CN102550554A (en) * | 2011-12-31 | 2012-07-11 | 江苏出入境检验检疫局动植物与食品检测中心 | Broad-spectrum attractant for needle leave wood pests |
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|---|---|---|---|---|
| JPS5651405A (en) * | 1979-10-05 | 1981-05-09 | Norin Suisansyo Ringyo Shikenjo | Attractant for pine weevil |
| CN102550554A (en) * | 2011-12-31 | 2012-07-11 | 江苏出入境检验检疫局动植物与食品检测中心 | Broad-spectrum attractant for needle leave wood pests |
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