CN103548855A - Zhongshengmycin-containing bactericidal composition and application thereof - Google Patents
Zhongshengmycin-containing bactericidal composition and application thereof Download PDFInfo
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Abstract
The invention discloses a zhongshengmycin-containing bactericidal composition of which the effective components are zhongshengmycin and symplectic bacteria amine, wherein the weight ratio of zhongshengmycin to symplectic bacteria amine is 1: (1-40); zhongshengmycin-containing bactericidal composition also comprises auxiliary materials which are allowed to be used and acceptable in pesticide science; the dosage form of the zhongshengmycin-containing bactericidal composition can be any of wettable powder, suspending agent, missible oil, emulsion in water, water dispersible granules or microemulsion. According to the zhongshengmycin-containing bactericidal composition, the action mechanisms of the zhongshengmycin and the symplectic bacteria amine in the effective components are completely different from each other, and the hongshengmycin and the symplectic bacteria amine can realize multi-point action for pathogenic bacteria after being compounded with each other, so that the bactericidal spectrum is expanded, the prevention effect is improved, and the drug resistance is delayed. The zhongshengmycin-containing bactericidal composition can be used for controlling multiple diseases of fruit trees and vegetables, and is especially suitable for soil-borne bacterial diseases such as fruit tree root cancers, ginger bacterial wilt and the like as well as soil-borne fungal diseases such as root rot and the like of crops such as the fruit trees, the vegetables, etc.
Description
Technical field
The present invention relates to a kind of bactericidal composition and application thereof containing Zhongshengmycin, belong to technical field of pesticide.
Background technology
In recent years, variation along with pattern of farming, weather conditions, soil-borne disease of crop occurrence degree and the loss causing increase the weight of year by year, have a strong impact on the yield and quality of crops, to peasant household, cause heavy economic losses, if tree root knot, root rot, bacterial wilt of ginger, and fusarium wilt of various crop, damping off etc.Meanwhile, along with people more and more pay attention to brand promotion and the intensive cropping of agricultural product, some areas are plantation monocrops continuously throughout the year, are also the major reasons that soil-borne disease is difficult to radical cure more.
Grower continues to increase the usage amount of the medicaments such as carbendazim, thiophanate-methyl, metalaxyl, tmtd, and not only control efficiency is undesirable, goes back pollution of ecological environment, increases cost.In addition; in corps diseases; fungal disease and bacterial disease usually mix generation; produce at present upper shortage, to mixing, the medicament that disease all has good preventive effect occurs; and have more problems and shortcomings, as bactericide antibiotics medicament easily develops immunity to drugs, copper agent easily causes the problems such as chemical injury of crops; cause the diseases such as some disease if tree root knot, bacterial wilt of ginger to occur more and more serious, often cause crushing harm.Therefore, in the urgent need to inventing a kind of efficient, low toxic and environment-friendly of both having saved, control plant soil-borne bacterium that again can expanding prevention spectrum, the composition pesticide of fungal disease.
Zhongshengmycin is a kind of novel agricultural antibiotic, and its fungicidal spectrum is wide, and the bacterial disease of crops and fungal disease are all had to good preventive effect, can anti-bacteria bacterium protein synthetic, cause thalline dead; Fungus spore germination is had to strong inhibitory action, cause protoplasm cohesion in hyphal cell, change the form of thread mycelia.
A kind of environment-friendly type amino acids of pungent bacterium amine high molecular polymer bactericide, the mechanism of action is that hydrophilic group partly contains strong electropositive, absorption is each bacterioid, the virus of negative electricity conventionally, thereby the breeding of anti-bacteria, virus, solidify germ protein, cause germ enzyme denaturation, add that the film of polymer formation has stopped up the ion channel of this part microorganism, makes its " death by suffocation " immediately simultaneously.To four subphylum class fungies such as imperfect fungus, basidiomycetes, mastigomycetes, sac fungi and various bacteria, there is fabulous prevention, treat, root out effect, long-term use does not produce resistance.
In the real process of agricultural production, long-term single a kind of medicament of use continuously, pathogen will soon develop immunity to drugs, and causes preventive effect to reduce, and agricultural chemicals usage amount increases, the destruction of aggravation Practice for Pesticide Residue in Agricultural Products and ecotope.By carrying out compositely with the diverse bactericide kind of the mechanism of action, be to delay pathogen to develop immunity to drugs, expand fungicidal spectrum, increase the service life, reduce the effective way of agricultural chemicals usage amount.
In prior art, there is not yet about Zhongshengmycin and pungent bacterium amine is composite and application relevant report.
Summary of the invention
For above-mentioned prior art, for fear of long-continued use single dose, easily make pathogen develop immunity to drugs, cause preventive effect to reduce, agricultural chemicals usage amount increases, the present invention, by composition pesticide being carried out to symphyogenetic quantitative analysis and a large amount of other test, provides a kind of environmental protection type agricultural chemical bactericidal composition that the fungicidal spectrum with synergistic function is wide, cost is low, preventive effect is good that contains Zhongshengmycin, two kinds of active components of pungent bacterium amine.
The present invention is achieved by the following technical solutions:
Containing a bactericidal composition for Zhongshengmycin, active ingredient is Zhongshengmycin and pungent bacterium amine, and the weight ratio of Zhongshengmycin and pungent bacterium amine is 1:1～40, preferred, is 1:1～30.
Described containing in the bactericidal composition of Zhongshengmycin, can contain in Pesticide Science and allow to use and acceptable auxiliary material.
Further, the described bactericidal composition containing Zhongshengmycin, is comprised of the component of following percetage by weight: Zhongshengmycin 1%～80%, and pungent bacterium amine 1%～80%, surplus is in Pesticide Science, to allow to use and acceptable auxiliary material.
In described Pesticide Science, allow to use and acceptable auxiliary material be selected from solvent, dispersant, emulsifier, stabilizing agent, wetting agent, defoamer etc. and other contribute to the stable of active ingredient drug effect and bring into play allow to make used additives.
The described bactericidal composition containing Zhongshengmycin, can be prepared into and be applicable to any one formulation that agricultural production is used by processing method well known in the prior art, preferred dosage form be: wetting powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules, microemulsion.
Bactericidal composition containing Zhongshengmycin of the present invention, for preventing and treating the multiple diseases on fruit tree, vegetables, is particularly useful for the soil such as fruit root knot disease, bacterial wilt of ginger and passes bacterial disease, and the soil-borne fungus venereal disease evils such as root rot of the crop such as fruit tree, vegetables.
Bactericidal composition containing Zhongshengmycin of the present invention, active ingredient Zhongshengmycin, the pungent bacterium amine mechanism of action are completely different, compositely can expand fungicidal spectrum to the effect of pathogen multidigit point, improve preventive effect, delay drug-fast generation.
Bactericidal composition containing Zhongshengmycin of the present invention, can finished product preparation form provide, and also can single dose form provide, and before using, directly mixes, and is then watered to mix to be made into desired concn, and plant root is filled with and executed.During concrete application, also can mix use as insecticide, growth regulator, soil conditioner, weed killer herbicide, nematocide etc. with other medicament.
Bactericidal composition containing Zhongshengmycin of the present invention, has the following advantages: 1, have synergistic function, improve control efficiency; 2, expand fungicidal spectrum, field disease polyhybird occurs, Zhongshengmycin, the mixed double fungus and bacterium disease of controlling of energy of using of pungent bacterium amine; 3, two kinds of active ingredient mechanisms of action are different, are mixed to use to delay the drug-fast generation of pathogen; 4, reduce formulation rate, reduce access times, reduce use cost.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
The composite research of the preventive and therapeutic effect to disease of experiment Zhongshengmycin and pungent bacterium amine (Toxicity Determination)
One, reagent agent: the former medicine of Zhongshengmycin, the former medicine of pungent bacterium amine and the former medicine of kasugarnycin, the former medicine of jinggangmeisu, the former medicine of agricultural streptomycin.Zhongshengmycin, kasugarnycin, jinggangmeisu, the direct water of the former medicine of agricultural streptomycin dissolve the mother liquor that is made into 10000 μ g/mL; By the former medicinal acetone solution of pungent bacterium amine, and be made into the mother liquor of 10000 μ g/mL, the volume fraction with 0.2% adds emulsifier Tween-80, preserves standby in refrigerator at 4 ℃.
Two, for examination pathogen: bacteria pathogeny bacterium: falsesour cherry root carninomatosis bacterium (Agrobacterium tumefaciens (Smith & Town) Conn), ginger plague germ (Ralstonia solanacearum); Fungal pathogens: armillariella root rot of apple bacterium (Fusarium oxysporum).
Three, assay method:
(1) bacterial virulence assay method
It is test material that pathogenetic bacteria toxicity test be take falsesour cherry root carninomatosis bacterium, ginger plague germ, adopts inhibition zone method to carry out toxicity test to Zhongshengmycin, pungent bacterium amine and mixture preparation thereof.
Single dose toxicity test method:
First reagent agent (is comprised to Zhongshengmycin, pungent bacterium amine, kasugarnycin, jinggangmeisu, agricultural streptomycin) be mixed with 2.0, 4.0, 8.0, 16.0, 32.0, 64.0 and 7 concentration gradients such as 128.0mg/kg, the bacteria suspension 2mL(bacteria containing amount of getting diluted for use is about 106CFU/mL) to the diameter culture dish that is 90mm, NA medium 15mL(40～50 ℃ that add sterilizing to dissolve), fully mix, after its cooled and solidified, 1 hole is made a call to respectively at the card punch that is 5mm with diameter 1/4 and 3/4 place on the diameter line of every ware medium, in every hole, add 50 μ L nutrient agar shop fixtures, after it solidifies, in wherein 1 hole of each culture dish, add respectively the above-mentioned preparation medicament of 70 μ L, another 1 hole adds sterile water to compare, 5 repetitions of every concentration for the treatment of, after medium is cultivated to 24～36h at 28 ℃, measure antibacterial circle diameter, the mean value of getting effective 3 repetitions is measurement result, calculate relative inhibition, after inhibiting rate is converted to probability value, by method of least squares, calculate the toxicity regression formula of antibacterial probability value and reagent agent concentration logarithm, obtain each medicament to concentration EC in effective inhibition of bacterial strain
50.Test data is carried out statistical analysis by Microsoft Excel2003, DPS data processing work platform.
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, by the Zhongshengmycin that Gong is mixed, pungent bacterium amine respectively by its EC
50value dosage arranges respectively 5: 1 by weight proportion, 2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings, by different proportionings, being mixed with final mass concentration is 2.0,4.0,8.0,16.0,32.0,64.0 and the pastille NA medium of 128.0mg/kg7 series concentration, determines the inhibiting rate of medicament to pathogen growth.Every processing (the every concentration level of every bacterial strain) repeats 4 times.By the linear regression analysis between pathogen growth inhibition probable value and drug concentration logarithm value, obtain Mixed Pharmacy to concentration EC in effective inhibition of pathogen
50value and CTC value, with the NA medium of adding medicine not in contrast.
(2) fungi toxicity test method
Disease fungus toxicity test be take armillariella root rot of apple bacterium as test material, adopts mycelial growth rate method to carry out toxicity test to Zhongshengmycin, pungent bacterium amine and mixture preparation thereof.
Single dose toxicity test method: by pathogen 26 ℃ of preculture 5d on PDA medium, with the card punch of diameter 7mm, near beating on the same circumference of colony edge, getting bacterium cake, be inoculated into and contain different medicaments and (comprise Zhongshengmycin, pungent bacterium amine, kasugarnycin, jinggangmeisu, agricultural streptomycin) concentration (0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 7 concentration gradients such as 16.0mg/kg) on culture medium flat plate, every ware a slice, be placed in cultivation 4d in 26 ℃ of insulating boxs, by right-angled intersection method, measure the colony growth diameter that each is processed, take clear water as contrast, every concentration is processed and is repeated 4 times.Measure the radially linear amount of growth of bacterium colony, determine the inhibiting rate of medicament to colony growth.By mycelial growth, suppress the linear regression analysis between probable value and drug concentration logarithm value, obtain each medicament to concentration (EC in effective inhibition of bacterial strain
50value).
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, by the Zhongshengmycin that Gong is mixed, pungent bacterium amine respectively by its EC
50value dosage arranges respectively 5: 1 by weight proportion, 2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings, by different proportionings, be mixed with that final mass concentration is 0.25,0.5,1.0,2.0,4.0,8.0, the pastille PDA medium of a 16.0mg/kg7 series concentration, determine the inhibiting rate of medicament to colony growth.Every processing (the every concentration level of every bacterial strain) repeats 4 times.By mycelial growth, suppress the linear regression analysis between probable value and drug concentration logarithm value, obtain Mixed Pharmacy to concentration EC in effective inhibition of bacterial strain
50value and CTC value, with the PDA medium of adding medicine not in contrast.
(3) data statistic analysis
Test data is carried out statistical analysis by Microsoft Excel2003, DPS data processing work platform, calculates the EC of every kind of medicament
50value, 95% confidence limit.Utilize the abundant method of Sun Yun (1960) to calculate the co-toxicity coefficient of mixed agent, according to co-toxicity coefficient size, evaluate the synergistic effect of mixed agent.
According to following formula, calculate each medicament inhibiting rate:
Suppress growth rate (%)=[(contrast antibacterial circle diameter-processings antibacterial circle diameter)/(contrast antibacterial circle diameter-5mm punch diameter)] * 100
Suppress growth rate (%)=[(contrast colony diameter-processing colony diameter)/(contrast colony diameter-bacterium cake diameter)] * 100
Toxicity index TI=(standard medicament EC
50/ reagent agent EC
50) * 100
The actual toxicity index ATI=(standard of mixed agent medicament EC
50/ mixture preparation EC
50) * 100
TI * PB(PA of TI * PA+ single dose B of the theoretical toxicity index TTI=of mixed agent single dose A and the percentage composition that PB is respectively active ingredient in mixed agent)
Theoretical toxicity index TTI * 100 of the actual toxicity index ATI/ mixed agent of co-toxicity coefficient CTC=mixed agent
Synergistic effect judgement: CTC >=120, have synergistic effect; 80 < CTC < 120 are summation action; CTC≤80 are antagonism.
(4) results and analysis: as shown in table 1～table 6.
The toxicity test result of 5 kinds of medicaments of table 1 to falsesour cherry root carninomatosis bacterium
The toxicity test result that table 2 Zhongshengmycin and pungent bacterium amine different proportion are mixed to falsesour cherry root carninomatosis bacterium
From table 1, table 2, Zhongshengmycin, pungent bacterium amine and mixture preparation thereof, falsesour cherry root carninomatosis bacterium is had to good inhibitory action, when wherein the proportioning of Zhongshengmycin and pungent bacterium amine is between 1:1～1:40, to supplying the co-toxicity coefficient of examination pathogen all more than 120, show that these two kinds of bactericide have the synergistic effect between 1:2～1:25 of good synergistic function, especially proportioning under this proportioning more remarkable.
The toxicity test result of 5 kinds of medicaments of table 3 to ginger plague germ
The toxicity test result that table 4 Zhongshengmycin and pungent bacterium amine different proportion are mixed to ginger plague germ
From table 3, table 4, Zhongshengmycin, pungent bacterium amine and mixture preparation thereof, ginger plague germ is had to good inhibitory action, when wherein the proportioning of Zhongshengmycin and pungent bacterium amine is between 1:1～1:40, to supplying the co-toxicity coefficient of examination pathogen all more than 120, show that these two kinds of bactericide have the synergistic effect between 1:5～1:30 of good synergistic function, especially proportioning under this proportioning more remarkable.
The toxicity test result of 5 kinds of medicaments of table 5 to armillariella root rot of apple bacterium
The toxicity test result that table 6 Zhongshengmycin and pungent bacterium amine different proportion are mixed to armillariella root rot of apple bacterium
From table 5, table 6, Zhongshengmycin, pungent bacterium amine and mixture preparation thereof, armillariella root rot of apple bacterium is had to good inhibitory action, when wherein the proportioning of Zhongshengmycin and pungent bacterium amine is between 1:1～1:40, to supplying the co-toxicity coefficient of examination pathogen all more than 120, show that these two kinds of bactericide have the synergistic effect between 1:5～1:20 of good synergistic function, especially proportioning under this proportioning more remarkable.
The pungent bacterium amine of embodiment 1 3% Zhongshengmycin wetting powder
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 2%, Negel formaldehyde condensation products (dispersant) 2%, sodium lignin sulfonate (dispersant) 3%, lauryl sodium sulfate (wetting agent) 3%, White Carbon black (filler) 20%, kaolin (filler) complements to 100%.
Preparation method is: above-mentioned each active component, various auxiliary agent and filler are fully mixed in proportion, and mixture, through ultra-fine pulverizer disintegrating, makes the pungent bacterium amine of 3% Zhongshengmycin wetting powder.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 7.
The field effect of table 7 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 7, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
The pungent bacterium amine of embodiment 2 21% Zhongshengmycin suspending agent
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 20%, Negel formaldehyde condensation products (dispersant) 5%, castor oil polyoxyethylene ether (emulsifier) 3%, fatty alcohol-polyoxyethylene ether (emulsifier) 5%, sodium methylene bis-naphthalene sulfonate (wetting agent) 3%, xanthans (thickener) 2%, glycerine (antifreeze) 4%, water complements to 100%.
Preparation method is: each active component, various auxiliary agent and water are fully mixed in proportion, drop into sand mill, be ground to particle diameter 5 μ m, make the pungent bacterium amine of 21% Zhongshengmycin suspending agent.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 8.
The field effect of table 8 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 8, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
The pungent bacterium amine of embodiment 3 11% Zhongshengmycin missible oil
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 10%, cyclohexane (solvent) 25%, agricultural emulsifier NO．600 (agricultural emulsifier NO．600, for existing conventional products in prior art, can market buy and obtain) (emulsifier) 3%, castor oil polyethylene glycol condensation (emulsifier) 5%, glycerine (antifreeze) 4%, dimethylbenzene complements to 100%.
Preparation method is: each active component, various auxiliary agent and water are fully mixed in proportion, add in mixing kettle, the pungent bacterium amine of 11% Zhongshengmycin missible oil stirs to obtain.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 9.
The field effect of table 9 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 9, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
The pungent bacterium amine of embodiment 4 16% Zhongshengmycin aqueous emulsion
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 15%, cyclohexane (solvent) 20%, agricultural emulsifier NO．600 (emulsifier) 3%, castor oil polyethylene glycol condensation (emulsifier) 5%, Negel formaldehyde condensation products (dispersant) 5%, water complements to 100%.
Preparation method is: each active component, various auxiliary agent are fully mixed in proportion, make to be dissolved into even oil phase, under high-speed stirred, oil phase is mixed with water, obtains the pungent bacterium amine of 16% Zhongshengmycin aqueous emulsion.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 10.
The field effect of table 10 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 10, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
The pungent bacterium amine of embodiment 5 6% Zhongshengmycin water dispersible granules
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 5%, Negel formaldehyde condensation products (dispersant) 5%, sodium lignin sulfonate (dispersant) 5%, sodium methylene bis-naphthalene sulfonate (wetting agent) 2%, ammonium sulfate (disintegrant) 3%, White Carbon black (filler) 30%, calcium carbonate (filler) complements to 100%.
Preparation method is: each active component, Negel formaldehyde condensation products, wetting agent, disintegrant, filler are fully mixed in proportion, through airslide disintegrating mill, be crushed to particle diameter 5 μ m, obtain female powder, female powder is fully mixed to rear granulation, dries, sieves with sodium lignin sulfonate, thereby make the pungent bacterium amine of 6% Zhongshengmycin water-dispersible granules.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 11.
The field effect of table 11 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 11, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
The pungent bacterium amine of embodiment 6:26% Zhongshengmycin microemulsion
Component (each components based on weight percentage) composed as follows:
Zhongshengmycin 1%, pungent bacterium amine 25%, cyclohexane (solvent) 15%, agricultural emulsifier NO．600 (emulsifier) 3%, castor oil polyethylene glycol condensation (emulsifier) 5%, isopropyl alcohol (solvent) 15%, water complements to 100%.
Preparation method is: each active component, various auxiliary agent are fully mixed in proportion, make to be dissolved into even oil phase, under high-speed stirred, oil phase is mixed with water, obtains the pungent bacterium amine of 26% Zhongshengmycin microemulsion.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws a furrow irrigation root, and every 15 days once, connects and execute 3 times.Control efficiency sees the following form 12.
The field effect of table 12 Zhongshengmycin preparation control different from pungent bacterium amine falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple
By table 12, can find out, after Zhongshengmycin and pungent bacterium amine are mixed synergistic effect obvious, to the control efficiency of three kinds of diseases, significantly better than single dose, and effective ingredient consumption obviously reduces.
Claims (10)
1. containing a bactericidal composition for Zhongshengmycin, it is characterized in that: active ingredient is Zhongshengmycin and pungent bacterium amine, and the weight ratio of Zhongshengmycin and pungent bacterium amine is 1:1～40.
2. the bactericidal composition containing Zhongshengmycin according to claim 1, it is characterized in that: the described bactericidal composition containing Zhongshengmycin is comprised of the component of following percetage by weight: Zhongshengmycin 1%～80%, pungent bacterium amine 1%～80%, surplus is in Pesticide Science, to allow to use and acceptable auxiliary material.
3. the bactericidal composition containing Zhongshengmycin according to claim 1, is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is wetting powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules or microemulsion.
4. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is wetting powder, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 2%, Negel formaldehyde condensation products 2%, sodium lignin sulfonate 3%, lauryl sodium sulfate 3%, White Carbon black 20%, surplus is kaolin.
5. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is suspending agent, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 20%, Negel formaldehyde condensation products 5%, castor oil polyoxyethylene ether 3%, fatty alcohol-polyoxyethylene ether 5%, sodium methylene bis-naphthalene sulfonate 3%, xanthans 2%, glycerine 4%, surplus is water.
6. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is missible oil, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 10%, cyclohexane 25%, agricultural emulsifier NO．600 3%, castor oil polyethylene glycol condensation 5%, glycerine 4%, surplus is dimethylbenzene.
7. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is aqueous emulsion, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 15%, cyclohexane 20%, agricultural emulsifier NO．600 3%, castor oil polyethylene glycol condensation 5%, Negel formaldehyde condensation products 5%, surplus is water.
8. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: the formulation of the described bactericidal composition containing Zhongshengmycin is water dispersible granules or microemulsion;
When formulation is water dispersible granules, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 5%, Negel formaldehyde condensation products 5%, sodium lignin sulfonate 5%, sodium methylene bis-naphthalene sulfonate 2%, ammonium sulfate 3%, White Carbon black 30%, surplus is calcium carbonate;
When formulation is microemulsion, by the component of following percetage by weight, formed: Zhongshengmycin 1%, pungent bacterium amine 25%, cyclohexane 15%, agricultural emulsifier NO．600 3%, castor oil polyethylene glycol condensation 5%, isopropyl alcohol 15%, surplus is water.
9. the application in the disease of the bactericidal composition containing Zhongshengmycin described in claim 1～8 on control fruit tree, vegetables.
10. application according to claim 9, is characterized in that: described disease is fruit root knot disease, root rot, bacterial wilt of ginger.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103901128A (en) * | 2014-03-27 | 2014-07-02 | 深圳诺普信农化股份有限公司 | Analysis method of Zhongshengmycin content |
| CN103960286A (en) * | 2014-05-06 | 2014-08-06 | 江苏东宝农药化工有限公司 | Synergistic fungicide composition containing bacillus licheniformis and preparation method thereof |
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| CN1046440A (en) * | 1989-10-04 | 1990-10-31 | 山东省人才开发服务中心化工技术开发公司 | The prescription of compound disinfectant-octicin solution, range of application and using method thereof |
| CN102388887A (en) * | 2011-08-02 | 2012-03-28 | 南宁市德丰富化工有限责任公司 | Sterilization composition containing zhongshengmycin |
-
2013
- 2013-11-15 CN CN201310574370.1A patent/CN103548855B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1046440A (en) * | 1989-10-04 | 1990-10-31 | 山东省人才开发服务中心化工技术开发公司 | The prescription of compound disinfectant-octicin solution, range of application and using method thereof |
| CN102388887A (en) * | 2011-08-02 | 2012-03-28 | 南宁市德丰富化工有限责任公司 | Sterilization composition containing zhongshengmycin |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901128A (en) * | 2014-03-27 | 2014-07-02 | 深圳诺普信农化股份有限公司 | Analysis method of Zhongshengmycin content |
| CN103901128B (en) * | 2014-03-27 | 2016-04-06 | 深圳诺普信农化股份有限公司 | A kind of analytical approach of Zhongshengmycin content |
| CN103960286A (en) * | 2014-05-06 | 2014-08-06 | 江苏东宝农药化工有限公司 | Synergistic fungicide composition containing bacillus licheniformis and preparation method thereof |
| CN103960286B (en) * | 2014-05-06 | 2018-07-03 | 江苏东宝农化股份有限公司 | A kind of synergy fungicide composite containing bacillus licheniformis and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN103548855B (en) | 2014-08-20 |
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