CN103548561A - Novel cordyceps militaris harvest efficiency improving method - Google Patents
Novel cordyceps militaris harvest efficiency improving method Download PDFInfo
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- CN103548561A CN103548561A CN201310492706.XA CN201310492706A CN103548561A CN 103548561 A CN103548561 A CN 103548561A CN 201310492706 A CN201310492706 A CN 201310492706A CN 103548561 A CN103548561 A CN 103548561A
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Abstract
The invention relates to a novel cordyceps militaris harvest efficiency improving method. According to the novel cordyceps militaris harvest efficiency improving method, culture medium covering nets can prevent rice culture medium particles from sticking to fresh sporocarp, so that harvesting efficiency can be achieved, the harvesting cost can be saved and the quality of harvested sporocarp products can be improved.
Description
Technical field
The present invention relates to cordyceps cultivation technology field, be specifically related to a kind of Cordyceps militaris scale production process and method.
Background technology
Cordyceps militaris (Cordyceps militaris (L.Fr) Link), belongs to Ascomycota (Ascomycota), gang pyrenomycetes (Pyrenomycetes), Sphaerials (Sphaeriales), Clavicipitaceae (Clavicipitaceae), Cordyceps (Cordyceps (Fr.) Link) fungi.Cordyceps militaris is the type sepecies of Cordyceps sinensis fungus, on the larva and pupa of the multiple squama wing of natural world multiparasitism day insect.
In recent years, research shows, tame Cordyceps militaris chemical composition and pharmacological action are similar to Cordyceps sinensis.Therefore, the Application and Development of Cordyceps militaris has great potential market.Tissue Culture of Cordyceps militaris is to solve the not enough good approach in wild Chinese caterpillar fungus source.
Current scale Cordyceps militaris all adopts semi-artificial synthetic medium to carry out large-scale planting in producing, and main cause is that wide material sources and the easy preservation of raw material (rice, millet, Chinese sorghum, corn, milk powder, soya bean etc.) is not subject to the restriction in season.
When fruiting bodies of cordyceps militaris is gathered in the crops, usually there is rice on medium to stick in fruit body dry together, in dry products process, the large grain of rice adhering to be taken out, need to consume very large artificial.
Summary of the invention
The object of the invention is to improve the Cordyceps militaris efficiency of gathering, reduce the cost of gathering, improve gather after fruit body product quality.In the process that the present invention can avoid gathering, rice medium particle sticks on new fresh sporophore.
For achieving the above object, the time that the present invention selects the kind of suitable medium nerve of a covering and selects suitable nerve of a covering to cover.Particularly, comprise following content:
1, the kind of medium nerve of a covering:
Kind one: adopt spinning gauze, porosity is that 1.5-5mm all can.Be cut into the shape onesize with cultivating in box rice cake, then standby.
Kind two: the plastic wire that adopts the tasteless high-molecular organic material of fire-resistant nontoxic to make, require softness, can be close to a meter cake surface, porosity is that 1.5-5mm all can.Be cut into the shape onesize with cultivating in box rice cake, then standby.
Kind three: adopt stainless (steel) wire, porosity is that 1.5-5mm all can.Be cut into the shape onesize with cultivating in box rice cake, then standby.
Kind four: adopt nylon wire, porosity is that 1.5-5mm all can.Be cut into the shape onesize with cultivating in box rice cake, then standby.
2, the time that nerve of a covering covers:
Cover time one: after installing medium, nerve of a covering is covered to the surface of rice, nutrient mix, go together sterilizing.
Cover time two: by the sterilizing of nerve of a covering mounted box, then cover before culture medium inoculated on meter cake, then inoculation.
Cover time three: by the sterilizing of nerve of a covering mounted box, then, after dark cultivation finishes, behind mycelia uniform fold rice cake surface, nerve of a covering is covered to upper applying light afterwards.
3, Cordyceps militaris culture technology:
All the other are all identical with conventional cordyceps militaris plantation mode.
The inventive method tool has the following advantages:
Experiment results proved of the present invention: avoided rice medium particle in recovery process to stick on new fresh sporophore after adding nerve of a covering, realized and improved the efficiency of gathering, reduced the cost of gathering, improved the object of fruit body product quality after gathering.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
One, experimental period: in April, 2012:
Two, experiment material and design:
(1), bacterial classification: cordyceps militaris link bacterial strain, purchased from Microbe Inst., Chinese Academy of Sciences, is numbered PRB.1235.
(2), bacterial classification solid culture medium: PDA inclined-plane.
(3), liquid shaking bottle medium: peeled potatoes 250 grams of (liquor), 8 grams of glucose, 2.5 grams of peptones, 0.6 gram of potassium dihydrogen phosphate, 1.2 grams, magnesium sulfate, 1000 milliliters, water, pH nature.
(4), fruit body is produced nutrient solution: soya bean 2.2%, glucose 1.5%, milk powder 4.5%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.06%, Cobastab
1the ratio of 15mg ∕ 1000ml is made into nutrient solution, adjusts pH 5.6～7.2.
(5), conventional cultivation culture vessel: high 12cm, diameter 8cm.30 grams, dress rice, nutrient solution 45ml.
(6), rice cake covers and processes: conventional rice cake surface is not covered as processes A.Rice cake surface adopts the nylon wire in 3mm aperture to be covered as treatments B after rice mixes with nutrient solution.The selection of time that nerve of a covering covers, after installing medium, covers nerve of a covering on the surface of rice, nutrient mix, goes together sterilizing.
Three, experimental technique:
(1), the sterilizing of medium:
Conventional sterilizing in 121 ℃, 20 minutes.
(2), inoculating process:
Inoculation head is made to seedpod of the lotus shape and carry out fountain inoculation.
(3), dark cultivation stage:
Postvaccinal medium container is directly put into dark culturing room and cultivate, keep 18-22 ℃ of temperature to carry out lucifuge cultivation, after 6 days, mycelia covers behind whole surface, and dark cultivation finished.
(4), the illumination cultivation stage:
Culturing room's temperature should be (20～23) ℃, in fruit body late stage of culture, strengthens gradually circulation and the exchange of room air, if culturing room's cultivation stage is found to pollute, should remove immediately.When being 5-8cm, fruit body height gathers in time.
(5), gather: after gathering, the average dry weight of fruit body of the present invention reaches 2.76 grams/bottle.After gathering, the average dry weight of conventional method fruit body reaches 2.77 grams/bottle.Output there almost is not difference.But it is rapid that fruit body of the present invention is adopted rear convenience, is not stained with the grain of rice, sorting is convenient.
Conclusion: simple to operate, cost is low, effective.Workman's efficiency of gathering is high, has saved production cost.The not sticky rice of fruit body, has improved product quality.
Embodiment 2
Basic operation is with embodiment 1, and difference is: 1, nerve of a covering is selected the spinning gauze in 4mm aperture; 2, the selection of time that nerve of a covering covers, by the sterilizing of nerve of a covering mounted box, then covers on meter cake before culture medium inoculated, then inoculation.
After gathering, the average dry weight of fruit body of the present invention reaches 2.77 grams/bottle.After gathering, the average dry weight of conventional method fruit body reaches 2.76 grams/bottle.Output there almost is not difference.But it is rapid that fruit body of the present invention is adopted rear convenience, is not stained with the grain of rice, sorting is convenient.
Embodiment 3
Basic operation is with embodiment 1, and difference is: 1, nerve of a covering is selected the plastic wire that the tasteless high-molecular organic material of fire-resistant nontoxic in 2mm aperture is made; 2, the selection of time that nerve of a covering covers, by the sterilizing of nerve of a covering mounted box, then covers on meter cake before culture medium inoculated, then inoculation.
After gathering, the average dry weight of fruit body of the present invention reaches 2.75 grams/bottle.After gathering, the average dry weight of conventional method fruit body reaches 2.76 grams/bottle.Output there almost is not difference.But it is rapid that fruit body of the present invention is adopted rear convenience, is not stained with the grain of rice, sorting is convenient.
Embodiment 4
Basic operation is with embodiment 1, and difference is: 1, nerve of a covering is selected the stainless (steel) wire in 4mm aperture; 2, the selection of time that nerve of a covering covers is by the sterilizing of nerve of a covering mounted box, then after dark cultivation finishes, behind mycelia uniform fold rice cake surface, by applying light after nerve of a covering covering above.
After gathering, the average dry weight of fruit body of the present invention reaches 2.75 grams/bottle.After gathering, the average dry weight of conventional method fruit body reaches 2.75 grams/bottle.Output there almost is not difference.But it is rapid that fruit body of the present invention is adopted rear convenience, is not stained with the grain of rice, sorting is convenient.
Although the present invention is described in detail above to have used general explanation and specific embodiment, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. improve the gather method of efficiency of Cordyceps militaris, it is characterized in that, in incubation, adopt medium nerve of a covering.
2. method claimed in claim 1, is characterized in that, medium nerve of a covering used is selected from following group: gauze, plastic wire, wire netting or nylon wire.
3. method claimed in claim 2, is characterized in that, medium nerve of a covering used is selected from following group: plastic wire, stainless (steel) wire or nylon wire that spinning gauze, the tasteless high-molecular organic material of fire-resistant nontoxic are made.
4. the method described in claim 1-3 any one, is characterized in that, the porosity of described nerve of a covering is 1.5-5mm.
5. the method described in claim 1-4 any one, is characterized in that, the selection of time that its nerve of a covering covers, after installing medium, covers nerve of a covering on the surface of rice, nutrient mix, goes together sterilizing.
6. the method described in claim 1-4 any one, is characterized in that, the selection of time that its nerve of a covering covers, by the sterilizing of nerve of a covering mounted box, then covers on meter cake before culture medium inoculated, then inoculation.
7. the method described in claim 1-4 any one, is characterized in that, the selection of time that its nerve of a covering covers is by the sterilizing of nerve of a covering mounted box, then after dark cultivation finishes, behind mycelia uniform fold rice cake surface, by applying light after nerve of a covering covering above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310492706.XA CN103548561A (en) | 2013-10-18 | 2013-10-18 | Novel cordyceps militaris harvest efficiency improving method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310492706.XA CN103548561A (en) | 2013-10-18 | 2013-10-18 | Novel cordyceps militaris harvest efficiency improving method |
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| CN103548561A true CN103548561A (en) | 2014-02-05 |
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| CN201310492706.XA Pending CN103548561A (en) | 2013-10-18 | 2013-10-18 | Novel cordyceps militaris harvest efficiency improving method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297997A (en) * | 1999-12-02 | 2001-06-06 | 上海国宝企业发展中心 | Culture process of Nortern cordyceps sporophore |
| JP2007215417A (en) * | 2006-02-14 | 2007-08-30 | Karasawa Sangyo:Kk | Cap for mushroom cultivation bottle |
| CN102017865A (en) * | 2009-09-23 | 2011-04-20 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN201830686U (en) * | 2010-08-25 | 2011-05-18 | 福建嘉田农业开发有限公司 | Edible fungus cultivation container cover |
| CN201839643U (en) * | 2010-08-25 | 2011-05-25 | 福建嘉田农业开发有限公司 | Edible fungus cultivation device with detachable separation net |
| CN201846639U (en) * | 2010-08-25 | 2011-06-01 | 福建嘉田农业开发有限公司 | Edible mushroom bowl-shaped cultivating device |
| CN102805004A (en) * | 2012-04-09 | 2012-12-05 | 上海丰科生物科技股份有限公司 | Culture method for bottle culture edible fungus |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN203251678U (en) * | 2013-04-25 | 2013-10-30 | 天津市盘宇农业技术开发有限公司 | Device for culturing edible fungi |
-
2013
- 2013-10-18 CN CN201310492706.XA patent/CN103548561A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297997A (en) * | 1999-12-02 | 2001-06-06 | 上海国宝企业发展中心 | Culture process of Nortern cordyceps sporophore |
| JP2007215417A (en) * | 2006-02-14 | 2007-08-30 | Karasawa Sangyo:Kk | Cap for mushroom cultivation bottle |
| CN102017865A (en) * | 2009-09-23 | 2011-04-20 | 北京农业生物技术研究中心 | Two-stage large-scale cordyceps militaris culture method |
| CN201830686U (en) * | 2010-08-25 | 2011-05-18 | 福建嘉田农业开发有限公司 | Edible fungus cultivation container cover |
| CN201839643U (en) * | 2010-08-25 | 2011-05-25 | 福建嘉田农业开发有限公司 | Edible fungus cultivation device with detachable separation net |
| CN201846639U (en) * | 2010-08-25 | 2011-06-01 | 福建嘉田农业开发有限公司 | Edible mushroom bowl-shaped cultivating device |
| CN102805004A (en) * | 2012-04-09 | 2012-12-05 | 上海丰科生物科技股份有限公司 | Culture method for bottle culture edible fungus |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN203251678U (en) * | 2013-04-25 | 2013-10-30 | 天津市盘宇农业技术开发有限公司 | Device for culturing edible fungi |
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