CN103529543A - Automatic microscope focusing method - Google Patents
Automatic microscope focusing method Download PDFInfo
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- CN103529543A CN103529543A CN201310483352.2A CN201310483352A CN103529543A CN 103529543 A CN103529543 A CN 103529543A CN 201310483352 A CN201310483352 A CN 201310483352A CN 103529543 A CN103529543 A CN 103529543A
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Abstract
The invention provides an automatic microscope focusing method, comprising the steps of utilizing a liquid lens of which the focal length is capable of being controlled by voltage in a microscope optical path to be used as a zooming element, capturing a video stream while performing continuous stepping changing on the driving voltage of the liquid lens, performing focusing evaluation on each captured image, utilizing a focal point searching method to find the liquid lens voltage, positioned at the optimal focusing state, of the microscopic image, and then driving the liquid lens voltage to such a position to finish the automatic focusing. The automatic microscope focusing method fully utilizes the advantages of fast responding speed and small delaying of the liquid lens and solves the problems of slow and inaccurate focusing during the automatic focusing process by using a traditional mobile lifting table.
Description
Technical field
The present invention relates to the micro-vision field in photoelectric detecting technology, and relate more specifically to a kind of method for micro-vision automatic focusing.
Background technology
Autofocus system is the critical component that automatic video frequency microscopic examination, measurement and depth information are rebuild, the research of relevant microscope autofocus system is the focus of microscopic study always, especially the automatic video frequency microscope to large-numerical aperture, high magnification and the little depth of field.
The autofocus system proposing at present mainly contains active autofocus system and the passive auto focus system based on image quality evaluation based on the auxiliary measuring equipment in outside.The optical imaging system that active autofocus system based on the auxiliary measuring equipment in outside is measured according to external measurement devices be imaged the distance that target range regulates the two and realize automatic focusing, but this method Installation and Debugging are complicated, and system architecture is also more complicated, therefore less use.What application was more at present is the passive auto focus system based on image quality evaluation, by the quality assessment of gathered image being searched for to focal position with auto focus evaluation function.Passive auto focus system realizes simple, easy to use, current passive auto focus system mainly adopts motor drive platform movement and utilize motorized zoom lens, and utilize to focus on to evaluate motion is fed back, make observed object be adjusted to microscope working distance place, the method is subject to the restriction of motor precision, vibration, response speed and lag error in motor movement process, causes the restriction of focusing accuracy.
Liquid lens is a kind of novel optical lens based on electrowetting principle, the voltage being added in its two end electrodes by change can change rapidly its focal length accurately, therefore can utilize it to be placed in micro-light path and replace motorized zoom lens, because liquid lens response is fast and stable, can improve speed and the precision of autofocus system.
Summary of the invention
Technology of the present invention is dealt with problems: utilize focus variable liquid lens to realize the automatic focusing of microscopic system, guarantee that micro imaging system can get rapidly HD image accurately.
Technical scheme of the present invention is: the device of realizing the inventive method consists of optical microscope, camera, liquid lens and drive part thereof and automatic focusing system software, liquid lens is fixed on the rear end face of microcobjective by the adaptor specially designing, automatic focusing system software can be realized the Comprehensive Control to liquid lens, electric precise lifting table and camera, can realize a key automatic focusing function simultaneously.Automatic focusing software has been realized synchronous that liquid lens driving voltage step change and Video stream sapture, figure image focu evaluate, by maximum value search algorithm, obtain the liquid lens driving voltage when figure image focu evaluation of estimate is maximum, liquid lens driving voltage is adjusted to this value, and automatic focus completes.
Accompanying drawing explanation
Embodiments of the present invention is described in detail in conjunction with the drawings, and above and other objects of the present invention, feature, advantage will become apparent, wherein:
Fig. 1 is the auto focusing method process flow diagram according to the embodiment of the present invention.
Fig. 2 is the microscopic system index path according to the embodiment of the present invention.
Fig. 3 is the liquid lens converting interface according to the embodiment of the present invention.
Fig. 4 is the liquid lens theory diagram according to the embodiment of the present invention.
Embodiment
Describe according to embodiments of the invention below with reference to accompanying drawings.In the accompanying drawings, identical reference number represents identical element from start to finish.
With reference to accompanying drawing, the microscopy imaging system light path according to the embodiment of the present invention is described.
Fig. 1 shows the auto focusing method process flow diagram of the embodiment of the present invention.In auto-focus process, utilize liquid lens as zoom element, utilize to focus on and evaluate operator Real-Time Evaluation micro-image focus state, utilize focus search algorithm to carry out servocontrol to liquid lens driving voltage, until that micro-image focuses on evaluation of estimate is the highest, micro-image has focused on while reaching the most clear state.
Fig. 2 shows the microscopic system index path according to the embodiment of the present invention.As shown in Figure 2, microscopic system light path 20 comprises testee 21, object lens 22, liquid lens 23, adaptive mirror 24 and detector C CD25.Liquid lens 23 is positioned between object lens 22 and adaptive mirror 24.
Fig. 3 shows the liquid lens converting interface 30 according to the embodiment of the present invention.As shown in Figure 3, adapter consists of two parts 31,32,31 lower thread and 32 top screw threads can couple together, and liquid lens is fixing extremely in the inner, 31 tops can be connected with switching mirror by screw thread, 32 bottoms can be connected with object lens by screw thread, and the groove of 32 side openings can be placed the drive wire of liquid lens simultaneously.
Secondly, brief description principle of the present invention.
First briefly introduce the principle and feature of liquid lens.Liquid zoom lens is a kind of novel optical lens based on electrowetting principle.Fig. 4 shows the fundamental diagram of liquid lens, and liquid lens comprises two kinds of liquid, and a kind of is conducting liquid, and another kind is iknsulating liquid, and two kinds of liquid does not infiltrate mutually and has certain refringence.Two kinds of liquid is contained in the container that inwall is coated with transparency electrode, and the hydrophobic dielectric layer of surface precipitation one deck of transparency electrode like this, just forms two contact angles and surface of contact between two-layer liquid.When applying after voltage between conducting liquid and inwall transparency electrode, between conducting liquid and electrode, electric field changes, thereby the shape of contact angle and surface of contact is changed, and the focal length of liquid lens also just changes.Liquid zoom lens technology is mature on the whole at present, existing market-oriented product, and domestic have agency, and price is also lower, the about hundreds of of a liquid zoom lens unit.Utilize the varifocal characteristic of liquid lens, added in digital micro-analysis imaging system, when the driving voltage of liquid lens changes, its focal length also changes, according to double lens focal length composite formula, the synthetic focal length of known whole microscopic system changes, therefore the object plane that micro imaging system institute can blur-free imaging is apart from also changing, therefore the driving voltage of liquid lens and add the existence relation one to one of the digital optical micro-system of liquid lens, this specific character of liquid lens makes it as a kind of passive ranging element, become possibility.
Then introduce the autofocus evaluation function of the sharpness of quantitative expedition photographic images in the present invention.Because the image of focusing is with respect to out-of-focus image, in spatial domain, show as and there is edge more clearly, more details, in frequency domain rate, there is more high fdrequency component, therefore the focusing evaluation algorithms of much evaluating in spatial domain and frequency is suggested, in invention, we adopt Tenegrad function (as shown in Equation 1) in spatial domain to judge the focusing out-of-focus appearance of image.
Wherein, G
x(x, y) and G
y(x, y) is original image and Sobel operator S
xand S
yconvolution results.Sobel operator is as follows:
Then introduce the maximum value search algorithm in this automatic focusing software, the present invention adopts climbing method, and its basic step is:
1) initial focusing direction is set, along initial focusing directional trim one step, if focus function value slippage surpasses a thresholding, changes focusing direction.
2), along the focusing of the large step-length of focusing direction, return to the maximum of points recording in focusing process after finding focus function drop point.
3), along little step-length of large step-length focusing directional trim, if focus function value rises, the long fine setting of small step is in the right direction. otherwise, change the long focusing of small step direction.
4) along the long focusing of small step direction finding drop point, return to the maximum of points recording in focusing process after finding focus function drop point, be correct focus point.
In experimentation after liquid lens driving voltage of every change, just utilize camera to catch present image, utilize autofocus evaluation function mentioned above to focus on evaluation simultaneously, climbing method as described above moves always, until the liquid lens driving voltage while finding figure image focu evaluation of estimate maximum, automatic focus completes.
Although illustrated and described example embodiment of the present invention, those skilled in the art are to be understood that, in the situation that the scope and spirit of the present invention that limit in not deviating from claim and equivalent thereof can be made the variation in various forms and details to these example embodiment.
Claims (3)
1. a Microscope Auto focus Method, the method device consists of optical microscope, liquid lens and driver module thereof, digital camera, computing machine and the software of automatically focusing, it is characterized in that: liquid lens is introduced in optical microscope light path, utilize liquid lens as zoom element, by controlling its two end electrodes voltage, change its diopter and cause whole optical system focal length variations.By video image is focused on, evaluate servocontrol liquid lens driving voltage to change, until video image focusing evaluation of estimate is the highest, image reaches the most clear state.
2. Microscope Auto focus Method according to claim 1, it is characterized in that: the focal length of whole microscope optical system is controlled by liquid lens driving voltage, when changing liquid lens driving voltage, its diopter changes and causes that whole micro optical system changes, fixed object is different, when continuously changing liquid lens driving voltage and making it when optimum focusing during liquid lens voltage, micro-image presents from fuzzy to clear again to fuzzy change procedure.
3. Microscope Auto focus Method according to claim 1, it is characterized in that: the software of automatically focusing is realized the integrated control functions to liquid lens focal length, camera image seizure, image quality evaluation, adopt auto focus evaluation function to carry out Real-Time Evaluation to image, and use focus search algorithm in real time liquid lens driving voltage to be carried out to servocontrol, make it converge to the liquid lens driving voltage in the time of can presenting optimum focusing image.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104834081A (en) * | 2015-04-10 | 2015-08-12 | 宁波大学 | Rapid automatic focusing method for stereoscopic microscope |
| CN105067619A (en) * | 2015-07-30 | 2015-11-18 | 苏州南光电子科技有限公司 | Digital microscope for food safety detection |
| CN105911557A (en) * | 2016-06-13 | 2016-08-31 | 天津大学 | Frequency modulated continuous wave laser ranging light beam automatic focusing device based on liquid lens |
| CN106094071A (en) * | 2016-06-13 | 2016-11-09 | 天津大学 | FM-CW laser ranging dual-beam quick focusing method based on liquid lens |
| CN106249325A (en) * | 2016-10-14 | 2016-12-21 | 北京信息科技大学 | A kind of bionical quick focus adjustment method of vision based on liquid lens |
| CN107077732A (en) * | 2014-08-27 | 2017-08-18 | 思迪赛特诊断有限公司 | System and method for calculating digital microscope focus variations |
| CN107272192A (en) * | 2016-04-01 | 2017-10-20 | 株式会社三丰 | Camera system and image capture method |
| CN110658570A (en) * | 2019-10-17 | 2020-01-07 | 天津大学 | Microscope system based on liquid zoom lens and microscopic imaging method thereof |
| CN111371995A (en) * | 2020-03-13 | 2020-07-03 | 北京理工大学 | Image acquisition method and device for observing animal neuron cell activity |
| CN111988590A (en) * | 2020-07-16 | 2020-11-24 | 歌尔光学科技有限公司 | Method and device for detecting definition of projection picture |
| CN112327479A (en) * | 2021-01-05 | 2021-02-05 | 北京卓立汉光仪器有限公司 | Optical imaging system and method for adjusting imaging parameters by programming |
| CN112532968A (en) * | 2019-09-18 | 2021-03-19 | 华为技术有限公司 | Surface shape calibration method for liquid lens in lens module |
| CN112965230A (en) * | 2021-02-26 | 2021-06-15 | 南京工程学院 | Microscope system and non-mechanical displacement automatic focusing method thereof |
| CN113109936A (en) * | 2021-04-08 | 2021-07-13 | 西南石油大学 | Microscope automatic focusing method and device based on image definition evaluation |
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Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107077732A (en) * | 2014-08-27 | 2017-08-18 | 思迪赛特诊断有限公司 | System and method for calculating digital microscope focus variations |
| CN107077732B (en) * | 2014-08-27 | 2020-11-24 | 思迪赛特诊断有限公司 | System and method for calculating focus variation for digital microscope |
| CN104834081A (en) * | 2015-04-10 | 2015-08-12 | 宁波大学 | Rapid automatic focusing method for stereoscopic microscope |
| CN105067619A (en) * | 2015-07-30 | 2015-11-18 | 苏州南光电子科技有限公司 | Digital microscope for food safety detection |
| CN107272192A (en) * | 2016-04-01 | 2017-10-20 | 株式会社三丰 | Camera system and image capture method |
| CN106094071A (en) * | 2016-06-13 | 2016-11-09 | 天津大学 | FM-CW laser ranging dual-beam quick focusing method based on liquid lens |
| CN106094071B (en) * | 2016-06-13 | 2017-12-12 | 天津大学 | FM-CW laser ranging dual-beam quick focusing method based on liquid lens |
| CN105911557A (en) * | 2016-06-13 | 2016-08-31 | 天津大学 | Frequency modulated continuous wave laser ranging light beam automatic focusing device based on liquid lens |
| CN106249325A (en) * | 2016-10-14 | 2016-12-21 | 北京信息科技大学 | A kind of bionical quick focus adjustment method of vision based on liquid lens |
| CN106249325B (en) * | 2016-10-14 | 2017-12-19 | 北京信息科技大学 | A kind of quick focus adjustment method of bionical vision based on liquid lens |
| CN112532968A (en) * | 2019-09-18 | 2021-03-19 | 华为技术有限公司 | Surface shape calibration method for liquid lens in lens module |
| CN112532968B (en) * | 2019-09-18 | 2021-09-14 | 华为技术有限公司 | Surface shape calibration method for liquid lens in lens module |
| CN110658570A (en) * | 2019-10-17 | 2020-01-07 | 天津大学 | Microscope system based on liquid zoom lens and microscopic imaging method thereof |
| CN111371995A (en) * | 2020-03-13 | 2020-07-03 | 北京理工大学 | Image acquisition method and device for observing animal neuron cell activity |
| CN111371995B (en) * | 2020-03-13 | 2021-06-25 | 北京理工大学 | Image acquisition method and device for observing animal neuron cell activity |
| CN111988590A (en) * | 2020-07-16 | 2020-11-24 | 歌尔光学科技有限公司 | Method and device for detecting definition of projection picture |
| CN111988590B (en) * | 2020-07-16 | 2022-06-21 | 歌尔光学科技有限公司 | Method and device for detecting definition of projection picture |
| CN112327479A (en) * | 2021-01-05 | 2021-02-05 | 北京卓立汉光仪器有限公司 | Optical imaging system and method for adjusting imaging parameters by programming |
| CN112965230A (en) * | 2021-02-26 | 2021-06-15 | 南京工程学院 | Microscope system and non-mechanical displacement automatic focusing method thereof |
| CN113109936A (en) * | 2021-04-08 | 2021-07-13 | 西南石油大学 | Microscope automatic focusing method and device based on image definition evaluation |
| CN113109936B (en) * | 2021-04-08 | 2022-03-11 | 西南石油大学 | Microscope automatic focusing method and device based on image definition evaluation |
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