CN103520777A - Highly-tough hole-adjustable gel artificial blood vessel and making method thereof - Google Patents
Highly-tough hole-adjustable gel artificial blood vessel and making method thereof Download PDFInfo
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Abstract
The invention relates to a highly-tough hole-adjustable gel artificial blood vessel and a making method thereof. The gel artificial blood vessel contains, by mass, 9-15% of an olefin monomer M1, 1-3% of a biological polymer M2, 0-0.02% of a chemical crosslinking agent M3 and 4-10% of an ionic crosslinking agent M4. The making method comprises the following steps: making a gel artificial blood vessel base by using the M1 and M2 as functional monomers and the M3 as a chemical crosslinking agent in an aqueous solution, and reinforcing by utilizing the M4 as an ionic crosslinking agent to make the highly-tough hole-adjustable gel artificial blood vessel. The gel artificial blood vessel made through the method has the advantages of high strength, toughness and resilience, difficult damage under the action of external forces comprising the pulling, bending, twisting and the like, rapid original shape recovery, multi-crosslinked network structure, and adjustment of the hole dimension through a crosslinking degree. The gel artificial blood vessel is suitable for the biological field, the medical field, the tissue engineering field, the adsorption field and the like.
Description
Technical field
The present invention relates to macromolecular material, biological medicine and field of tissue engineering technology, be specifically related to adjustable gel artificial blood vessel of a kind of high tenacity and hole and preparation method thereof.
Background technology
Blood vessel is the pipeline of pumping blood in organism, in human body except cornea, hair, refer to that (toe) first, dentine and epithelium etc. are located, blood vessel spreads all over whole body.Blood vessel can be divided into three kinds of tremulous pulse, vein and blood capillaries.Aorta and aortic tube wall are thicker, contain abundant elastic fiber, have expansivity and elasticity.The bore of blood capillary is minimum, and quantity is maximum, and total cross-sectional area is maximum, and blood flow rate is the slowest, and tube wall is the thinnest, only monolayer endothelial cell and basement membrane, consists of, and permeability is fine, is conducive to blood and tissue and carries out mass exchange.Along with the development of Medical Technology, needs are studied and grafting vessel, so people have invented artificial blood vessel, a kind of close with biological blood vessel in function and feature, can replace the artificial blood vessel of biological blood vessel.
Preparation artificial blood vessel need meet following requirement: 1. physics and chemistry stable performance; 2. mesh degree is suitable; 3. there is certain intensity and suppleness; 4. while making bypass surgery, easily seam property is good; 5. oozing of blood or oozing of blood is few and can at once stop not when blood vessel is connected blood-letting; 6. after moving into human body, tissue reaction is slight; 7. tissue can form rapidly newborn interior adventitia; 8. be difficult for forming thrombosis; 9. gratifying long-term patency rate.
The manufacture of artificial blood vessel's blood vessel, research both domestic and external, the overwhelming majority takes medical macromolecular materials to weave.Knitting, braiding and woven that woven method has.Be made into after tubing, through post processing, be processed into spiral helicine artificial blood vessel, can be arbitrarily crooked and unlikely suction is flat.China, at the end of the fifties, at the beginning of the sixties, just starts to study, and is to be originally made into nylon (Nylon), and rear Yin Nilong degraded is broken and is eliminated after raw body is implanted into.Adopt now terylene (Dacron) fibrage artificial blood vessels, oneself is widely used in clinical more, as treatment aortic aneurysm, and aortic stenosis, art etc., the longest 37cm that reaches are changed in superior and inferior vena cava excision.The artificial blood vessel who is woven by machine with macromolecular material at present, plain weave person internal diameter minimum is 8mm, knitting person's internal diameter is 3mm, but knitting artificial blood vessel not yet can formally produce at present at home.
Dacron-made artificial blood vessel is the vascular grafts using the earliest, and because patency rate is higher, is successfully used to for a long time trunk displacement, but cannot meet the manufacture requirement of small-caliber artificial blood vessel completely.After this, the raw material for the manufacture of artificial blood vessel develops into politef, polyurethane and natural mulberry silk.Occur take the straight type artificial blood vessel that high molecular weight ptfe is formed by injection molding as raw material the sixties, trade name is Cauer tank this (Core-Tex), has been widely used in clinical.Domestic and international application the most widely artificial blood vessel material is expanded polytetrafluoroethylsealing, it has good biocompatibility and anticoagulant, but compliance is poor, the patency rate of graft is only 30%, especially to be less than the above-mentioned shortcoming of politef (ePTFE) artificial blood vessel of 6mm more obvious for diameter, long-term patency rate extreme difference.
Now for the clinical artificial blood vessel who is mainly made by raw material synthetic high polymers such as polyethylene terephthalate (PET), expanded PTFE (ePTFE) and polyurethane (PU), polyethylene terephthalate is mainly that the form with polyster fibre is woven into tubulose, and endotheliocyte can be in the growth of interfibrous space; Blood vessel wall prepared by expanded PTFE has porous network structure, is conducive to endothelial cell growth.But terylene and expanded PTFE do not have biological activity and anticoagulating active, after transplanting, easily there is thrombosis.Polyurethane has good biocompatibility and blood compatibility, effectively antithrombotic.Therefore its synthetic high polymer can be met to a certain extent to preparation artificial blood vessel's condition, be applied to clinical.
By upper analysis, portion's circulation is bad within it for above-mentioned artificial blood vessel's ubiquity blood, the shortcoming such as biocompatibility and poor mechanical property, is necessary to find a kind of artificial blood vessel with good biological vascular performance, will fully meet the requirement of transplanting in combination property.
Chinese patent CN101284148A discloses a kind of artificial blood vessel of preparation method, one or more in Kynoar (PVDF), polyether sulfone (PES) and polysulfones (PS) of formula form, be prepared into high polymer, passed through swelling and dissolved configuration film forming liquid, then filter deaeration and send out and prepare hollow-fibre membrane through dry-jet wet-spinning, then make blood vessel through post-processed.Yet this preparation method relates to toxic solvent, organism is had to certain toxicity, and making step is loaded down with trivial details, need a series of operation, be difficult for preparation.
Chinese patent CN1313741A discloses a kind of artificial blood vessel who utilizes heterologous organism blood vessel and form with the cell component of removing this blood vessel after surfactant washing blood vessel completely.This method raw material is difficult to obtain, and deals with inconvenience, can not produce in enormous quantities.
Macromolecule hydrogel is the high polymer that a class has three-dimensional net structure.Because having unique water conservation, mechanical property and good biocompatibility, it is widely used in the fields such as biological engineering, medicines and health protection.
Gel, as a kind of novel artificial blood vessel material, itself is the soft wet stock similar to human body soft tissue, suitablely in human body environment, applies.Gel has that chemical constitution is clear and definite, stable performance and be easy to regulation and control, foreign infect, cheap and be easy to the advantages such as sterilizing; By changing the regulation and control of mechanism of crosslinking and proportioning, can realize the mechanical property of high-intensity high-tenacity and good visible light permeability energy, for gel provides mechanics guarantee and optics support in the application of vascular grafts, in addition, the degradation resistant of gel macromolecular material and weatherability make material in being applied to the process of human body, be difficult for degraded and rejection occur, and the materials'use cycle is longer.
The present invention has considered that the preparation of artificial blood vessel need to meet avirulence, biocompatibility, select permeability and good mechanical properties, and manufacture the features such as simple and cost is low, use the feature of macromolecule composite aquogel to be prepared gel artificial blood vessel, alternative PET, ePTFE and the PU artificial blood vessel who uses clinically, gathers around and has wide practical use at medical domain.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of biocompatibility having had is provided, have the artificial blood vessel material of high-strength and high ductility and optical transmission simultaneously concurrently.The intensity of the vascular grafts that the present invention obtains is high, good toughness; at biological existence temperature, can meet the required actions such as various tension and compression bendings of biological daily routines; be beneficial to the propagation of vascular endothelial cell; and visible ray optical transmittance and recoverability are good, can meet the daily medical requirements such as transfusion, blood drawing.This material is to organism nonhazardous effect, good biocompatibility, and there is the advantages such as degradation resistant, be beneficial in human body and use for a long time.
The present invention is achieved by the following technical solutions:
The gel artificial blood vessel that a kind of high tenacity and hole are adjustable be by olefin monomer M1, biopolymer M2, chemical cross-linking agent M3, ion crosslinking agent M4 is water-soluble is polymerized.
Described olefin monomer M1 is acrylamide, Methacrylamide, NIPA, N hydroxymethyl acrylamide, 2-(dimethylamino) ethyl-Methacrylamide, 2-hydroxyethyl methacrylate, acrylic acid, methacrylic acid, tert-butyl acrylate, sodium acrylate, 2-acrylamide-2-methyl isophthalic acid-propane sulfonic acid, Polyethyleneglycol methacrylate, its inorganic salt, with and composition thereof.
Described biopolymer M2 is the mixture of a kind of or any two kinds of compositions in sodium alginate, chitosan, xanthan gum, humic acid, cellulose, polylactic acid, polylysine, polyglutamic acid, polyvinyl alcohol, polyhydroxyalkanoate, collagen protein, sericin shape globulin.
Described chemical cross-linking agent M3 is N, any one in N '-methylene-bisacrylamide, two (methyl) acrylic acid glycol ester, diglycidyl ether.
Described ion crosslinking agent M4 is any one in calcium chloride, copper chloride, zinc chloride, calcium bisulfate, copper sulfate, zinc sulfate.
The preparation method of the gel artificial blood vessel that a kind of high tenacity of the present invention and hole are adjustable, comprises the steps:
1) preparation of A liquid: at 25 ℃, olefin monomer M1, biopolymer M2 are joined in deionized water successively, be mixed with the solution that mass concentration is 10-18%, stir 5-30min, discontinuous degassing 15-30min;
2) preparation of B liquid: at 25 ℃, chemical cross-linking agent M3 and initiator ammonium persulfate or potassium peroxydisulfate are joined in deionized water successively, magnetic agitation 5-10min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 10-20min, when presenting homogeneous thick, adds catalyst tetramethylethylenediamine or sodium sulfite immediately, magnetic agitation 5-10min, makes pre-polymerization liquid;
4) the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects hollow tubular mould, by being to carry out polyreaction 3-8h at 40-70 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the gel artificial blood pipe intensive treatment of gel artificial blood vessel base: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of ion crosslinking agent M4 of 4-10%, response time is 15-60min, after taking out, with deionized water soaking flushing 1-5min, make the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Step 1 of the present invention) in, each constituent mass percentage ratio of A liquid is: olefin monomer M1:9-15%, biopolymer M2:1-3%, deionized water: 82-90%.
Step 2 of the present invention) in, each constituent mass ratio of B liquid is: chemical cross-linking agent M3:0-0.2 ‰, initiator are 1% of olefin monomer M1, deionized water: 98-99%.
Step 3 of the present invention) catalyst in is 3 ‰ of olefin monomer M1.
Step 5 of the present invention) in, each constituent mass ratio is: ion crosslinking agent M4:4-10%, deionized water: 90-96%.
The internal diameter of described hollow tubular mould is that 0.1-15mm, external diameter are 0.1-17mm.
Described step 5) not the essential condition of preparing gel artificial blood vessel, but can increase fracture strength and the hot strength of gel artificial blood vessel.
The hole size of described gel artificial blood vessel is 10nm-500 μ m.
The toughness of described gel artificial blood vessel, bore hole size, the endotheliocyte compatibility and patency rate are by regulating monomer concentration, rate of charge, the degree of cross linking, reaction temperature, mould size and charging sequence to regulate.
Compare with preparation method with existing artificial blood vessel material, the present invention has following advantage:
1, there is higher intensity, toughness and recovery, cracky not under the External Force Actings such as tension and compression bending, and can resile fast.
2, have good biocompatibility, resulting materials is nontoxic, is applicable to the replacement of inside of human body organization material.
3, have multiple cross-linked network structure, bore hole size can be regulated by the degree of cross linking, and has high life cycle and life-span.
4, preparation method of the present invention is simple, raw material is easy to get, easy and simple to handle, cost is low, reaction time is short, reaction condition is gentle, is easy to industrialization and mass production applications.
5, the internal diameter size of prepared gel blood vessel and wall thickness can regulate according to die size, to meet the performance requirement of the different classes of blood vessels such as elastic artery, muscle tremulous pulse, small artery.
Specific embodiments
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention and principal character.Therefore; following specific embodiments is only not used in and limits the scope of the invention for the present invention is described; other any do not run counter to change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
1) preparation of A liquid: by 1g acrylamide, 0.1g sodium alginate, join successively in deionized water at 25 ℃, be mixed with mass concentration and be 10% solution, stir 15min, discontinuous degassing 15min;
2) preparation of B liquid: at 25 ℃,, by 0.001gN, N '-methylene-bisacrylamide and 0.1g Ammonium persulfate. join in deionized water successively, magnetic agitation 5min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 10min, when presenting homogeneous thick, adds 0.002g tetramethylethylenediamine immediately, and magnetic agitation 7min, makes pre-polymerization liquid;
4) it is that 0.2mm external diameter is the hollow tubular mould of 0.3mm that the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects internal diameter, by being to carry out polyreaction 6h at 40 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the hollow blood vessel base intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of 4% zinc chloride, response time is 30min, after taking out, with deionized water soaking flushing 3min, make the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Embodiment 2
1) preparation of A liquid: at 25 ℃, 1.2g N hydroxymethyl acrylamide, 0.15g sodium alginate are joined in deionized water successively, be mixed with mass concentration and be 12% solution, stir 20min, discontinuous degassing 30min;
2) preparation of B liquid: at 25 ℃,, by 0.0015gN, N '-methylene-bisacrylamide and 0.012g potassium peroxydisulfate join in deionized water successively, magnetic agitation 10min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 15min, when presenting homogeneous thick, adds 0.0036g tetramethylethylenediamine immediately, and magnetic agitation 8min, makes pre-polymerization liquid;
4) it is that 0.5mm external diameter is the hollow tubular mould of 0.65mm that the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects internal diameter, by being to carry out polyreaction 5h at 50 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the hollow blood vessel base intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of 7% copper sulfate, response time is 20min, after taking out, with deionized water soaking flushing 5min, make the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Embodiment 3
1) preparation of A liquid: at 25 ℃, 1.5g Methacrylamide, 0.1g sodium alginate are joined in deionized water successively, be mixed with mass concentration and be 14% solution, stir 30min, discontinuous degassing 30min;
2) preparation of B liquid: at 25 ℃, 0.0015g bis-(methyl) acrylic acid glycol ester and 0.015g potassium peroxydisulfate are joined in deionized water successively, magnetic agitation 5min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 10min, when presenting homogeneous thick, adds 0.0045g methyl ethylenediamine immediately, and magnetic agitation 5min, makes pre-polymerization liquid;
4) it is that 1mm external diameter is the hollow tubular mould of 1.2mm that the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects internal diameter, by being to carry out polyreaction 5h at 50 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the hollow blood vessel base intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of 7% calcium bisulfate, response time is 30min, after taking out, with deionized water soaking flushing 4min, make the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Embodiment 4
1) preparation of A liquid: at 25 ℃, 1.4g acrylamide, 0.2g sericin shape globulin are joined in deionized water successively, be mixed with mass concentration and be 14% solution, stir 25min, discontinuous degassing 15min;
2) preparation of B liquid: at 25 ℃,, by 0.0020gN, N '-methylene-bisacrylamide and 0.014g Ammonium persulfate. join in deionized water successively, magnetic agitation 10min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 20min, when presenting homogeneous thick, adds catalyst 0.0042g tetramethylethylenediamine immediately, and magnetic agitation 7min, makes pre-polymerization liquid;
4) it is that 6mm external diameter is the hollow tubular mould of 7mm that the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects internal diameter, by being to carry out polyreaction 4h at 60 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the hollow blood vessel base intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in 5% calcium chloride water, response time is 20min, after taking out, with deionized water soaking flushing 3min, makes the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Embodiment 5
1) preparation of A liquid: at 25 ℃, 1.6g NIPA, 0.2g cellulose are joined in deionized water successively, be mixed with mass concentration and be 16% solution, stir 25min, discontinuous degassing 30min;
2) preparation of B liquid: at 25 ℃,, by 0.0015gN, N '-methylene-bisacrylamide and 0.016g potassium peroxydisulfate join in deionized water successively, magnetic agitation 8min, until form the dispersion of transparent and homogeneous;
3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 20min, when presenting homogeneous thick, adds 0.0048g sodium sulfite immediately, and magnetic agitation 10min, makes pre-polymerization liquid;
4) it is that 3mm external diameter is the hollow tubular mould of 3.5mm that the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects internal diameter, by being to carry out polyreaction 6h at 55 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;
5) the hollow blood vessel base intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of 9% calcium chloride, response time is 40min, after taking out, with deionized water soaking flushing 2min, make the adjustable gel artificial blood vessel of high tenacity and hole;
6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
Claims (8)
- A high tenacity and the adjustable gel artificial blood vessel of hole be by olefin monomer M1, biopolymer M2, chemical cross-linking agent M3, ion crosslinking agent M4 is water-soluble is polymerized; Described olefin monomer M1 is acrylamide, Methacrylamide, NIPA, N hydroxymethyl acrylamide, 2-(dimethylamino) ethyl-Methacrylamide, 2-hydroxyethyl methacrylate, acrylic acid, methacrylic acid, tert-butyl acrylate, sodium acrylate, 2-acrylamide-2-methyl isophthalic acid-propane sulfonic acid, Polyethyleneglycol methacrylate, its inorganic salt, with and composition thereof; Described biopolymer M2 is the mixture of a kind of or any two kinds of compositions in sodium alginate, chitosan, xanthan gum, humic acid, cellulose, polylactic acid, polylysine, polyglutamic acid, polyvinyl alcohol, polyhydroxyalkanoate, collagen protein, sericin shape globulin; Described chemical cross-linking agent M3 is N, any one in N'-methylene-bisacrylamide, two (methyl) acrylic acid glycol ester, diglycidyl ether; Described ion crosslinking agent M4 is any one in calcium chloride, copper chloride, zinc chloride, calcium bisulfate, copper sulfate, zinc sulfate.
- 2. a preparation method for the adjustable gel artificial blood vessel of high tenacity and hole, comprises the steps:1) preparation of A liquid: at 25 ℃, olefin monomer M1, biopolymer M2 are joined in deionized water successively, be mixed with the solution that mass concentration is 10-18%, stir 5-30min, discontinuous degassing 15-30min.2) preparation of B liquid: at 25 ℃, chemical cross-linking agent M3 and initiator ammonium persulfate or potassium peroxydisulfate are joined in deionized water successively, magnetic agitation 5-10min, until form the dispersion of transparent and homogeneous;3) being mixed of pre-polymerization liquid: by step 2) the B liquid of preparation is poured step 1 into) in the A liquid that makes, magnetic agitation 10-20min, when presenting homogeneous thick, adds catalyst tetramethylethylenediamine or sodium sulfite immediately, magnetic agitation 5-10min, makes pre-polymerization liquid;4) the pre-polymerization liquid preparation of gel artificial blood pipe: by step 3) making injects hollow tubular mould, by being to carry out polyreaction 3-8h at 40-70 ℃ in temperature after the sealing of port, obtains gel artificial blood pipe;5) the gel artificial blood pipe intensive treatment of gel artificial blood pipe: by step 4) making takes out, be immersed in mass concentration and be in the aqueous solution of ion crosslinking agent M4 of 4-10%, response time is 15-60min, after taking out, with deionized water soaking flushing 1-5min, make the adjustable gel artificial blood vessel of high tenacity and hole;6) the gel artificial blood vessel packing of gel artificial blood vessel: by step 5) making carries out aseptic vacuum heat-seal packing.
- 3. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 2 and hole, is characterized in that: step 1) in each constituent mass percentage ratio of A liquid be: olefin monomer M1:9-15%, biopolymer M2:1-3%, ion crosslinking agent M4:0-10 ‰, deionized water: 82-90%; Step 2) in, each constituent mass ratio of B liquid is: chemical cross-linking agent M3:0-0.2 ‰, initiator are 1% of olefin monomer M1, deionized water: 98-99%; Step 3) catalyst in is 3 ‰ of olefin monomer M1; Step 5) in, each constituent mass matches well than being: ion crosslinking agent M4:4-10%, deionized water: 90-96%.
- 4. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 2 and hole, is characterized in that: the internal diameter of described hollow tubular mould is that 0.1-15mm, external diameter are 0.1-17mm.
- 5. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 2 and hole, it is characterized in that: be not described step 5) essential condition of preparing gel artificial blood vessel, but can increase fracture strength and the hot strength of gel artificial blood vessel.
- 6. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 2 and hole, is characterized in that: the toughness of described gel artificial blood vessel, bore hole size, the endotheliocyte compatibility and patency rate are by regulating monomer concentration, rate of charge, the degree of cross linking, reaction temperature, mould size and charging sequence to regulate.
- 7. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 2 and hole, is characterized in that: the hole size of described gel artificial blood vessel is 10nm-500 μ m.
- 8. the preparation method of the adjustable gel artificial blood vessel of a kind of high tenacity according to claim 1 and hole, it is characterized in that: the internal diameter size of prepared gel blood vessel and wall thickness can change die size and regulate, to meet the performance requirement of the different classes of blood vessels such as elastic artery, muscle tremulous pulse, small artery.
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